Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,755 T→G 21.0% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7550TG21.0% 503.7 / 70.9 296F231V (TTC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (77/152);  new base G (64/3);  total (141/155)
Fisher's exact test for biased strand distribution p-value = 2.07e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACG  >  CP009273/3080607‑3080903
                                                                                                                                                    |                                                                                                                                                     
  aTGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc                                                                                                                                                     <  2:3114881/149‑1 (MQ=255)
  aTGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc                                                                                                                                                     <  2:514272/149‑1 (MQ=255)
    gCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgt                                                                                                                                                   <  2:2039042/149‑1 (MQ=255)
     cTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgtt                                                                                                                                                  <  1:904840/149‑1 (MQ=255)
      tGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTa                                                                                                                                                 <  2:2439333/149‑1 (MQ=255)
          gagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCgg                                                                                                                                             >  2:3827464/3‑149 (MQ=255)
          gagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCgg                                                                                                                                             <  1:3907742/147‑1 (MQ=255)
             cTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTgg                                                                                                                                          >  1:1583989/1‑149 (MQ=255)
               ttCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTgggc                                                                                                                                        >  1:2004423/1‑147 (MQ=255)
                  cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCaa                                                                                                                                    >  2:3370500/1‑149 (MQ=255)
                     tCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGTGG‑CCCAAAgg                                                                                                                                 >  1:2328287/1‑149 (MQ=255)
                          aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGAc                                                                                                                            <  2:1958166/149‑1 (MQ=255)
                           ctctGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCAATGGGTGACt                                                                                                                           >  2:2276430/1‑149 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGG‑CCCCATGGGTGACAGCg                                                                                                                        >  1:1415069/1‑149 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCg                                                                                                                        <  1:2825313/149‑1 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCg                                                                                                                        <  1:2598103/149‑1 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGG‑CCCCAAGGGTGACAGCg                                                                                                                        >  1:4625049/1‑149 (MQ=255)
                              tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCTGTGG‑CCCAATGGGTGACTGCg                                                                                                                        >  1:2182010/1‑149 (MQ=255)
                               gAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCgg                                                                                                                       <  2:2892322/149‑1 (MQ=255)
                                aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCGGGGG‑GCCCAAGGGTGGcggcggg                                                                                                                      >  1:3063078/1‑148 (MQ=255)
                                 agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGATTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGG‑CCCAATGGGTGACTGCggac                                                                                                                     <  2:2945055/149‑3 (MQ=255)
                                    gaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGa                                                                                                                  >  2:2745482/1‑149 (MQ=255)
                                       cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTg                                                                                                               <  1:3627137/149‑1 (MQ=255)
                                       cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTg                                                                                                               <  1:3032656/149‑1 (MQ=255)
                                        gACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTgg                                                                                                              >  2:3336482/1‑149 (MQ=255)
                                         aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGt                                                                                                             <  1:4253805/149‑1 (MQ=255)
                                         aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGt                                                                                                             >  2:589233/1‑149 (MQ=255)
                                          ccAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTc                                                                                                            <  1:2078916/149‑1 (MQ=255)
                                            aGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGt                                                                                                          <  2:922681/149‑1 (MQ=255)
                                               aaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCCAAGGGTGACTGCGGTCAGACTGGTCGGaaa                                                                                                       >  1:1675677/1‑149 (MQ=255)
                                                ttCGCTGCAAGTAGAGGTAATGGAAGAGATAATCAAGCCAATTCTGACCTCTGTATTGCTGACTTCTGCATCCTAATTCTTCATCAACCCGACCGGTTGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGTCTGGTCGTataa                                                                                                      <  2:1575595/148‑1 (MQ=255)
                                                aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTaaaa                                                                                                      >  1:4179442/1‑149 (MQ=255)
                                                aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGG‑GCCAAAGGGTGACTGCGGTCTGTCAGGTCGTaaaa                                                                                                      >  1:782968/1‑149 (MQ=255)
                                                aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGGGG‑CCCCCTGGGGGACTGCGGTCTGACTGGTCGTgaaa                                                                                                      >  1:3600548/1‑149 (MQ=255)
                                                 tCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAt                                                                                                     <  2:711862/149‑1 (MQ=255)
                                                 tCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCCAAGGGTGACTGCGGTCTGACTGGTCGTAAAAt                                                                                                     >  1:3854355/1‑149 (MQ=255)
                                                  cGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAtt                                                                                                    <  2:4001644/149‑1 (MQ=255)
                                                   gCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTa                                                                                                   <  1:4370215/149‑1 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                  <  1:2633171/149‑1 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                  <  2:4974998/149‑1 (MQ=255)
                                                     tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATAGGGGG‑GCCAATGGGTGACTGCGGTCTGACTGGTCGTTAAAatata                                                                                                 >  1:5051846/1‑148 (MQ=255)
                                                     tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATc                                                                                                 <  1:1860484/149‑1 (MQ=255)
                                                        aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATGGGTGG‑CCCAAAGGGTGACAGCGGTCTTACTGGTGGTAAAAATATAGtt                                                                                              >  1:95480/1‑149 (MQ=255)
                                                         agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                             <  1:4956203/149‑1 (MQ=255)
                                                           aagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGAt                                                                                           <  2:4600565/149‑1 (MQ=255)
                                                           aagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGAt                                                                                           <  1:1024828/149‑1 (MQ=255)
                                                              cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAcc                                                                                        <  1:805441/149‑1 (MQ=255)
                                                               ggTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCt                                                                                       <  2:1721889/149‑1 (MQ=255)
                                                                 tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                     <  2:1558462/149‑1 (MQ=255)
                                                                     ggAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTTAAATTATCGTTGATACCTAcggcg                                                                                 >  2:2804907/1‑149 (MQ=255)
                                                                      gAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGG‑CCCAATGGGTTACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCAAcggcgg                                                                                >  2:479836/1‑149 (MQ=255)
                                                                       aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACggcggc                                                                               >  2:3324850/1‑149 (MQ=255)
                                                                       aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTAGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACggcggc                                                                               <  2:796527/149‑1 (MQ=255)
                                                                          agaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATg                                                                            <  2:5071565/149‑1 (MQ=255)
                                                                          agaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCCGTGG‑CCCCAAGGGTGTCTGCGGTCTGACGGGTCGTAAAATTATCGTTGATAACTACGGCGGCCTg                                                                            >  2:4449744/1‑149 (MQ=255)
                                                                          agaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGCTCGTGTAGTTATCCGAGG‑CCCCAAGGGTGACTGCGGTCAGACAGGTCGTAAAAATATCTATGATAACAACGGCGGCAtt                                                                            >  2:4656666/1‑148 (MQ=255)
                                                                           gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATgg                                                                           <  2:3856766/149‑1 (MQ=255)
                                                                           gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATgg                                                                           <  2:566239/149‑1 (MQ=255)
                                                                            atcatcAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGgc                                                                          <  1:4788689/149‑1 (MQ=255)
                                                                            atcatcAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGgc                                                                          >  2:4532693/1‑149 (MQ=255)
                                                                              catcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGG‑CCCCAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATAACAACCGCGGCATGgcgc                                                                        >  1:2922820/1‑149 (MQ=255)
                                                                               atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATGGGTGG‑CCCAAATGGTTACTGCGGTCTGACGGGTCGTTAAATTATCGGTGATAACTAAGGCGGCATGGcgcg                                                                       >  2:21549/1‑149 (MQ=255)
                                                                                tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCCAAGGGTGTCTGCGGTCTGACTGGTCGTAAAATTATAGTTGATAACTACGGCGGCATGGCGCGt                                                                      >  2:1426530/1‑149 (MQ=255)
                                                                                  aaGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCa                                                                    >  2:738985/1‑149 (MQ=255)
                                                                                   aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCAc                                                                   <  1:1520164/149‑1 (MQ=255)
                                                                                    gCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGG‑GCCCAAGGGGGACTGCGGGATTTCTGGTGGGTAAAATATAGTTGATAAAAAAGGCGGCATGGGGCGTCCCg                                                                  >  1:1372767/1‑149 (MQ=255)
                                                                                     ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCCCCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTTTCCGTGGCCCCAATGGGT‑AATGCGGTCTTACTTGTAGTAAAATTATCGTTTTTAACTAACGCGGCCTGGCGCGTCAccg                                                                 >  2:1861651/1‑147 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                             <  1:4976369/149‑1 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTAGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                             >  1:1965113/1‑149 (MQ=255)
                                                                                          tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCg                                                            <  2:1473344/149‑1 (MQ=255)
                                                                                                cGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCAt                                                      <  1:4522279/149‑1 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGGGG‑CCCAAGGGGTGACTGCGGTCTGACGGGTCGTAAAATTATCGTTGATACCTACGGCGGCAAGGCGCGTCACGGTGGCGGGGCATtctca                                                 >  2:2689621/1‑148 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGTGG‑CCCCAAGGGTGACTGCGGTCGGACTGGTCGTAAAAATATAGTTGTTAACAACCGCGGCATGGCGCGTCCCCGTGGCGGGGCATTctct                                                 >  1:4235124/1‑149 (MQ=255)
                                                                                                         gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAAGGCGGCATTGCGCGTCACGGTGGCGGTGCATTCTCTGGTa                                             >  2:2514073/1‑149 (MQ=255)
                                                                                                            gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGTCTGCGGTCTGACTGGTCGTAAAATTATAGTTTATTCCTACGGCGGCCTGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAg                                          >  2:4382266/1‑149 (MQ=255)
                                                                                                            gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTAACGGTGG‑CCCAATGGGTGAAGGCGGGCTGACTGGTCGTAAAATTATCGTTGATACCAACGGCGGCAGTGCGCGGCACCGTGGCGGGGCAATCACAGGTaaat                                          >  1:1273359/1‑148 (MQ=255)
                                                                                                             aCTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCCAAGGGTGACAGCGGTCTGACAGGTCGGAAAATTATAGTTGATACCAACCGCGGCAATGCGCGGCACCGGGGCCGTGCAATCTCAGGGaaata                                         >  1:3352166/1‑147 (MQ=255)
                                                                                                                 cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCa                                     <  1:3494645/149‑1 (MQ=255)
                                                                                                                  tGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCAt                                    <  1:2535192/149‑1 (MQ=255)
                                                                                                                   gCCACCAAATTCTTCCTCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATc                                   <  2:1183978/149‑1 (MQ=255)
                                                                                                                   gCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACGGCGGTCTGACTGGTCGTAAAAATATCGGTTATAACAAAGGCGGCATGGCGCGGCACGGTGGCGGTGCATTATCTGGTAAATATCCAta                                   >  2:2415420/1‑148 (MQ=255)
                                                                                                                      accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACGGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaa                                >  2:4279550/1‑149 (MQ=255)
                                                                                                                      accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGG‑CCCAAATGGTGACTGCGGTCTGACTGGTCGTAAAAATATAGTTGATACCAAAGGCGGCAAGGCGCGTCACCGTGGCGGTGCATTCTCAGGTAAAGATAAATCaaa                                >  1:1192066/1‑149 (MQ=255)
                                                                                                                      accaAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAATATACATCaaa                                >  2:1645354/1‑149 (MQ=255)
                                                                                                                          aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTg                            <  1:5150335/149‑1 (MQ=255)
                                                                                                                          aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTg                            <  1:1384452/149‑1 (MQ=255)
                                                                                                                          aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCGGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCCCGGTGGCGGGGCATTCTCTGGTAAAGATCCAAAAAAAGTg                            >  2:2070394/1‑149 (MQ=255)
                                                                                                                            ttcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGa                          <  1:4263626/149‑1 (MQ=255)
                                                                                                                             tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                         <  1:1801944/149‑1 (MQ=255)
                                                                                                                             tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                         <  2:3002764/149‑1 (MQ=255)
                                                                                                                             tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                         <  1:2510115/149‑1 (MQ=255)
                                                                                                                                 catcaACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                     <  1:856283/149‑1 (MQ=255)
                                                                                                                                 catcaACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCGGACGGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGGGGACCggt                     >  2:3263720/1‑147 (MQ=255)
                                                                                                                                  atcaACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTc                    <  2:4179442/149‑1 (MQ=255)
                                                                                                                                  atcaACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTc                    <  2:2701165/149‑1 (MQ=255)
                                                                                                                                     aaCCCGACCGGTCGTTTAGTTATAGGTGG‑CCCAAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATAACAACGGCGGCATGGCGCGTCACGGTGGCGGTGCCTTCTCTGGTAAACAAACATCAAAATTGGACCGTTcccc                 >  2:3603879/1‑147 (MQ=255)
                                                                                                                                      aCCCGACCGGTCGTTTAGGTATCGGGGG‑CCCACAGGGTGACAGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAAGGCGGCATGGCGCGGCACGGTGGCGGTGCAATCTCTGGTAAAGATACATCAAAAGTGGACCGTTCCGCa                >  2:3094819/1‑149 (MQ=255)
                                                                                                                                       cccGACCGGTCGTGTCGATATCGGTGG‑CCCAAATGGTGAATGCGGTCAGAATGGTCGTAAAAATATAGTTTATACCTACGGCGGCATGGCGCGTCACGGTGGAGGTGCATTATCTTTTAAACATCCAAAAAAAAAGGACCGTTCCCCac               >  2:106306/1‑148 (MQ=255)
                                                                                                                                           aCCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTa           <  1:1257635/149‑1 (MQ=255)
                                                                                                                                                   tttcgttATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAc   >  2:875988/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGG‑CCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACG  >  CP009273/3080607‑3080903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A