Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,715,643 G→T 93.2% E778* (GAA→TAA)  pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,715,6430GT93.2% 773.4 / 44.4 250E778* (GAA→TAA) pkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (3/14);  new base T (131/102);  total (134/116)
Fisher's exact test for biased strand distribution p-value = 2.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 250 total aligned reads displayed.

CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACG  >  CP009273/2715496‑2715788
                                                                                                                                                   |                                                                                                                                                 
cGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTaa                                                                                                                                                 >  1:3456999/1‑150 (MQ=255)
cGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTaa                                                                                                                                                 <  1:419662/150‑1 (MQ=255)
     gATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGc                                                                                                                                            >  1:3978154/1‑150 (MQ=255)
         ttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGttt                                                                                                                                        >  1:1583898/1‑150 (MQ=255)
           gcaagaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGt                                                                                                                                      <  1:4023281/147‑1 (MQ=255)
            ccAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTa                                                                                                                                     <  2:4682995/150‑1 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGc                                                                                                                                   >  1:3077611/1‑150 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGc                                                                                                                                   >  1:3353381/1‑150 (MQ=255)
                aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTCCGATCGGTAAATGGCGTTTGTAGCgg                                                                                                                                 >  2:3267715/1‑150 (MQ=255)
                aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCgg                                                                                                                                 <  1:522332/150‑1 (MQ=255)
                aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCgg                                                                                                                                 >  2:1475431/1‑150 (MQ=255)
                 agaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGt                                                                                                                                <  1:4353729/150‑1 (MQ=255)
                 agaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGt                                                                                                                                >  1:4515465/1‑150 (MQ=255)
                  gaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTa                                                                                                                               <  2:2878723/150‑1 (MQ=255)
                    tGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacg                                                                                                                             <  1:3177321/150‑1 (MQ=255)
                     gAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacga                                                                                                                            >  1:1662531/1‑150 (MQ=255)
                         cacaACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTa                                                                                                                        >  2:4258375/1‑150 (MQ=255)
                             aCTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGa                                                                                                                    <  2:196094/150‑1 (MQ=255)
                              cTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAt                                                                                                                   <  1:1969813/150‑1 (MQ=255)
                                 cAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCaa                                                                                                                >  1:2944468/1‑150 (MQ=255)
                                  aGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGTTTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCaaa                                                                                                               <  1:3053265/150‑1 (MQ=255)
                                  aGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCaaa                                                                                                               >  1:3667562/1‑150 (MQ=255)
                                  aGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCaaa                                                                                                               <  1:1758132/150‑1 (MQ=255)
                                    cAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACg                                                                                                             >  2:3948955/1‑150 (MQ=255)
                                    cAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACg                                                                                                             >  2:2215652/1‑150 (MQ=255)
                                      aTTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGa                                                                                                           >  2:3757793/1‑150 (MQ=255)
                                         cATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAaga                                                                                                        >  2:1779877/1‑150 (MQ=255)
                                          aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                       <  1:2969430/150‑1 (MQ=255)
                                          aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                       <  1:2003643/150‑1 (MQ=255)
                                             tCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATc                                                                                                    <  1:4740335/150‑1 (MQ=255)
                                              cgcgAGTCACCAAATAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATcc                                                                                                   <  2:4619432/150‑1 (MQ=255)
                                               gcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCt                                                                                                  >  1:2091554/1‑150 (MQ=255)
                                               gcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCt                                                                                                  <  2:402214/150‑1 (MQ=255)
                                               gcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCt                                                                                                  <  2:4619323/150‑1 (MQ=255)
                                                        cAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc                                                                                         <  1:486984/150‑1 (MQ=255)
                                                         aaaGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcg                                                                                        >  2:2792403/1‑150 (MQ=255)
                                                           agaagaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcgcg                                                                                      >  1:2630225/1‑150 (MQ=255)
                                                            gaagaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGt                                                                                     >  1:4625288/1‑150 (MQ=255)
                                                                aTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGa                                                                                 >  1:629754/1‑150 (MQ=255)
                                                                  cTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAtt                                                                               <  1:22485/150‑1 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                             >  2:1705052/1‑150 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                             >  2:2336640/1‑150 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                             >  2:3644779/1‑150 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTc                                                                             >  2:1211752/1‑150 (MQ=255)
                                                                    ttattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAGTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCACACGGAAGAGAGCCTCGGCGTCACGCGTGCGATTTc                                                                             >  1:3799319/1‑150 (MQ=255)
                                                                     tattaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTcc                                                                            <  2:142353/150‑1 (MQ=255)
                                                                      attaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCg                                                                           <  1:1069405/150‑1 (MQ=255)
                                                                       ttaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGa                                                                          >  2:2701674/1‑150 (MQ=255)
                                                                        taCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAt                                                                         <  2:2754444/150‑1 (MQ=255)
                                                                          ccGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATcc                                                                       >  1:950122/1‑150 (MQ=255)
                                                                             cTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGa                                                                    >  1:4704305/1‑150 (MQ=255)
                                                                             cTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGGATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGa                                                                    >  2:451467/1‑150 (MQ=255)
                                                                               aCTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATa                                                                  >  1:2994135/1‑150 (MQ=255)
                                                                                cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                 >  2:911694/1‑150 (MQ=255)
                                                                                cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                 >  2:2522188/1‑150 (MQ=255)
                                                                                    aGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATc                                                             >  2:3837714/1‑150 (MQ=255)
                                                                                      cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                           <  1:2510461/150‑1 (MQ=255)
                                                                                      cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                           <  1:4399591/150‑1 (MQ=255)
                                                                                        agaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATg                                                         <  1:1384334/150‑1 (MQ=255)
                                                                                          aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                                       <  1:4174905/150‑1 (MQ=255)
                                                                                           tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                                      <  1:1319656/150‑1 (MQ=255)
                                                                                           tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                                      <  2:4113563/150‑1 (MQ=255)
                                                                                           tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                                      >  2:3916101/1‑150 (MQ=255)
                                                                                            cAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGa                                                     >  1:3188913/1‑150 (MQ=255)
                                                                                                gAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCAGATAACATCGATGCCGAAtat                                                 >  2:3481715/1‑148 (MQ=255)
                                                                                                 aaTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTAGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTg                                                >  2:1124740/1‑150 (MQ=255)
                                                                                                     tACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGt                                            <  1:2938732/150‑1 (MQ=255)
                                                                                                       cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAc                                          <  2:3779375/150‑1 (MQ=255)
                                                                                                       cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAc                                          >  2:534375/1‑150 (MQ=255)
                                                                                                       cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAc                                          >  1:850085/1‑150 (MQ=255)
                                                                                                            gAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                                     <  2:2068266/150‑1 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                    <  1:2533132/150‑1 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                    >  2:3338140/1‑150 (MQ=255)
                                                                                                             aaGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                    >  2:1621274/1‑150 (MQ=255)
                                                                                                               gATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGc                                  >  2:2720008/1‑150 (MQ=255)
                                                                                                                aTTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCt                                 >  2:3542228/1‑150 (MQ=255)
                                                                                                                aTTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCt                                 >  2:379567/1‑150 (MQ=255)
                                                                                                                     gCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAt                            <  2:1971647/150‑1 (MQ=255)
                                                                                                                      ccAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtc                           <  2:1662531/150‑1 (MQ=255)
                                                                                                                         aCATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCa                        >  1:4109500/1‑150 (MQ=255)
                                                                                                                           aTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa                      <  2:3100079/150‑1 (MQ=255)
                                                                                                                            tGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                     <  2:4704787/150‑1 (MQ=255)
                                                                                                                             gACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAgg                    >  2:3492916/1‑150 (MQ=255)
                                                                                                                               cacaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt                  <  1:599364/150‑1 (MQ=255)
                                                                                                                               cacaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt                  >  2:780925/1‑150 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                 >  2:245538/1‑150 (MQ=255)
                                                                                                                                  aGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAg               >  1:2314896/1‑150 (MQ=255)
                                                                                                                                   gATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAgg              >  2:2399735/1‑150 (MQ=255)
                                                                                                                                    aTCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGc             >  1:1511486/1‑150 (MQ=255)
                                                                                                                                     tCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCt            <  1:3735367/150‑1 (MQ=255)
                                                                                                                                       gACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTa          <  2:97000/150‑1 (MQ=255)
                                                                                                                                         cTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAgg        <  2:3296012/150‑1 (MQ=255)
                                                                                                                                         cTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAgg        >  1:2682324/1‑150 (MQ=255)
                                                                                                                                          tACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGt       >  1:798783/1‑150 (MQ=255)
                                                                                                                                           aCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTAGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTc      <  1:1124740/150‑1 (MQ=255)
                                                                                                                                            cGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGGTAGGCTTAGGTCg     >  2:4244262/1‑150 (MQ=255)
                                                                                                                                             gATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGa    >  1:188194/1‑150 (MQ=255)
                                                                                                                                               tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACg  >  2:4582381/1‑150 (MQ=255)
                                                                                                                                               tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACg  <  2:3456999/150‑1 (MQ=255)
                                                                                                                                               tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACg  >  2:1022013/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACG  >  CP009273/2715496‑2715788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715494‑2715792
                                                                                                                                                     |                                                                                                                                                     
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                       <  SRR19973078.476110/150‑1 (MQ=60)
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                       <  SRR19973078.476113/150‑1 (MQ=60)
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGT                                                                                                                                                       <  SRR19973078.476008/150‑1 (MQ=60)
 CCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTA                                                                                                                                                      <  SRR19973078.257681/150‑1 (MQ=60)
 CCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTA                                                                                                                                                      <  SRR19973078.257660/150‑1 (MQ=60)
  CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAA                                                                                                                                                     >  SRR19973078.3476734/1‑150 (MQ=60)
  CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAA                                                                                                                                                     <  SRR19973078.425452/150‑1 (MQ=60)
  CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAA                                                                                                                                                     <  SRR19973078.422218/150‑1 (MQ=60)
  CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAA                                                                                                                                                     <  SRR19973078.3016649/150‑1 (MQ=60)
   GCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAA                                                                                                                                                    >  SRR19973078.1946552/1‑150 (MQ=60)
       GATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGC                                                                                                                                                >  SRR19973078.4000608/1‑150 (MQ=60)
         TTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGT                                                                                                                                              >  SRR19973078.3031091/1‑150 (MQ=60)
           TTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTT                                                                                                                                            >  SRR19973078.1593265/1‑150 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973078.4046121/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973078.327110/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973078.69990/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973078.919291/150‑1 (MQ=60)
             GCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCAAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          >  SRR19973078.4317747/1‑150 (MQ=60)
             GCAAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGT                                                                                                                                          <  SRR19973078.4046069/150‑1 (MQ=60)
                AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGC                                                                                                                                       >  SRR19973078.3095000/1‑150 (MQ=60)
                AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGC                                                                                                                                       >  SRR19973078.3372570/1‑150 (MQ=60)
                  AAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGG                                                                                                                                     <  SRR19973078.525772/150‑1 (MQ=60)
                  AAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGG                                                                                                                                     >  SRR19973078.4013909/1‑150 (MQ=60)
                   AGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGT                                                                                                                                    <  SRR19973078.4378222/150‑1 (MQ=60)
                   AGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGT                                                                                                                                    >  SRR19973078.4541025/1‑150 (MQ=60)
                     ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  <  SRR19973078.4789445/150‑1 (MQ=60)
                     ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  <  SRR19973078.490654/150‑1 (MQ=60)
                     ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  <  SRR19973078.3630115/150‑1 (MQ=60)
                      TGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACG                                                                                                                                 >  SRR19973078.604/1‑150 (MQ=60)
                      TGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACG                                                                                                                                 <  SRR19973078.3195001/150‑1 (MQ=60)
                      TGAGCCACAACTTCAGCAATTCATTTCGCGAGTCAACTAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGTATTTCCCCATGAAGATTTAGCCTACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACG                                                                                                                                 <  SRR19973078.3862430/150‑1 (MQ=60)
                       GAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGA                                                                                                                                >  SRR19973078.1672619/1‑150 (MQ=60)
                       GAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGA                                                                                                                                >  SRR19973078.1670199/1‑150 (MQ=60)
                                CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                       <  SRR19973078.1981393/150‑1 (MQ=60)
                                CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                       <  SRR19973078.1981256/150‑1 (MQ=60)
                                 TTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATC                                                                                                                      >  SRR19973078.2990230/1‑150 (MQ=60)
                                 TTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATC                                                                                                                      >  SRR19973078.2048043/1‑150 (MQ=60)
                                   CAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAA                                                                                                                    >  SRR19973078.2961273/1‑150 (MQ=60)
                                   CAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAA                                                                                                                    >  SRR19973078.2961288/1‑150 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGACGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                   <  SRR19973078.1768838/150‑1 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGTTTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                   <  SRR19973078.3070566/150‑1 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAA                                                                                                                   >  SRR19973078.3688495/1‑150 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                   >  SRR19973078.4137057/1‑150 (MQ=60)
                                    AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                   >  SRR19973078.4137009/1‑150 (MQ=60)
                                           CATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGA                                                                                                            >  SRR19973078.1817910/1‑150 (MQ=60)
                                           CATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGA                                                                                                            >  SRR19973078.1328519/1‑150 (MQ=60)
                                            ATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAG                                                                                                           <  SRR19973078.2015278/150‑1 (MQ=60)
                                            ATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAG                                                                                                           <  SRR19973078.2986375/150‑1 (MQ=60)
                                               TCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATC                                                                                                        <  SRR19973078.4766977/150‑1 (MQ=60)
                                                 GCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCT                                                                                                      >  SRR19973078.2103871/1‑150 (MQ=60)
                                                  CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACTGAAGAGATCCTC                                                                                                     >  SRR19973078.3788240/1‑150 (MQ=60)
                                                  CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC                                                                                                     >  SRR19973078.3786708/1‑150 (MQ=60)
                                                  CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC                                                                                                     <  SRR19973078.4008279/150‑1 (MQ=60)
                                                          CAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC                                                                                             <  SRR19973078.490144/150‑1 (MQ=60)
                                                          CAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC                                                                                             <  SRR19973078.490180/150‑1 (MQ=60)
                                                             AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                          >  SRR19973078.2645080/1‑150 (MQ=60)
                                                             AGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCG                                                                                          >  SRR19973078.2645246/1‑150 (MQ=60)
                                                              GAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGT                                                                                         >  SRR19973078.4651372/1‑150 (MQ=60)
                                                              GAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGT                                                                                         >  SRR19973078.4651489/1‑150 (MQ=60)
                                                                AGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGC                                                                                       <  SRR19973078.541414/150‑1 (MQ=60)
                                                                  ATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGA                                                                                     >  SRR19973078.633826/1‑150 (MQ=60)
                                                                  ATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGGATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGC                                                                                     >  SRR19973078.633825/1‑150 (MQ=60)
                                                                    CTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATT                                                                                   <  SRR19973078.22764/150‑1 (MQ=60)
                                                                    CTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATT                                                                                   <  SRR19973078.21604/150‑1 (MQ=60)
                                                                     TTGATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACTGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTT                                                                                  <  SRR19973078.4383799/150‑1 (MQ=60)
                                                                      TTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAGTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCACACGGAAGAGAGCCTCGGCGTCACGCGTGCGATTTC                                                                                 >  SRR19973078.3821132/1‑150 (MQ=60)
                                                                        ATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCG                                                                               >  SRR19973078.4425576/1‑150 (MQ=60)
                                                                        ATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCG                                                                               <  SRR19973078.1075993/150‑1 (MQ=60)
                                                                         TTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGA                                                                              >  SRR19973078.3128468/1‑150 (MQ=60)
                                                                            CCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCC                                                                           >  SRR19973078.956076/1‑150 (MQ=60)
                                                                             CGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCT                                                                          >  SRR19973078.1107331/1‑150 (MQ=60)
                                                                               CTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGA                                                                        >  SRR19973078.4730838/1‑150 (MQ=60)
                                                                               CTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGA                                                                        >  SRR19973078.4732952/1‑150 (MQ=60)
                                                                                 ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                      >  SRR19973078.3681634/1‑150 (MQ=60)
                                                                                 ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                      <  SRR19973078.4668176/150‑1 (MQ=60)
                                                                                 ACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATA                                                                      >  SRR19973078.3011210/1‑150 (MQ=60)
                                                                                    TTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACA                                                                   <  SRR19973078.2812493/150‑1 (MQ=60)
                                                                                        CGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGA                                                               <  SRR19973078.2524867/150‑1 (MQ=60)
                                                                                        CGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGA                                                               <  SRR19973078.4424235/150‑1 (MQ=60)
                                                                                          AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                             <  SRR19973078.2589440/150‑1 (MQ=60)
                                                                                          AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                             <  SRR19973078.1392942/150‑1 (MQ=60)
                                                                                          AGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATG                                                             <  SRR19973078.1393905/150‑1 (MQ=60)
                                                                                           GATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGC                                                            >  SRR19973078.309384/1‑150 (MQ=60)
                                                                                            ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           <  SRR19973078.4198732/150‑1 (MQ=60)
                                                                                             TCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCG                                                          <  SRR19973078.1733277/150‑1 (MQ=60)
                                                                                             TCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCG                                                          <  SRR19973078.1327901/150‑1 (MQ=60)
                                                                                             TCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCG                                                          <  SRR19973078.1327844/150‑1 (MQ=60)
                                                                                              CAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGA                                                         >  SRR19973078.3206619/1‑150 (MQ=60)
                                                                                              CAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGA                                                         >  SRR19973078.420479/1‑150 (MQ=60)
                                                                                                 CGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATT                                                      >  SRR19973078.841005/1‑150 (MQ=60)
                                                                                                  GTATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTT                                                     <  SRR19973078.2351645/150‑1 (MQ=60)
                                                                                                   AATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTG                                                    <  SRR19973078.3710328/150‑1 (MQ=60)
                                                                                                      TTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTG                                                 <  SRR19973078.918280/150‑1 (MQ=60)
                                                                                                       TACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGT                                                <  SRR19973078.2955566/150‑1 (MQ=60)
                                                                                                       TACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGT                                                <  SRR19973078.2955505/150‑1 (MQ=60)
                                                                                                         CCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAC                                              >  SRR19973078.855231/1‑150 (MQ=60)
                                                                                                          CCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACT                                             <  SRR19973078.2288306/150‑1 (MQ=60)
                                                                                                             TGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGT                                          >  SRR19973078.4503683/1‑150 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                        <  SRR19973078.2547677/150‑1 (MQ=60)
                                                                                                               AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                        <  SRR19973078.2547653/150‑1 (MQ=60)
                                                                                                                AGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCG                                       <  SRR19973078.1484451/150‑1 (MQ=60)
                                                                                                                   TTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTC                                    <  SRR19973078.2656490/150‑1 (MQ=60)
                                                                                                                         CAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCT                              <  SRR19973078.297832/150‑1 (MQ=60)
                                                                                                                          AACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTC                             <  SRR19973078.3372393/150‑1 (MQ=60)
                                                                                                                          AACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTC                             <  SRR19973078.2013281/150‑1 (MQ=60)
                                                                                                                           ACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCA                            >  SRR19973078.4132975/1‑150 (MQ=60)
                                                                                                                             ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                          >  SRR19973078.652586/1‑150 (MQ=60)
                                                                                                                               GACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGG                        >  SRR19973078.3205800/1‑150 (MQ=60)
                                                                                                                                 CACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGT                      >  SRR19973078.1169454/1‑150 (MQ=60)
                                                                                                                                 CACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGT                      <  SRR19973078.603290/150‑1 (MQ=60)
                                                                                                                                    AGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAG                   >  SRR19973078.2328141/1‑150 (MQ=60)
                                                                                                                                    AGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAG                   <  SRR19973078.4669209/150‑1 (MQ=60)
                                                                                                                                      ATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGC                 >  SRR19973078.1520628/1‑150 (MQ=60)
                                                                                                                                       TCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCT                <  SRR19973078.3756798/150‑1 (MQ=60)
                                                                                                                                         GACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTA              >  SRR19973078.2209210/1‑150 (MQ=60)
                                                                                                                                          ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973078.1875327/150‑1 (MQ=60)
                                                                                                                                          ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             >  SRR19973078.2374574/1‑150 (MQ=60)
                                                                                                                                           CTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGG            >  SRR19973078.2697561/1‑150 (MQ=60)
                                                                                                                                           CTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGG            >  SRR19973078.2698100/1‑150 (MQ=60)
                                                                                                                                            TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT           >  SRR19973078.1527408/1‑150 (MQ=60)
                                                                                                                                            TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT           >  SRR19973078.803415/1‑150 (MQ=60)
                                                                                                                                            TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT           >  SRR19973078.3428378/1‑150 (MQ=60)
                                                                                                                                             ACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTAGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTC          <  SRR19973078.1131537/150‑1 (MQ=60)
                                                                                                                                               GATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGA        >  SRR19973078.1655917/1‑150 (MQ=60)
                                                                                                                                               GATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGA        <  SRR19973078.3163710/150‑1 (MQ=60)
                                                                                                                                               GATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGA        >  SRR19973078.189757/1‑150 (MQ=60)
                                                                                                                                                   GGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCTCGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT    <  SRR19973078.3286241/150‑1 (MQ=60)
                                                                                                                                                   GGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT    <  SRR19973078.391475/150‑1 (MQ=60)
                                                                                                                                                   GGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT    >  SRR19973078.710526/1‑150 (MQ=60)
                                                                                                                                                     TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973078.3444546/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715494‑2715792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑