Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,715,643 G→T 92.4% E778* (GAA→TAA)  pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,715,6430GT92.4% 873.8 / 52.9 279E778* (GAA→TAA) pkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (19/3);  new base T (128/129);  total (147/132)
Fisher's exact test for biased strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 280 total aligned reads displayed.

CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTT  >  CP009273/2715496‑2715791
                                                                                                                                                   |                                                                                                                                                    
  cccGATTTTGCCAGAAGATGAGCCACCACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAAt                                                                                                                                                  <  1:4036617/150‑1 (MQ=255)
      aTTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGAGCGGTAAATGGCg                                                                                                                                              >  2:4198537/1‑150 (MQ=255)
         ttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGttt                                                                                                                                           <  2:1627631/150‑1 (MQ=255)
         ttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGttt                                                                                                                                           >  1:2321905/1‑150 (MQ=255)
         gtGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGttt                                                                                                                                           <  2:477142/149‑1 (MQ=255)
          tGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTATg                                                                                                                                          >  1:1941968/1‑150 (MQ=255)
           gCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCAATGAAGATTTAGCCAACATGACACAGATCTACTACGAGAGGTAAATGGCGTTTGt                                                                                                                                         >  2:1171183/1‑150 (MQ=255)
            ccAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTa                                                                                                                                        <  2:1415813/150‑1 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGACTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGc                                                                                                                                      >  2:1486343/1‑150 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGGAAATGGCGTTTGTAGc                                                                                                                                      >  1:1118776/1‑150 (MQ=255)
                 agaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGt                                                                                                                                   >  1:5153076/1‑150 (MQ=255)
                   aTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTac                                                                                                                                 <  1:5073398/150‑1 (MQ=255)
                   aTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTac                                                                                                                                 <  2:1884571/150‑1 (MQ=255)
                       gCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacgacg                                                                                                                             >  2:4746488/1‑150 (MQ=255)
                       gCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacgacg                                                                                                                             >  2:1389340/1‑150 (MQ=255)
                           caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                         <  2:4619173/150‑1 (MQ=255)
                           caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                         <  2:2707006/150‑1 (MQ=255)
                              cTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAt                                                                                                                      >  1:2117327/1‑150 (MQ=255)
                                  aGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCaaa                                                                                                                  >  1:1655209/1‑150 (MQ=255)
                                     aaTTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACgg                                                                                                               >  2:2260527/1‑150 (MQ=255)
                                     aaTTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACgg                                                                                                               <  2:4533047/150‑1 (MQ=255)
                                      aTTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGa                                                                                                              >  1:2186532/1‑150 (MQ=255)
                                        tCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAag                                                                                                            >  1:2264476/1‑150 (MQ=255)
                                          aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                          >  2:4906024/1‑150 (MQ=255)
                                          aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                          >  2:2086428/1‑150 (MQ=255)
                                             tCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGTGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATc                                                                                                       >  1:3433600/1‑150 (MQ=255)
                                             tCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATc                                                                                                       <  2:4060709/150‑1 (MQ=255)
                                              cgcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATcc                                                                                                      >  1:1126670/1‑150 (MQ=255)
                                                 gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                   <  2:5028631/150‑1 (MQ=255)
                                                 gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                   >  1:2853293/1‑150 (MQ=255)
                                                  aGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCgg                                                                                                  >  2:304043/1‑150 (MQ=255)
                                                    tCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCg                                                                                                >  2:676629/1‑150 (MQ=255)
                                                    tCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCg                                                                                                >  2:1271774/1‑150 (MQ=255)
                                                     cACCAAAGAAGCTGTTTATTGCCGCTACTGTCGCGAGATCAACGAATGTACCCATGAAGAGTTGGCCAACATGACACAGATCGCCTACGATCGGGAAGTGGCGGGTGTCGCGGTACGACGGAGTGATCCGTCGGAAGAGAGCCTCGGCGt                                                                                               >  2:4020798/1‑150 (MQ=255)
                                                      aCCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTc                                                                                              <  2:702315/150‑1 (MQ=255)
                                                           agaagaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcgcg                                                                                         >  2:3864027/1‑150 (MQ=255)
                                                           agaagaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcgcg                                                                                         >  1:1125310/1‑150 (MQ=255)
                                                              agaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGc                                                                                      <  1:3322984/150‑1 (MQ=255)
                                                                 tCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAt                                                                                   <  2:3486078/150‑1 (MQ=255)
                                                                   tttATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAttt                                                                                 <  1:4946274/150‑1 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                                >  1:4604467/1‑150 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                                <  1:4100310/150‑1 (MQ=255)
                                                                     tattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTcc                                                                               <  2:2321905/150‑1 (MQ=255)
                                                                       ttaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGa                                                                             >  2:3538745/1‑150 (MQ=255)
                                                                         aCCGCTACTTTAGTGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                                           <  2:1079858/150‑1 (MQ=255)
                                                                         aCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGCTCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                                           <  2:1046921/150‑1 (MQ=255)
                                                                         aCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                                           <  1:4806085/150‑1 (MQ=255)
                                                                         aCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                                           <  2:2793211/150‑1 (MQ=255)
                                                                         aCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                                           <  2:1047106/150‑1 (MQ=255)
                                                                           cGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCt                                                                         >  1:2823042/1‑150 (MQ=255)
                                                                            gCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTg                                                                        >  1:4155601/1‑150 (MQ=255)
                                                                              tACTTGAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAt                                                                      <  2:3732064/150‑1 (MQ=255)
                                                                               aCTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGACGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATa                                                                     >  2:5124001/1‑150 (MQ=255)
                                                                                cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                    <  2:1835067/150‑1 (MQ=255)
                                                                                   tAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACAt                                                                 >  2:760375/1‑150 (MQ=255)
                                                                                    aGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATc                                                                >  2:1994631/1‑150 (MQ=255)
                                                                                      cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                              <  1:2632193/150‑1 (MQ=255)
                                                                                       gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                             >  2:360889/1‑150 (MQ=255)
                                                                                       gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                             <  2:871382/150‑1 (MQ=255)
                                                                                       gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                             <  1:2501633/150‑1 (MQ=255)
                                                                                         gaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGc                                                           <  1:4595295/150‑1 (MQ=255)
                                                                                           tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAATCGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                                         >  2:2490093/1‑150 (MQ=255)
                                                                                               cGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAtt                                                     >  1:3489641/1‑150 (MQ=255)
                                                                                                 aaTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTg                                                   >  2:454468/1‑150 (MQ=255)
                                                                                                  aTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGc                                                  <  2:5037610/150‑1 (MQ=255)
                                                                                                   tttACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCt                                                 >  2:4580324/1‑150 (MQ=255)
                                                                                                      aCCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTa                                              <  1:1844093/150‑1 (MQ=255)
                                                                                                       cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAc                                             <  1:2185959/150‑1 (MQ=255)
                                                                                                       cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAc                                             >  1:943683/1‑150 (MQ=255)
                                                                                                        ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGTGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACt                                            <  1:2406701/150‑1 (MQ=255)
                                                                                                        ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGACGAATTTGCTGTACt                                            >  1:308005/1‑150 (MQ=255)
                                                                                                            gAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                                        <  2:2020844/150‑1 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                       <  2:3805947/150‑1 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                       <  1:2355465/150‑1 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                       <  1:3005259/150‑1 (MQ=255)
                                                                                                                 tttAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTc                                   <  2:3180423/150‑1 (MQ=255)
                                                                                                                 tttAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTc                                   <  1:3761600/150‑1 (MQ=255)
                                                                                                                   tAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCgg                                 <  2:3301345/150‑1 (MQ=255)
                                                                                                                     gCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAt                               >  1:5001042/1‑150 (MQ=255)
                                                                                                                          cATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaa                          >  1:2066559/1‑150 (MQ=255)
                                                                                                                           aTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa                         >  1:4161709/1‑150 (MQ=255)
                                                                                                                            tGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                        >  2:2169160/1‑150 (MQ=255)
                                                                                                                             gACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAgg                       <  2:2181782/150‑1 (MQ=255)
                                                                                                                             gACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAgg                       <  2:2113788/150‑1 (MQ=255)
                                                                                                                               cacaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt                     <  1:1605998/150‑1 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                    >  2:966024/1‑150 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                    <  2:592159/150‑1 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                    >  2:2356543/1‑150 (MQ=255)
                                                                                                                                 caGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTa                   <  1:4294460/150‑1 (MQ=255)
                                                                                                                                 caGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTa                   >  1:78149/1‑150 (MQ=255)
                                                                                                                                  aGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAg                  >  1:4486897/1‑150 (MQ=255)
                                                                                                                                  aGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAg                  <  2:815641/150‑1 (MQ=255)
                                                                                                                                     tCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCt               >  1:852251/1‑150 (MQ=255)
                                                                                                                                       gACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTa             <  1:2739006/150‑1 (MQ=255)
                                                                                                                                       gACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTa             >  2:4260557/1‑150 (MQ=255)
                                                                                                                                        aCTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAg            >  2:4426125/1‑150 (MQ=255)
                                                                                                                                           aCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTc         <  1:1389340/150‑1 (MQ=255)
                                                                                                                                           aCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTc         <  1:4746488/150‑1 (MQ=255)
                                                                                                                                             gATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGa       <  2:4086995/150‑1 (MQ=255)
                                                                                                                                              aTCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGAc      >  1:1702174/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTT  >  CP009273/2715496‑2715791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTT  >  CP009273/2715494‑2715791
                                                                                                                                                     |                                                                                                                                                    
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                      <  SRR19973083.998560/150‑1 (MQ=60)
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                      <  SRR19973083.807293/150‑1 (MQ=60)
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                      >  SRR19973083.389133/1‑150 (MQ=60)
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                      <  SRR19973083.3611595/150‑1 (MQ=60)
    CCCGATTTTGCCAGAAGATGAGCCACCACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAAT                                                                                                                                                  <  SRR19973083.4054557/150‑1 (MQ=60)
    CCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTCCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAAT                                                                                                                                                  <  SRR19973083.4568677/150‑1 (MQ=60)
    CCCAATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAAT                                                                                                                                                  <  SRR19973083.2340427/150‑1 (MQ=60)
     CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                 >  SRR19973083.677128/1‑150 (MQ=60)
     CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                 >  SRR19973083.3427399/1‑150 (MQ=60)
           TTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTT                                                                                                                                           >  SRR19973083.2333814/1‑150 (MQ=60)
            TGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTATG                                                                                                                                          >  SRR19973083.1951889/1‑150 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                         <  SRR19973083.455659/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                         <  SRR19973083.823875/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                         <  SRR19973083.824200/150‑1 (MQ=60)
               CAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAG                                                                                                                                       >  SRR19973083.2326675/1‑150 (MQ=60)
                AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGGAAATGGCGTTTGTAGC                                                                                                                                      >  SRR19973083.1124393/1‑150 (MQ=60)
                AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGC                                                                                                                                      >  SRR19973083.1125067/1‑150 (MQ=60)
                  AAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGG                                                                                                                                    <  SRR19973083.124928/150‑1 (MQ=60)
                   AGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGT                                                                                                                                   >  SRR19973083.5174754/1‑150 (MQ=60)
                   AGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGT                                                                                                                                   >  SRR19973083.5174956/1‑150 (MQ=60)
                    GATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTA                                                                                                                                  >  SRR19973083.46357/1‑150 (MQ=60)
                     ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                 <  SRR19973083.5095101/150‑1 (MQ=60)
                      TGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACG                                                                                                                                <  SRR19973083.3724080/150‑1 (MQ=60)
                        AGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGAC                                                                                                                              >  SRR19973083.967105/1‑150 (MQ=60)
                            ACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTGCCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAT                                                                                                                          <  SRR19973083.2259955/150‑1 (MQ=60)
                              AACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTG                                                                                                                        <  SRR19973083.2632485/150‑1 (MQ=60)
                                CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                      >  SRR19973083.2128017/1‑150 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                  >  SRR19973083.1663423/1‑150 (MQ=60)
                                       AATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGG                                                                                                               <  SRR19973083.2576489/150‑1 (MQ=60)
                                        ATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGA                                                                                                              >  SRR19973083.2197479/1‑150 (MQ=60)
                                          TCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAG                                                                                                            >  SRR19973083.2276016/1‑150 (MQ=60)
                                          TCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAG                                                                                                            >  SRR19973083.2276039/1‑150 (MQ=60)
                                          TCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAG                                                                                                            >  SRR19973083.4898153/1‑150 (MQ=60)
                                               TCGCGAGTCTCCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGATTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGATATC                                                                                                       <  SRR19973083.2436683/150‑1 (MQ=60)
                                               TCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGTGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATC                                                                                                       >  SRR19973083.3449397/1‑150 (MQ=60)
                                               TCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATC                                                                                                       >  SRR19973083.1853768/1‑150 (MQ=60)
                                                CGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCC                                                                                                      >  SRR19973083.1132320/1‑150 (MQ=60)
                                                CGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCC                                                                                                      >  SRR19973083.1132277/1‑150 (MQ=60)
                                                   GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAATATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACTACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                   >  SRR19973083.2185569/1‑150 (MQ=60)
                                                   GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                   >  SRR19973083.2867365/1‑150 (MQ=60)
                                                   GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                   >  SRR19973083.2867370/1‑150 (MQ=60)
                                                        ACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTC                                                                                              >  SRR19973083.2972746/1‑150 (MQ=60)
                                                           AAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACG                                                                                           <  SRR19973083.5018711/150‑1 (MQ=60)
                                                             AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                         >  SRR19973083.1130955/1‑150 (MQ=60)
                                                             AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                         >  SRR19973083.1130980/1‑150 (MQ=60)
                                                             AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                         >  SRR19973083.3406835/1‑150 (MQ=60)
                                                                AGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGC                                                                                      <  SRR19973083.3338554/150‑1 (MQ=60)
                                                                AGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTNACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGC                                                                                      >  SRR19973083.4808420/1‑150 (MQ=60)
                                                                 GATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCG                                                                                     >  SRR19973083.1635583/1‑150 (MQ=60)
                                                                 GATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCG                                                                                     >  SRR19973083.1928123/1‑150 (MQ=60)
                                                                   TCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAT                                                                                   >  SRR19973083.1687791/1‑150 (MQ=60)
                                                                    CTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATT                                                                                  <  SRR19973083.20532/150‑1 (MQ=60)
                                                                     TTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTT                                                                                 <  SRR19973083.4967536/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                <  SRR19973083.2377437/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                >  SRR19973083.4624348/1‑150 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                <  SRR19973083.1622397/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                <  SRR19973083.4227608/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                <  SRR19973083.4118469/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGCATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                <  SRR19973083.3033967/150‑1 (MQ=60)
                                                                       TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                               <  SRR19973083.3029911/150‑1 (MQ=60)
                                                                       TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                               <  SRR19973083.2366290/150‑1 (MQ=60)
                                                                           ACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATC                                                                           <  SRR19973083.3100591/150‑1 (MQ=60)
                                                                           ACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATC                                                                           <  SRR19973083.4826653/150‑1 (MQ=60)
                                                                            CCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCC                                                                          >  SRR19973083.4105106/1‑150 (MQ=60)
                                                                            CCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCC                                                                          >  SRR19973083.1761507/1‑150 (MQ=60)
                                                                             CGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCT                                                                         >  SRR19973083.2837017/1‑150 (MQ=60)
                                                                              GCTACTTTAGCGAGATCAACGACTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                        <  SRR19973083.4946505/150‑1 (MQ=60)
                                                                              GCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                        >  SRR19973083.4173939/1‑150 (MQ=60)
                                                                              GCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                        >  SRR19973083.4173934/1‑150 (MQ=60)
                                                                              GCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                        <  SRR19973083.2509351/150‑1 (MQ=60)
                                                                                TACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAT                                                                      <  SRR19973083.2783505/150‑1 (MQ=60)
                                                                                 ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                     >  SRR19973083.1279290/1‑150 (MQ=60)
                                                                                 ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                     >  SRR19973083.481840/1‑150 (MQ=60)
                                                                                  CTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAA                                                                    >  SRR19973083.4016227/1‑150 (MQ=60)
                                                                                       GCGAGATCAACGAATTTACCCATGAAGATTTAGCAAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGAGATTTCCGATCCTGATAACATCG                                                               >  SRR19973083.4641587/1‑150 (MQ=60)
                                                                                        CGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGA                                                              <  SRR19973083.2645413/150‑1 (MQ=60)
                                                                                         ataagcacgcaggctacggATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                             >  SRR19973083.4385896/20‑150 (MQ=60)
                                                                                         ataagcacgcaggctacggATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                             >  SRR19973083.4381505/20‑150 (MQ=60)
                                                                                         GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                             <  SRR19973083.2514383/150‑1 (MQ=60)
                                                                                          AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                            >  SRR19973083.179548/1‑150 (MQ=60)
                                                                                           GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                           <  SRR19973083.4615146/150‑1 (MQ=60)
                                                                                           GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                           <  SRR19973083.4615147/150‑1 (MQ=60)
                                                                                           GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                           >  SRR19973083.3452769/1‑150 (MQ=60)
                                                                                           GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                           >  SRR19973083.2288050/1‑150 (MQ=60)
                                                                                             TCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCG                                                         <  SRR19973083.605789/150‑1 (MQ=60)
                                                                                               AACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGAATCCGATCCGTAAATGGCGTTAGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAA                                                       >  SRR19973083.4414786/1‑150 (MQ=60)
                                                                                                 CGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATT                                                     >  SRR19973083.3505610/1‑150 (MQ=60)
                                                                                                 CGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATT                                                     >  SRR19973083.3778639/1‑150 (MQ=60)
                                                                                                 CGAATTTACCCATGAAGATTTAGCCAACATGACACAGAACGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCATCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATT                                                     >  SRR19973083.4299414/1‑150 (MQ=60)
                                                                                                   AATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTG                                                   >  SRR19973083.208097/1‑150 (MQ=60)
                                                                                                        ACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTA                                              <  SRR19973083.1853392/150‑1 (MQ=60)
                                                                                                         CCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAC                                             <  SRR19973083.2197001/150‑1 (MQ=60)
                                                                                                         CCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAC                                             <  SRR19973083.2196992/150‑1 (MQ=60)
                                                                                                         CCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAC                                             <  SRR19973083.2196901/150‑1 (MQ=60)
                                                                                                         CCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAC                                             >  SRR19973083.948295/1‑150 (MQ=60)
                                                                                                          CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGTGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                            <  SRR19973083.2419060/150‑1 (MQ=60)
                                                                                                          CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGACGAATTTGCTGTACT                                            >  SRR19973083.309578/1‑150 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                       <  SRR19973083.2367601/150‑1 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                       <  SRR19973083.3019772/150‑1 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                       >  SRR19973083.1545980/1‑150 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                       >  SRR19973083.4151187/1‑150 (MQ=60)
                                                                                                                 GATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGACTTTGCTGTACTGGTTCGC                                     >  SRR19973083.2718211/1‑150 (MQ=60)
                                                                                                                 GATTTAGCCAACATGACACAGATCGACTAAGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGC                                     >  SRR19973083.946046/1‑150 (MQ=60)
                                                                                                                  ATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTcctctttcttg                                    >  SRR19973083.3365039/1‑139 (MQ=60)
                                                                                                                   TTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTC                                   <  SRR19973083.3778628/150‑1 (MQ=60)
                                                                                                                    TTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCG                                  >  SRR19973083.4136166/1‑150 (MQ=60)
                                                                                                                       GCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAT                               <  SRR19973083.5003862/150‑1 (MQ=60)
                                                                                                                       GCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAT                               >  SRR19973083.5022508/1‑150 (MQ=60)
                                                                                                                       GCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAT                               >  SRR19973083.5022555/1‑150 (MQ=60)
                                                                                                                       GCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAT                               >  SRR19973083.5022957/1‑150 (MQ=60)
                                                                                                                        CCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATC                              <  SRR19973083.2097022/150‑1 (MQ=60)
                                                                                                                            CATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAA                          >  SRR19973083.2077077/1‑150 (MQ=60)
                                                                                                                             ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                         >  SRR19973083.4180059/1‑150 (MQ=60)
                                                                                                                                 CACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGT                     <  SRR19973083.1614036/150‑1 (MQ=60)
                                                                                                                                  ACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT                    >  SRR19973083.4408057/1‑150 (MQ=60)
                                                                                                                                   CAGATCGACTACGATCGGTAAATGGCGTTTGTATCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                   <  SRR19973083.880477/150‑1 (MQ=60)
                                                                                                                                   CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                   >  SRR19973083.4097561/1‑150 (MQ=60)
                                                                                                                                   CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                   >  SRR19973083.78710/1‑150 (MQ=60)
                                                                                                                                   CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                   <  SRR19973083.4313151/150‑1 (MQ=60)
                                                                                                                                    AGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAG                  >  SRR19973083.4506383/1‑150 (MQ=60)
                                                                                                                                     GATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGG                 <  SRR19973083.2007012/150‑1 (MQ=60)
                                                                                                                                       TCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCT               >  SRR19973083.856266/1‑150 (MQ=60)
                                                                                                                                        CGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTT              <  SRR19973083.1536385/150‑1 (MQ=60)
                                                                                                                                        CGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTT              >  SRR19973083.3122717/1‑150 (MQ=60)
                                                                                                                                         GACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTA             <  SRR19973083.2752657/150‑1 (MQ=60)
                                                                                                                                         GACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTA             <  SRR19973083.2752668/150‑1 (MQ=60)
                                                                                                                                            TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT          >  SRR19973083.2769746/1‑150 (MQ=60)
                                                                                                                                             ACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTC         <  SRR19973083.4766744/150‑1 (MQ=60)
                                                                                                                                             ACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTC         <  SRR19973083.1396280/150‑1 (MQ=60)
                                                                                                                                                ATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGAC      >  SRR19973083.1710556/1‑150 (MQ=60)
                                                                                                                                                 TCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACG     <  SRR19973083.2965371/150‑1 (MQ=60)
                                                                                                                                                    GGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTT  >  SRR19973083.3591903/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTT  >  CP009273/2715494‑2715791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑