Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,715,643 G→T 62.6% E778* (GAA→TAA)  pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,715,6430GT62.6% 160.4 / inf 238E778* (GAA→TAA) pkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base T (87/62);  total (132/106)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

Only 100 of 238 total aligned reads displayed.

CCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGC  >  CP009273/2715495‑2715789
                                                                                                                                                    |                                                                                                                                                  
ccGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGa                                                                                                                                                   >  2:4307706/1‑150 (MQ=255)
  gCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGaaa                                                                                                                                                 >  1:199574/1‑150 (MQ=255)
    ccGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATg                                                                                                                                               >  1:1116542/1‑150 (MQ=255)
     cGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATgg                                                                                                                                              <  1:4077818/150‑1 (MQ=255)
      gATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGGAAATGGc                                                                                                                                             >  2:3688235/1‑150 (MQ=255)
      gATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGc                                                                                                                                             >  2:1209360/1‑150 (MQ=255)
       aTTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCg                                                                                                                                            >  2:3738648/1‑150 (MQ=255)
            gCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGt                                                                                                                                       <  1:2217311/150‑1 (MQ=255)
            gCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGt                                                                                                                                       <  1:1595886/150‑1 (MQ=255)
              cAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAg                                                                                                                                     <  2:1333591/150‑1 (MQ=255)
                 aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCgg                                                                                                                                  >  2:4460996/1‑150 (MQ=255)
                  agaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGt                                                                                                                                 <  2:1562231/150‑1 (MQ=255)
                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                       <  2:393041/150‑1 (MQ=255)
                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                       >  2:4813952/1‑150 (MQ=255)
                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                       <  2:460168/150‑1 (MQ=255)
                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                       <  1:1490975/150‑1 (MQ=255)
                                 tCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCa                                                                                                                  <  1:950633/150‑1 (MQ=255)
                                   aGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCaaa                                                                                                                >  1:1029080/1‑150 (MQ=255)
                                    gCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAAc                                                                                                               <  2:1027047/150‑1 (MQ=255)
                                    gCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAAc                                                                                                               <  2:3479762/150‑1 (MQ=255)
                                    gCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAAc                                                                                                               <  1:3679800/150‑1 (MQ=255)
                                     cAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACg                                                                                                              >  2:294229/1‑150 (MQ=255)
                                      aaTTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCACCATGACACAGATCGACTACGATCGGGACATGGCGTTTGTAGAGGTACGACGTATTGATCAAACgg                                                                                                             >  2:1873103/1‑150 (MQ=255)
                                          cATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAaga                                                                                                         >  2:681009/1‑150 (MQ=255)
                                           aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                        >  1:257953/1‑150 (MQ=255)
                                            tttCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAgaga                                                                                                       >  2:27132/1‑150 (MQ=255)
                                               cgcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATcc                                                                                                    >  1:3888527/1‑150 (MQ=255)
                                                gcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCt                                                                                                   >  2:1475840/1‑150 (MQ=255)
                                                 cgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc                                                                                                  >  2:4237633/1‑150 (MQ=255)
                                                  gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                 <  1:3473554/150‑1 (MQ=255)
                                                  gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                 <  1:3794715/150‑1 (MQ=255)
                                                  gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTATGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                 >  1:1168936/1‑150 (MQ=255)
                                                   aGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCgg                                                                                                >  1:1195304/1‑150 (MQ=255)
                                                   aGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTCTTGATCAAACGGAAGAGATCCTCgg                                                                                                >  1:2541185/1‑150 (MQ=255)
                                                    gTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGc                                                                                               <  1:3544821/150‑1 (MQ=255)
                                                    gTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGc                                                                                               <  1:4347551/150‑1 (MQ=255)
                                                    gTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGc                                                                                               <  2:74272/150‑1 (MQ=255)
                                                    gTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGc                                                                                               >  1:1005486/1‑150 (MQ=255)
                                                     tCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCg                                                                                              >  1:4771495/1‑150 (MQ=255)
                                                     tCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCg                                                                                              <  1:1385544/150‑1 (MQ=255)
                                                        ccAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCa                                                                                           >  1:1251187/1‑150 (MQ=255)
                                                        ccAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCa                                                                                           >  1:3992037/1‑150 (MQ=255)
                                                          aaaGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcg                                                                                         >  2:4750420/1‑150 (MQ=255)
                                                              aagaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTg                                                                                     <  2:3607755/150‑1 (MQ=255)
                                                                gaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCg                                                                                   >  1:3724313/1‑150 (MQ=255)
                                                                 aTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGa                                                                                  >  2:1833346/1‑150 (MQ=255)
                                                                  tCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAt                                                                                 <  2:3322772/150‑1 (MQ=255)
                                                                    tttATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAttt                                                                               >  1:3317731/1‑150 (MQ=255)
                                                                    tttATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAttt                                                                               <  2:309706/150‑1 (MQ=255)
                                                                         taCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAt                                                                          >  2:2416203/1‑150 (MQ=255)
                                                                                 cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                  >  2:2795658/1‑150 (MQ=255)
                                                                                       cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                            >  2:3246400/1‑150 (MQ=255)
                                                                                       cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                            >  2:1793896/1‑150 (MQ=255)
                                                                                        gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                           <  1:3686979/150‑1 (MQ=255)
                                                                                        gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                           >  2:2551836/1‑150 (MQ=255)
                                                                                        gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAt                                                           <  1:2916812/150‑1 (MQ=255)
                                                                                          gaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGc                                                         <  1:3849463/150‑1 (MQ=255)
                                                                                           aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                                        >  1:2806174/1‑150 (MQ=255)
                                                                                           aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                                        <  1:3673355/150‑1 (MQ=255)
                                                                                             cAACGAATTGACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGa                                                      <  2:4520432/150‑1 (MQ=255)
                                                                                                 gAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAttt                                                  <  2:1369585/150‑1 (MQ=255)
                                                                                                      tACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGt                                             <  1:1667869/150‑1 (MQ=255)
                                                                                                      tACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGt                                             >  2:1858080/1‑150 (MQ=255)
                                                                                                      tACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGt                                             >  2:1634106/1‑150 (MQ=255)
                                                                                                       aCCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTa                                            <  1:1235553/150‑1 (MQ=255)
                                                                                                       aCCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTa                                            <  2:4400405/150‑1 (MQ=255)
                                                                                                       aCCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTa                                            <  2:3903245/150‑1 (MQ=255)
                                                                                                         ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACt                                          >  1:490980/1‑150 (MQ=255)
                                                                                                         ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACt                                          <  1:1921519/150‑1 (MQ=255)
                                                                                                         ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACt                                          <  1:3113986/150‑1 (MQ=255)
                                                                                                          cATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTg                                         >  1:1791062/1‑150 (MQ=255)
                                                                                                             gAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                                      <  1:2321963/150‑1 (MQ=255)
                                                                                                             gAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                                      <  1:3760312/150‑1 (MQ=255)
                                                                                                               aGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCg                                    <  1:3048538/150‑1 (MQ=255)
                                                                                                               aGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCg                                    >  2:2377699/1‑150 (MQ=255)
                                                                                                               aGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCg                                    >  1:2308930/1‑150 (MQ=255)
                                                                                                               aGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCg                                    <  1:3049141/150‑1 (MQ=255)
                                                                                                                 aTTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCt                                  >  1:3532349/1‑150 (MQ=255)
                                                                                                                   ttAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCg                                <  2:1902356/150‑1 (MQ=255)
                                                                                                                    tAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGGAGTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCgg                               >  2:1639424/1‑150 (MQ=255)
                                                                                                                         aaCATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtctc                          >  2:2801087/1‑150 (MQ=255)
                                                                                                                          aCATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAGTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCa                         <  2:2131201/150‑1 (MQ=255)
                                                                                                                            aTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa                       >  2:1434487/1‑150 (MQ=255)
                                                                                                                             tGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                      >  1:1025465/1‑150 (MQ=255)
                                                                                                                             tGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                      >  1:1358937/1‑150 (MQ=255)
                                                                                                                             tGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                      >  1:2496351/1‑150 (MQ=255)
                                                                                                                                  caGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTa                 <  1:936415/150‑1 (MQ=255)
                                                                                                                                  caGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTa                 >  2:3492915/1‑150 (MQ=255)
                                                                                                                                    gATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAgg               <  1:1871199/150‑1 (MQ=255)
                                                                                                                                      tCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCt             <  1:1751504/150‑1 (MQ=255)
                                                                                                                                        gACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTa           <  2:842654/150‑1 (MQ=255)
                                                                                                                                         aCTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAg          <  1:3948049/150‑1 (MQ=255)
                                                                                                                                         aCTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAg          >  1:1307941/1‑150 (MQ=255)
                                                                                                                                          cTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAgg         >  2:3165748/1‑150 (MQ=255)
                                                                                                                                             cGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCg      >  2:4233317/1‑150 (MQ=255)
                                                                                                                                             cGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCg      <  2:4285803/150‑1 (MQ=255)
                                                                                                                                               aTCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGAc    <  1:4193401/150‑1 (MQ=255)
                                                                                                                                                tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACg   <  2:3770462/150‑1 (MQ=255)
                                                                                                                                                tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACg   >  1:440734/1‑150 (MQ=255)
                                                                                                                                                 cGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGc  >  2:2555507/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGC  >  CP009273/2715495‑2715789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715496‑2715792
                                                                                                                                                   |                                                                                                                                                     
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGAGCGACTACGATCGGTAA                                                                                                                                                     >  SRR19973081.6735/1‑150 (MQ=60)
 GCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAA                                                                                                                                                    >  SRR19973081.201297/1‑150 (MQ=60)
   CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                  >  SRR19973081.1123860/1‑150 (MQ=60)
    CGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGG                                                                                                                                                 <  SRR19973081.4102524/150‑1 (MQ=60)
           GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973081.1606121/150‑1 (MQ=60)
           GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973081.2231392/150‑1 (MQ=60)
             CAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAG                                                                                                                                        >  SRR19973081.386889/1‑150 (MQ=60)
              AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGC                                                                                                                                       >  SRR19973081.4120764/1‑150 (MQ=60)
               GAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCG                                                                                                                                      >  SRR19973081.3556946/1‑150 (MQ=60)
               GAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAG                                                                                                                                      >  SRR19973081.3501122/1‑150 (MQ=60)
               GAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAG                                                                                                                                      >  SRR19973081.1033974/1‑150 (MQ=60)
                   ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  <  SRR19973081.371003/150‑1 (MQ=60)
                           CAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATT                                                                                                                          <  SRR19973081.1500835/150‑1 (MQ=60)
                           CAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGCATT                                                                                                                          <  SRR19973081.1503296/150‑1 (MQ=60)
                              CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                       <  SRR19973081.4808633/150‑1 (MQ=60)
                                TCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCA                                                                                                                     <  SRR19973081.955260/150‑1 (MQ=60)
                                TCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCA                                                                                                                     <  SRR19973081.957061/150‑1 (MQ=60)
                                  AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAA                                                                                                                   >  SRR19973081.1036011/1‑150 (MQ=60)
                                   GCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAAC                                                                                                                  <  SRR19973081.3702115/150‑1 (MQ=60)
                                   GCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAAC                                                                                                                  <  SRR19973081.3702111/150‑1 (MQ=60)
                                         CATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGA                                                                                                            >  SRR19973081.4590604/1‑150 (MQ=60)
                                          ATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAG                                                                                                           >  SRR19973081.260038/1‑150 (MQ=60)
                                            TTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTTGCGAGATCAACGATTTGACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGAT                                                                                                         <  SRR19973081.3044019/150‑1 (MQ=60)
                                              CGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCC                                                                                                       >  SRR19973081.3912423/1‑150 (MQ=60)
                                                CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC                                                                                                     <  SRR19973081.4344206/150‑1 (MQ=60)
                                                CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC                                                                                                     <  SRR19973081.1876141/150‑1 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    <  SRR19973081.3496933/150‑1 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    <  SRR19973081.3818034/150‑1 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    <  SRR19973081.3494886/150‑1 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    >  SRR19973081.1177955/1‑150 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTATGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    >  SRR19973081.1176440/1‑150 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    >  SRR19973081.3832723/1‑150 (MQ=60)
                                                  AGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGG                                                                                                   >  SRR19973081.1202940/1‑150 (MQ=60)
                                                  AGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGG                                                                                                   >  SRR19973081.2557026/1‑150 (MQ=60)
                                                  AGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGG                                                                                                   >  SRR19973081.2557046/1‑150 (MQ=60)
                                                  AGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGG                                                                                                   >  SRR19973081.2557508/1‑150 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  <  SRR19973081.4373918/150‑1 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  <  SRR19973081.3566472/150‑1 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  <  SRR19973081.4390076/150‑1 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  <  SRR19973081.3484889/150‑1 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  >  SRR19973081.1012311/1‑150 (MQ=60)
                                                   GTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGC                                                                                                  >  SRR19973081.1012304/1‑150 (MQ=60)
                                                    TCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCG                                                                                                 >  SRR19973081.1229543/1‑150 (MQ=60)
                                                    TCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCG                                                                                                 >  SRR19973081.4800327/1‑150 (MQ=60)
                                                    TCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCG                                                                                                 <  SRR19973081.1394833/150‑1 (MQ=60)
                                                       CCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCA                                                                                              >  SRR19973081.1259427/1‑150 (MQ=60)
                                                       CCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCA                                                                                              >  SRR19973081.4016325/1‑150 (MQ=60)
                                                       CCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCA                                                                                              >  SRR19973081.4016335/1‑150 (MQ=60)
                                                       CCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCA                                                                                              >  SRR19973081.1259885/1‑150 (MQ=60)
                                                           AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                          >  SRR19973081.1792458/1‑150 (MQ=60)
                                                              AGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGC                                                                                       >  SRR19973081.1258693/1‑150 (MQ=60)
                                                               GATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCG                                                                                      >  SRR19973081.3747061/1‑150 (MQ=60)
                                                                  CTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATT                                                                                   <  SRR19973081.1160879/150‑1 (MQ=60)
                                                                   TTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTT                                                                                  >  SRR19973081.3338024/1‑150 (MQ=60)
                                                                    TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTC                                                                                 >  SRR19973081.3313234/1‑150 (MQ=60)
                                                                     TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                                <  SRR19973081.1367957/150‑1 (MQ=60)
                                                                     TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                                <  SRR19973081.2892659/150‑1 (MQ=60)
                                                                        TACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAT                                                                             >  SRR19973081.1941459/1‑150 (MQ=60)
                                                                            GCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                         <  SRR19973081.2141570/150‑1 (MQ=60)
                                                                              TACTTTAGCGAGATCAACTAATTTACCCATGAAGATTTAGCAAACATGACACAGATCGACTACGATCGGTAAAGGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTAACGCGTGCGATTTCCGATCCTGAT                                                                       <  SRR19973081.308786/150‑1 (MQ=60)
                                                                              TACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAT                                                                       <  SRR19973081.308809/150‑1 (MQ=60)
                                                                               ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                      <  SRR19973081.3905415/150‑1 (MQ=60)
                                                                                CTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAA                                                                     >  SRR19973081.1751771/1‑150 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.3709375/150‑1 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.3709939/150‑1 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.3709952/150‑1 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.2934797/150‑1 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.4191600/150‑1 (MQ=60)
                                                                                       GAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGAT                                                              <  SRR19973081.4077812/150‑1 (MQ=60)
                                                                                        AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                             >  SRR19973081.3793597/1‑150 (MQ=60)
                                                                                        AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                             >  SRR19973081.1314472/1‑150 (MQ=60)
                                                                                         GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                            <  SRR19973081.3873110/150‑1 (MQ=60)
                                                                                          ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           >  SRR19973081.2823247/1‑150 (MQ=60)
                                                                                          ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           <  SRR19973081.3696358/150‑1 (MQ=60)
                                                                                          ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           <  SRR19973081.3695604/150‑1 (MQ=60)
                                                                                             AACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAA                                                        >  SRR19973081.755539/1‑150 (MQ=60)
                                                                                                GAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTT                                                     <  SRR19973081.4732667/150‑1 (MQ=60)
                                                                                                     TACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGT                                                <  SRR19973081.1678768/150‑1 (MQ=60)
                                                                                                     TACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGT                                                <  SRR19973081.1678763/150‑1 (MQ=60)
                                                                                                      ACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTA                                               <  SRR19973081.1243661/150‑1 (MQ=60)
                                                                                                        CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                             >  SRR19973081.494467/1‑150 (MQ=60)
                                                                                                        CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGTCGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                             <  SRR19973081.1933349/150‑1 (MQ=60)
                                                                                                        CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                             <  SRR19973081.1933773/150‑1 (MQ=60)
                                                                                                        CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                             <  SRR19973081.3133062/150‑1 (MQ=60)
                                                                                                         CATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTG                                            >  SRR19973081.1802773/1‑150 (MQ=60)
                                                                                                           TGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGT                                          >  SRR19973081.1224595/1‑150 (MQ=60)
                                                                                                            GAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTT                                         <  SRR19973081.2336489/150‑1 (MQ=60)
                                                                                                            GAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTT                                         <  SRR19973081.3783371/150‑1 (MQ=60)
                                                                                                              AGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCG                                       <  SRR19973081.3067578/150‑1 (MQ=60)
                                                                                                              AGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCG                                       <  SRR19973081.3067305/150‑1 (MQ=60)
                                                                                                              AGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCG                                       <  SRR19973081.3067913/150‑1 (MQ=60)
                                                                                                              AGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCG                                       >  SRR19973081.2323407/1‑150 (MQ=60)
                                                                                                                ATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCT                                     >  SRR19973081.3553933/1‑150 (MQ=60)
                                                                                                                    AGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGA                                 >  SRR19973081.2324568/1‑150 (MQ=60)
                                                                                                                        AACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTC                             <  SRR19973081.18890/150‑1 (MQ=60)
                                                                                                                         ACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCA                            <  SRR19973081.27468/150‑1 (MQ=60)
                                                                                                                           ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                          >  SRR19973081.964124/1‑150 (MQ=60)
                                                                                                                           ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                          >  SRR19973081.25368/1‑150 (MQ=60)
                                                                                                                           ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                          >  SRR19973081.2361998/1‑150 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAG                         >  SRR19973081.1032386/1‑150 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAG                         >  SRR19973081.1077839/1‑150 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAG                         >  SRR19973081.1368059/1‑150 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAT‑ACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGG                        >  SRR19973081.1369851/1‑150 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAG                         >  SRR19973081.2511907/1‑150 (MQ=60)
                                                                                                                               CACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGT                      >  SRR19973081.1453161/1‑150 (MQ=60)
                                                                                                                               CACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGT                      >  SRR19973081.1453091/1‑150 (MQ=60)
                                                                                                                                ACAGATCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGGATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT                     >  SRR19973081.3928793/1‑150 (MQ=60)
                                                                                                                                 CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                    <  SRR19973081.3036707/150‑1 (MQ=60)
                                                                                                                                 CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                    <  SRR19973081.942731/150‑1 (MQ=60)
                                                                                                                                   GATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGG                  <  SRR19973081.1883264/150‑1 (MQ=60)
                                                                                                                                     TCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCT                <  SRR19973081.1762987/150‑1 (MQ=60)
                                                                                                                                     TCGACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCT                <  SRR19973081.1762927/150‑1 (MQ=60)
                                                                                                                                       GACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTA              >  SRR19973081.2499737/1‑150 (MQ=60)
                                                                                                                                       GACTACGATCGGGAAATGGCGTTTGTAGAGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTA              >  SRR19973081.1047069/1‑150 (MQ=60)
                                                                                                                                        ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAT             >  SRR19973081.3675324/1‑150 (MQ=60)
                                                                                                                                        ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             >  SRR19973081.1316683/1‑150 (MQ=60)
                                                                                                                                        ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973081.3972229/150‑1 (MQ=60)
                                                                                                                                          TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT           <  SRR19973081.2259674/150‑1 (MQ=60)
                                                                                                                                            CGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCG         <  SRR19973081.3761536/150‑1 (MQ=60)
                                                                                                                                              ATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGAC       <  SRR19973081.4219038/150‑1 (MQ=60)
                                                                                                                                              ATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGAC       <  SRR19973081.4015783/150‑1 (MQ=60)
                                                                                                                                               TCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACG      >  SRR19973081.443729/1‑150 (MQ=60)
                                                                                                                                                CGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGC     >  SRR19973081.2213547/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  <  SRR19973081.125866/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715496‑2715792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑