Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 482,757 G→T 100% A256S (GCG→TCG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273482,7570GT96.1% 528.9 / 12.3 181A256S (GCG→TCG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (93/81);  total (97/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 187 total aligned reads displayed.

GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG  >  CP009273/482612‑482901
                                                                                                                                                 |                                                                                                                                                
 cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCgg                                                                                                                                               >  1:3638018/1‑148 (MQ=255)
 cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCgg                                                                                                                                               >  2:1454793/1‑148 (MQ=255)
 cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCgg                                                                                                                                               >  1:174309/1‑148 (MQ=255)
    gCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTaa                                                                                                                                            >  1:1345424/1‑148 (MQ=255)
    gCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTaa                                                                                                                                            >  2:3389641/1‑148 (MQ=255)
      gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAAc                                                                                                                                          <  1:2297062/148‑1 (MQ=255)
      gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAAc                                                                                                                                          <  1:373005/148‑1 (MQ=255)
       ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACa                                                                                                                                         >  2:689503/1‑148 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                        <  2:3296336/148‑1 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                        >  2:222559/1‑148 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                        >  1:1811274/1‑148 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                        >  2:3782792/1‑148 (MQ=255)
          cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa                                                                                                                                      <  2:1317540/148‑1 (MQ=255)
          cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa                                                                                                                                      <  1:309279/148‑1 (MQ=255)
            gAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAg                                                                                                                                    >  2:1634242/1‑148 (MQ=255)
            gAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAg                                                                                                                                    >  1:453452/1‑148 (MQ=255)
                   gagaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGa                                                                                                                             <  1:1369828/148‑1 (MQ=255)
                     gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACt                                                                                                                           <  2:108988/148‑1 (MQ=255)
                      aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACtt                                                                                                                          >  2:3776331/1‑148 (MQ=255)
                      aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACtt                                                                                                                          <  2:2302252/148‑1 (MQ=255)
                       ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACttt                                                                                                                         >  2:2942063/1‑148 (MQ=255)
                       ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCCGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACttt                                                                                                                         >  1:1537030/1‑148 (MQ=255)
                        tGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTa                                                                                                                        <  1:2776810/148‑1 (MQ=255)
                          aCCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAAc                                                                                                                      >  2:735684/1‑148 (MQ=255)
                          aCCAGCAGCGTAAAAGCCTGGAAGGCAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATAACGTGACGGAGAACAGCGCTCGTCTGGAGCACCAGATACAACTGTTGCAAGAATCGGTCAACATCAAGCGCCTGACTTTAAc                                                                                                                      >  2:2651442/1‑148 (MQ=255)
                              gcagcGTAAAAGCCTGGAAGGGAACACCGTCTTGCATGATACCTTTCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaa                                                                                                                  <  1:2566104/148‑1 (MQ=255)
                              gcagcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaa                                                                                                                  <  1:1247691/148‑1 (MQ=255)
                               cagcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaa                                                                                                                 >  1:2947345/1‑148 (MQ=255)
                                  cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa                                                                                                              <  1:2329825/148‑1 (MQ=255)
                                  cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa                                                                                                              >  2:2740222/1‑148 (MQ=255)
                                  cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGAGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa                                                                                                              >  2:860757/1‑148 (MQ=255)
                                  cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGAAACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa                                                                                                              >  2:1374011/1‑148 (MQ=255)
                                   gTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAAc                                                                                                             <  2:1594607/148‑1 (MQ=255)
                                     aaaaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGAATTTAACCGAAAAAACgg                                                                                                           <  1:3368734/148‑1 (MQ=255)
                                            tGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGa                                                                                                    <  2:1507900/148‑1 (MQ=255)
                                            tGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCTCAGGa                                                                                                    <  2:3554573/148‑1 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCTGTTACAACTGTTGCAAGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                  <  1:3718031/148‑1 (MQ=255)
                                                aGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGcc                                                                                                >  1:2034785/1‑148 (MQ=255)
                                                 gggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCg                                                                                               >  2:3195998/1‑148 (MQ=255)
                                                  ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              <  2:672276/148‑1 (MQ=255)
                                                  ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              >  1:846819/1‑148 (MQ=255)
                                                     acacCTTCTTGCAGGATACCTGGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAATAATCGGTAAACAGCAAGCGCCTGACTTTAACCCAAAAAACGGCGCAGGAAGCCGtctc                                                                                           <  1:2419003/148‑1 (MQ=255)
                                                     acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCTAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtctc                                                                                           <  1:3351878/148‑1 (MQ=255)
                                                     acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAATAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtctc                                                                                           <  1:3356030/148‑1 (MQ=255)
                                                     acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATGACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtctc                                                                                           >  1:517829/1‑148 (MQ=255)
                                                      cacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTcc                                                                                          >  2:245674/1‑148 (MQ=255)
                                                        ccGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTcccc                                                                                        >  1:1005906/1‑148 (MQ=255)
                                                        ccGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTcccc                                                                                        >  1:1005900/1‑148 (MQ=255)
                                                           tCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGa                                                                                     >  1:3249308/1‑148 (MQ=255)
                                                            cTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAt                                                                                    >  2:3111073/1‑148 (MQ=255)
                                                            cTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAt                                                                                    >  2:3111262/1‑148 (MQ=255)
                                                              tGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGa                                                                                  <  2:2512015/148‑1 (MQ=255)
                                                                cAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAg                                                                                <  1:1524427/148‑1 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  2:1121196/1‑148 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  2:3028203/1‑148 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  1:2143790/1‑148 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTAAGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  1:2167468/1‑148 (MQ=255)
                                                                    aTACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgc                                                                            >  1:3380905/1‑148 (MQ=255)
                                                                     tACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcg                                                                           >  1:548722/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                          >  1:3052521/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                          >  1:375808/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                          >  2:2316352/1‑148 (MQ=255)
                                                                            cAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTc                                                                    <  2:3449582/148‑1 (MQ=255)
                                                                               aaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAgg                                                                 <  2:3638018/148‑1 (MQ=255)
                                                                                    aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt                                                            >  2:2701638/1‑148 (MQ=255)
                                                                                        gATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGc                                                        >  2:1190812/1‑148 (MQ=255)
                                                                                         aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt                                                       >  2:5096/1‑148 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                      <  2:1851448/148‑1 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                      <  2:1851423/148‑1 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                      >  2:1725209/1‑148 (MQ=255)
                                                                                           tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg                                                     >  2:3797202/1‑148 (MQ=255)
                                                                                           tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg                                                     >  1:3042036/1‑148 (MQ=255)
                                                                                                gACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAg                                                <  2:805095/148‑1 (MQ=255)
                                                                                                gACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAg                                                <  2:3114163/148‑1 (MQ=255)
                                                                                                 aCGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGACGc                                               >  2:1754724/1‑148 (MQ=255)
                                                                                                            cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                    <  1:222559/148‑1 (MQ=255)
                                                                                                            cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                    >  1:2956710/1‑148 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                              >  2:3759245/1‑148 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                              >  2:2796106/1‑148 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                              >  2:1666844/1‑148 (MQ=255)
                                                                                                                  tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc                              >  2:1666824/1‑148 (MQ=255)
                                                                                                                   cTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc                             >  2:12101/1‑148 (MQ=255)
                                                                                                                      gAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGTAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagc                          <  2:2228251/148‑1 (MQ=255)
                                                                                                                         cACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGt                       <  2:1596048/148‑1 (MQ=255)
                                                                                                                           ccAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa                     <  2:548722/148‑1 (MQ=255)
                                                                                                                            cAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTaa                    >  1:1842643/1‑148 (MQ=255)
                                                                                                                             aGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg                   <  2:1085704/148‑1 (MQ=255)
                                                                                                                                  cAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGc              >  1:3488571/1‑148 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt            >  1:2092868/1‑148 (MQ=255)
                                                                                                                                     cTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTc           <  1:112088/148‑1 (MQ=255)
                                                                                                                                      tGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCt          >  2:529884/1‑148 (MQ=255)
                                                                                                                                      tGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCt          >  2:529940/1‑148 (MQ=255)
                                                                                                                                        ttGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGa        >  2:3027826/1‑148 (MQ=255)
                                                                                                                                           cAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa     <  1:1888688/148‑1 (MQ=255)
                                                                                                                                           cAAGAAGCGGTTAACAGCACGGGCCTGACTTTAACCGAAAAAACGGCGCAGGTAGCCGTCTCCCCGGATGTTGCCGCGCGTAGTCAGGCTAATCCGCTGGTGAATCTGGATCTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa     <  2:2608571/148‑1 (MQ=255)
                                                                                                                                            aagaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAc    <  2:2707876/148‑1 (MQ=255)
                                                                                                                                              gaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg  >  1:146810/1‑148 (MQ=255)
                                                                                                                                              gaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg  >  2:443537/1‑148 (MQ=255)
                                                                                                                                              gaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg  >  1:3544722/1‑148 (MQ=255)
                                                                                                                                              gaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg  >  2:3568811/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG  >  CP009273/482612‑482901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC  >  CP009273/482609‑482905
                                                                                                                                                    |                                                                                                                                                    
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATC                                                                                                                                                     <  SRR19973069.747408/150‑1 (MQ=60)
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATC                                                                                                                                                     <  SRR19973069.2497988/150‑1 (MQ=60)
 CAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAATCAAATTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCG                                                                                                                                                    <  SRR19973069.3770100/150‑1 (MQ=60)
  AGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGG                                                                                                                                                   >  SRR19973069.3661866/1‑150 (MQ=60)
  AGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGG                                                                                                                                                   >  SRR19973069.3661829/1‑150 (MQ=60)
  AGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGG                                                                                                                                                   >  SRR19973069.175782/1‑150 (MQ=60)
   GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGT                                                                                                                                                  <  SRR19973069.1421824/150‑1 (MQ=60)
    CAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTA                                                                                                                                                 >  SRR19973069.1813735/1‑150 (MQ=60)
     AGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAA                                                                                                                                                >  SRR19973069.1354735/1‑150 (MQ=60)
     AGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAA                                                                                                                                                >  SRR19973069.2816593/1‑150 (MQ=60)
         GTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAG                                                                                                                                            >  SRR19973069.1824006/1‑150 (MQ=60)
         GTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAG                                                                                                                                            <  SRR19973069.2312667/150‑1 (MQ=60)
         GTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAG                                                                                                                                            <  SRR19973069.375726/150‑1 (MQ=60)
             CTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAG                                                                                                                                        <  SRR19973069.311353/150‑1 (MQ=60)
             CTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAG                                                                                                                                        >  SRR19973069.456769/1‑150 (MQ=60)
                  TGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCT                                                                                                                                   <  SRR19973069.1846198/150‑1 (MQ=60)
                      GAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACT                                                                                                                               <  SRR19973069.1379358/150‑1 (MQ=60)
                        GATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCCGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTT                                                                                                                             >  SRR19973069.1547572/1‑150 (MQ=60)
                        GATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCCGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTT                                                                                                                             >  SRR19973069.1379017/1‑150 (MQ=60)
                         ATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTA                                                                                                                            <  SRR19973069.1522911/150‑1 (MQ=60)
                         ATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTA                                                                                                                            >  SRR19973069.156018/1‑150 (MQ=60)
                           TGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAAC                                                                                                                          <  SRR19973069.2795307/150‑1 (MQ=60)
                                AGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAA                                                                                                                     >  SRR19973069.2966906/1‑150 (MQ=60)
                                AGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAA                                                                                                                     >  SRR19973069.3021940/1‑150 (MQ=60)
                                 GCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCATGATACCTTTCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAA                                                                                                                    <  SRR19973069.2583074/150‑1 (MQ=60)
                                 GCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAA                                                                                                                    <  SRR19973069.1256147/150‑1 (MQ=60)
                                 GCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAA                                                                                                                    <  SRR19973069.1256151/150‑1 (MQ=60)
                                     CGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACG                                                                                                                <  SRR19973069.2345675/150‑1 (MQ=60)
                                     CGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACG                                                                                                                <  SRR19973069.2345911/150‑1 (MQ=60)
                                       TAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGC                                                                                                              <  SRR19973069.3402441/150‑1 (MQ=60)
                                       TAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGC                                                                                                              <  SRR19973069.3648354/150‑1 (MQ=60)
                                        AAAAGCGTGGATGGGAACACCGTCTTGCAGGATACCTTGAAAAAGCAACGTGATTACGTGACGGCTAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCG                                                                                                             <  SRR19973069.1213516/150‑1 (MQ=60)
                                        AAAAGCCTGGAAGGTAACACCGTCTTGCAGTATACCTTGCAAAAGCAACGTGATTAGGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGAATTTAACCGAAAAAACGGCG                                                                                                             <  SRR19973069.3390761/150‑1 (MQ=60)
                                             CCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGA                                                                                                        >  SRR19973069.1123821/1‑150 (MQ=60)
                                               TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                      >  SRR19973069.3547335/1‑150 (MQ=60)
                                                 GAAGGTAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                    <  SRR19973069.2308750/150‑1 (MQ=60)
                                                 GAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCTGTTACAACTGTTGCAAGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                    <  SRR19973069.3742306/150‑1 (MQ=60)
                                                 GAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                    >  SRR19973069.2048913/1‑150 (MQ=60)
                                                 GAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCC                                                                                                    <  SRR19973069.3742308/150‑1 (MQ=60)
                                                   AGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGT                                                                                                  >  SRR19973069.852754/1‑150 (MQ=60)
                                                     GGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                >  SRR19973069.793247/1‑150 (MQ=60)
                                                      GAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTC                                                                                               >  SRR19973069.520360/1‑150 (MQ=60)
                                                      GAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATGACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTC                                                                                               >  SRR19973069.521508/1‑150 (MQ=60)
                                                        ACACCTTCTTGCAGGATACCTGGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAATAATCGGTAAACAGCAAGCGCCTGACTTTAACCCAAAAAACGGCGCAGGAAGCCGTCTCCC                                                                                             <  SRR19973069.2435342/150‑1 (MQ=60)
                                                        ACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCTAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCC                                                                                             <  SRR19973069.3373814/150‑1 (MQ=60)
                                                        ACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAATAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCC                                                                                             <  SRR19973069.3377989/150‑1 (MQ=60)
                                                        ACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCC                                                                                             <  SRR19973069.3373807/150‑1 (MQ=60)
                                                         CACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCC                                                                                            >  SRR19973069.1012825/1‑150 (MQ=60)
                                                         CACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCC                                                                                            >  SRR19973069.1012819/1‑150 (MQ=60)
                                                          ACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCG                                                                                           >  SRR19973069.306800/1‑150 (MQ=60)
                                                            CGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGA                                                                                         >  SRR19973069.3270579/1‑150 (MQ=60)
                                                                   CAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCC                                                                                  <  SRR19973069.1534926/150‑1 (MQ=60)
                                                                   CAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCC                                                                                  >  SRR19973069.1973581/1‑150 (MQ=60)
                                                                    agtatccgttgaAAAAGCAAGGTGTTTCCGTGACGGCCAACAGCGCTCGTCGGGAGCACCAGTTAAAAGTGTGGCACGAATCGGAAAACAGCACGCGCCTGACTTTAACCCAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCG                                                                                 <  SRR19973069.694324/138‑1 (MQ=60)
                                                                    AGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCG                                                                                 >  SRR19973069.2158505/1‑150 (MQ=60)
                                                                    AGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGAAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCG                                                                                 >  SRR19973069.2184293/1‑150 (MQ=60)
                                                                    AGGATACCTTGCAAAAGCAACGTGATTAAGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCG                                                                                 >  SRR19973069.2182280/1‑150 (MQ=60)
                                                                     GGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGC                                                                                >  SRR19973069.3402989/1‑150 (MQ=60)
                                                                      GATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCG                                                                               >  SRR19973069.552382/1‑150 (MQ=60)
                                                                      GATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCG                                                                               >  SRR19973069.552564/1‑150 (MQ=60)
                                                                       ATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGC                                                                              >  SRR19973069.378555/1‑150 (MQ=60)
                                                                       ATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGC                                                                              >  SRR19973069.3072719/1‑150 (MQ=60)
                                                                          CCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTA                                                                           >  SRR19973069.2596925/1‑150 (MQ=60)
                                                                                      AACGTGATTACGTGACGGCGAACAGCGCTCTTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATC                                                               <  SRR19973069.3478758/150‑1 (MQ=60)
                                                                                            ATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                         >  SRR19973069.3062194/1‑150 (MQ=60)
                                                                                                   GACGGCGAACAGCGCTCGTCGGGAGCACCAGTTACAACTTTTGCAAGAATCGGTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCA                                                  <  SRR19973069.1336302/150‑1 (MQ=60)
                                                                                                             AGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAA                                        >  SRR19973069.2976329/1‑150 (MQ=60)
                                                                                                             AGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAA                                        <  SRR19973069.3091307/150‑1 (MQ=60)
                                                                                                               CGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAT                                      <  SRR19973069.224276/150‑1 (MQ=60)
                                                                                                                   CGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAC                                  <  SRR19973069.2114835/150‑1 (MQ=60)
                                                                                                                     TCTGGAGCACCATTTAAAACTGTTGCAAGAATGGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCA                                <  SRR19973069.2316101/150‑1 (MQ=60)
                                                                                                                          AGCACCAGTTACAACTGTTGAAAGAATCGGTAAACCCCAAGCGCCTGAATTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGT                           <  SRR19973069.337644/150‑1 (MQ=60)
                                                                                                                           GCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGAAGTT                          <  SRR19973069.3149368/150‑1 (MQ=60)
                                                                                                                            CACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTA                         <  SRR19973069.247099/150‑1 (MQ=60)
                                                                                                                             ACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAA                        >  SRR19973069.1855525/1‑150 (MQ=60)
                                                                                                                                 GTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCA                    <  SRR19973069.3782346/150‑1 (MQ=60)
                                                                                                                                   TACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGC                  >  SRR19973069.3511076/1‑150 (MQ=60)
                                                                                                                                     CAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGT                >  SRR19973069.2107336/1‑150 (MQ=60)
                                                                                                                                        CTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTG             <  SRR19973069.113188/150‑1 (MQ=60)
                                                                                                                                              CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC       <  SRR19973069.1901592/150‑1 (MQ=60)
                                                                                                                                              CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC       <  SRR19973069.1901795/150‑1 (MQ=60)
                                                                                                                                               AAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG      >  SRR19973069.148121/1‑150 (MQ=60)
                                                                                                                                               AAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG      >  SRR19973069.3567743/1‑150 (MQ=60)
                                                                                                                                                  AATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGA   >  SRR19973069.2682758/1‑150 (MQ=60)
                                                                                                                                                  AATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGA   >  SRR19973069.2682785/1‑150 (MQ=60)
                                                                                                                                                   ATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC  >  SRR19973069.2110399/1‑150 (MQ=60)
                                                                                                                                                   ATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC  >  SRR19973069.947439/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC  >  CP009273/482609‑482905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑