Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,715,643 G→T 91.6% E778* (GAA→TAA)  pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,715,6430GT91.6% 746.0 / 56.6 249E778* (GAA→TAA) pkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (6/15);  new base T (114/114);  total (120/129)
Fisher's exact test for biased strand distribution p-value = 6.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 250 total aligned reads displayed.

CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT  >  CP009273/2715496‑2715790
                                                                                                                                                   |                                                                                                                                                   
 gCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATAACTCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTAACCCATGAAGATTTAGCCAACAAGACACAGATCGACTACGATCGGTaaa                                                                                                                                                  >  1:683355/1‑150 (MQ=255)
   ccGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATg                                                                                                                                                <  1:1171937/150‑1 (MQ=255)
     gATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGc                                                                                                                                              <  2:1296973/150‑1 (MQ=255)
      aTTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCg                                                                                                                                             >  1:3055868/1‑150 (MQ=255)
       ttttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGt                                                                                                                                            <  2:3718288/150‑1 (MQ=255)
       ttttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGt                                                                                                                                            <  2:1060761/150‑1 (MQ=255)
        tttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGtt                                                                                                                                           <  1:434416/150‑1 (MQ=255)
        tttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGtt                                                                                                                                           <  1:1927641/150‑1 (MQ=255)
            ccAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTa                                                                                                                                       >  1:362256/1‑150 (MQ=255)
             cAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAg                                                                                                                                      >  1:3521122/1‑150 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGc                                                                                                                                     >  2:208214/1‑150 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGc                                                                                                                                     <  1:3898585/150‑1 (MQ=255)
              agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGc                                                                                                                                     <  1:2929269/150‑1 (MQ=255)
               gaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCg                                                                                                                                    <  2:2188074/150‑1 (MQ=255)
                   aTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATTACACAGATCGACTACGATCGGTAAATTGCGTTTGTATCGGTac                                                                                                                                >  2:3229990/1‑150 (MQ=255)
                      aGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacgac                                                                                                                             >  2:1723269/1‑150 (MQ=255)
                      aGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacgac                                                                                                                             >  1:1490035/1‑150 (MQ=255)
                       gCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTacgacg                                                                                                                            >  2:4135574/1‑150 (MQ=255)
                           caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTAtt                                                                                                                        <  1:2024209/150‑1 (MQ=255)
                            aaCTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACCTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTg                                                                                                                       <  2:2459639/150‑1 (MQ=255)
                            aaCTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTg                                                                                                                       <  2:285097/150‑1 (MQ=255)
                              cTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAt                                                                                                                     <  1:3135478/150‑1 (MQ=255)
                               ttCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTGAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                                                                                                                    <  2:2190279/150‑1 (MQ=255)
                                tCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCa                                                                                                                   >  1:374451/1‑150 (MQ=255)
                                   gCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAAc                                                                                                                <  1:3176621/150‑1 (MQ=255)
                                       ttCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGaa                                                                                                            >  1:750875/1‑150 (MQ=255)
                                          aTTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAagag                                                                                                         <  1:1337575/150‑1 (MQ=255)
                                           tttCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAgaga                                                                                                        <  2:75925/150‑1 (MQ=255)
                                              cgcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATcc                                                                                                     >  2:1058035/1‑150 (MQ=255)
                                               gcgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCt                                                                                                    <  2:2897733/150‑1 (MQ=255)
                                                cgAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc                                                                                                   >  1:1623231/1‑150 (MQ=255)
                                                 gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                  >  1:2626624/1‑150 (MQ=255)
                                                 gAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg                                                                                                  <  1:2428786/150‑1 (MQ=255)
                                                  aGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCgg                                                                                                 <  2:793135/150‑1 (MQ=255)
                                                     cACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGt                                                                                              <  1:3789869/150‑1 (MQ=255)
                                                     cACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGt                                                                                              <  1:1988126/150‑1 (MQ=255)
                                                        cAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc                                                                                           >  1:3160816/1‑150 (MQ=255)
                                                        cAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc                                                                                           >  2:268605/1‑150 (MQ=255)
                                                        cAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc                                                                                           >  2:2436497/1‑150 (MQ=255)
                                                        cAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc                                                                                           <  2:2705229/150‑1 (MQ=255)
                                                         aaaGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAcg                                                                                          >  1:1889329/1‑150 (MQ=255)
                                                              agaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGc                                                                                     <  2:3648597/150‑1 (MQ=255)
                                                              agaTCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGc                                                                                     >  2:2388814/1‑150 (MQ=255)
                                                                  cTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAtt                                                                                 >  2:456296/1‑150 (MQ=255)
                                                                  cTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGAtt                                                                                 >  2:456560/1‑150 (MQ=255)
                                                                      attaCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCg                                                                             >  2:1495180/1‑150 (MQ=255)
                                                                        taCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAt                                                                           <  2:3018767/150‑1 (MQ=255)
                                                                        taCCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAt                                                                           <  1:1534227/150‑1 (MQ=255)
                                                                          ccGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATcc                                                                         <  2:3030067/150‑1 (MQ=255)
                                                                           cgcgACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCt                                                                        <  2:2065699/150‑1 (MQ=255)
                                                                              tACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAt                                                                     >  2:1722446/1‑150 (MQ=255)
                                                                              tACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAt                                                                     <  1:111746/150‑1 (MQ=255)
                                                                              tACTTGAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAt                                                                     <  2:46358/150‑1 (MQ=255)
                                                                                cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                   >  2:4300497/1‑150 (MQ=255)
                                                                                cTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATaa                                                                   >  2:4240266/1‑150 (MQ=255)
                                                                                 tttAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAc                                                                  <  1:1723269/150‑1 (MQ=255)
                                                                                  ttAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACa                                                                 >  2:872999/1‑150 (MQ=255)
                                                                                   tAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACAt                                                                >  1:2718187/1‑150 (MQ=255)
                                                                                      cGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                                             >  1:3331049/1‑150 (MQ=255)
                                                                                         gaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGc                                                          >  2:2659702/1‑150 (MQ=255)
                                                                                          aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                                         >  2:1882090/1‑150 (MQ=255)
                                                                                          aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                                         >  2:4070601/1‑150 (MQ=255)
                                                                                           tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                                        <  1:1852280/150‑1 (MQ=255)
                                                                                             aaCGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGaa                                                      >  2:1581854/1‑150 (MQ=255)
                                                                                               cGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAtt                                                    <  2:820516/150‑1 (MQ=255)
                                                                                                gAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAttt                                                   >  2:1669396/1‑150 (MQ=255)
                                                                                                  aTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGc                                                 >  2:3370589/1‑150 (MQ=255)
                                                                                                    ttACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTg                                               >  2:2581615/1‑150 (MQ=255)
                                                                                                         cATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTg                                          <  2:29793/150‑1 (MQ=255)
                                                                                                          aTGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTgg                                         <  1:3501582/150‑1 (MQ=255)
                                                                                                           tGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGt                                        >  2:3516369/1‑150 (MQ=255)
                                                                                                             aaGATTTAGCCAACCTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                                      <  2:2632557/150‑1 (MQ=255)
                                                                                                              aGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCg                                     <  2:2334597/150‑1 (MQ=255)
                                                                                                                aTTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCt                                   >  1:4161506/1‑150 (MQ=255)
                                                                                                                  ttAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCg                                 >  1:993224/1‑150 (MQ=255)
                                                                                                                    aGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGa                               <  1:3595282/150‑1 (MQ=255)
                                                                                                                       cAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtct                            >  1:1954543/1‑150 (MQ=255)
                                                                                                                        aaCATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtctc                           >  1:3457739/1‑150 (MQ=255)
                                                                                                                        aaCATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtctc                           <  2:2753417/150‑1 (MQ=255)
                                                                                                                           aTGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa                        <  1:2875592/150‑1 (MQ=255)
                                                                                                                            tGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg                       <  2:1889329/150‑1 (MQ=255)
                                                                                                                              acacAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAggg                     >  2:423600/1‑150 (MQ=255)
                                                                                                                               cacaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt                    <  2:1380243/150‑1 (MQ=255)
                                                                                                                               cacaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt                    <  2:2660183/150‑1 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                   <  2:3075434/150‑1 (MQ=255)
                                                                                                                                acaGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt                   >  1:3211402/1‑150 (MQ=255)
                                                                                                                                 caGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTa                  <  2:378153/150‑1 (MQ=255)
                                                                                                                                  aGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAg                 >  2:853261/1‑150 (MQ=255)
                                                                                                                                  aGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAg                 >  1:805865/1‑150 (MQ=255)
                                                                                                                                   gATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAgg                <  2:2755427/150‑1 (MQ=255)
                                                                                                                                   gATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAgg                <  2:3032822/150‑1 (MQ=255)
                                                                                                                                    aTCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGc               >  1:409740/1‑150 (MQ=255)
                                                                                                                                    aTCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGc               >  1:2755885/1‑150 (MQ=255)
                                                                                                                                        aCTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAg           <  1:1008912/150‑1 (MQ=255)
                                                                                                                                        aCTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAg           <  1:1008948/150‑1 (MQ=255)
                                                                                                                                          tACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGt         >  1:1297087/1‑150 (MQ=255)
                                                                                                                                           aCGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTc        <  2:3570367/150‑1 (MQ=255)
                                                                                                                                            cGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCg       <  2:548289/150‑1 (MQ=255)
                                                                                                                                            cGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCg       >  2:3092318/1‑150 (MQ=255)
                                                                                                                                               tCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTTGGtagtcg    >  2:3670857/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT  >  CP009273/2715496‑2715790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715496‑2715792
                                                                                                                                                   |                                                                                                                                                     
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAA                                                                                                                                                     >  SRR19973079.3477896/1‑150 (MQ=60)
 GCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATAACTCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTAACCCATGAAGATTTAGCCAACAAGACACAGATCGACTACGATCGGTAAA                                                                                                                                                    >  SRR19973079.687103/1‑150 (MQ=60)
  CCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAAT                                                                                                                                                   <  SRR19973079.794754/150‑1 (MQ=60)
   CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                  <  SRR19973079.2830575/150‑1 (MQ=60)
   CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                  <  SRR19973079.2650152/150‑1 (MQ=60)
   CCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATG                                                                                                                                                  <  SRR19973079.1178689/150‑1 (MQ=60)
      ATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCG                                                                                                                                               >  SRR19973079.3071693/1‑150 (MQ=60)
        TTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTT                                                                                                                                             <  SRR19973079.1937975/150‑1 (MQ=60)
        TTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTT                                                                                                                                             <  SRR19973079.1937936/150‑1 (MQ=60)
        TTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTT                                                                                                                                             <  SRR19973079.436411/150‑1 (MQ=60)
        TTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTT                                                                                                                                             <  SRR19973079.437006/150‑1 (MQ=60)
          TGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTG                                                                                                                                           <  SRR19973079.1873188/150‑1 (MQ=60)
            CCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTA                                                                                                                                         <  SRR19973079.3860179/150‑1 (MQ=60)
            CCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTA                                                                                                                                         >  SRR19973079.364194/1‑150 (MQ=60)
             CAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAG                                                                                                                                        >  SRR19973079.3539205/1‑150 (MQ=60)
              AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGC                                                                                                                                       <  SRR19973079.3918365/150‑1 (MQ=60)
              AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGC                                                                                                                                       <  SRR19973079.200813/150‑1 (MQ=60)
              AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGC                                                                                                                                       <  SRR19973079.2944212/150‑1 (MQ=60)
              AGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGC                                                                                                                                       >  SRR19973079.329767/1‑150 (MQ=60)
                AAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGG                                                                                                                                     >  SRR19973079.380267/1‑150 (MQ=60)
                   ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  >  SRR19973079.1214341/1‑150 (MQ=60)
                   ATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTAC                                                                                                                                  <  SRR19973079.4049924/150‑1 (MQ=60)
                    TGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACG                                                                                                                                 <  SRR19973079.3535078/150‑1 (MQ=60)
                      AGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGAC                                                                                                                               >  SRR19973079.1498224/1‑150 (MQ=60)
                           CAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATT                                                                                                                          <  SRR19973079.2034868/150‑1 (MQ=60)
                            AACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTG                                                                                                                         <  SRR19973079.3973288/150‑1 (MQ=60)
                              CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                       <  SRR19973079.3151610/150‑1 (MQ=60)
                              CTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGAT                                                                                                                       <  SRR19973079.3151645/150‑1 (MQ=60)
                               TTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATC                                                                                                                      <  SRR19973079.4326765/150‑1 (MQ=60)
                               TTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATC                                                                                                                      <  SRR19973079.3062493/150‑1 (MQ=60)
                                TCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCA                                                                                                                     >  SRR19973079.376503/1‑150 (MQ=60)
                                TCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCA                                                                                                                     >  SRR19973079.2363405/1‑150 (MQ=60)
                                TCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCA                                                                                                                     <  SRR19973079.1333894/150‑1 (MQ=60)
                                  AGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAA                                                                                                                   >  SRR19973079.3592819/1‑150 (MQ=60)
                                   GCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAAC                                                                                                                  <  SRR19973079.3192876/150‑1 (MQ=60)
                                     AATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGG                                                                                                                >  SRR19973079.2752931/1‑150 (MQ=60)
                                       TTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACGGAA                                                                                                              >  SRR19973079.1910908/1‑150 (MQ=60)
                                       TTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAA                                                                                                              >  SRR19973079.754979/1‑150 (MQ=60)
                                       TTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAA                                                                                                              >  SRR19973079.2932553/1‑150 (MQ=60)
                                         CATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGA                                                                                                            >  SRR19973079.3785634/1‑150 (MQ=60)
                                         CATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGA                                                                                                            <  SRR19973079.1791178/150‑1 (MQ=60)
                                          ATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAG                                                                                                           <  SRR19973079.1345083/150‑1 (MQ=60)
                                                CGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC                                                                                                     >  SRR19973079.1632111/1‑150 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    <  SRR19973079.2441281/150‑1 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    >  SRR19973079.2640194/1‑150 (MQ=60)
                                                 GAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCG                                                                                                    <  SRR19973079.3394479/150‑1 (MQ=60)
                                                     CACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGT                                                                                                <  SRR19973079.3809272/150‑1 (MQ=60)
                                                     CACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGT                                                                                                <  SRR19973079.1998670/150‑1 (MQ=60)
                                                     CACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGT                                                                                                <  SRR19973079.1605172/150‑1 (MQ=60)
                                                      ACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTC                                                                                               <  SRR19973079.3065914/150‑1 (MQ=60)
                                                        CAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC                                                                                             >  SRR19973079.3177026/1‑150 (MQ=60)
                                                        CAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC                                                                                             >  SRR19973079.3176761/1‑150 (MQ=60)
                                                         AAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACG                                                                                            >  SRR19973079.1899391/1‑150 (MQ=60)
                                                          AAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGC                                                                                           >  SRR19973079.1517546/1‑150 (MQ=60)
                                                                ATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGA                                                                                     >  SRR19973079.3091354/1‑150 (MQ=60)
                                                                   TTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTT                                                                                  <  SRR19973079.1474307/150‑1 (MQ=60)
                                                                     TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                                >  SRR19973079.888777/1‑150 (MQ=60)
                                                                     TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                                >  SRR19973079.106004/1‑150 (MQ=60)
                                                                     TATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCC                                                                                >  SRR19973079.1075122/1‑150 (MQ=60)
                                                                        TACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGAT                                                                             <  SRR19973079.1542693/150‑1 (MQ=60)
                                                                          CCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCC                                                                           <  SRR19973079.3359183/150‑1 (MQ=60)
                                                                            GCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTG                                                                         >  SRR19973079.2221495/1‑150 (MQ=60)
                                                                             CTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGA                                                                        >  SRR19973079.3689432/1‑150 (MQ=60)
                                                                              TACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAT                                                                       <  SRR19973079.112695/150‑1 (MQ=60)
                                                                                 TTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAC                                                                    <  SRR19973079.1732446/150‑1 (MQ=60)
                                                                                 TTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAC                                                                    <  SRR19973079.1729112/150‑1 (MQ=60)
                                                                                   TAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACAT                                                                  >  SRR19973079.2732249/1‑150 (MQ=60)
                                                                                   TAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACAT                                                                  <  SRR19973079.737211/150‑1 (MQ=60)
                                                                                      CGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGA                                                               >  SRR19973079.3348107/1‑150 (MQ=60)
                                                                                          ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           <  SRR19973079.3640629/150‑1 (MQ=60)
                                                                                          ATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCC                                                           <  SRR19973079.3640484/150‑1 (MQ=60)
                                                                                           TCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCG                                                          <  SRR19973079.1862072/150‑1 (MQ=60)
                                                                                                GAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTT                                                     <  SRR19973079.2771943/150‑1 (MQ=60)
                                                                                                 AATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTG                                                    >  SRR19973079.1988887/1‑150 (MQ=60)
                                                                                                  ATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGC                                                   >  SRR19973079.3196802/1‑150 (MQ=60)
                                                                                                          ATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGG                                           <  SRR19973079.3519587/150‑1 (MQ=60)
                                                                                                          ATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGG                                           <  SRR19973079.3519896/150‑1 (MQ=60)
                                                                                                            GAAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTT                                         <  SRR19973079.1565630/150‑1 (MQ=60)
                                                                                                             AAGATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTC                                        <  SRR19973079.1914964/150‑1 (MQ=60)
                                                                                                                ATTTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCT                                     >  SRR19973079.4182628/1‑150 (MQ=60)
                                                                                                                  TTAGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCG                                   >  SRR19973079.998838/1‑150 (MQ=60)
                                                                                                                    AGCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGA                                 <  SRR19973079.3613553/150‑1 (MQ=60)
                                                                                                                     GCCAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAT                                <  SRR19973079.947314/150‑1 (MQ=60)
                                                                                                                       CAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCT                              >  SRR19973079.4164182/1‑150 (MQ=60)
                                                                                                                       CAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCT                              >  SRR19973079.1964955/1‑150 (MQ=60)
                                                                                                                       CAACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCT                              >  SRR19973079.1964954/1‑150 (MQ=60)
                                                                                                                        AACATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTC                             >  SRR19973079.3475588/1‑150 (MQ=60)
                                                                                                                           ATGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAA                          <  SRR19973079.2890155/150‑1 (MQ=60)
                                                                                                                            TGACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAG                         <  SRR19973079.2702794/150‑1 (MQ=60)
                                                                                                                             GACACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGG                        <  SRR19973079.628906/150‑1 (MQ=60)
                                                                                                                                ACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT                     <  SRR19973079.1261443/150‑1 (MQ=60)
                                                                                                                                ACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT                     >  SRR19973079.3227760/1‑150 (MQ=60)
                                                                                                                                ACAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT                     >  SRR19973079.3227759/1‑150 (MQ=60)
                                                                                                                                 CAGATCGACTACGATCGGTATATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTTATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAATGGTTA                    <  SRR19973079.2621200/150‑1 (MQ=60)
                                                                                                                                 CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                    <  SRR19973079.1735422/150‑1 (MQ=60)
                                                                                                                                 CAGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTA                    <  SRR19973079.3189771/150‑1 (MQ=60)
                                                                                                                                  AGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAG                   >  SRR19973079.113897/1‑150 (MQ=60)
                                                                                                                                  AGATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAG                   >  SRR19973079.810474/1‑150 (MQ=60)
                                                                                                                                   GATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGG                  >  SRR19973079.1394329/1‑150 (MQ=60)
                                                                                                                                    ATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGC                 >  SRR19973079.2770092/1‑150 (MQ=60)
                                                                                                                                    ATCGACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGC                 >  SRR19973079.412140/1‑150 (MQ=60)
                                                                                                                                        ACTACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973079.712056/150‑1 (MQ=60)
                                                                                                                                        ACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973079.1014627/150‑1 (MQ=60)
                                                                                                                                        ACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973079.1014611/150‑1 (MQ=60)
                                                                                                                                        ACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAG             <  SRR19973079.1014591/150‑1 (MQ=60)
                                                                                                                                          TACGATCGGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGT           >  SRR19973079.1304450/1‑150 (MQ=60)
                                                                                                                                                 GGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT    >  SRR19973079.1163505/1‑150 (MQ=60)
                                                                                                                                                 GGTAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCT    >  SRR19973079.1163501/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973079.1887558/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973079.3721239/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973079.3232092/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973079.3437266/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  SRR19973079.3437416/1‑150 (MQ=60)
                                                                                                                                                   TAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  <  SRR19973079.3461041/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTA  >  CP009273/2715496‑2715792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑