Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 482,757 G→T 100% A256S (GCG→TCG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273482,7570GT98.0% 596.7 / 1.1 198A256S (GCG→TCG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (97/94);  total (99/99)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 204 total aligned reads displayed.

GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG  >  CP009273/482612‑482901
                                                                                                                                                 |                                                                                                                                                
gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCg                                                                                                                                                <  1:1177003/148‑1 (MQ=255)
 cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCgg                                                                                                                                               <  2:1156620/148‑1 (MQ=255)
   ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTa                                                                                                                                             <  2:1865790/148‑1 (MQ=255)
    gCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTaa                                                                                                                                            <  2:1716690/148‑1 (MQ=255)
       ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACa                                                                                                                                         <  2:479584/148‑1 (MQ=255)
       ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACa                                                                                                                                         >  1:2135631/1‑148 (MQ=255)
        tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg                                                                                                                                        <  2:2028068/148‑1 (MQ=255)
          cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa                                                                                                                                      <  1:1848613/148‑1 (MQ=255)
           tGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCaa                                                                                                                                     <  2:3200547/148‑1 (MQ=255)
             aaTGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgc                                                                                                                                   >  2:2538568/1‑148 (MQ=255)
                gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc                                                                                                                                >  2:2162894/1‑148 (MQ=255)
                  ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTg                                                                                                                              <  1:2100542/148‑1 (MQ=255)
                   gagaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGa                                                                                                                             >  1:2022648/1‑148 (MQ=255)
                    agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc                                                                                                                            >  1:3006312/1‑148 (MQ=255)
                    agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc                                                                                                                            >  1:2999293/1‑148 (MQ=255)
                        tGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTa                                                                                                                        <  2:1283892/148‑1 (MQ=255)
                         gACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTaa                                                                                                                       <  2:908326/148‑1 (MQ=255)
                          aCCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAAc                                                                                                                      >  2:1928923/1‑148 (MQ=255)
                           ccAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAAcc                                                                                                                     <  2:2957177/148‑1 (MQ=255)
                                    tAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACg                                                                                                            <  1:2860825/148‑1 (MQ=255)
                                        aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcgc                                                                                                        >  1:260103/1‑148 (MQ=255)
                                          ccTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAg                                                                                                      >  2:1261350/1‑148 (MQ=255)
                                          ccTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAg                                                                                                      >  2:350800/1‑148 (MQ=255)
                                              gAAGGGACCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                  >  2:579322/1‑148 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                  <  2:2345949/148‑1 (MQ=255)
                                              gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg                                                                                                  >  1:2829874/1‑148 (MQ=255)
                                               aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGc                                                                                                 <  2:3455409/148‑1 (MQ=255)
                                                aGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGcc                                                                                                >  2:3480415/1‑148 (MQ=255)
                                                 gggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCg                                                                                               >  1:2408055/1‑148 (MQ=255)
                                                 gggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCg                                                                                               >  2:516210/1‑148 (MQ=255)
                                                  ggAACACGGTGTTGCAGGATACCTTCCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCATTTACAACTGTTGAAATATTCGGTAAACCGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              <  1:648152/148‑1 (MQ=255)
                                                  ggAACACCTTCTTGCCGGATACCTTGCAAAAGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              <  1:2741878/148‑1 (MQ=255)
                                                  ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              >  2:2006088/1‑148 (MQ=255)
                                                  ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              <  1:2012917/148‑1 (MQ=255)
                                                  gcaaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACCGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt                                                                                              <  1:2472538/146‑1 (MQ=255)
                                                   taaCACCGTCTTGCAGTATACCTTGCAAAAGCAAGGTGATTACGTGACGGCGAACAGCGCTCGTCTGCAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtc                                                                                             <  1:399967/147‑1 (MQ=255)
                                                   gAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtc                                                                                             >  1:1104278/1‑148 (MQ=255)
                                                     acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtctc                                                                                           >  2:1383283/1‑148 (MQ=255)
                                                       acCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTccc                                                                                         <  2:2468598/148‑1 (MQ=255)
                                                          gTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCgg                                                                                      >  1:1507376/1‑148 (MQ=255)
                                                            cTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAt                                                                                    <  2:1252625/148‑1 (MQ=255)
                                                                 aGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGc                                                                               <  2:2157743/148‑1 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCTAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             <  2:1855765/148‑1 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  2:1428325/1‑148 (MQ=255)
                                                                   gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                             >  2:1925037/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                          >  2:2048522/1‑148 (MQ=255)
                                                                      aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgc                                                                          >  2:2878510/1‑148 (MQ=255)
                                                                           gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt                                                                     <  2:2012929/148‑1 (MQ=255)
                                                                               aaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACACAAAGCGCCTGACTTTAACCTAAAAAACGGCGCGGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAgg                                                                 <  1:178586/148‑1 (MQ=255)
                                                                               aaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAgg                                                                 <  2:133247/148‑1 (MQ=255)
                                                                               aaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAgg                                                                 >  1:1289874/1‑148 (MQ=255)
                                                                                aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc                                                                >  2:3007389/1‑148 (MQ=255)
                                                                                  cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa                                                              >  1:3405103/1‑148 (MQ=255)
                                                                                  cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa                                                              >  2:1965002/1‑148 (MQ=255)
                                                                                   atctttaTTACGTGACGGCTAACAGGTGTCGTATGGAGAACCTGTAAAATGTGTTTAAATAAACGTTAAACAGAACGCGCGTGAATTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTaa                                                             <  1:908531/142‑1 (MQ=255)
                                                                                       tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCg                                                         >  2:3044325/1‑148 (MQ=255)
                                                                                       tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCg                                                         >  2:3368131/1‑148 (MQ=255)
                                                                                       tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCg                                                         >  2:1601413/1‑148 (MQ=255)
                                                                                         aTTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGAAAGAATCGTAAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt                                                       <  1:69272/148‑1 (MQ=255)
                                                                                         aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt                                                       >  2:2715366/1‑148 (MQ=255)
                                                                                         aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt                                                       >  2:2350919/1‑148 (MQ=255)
                                                                                         aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt                                                       <  1:1096785/148‑1 (MQ=255)
                                                                                          ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                      >  2:2263905/1‑148 (MQ=255)
                                                                                             cGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTg                                                   >  1:2911163/1‑148 (MQ=255)
                                                                                              gTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGa                                                  <  2:758724/148‑1 (MQ=255)
                                                                                               tGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGaa                                                 <  1:2725920/148‑1 (MQ=255)
                                                                                                 aCGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGc                                               <  2:3379005/148‑1 (MQ=255)
                                                                                                   ggCGAACTGCGGTCGTCTTGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACCGCTAGCTCCTGACTTTATCCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAg                                             <  2:3109603/148‑1 (MQ=255)
                                                                                                   ggCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAg                                             >  2:2186526/1‑148 (MQ=255)
                                                                                                   ggCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAg                                             >  1:3434556/1‑148 (MQ=255)
                                                                                                   ggCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGCCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAg                                             >  2:2186420/1‑148 (MQ=255)
                                                                                                     cGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGa                                           <  2:2135429/148‑1 (MQ=255)
                                                                                                      gAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGaa                                          <  2:2408055/148‑1 (MQ=255)
                                                                                                            cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                    <  1:1077963/148‑1 (MQ=255)
                                                                                                                 gTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTaa                               <  1:2835445/148‑1 (MQ=255)
                                                                                                                 gTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTaa                               <  2:1787878/148‑1 (MQ=255)
                                                                                                                     cgAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcag                           <  2:3289895/147‑1 (MQ=255)
                                                                                                                     cgAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcag                           <  2:3289843/147‑1 (MQ=255)
                                                                                                                       aGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGAAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagca                         <  1:524880/148‑1 (MQ=255)
                                                                                                                        gCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagcag                        >  1:2241668/1‑148 (MQ=255)
                                                                                                                        gCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagcag                        >  1:409550/1‑148 (MQ=255)
                                                                                                                         cACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGt                       >  2:3237143/1‑148 (MQ=255)
                                                                                                                         cACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGt                       >  1:470695/1‑148 (MQ=255)
                                                                                                                           ccAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa                     >  2:1046893/1‑148 (MQ=255)
                                                                                                                            cAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTaa                    <  2:1698785/148‑1 (MQ=255)
                                                                                                                             aGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg                   <  1:687514/148‑1 (MQ=255)
                                                                                                                                 aCAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAg               <  2:1456908/148‑1 (MQ=255)
                                                                                                                                 aCAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAg               <  1:1709432/148‑1 (MQ=255)
                                                                                                                                   aaCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCg             <  1:3103589/148‑1 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt            <  1:451779/148‑1 (MQ=255)
                                                                                                                                    aCTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt            <  2:260103/148‑1 (MQ=255)
                                                                                                                                      tGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCt          >  2:720732/1‑148 (MQ=255)
                                                                                                                                       gTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTg         <  1:1173637/148‑1 (MQ=255)
                                                                                                                                       gTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTg         >  2:1836398/1‑148 (MQ=255)
                                                                                                                                        ttGCAAGACTCGGTAACCAGCAAGCGCCTGACTTGAACCGAACAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGa        <  2:3554691/148‑1 (MQ=255)
                                                                                                                                           cAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa     <  1:2893639/148‑1 (MQ=255)
                                                                                                                                           cAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa     <  2:2141327/148‑1 (MQ=255)
                                                                                                                                             agaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAcc   >  2:787685/1‑148 (MQ=255)
                                                                                                                                             agaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAcc   >  1:3314806/1‑148 (MQ=255)
                                                                                                                                              gaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg  >  1:776230/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG  >  CP009273/482612‑482901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACT  >  CP009273/482608‑482906
                                                                                                                                                     |                                                                                                                                                     
CCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAT                                                                                                                                                       <  SRR19973066.2768029/150‑1 (MQ=60)
CCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAT                                                                                                                                                       <  SRR19973066.2767393/150‑1 (MQ=60)
CCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAT                                                                                                                                                       >  SRR19973066.3401545/1‑150 (MQ=60)
    GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGT                                                                                                                                                   <  SRR19973066.1185292/150‑1 (MQ=60)
         CNTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACA                                                                                                                                              >  SRR19973066.2150241/1‑150 (MQ=60)
         CGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACA                                                                                                                                              >  SRR19973066.2150446/1‑150 (MQ=60)
              CTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAG                                                                                                                                         >  SRR19973066.632480/1‑150 (MQ=60)
              CTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAG                                                                                                                                         <  SRR19973066.1861846/150‑1 (MQ=60)
                   TGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCT                                                                                                                                    <  SRR19973066.3401476/150‑1 (MQ=60)
                     CGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGA                                                                                                                                  >  SRR19973066.2036794/1‑150 (MQ=60)
                      GGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAC                                                                                                                                 <  SRR19973066.2115002/150‑1 (MQ=60)
                      GGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAC                                                                                                                                 >  SRR19973066.3019202/1‑150 (MQ=60)
                      GGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAC                                                                                                                                 >  SRR19973066.3019133/1‑150 (MQ=60)
                      GGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAC                                                                                                                                 >  SRR19973066.3026307/1‑150 (MQ=60)
                         GATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTT                                                                                                                              <  SRR19973066.2753487/150‑1 (MQ=60)
                                CAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAA                                                                                                                       >  SRR19973066.510356/1‑150 (MQ=60)
                                        TAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGC                                                                                                               <  SRR19973066.2880158/150‑1 (MQ=60)
                                        TAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGC                                                                                                               <  SRR19973066.2880037/150‑1 (MQ=60)
                                         AAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCG                                                                                                              >  SRR19973066.2926977/1‑150 (MQ=60)
                                          AAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGC                                                                                                             >  SRR19973066.262060/1‑150 (MQ=60)
                                              CCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACGGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGA                                                                                                         >  SRR19973066.658075/1‑150 (MQ=60)
                                                TGTAAGGTAACACCTTATTGCAGTATACCTTGAAAAAGAAACGTTATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGAATTTAACCGAAAAAACGGCGCAGGAAG                                                                                                       <  SRR19973066.774110/150‑1 (MQ=60)
                                                TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                       >  SRR19973066.2848890/1‑150 (MQ=60)
                                                TGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAG                                                                                                       >  SRR19973066.1800624/1‑150 (MQ=60)
                                                   AAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCG                                                                                                    >  SRR19973066.2840984/1‑150 (MQ=60)
                                                   AAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCG                                                                                                    >  SRR19973066.2424352/1‑150 (MQ=60)
                                                     GGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTC                                                                                                  >  SRR19973066.1112283/1‑150 (MQ=60)
                                                      GTAACACCGTCTTGCAGGATACCTTGAAAAAGCAACGTTATTACGTGACGGCTAACAGCGCTCGTCTGGAGCACCAGTTAAAACTGTTGAAATAATCGGTAAACAGCAAGCGCCTGACTTTAACCTAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.2202730/150‑1 (MQ=60)
                                                      GGAACACGGTGTTGCAGGATACCTTCCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCATTTACAACTGTTGAAATATTCGGTAAACCGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.652849/150‑1 (MQ=60)
                                                      GGAACACCTTCTTGCCGGATACCTTGCAAAAGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.2760323/150‑1 (MQ=60)
                                                      GGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.2027017/150‑1 (MQ=60)
                                                      GGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGACTGGAGCACCAGTTACAACTGTTGCACGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.2027087/150‑1 (MQ=60)
                                                      GCAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACCGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCT                                                                                                 <  SRR19973066.2489396/150‑1 (MQ=60)
                                                       TAACACCGTCTTGCAGTATACCTTGCAAAAGCAAGGTGATTACGTGACGGCGAACAGCGCTCGTCTGCAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACACCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTC                                                                                                <  SRR19973066.403116/150‑1 (MQ=60)
                                                          CACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCC                                                                                             >  SRR19973066.2949545/1‑150 (MQ=60)
                                                           ACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAAGCGGTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCG                                                                                            <  SRR19973066.127048/150‑1 (MQ=60)
                                                            CCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGG                                                                                           >  SRR19973066.1517904/1‑150 (MQ=60)
                                                              GTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAT                                                                                         <  SRR19973066.1106727/150‑1 (MQ=60)
                                                              GTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGAT                                                                                         <  SRR19973066.1776022/150‑1 (MQ=60)
                                                                    CAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCC                                                                                   >  SRR19973066.1573493/1‑150 (MQ=60)
                                                                        ATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGC                                                                               >  SRR19973066.2417351/1‑150 (MQ=60)
                                                                           CCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTA                                                                            >  SRR19973066.2451148/1‑150 (MQ=60)
                                                                                 AAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGG                                                                      >  SRR19973066.1299207/1‑150 (MQ=60)
                                                                                   AAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGCAAGAATCGGTAAACACAAAGCGCCTGACTTTAACCTAAAAAACGGCGCGGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCT                                                                    <  SRR19973066.180156/150‑1 (MQ=60)
                                                                                    AGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTA                                                                   >  SRR19973066.3427838/1‑150 (MQ=60)
                                                                                    AGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTA                                                                   >  SRR19973066.1089831/1‑150 (MQ=60)
                                                                                    AGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTA                                                                   <  SRR19973066.1961255/150‑1 (MQ=60)
                                                                                       AACGTTTTTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCTGTTACAACTGTTGAAATAAGCGGTAAACACCAAGCGCCTGACTTTAACCTAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATC                                                                <  SRR19973066.2711875/150‑1 (MQ=60)
                                                                                        aggttATTACGTGACGGCTAACAGCGCTCGTCTGGACCACCATTTACAAGTGTTGCAATAATCGTTAAACAGCACGCGCCTGACTTTAACCTAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCC                                                               <  SRR19973066.302726/145‑1 (MQ=60)
                                                                                        ACGTGATTACGTGACGGCTAACAGCGCTCGTCGGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCC                                                               <  SRR19973066.3429983/150‑1 (MQ=60)
                                                                                        ACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCC                                                               <  SRR19973066.798395/150‑1 (MQ=60)
                                                                                            GATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTG                                                           >  SRR19973066.2871504/1‑150 (MQ=60)
                                                                                             ATTCCGTGCCGGCGAACCGCGCTCGTGTGCAGCACCATTTACAACTTTTGAAAGAATCGGTAATCAGCTAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                          <  SRR19973066.70081/150‑1 (MQ=60)
                                                                                             ATTCCGTGACGGCGAACAGCGCTCGTCTGGAGCACCATTTACAACTGTTGAAAGAATCGTAAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                          <  SRR19973066.70111/150‑1 (MQ=60)
                                                                                             ATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGG                                                          <  SRR19973066.1104758/150‑1 (MQ=60)
                                                                                               TACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTG                                                        >  SRR19973066.2930598/1‑150 (MQ=60)
                                                                                                   TGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGC                                                    <  SRR19973066.2744253/150‑1 (MQ=60)
                                                                                                   TGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGC                                                    >  SRR19973066.146106/1‑150 (MQ=60)
                                                                                                     ACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAG                                                  >  SRR19973066.3457534/1‑150 (MQ=60)
                                                                                                        GCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAA                                               <  SRR19973066.2525301/150‑1 (MQ=60)
                                                                                                          GAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACT                                             >  SRR19973066.443171/1‑150 (MQ=60)
                                                                                                                CGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAT                                       <  SRR19973066.1085846/150‑1 (MQ=60)
                                                                                                                     GTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACC                                  <  SRR19973066.2854500/150‑1 (MQ=60)
                                                                                                                     GTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACC                                  <  SRR19973066.2854454/150‑1 (MQ=60)
                                                                                                                          GAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAG                             >  SRR19973066.412765/1‑150 (MQ=60)
                                                                                                                          GAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAG                             >  SRR19973066.412584/1‑150 (MQ=60)
                                                                                                                          GAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAG                             >  SRR19973066.2257129/1‑150 (MQ=60)
                                                                                                                           AGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGT                            >  SRR19973066.474265/1‑150 (MQ=60)
                                                                                                                           AGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGT                            >  SRR19973066.474223/1‑150 (MQ=60)
                                                                                                                           AGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGAAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGT                            <  SRR19973066.528727/150‑1 (MQ=60)
                                                                                                                                 AGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTC                      <  SRR19973066.692567/150‑1 (MQ=60)
                                                                                                                                 AGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTC                      >  SRR19973066.3052998/1‑150 (MQ=60)
                                                                                                                                   TTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAG                    >  SRR19973066.1180713/1‑150 (MQ=60)
                                                                                                                                    TACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGC                   >  SRR19973066.2086245/1‑150 (MQ=60)
                                                                                                                                     ACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCG                  <  SRR19973066.1721834/150‑1 (MQ=60)
                                                                                                                                       AACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTC                <  SRR19973066.3124318/150‑1 (MQ=60)
                                                                                                                                        ACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCT               <  SRR19973066.455253/150‑1 (MQ=60)
                                                                                                                                        ACTGTTGCAACAATCGGAAAACACCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCT               <  SRR19973066.1886453/150‑1 (MQ=60)
                                                                                                                                           GTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGAT            <  SRR19973066.1181972/150‑1 (MQ=60)
                                                                                                                                           GTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGAT            <  SRR19973066.1181914/150‑1 (MQ=60)
                                                                                                                                           GTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGAT            <  SRR19973066.379818/150‑1 (MQ=60)
                                                                                                                                               CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC        <  SRR19973066.2912982/150‑1 (MQ=60)
                                                                                                                                               CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC        >  SRR19973066.3336811/1‑150 (MQ=60)
                                                                                                                                               CAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC        >  SRR19973066.3333507/1‑150 (MQ=60)
                                                                                                                                                AAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG       >  SRR19973066.781970/1‑150 (MQ=60)
                                                                                                                                                    ATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC   >  SRR19973066.1442001/1‑150 (MQ=60)
                                                                                                                                                    ATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGAC   >  SRR19973066.1446233/1‑150 (MQ=60)
                                                                                                                                                     TCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACT  <  SRR19973066.731085/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACT  >  CP009273/482608‑482906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑