Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 482,757 G→T 88.2% A256S (GCG→TCG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273482,7570GT88.2% 391.5 / 55.4 170A256S (GCG→TCG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base G (8/12);  new base T (74/76);  total (82/88)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 173 total aligned reads displayed.

GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGC  >  CP009273/482612‑482902
                                                                                                                                                 |                                                                                                                                                 
    gCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTaa                                                                                                                                             >  2:3062369/1‑148 (MQ=255)
      gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAAc                                                                                                                                           <  2:2078930/148‑1 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                         >  2:1999533/1‑148 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                         >  2:631631/1‑148 (MQ=255)
             aaTGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgc                                                                                                                                    >  2:1494879/1‑148 (MQ=255)
               tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgcgc                                                                                                                                  <  2:420325/148‑1 (MQ=255)
               tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgcgc                                                                                                                                  <  1:118963/148‑1 (MQ=255)
               tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgcgc                                                                                                                                  <  2:2889276/148‑1 (MQ=255)
               tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAgcgc                                                                                                                                  <  2:3192394/148‑1 (MQ=255)
               tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAgcgc                                                                                                                                  <  2:3192393/148‑1 (MQ=255)
                gCGGAGATTGACCAGGAGGGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc                                                                                                                                 <  2:2704236/148‑1 (MQ=255)
                gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc                                                                                                                                 <  2:1664263/148‑1 (MQ=255)
                gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc                                                                                                                                 <  2:2704368/148‑1 (MQ=255)
                    agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc                                                                                                                             >  2:813302/1‑148 (MQ=255)
                    agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc                                                                                                                             >  1:1047096/1‑148 (MQ=255)
                     gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACt                                                                                                                            >  1:1658306/1‑148 (MQ=255)
                     gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACt                                                                                                                            >  1:1660238/1‑148 (MQ=255)
                      aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACtt                                                                                                                           <  1:152307/148‑1 (MQ=255)
                                 gcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaa                                                                                                                <  1:1077674/148‑1 (MQ=255)
                                  cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa                                                                                                               <  2:578268/148‑1 (MQ=255)
                                   gTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAAc                                                                                                              <  2:450433/148‑1 (MQ=255)
                                   gTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAAc                                                                                                              <  2:945571/148‑1 (MQ=255)
                                    tAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACg                                                                                                             <  2:3126925/148‑1 (MQ=255)
                                    tAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACg                                                                                                             >  1:1330768/1‑148 (MQ=255)
                                       aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcg                                                                                                          >  1:1078317/1‑148 (MQ=255)
                                        aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcgc                                                                                                         <  1:1931642/148‑1 (MQ=255)
                                        aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcgc                                                                                                         >  1:1314592/1‑148 (MQ=255)
                                          ccTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAg                                                                                                       >  2:2293727/1‑148 (MQ=255)
                                            tgtaaGGGAACACCGTCTTGCTGTATACCTTGAAAAAGCAACGTGATTCGGTGACGGCGAACAGCGCTCTTCTGGAGCACCAGTTACAACTGTTGAAATAATCGTTAAACAGAAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGa                                                                                                     <  1:2509425/145‑1 (MQ=255)
                                             ggAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGaa                                                                                                    >  2:1490892/1‑148 (MQ=255)
                                             ggAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGAAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGaa                                                                                                    >  2:2643384/1‑148 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCACCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCTGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                   <  1:2920066/148‑1 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                   >  1:2202511/1‑148 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                   >  1:1840749/1‑148 (MQ=255)
                                                    aaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtct                                                                                             >  2:548727/1‑148 (MQ=255)
                                                    aaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtct                                                                                             >  1:1730433/1‑148 (MQ=255)
                                                          ttgttGCAGTATACCTTGAAAAAGCAACGTTTTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGTTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCgg                                                                                       <  1:957747/145‑1 (MQ=255)
                                                          gTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCgg                                                                                       <  2:3220047/148‑1 (MQ=255)
                                                          gTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCgg                                                                                       <  1:3296089/148‑1 (MQ=255)
                                                                  ggATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCTGATGAAGcc                                                                               >  2:3286681/1‑148 (MQ=255)
                                                                  ggATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGcc                                                                               >  1:1484108/1‑148 (MQ=255)
                                                                    aTACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgc                                                                             >  1:1999721/1‑148 (MQ=255)
                                                                     tACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcg                                                                            >  1:1347090/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                           >  1:702464/1‑148 (MQ=255)
                                                                        cTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGt                                                                         >  2:1168800/1‑148 (MQ=255)
                                                                         ttGCAAAAGCAACGTGATAACGGGACGGCGAACAGCCCTCGGCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAATTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTa                                                                        <  1:1899013/148‑1 (MQ=255)
                                                                           gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt                                                                      <  2:2414286/148‑1 (MQ=255)
                                                                           gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt                                                                      <  2:1260350/148‑1 (MQ=255)
                                                                           gCAAAAGCAACGCGAGTACGTGCCGGCGAACAGCGCTCGTCGGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAACCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt                                                                      <  2:1799161/148‑1 (MQ=255)
                                                                            cAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTc                                                                     <  1:2126538/148‑1 (MQ=255)
                                                                                aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc                                                                 >  2:327892/1‑148 (MQ=255)
                                                                                aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc                                                                 <  2:1328211/148‑1 (MQ=255)
                                                                                 gCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCt                                                                >  1:756766/1‑148 (MQ=255)
                                                                                 gCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCt                                                                >  2:682978/1‑148 (MQ=255)
                                                                                   aaCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTaa                                                              <  2:2924616/148‑1 (MQ=255)
                                                                                    aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt                                                             <  2:2377875/148‑1 (MQ=255)
                                                                                    aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGTCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt                                                             <  2:3232347/148‑1 (MQ=255)
                                                                                    aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt                                                             <  1:2636830/148‑1 (MQ=255)
                                                                                       tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCg                                                          >  1:1235255/1‑148 (MQ=255)
                                                                                        gATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGc                                                         <  1:631631/148‑1 (MQ=255)
                                                                                        gATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGc                                                         >  1:1835981/1‑148 (MQ=255)
                                                                                          ttACGTGACGGCGAACTGCGCTGGTCTGGATCACCTGTTACAACTTTTGCAATAATCGGTAAACCGCAAGCGACTCACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                       <  1:2393542/148‑1 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                       >  2:395317/1‑148 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                       >  1:2526556/1‑148 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                       <  1:813302/148‑1 (MQ=255)
                                                                                           tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg                                                      >  1:513829/1‑148 (MQ=255)
                                                                                           tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg                                                      >  1:1212352/1‑148 (MQ=255)
                                                                                            aCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt                                                     <  2:1469485/148‑1 (MQ=255)
                                                                                            aCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt                                                     <  2:3003312/148‑1 (MQ=255)
                                                                                            aCGTGACGGCGAACAGCGCTCGTCGGGAGCACCTGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt                                                     <  1:2787457/148‑1 (MQ=255)
                                                                                                gACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAg                                                 >  2:1124561/1‑148 (MQ=255)
                                                                                                 aCGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGc                                                <  2:2768161/148‑1 (MQ=255)
                                                                                                    gCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg                                             <  2:1078012/148‑1 (MQ=255)
                                                                                                        aCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACt                                         >  1:766294/1‑148 (MQ=255)
                                                                                                        aCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACt                                         >  1:318490/1‑148 (MQ=255)
                                                                                                        aCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACt                                         >  1:766335/1‑148 (MQ=255)
                                                                                                        aCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACt                                         >  1:2580825/1‑148 (MQ=255)
                                                                                                         cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg                                        <  1:412790/148‑1 (MQ=255)
                                                                                                            cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                     <  1:1733647/148‑1 (MQ=255)
                                                                                                            cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                     >  1:889843/1‑148 (MQ=255)
                                                                                                             gcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaaa                                    <  1:1490451/148‑1 (MQ=255)
                                                                                                              cTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAt                                   <  2:1467649/148‑1 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                               >  2:2425490/1‑148 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                               >  1:742644/1‑148 (MQ=255)
                                                                                                                   cTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc                              <  1:3221360/148‑1 (MQ=255)
                                                                                                                      gAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagc                           <  2:2555714/148‑1 (MQ=255)
                                                                                                                         cACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCACCGGATGAATCAGCGCGTATTCAGGCTAATACGCTGGTGAAGCAGGAACTGGAAATTAAACAGCAGt                        >  1:368474/1‑148 (MQ=255)
                                                                                                                           ccAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa                      <  1:2178959/148‑1 (MQ=255)
                                                                                                                              gTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGt                   <  1:2311100/148‑1 (MQ=255)
                                                                                                                               ttACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTc                  <  2:1342383/148‑1 (MQ=255)
                                                                                                                               ttACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTc                  <  2:1658306/148‑1 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt             >  1:1198982/1‑148 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt             >  1:656667/1‑148 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt             <  2:2179851/148‑1 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt             >  1:1305671/1‑148 (MQ=255)
                                                                                                                                        ttGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGa         <  2:1844329/148‑1 (MQ=255)
                                                                                                                                          gCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGAtt       <  1:1910279/148‑1 (MQ=255)
                                                                                                                                             agaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAcc    >  1:2824521/1‑148 (MQ=255)
                                                                                                                                             agaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAcc    >  1:2732381/1‑148 (MQ=255)
                                                                                                                                               aagCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcgc  >  1:979159/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGC  >  CP009273/482612‑482902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCG  >  CP009273/482609‑482903
                                                                                                                                                    |                                                                                                                                                  
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATC                                                                                                                                                   <  SRR19973070.608637/150‑1 (MQ=60)
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATC                                                                                                                                                   <  SRR19973070.1685802/150‑1 (MQ=60)
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATC                                                                                                                                                   <  SRR19973070.1685778/150‑1 (MQ=60)
 CAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCG                                                                                                                                                  >  SRR19973070.724384/1‑150 (MQ=60)
  AGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGG                                                                                                                                                 >  SRR19973070.1085515/1‑150 (MQ=60)
     AGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAA                                                                                                                                              <  SRR19973070.3021226/150‑1 (MQ=60)
                  TGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCT                                                                                                                                 <  SRR19973070.120032/150‑1 (MQ=60)
                     GGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAC                                                                                                                              >  SRR19973070.1054472/1‑150 (MQ=60)
                      GAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCTGTAAACAGCAAGCGCCTGACT                                                                                                                             >  SRR19973070.1671666/1‑150 (MQ=60)
                      GAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACT                                                                                                                             >  SRR19973070.1669729/1‑150 (MQ=60)
                        GATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTT                                                                                                                           <  SRR19973070.1929353/150‑1 (MQ=60)
                         ATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTA                                                                                                                          <  SRR19973070.153585/150‑1 (MQ=60)
                               CAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATAACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAA                                                                                                                    >  SRR19973070.1220268/1‑150 (MQ=60)
                                   AGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAA                                                                                                                >  SRR19973070.369466/1‑150 (MQ=60)
                                    GCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAAC                                                                                                               <  SRR19973070.1085177/150‑1 (MQ=60)
                                     CGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACG                                                                                                              >  SRR19973070.1339945/1‑150 (MQ=60)
                                     CGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACG                                                                                                              >  SRR19973070.2142005/1‑150 (MQ=60)
                                        AAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCG                                                                                                           >  SRR19973070.1085820/1‑150 (MQ=60)
                                         AAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGC                                                                                                          >  SRR19973070.1323705/1‑150 (MQ=60)
                                         AAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGC                                                                                                          >  SRR19973070.1323937/1‑150 (MQ=60)
                                          AAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCA                                                                                                         >  SRR19973070.1419086/1‑150 (MQ=60)
                                          AAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCA                                                                                                         >  SRR19973070.2401176/1‑150 (MQ=60)
                                           AGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAG                                                                                                        <  SRR19973070.1944645/150‑1 (MQ=60)
                                               TGTAAGGGAACACCGTCTTGCTGTATACCTTGAAAAAGCAACGTGATTCGGTGACGGCGAACAGCGCTCTTCTGGAGCACCAGTTACAACTGTTGAAATAATCGTTAAACAGAAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                    <  SRR19973070.2525751/150‑1 (MQ=60)
                                               TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                    >  SRR19973070.2217022/1‑150 (MQ=60)
                                               TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                    >  SRR19973070.1853396/1‑150 (MQ=60)
                                               TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                    >  SRR19973070.1853384/1‑150 (MQ=60)
                                                 tatggtAACACCGTGTTGCAGGATACTTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTGTGGAGCACCATTTACAACTGTTGCATGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                  <  SRR19973070.1632611/144‑1 (MQ=60)
                                                 GAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCACCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCTGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                  <  SRR19973070.2938922/150‑1 (MQ=60)
                                                     GGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                              >  SRR19973070.1742436/1‑150 (MQ=60)
                                                             ttgTTGCAGTATACCTTGAAAAAGCAACGTTTTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGTTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAT                                                                                      <  SRR19973070.964579/147‑1 (MQ=60)
                                                             GTGTTGCAGGATACCTTGCAAAAGCAACGTTTTTACTTGACGGCGAACAGCGCTCGTCTGTACCACCATTTAAACCTGTTGAAATAATCGGTAAACCGAAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAT                                                                                      <  SRR19973070.964925/150‑1 (MQ=60)
                                                             GTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAT                                                                                      <  SRR19973070.3317218/150‑1 (MQ=60)
                                                                   CAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCC                                                                                <  SRR19973070.1097537/150‑1 (MQ=60)
                                                                   CAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCC                                                                                >  SRR19973070.1494500/1‑150 (MQ=60)
                                                                    AGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCG                                                                               >  SRR19973070.1766792/1‑150 (MQ=60)
                                                                     GGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGC                                                                              >  SRR19973070.2013266/1‑150 (MQ=60)
                                                                      GATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCG                                                                             >  SRR19973070.2452829/1‑150 (MQ=60)
                                                                      GATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCG                                                                             >  SRR19973070.1356332/1‑150 (MQ=60)
                                                                       ATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGC                                                                            >  SRR19973070.707470/1‑150 (MQ=60)
                                                                            TTGCAAAAGCAACGTGATAACGGGACGGCGAACAGCCCTCGGCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAATTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATT                                                                       <  SRR19973070.1911875/150‑1 (MQ=60)
                                                                               CAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAG                                                                    <  SRR19973070.2140764/150‑1 (MQ=60)
                                                                                 AAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGC                                                                  >  SRR19973070.2572418/1‑150 (MQ=60)
                                                                                  AAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCT                                                                 >  SRR19973070.762121/1‑150 (MQ=60)
                                                                                  AAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCT                                                                 >  SRR19973070.762102/1‑150 (MQ=60)
                                                                                       ACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCC                                                            <  SRR19973070.2654018/150‑1 (MQ=60)
                                                                                       ACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCC                                                            <  SRR19973070.2654014/150‑1 (MQ=60)
                                                                                        CGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCG                                                           >  SRR19973070.1243902/1‑150 (MQ=60)
                                                                                        CGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCG                                                           >  SRR19973070.1243985/1‑150 (MQ=60)
                                                                                         GTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGC                                                          >  SRR19973070.1848595/1‑150 (MQ=60)
                                                                                          TGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCTGTTACAAGTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCT                                                         <  SRR19973070.2308986/150‑1 (MQ=60)
                                                                                          TGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCT                                                         <  SRR19973070.1916089/150‑1 (MQ=60)
                                                                                          TGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCT                                                         >  SRR19973070.170727/1‑150 (MQ=60)
                                                                                           GATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTG                                                        >  SRR19973070.2543048/1‑150 (MQ=60)
                                                                                           GATTACGGGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTG                                                        <  SRR19973070.636108/150‑1 (MQ=60)
                                                                                            ATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                       >  SRR19973070.517355/1‑150 (MQ=60)
                                                                                            ATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                       >  SRR19973070.1220865/1‑150 (MQ=60)
                                                                                             TTACGTGACGGCGAACTGCGCTGGTCTGGATCACCTGTTACAACTTTTGCAATAATCGGTAAACCGCAAGCGACTCACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGT                                                      <  SRR19973070.2409343/150‑1 (MQ=60)
                                                                                             TTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGT                                                      <  SRR19973070.818429/150‑1 (MQ=60)
                                                                                             TTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGT                                                      <  SRR19973070.1243394/150‑1 (MQ=60)
                                                                                             TTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGT                                                      <  SRR19973070.818871/150‑1 (MQ=60)
                                                                                               ACGTGAGGGCCAACAGCGCTCGTCTGGATCACCAGTAACAACTGTTGCAATAATCTTAAAAAAGCATGGGCTTGAGTTTAACCTAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGAGTATTCAGGCTAATCCGCTGGTGA                                                    <  SRR19973070.2805401/150‑1 (MQ=60)
                                                                                               ACGTGACGGCGAACAGCGCTCGTCGGGAGCACCTGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGA                                                    <  SRR19973070.2805400/150‑1 (MQ=60)
                                                                                                  TGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTCTTCAGGCTAATCCGCTGGTGAAGC                                                 >  SRR19973070.1744855/1‑150 (MQ=60)
                                                                                                       GCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAA                                            <  SRR19973070.1627608/150‑1 (MQ=60)
                                                                                                         GAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACT                                          >  SRR19973070.2597692/1‑150 (MQ=60)
                                                                                                         GAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACT                                          >  SRR19973070.771718/1‑150 (MQ=60)
                                                                                                         GAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACT                                          >  SRR19973070.771677/1‑150 (MQ=60)
                                                                                                         GAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACT                                          >  SRR19973070.320799/1‑150 (MQ=60)
                                                                                                            CAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGA                                       <  SRR19973070.415919/150‑1 (MQ=60)
                                                                                                            CAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGA                                       <  SRR19973070.415801/150‑1 (MQ=60)
                                                                                                             AGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAA                                      >  SRR19973070.896125/1‑150 (MQ=60)
                                                                                                               CGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAT                                    >  SRR19973070.2746160/1‑150 (MQ=60)
                                                                                                               CGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAT                                    <  SRR19973070.1745668/150‑1 (MQ=60)
                                                                                                                GCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATT                                   <  SRR19973070.3273261/150‑1 (MQ=60)
                                                                                                                GCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATT                                   <  SRR19973070.1501324/150‑1 (MQ=60)
                                                                                                                GCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGAAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATT                                   <  SRR19973070.1500883/150‑1 (MQ=60)
                                                                                                                   CGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAC                                >  SRR19973070.747893/1‑150 (MQ=60)
                                                                                                                   CGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAC                                >  SRR19973070.748435/1‑150 (MQ=60)
                                                                                                                      CTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAG                             <  SRR19973070.3242179/150‑1 (MQ=60)
                                                                                                                          AGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGT                         >  SRR19973070.2047929/1‑150 (MQ=60)
                                                                                                                          AGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCACCGGATGAATCAGCGCGTATTCAGGCTAATACGCTGGTGAAGCAGGAACTGGAAATTAAACAGCAGT                         >  SRR19973070.371180/1‑150 (MQ=60)
                                                                                                                            CACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTA                       <  SRR19973070.552380/150‑1 (MQ=60)
                                                                                                                              CCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAG                     <  SRR19973070.2193383/150‑1 (MQ=60)
                                                                                                                               CAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGT                    >  SRR19973070.637495/1‑150 (MQ=60)
                                                                                                                                 GTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCA                  <  SRR19973070.2326464/150‑1 (MQ=60)
                                                                                                                                     CAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGT              >  SRR19973070.661345/1‑150 (MQ=60)
                                                                                                                                     CAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGT              >  SRR19973070.1314744/1‑150 (MQ=60)
                                                                                                                                     CAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGT              >  SRR19973070.1207404/1‑150 (MQ=60)
                                                                                                                                      AACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTC             >  SRR19973070.2907915/1‑150 (MQ=60)
                                                                                                                                       ACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCT            <  SRR19973070.1340471/150‑1 (MQ=60)
                                                                                                                                         TGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGA          >  SRR19973070.834185/1‑150 (MQ=60)
                                                                                                                                             GCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAC      <  SRR19973070.1923186/150‑1 (MQ=60)
                                                                                                                                              CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC     >  SRR19973070.2842792/1‑150 (MQ=60)
                                                                                                                                              CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC     >  SRR19973070.2749990/1‑150 (MQ=60)
                                                                                                                                                AGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGC   >  SRR19973070.92636/1‑150 (MQ=60)
                                                                                                                                                AGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGC   >  SRR19973070.986146/1‑150 (MQ=60)
                                                                                                                                                 GAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCG  >  SRR19973070.409088/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCG  >  CP009273/482609‑482903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑