Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,755 T→G 27.1% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7550TG27.1% 185.8 / 49.4 158F231V (TTC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (33/80);  new base G (43/2);  total (76/82)
Fisher's exact test for biased strand distribution p-value = 1.79e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCA  >  CP009273/3080608‑3080901
                                                                                                                                                   |                                                                                                                                                   
gATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt                                                                                                                                                     <  2:2845668/148‑1 (MQ=255)
gATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt                                                                                                                                                     <  2:2845679/148‑1 (MQ=255)
  tGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc                                                                                                                                                   >  2:2787809/1‑148 (MQ=255)
    cTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgt                                                                                                                                                 <  1:3166558/148‑1 (MQ=255)
      gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttgggta                                                                                                                                               >  1:209892/1‑143 (MQ=255)
      gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTa                                                                                                                                               <  1:2148448/148‑1 (MQ=255)
       tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                              >  2:197580/1‑148 (MQ=255)
       tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACACGACCGGTCGTGGCGTTAt                                                                                                                                              >  1:738543/1‑148 (MQ=255)
        cgagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTAta                                                                                                                                             >  1:854044/4‑147 (MQ=255)
        cgagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAta                                                                                                                                             >  1:854036/4‑147 (MQ=255)
           gCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGt                                                                                                                                          >  2:858424/1‑148 (MQ=255)
            cTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCgggg                                                                                                                                         >  1:483657/1‑146 (MQ=255)
               tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTgggc                                                                                                                                      >  1:55592/1‑146 (MQ=255)
               tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGATCGTTTCGTTATCGGTGGcc                                                                                                                                      >  2:2592593/1‑148 (MQ=255)
                ccACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                     >  2:3198208/1‑148 (MQ=255)
                     cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATgg                                                                                                                                <  1:1073141/148‑1 (MQ=255)
                       gCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGt                                                                                                                              <  2:3420694/148‑1 (MQ=255)
                       gCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGt                                                                                                                              <  2:3420513/148‑1 (MQ=255)
                        cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAAAGGGTg                                                                                                                             >  2:3072163/1‑148 (MQ=255)
                        cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTAGTTATCGGTGGCCCAATGGGTg                                                                                                                             >  2:1152906/1‑148 (MQ=255)
                         aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAACTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGa                                                                                                                            <  2:1542207/148‑1 (MQ=255)
                          ctctGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGAc                                                                                                                           <  1:1488241/148‑1 (MQ=255)
                               aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTGGGTTATAGGTGGCCCAAAGGGTGAcggggg                                                                                                                      >  1:756312/1‑143 (MQ=255)
                                agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                     <  2:813046/148‑1 (MQ=255)
                                agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                     >  2:1415145/1‑148 (MQ=255)
                                agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                     <  2:1379882/148‑1 (MQ=255)
                                agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                     >  2:1124674/1‑148 (MQ=255)
                                agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                     <  2:2256694/148‑1 (MQ=255)
                                    aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAGTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCAATGGGTGACTGCGGTCTGa                                                                                                                 <  1:1691929/148‑1 (MQ=255)
                                     tCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGGCCCAATGGGTGACTGCGGTCTgtc                                                                                                                >  2:930572/1‑146 (MQ=255)
                                      cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACt                                                                                                               <  2:1083884/148‑1 (MQ=255)
                                          cAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑c                                                                                                          >  2:3547805/1‑148 (MQ=255)
                                            gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGt                                                                                                        <  1:3225735/148‑1 (MQ=255)
                                            gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGt                                                                                                        <  1:3225776/148‑1 (MQ=255)
                                             aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATCGGTGGCCCAAGGGGTGGCTGCGGTCTGACTGGT‑Cgga                                                                                                       >  1:2609954/1‑146 (MQ=255)
                                             aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCGGGGGCCCCATGGGTGACTGCGGTCGGACGGGG‑CGTa                                                                                                       >  1:1177346/1‑148 (MQ=255)
                                             aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCGGGGGCCCAATGGGTGCCGGCGGTCTGACTGGT‑CGTa                                                                                                       >  1:2610005/1‑148 (MQ=255)
                                             aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGGGGCCCACTGGGTGACTGCGGTCTGACTGGT‑CGTa                                                                                                       <  1:1344175/148‑1 (MQ=255)
                                              aaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTaa                                                                                                      <  2:2399927/148‑1 (MQ=255)
                                               aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGGCCCATGGGTGTCGGCGGTCTGGCTGGT‑GGTcaa                                                                                                     >  1:2286656/1‑148 (MQ=255)
                                               aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAATCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATGGGTGAGTGCGGTCTGACTGGT‑CGTaaa                                                                                                     >  2:1329434/1‑148 (MQ=255)
                                                tCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGGCCCCAAGGGTGTCTGCGGTCTTACGGGT‑GGTaaaa                                                                                                    >  1:3478852/1‑148 (MQ=255)
                                                    tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTAt                                                                                                <  2:122197/148‑1 (MQ=255)
                                                     gCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATc                                                                                               <  2:2971731/148‑1 (MQ=255)
                                                     gCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGCCCAAAGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATc                                                                                               >  1:2307466/1‑148 (MQ=255)
                                                      cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGGGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTAtag                                                                                              >  1:2908973/1‑146 (MQ=255)
                                                         gaagCGGTAATGGAAGAGATCATCAATCCATTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCTACCGGTAGTTTGGTTATCGGTGGCCCAATGGGTGACTGCGGTCGGACTGGT‑CGTAAAATTATCGTTg                                                                                           <  2:384692/148‑1 (MQ=255)
                                                         gaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTg                                                                                           <  2:384203/148‑1 (MQ=255)
                                                         gaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTTTAGGTGGGCCCAAGGGGGTCTGAGGGGTGGCTGGTGGGTAAAAtatatttt                                                                                            >  1:1221007/1‑141 (MQ=255)
                                                          aagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGa                                                                                          <  1:27805/148‑1 (MQ=255)
                                                          aagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGa                                                                                          <  2:3436941/148‑1 (MQ=255)
                                                             cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGGCCAAATGGTGTCTGCGGGCTGGCTGGT‑CGTTAAATTATCGTTGATAc                                                                                       >  2:916268/1‑148 (MQ=255)
                                                             cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCCATGGGTTACTGCGGTCGGACTGGG‑CGGAAAATAATCGTTGATaa                                                                                       >  2:916191/1‑147 (MQ=255)
                                                              ggTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATAcc                                                                                      <  2:3145616/148‑1 (MQ=255)
                                                              ggTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGGGGGCCAAAGGGGGCATGCCGTAGGTCTGGT‑CGGAAAAATATAGGTGATAcc                                                                                      >  1:1683811/1‑148 (MQ=255)
                                                                 aaTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTTTAGGGGGCCCAAAGGGTGGCTGCGGGCTGACGGGT‑CGTAAAAATATCTTTGTTACCTaa                                                                                   >  1:3048400/1‑147 (MQ=255)
                                                                    ggAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTAcggc                                                                                <  1:1008192/148‑1 (MQ=255)
                                                                    ggAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTAcggc                                                                                <  1:2785057/148‑1 (MQ=255)
                                                                        gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTTTAGGTGGCCCAAAGGGTGACAGCGGTCTGACTGGT‑CGTGAAATTATAGTTGTTAACAACGGCGGCa                                                                            >  1:2448958/1‑148 (MQ=255)
                                                                            tcatcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATGGGGGGGGCCATGGGTGTCTGCGGGCTGAGTGGG‑GGTGAAATTATAGTTGATAACTACCGCGGCCTGgc                                                                        >  1:803948/1‑148 (MQ=255)
                                                                            tcatcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCCATGGGTGTCTGCGGTCTGACTGGT‑CGTAAAATTATAGTTGTTACCTACGGCGGCCTGgc                                                                        >  2:947107/1‑148 (MQ=255)
                                                                             catcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGgcg                                                                       >  2:1926371/1‑148 (MQ=255)
                                                                             catcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGGCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGgcg                                                                       >  2:498864/1‑148 (MQ=255)
                                                                              atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATGGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTTAAATTATCGTTGTTACCTACGGCGGCCTGgggc                                                                      >  2:769850/1‑148 (MQ=255)
                                                                               tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGGCCCAATGGGTGACTGCGGTCTTCGTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGcgcg                                                                     >  2:578131/1‑148 (MQ=255)
                                                                               tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGcgcg                                                                     <  2:862608/148‑1 (MQ=255)
                                                                                  aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCa                                                                  >  2:1483866/1‑148 (MQ=255)
                                                                                     cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACgg                                                               <  1:64784/148‑1 (MQ=255)
                                                                                     cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACgg                                                               <  1:64730/148‑1 (MQ=255)
                                                                                        ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGGTGGGCCCAAGGGTGTCTTGGGGCTGACTGGT‑GGTAAAAATATAGTTGTTACAAAAGGCGGCCGGGGGCGGCAccgggg                                                            >  1:1251603/1‑143 (MQ=255)
                                                                                        ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGGTGGGCCCAAGGGTGGCTGCGGGGTGTCTGGT‑GGGAAAATTATATTTTTTACAAAAGGCGGCATTGGGGGTCACGGTgg                                                            >  1:710446/1‑148 (MQ=255)
                                                                                         tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTTTCGGGGGGCCCATGGGTGTCGGCGGTGTGACTGGT‑CGGAAAAATATCGTTGTTAACAACCGCGGCCTGGCGCGTCACGGTGGc                                                           >  1:2466182/1‑148 (MQ=255)
                                                                                          cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCg                                                          <  1:279766/148‑1 (MQ=255)
                                                                                          cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGATCGTTTCGTTATCGGTGGCCCAATGCGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCg                                                          <  1:2592593/148‑1 (MQ=255)
                                                                                                 cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCAtt                                                   <  2:413595/148‑1 (MQ=255)
                                                                                                  tGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtc                                                  <  2:1666588/148‑1 (MQ=255)
                                                                                                   gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCCATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATAGTTGATACCAACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtct                                                 >  2:873562/1‑148 (MQ=255)
                                                                                                    aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtctc                                                <  1:1808304/148‑1 (MQ=255)
                                                                                                       ggCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATAGGTGGGCCCAAGGGTTACTGGGGTATGAGTGGT‑GGTTAAAATATAGTTGATTACTACGGCGGCATGGCGCGTCACCGTGGCGGGGGAttgtattg                                             >  1:2737965/1‑142 (MQ=255)
                                                                                                          tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaa                                          <  1:262221/148‑1 (MQ=255)
                                                                                                            aCTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTATCTGGTAAAg                                        >  2:2305452/1‑148 (MQ=255)
                                                                                                             cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGa                                       <  2:2913559/148‑1 (MQ=255)
                                                                                                             cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGa                                       <  2:1207905/148‑1 (MQ=255)
                                                                                                             cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGa                                       <  1:1907556/148‑1 (MQ=255)
                                                                                                             cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAAAGGGTGACAGCGGTCTGACTGGT‑CGTAAAATTATAGTTGATAACAAAGGCGGCATGGCGCGTCACGGTGGCGGTGCCTTATCATGTaaaca                                       >  1:3526452/1‑146 (MQ=255)
                                                                                                              ttCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGAt                                      <  1:3547805/148‑1 (MQ=255)
                                                                                                               tCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATc                                     <  1:1530278/148‑1 (MQ=255)
                                                                                                                      ccaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaa                              <  1:1666836/148‑1 (MQ=255)
                                                                                                                        aaaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAg                            <  2:1150857/148‑1 (MQ=255)
                                                                                                                          aTTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACGGGT‑CGTTAAATTTTCGTTGATAACAACGGCGGCATGGCGCGTCACGGTGGGGGTGCATTCTCTGGTAAAGATCCATCAAAAGTg                          >  2:2809591/1‑148 (MQ=255)
                                                                                                                             cttcATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATAACTACCGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                       >  2:2258350/1‑148 (MQ=255)
                                                                                                                                 atcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                   <  2:203316/148‑1 (MQ=255)
                                                                                                                                 atcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                   <  2:203427/148‑1 (MQ=255)
                                                                                                                                 atcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                   <  2:785562/148‑1 (MQ=255)
                                                                                                                                        cGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGc            <  1:1124674/148‑1 (MQ=255)
                                                                                                                                             ggTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACg       <  2:854036/148‑1 (MQ=255)
                                                                                                                                                  tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagca  >  2:176378/1‑148 (MQ=255)
                                                                                                                                                  tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagca  >  1:1845841/1‑148 (MQ=255)
                                                                                                                                                  tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagca  >  2:1013997/1‑148 (MQ=255)
                                                                                                                                                  tttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcagca  >  1:1845728/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGT‑CGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCA  >  CP009273/3080608‑3080901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A