Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,880 T→A 84.0% N202Y (AAC→TAC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,8800TA84.0% 326.1 / 51.5 130N202Y (AAC→TAC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (8/13);  new base A (72/37);  total (80/50)
Fisher's exact test for biased strand distribution p-value = 2.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 134 total aligned reads displayed.

TTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699737‑2700024
                                                                                                                                               |                                                                                                                                                
ttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCg                                                                                                                                              >  1:1815823/1‑148 (MQ=255)
  cagtGCGGTCACCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGaa                                                                                                                                            <  2:1463730/148‑1 (MQ=255)
    gtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAAcc                                                                                                                                          >  2:27310/1‑148 (MQ=255)
     tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         >  2:610693/1‑148 (MQ=255)
       cGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATg                                                                                                                                       <  1:2436007/148‑1 (MQ=255)
          tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                    >  1:3245679/1‑148 (MQ=255)
             aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 >  2:5838/1‑148 (MQ=255)
              ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACCCCCAGGTAGTAGTAGAAACAAGAGTGGAt                                                                                                                                >  1:2815205/1‑148 (MQ=255)
              ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCgtt                                                                                                                                >  2:3111191/1‑146 (MQ=255)
              ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:2131344/1‑148 (MQ=255)
              ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:2131449/1‑148 (MQ=255)
               cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATa                                                                                                                               >  2:3288141/1‑148 (MQ=255)
                tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              >  2:1875026/1‑148 (MQ=255)
                tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              >  2:1135574/1‑148 (MQ=255)
                tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              >  2:1261866/1‑148 (MQ=255)
                 gTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAt                                                                                                                             <  2:2883561/148‑1 (MQ=255)
                   ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTa                                                                                                                           >  1:2348896/1‑148 (MQ=255)
                    tCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAg                                                                                                                          <  1:539201/148‑1 (MQ=255)
                     cGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGt                                                                                                                         <  1:3271571/148‑1 (MQ=255)
                     cGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGt                                                                                                                         <  1:3288141/148‑1 (MQ=255)
                      ggCGTGAAGAGGCCCAGCCGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATCCGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTg                                                                                                                        <  1:1235729/148‑1 (MQ=255)
                        cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      >  2:2544001/1‑148 (MQ=255)
                        cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      >  2:2544287/1‑148 (MQ=255)
                          tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    >  2:3225193/1‑148 (MQ=255)
                           gAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGc                                                                                                                   >  2:3106765/1‑148 (MQ=255)
                            aaGAGGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCa                                                                                                                  <  2:799629/148‑1 (MQ=255)
                              gATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAAc                                                                                                                >  2:2923311/1‑148 (MQ=255)
                               aTGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACg                                                                                                               >  2:398723/1‑148 (MQ=255)
                                  cccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGTTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTGGATAATTATTGCCTGCAACGGGc                                                                                                            >  1:2388172/1‑148 (MQ=255)
                                   ccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                                                                           <  1:1955127/148‑1 (MQ=255)
                                   ccAGACGGTCGGCGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                                                                           <  1:2629255/148‑1 (MQ=255)
                                      gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTATTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTc                                                                                                        >  1:2273230/1‑148 (MQ=255)
                                          gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                                                                    >  2:1570881/1‑148 (MQ=255)
                                              gCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGc                                                                                                <  1:452964/148‑1 (MQ=255)
                                              gCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGc                                                                                                <  1:3268372/148‑1 (MQ=255)
                                                  tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            <  2:2809582/148‑1 (MQ=255)
                                                  tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            <  2:3132953/148‑1 (MQ=255)
                                                    taGGTCTGCCCGTTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGTATAAGTTAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcg                                                                                          <  1:23330/148‑1 (MQ=255)
                                                       gTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcggcg                                                                                       >  2:3261242/1‑148 (MQ=255)
                                                            cccGGTACTCATGACTTTCACTTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGa                                                                                  <  2:3093443/148‑1 (MQ=255)
                                                            cccGGTACTCATGACTTACACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGa                                                                                  <  1:539146/148‑1 (MQ=255)
                                                            cccGGTACACATGACTTCCACTGGGTCGCCCTTCCGCAGGGGGCGGTTTTTAAGCCGGATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGAGCGCCTTCCGGCGGCGGa                                                                                  <  1:1782242/148‑1 (MQ=255)
                                                                  aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            <  2:3062598/148‑1 (MQ=255)
                                                                  aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGCATGTcg                                                                            >  2:2865025/1‑148 (MQ=255)
                                                                  aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            >  2:676040/1‑148 (MQ=255)
                                                                   cTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcgc                                                                           >  2:198221/1‑148 (MQ=255)
                                                                    tCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCATCCGGCGGCGGAATGTcgcg                                                                          >  2:2772887/1‑148 (MQ=255)
                                                                     cATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCgcgc                                                                         >  2:1017356/1‑148 (MQ=255)
                                                                      aTGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGTTAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTTCCTGCAAAGGGCCTTCCGGATCGCCTTACCGCGGCGGAATGTCGCGCa                                                                        >  2:1414622/1‑148 (MQ=255)
                                                                      aTGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCa                                                                        >  2:789268/1‑148 (MQ=255)
                                                                      aTGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCa                                                                        >  1:2631826/1‑148 (MQ=255)
                                                                       tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAc                                                                       >  2:410374/1‑148 (MQ=255)
                                                                           tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGa                                                                   <  1:1276882/148‑1 (MQ=255)
                                                                           tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCGTCCGGCGGCGGAATGTCGCGCACCAGa                                                                   >  1:1836078/1‑148 (MQ=255)
                                                                             tCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAAATAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACg                                                                 >  1:1071127/1‑148 (MQ=255)
                                                                                cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                              >  2:2434623/1‑148 (MQ=255)
                                                                                 tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCg                                                             <  2:551384/148‑1 (MQ=255)
                                                                                      cGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCACACGTTCGAGAAc                                                        >  1:2366731/1‑148 (MQ=255)
                                                                                      cGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGTTAATTAGTGCCTGCAACGGGGCGTCCGGATCGCCTTCCGGCGGCGGGATGTCGCGCCCCAGACGGTCGAGAAc                                                        >  1:991655/1‑148 (MQ=255)
                                                                                      cGCCCTTACGCAGGGTGCCGTGTTTAATAAGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGAGAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCTTCCGGCGGCGGAAATTCGCGCACCACACGTTCGAGAAc                                                        >  1:991653/1‑148 (MQ=255)
                                                                                       gCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGCACg                                                       >  2:2864961/1‑148 (MQ=255)
                                                                                       gCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACg                                                       >  2:2864972/1‑148 (MQ=255)
                                                                                        cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGCTAGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt                                                      >  1:2047254/1‑148 (MQ=255)
                                                                                        cccTTACGCAGGGTGCCGTGTTTAAAACGGATAAGTGAAACAACGCCCCGGTAGTTGTCGAACCAGGTGTCGATAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCTTACGGCGGCGGCAATTCGCGCACCCCACGGTCGAGGACgg                                                      >  1:1508606/1‑147 (MQ=255)
                                                                                         ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc                                                     <  2:2060594/148‑1 (MQ=255)
                                                                                         ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc                                                     >  2:74846/1‑148 (MQ=255)
                                                                                          cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCAGACGTTCGAGCACGTcc                                                    >  2:2296755/1‑148 (MQ=255)
                                                                                          cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc                                                    >  2:2296775/1‑148 (MQ=255)
                                                                                          cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc                                                    >  2:2296278/1‑148 (MQ=255)
                                                                                          cTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAATTTCGCGCACCACACGTTCGAGAACCTcc                                                    >  1:3148140/1‑148 (MQ=255)
                                                                                                       ccGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTATTCGAAACATGAGTCGATAATTAGTGCCTGCAAACGGCCTTACCGATCGCCTTCCCGCGGCGGAATGTCGCGCACCAGAACTTACAGAACCTCCAGCACACCAAccc                                       >  1:425734/1‑146 (MQ=255)
                                                                                                       ccGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACCTCCTGCACACCAAccc                                       >  1:2627578/1‑146 (MQ=255)
                                                                                                          tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg                                    <  2:3042160/148‑1 (MQ=255)
                                                                                                          tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg                                    >  2:210420/1‑148 (MQ=255)
                                                                                                          tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg                                    >  1:2569956/1‑148 (MQ=255)
                                                                                                           ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                   <  1:2923311/148‑1 (MQ=255)
                                                                                                            tttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  2:1879899/148‑1 (MQ=255)
                                                                                                            tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  2:302081/148‑1 (MQ=255)
                                                                                                            tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  1:2183332/148‑1 (MQ=255)
                                                                                                            tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  >  2:188973/1‑148 (MQ=255)
                                                                                                             ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                 >  1:2485692/1‑148 (MQ=255)
                                                                                                             ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                 >  1:2484302/1‑148 (MQ=255)
                                                                                                                  aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTACCCCCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                            >  2:2819159/1‑148 (MQ=255)
                                                                                                                      aTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAc                        <  2:1585214/148‑1 (MQ=255)
                                                                                                                       tAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACa                       >  2:1892816/1‑148 (MQ=255)
                                                                                                                          gTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcg                    <  2:2363049/148‑1 (MQ=255)
                                                                                                                             aaaCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc                 <  1:1986348/148‑1 (MQ=255)
                                                                                                                             aaaCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc                 <  1:1135574/148‑1 (MQ=255)
                                                                                                                                caacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcgc              >  1:1073160/1‑148 (MQ=255)
                                                                                                                                  acGCCCAGGTAGTTGTCGAACCATGAGTCGATAATGAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCCACGCCGGTTTTCGCTGAACAGCGCACCGCGt            <  2:1412769/148‑1 (MQ=255)
                                                                                                                                  acGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGt            <  1:924100/148‑1 (MQ=255)
                                                                                                                                  acGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGt            >  1:1838726/1‑148 (MQ=255)
                                                                                                                                   cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc           >  1:945274/1‑148 (MQ=255)
                                                                                                                                   cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGACCAGCGCACCGCGTc           >  2:302362/1‑148 (MQ=255)
                                                                                                                                     cccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCgg         <  2:475909/148‑1 (MQ=255)
                                                                                                                                       cAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTg       >  1:2975053/1‑148 (MQ=255)
                                                                                                                                          gtagtaGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCg    >  1:169385/1‑148 (MQ=255)
                                                                                                                                          gtagtaGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCg    >  1:169079/1‑148 (MQ=255)
                                                                                                                                           tagtagTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGt   <  1:610693/148‑1 (MQ=255)
                                                                                                                                            agtagtCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTc  >  2:2429007/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699737‑2700024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  >  CP009273/2699735‑2700028
                                                                                                                                                 |                                                                                                                                                    
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCG                                                                                                                                                  >  SRR19973070.1828337/1‑150 (MQ=60)
     AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAAAAACCCCCCGGTAGTAGTCGAAACA                                                                                                                                             >  SRR19973070.2074505/1‑150 (MQ=60)
         CGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                         <  SRR19973070.2451981/150‑1 (MQ=60)
          GGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                        >  SRR19973070.3266595/1‑150 (MQ=60)
             CAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGA                                                                                                                                     >  SRR19973070.2633321/1‑150 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                    >  SRR19973070.2145592/1‑150 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                    >  SRR19973070.2145697/1‑150 (MQ=60)
                   GTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTT                                                                                                                               >  SRR19973070.2364563/1‑150 (MQ=60)
                   GTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTA                                                                                                                               >  SRR19973070.2364473/1‑150 (MQ=60)
                      TCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTG                                                                                                                            <  SRR19973070.542809/150‑1 (MQ=60)
                       CGGCGTGAAGATGCCCAGACGGTCGGCGTTATCGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGC                                                                                                                           <  SRR19973070.3292599/150‑1 (MQ=60)
                       CGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGC                                                                                                                           <  SRR19973070.3292607/150‑1 (MQ=60)
                       CGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGC                                                                                                                           <  SRR19973070.3309244/150‑1 (MQ=60)
                        GGCGTGAAGAGGCCCAGCCGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATCCGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCC                                                                                                                          <  SRR19973070.1244376/150‑1 (MQ=60)
                        GGCGTGAAGAGGCCCAGACGGTCGGCTTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCGGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCC                                                                                                                          <  SRR19973070.1202622/150‑1 (MQ=60)
                          ggttatctttcacttctggtgttagttttaggtgggcctggtactaatggttttctctttgtcgcgcttcctctgtttgacgtttttattacgtttaattcaaaccACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTG                                                                                                                        <  SRR19973070.633218/44‑1 (MQ=60)
                                GATGCCCAGACGGTCGGCGTTATCGGTCGGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGG                                                                                                                  <  SRR19973070.3154225/150‑1 (MQ=60)
                                  TGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGTTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTGGATAATTATTGCCTGCAACGGGC                                                                                                                >  SRR19973070.2403945/1‑150 (MQ=60)
                                     CCAGACGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTT                                                                                                             <  SRR19973070.1968289/150‑1 (MQ=60)
                                     CCAGACGGTCGGCGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTT                                                                                                             <  SRR19973070.2646403/150‑1 (MQ=60)
                                      CAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTATTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTC                                                                                                            >  SRR19973070.2288330/1‑150 (MQ=60)
                                                GCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCT                                                                                                  <  SRR19973070.456222/150‑1 (MQ=60)
                                                GCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCT                                                                                                  <  SRR19973070.454435/150‑1 (MQ=60)
                                                GCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCT                                                                                                  <  SRR19973070.3289396/150‑1 (MQ=60)
                                                      TAGGTCTGCCCGTTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGTATAAGTTAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGC                                                                                            <  SRR19973070.23664/150‑1 (MQ=60)
                                                              CCCGGTACTCATGACTTACACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAT                                                                                    <  SRR19973070.542754/150‑1 (MQ=60)
                                                              CCCGGTACACATGACTTCCACTGGGTCGCCCTTCCGCAGGGGGCGGTTTTTAAGCCGGATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGAGCGCCTTCCGGCGGCGGAAT                                                                                    <  SRR19973070.1794573/150‑1 (MQ=60)
                                                                      TCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCA                                                                            >  SRR19973070.2648989/1‑150 (MQ=60)
                                                                           ACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCGTCCGGCGGCGGAATGTCGCGCACCAGA                                                                       >  SRR19973070.1848693/1‑150 (MQ=60)
                                                                             TTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAAATAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACG                                                                     >  SRR19973070.1078613/1‑150 (MQ=60)
                                                                             TTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACG                                                                     <  SRR19973070.2483145/150‑1 (MQ=60)
                                                                             TTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACG                                                                     <  SRR19973070.1285808/150‑1 (MQ=60)
                                                                               TCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTT                                                                   >  SRR19973070.2702506/1‑150 (MQ=60)
                                                                                  CTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAAAAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTTCCGGCAAAGGGCCGTACGGATAGCCATCCGGCGGCGGAATTTCGCGCCCCAGACGTTGGA                                                                >  SRR19973070.606208/1‑150 (MQ=60)
                                                                                      GTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCACACGTTCGAGAAC                                                            >  SRR19973070.2382384/1‑150 (MQ=60)
                                                                                      GTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGTTAATTAGTGCCTGCAACGGGGCGTCCGGATCGCCTTCCGGCGGCGGGATGTCGCGCCCCAGACGGTCGAGAAC                                                            >  SRR19973070.998729/1‑150 (MQ=60)
                                                                                      GTCGCCCTTACGCAGGGTGCCGTGTTTAATTCGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCAAGAGTCGATAATTAGTGCCTGacacgggccgtccgggtagccataccgccgcggtatgtcgcgccccacaccttcggggac                                                            >  SRR19973070.998685/1‑90 (MQ=60)
                                                                                      GTCGCCCTTACGCAGGGTGCCGTGTTTAATAAGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGAGAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCTTCCGGCGGCGGAAATTCGCGCACCACACGTTCGAGAAC                                                            >  SRR19973070.998727/1‑150 (MQ=60)
                                                                                        CGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTTCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAAGTCGCGCACCCGAAGTTCGAGAACGT                                                          >  SRR19973070.938199/1‑150 (MQ=60)
                                                                                        CGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGCTAGCCTTCCGGCGGCGGAATGTCGCGCACCAGACCTTCGAGCACGT                                                          >  SRR19973070.2061101/1‑150 (MQ=60)
                                                                                        CGCCCTTACGCAGGGTGCCGTGTTTAAAACGGATAAGTGAAACAACGCCCCGGTAGTTGTCGAACCAGGTGTCGATAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCTTACGGCGGCGGcaattcgcgcaccccacggtcgaggacgg                                                          >  SRR19973070.1519159/1‑121 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTAGATAATTATTGCCTGCAACGGGCCTTACGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCC                                                        >  SRR19973070.1807900/1‑150 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAATTTCGCGCACCACACGTTCGAGAACCTCC                                                        >  SRR19973070.3168483/1‑150 (MQ=60)
                                                                                                       TGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTATTCGAAACATGAGTCGATAATTAGTGCCTGCAAACGGCCTTACCGATCGCCTTCCCGCGGCGGAATGTCGCGCACCAGAacttacagaacctccagcacaccaaccc                                           >  SRR19973070.428834/1‑122 (MQ=60)
                                                                                                       TGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACCTCCTGCACACCAACCC                                           >  SRR19973070.2644721/1‑150 (MQ=60)
                                                                                                          CGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGG                                        >  SRR19973070.2586752/1‑150 (MQ=60)
                                                                                                             TTTTAATACGTATATGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTT                                     <  SRR19973070.2942187/150‑1 (MQ=60)
                                                                                                             TTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTT                                     >  SRR19973070.2500455/1‑150 (MQ=60)
                                                                                                             TTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTT                                     >  SRR19973070.2501847/1‑150 (MQ=60)
                                                                                                              TTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTT                                    <  SRR19973070.2197774/150‑1 (MQ=60)
                                                                                                                               AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                   <  SRR19973070.1143439/150‑1 (MQ=60)
                                                                                                                               AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                   <  SRR19973070.1999797/150‑1 (MQ=60)
                                                                                                                               AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                   >  SRR19973070.983553/1‑150 (MQ=60)
                                                                                                                                AACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGC                  >  SRR19973070.2218457/1‑150 (MQ=60)
                                                                                                                                AACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGC                  >  SRR19973070.1080648/1‑150 (MQ=60)
                                                                                                                                  CAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGT                >  SRR19973070.1851353/1‑150 (MQ=60)
                                                                                                                                  CAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCAGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGT                >  SRR19973070.168437/1‑150 (MQ=60)
                                                                                                                                   AACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTC               >  SRR19973070.952007/1‑150 (MQ=60)
                                                                                                                                    ACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCG              <  SRR19973070.1869587/150‑1 (MQ=60)
                                                                                                                                    ACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCG              <  SRR19973070.930684/150‑1 (MQ=60)
                                                                                                                                       CCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTG           >  SRR19973070.2994155/1‑150 (MQ=60)
                                                                                                                                          AGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCG        >  SRR19973070.170755/1‑150 (MQ=60)
                                                                                                                                          AGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCG        >  SRR19973070.170449/1‑150 (MQ=60)
                                                                                                                                             TAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCG     <  SRR19973070.614979/150‑1 (MQ=60)
                                                                                                                                              AGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGA    >  SRR19973070.324513/1‑150 (MQ=60)
                                                                                                                                              AGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGA    >  SRR19973070.324255/1‑150 (MQ=60)
                                                                                                                                               GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT   <  SRR19973070.2252650/150‑1 (MQ=60)
                                                                                                                                                TAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  <  SRR19973070.1576850/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  >  CP009273/2699735‑2700028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑