Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,497,946 G→T 91.0% L7I (CTC→ATC)  fryA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,497,9460GT91.0% 382.9 / 32.6 145L7I (CTC→ATC) fryAputative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base T (67/65);  total (73/72)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 146 total aligned reads displayed.

GTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT  >  CP009273/2497801‑2498090
                                                                                                                                                 |                                                                                                                                                
gtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATaa                                                                                                                                                <  1:1219717/148‑1 (MQ=255)
 tgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATaaa                                                                                                                                               <  2:83952/148‑1 (MQ=255)
 tgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATaaa                                                                                                                                               <  2:512183/148‑1 (MQ=255)
   ccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAAtt                                                                                                                                             <  2:1243683/148‑1 (MQ=255)
     aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGa                                                                                                                                           >  2:602707/1‑148 (MQ=255)
      aTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGaa                                                                                                                                          <  2:2950271/148‑1 (MQ=255)
       tCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAAt                                                                                                                                         >  1:1867683/1‑148 (MQ=255)
        cAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATc                                                                                                                                        >  2:3003420/1‑148 (MQ=255)
         aGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCg                                                                                                                                       <  1:848332/148‑1 (MQ=255)
           cgcCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGtt                                                                                                                                     >  1:3313072/1‑148 (MQ=255)
                 cGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATa                                                                                                                               >  2:1444722/1‑148 (MQ=255)
                  gAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATaa                                                                                                                              <  1:2347787/148‑1 (MQ=255)
                   aGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAAc                                                                                                                             >  2:2896645/1‑148 (MQ=255)
                     cTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCg                                                                                                                           <  2:2080069/148‑1 (MQ=255)
                       tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCt                                                                                                                         >  1:2644722/1‑148 (MQ=255)
                       tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCt                                                                                                                         <  2:122165/148‑1 (MQ=255)
                        ttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTc                                                                                                                        <  2:2122721/148‑1 (MQ=255)
                          cGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCg                                                                                                                      <  1:1027339/148‑1 (MQ=255)
                             aTCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGt                                                                                                                   <  1:2919286/148‑1 (MQ=255)
                             aTCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGt                                                                                                                   <  1:2919280/148‑1 (MQ=255)
                                 gCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAAt                                                                                                               <  2:1537434/148‑1 (MQ=255)
                                 gCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAAt                                                                                                               >  2:14672/1‑148 (MQ=255)
                                 gCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAAt                                                                                                               <  2:860450/148‑1 (MQ=255)
                                  ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATc                                                                                                              <  1:576983/148‑1 (MQ=255)
                                    tttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                                                                                            >  1:2635214/1‑148 (MQ=255)
                                    tttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                                                                                            >  1:2635263/1‑148 (MQ=255)
                                     ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAgg                                                                                                           <  1:124154/148‑1 (MQ=255)
                                     ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAgg                                                                                                           >  1:1969032/1‑148 (MQ=255)
                                      tGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGga                                                                                                          >  1:2881621/1‑148 (MQ=255)
                                         gTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGat                                                                                                       <  2:446921/148‑1 (MQ=255)
                                           tCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatca                                                                                                     <  2:1348849/148‑1 (MQ=255)
                                            cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcat                                                                                                    <  1:2199318/148‑1 (MQ=255)
                                             tGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcatc                                                                                                   <  2:692412/148‑1 (MQ=255)
                                              ggCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCTTTAACATAACCGCTCCGGGTTAATAAGGAGATCATCt                                                                                                  >  2:686718/1‑148 (MQ=255)
                                               gCGATGGTTAATAAAAGTGATTGCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTg                                                                                                 <  2:2279420/148‑1 (MQ=255)
                                                cGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGa                                                                                                >  2:1218320/1‑148 (MQ=255)
                                                  aTGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGaaa                                                                                              >  2:856202/1‑148 (MQ=255)
                                                   tGGTTAATAACAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAAt                                                                                             <  1:652913/148‑1 (MQ=255)
                                                   tGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAAt                                                                                             <  1:652919/148‑1 (MQ=255)
                                                       taataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                         >  2:242278/1‑148 (MQ=255)
                                                       taataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                         >  2:242504/1‑148 (MQ=255)
                                                          taaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAg                                                                                      <  1:367163/148‑1 (MQ=255)
                                                          taaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAg                                                                                      >  2:476628/1‑148 (MQ=255)
                                                             aaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTg                                                                                   >  1:1520936/1‑148 (MQ=255)
                                                             aaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTg                                                                                   >  1:1156663/1‑148 (MQ=255)
                                                                tGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTATGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaac                                                                                >  2:2980210/1‑148 (MQ=255)
                                                                tGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaac                                                                                >  2:795969/1‑148 (MQ=255)
                                                                  aTTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaa                                                                              <  2:2690122/148‑1 (MQ=255)
                                                                          ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCa                                                                      >  1:1140926/1‑148 (MQ=255)
                                                                          ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCa                                                                      >  1:477753/1‑148 (MQ=255)
                                                                              cACTGGCAGCACTGTTCTTTAAGTTCCCACGCCGGACCAGAATTTAGACCGTAAGTCAAAGGACAGATAAATAGAATCGTTAACATAACCGCTCAGGGTTAATCAGGATATAATATGAAATCCTTCAGATGAACAAACGTCTAAagcg                                                                  >  2:1337330/1‑148 (MQ=255)
                                                                                cTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                                                <  2:1079646/148‑1 (MQ=255)
                                                                                cTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                                                <  2:873883/148‑1 (MQ=255)
                                                                                    cTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                                                            >  1:1213743/1‑148 (MQ=255)
                                                                                    cTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                                                            >  1:1216797/1‑148 (MQ=255)
                                                                                    cTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                                                            >  1:1734574/1‑148 (MQ=255)
                                                                                     tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           <  2:2689772/148‑1 (MQ=255)
                                                                                     tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           >  2:2955029/1‑148 (MQ=255)
                                                                                     tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           >  2:2031631/1‑148 (MQ=255)
                                                                                     tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           <  2:3313072/148‑1 (MQ=255)
                                                                                       cACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                         >  2:2961536/1‑148 (MQ=255)
                                                                                         cTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGa                                                       >  1:2682607/1‑148 (MQ=255)
                                                                                          tGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGaa                                                      >  1:2071462/1‑148 (MQ=255)
                                                                                          tGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGaa                                                      >  1:405722/1‑148 (MQ=255)
                                                                                           gTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAt                                                     >  1:1495652/1‑148 (MQ=255)
                                                                                           gTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAt                                                     >  1:3255963/1‑148 (MQ=255)
                                                                                             tCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                                                   >  2:3059623/1‑148 (MQ=255)
                                                                                             tCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                                                   >  2:2481247/1‑148 (MQ=255)
                                                                                              cTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAg                                                  <  2:1929745/148‑1 (MQ=255)
                                                                                                ttAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAggg                                                >  2:2474698/1‑148 (MQ=255)
                                                                                                   aGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAg                                             <  1:2896645/148‑1 (MQ=255)
                                                                                                        ccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaa                                        >  1:2641104/1‑148 (MQ=255)
                                                                                                        ccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaa                                        <  2:1519109/148‑1 (MQ=255)
                                                                                                         cACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaaa                                       <  1:919999/148‑1 (MQ=255)
                                                                                                             ccGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtg                                   <  2:721837/148‑1 (MQ=255)
                                                                                                             ccGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtg                                   <  1:1713641/148‑1 (MQ=255)
                                                                                                              gtgACGATCATGTAGACCGTTAGTCAAAGTACAGATTAATTGAATCGTTAACATAACCTCTCCGTGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAATAACCTTCTCATGCGTAAGTCTTTTAATAAGGGCAGATAAAAGTtgc                                  <  2:1573532/146‑1 (MQ=255)
                                                                                                              cGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgc                                  <  2:2644722/148‑1 (MQ=255)
                                                                                                                gACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATACGGGCAGAGAAAAGTtgctg                                >  2:3112449/1‑148 (MQ=255)
                                                                                                                gACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgctg                                >  1:3017038/1‑148 (MQ=255)
                                                                                                                gACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgctg                                >  1:3017040/1‑148 (MQ=255)
                                                                                                                gACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgctg                                >  1:1389173/1‑148 (MQ=255)
                                                                                                                 aCGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTCATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAATAGTtgctgc                               >  2:440894/1‑148 (MQ=255)
                                                                                                                     gCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCt                           <  2:1642185/148‑1 (MQ=255)
                                                                                                                        tGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCa                        >  2:1900231/1‑148 (MQ=255)
                                                                                                                        tGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCa                        >  2:1900246/1‑148 (MQ=255)
                                                                                                                        tGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCa                        <  2:152874/148‑1 (MQ=255)
                                                                                                                           aGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCaaaa                     <  1:1542532/148‑1 (MQ=255)
                                                                                                                           aGAACGTTAGGAAAAGGAAAGATAAATTGAATCGTTAAAATAACCTCTACGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAAGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCaaaa                     <  1:1542515/148‑1 (MQ=255)
                                                                                                                              ccGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGt                  >  2:1086234/1‑148 (MQ=255)
                                                                                                                              ccGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGt                  <  1:602707/148‑1 (MQ=255)
                                                                                                                                  tAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTcgcg              >  2:1829169/1‑148 (MQ=255)
                                                                                                                                     gCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggca           <  1:1809241/148‑1 (MQ=255)
                                                                                                                                     gCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggca           <  1:136295/148‑1 (MQ=255)
                                                                                                                                      cAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaa          >  2:2064878/1‑148 (MQ=255)
                                                                                                                                      cAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaa          >  2:709762/1‑148 (MQ=255)
                                                                                                                                       aaaGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaat         <  1:2493485/148‑1 (MQ=255)
                                                                                                                                       aaaGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaat         <  1:2493604/148‑1 (MQ=255)
                                                                                                                                         aGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg       >  1:1632749/1‑148 (MQ=255)
                                                                                                                                              agatAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggcaat  <  2:1713597/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT  >  CP009273/2497801‑2498090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGA  >  CP009273/2497797‑2498093
                                                                                                                                                     |                                                                                                                                                   
GCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAT                                                                                                                                                     <  SRR19973070.1014448/150‑1 (MQ=60)
    GTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAAT                                                                                                                                                 <  SRR19973070.1228206/150‑1 (MQ=60)
    GTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAAT                                                                                                                                                 <  SRR19973070.1228266/150‑1 (MQ=60)
        CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAA                                                                                                                                             >  SRR19973070.2932893/1‑150 (MQ=60)
         AATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAAT                                                                                                                                            >  SRR19973070.1880435/1‑150 (MQ=60)
           TCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCG                                                                                                                                          >  SRR19973070.2986943/1‑150 (MQ=60)
             AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTT                                                                                                                                        >  SRR19973070.3334256/1‑150 (MQ=60)
             AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTT                                                                                                                                        >  SRR19973070.342776/1‑150 (MQ=60)
             AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTT                                                                                                                                        <  SRR19973070.854134/150‑1 (MQ=60)
                      GAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACC                                                                                                                               <  SRR19973070.2363359/150‑1 (MQ=60)
                         CTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCT                                                                                                                            >  SRR19973070.2661947/1‑150 (MQ=60)
                              CGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGG                                                                                                                       <  SRR19973070.1034619/150‑1 (MQ=60)
                                 ATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTA                                                                                                                    <  SRR19973070.2938137/150‑1 (MQ=60)
                                 ATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTA                                                                                                                    <  SRR19973070.2938131/150‑1 (MQ=60)
                                 ATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTA                                                                                                                    <  SRR19973070.784668/150‑1 (MQ=60)
                                      CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                               >  SRR19973070.2652445/1‑150 (MQ=60)
                                      CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                               >  SRR19973070.2652396/1‑150 (MQ=60)
                                      CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                               <  SRR19973070.580933/150‑1 (MQ=60)
                                       CTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGG                                                                                                              >  SRR19973070.1982337/1‑150 (MQ=60)
                                       CTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGG                                                                                                              >  SRR19973070.1982151/1‑150 (MQ=60)
                                        TTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGA                                                                                                             >  SRR19973070.2900277/1‑150 (MQ=60)
                                         TTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAG                                                                                                            <  SRR19973070.125255/150‑1 (MQ=60)
                                                CTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCT                                                                                                     <  SRR19973070.2213820/150‑1 (MQ=60)
                                                       TGGTTAATAACAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCC                                                                                              <  SRR19973070.657568/150‑1 (MQ=60)
                                                       TGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCC                                                                                              <  SRR19973070.657574/150‑1 (MQ=60)
                                                       TGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCC                                                                                              <  SRR19973070.657215/150‑1 (MQ=60)
                                                        GGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCG                                                                                             <  SRR19973070.2550955/150‑1 (MQ=60)
                                                              TAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTT                                                                                       <  SRR19973070.369858/150‑1 (MQ=60)
                                                               AAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTG                                                                                      >  SRR19973070.1531610/1‑150 (MQ=60)
                                                               AAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTG                                                                                      >  SRR19973070.1164743/1‑150 (MQ=60)
                                                                            CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                         >  SRR19973070.481125/1‑150 (MQ=60)
                                                                            CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                         >  SRR19973070.1148843/1‑150 (MQ=60)
                                                                            CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                         >  SRR19973070.50346/1‑150 (MQ=60)
                                                                                    CTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAG                                                                 >  SRR19973070.1178404/1‑150 (MQ=60)
                                                                                      GGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAC                                                               >  SRR19973070.1222260/1‑150 (MQ=60)
                                                                                      GGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAC                                                               >  SRR19973070.1225325/1‑150 (MQ=60)
                                                                                      GGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAC                                                               >  SRR19973070.1746597/1‑150 (MQ=60)
                                                                                         TGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTT                                                            >  SRR19973070.319592/1‑150 (MQ=60)
                                                                                           CACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGA                                                          >  SRR19973070.2700009/1‑150 (MQ=60)
                                                                                            ACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAA                                                         >  SRR19973070.408699/1‑150 (MQ=60)
                                                                                            ACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAA                                                         >  SRR19973070.2085415/1‑150 (MQ=60)
                                                                                             CTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAT                                                        >  SRR19973070.1506118/1‑150 (MQ=60)
                                                                                             CTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAT                                                        >  SRR19973070.3276935/1‑150 (MQ=60)
                                                                                              TGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATA                                                       >  SRR19973070.1725439/1‑150 (MQ=60)
                                                                                                 TCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGG                                                    <  SRR19973070.2163891/150‑1 (MQ=60)
                                                                                                      AAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATAGTTAAAATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGA                                               <  SRR19973070.1764852/150‑1 (MQ=60)
                                                                                                       AGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGAT                                              <  SRR19973070.2915382/150‑1 (MQ=60)
                                                                                                          TCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAA                                           >  SRR19973070.2658970/1‑150 (MQ=60)
                                                                                                          TCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAA                                           >  SRR19973070.2658317/1‑150 (MQ=60)
                                                                                                             CACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGT                                        <  SRR19973070.926553/150‑1 (MQ=60)
                                                                                                               CGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTG                                      >  SRR19973070.1460135/1‑150 (MQ=60)
                                                                                                               CGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTG                                      <  SRR19973070.1305493/150‑1 (MQ=60)
                                                                                                               CGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTG                                      <  SRR19973070.2765770/150‑1 (MQ=60)
                                                                                                                 CCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCT                                    <  SRR19973070.1725484/150‑1 (MQ=60)
                                                                                                                  CGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTG                                   >  SRR19973070.3036280/1‑150 (MQ=60)
                                                                                                                  CGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTG                                   >  SRR19973070.1398558/1‑150 (MQ=60)
                                                                                                                  CGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTG                                   >  SRR19973070.3036282/1‑150 (MQ=60)
                                                                                                                       GAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCT                              <  SRR19973070.2553540/150‑1 (MQ=60)
                                                                                                                               AGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATG                      <  SRR19973070.1553373/150‑1 (MQ=60)
                                                                                                                               AGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATG                      <  SRR19973070.1553303/150‑1 (MQ=60)
                                                                                                                               AGAACGTTAGGAAAAGGAAAGATAAATTGAATCGTTAAAATAACCTCTACGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAAGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATG                      <  SRR19973070.1553356/150‑1 (MQ=60)
                                                                                                                                  CCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCG                   <  SRR19973070.606911/150‑1 (MQ=60)
                                                                                                                                         GCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAAT            <  SRR19973070.1821719/150‑1 (MQ=60)
                                                                                                                                         GCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAAT            <  SRR19973070.137483/150‑1 (MQ=60)
                                                                                                                                           AAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCG          >  SRR19973070.1644073/1‑150 (MQ=60)
                                                                                                                                           AAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCG          <  SRR19973070.2509779/150‑1 (MQ=60)
                                                                                                                                           AAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCG          <  SRR19973070.2470875/150‑1 (MQ=60)
                                                                                                                                           AAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCG          <  SRR19973070.2509660/150‑1 (MQ=60)
                                                                                                                                                  AGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCG   >  SRR19973070.596010/1‑150 (MQ=60)
                                                                                                                                                   GATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGA  >  SRR19973070.2037570/1‑150 (MQ=60)
                                                                                                                                                   GATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGA  >  SRR19973070.359998/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGA  >  CP009273/2497797‑2498093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑