Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,405,946 T→G 100% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,405,9460TG99.6% 977.2 / ‑5.2 241Q43P (CAG→CCG) yfbUUPF0304 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (124/114);  total (124/117)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

GTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCA  >  CP009273/2405802‑2406090
                                                                                                                                                |                                                                                                                                                
   acacGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATc                                                                                                                                            <  2:2993843/148‑1 (MQ=255)
      cgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGt                                                                                                                                         <  1:362540/148‑1 (MQ=255)
      cgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGt                                                                                                                                         >  2:2430073/1‑148 (MQ=255)
       gacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTa                                                                                                                                        <  1:1694516/148‑1 (MQ=255)
        acgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTaa                                                                                                                                       <  1:1562722/148‑1 (MQ=255)
          gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAcc                                                                                                                                     <  1:2559134/148‑1 (MQ=255)
           ttCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCa                                                                                                                                    >  2:161228/1‑148 (MQ=255)
             cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACg                                                                                                                                  <  1:2162965/148‑1 (MQ=255)
              aTCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGc                                                                                                                                 <  1:2978278/148‑1 (MQ=255)
                  aTGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                             >  2:909365/1‑148 (MQ=255)
                  aTGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                             >  1:2296192/1‑148 (MQ=255)
                  aTGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                             >  2:1726534/1‑148 (MQ=255)
                    ggATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaata                                                                                                                           <  2:2123714/148‑1 (MQ=255)
                     gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaataa                                                                                                                          >  1:2023056/1‑148 (MQ=255)
                     gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaataa                                                                                                                          <  1:1389610/148‑1 (MQ=255)
                       ttGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATATACATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCACTTCACGCATCGGTAATCCGTAACCACGCTCAATAAtt                                                                                                                        <  1:3416916/148‑1 (MQ=255)
                            ggTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTg                                                                                                                   >  1:3327186/1‑148 (MQ=255)
                            ggTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTg                                                                                                                   <  2:2998189/148‑1 (MQ=255)
                               ccTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAg                                                                                                                >  2:360868/1‑148 (MQ=255)
                                 tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGc                                                                                                              <  1:219330/148‑1 (MQ=255)
                                  gCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCg                                                                                                             >  2:209425/1‑148 (MQ=255)
                                     aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                          >  2:425103/1‑148 (MQ=255)
                                      aTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACgg                                                                                                         >  1:2147347/1‑148 (MQ=255)
                                       ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                        <  1:914422/148‑1 (MQ=255)
                                       ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                        <  2:1140092/148‑1 (MQ=255)
                                       ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                        <  2:22813/148‑1 (MQ=255)
                                        tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTa                                                                                                       >  2:2879425/1‑148 (MQ=255)
                                        tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTa                                                                                                       >  2:2684046/1‑148 (MQ=255)
                                          gACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                     >  2:1848058/1‑148 (MQ=255)
                                             cAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGtt                                                                                                  <  2:3064397/148‑1 (MQ=255)
                                              aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTc                                                                                                 >  1:2356906/1‑148 (MQ=255)
                                                gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                               >  2:3319476/1‑148 (MQ=255)
                                                gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                               >  2:3218566/1‑148 (MQ=255)
                                                gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                               >  1:1763685/1‑148 (MQ=255)
                                                  aaCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCa                                                                                             >  2:708358/1‑148 (MQ=255)
                                                   aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAt                                                                                            >  1:2244812/1‑148 (MQ=255)
                                                    cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAtt                                                                                           <  2:1296938/148‑1 (MQ=255)
                                                    cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAtt                                                                                           >  2:1808061/1‑148 (MQ=255)
                                                    cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAtt                                                                                           <  2:866371/148‑1 (MQ=255)
                                                      tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                         >  1:1640013/1‑148 (MQ=255)
                                                      tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                         <  2:2291883/148‑1 (MQ=255)
                                                       gCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGc                                                                                        >  1:2107448/1‑148 (MQ=255)
                                                        cAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGcc                                                                                       >  2:2800465/1‑148 (MQ=255)
                                                         aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCg                                                                                      <  1:3193063/148‑1 (MQ=255)
                                                          aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCgg                                                                                     >  2:2155432/1‑148 (MQ=255)
                                                          aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCgg                                                                                     <  2:2248258/148‑1 (MQ=255)
                                                           cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                    <  1:3530294/148‑1 (MQ=255)
                                                           cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                    >  1:629094/1‑148 (MQ=255)
                                                            gCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGAt                                                                                   <  2:173902/148‑1 (MQ=255)
                                                              aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCg                                                                                 <  2:1640013/148‑1 (MQ=255)
                                                                  tACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCa                                                                             >  2:744354/1‑148 (MQ=255)
                                                                     aTCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAg                                                                          >  1:1128699/1‑148 (MQ=255)
                                                                     aTCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAg                                                                          >  1:2903659/1‑148 (MQ=255)
                                                                        tcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtca                                                                       >  1:2036982/1‑148 (MQ=255)
                                                                          cATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatc                                                                     <  2:1111685/148‑1 (MQ=255)
                                                                            tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTcatcat                                                                   >  1:563878/1‑148 (MQ=255)
                                                                               tGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                >  1:701108/1‑148 (MQ=255)
                                                                                gTCGATGATAGTGCTGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTg                                                               >  1:1012485/1‑148 (MQ=255)
                                                                                 tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGt                                                              <  1:708172/148‑1 (MQ=255)
                                                                                 tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGt                                                              <  1:3258312/148‑1 (MQ=255)
                                                                                  cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTa                                                             <  2:2023056/148‑1 (MQ=255)
                                                                                    atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACt                                                           <  1:314754/148‑1 (MQ=255)
                                                                                      gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGa                                                         <  2:3358374/148‑1 (MQ=255)
                                                                                       atAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                        <  1:1166678/148‑1 (MQ=255)
                                                                                       atAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                        <  1:3174906/148‑1 (MQ=255)
                                                                                        tAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                       >  2:2177920/1‑148 (MQ=255)
                                                                                        tAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                       <  1:161228/148‑1 (MQ=255)
                                                                                        tAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                       >  2:2396287/1‑148 (MQ=255)
                                                                                         aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTa                                                      >  2:1028483/1‑148 (MQ=255)
                                                                                         aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTa                                                      >  2:387268/1‑148 (MQ=255)
                                                                                         aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTa                                                      >  2:388958/1‑148 (MQ=255)
                                                                                           tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                    >  1:2694152/1‑148 (MQ=255)
                                                                                           tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                    <  2:3127896/148‑1 (MQ=255)
                                                                                             cGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACa                                                  <  1:460257/148‑1 (MQ=255)
                                                                                               gCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaa                                                <  2:2282491/148‑1 (MQ=255)
                                                                                                cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaaa                                               <  1:2632748/148‑1 (MQ=255)
                                                                                                cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaaa                                               >  1:2488154/1‑148 (MQ=255)
                                                                                                 aGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAt                                              <  2:798215/148‑1 (MQ=255)
                                                                                                    tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAg                                           >  1:2751609/1‑148 (MQ=255)
                                                                                                       cttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACg                                        >  2:2330007/1‑148 (MQ=255)
                                                                                                        ttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGt                                       >  2:946/1‑148 (MQ=255)
                                                                                                        ttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGt                                       >  2:1531365/1‑148 (MQ=255)
                                                                                                          cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTg                                     <  1:1726877/148‑1 (MQ=255)
                                                                                                             tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcgc                                  >  2:70318/1‑148 (MQ=255)
                                                                                                               aGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                >  2:289225/1‑148 (MQ=255)
                                                                                                               aGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                >  2:289359/1‑148 (MQ=255)
                                                                                                                gCTCGCCAAACTCGCGATCCAATTCACGCCTCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGtt                               >  2:2562149/1‑148 (MQ=255)
                                                                                                                 cTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                              >  2:1178817/1‑148 (MQ=255)
                                                                                                                   cGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGt                            <  1:1848058/148‑1 (MQ=255)
                                                                                                                      cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAt                         >  2:3412685/1‑148 (MQ=255)
                                                                                                                      cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAt                         <  1:2879425/148‑1 (MQ=255)
                                                                                                                      cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAt                         <  2:446361/148‑1 (MQ=255)
                                                                                                                        aaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAttt                       >  1:1715858/1‑148 (MQ=255)
                                                                                                                           tCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCa                    <  2:1124280/148‑1 (MQ=255)
                                                                                                                              cgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttg                 >  1:2179447/1‑148 (MQ=255)
                                                                                                                                 tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg              <  2:1203852/148‑1 (MQ=255)
                                                                                                                                 tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg              >  2:1383710/1‑148 (MQ=255)
                                                                                                                                      ttCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTcc         <  1:97226/148‑1 (MQ=255)
                                                                                                                                          cGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTa     <  1:2804131/148‑1 (MQ=255)
                                                                                                                                             aTCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa  <  2:526400/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCA  >  CP009273/2405802‑2406090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCT  >  CP009273/2405801‑2406094
                                                                                                                                                 |                                                                                                                                                    
GGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAA                                                                                                                                                  >  SRR19973067.2856405/1‑150 (MQ=60)
       CGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAA                                                                                                                                           <  SRR19973067.365057/150‑1 (MQ=60)
        GACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAC                                                                                                                                          <  SRR19973067.1706237/150‑1 (MQ=60)
        GACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAC                                                                                                                                          <  SRR19973067.1706179/150‑1 (MQ=60)
         ACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACC                                                                                                                                         <  SRR19973067.1573408/150‑1 (MQ=60)
          CGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCA                                                                                                                                        <  SRR19973067.1627519/150‑1 (MQ=60)
           GTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAC                                                                                                                                       <  SRR19973067.2575861/150‑1 (MQ=60)
           GTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAC                                                                                                                                       >  SRR19973067.2297584/1‑150 (MQ=60)
              CATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCT                                                                                                                                    <  SRR19973067.2177581/150‑1 (MQ=60)
              CATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCT                                                                                                                                    <  SRR19973067.2177423/150‑1 (MQ=60)
               ATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTC                                                                                                                                   <  SRR19973067.2997080/150‑1 (MQ=60)
                 CGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAA                                                                                                                                 >  SRR19973067.2311342/1‑150 (MQ=60)
                    TGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAA                                                                                                                              >  SRR19973067.2036637/1‑150 (MQ=60)
                      GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATT                                                                                                                            <  SRR19973067.1399056/150‑1 (MQ=60)
                        TTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGT                                                                                                                          <  SRR19973067.3438637/150‑1 (MQ=60)
                        TTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATATACATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCACTTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGT                                                                                                                          <  SRR19973067.3438665/150‑1 (MQ=60)
                           CTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTG                                                                                                                       <  SRR19973067.992491/150‑1 (MQ=60)
                           CTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTG                                                                                                                       >  SRR19973067.3348233/1‑150 (MQ=60)
                             GGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCA                                                                                                                     >  SRR19973067.326098/1‑150 (MQ=60)
                              GTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAG                                                                                                                    <  SRR19973067.296335/150‑1 (MQ=60)
                              GTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAG                                                                                                                    >  SRR19973067.3175253/1‑150 (MQ=60)
                               TCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGG                                                                                                                   <  SRR19973067.3073990/150‑1 (MQ=60)
                                  TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGA                                                                                                                <  SRR19973067.220939/150‑1 (MQ=60)
                                  TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGA                                                                                                                <  SRR19973067.221104/150‑1 (MQ=60)
                                   GCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAC                                                                                                               <  SRR19973067.2126357/150‑1 (MQ=60)
                                     AAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGG                                                                                                             >  SRR19973067.2161703/1‑150 (MQ=60)
                                      AATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGT                                                                                                            <  SRR19973067.707295/150‑1 (MQ=60)
                                      AATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGT                                                                                                            >  SRR19973067.1617884/1‑150 (MQ=60)
                                      AATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGT                                                                                                            >  SRR19973067.1617872/1‑150 (MQ=60)
                                        TTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAA                                                                                                          <  SRR19973067.920512/150‑1 (MQ=60)
                                         TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                         >  SRR19973067.529943/1‑150 (MQ=60)
                                            ACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTT                                                                                                      >  SRR19973067.1759406/1‑150 (MQ=60)
                                             CCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTC                                                                                                     >  SRR19973067.2372278/1‑150 (MQ=60)
                                              CAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCA                                                                                                    >  SRR19973067.1327744/1‑150 (MQ=60)
                                               AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                   >  SRR19973067.1948508/1‑150 (MQ=60)
                                               AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                   >  SRR19973067.1776755/1‑150 (MQ=60)
                                               AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                   >  SRR19973067.1775683/1‑150 (MQ=60)
                                                  AAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAT                                                                                                >  SRR19973067.2259727/1‑150 (MQ=60)
                                                  AAACATGCAACGCATGATACATCTCCATAATGTCGAGGATAGTGCGGCAGGTTTGTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAT                                                                                                >  SRR19973067.2259731/1‑150 (MQ=60)
                                                    ACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTT                                                                                              <  SRR19973067.3577538/150‑1 (MQ=60)
                                                     CATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTG                                                                                             >  SRR19973067.45658/1‑150 (MQ=60)
                                                     CATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTG                                                                                             >  SRR19973067.1651308/1‑150 (MQ=60)
                                                      ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                            >  SRR19973067.2121445/1‑150 (MQ=60)
                                                      ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                            >  SRR19973067.2960071/1‑150 (MQ=60)
                                                      ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                            >  SRR19973067.2078446/1‑150 (MQ=60)
                                                          AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                        >  SRR19973067.634059/1‑150 (MQ=60)
                                                          AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                        >  SRR19973067.633289/1‑150 (MQ=60)
                                                          AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                        <  SRR19973067.3213225/150‑1 (MQ=60)
                                                          AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                        <  SRR19973067.110260/150‑1 (MQ=60)
                                                            CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                      <  SRR19973067.3552601/150‑1 (MQ=60)
                                                            CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                      <  SRR19973067.978251/150‑1 (MQ=60)
                                                            CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                      <  SRR19973067.3552614/150‑1 (MQ=60)
                                                            CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                      <  SRR19973067.2041806/150‑1 (MQ=60)
                                                            CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                      <  SRR19973067.2041935/150‑1 (MQ=60)
                                                               ATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAG                                                                                   >  SRR19973067.2554029/1‑150 (MQ=60)
                                                               ATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAG                                                                                   >  SRR19973067.1234272/1‑150 (MQ=60)
                                                                   TACATCTCCATACTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATA                                                                               <  SRR19973067.2792998/150‑1 (MQ=60)
                                                                    ACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAG                                                                              >  SRR19973067.1136316/1‑150 (MQ=60)
                                                                    ACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAG                                                                              >  SRR19973067.2922172/1‑150 (MQ=60)
                                                                       TCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCA                                                                           >  SRR19973067.2050618/1‑150 (MQ=60)
                                                                           CATAATGTCGATGATAGTGCGGCAGTTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCTATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCAT                                                                       <  SRR19973067.2651440/150‑1 (MQ=60)
                                                                           CATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCAT                                                                       >  SRR19973067.1683717/1‑150 (MQ=60)
                                                                           CATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCAT                                                                       >  SRR19973067.567552/1‑150 (MQ=60)
                                                                              AATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTT                                                                    >  SRR19973067.705956/1‑150 (MQ=60)
                                                                               ATGTCGATGATAGTGCTGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTG                                                                   >  SRR19973067.1019439/1‑150 (MQ=60)
                                                                               ATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGATCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTG                                                                   <  SRR19973067.3522210/150‑1 (MQ=60)
                                                                                  TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                <  SRR19973067.713258/150‑1 (MQ=60)
                                                                                  TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                <  SRR19973067.3278672/150‑1 (MQ=60)
                                                                                  TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                <  SRR19973067.713071/150‑1 (MQ=60)
                                                                                  TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                <  SRR19973067.2148902/150‑1 (MQ=60)
                                                                                     ATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGA                                                             <  SRR19973067.316861/150‑1 (MQ=60)
                                                                                     ATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGA                                                             >  SRR19973067.3285805/1‑150 (MQ=60)
                                                                                        ATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTA                                                          <  SRR19973067.2916560/150‑1 (MQ=60)
                                                                                        ATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTA                                                          <  SRR19973067.3194676/150‑1 (MQ=60)
                                                                                        ATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTA                                                          <  SRR19973067.3194997/150‑1 (MQ=60)
                                                                                        ATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTA                                                          <  SRR19973067.1174428/150‑1 (MQ=60)
                                                                                         TAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAG                                                         <  SRR19973067.162562/150‑1 (MQ=60)
                                                                                          AGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGA                                                        >  SRR19973067.2711478/1‑150 (MQ=60)
                                                                                              CGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAA                                                    <  SRR19973067.463443/150‑1 (MQ=60)
                                                                                               GGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAA                                                   >  SRR19973067.2503786/1‑150 (MQ=60)
                                                                                               GGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAA                                                   >  SRR19973067.2504508/1‑150 (MQ=60)
                                                                                                 CAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATA                                                 <  SRR19973067.2649786/150‑1 (MQ=60)
                                                                                                  AGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAA                                                >  SRR19973067.2715774/1‑150 (MQ=60)
                                                                                                   GGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAG                                               >  SRR19973067.692942/1‑150 (MQ=60)
                                                                                                   GGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAG                                               >  SRR19973067.3207089/1‑150 (MQ=60)
                                                                                                   GGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAG                                               >  SRR19973067.2769618/1‑150 (MQ=60)
                                                                                                   GGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAG                                               >  SRR19973067.2769391/1‑150 (MQ=60)
                                                                                                         TTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTG                                         >  SRR19973067.2195107/1‑150 (MQ=60)
                                                                                                           CTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCG                                       <  SRR19973067.1738621/150‑1 (MQ=60)
                                                                                                           CTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCG                                       <  SRR19973067.1738707/150‑1 (MQ=60)
                                                                                                                    CGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                              <  SRR19973067.3340469/150‑1 (MQ=60)
                                                                                                                    CGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                              <  SRR19973067.1860654/150‑1 (MQ=60)
                                                                                                                    CGCCAAACTCGCGATCCAAGTCACTCATCGGTAATCCGTAACCACTCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                              <  SRR19973067.3340553/150‑1 (MQ=60)
                                                                                                                       CAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTT                           <  SRR19973067.2897847/150‑1 (MQ=60)
                                                                                                                       CAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTT                           >  SRR19973067.1727619/1‑150 (MQ=60)
                                                                                                                        AAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTC                          <  SRR19973067.217856/150‑1 (MQ=60)
                                                                                                                        AAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTC                          >  SRR19973067.631859/1‑150 (MQ=60)
                                                                                                                        AAACTCGCGATCCAATTCACGCATCAGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTC                          <  SRR19973067.532359/150‑1 (MQ=60)
                                                                                                                         AACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCC                         <  SRR19973067.1169798/150‑1 (MQ=60)
                                                                                                                           CTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAT                       >  SRR19973067.1527710/1‑150 (MQ=60)
                                                                                                                            TCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATT                      <  SRR19973067.2631539/150‑1 (MQ=60)
                                                                                                                             CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                     >  SRR19973067.2194022/1‑150 (MQ=60)
                                                                                                                             CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                     >  SRR19973067.2194058/1‑150 (MQ=60)
                                                                                                                             CGCGATCCAAGTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                     <  SRR19973067.2428739/150‑1 (MQ=60)
                                                                                                                               CGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                   >  SRR19973067.2676603/1‑150 (MQ=60)
                                                                                                                               CGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                   >  SRR19973067.889281/1‑150 (MQ=60)
                                                                                                                                  TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                <  SRR19973067.1707473/150‑1 (MQ=60)
                                                                                                                                      ATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCT            <  SRR19973067.1739236/150‑1 (MQ=60)
                                                                                                                                       TTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTG           <  SRR19973067.2322209/150‑1 (MQ=60)
                                                                                                                                       TTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTG           <  SRR19973067.98230/150‑1 (MQ=60)
                                                                                                                                           CGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATC       <  SRR19973067.2822215/150‑1 (MQ=60)
                                                                                                                                                CGGTAATCCGTAACCACGCACAATAATTTTTGGCAGTAGTCGGTAACGTTCAGCATTTGCCTGATCGAGCATAGTCAACATCTTGTACTGATTAGACATTTTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCT  <  SRR19973067.2694352/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
GGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCT  >  CP009273/2405801‑2406094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑