Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,497,946 G→T 100% L7I (CTC→ATC)  fryA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,497,9460GT99.1% 662.5 / ‑3.3 205L7I (CTC→ATC) fryAputative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (100/103);  total (102/103)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

TGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT  >  CP009273/2497802‑2498090
                                                                                                                                                |                                                                                                                                                
tgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATaaa                                                                                                                                               <  2:4196/148‑1 (MQ=255)
    aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGa                                                                                                                                           <  2:3245870/148‑1 (MQ=255)
     aTCAGCTCCAGCGAGCGTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGaa                                                                                                                                          <  2:3353983/148‑1 (MQ=255)
     aTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGaa                                                                                                                                          <  2:384763/148‑1 (MQ=255)
     aTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGaa                                                                                                                                          >  1:1512078/1‑148 (MQ=255)
      tCAGCGCCAGCGACCAAAACCCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAAt                                                                                                                                         >  2:1270702/1‑148 (MQ=255)
        aGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCg                                                                                                                                       <  1:3349059/148‑1 (MQ=255)
          cgcCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGtt                                                                                                                                     <  1:183610/148‑1 (MQ=255)
                cGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATa                                                                                                                               >  2:3082596/1‑148 (MQ=255)
                 gAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATaa                                                                                                                              <  2:2290110/148‑1 (MQ=255)
                   gCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAAcc                                                                                                                            >  2:776274/1‑148 (MQ=255)
                      tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCt                                                                                                                         <  2:237635/148‑1 (MQ=255)
                      tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCt                                                                                                                         >  2:931848/1‑148 (MQ=255)
                      tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCt                                                                                                                         <  1:2996302/148‑1 (MQ=255)
                       ttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTc                                                                                                                        >  1:2715407/1‑148 (MQ=255)
                        tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTcc                                                                                                                       <  2:2094258/148‑1 (MQ=255)
                        tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTcc                                                                                                                       <  2:2652055/148‑1 (MQ=255)
                              cTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTa                                                                                                                 >  2:2782201/1‑148 (MQ=255)
                                 ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATc                                                                                                              <  1:1243202/148‑1 (MQ=255)
                                 ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATc                                                                                                              <  1:970932/148‑1 (MQ=255)
                                  cTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCa                                                                                                             >  1:978557/1‑148 (MQ=255)
                                  cTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCa                                                                                                             <  2:2217490/148‑1 (MQ=255)
                                   tttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                                                                                            >  1:78551/1‑148 (MQ=255)
                                    ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAgg                                                                                                           >  2:298893/1‑148 (MQ=255)
                                    ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAgg                                                                                                           >  2:300960/1‑148 (MQ=255)
                                           cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcat                                                                                                    <  1:1270702/148‑1 (MQ=255)
                                           cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcat                                                                                                    <  2:3318256/148‑1 (MQ=255)
                                            tGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGatcatc                                                                                                   <  2:1378688/148‑1 (MQ=255)
                                                 aTGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGgag                                                                                                         >  1:2150459/1‑137 (MQ=255)
                                                 aTGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGgag                                                                                                         <  2:2150459/137‑1 (MQ=255)
                                                 aTGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGaaa                                                                                              >  1:1005816/1‑148 (MQ=255)
                                                  tGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAAt                                                                                             <  1:1675659/148‑1 (MQ=255)
                                                   ggTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATc                                                                                            >  2:2017031/1‑148 (MQ=255)
                                                    gTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATcc                                                                                           >  2:551309/1‑148 (MQ=255)
                                                     ttAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCg                                                                                          >  1:2925070/1‑148 (MQ=255)
                                                      taataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                                                                                         <  1:418954/148‑1 (MQ=255)
                                                       aataaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTc                                                                                        >  2:1781939/1‑148 (MQ=255)
                                                         taaAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAg                                                                                      >  2:1243214/1‑148 (MQ=255)
                                                           aaaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGtt                                                                                    >  2:2033860/1‑148 (MQ=255)
                                                            aaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTg                                                                                   >  1:3228117/1‑148 (MQ=255)
                                                            aaGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTg                                                                                   >  2:2878440/1‑148 (MQ=255)
                                                                  tttCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaac                                                                             <  2:2257370/148‑1 (MQ=255)
                                                                      gCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtc                                                                         >  1:1280966/1‑148 (MQ=255)
                                                                       cTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtct                                                                        <  1:57511/148‑1 (MQ=255)
                                                                       cTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtct                                                                        <  1:829833/148‑1 (MQ=255)
                                                                        tttGCCACTGGCTGCACTGTTCTTTAAGGTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc                                                                       <  2:53743/148‑1 (MQ=255)
                                                                              aCTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGt                                                                 >  1:2606090/1‑148 (MQ=255)
                                                                              aCTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGt                                                                 >  1:907461/1‑148 (MQ=255)
                                                                               cTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGATCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa                                                                >  2:347349/1‑148 (MQ=255)
                                                                                 ggCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAg                                                              <  2:2278087/148‑1 (MQ=255)
                                                                                 ggCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAg                                                              <  2:1857850/148‑1 (MQ=255)
                                                                                  gCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGa                                                             >  1:722546/1‑148 (MQ=255)
                                                                                   cTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAc                                                            >  1:2462138/1‑148 (MQ=255)
                                                                                    tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           <  1:199521/148‑1 (MQ=255)
                                                                                    tGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACg                                                           <  1:708581/148‑1 (MQ=255)
                                                                                      cACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGtt                                                         <  2:2512682/148‑1 (MQ=255)
                                                                                         tGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGaa                                                      >  2:2792852/1‑148 (MQ=255)
                                                                                         tGTTCTTTAAGTTCCCACGCCGGACGAGAATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGaa                                                      <  1:3296592/148‑1 (MQ=255)
                                                                                          gTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAt                                                     >  2:1135177/1‑148 (MQ=255)
                                                                                           ttCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATa                                                    <  2:2662782/148‑1 (MQ=255)
                                                                                           ttCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATa                                                    <  2:1664650/148‑1 (MQ=255)
                                                                                            tCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATaa                                                   <  1:551309/148‑1 (MQ=255)
                                                                                               ttAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAggg                                                >  2:1867590/1‑148 (MQ=255)
                                                                                               ttAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAggg                                                >  1:1401481/1‑148 (MQ=255)
                                                                                                  aGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAg                                             <  2:1052311/148‑1 (MQ=255)
                                                                                                   gTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGa                                            >  2:255257/1‑148 (MQ=255)
                                                                                                      cccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaa                                         <  1:1781939/148‑1 (MQ=255)
                                                                                                       ccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaa                                        <  1:3405998/148‑1 (MQ=255)
                                                                                                       ccACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGAAGATaaa                                        >  2:1977366/1‑148 (MQ=255)
                                                                                                        cACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATaaaa                                       <  2:1675817/148‑1 (MQ=255)
                                                                                                         aCGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAg                                      <  2:1793901/148‑1 (MQ=255)
                                                                                                            ccGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtg                                   <  1:2016786/148‑1 (MQ=255)
                                                                                                             cGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgc                                  <  2:2942823/148‑1 (MQ=255)
                                                                                                             cGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgc                                  <  2:3142981/148‑1 (MQ=255)
                                                                                                             cGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgc                                  <  2:1395435/148‑1 (MQ=255)
                                                                                                               gACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgctg                                <  1:1116818/148‑1 (MQ=255)
                                                                                                                aCGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTtgctgc                               <  2:3290114/148‑1 (MQ=255)
                                                                                                                 cGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCa                              >  1:3032981/1‑148 (MQ=255)
                                                                                                                 cGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCa                              <  1:1364997/148‑1 (MQ=255)
                                                                                                                  gAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCAt                             <  2:579217/148‑1 (MQ=255)
                                                                                                                   aGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATc                            <  1:1063753/148‑1 (MQ=255)
                                                                                                                       tGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCa                        >  1:263799/1‑148 (MQ=255)
                                                                                                                        gTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCaa                       >  1:1326371/1‑148 (MQ=255)
                                                                                                                         tAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCaaa                      <  1:3068671/148‑1 (MQ=255)
                                                                                                                           gACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAAt                    <  2:1421742/148‑1 (MQ=255)
                                                                                                                                ttAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTcgc               <  2:2999494/148‑1 (MQ=255)
                                                                                                                                   ggCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggc            >  1:994139/1‑148 (MQ=255)
                                                                                                                                    gCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggca           >  2:1275248/1‑148 (MQ=255)
                                                                                                                                    gCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggca           <  2:464009/148‑1 (MQ=255)
                                                                                                                                       aaGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATACAAGTTGCTGCATCTGCAAAATGTCGcggcaatc        >  2:756656/1‑148 (MQ=255)
                                                                                                                                       aaGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatc        >  2:1565672/1‑148 (MQ=255)
                                                                                                                                        aGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcactcg       >  2:679219/1‑148 (MQ=255)
                                                                                                                                        aGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg       >  2:679519/1‑148 (MQ=255)
                                                                                                                                        aGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcg       >  1:1618130/1‑148 (MQ=255)
                                                                                                                                          gACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCAGTCAGTTGAACAACCGACTCACGCGTAAGACGTTGAATAAGGCCAGATAAAAGTTGCTGCATCTCCAAAATGTCGcggcaatcggc     >  2:2470626/1‑148 (MQ=255)
                                                                                                                                           aCAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggca    <  1:3082596/148‑1 (MQ=255)
                                                                                                                                           aCAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggca    <  1:2792852/148‑1 (MQ=255)
                                                                                                                                           aCAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggca    <  2:945493/148‑1 (MQ=255)
                                                                                                                                             agatAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggcaat  >  2:507564/1‑148 (MQ=255)
                                                                                                                                             agatAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGcggcaatcggcaat  >  2:3325188/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT  >  CP009273/2497802‑2498090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATG  >  CP009273/2497798‑2498095
                                                                                                                                                    |                                                                                                                                                     
CCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATA                                                                                                                                                      <  SRR19973065.2111503/150‑1 (MQ=60)
       CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAA                                                                                                                                               >  SRR19973065.1521938/1‑150 (MQ=60)
       CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAA                                                                                                                                               >  SRR19973065.1521935/1‑150 (MQ=60)
       CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAA                                                                                                                                               >  SRR19973065.2009876/1‑150 (MQ=60)
       CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAA                                                                                                                                               <  SRR19973065.714409/150‑1 (MQ=60)
          TCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCG                                                                                                                                            <  SRR19973065.2905278/150‑1 (MQ=60)
            AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTT                                                                                                                                          <  SRR19973065.3369522/150‑1 (MQ=60)
              CGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAA                                                                                                                                        <  SRR19973065.185062/150‑1 (MQ=60)
              CGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAA                                                                                                                                        <  SRR19973065.2174480/150‑1 (MQ=60)
               GCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAAC                                                                                                                                       <  SRR19973065.573368/150‑1 (MQ=60)
                    CGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATCAATTGAATCGTTAACATAAC                                                                                                                                  >  SRR19973065.951740/1‑150 (MQ=60)
                         TTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTC                                                                                                                             >  SRR19973065.2732300/1‑150 (MQ=60)
                          TTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCC                                                                                                                            <  SRR19973065.3014655/150‑1 (MQ=60)
                             CGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGG                                                                                                                         <  SRR19973065.81923/150‑1 (MQ=60)
                             CGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGG                                                                                                                         >  SRR19973065.4453/1‑150 (MQ=60)
                                ATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTNCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTA                                                                                                                      <  SRR19973065.1792518/150‑1 (MQ=60)
                                    GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCA                                                                                                                  >  SRR19973065.1994739/1‑150 (MQ=60)
                                    GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCA                                                                                                                  >  SRR19973065.985038/1‑150 (MQ=60)
                                    GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCA                                                                                                                  >  SRR19973065.985051/1‑150 (MQ=60)
                                    GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCA                                                                                                                  >  SRR19973065.987777/1‑150 (MQ=60)
                                     CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                                 >  SRR19973065.79345/1‑150 (MQ=60)
                                     CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                                 <  SRR19973065.977377/150‑1 (MQ=60)
                                     CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                                 >  SRR19973065.2679260/1‑150 (MQ=60)
                                     CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAG                                                                                                                 <  SRR19973065.1251376/150‑1 (MQ=60)
                                            GTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCA                                                                                                          <  SRR19973065.1640652/150‑1 (MQ=60)
                                              TCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATC                                                                                                        <  SRR19973065.781290/150‑1 (MQ=60)
                                               CTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCT                                                                                                       <  SRR19973065.1279084/150‑1 (MQ=60)
                                               CTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCT                                                                                                       <  SRR19973065.1279338/150‑1 (MQ=60)
                                                   CGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATagatcggaa                                                                                                   >  SRR19973065.2164288/1‑141 (MQ=60)
                                                   CGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAA                                                                                                   >  SRR19973065.1012494/1‑150 (MQ=60)
                                                    GATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAAT                                                                                                  <  SRR19973065.2951432/150‑1 (MQ=60)
                                                      TGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCC                                                                                                <  SRR19973065.1686871/150‑1 (MQ=60)
                                                       GGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCG                                                                                               >  SRR19973065.1371735/1‑150 (MQ=60)
                                                       GGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCG                                                                                               >  SRR19973065.2942841/1‑150 (MQ=60)
                                                          TAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCA                                                                                            <  SRR19973065.421881/150‑1 (MQ=60)
                                                              AAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTG                                                                                        >  SRR19973065.3247494/1‑150 (MQ=60)
                                                              AAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTG                                                                                        >  SRR19973065.3247499/1‑150 (MQ=60)
                                                                  GTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCGGTTGAACA                                                                                    >  SRR19973065.1518814/1‑150 (MQ=60)
                                                                        TCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTC                                                                              >  SRR19973065.1289422/1‑150 (MQ=60)
                                                                          GCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTC                                                                            >  SRR19973065.3047867/1‑150 (MQ=60)
                                                                          GCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTC                                                                            >  SRR19973065.1290908/1‑150 (MQ=60)
                                                                          GCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTC                                                                            >  SRR19973065.3374865/1‑150 (MQ=60)
                                                                           CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                           <  SRR19973065.58127/150‑1 (MQ=60)
                                                                           CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                           <  SRR19973065.835255/150‑1 (MQ=60)
                                                                           CTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCA                                                                           >  SRR19973065.873222/1‑150 (MQ=60)
                                                                                CCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGT                                                                      >  SRR19973065.2622261/1‑150 (MQ=60)
                                                                                CCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGT                                                                      >  SRR19973065.2622272/1‑150 (MQ=60)
                                                                                CCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGT                                                                      >  SRR19973065.913343/1‑150 (MQ=60)
                                                                                    TGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGA                                                                  >  SRR19973065.727383/1‑150 (MQ=60)
                                                                                     GGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGAC                                                                 >  SRR19973065.2477659/1‑150 (MQ=60)
                                                                                        TGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTT                                                              <  SRR19973065.3177011/150‑1 (MQ=60)
                                                                                        TGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTT                                                              <  SRR19973065.201066/150‑1 (MQ=60)
                                                                                        TGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTT                                                              <  SRR19973065.713210/150‑1 (MQ=60)
                                                                                        TGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTT                                                              <  SRR19973065.713312/150‑1 (MQ=60)
                                                                                         GCACTGTTCTTTAAGGTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTG                                                             <  SRR19973065.1842674/150‑1 (MQ=60)
                                                                                            CTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAAT                                                          <  SRR19973065.404253/150‑1 (MQ=60)
                                                                                             TGTTCTTTAAGTTCCCACGCCGGACGAGAATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATA                                                         <  SRR19973065.3316670/150‑1 (MQ=60)
                                                                                                TCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGG                                                      <  SRR19973065.554851/150‑1 (MQ=60)
                                                                                                 CTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGG                                                     >  SRR19973065.3337426/1‑150 (MQ=60)
                                                                                                 CTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGG                                                     >  SRR19973065.1410671/1‑150 (MQ=60)
                                                                                                          CCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTNAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAA                                            <  SRR19973065.1794007/150‑1 (MQ=60)
                                                                                                          CCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTGAACATAACCGCTCCGGGTNAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAA                                            <  SRR19973065.1793585/150‑1 (MQ=60)
                                                                                                           CCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAG                                           <  SRR19973065.3426807/150‑1 (MQ=60)
                                                                                                             ACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTT                                         <  SRR19973065.849673/150‑1 (MQ=60)
                                                                                                                CCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCT                                      <  SRR19973065.2029777/150‑1 (MQ=60)
                                                                                                                   GACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCA                                   >  SRR19973065.3052647/1‑150 (MQ=60)
                                                                                                                   GACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCA                                   >  SRR19973065.3051624/1‑150 (MQ=60)
                                                                                                                   GACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCA                                   >  SRR19973065.3051558/1‑150 (MQ=60)
                                                                                                                   GACGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCA                                   <  SRR19973065.1124110/150‑1 (MQ=60)
                                                                                                                     CGAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATC                                 <  SRR19973065.1374023/150‑1 (MQ=60)
                                                                                                                      GAGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCT                                <  SRR19973065.3090826/150‑1 (MQ=60)
                                                                                                                       AGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTG                               <  SRR19973065.1070804/150‑1 (MQ=60)
                                                                                                                       AGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTG                               <  SRR19973065.1070939/150‑1 (MQ=60)
                                                                                                                       AGCATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTG                               >  SRR19973065.2063098/1‑150 (MQ=60)
                                                                                                                         CATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCA                             >  SRR19973065.265609/1‑150 (MQ=60)
                                                                                                                         CATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCA                             >  SRR19973065.1266379/1‑150 (MQ=60)
                                                                                                                          ATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAA                            >  SRR19973065.1044746/1‑150 (MQ=60)
                                                                                                                          ATGTAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAA                            >  SRR19973065.1335138/1‑150 (MQ=60)
                                                                                                                             TAGACCGTTAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAAT                         <  SRR19973065.3087430/150‑1 (MQ=60)
                                                                                                                                     TAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGC                 >  SRR19973065.2575846/1‑150 (MQ=60)
                                                                                                                                     TAGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGC                 >  SRR19973065.1000749/1‑150 (MQ=60)
                                                                                                                                      AGGCAAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCA                >  SRR19973065.1212805/1‑150 (MQ=60)
                                                                                                                                          AAAGGACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCG            >  SRR19973065.1628985/1‑150 (MQ=60)
                                                                                                                                               ACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT       <  SRR19973065.3101421/150‑1 (MQ=60)
                                                                                                                                               ACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT       <  SRR19973065.2810163/150‑1 (MQ=60)
                                                                                                                                               ACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT       <  SRR19973065.2809334/150‑1 (MQ=60)
                                                                                                                                               ACAGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAAT       >  SRR19973065.1389691/1‑150 (MQ=60)
                                                                                                                                                 AGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCG     >  SRR19973065.2543267/1‑150 (MQ=60)
                                                                                                                                                 AGATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCG     >  SRR19973065.1726544/1‑150 (MQ=60)
                                                                                                                                                  GATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGA    >  SRR19973065.2124176/1‑150 (MQ=60)
                                                                                                                                                   ATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGAT   <  SRR19973065.1576205/150‑1 (MQ=60)
                                                                                                                                                   ATAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGAT   <  SRR19973065.2914607/150‑1 (MQ=60)
                                                                                                                                                   ATAAATTGAATCGTTAACATAACCGATCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGAT   <  SRR19973065.349779/150‑1 (MQ=60)
                                                                                                                                                   ATAAATTGAATCGTTAACATAACCGATCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGAT   <  SRR19973065.2131371/150‑1 (MQ=60)
                                                                                                                                                    TAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATG  >  SRR19973065.288224/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATG  >  CP009273/2497798‑2498095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑