Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,497,946	G→T	7.3%	L7I (CTC→ATC)	fryA ←	putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,497,946	0	G	T	7.3%	1058.1 / 35.8	279	L7I (CTC→ATC)	fryA	putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
Reads supporting (aligned to +/- strand): ref base G (134/124); new base T (12/9); total (146/133)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATG  >  CP009273/2497800‑2498095

ggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGaaa                                                                                                                                                    <  2:3527714/150‑1 (MQ=255)

  tgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAAtt                                                                                                                                                  <  1:3293832/150‑1 (MQ=255)

   gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTg                                                                                                                                                 <  1:4261410/150‑1 (MQ=255)

   gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTg                                                                                                                                                 >  1:2764890/1‑150 (MQ=255)

    ccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGa                                                                                                                                                <  1:286187/150‑1 (MQ=255)

     cAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGaa                                                                                                                                               >  2:2822070/1‑150 (MQ=255)

      aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAAt                                                                                                                                              >  2:4623339/1‑150 (MQ=255)

         cAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGt                                                                                                                                           >  2:1391909/1‑150 (MQ=255)

         cAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGt                                                                                                                                           >  2:1046350/1‑150 (MQ=255)

          aGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCTATGGACAGAGAAATTGAATCGtt                                                                                                                                          >  1:3164698/1‑150 (MQ=255)

          aGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGtt                                                                                                                                          >  1:847585/1‑150 (MQ=255)

             gcCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAAc                                                                                                                                       >  2:4584462/1‑150 (MQ=255)

              ccAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACa                                                                                                                                      <  1:1259719/150‑1 (MQ=255)

               cAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACAt                                                                                                                                     >  2:2567966/1‑150 (MQ=255)

                aGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATa                                                                                                                                    <  2:1855479/150‑1 (MQ=255)

                      cTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                                                                                              >  1:2566257/1‑150 (MQ=255)

                      cTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                                                                                              <  1:4140571/150‑1 (MQ=255)

                          tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCgg                                                                                                                          >  2:3899050/1‑150 (MQ=255)

                              aTCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTa                                                                                                                      >  1:4452146/1‑150 (MQ=255)

                               tCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTCAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTaa                                                                                                                     <  2:4212018/150‑1 (MQ=255)

                                   ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                                                                                                 >  2:2163284/1‑150 (MQ=255)

                                   ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                                                                                                 <  1:937116/150‑1 (MQ=255)

                                    cTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAgg                                                                                                                <  2:1765523/150‑1 (MQ=255)

                                     tttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGga                                                                                                               <  2:495743/150‑1 (MQ=255)

                                     tttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGga                                                                                                               >  2:741423/1‑150 (MQ=255)

                                        gCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGat                                                                                                            <  1:1711052/150‑1 (MQ=255)

                                            tCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcatc                                                                                                        >  1:1043204/1‑150 (MQ=255)

                                             cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCt                                                                                                       <  2:2358928/150‑1 (MQ=255)

                                                gCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGaa                                                                                                    >  1:4343832/1‑150 (MQ=255)

                                                  gATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAAt                                                                                                  <  1:2282583/150‑1 (MQ=255)

                                                    tGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATcc                                                                                                <  2:2625271/150‑1 (MQ=255)

                                                    tGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATcc                                                                                                >  2:597102/1‑150 (MQ=255)

                                                     ggTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCg                                                                                               <  2:1794535/150‑1 (MQ=255)

                                                      gTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                              >  2:1438557/1‑150 (MQ=255)

                                                      gTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                              >  2:2137049/1‑150 (MQ=255)

                                                      gTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAAACGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                              >  1:3624382/1‑150 (MQ=255)

                                                        taataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCa                                                                                            >  1:1227268/1‑150 (MQ=255)

                                                          ataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGt                                                                                          >  1:2355167/1‑150 (MQ=255)

                                                           taaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGtt                                                                                         >  1:3791462/1‑150 (MQ=255)

                                                            aaaaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTg                                                                                        <  1:2359979/150‑1 (MQ=255)

                                                               aGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTAGaac                                                                                     >  2:2234319/1‑150 (MQ=255)

                                                                 tGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaa                                                                                   >  2:4062035/1‑150 (MQ=255)

                                                                    tttCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCg                                                                                <  1:4604431/150‑1 (MQ=255)

                                                                      tCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtc                                                                              <  1:2728328/150‑1 (MQ=255)

                                                                       cGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtct                                                                             >  1:38224/1‑150 (MQ=255)

                                                                       cGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtct                                                                             >  1:3105756/1‑150 (MQ=255)

                                                                        gCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCATAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                                                            <  1:3190438/150‑1 (MQ=255)

                                                                        gCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                                                            >  2:4367796/1‑150 (MQ=255)

                                                                        gCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                                                            <  1:2358277/150‑1 (MQ=255)

                                                                           ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAcg                                                                         >  2:756479/1‑150 (MQ=255)

                                                                           ttGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCAcg                                                                         >  1:1812339/1‑150 (MQ=255)

                                                                               cACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                                                     >  2:2481846/1‑150 (MQ=255)

                                                                               cACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                                                     >  1:586182/1‑150 (MQ=255)

                                                                                aCTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTaa                                                                    <  2:3488843/150‑1 (MQ=255)

                                                                                aCTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTaa                                                                    >  1:2520870/1‑150 (MQ=255)

                                                                                 cTGGCTGCACTGTTCTTTAAGTTCAAACGCAGGACGAGCATGTAGAACGTTAGTCTAAGGACAGAGAAATTGAATTGTTAACATAACCGCTCCGGGTTAATCAGGAGATAATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAg                                                                   <  2:426983/150‑1 (MQ=255)

                                                                                   ggCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                                                                 <  2:3746255/150‑1 (MQ=255)

                                                                                      tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                              <  2:3625847/150‑1 (MQ=255)

                                                                                      tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                              >  2:3310318/1‑150 (MQ=255)

                                                                                      tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                              <  2:2959982/150‑1 (MQ=255)

                                                                                      tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                              <  1:3976628/150‑1 (MQ=255)

                                                                                       gCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTg                                                             >  1:2199161/1‑150 (MQ=255)

                                                                                        cACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGa                                                            <  2:252037/150‑1 (MQ=255)

                                                                                          cTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAt                                                          <  2:3822785/150‑1 (MQ=255)

                                                                                            gTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                                                        <  1:349900/150‑1 (MQ=255)

                                                                                              tCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAgg                                                      >  2:2857821/1‑150 (MQ=255)

                                                                                               cTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAggg                                                     <  2:1234606/150‑1 (MQ=255)

                                                                                                  tAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAg                                                  >  1:2435636/1‑150 (MQ=255)

                                                                                                    aGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGAt                                                >  1:1330535/1‑150 (MQ=255)

                                                                                                    aGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGAt                                                >  1:3757864/1‑150 (MQ=255)

                                                                                                    aGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGAt                                                <  1:499228/150‑1 (MQ=255)

                                                                                                      ttCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaa                                              >  1:1536697/1‑150 (MQ=255)

                                                                                                      ttCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaa                                              <  2:3099480/150‑1 (MQ=255)

                                                                                                       tCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaa                                             <  1:1425766/150‑1 (MQ=255)

                                                                                                        cccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaaa                                            >  2:4452177/1‑150 (MQ=255)

                                                                                                         ccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAg                                           >  1:595203/1‑150 (MQ=255)

                                                                                                          cACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGt                                          >  2:128094/1‑150 (MQ=255)

                                                                                                            cGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtg                                        <  1:1784022/150‑1 (MQ=255)

                                                                                                              ccGGACGAGCATGTAGACAGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgct                                      <  1:741600/150‑1 (MQ=255)

                                                                                                               cGGACGAGCATGTAGACAGTTAGGCAAAGGACAGAGAAATTTAATCGTTAACATAACCGCTCCTGGTTAATAAGGAGATCATCTGAAATCCGTCATTTGAACAACCGTCTCACGCGTAAGACGTGGAATAAGGGCAGATAAAAGTtgatg                                     >  1:207647/1‑150 (MQ=255)

                                                                                                                    gAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCt                                <  2:4534099/150‑1 (MQ=255)

                                                                                                                    gAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCt                                <  1:2810212/150‑1 (MQ=255)

                                                                                                                        aTGTAGACCGTTAGTCAAAGGACAGAGAAATTGAATCGTTAACATAACCTCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCTTCTAACGCGTAAGACGTTGAATAAGGGCATATAAAAGTTGCTGCATCTGCaa                            <  2:2795647/150‑1 (MQ=255)

                                                                                                                         tGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCaaa                           >  1:3259596/1‑150 (MQ=255)

                                                                                                                           tAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAAt                         <  2:1671674/150‑1 (MQ=255)

                                                                                                                           tAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAAt                         <  2:1671417/150‑1 (MQ=255)

                                                                                                                             gACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGt                       <  2:1481933/150‑1 (MQ=255)

                                                                                                                              aCCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTc                      >  1:2110837/1‑150 (MQ=255)

                                                                                                                               ccGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTcg                     >  1:4061950/1‑150 (MQ=255)

                                                                                                                                   tAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggc                 <  2:4043896/150‑1 (MQ=255)

                                                                                                                                    aGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggca                >  1:1987621/1‑150 (MQ=255)

                                                                                                                                        aaaGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg            >  2:2668336/1‑150 (MQ=255)

                                                                                                                                        aaaGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg            >  1:1673824/1‑150 (MQ=255)

                                                                                                                                        aaaGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg            >  1:3371091/1‑150 (MQ=255)

                                                                                                                                        aaaGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg            >  2:1113049/1‑150 (MQ=255)

                                                                                                                                             aCAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggcaat       >  1:994230/1‑150 (MQ=255)

                                                                                                                                              cAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCggcaatcggcaatc      >  2:2401538/1‑150 (MQ=255)

                                                                                                                                                  gaAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATg  <  1:4584619/150‑1 (MQ=255)

                                                                                                                                                  gaAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATg  <  2:2010398/150‑1 (MQ=255)

                                                                                                                                                  gaAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATg  <  1:398222/150‑1 (MQ=255)

GGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATG  >  CP009273/2497800‑2498095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A