Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,755 T→G 20.5% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7550TG20.5% 437.6 / 69.7 283F231V (TTC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (85/135);  new base G (60/3);  total (145/138)
Fisher's exact test for biased strand distribution p-value = 3.06e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAC  >  CP009273/3080607‑3080902
                                                                                                                                                    |                                                                                                                                                     
 gATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgttt                                                                                                                                                      <  1:2554400/149‑1 (MQ=255)
       gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                <  2:3235456/149‑1 (MQ=255)
           agCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGt                                                                                                                                           >  2:1093234/2‑149 (MQ=255)
            gCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTg                                                                                                                                          >  1:2355177/1‑149 (MQ=255)
                 ccACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTAT‑AGGTGGcccc                                                                                                                                     >  1:1197413/1‑148 (MQ=255)
                  cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCaa                                                                                                                                    <  2:575462/149‑1 (MQ=255)
                   aCTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑t                                                                                                                                  <  2:1614046/149‑1 (MQ=255)
                   aCTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCact                                                                                                                                   >  1:4551131/1‑147 (MQ=255)
                    cTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑Tg                                                                                                                                 <  1:4529282/149‑1 (MQ=255)
                    cTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑Tg                                                                                                                                 >  2:2493118/1‑149 (MQ=255)
                      cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑Tggg                                                                                                                               <  2:4391726/149‑1 (MQ=255)
                       aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGt                                                                                                                              >  2:1939046/1‑149 (MQ=255)
                       aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGt                                                                                                                              <  1:2989083/149‑1 (MQ=255)
                       aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGt                                                                                                                              <  1:486556/149‑1 (MQ=255)
                         cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGa                                                                                                                            <  2:3736836/149‑1 (MQ=255)
                         cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGa                                                                                                                            <  2:2416738/149‑1 (MQ=255)
                          aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑AGGTGGGCCCA‑AGGGTGAc                                                                                                                           >  1:2179771/1‑149 (MQ=255)
                          aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGATCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGAc                                                                                                                           >  1:2382054/1‑149 (MQ=255)
                                aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGt                                                                                                                     >  2:1964866/1‑149 (MQ=255)
                                aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGt                                                                                                                     <  2:1676715/149‑1 (MQ=255)
                                 agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTc                                                                                                                    <  2:920323/149‑1 (MQ=255)
                                 agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTc                                                                                                                    <  1:674817/149‑1 (MQ=255)
                                 agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTc                                                                                                                    <  2:3308932/149‑1 (MQ=255)
                                 agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑GGGGTGACTGCGGTc                                                                                                                    >  2:3653758/1‑149 (MQ=255)
                                  gagaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCt                                                                                                                   <  1:1756417/149‑1 (MQ=255)
                                     aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGAc                                                                                                                <  1:3045570/149‑1 (MQ=255)
                                     aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑AGGGTGACTGCGGTCTGAc                                                                                                                >  2:2063155/1‑149 (MQ=255)
                                      tCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACt                                                                                                               <  1:1214806/149‑1 (MQ=255)
                                         aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGt                                                                                                            >  2:2423311/1‑149 (MQ=255)
                                          ccAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTc                                                                                                           <  1:3548372/149‑1 (MQ=255)
                                          ccAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTc                                                                                                           <  1:803242/149‑1 (MQ=255)
                                          ccAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAGTCGGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTc                                                                                                           <  2:2906240/149‑1 (MQ=255)
                                           cAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGGGGTCTGACTGGTCg                                                                                                          >  2:99366/1‑149 (MQ=255)
                                             gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                        >  2:3175486/1‑149 (MQ=255)
                                              aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑GGGGGGCCCAA‑TGGGTGACTGCGGTCGGACTGGTCGtga                                                                                                       >  2:1286163/1‑147 (MQ=255)
                                                   gCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGTCTGCGGTCTGACTGGTCGTAAAATTa                                                                                                  >  1:3163693/1‑149 (MQ=255)
                                                    cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                 <  2:2604670/149‑1 (MQ=255)
                                                      gCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑AGGTGGCCCAA‑TGGGTGTCTGCGGTCTGACTGGTCGTAAAATTATCg                                                                                               >  2:1202822/1‑149 (MQ=255)
                                                      gCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑TTGGTGACTGCGGTCTGACTGATCGTAAAATTATCg                                                                                               >  2:1700804/1‑149 (MQ=255)
                                                       cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGt                                                                                              <  1:3694239/149‑1 (MQ=255)
                                                         agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                            <  2:2521114/149‑1 (MQ=255)
                                                            agCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATa                                                                                         >  2:3141259/1‑149 (MQ=255)
                                                             gCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGGCCAA‑AGGGTGTCAGCGGTCTTACTGGTGGGAAAAATATAGTTtataa                                                                                        >  1:952803/1‑148 (MQ=255)
                                                                gTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATACGGT‑GCCCCA‑TGGGTGGCTGGGGTCTGACAGGGCGGAAAAATATCGGTGATaacaa                                                                                     >  1:4633518/1‑145 (MQ=255)
                                                                   aTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑AGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAAATATCGTTGATACCTAcgg                                                                                  >  2:171332/1‑149 (MQ=255)
                                                                   aTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTAT‑CGGTGGCCCCA‑AGGGTGAAGGCGGTCAGACGGGTCGTAAAATTATCGTTGATaacaaccg                                                                                  >  2:2113632/1‑142 (MQ=255)
                                                                     ggAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTAT‑CGGTGGGCCCA‑TGGGTGACTGCGGTGTGACTGGGCGTAAAAATATAGTTGTTAACTAcggcg                                                                                >  1:4171135/1‑149 (MQ=255)
                                                                      gAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCAAcggcgg                                                                               >  2:2757408/1‑149 (MQ=255)
                                                                      gAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGGGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTTACGTTGTTAACTAaggcgg                                                                               >  2:2802058/1‑149 (MQ=255)
                                                                        agagATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCCA‑TGGGTGACTGCGGTCTTACTGGTCGTAAAATTATAGTTTTTAACAAAGGCGGCa                                                                             >  1:3900420/1‑149 (MQ=255)
                                                                         gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCAt                                                                            <  1:1682152/149‑1 (MQ=255)
                                                                         gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCAt                                                                            <  2:4295982/149‑1 (MQ=255)
                                                                         gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCAt                                                                            <  1:3207012/149‑1 (MQ=255)
                                                                           gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCAACGGCGGCCTgg                                                                          >  1:1143155/1‑149 (MQ=255)
                                                                            atcatcAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGGTAT‑AGGTGGGCCAA‑TTGGTGTCGGCGGTCTGACTTGTCGTAAAAATATAGTTGATACCTACGGCGGCATTgc                                                                         >  1:1860123/1‑149 (MQ=255)
                                                                             tcatcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTAT‑AGGGGGGCCCA‑AGGGTGTCGGCGGGGTGACTGGTCGTAAAATTATAGTTGATAACTAAGGCGGCATGgcg                                                                        >  1:3004900/1‑149 (MQ=255)
                                                                                tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑AGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTAGTAAAATTATCGTTGATAACTAAGGCGGCATTGCGCGt                                                                     >  2:2956489/1‑149 (MQ=255)
                                                                                tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACCGCGGCATGGCGCGt                                                                     >  2:4491637/1‑149 (MQ=255)
                                                                                   aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCAc                                                                  >  2:3376051/1‑149 (MQ=255)
                                                                                     ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACgg                                                                <  1:4291536/149‑1 (MQ=255)
                                                                                      cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGTCTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCCTGGCGCGTCACGGt                                                               >  1:397475/1‑149 (MQ=255)
                                                                                      cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTAT‑CGGTGGCCCAA‑AGGGTTACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATAACTACGGCGGCATGGCGCGTCACGGt                                                               >  1:3504130/1‑149 (MQ=255)
                                                                                        aTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTgg                                                             <  1:3292914/149‑1 (MQ=255)
                                                                                        aTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTgg                                                             <  2:2193661/149‑1 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTAT‑AGGTGGGCCAA‑ATGGTGTCTGCGGTCTGAATGGTCGTAAAAATATCGTTGTTAACAACGGCGGCATGGCGCGTCACGGGGGc                                                            >  1:1935485/1‑149 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                            >  2:1519078/1‑149 (MQ=255)
                                                                                         ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTTT‑CGGTGGCCCCA‑TGGGTGACGGCGGGCTGACGGGTCGGAAAAATATCGTTGATACCGACGGCGGCATGGCGCGGCACGGTGGc                                                            >  2:4116822/1‑149 (MQ=255)
                                                                                            tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGt                                                         <  1:1553790/149‑1 (MQ=255)
                                                                                            tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGt                                                         <  1:4097408/149‑1 (MQ=255)
                                                                                             gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTg                                                        <  1:3594733/149‑1 (MQ=255)
                                                                                              cccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGc                                                       <  1:2002253/149‑1 (MQ=255)
                                                                                               ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                      <  1:1134948/149‑1 (MQ=255)
                                                                                               ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                      <  1:1205854/149‑1 (MQ=255)
                                                                                               ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCTAAGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                      >  2:3838907/1‑149 (MQ=255)
                                                                                                  cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtc                                                   <  1:159711/149‑1 (MQ=255)
                                                                                                    gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTAGTTAT‑CGGTGGCCCAA‑GGGGTTACTGCGGTCTGACGGGTCGTAAAATTATCGTTGATAACTACCGCGGCCTGGCGCGGCACGGTGGCGGTGCATtcac                                                 >  2:469327/1‑147 (MQ=255)
                                                                                                     aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑CGGTGGCCCAA‑AGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTctct                                                >  2:52363/1‑149 (MQ=255)
                                                                                                       tGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTgg                                              <  1:923757/149‑1 (MQ=255)
                                                                                                            gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAT‑AGGTGGCCCAA‑AGGGTGACTGCGGTCTGACTGGTCGTAAAAATATAGTTGTTAAAGAACGCGGCATGGCGCGTCAACGTGGCGGTGCATTCTCATGTaaat                                         >  1:3396400/1‑148 (MQ=255)
                                                                                                              cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGAt                                       <  1:1387960/149‑1 (MQ=255)
                                                                                                              cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGAt                                       <  1:3643640/149‑1 (MQ=255)
                                                                                                              cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑AGGTGGCCCAA‑TGGGTGACTGCGGTCTGACGGGTCGTAAAATTATAGTTGATACCTAAGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAatat                                       >  2:1380076/1‑149 (MQ=255)
                                                                                                               ttCTGCCACCAAATTCTTCATCACCCCGACCGGTCGGTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATc                                      <  2:589635/149‑1 (MQ=255)
                                                                                                                tCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATcc                                     <  2:908704/149‑1 (MQ=255)
                                                                                                                 cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCa                                    <  2:3517509/149‑1 (MQ=255)
                                                                                                                 cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCa                                    <  2:1128442/149‑1 (MQ=255)
                                                                                                                       acaAATTATTTATGAAACCGTCCGGTAGTTTTGGTTT‑AGGTGGGCCAATTGGGGGACT‑CGGTCGGTATGGGCGTTAAATTTTTCTTGTTTACTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaaa                              <  1:1102645/148‑1 (MQ=255)
                                                                                                                         aaaTTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAAGGCGGCATTGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAATATCCATCAAAAGt                            >  2:1842985/1‑149 (MQ=255)
                                                                                                                          aaTTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGGGGCCCCA‑GGGGTGACTGCGGTCTGACTGGTCGTAAAAATATCGTTGAAACCGACGGCGGCAAGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCAACAAAAGTg                           >  2:2320578/1‑149 (MQ=255)
                                                                                                                              cttcATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAcc                       <  1:1049082/149‑1 (MQ=255)
                                                                                                                                 catcaACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                    <  2:4400794/149‑1 (MQ=255)
                                                                                                                                  atcaACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTc                   >  2:1484785/1‑149 (MQ=255)
                                                                                                                                         cGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGcc            <  1:1286110/149‑1 (MQ=255)
                                                                                                                                              ggTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTACAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgc       <  2:678151/149‑1 (MQ=255)
                                                                                                                                              ggTCGTTTCGTGAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgc       <  2:4633518/149‑1 (MQ=255)
                                                                                                                                               gTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgca      >  2:3971394/1‑149 (MQ=255)
                                                                                                                                                 cgtttcgttAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagc    <  1:973853/149‑1 (MQ=255)
                                                                                                                                                  gtttcgttAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagca   >  2:3418635/1‑149 (MQ=255)
                                                                                                                                                   tttcgttAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAc  <  2:4366353/149‑1 (MQ=255)
                                                                                                                                                   tttcgttAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAc  <  1:1317213/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT‑CGGTGGCCCAA‑TGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAC  >  CP009273/3080607‑3080902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A