Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,880 T→A 100% N202Y (AAC→TAC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,8800TA97.2% 450.3 / 2.8 143N202Y (AAC→TAC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (80/59);  total (83/60)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 146 total aligned reads displayed.

AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGT  >  CP009273/2699735‑2700023
                                                                                                                                                 |                                                                                                                                               
agttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTagtagt                                                                                                                                               <  1:3375270/148‑1 (MQ=255)
 gttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTc                                                                                                                                              >  1:3456507/1‑148 (MQ=255)
   tcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGa                                                                                                                                            >  1:258854/1‑148 (MQ=255)
   tcagtGCGGTCAACCTGTGTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGAAACAACGCCCAGGTAGTAGTCGa                                                                                                                                            >  1:258999/1‑148 (MQ=255)
     agtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAAc                                                                                                                                          <  2:1882174/148‑1 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                        >  1:1547725/1‑148 (MQ=255)
         cGGTCAACGTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGGCTGCCCGTTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATg                                                                                                                                      <  1:3113389/148‑1 (MQ=255)
         cGGTCAACCTGTTTCGGCGTTAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATg                                                                                                                                      <  1:3130046/148‑1 (MQ=255)
           gTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAg                                                                                                                                    <  2:1876544/148‑1 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                   >  1:1137379/1‑148 (MQ=255)
              aaCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCg                                                                                                                                 <  2:1453940/148‑1 (MQ=255)
              aaCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCg                                                                                                                                 <  2:1166801/148‑1 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                <  2:1549294/148‑1 (MQ=255)
                 cTGTTTCGGCGTGAAGATGCCCAGACGGGCGGCGTTATAGGTCTGCCCGTTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGTATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATa                                                                                                                              <  1:1632394/148‑1 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                             <  2:3552419/148‑1 (MQ=255)
                     ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTa                                                                                                                          <  2:125228/148‑1 (MQ=255)
                     tggggCGTGAAGATGCCCAGACGGTCGGCTTTATCGGTCGGCCCGTTACTCATGACTTTCACTTGGTCGCCCTTACGCCGGGTGCGTTTTTTAATACGGATATGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTa                                                                                                                          <  1:905733/145‑1 (MQ=255)
                      tCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCGGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAg                                                                                                                         <  1:3414187/148‑1 (MQ=255)
                        ggCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTg                                                                                                                       <  2:2831388/148‑1 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                     <  2:3108548/148‑1 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGCTAATTAGTGCCTg                                                                                                                   >  2:2496783/1‑148 (MQ=255)
                              aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCa                                                                                                                 <  2:3434792/148‑1 (MQ=255)
                                 aTGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACg                                                                                                              <  2:1228249/148‑1 (MQ=255)
                                     ccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                                                                          <  1:2530574/148‑1 (MQ=255)
                                     ccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                                                                          <  1:2530584/148‑1 (MQ=255)
                                       aGACGGTGGGCGTTATTGTTCTGCCGGTTAGTATTGACTTTCAATTTGTCGCCATTACGCAGGGTGCCTTTTTTAATACTGATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCtt                                                                                                        <  1:2792111/148‑1 (MQ=255)
                                       aGACGGTCGGCTTTATAGGTCTGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTCCGCTGGGTGCCTTTTTTAATACGTATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCtt                                                                                                        <  1:1223036/148‑1 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTc                                                                                                       <  1:3158592/148‑1 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTc                                                                                                       <  2:2342069/148‑1 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTcc                                                                                                      >  2:1813147/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTcc                                                                                                      >  2:1597818/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCGTcc                                                                                                      >  1:2190826/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTAGATAAATAGTGCCTTCAACGGGCCTTcc                                                                                                      >  1:2191146/1‑148 (MQ=255)
                                           ggTCGGCGTTATAGGTCTGCCCGTCACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCgg                                                                                                    <  1:1314679/148‑1 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTTACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                                                                   <  2:586790/148‑1 (MQ=255)
                                              cGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCGGCAAAGGGCCTTACGGGTc                                                                                                 >  1:1342969/1‑148 (MQ=255)
                                              cGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTTAAACAACGCCCACGTAGTAGTCGAAACCAGAGTCGATAAATATTTCCTGCAAACGGCCTTCCGGATc                                                                                                 >  1:1340534/1‑148 (MQ=255)
                                                   ttATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCtt                                                                                            <  1:1191705/148‑1 (MQ=255)
                                                   ttATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCtt                                                                                            <  1:1768604/148‑1 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                           >  2:2058908/1‑148 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                           >  2:2058893/1‑148 (MQ=255)
                                                       tggTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcgg                                                                                        <  1:3206826/147‑1 (MQ=255)
                                                       aGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGGCTGCAACGGGCCTTCCGGATCGCCTTCcgg                                                                                        >  2:1539509/1‑148 (MQ=255)
                                                       aGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcgg                                                                                        >  2:3303331/1‑148 (MQ=255)
                                                        ggTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAAGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcggc                                                                                       >  2:417963/1‑148 (MQ=255)
                                                        ggTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAAGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcggc                                                                                       >  2:418073/1‑148 (MQ=255)
                                                           cTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcggcggc                                                                                    <  1:2664934/148‑1 (MQ=255)
                                                            tGCCCGGTACTCATGACTTTCCCTTTGTCGCCCTTACGCAGGGTGCCGTTTGTCATCCGGGTAAGTGAAACCCCGCCGCGGGAGTTGTCGGACCAGGAGTCGGGAGTTGGTGCCTGCCACGGGCCGTCCGGGTCGCCGTGcgggggcg                                                                                   >  1:1832769/1‑148 (MQ=255)
                                                              cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGa                                                                                 <  2:1797873/148‑1 (MQ=255)
                                                               ccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGaa                                                                                <  1:508791/148‑1 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                             <  1:1305279/148‑1 (MQ=255)
                                                                   tACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTc                                                                            <  2:3526390/148‑1 (MQ=255)
                                                                         tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCCTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAAGTCGCGCAc                                                                      >  2:954270/1‑148 (MQ=255)
                                                                         tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAATGTCGCGCAc                                                                      >  2:954299/1‑148 (MQ=255)
                                                                            cTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAg                                                                   >  1:1161232/1‑148 (MQ=255)
                                                                            cTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAg                                                                   >  2:43071/1‑148 (MQ=255)
                                                                            cTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAg                                                                   >  2:43580/1‑148 (MQ=255)
                                                                             tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGa                                                                  <  2:2753525/148‑1 (MQ=255)
                                                                             tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGa                                                                  <  2:2313270/148‑1 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                             >  2:138006/1‑148 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                             >  2:2886545/1‑148 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGGTc                                                             >  2:137929/1‑148 (MQ=255)
                                                                                   tttGTCGCCCTTCCGCAGGGTGCCTTTTTTAATACGGATAATTTAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCg                                                            <  1:2136276/148‑1 (MQ=255)
                                                                                    ttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga                                                           >  2:2866920/1‑148 (MQ=255)
                                                                                     tGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgag                                                          <  2:1257264/148‑1 (MQ=255)
                                                                                      gTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgaga                                                         <  2:3171628/148‑1 (MQ=255)
                                                                                          cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGTGCACCAGACGTTCGAGAACGt                                                     <  2:641012/148‑1 (MQ=255)
                                                                                           ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc                                                    >  2:818441/1‑148 (MQ=255)
                                                                                            cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc                                                   <  1:1843361/148‑1 (MQ=255)
                                                                                             ttACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCt                                                  >  2:1150684/1‑148 (MQ=255)
                                                                                               aCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGc                                                <  2:2676552/148‑1 (MQ=255)
                                                                                                 gCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcac                                              <  2:2500929/148‑1 (MQ=255)
                                                                                                        gCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACg                                       >  2:1564539/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                  >  2:3251467/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                  >  2:2928533/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                  >  2:2879174/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                  >  2:1104755/1‑148 (MQ=255)
                                                                                                              tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                 >  2:1134223/1‑148 (MQ=255)
                                                                                                               ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                >  1:2626691/1‑148 (MQ=255)
                                                                                                               ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                <  2:2969321/148‑1 (MQ=255)
                                                                                                               ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                >  1:2626468/1‑148 (MQ=255)
                                                                                                                tAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtttt                               >  2:1807619/1‑148 (MQ=255)
                                                                                                                  aTACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCg                             >  1:2121822/1‑148 (MQ=255)
                                                                                                                    aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                           >  1:1101398/1‑148 (MQ=255)
                                                                                                                       gATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa                        >  1:1195566/1‑148 (MQ=255)
                                                                                                                       gATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa                        >  1:1185775/1‑148 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                     >  1:603639/1‑148 (MQ=255)
                                                                                                                            gTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcg                   >  1:2210024/1‑148 (MQ=255)
                                                                                                                            gTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcg                   >  1:2210031/1‑148 (MQ=255)
                                                                                                                              gAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa                 >  2:2645809/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg              >  2:3197683/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg              >  1:735735/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg              >  2:1437189/1‑148 (MQ=255)
                                                                                                                                  caacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcgc             >  2:2924651/1‑148 (MQ=255)
                                                                                                                                     cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc          >  1:1443601/1‑148 (MQ=255)
                                                                                                                                     cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc          >  2:2824941/1‑148 (MQ=255)
                                                                                                                                       cccAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCgg        >  1:2537747/1‑148 (MQ=255)
                                                                                                                                          aGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTgg     <  1:1716507/148‑1 (MQ=255)
                                                                                                                                             tagtagTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGt  <  2:2553416/148‑1 (MQ=255)
                                                                                                                                             tagtagTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGt  >  2:851105/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGT  >  CP009273/2699735‑2700023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  >  CP009273/2699731‑2700028
                                                                                                                                                     |                                                                                                                                                    
tttcagttcagtggggaaaacgtgtttgggggtgaagaggcccctcgGGTCGGCGTTTTTGGGCTGCCGGGTACTCATGATTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATCCGTATATGTGAAACAACGCCCAGGTAGTA                                                                                                                                                      <  SRR19973067.3415762/103‑1 (MQ=60)
 TTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAG                                                                                                                                                     >  SRR19973067.1463743/1‑150 (MQ=60)
 TTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAG                                                                                                                                                     >  SRR19973067.1463672/1‑150 (MQ=60)
   CATTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTC                                                                                                                                                   <  SRR19973067.296777/150‑1 (MQ=60)
   CAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTC                                                                                                                                                   >  SRR19973067.3478489/1‑150 (MQ=60)
    AGTTCCGTGCGGTCAACGTTTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCG                                                                                                                                                  <  SRR19973067.3396725/150‑1 (MQ=60)
     GTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGA                                                                                                                                                 >  SRR19973067.260624/1‑150 (MQ=60)
     GTTCAGTGCGGTCAACCTGTGTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGAAACAACGCCCAGGTAGTAGTCGA                                                                                                                                                 >  SRR19973067.260770/1‑150 (MQ=60)
         AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                             >  SRR19973067.1558249/1‑150 (MQ=60)
             CGGTCAACGTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGGCTGCCCGTTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                         <  SRR19973067.3133180/150‑1 (MQ=60)
             CGGTCAACCTGTTTCGGCGTTAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                         <  SRR19973067.3149936/150‑1 (MQ=60)
             CGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                         <  SRR19973067.3149940/150‑1 (MQ=60)
              GGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                        >  SRR19973067.1145033/1‑150 (MQ=60)
                 CAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATAAGGATAAGTGAAACAAAGCCCAGGTAGTAGTCGAACCcagaggcgt                                                                                                                                     >  SRR19973067.616305/1‑141 (MQ=60)
                     CTGTTTCGGCGTGAAGATGCCCAGACGGGCGGCGTTATAGGTCTGCCCGTTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGTATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAT                                                                                                                                 <  SRR19973067.1643624/150‑1 (MQ=60)
                         tggGGCGTGAAGATGCCCAGACGGTCGGCTTTATCGGTCGGCCCGTTACTCATGACTTTCACTTGGTCGCCCTTACGCCGGGTGCGTTTTTTAATACGGATATGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGT                                                                                                                             <  SRR19973067.911726/147‑1 (MQ=60)
                          TCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCGGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTG                                                                                                                            <  SRR19973067.3435923/150‑1 (MQ=60)
                              CGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTG                                                                                                                        <  SRR19973067.2590986/150‑1 (MQ=60)
                                   AGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCATTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACG                                                                                                                   >  SRR19973067.1169358/1‑150 (MQ=60)
                                   AGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCATTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACG                                                                                                                   >  SRR19973067.2953731/1‑150 (MQ=60)
                                         CCAGACGGTGGGCTTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTT                                                                                                             <  SRR19973067.2547164/150‑1 (MQ=60)
                                         CCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTT                                                                                                             <  SRR19973067.2547154/150‑1 (MQ=60)
                                           agacggtgggcgttattgttctgccggttagtatTGACTTTCAATTTGTCGCCATTACGCAGGGTGCCTTTTTTAATACTGATAAGTCAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCC                                                                                                           <  SRR19973067.2810133/116‑1 (MQ=60)
                                           AGACGGTCGGCTTTATAGGTCTGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTCCGCTGGGTGCCTTTTTTAATACGTATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCC                                                                                                           <  SRR19973067.1231184/150‑1 (MQ=60)
                                           AGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCGTCC                                                                                                           >  SRR19973067.2205448/1‑150 (MQ=60)
                                           AGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTAGATAAATAGTGCCTTCAACGGGCCTTCC                                                                                                           >  SRR19973067.2205772/1‑150 (MQ=60)
                                            GACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCCCTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCG                                                                                                          <  SRR19973067.3178607/150‑1 (MQ=60)
                                             ACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGCACCACCGCCCAGGTCGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGG                                                                                                         <  SRR19973067.3511695/150‑1 (MQ=60)
                                               GGTCGGCGTTATAGGTCTGCCCGTCACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGAT                                                                                                       <  SRR19973067.1323659/150‑1 (MQ=60)
                                                GTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCGGCAAAGGGCCTTACGGGTC                                                                                                      >  SRR19973067.1352156/1‑150 (MQ=60)
                                                GTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTTAAACAACGCCCACGTAGTAGTCGAAACCAGAGTCGATAAATATTTCCTGCAAACGGCCTTCCGGATC                                                                                                      >  SRR19973067.1349702/1‑150 (MQ=60)
                                                    GCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCT                                                                                                  <  SRR19973067.999684/150‑1 (MQ=60)
                                                       TTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCC                                                                                               <  SRR19973067.1199534/150‑1 (MQ=60)
                                                       TTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCC                                                                                               <  SRR19973067.1780617/150‑1 (MQ=60)
                                                       TTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCC                                                                                               <  SRR19973067.1780877/150‑1 (MQ=60)
                                                           agttcgggCCGGTACTCATGACTTTCACTTTGTCGCCGTTCCGCTGGGTGGCGTTTTTAATCCTCATACTTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCG                                                                                           <  SRR19973067.3329188/142‑1 (MQ=60)
                                                           TGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCG                                                                                           <  SRR19973067.3227039/150‑1 (MQ=60)
                                                               CTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGG                                                                                       <  SRR19973067.2682062/150‑1 (MQ=60)
                                                                   CCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATG                                                                                   <  SRR19973067.512259/150‑1 (MQ=60)
                                                                      GTACTCATGACTTTCACTTTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCG                                                                                <  SRR19973067.1314181/150‑1 (MQ=60)
                                                                         CTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTAGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCATCCGGCGGCGGCATGTCGCGC                                                                             >  SRR19973067.172201/1‑150 (MQ=60)
                                                                             TGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCA                                                                         <  SRR19973067.267873/150‑1 (MQ=60)
                                                                              GACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAG                                                                        >  SRR19973067.1168962/1‑150 (MQ=60)
                                                                                 TTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACG                                                                     <  SRR19973067.1263690/150‑1 (MQ=60)
                                                                                       TTTGTCGCCCTTCCGCAGGGTGCCTTTTTTAATACGGATAATTTAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAG                                                               <  SRR19973067.2150546/150‑1 (MQ=60)
                                                                                            CGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACCTTCGAGAACGT                                                          >  SRR19973067.2617875/1‑150 (MQ=60)
                                                                                             GCCCTTACGCAGGGTGCCGTTTTTAATACGGGTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCTGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTC                                                         >  SRR19973067.700117/1‑150 (MQ=60)
                                                                                                CTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG                                                      <  SRR19973067.1855933/150‑1 (MQ=60)
                                                                                                          GTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACG                                            <  SRR19973067.1326179/150‑1 (MQ=60)
                                                                                                           TGCCGTTTTTAATAAGGATAAGTGAAACAACGCCCACGTAGTAGTCGAAACATGATTCGATAAATAGTGCCTGCAACGGGCCTTACGGATCGCCTTACCGCGGCGGAATTTCGCGCACCACACGTTCGAGAACGTCCAGCACACCAACCC                                           >  SRR19973067.1238989/1‑150 (MQ=60)
                                                                                                            GCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCC                                          <  SRR19973067.1435370/150‑1 (MQ=60)
                                                                                                              CGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGG                                        >  SRR19973067.2195335/1‑150 (MQ=60)
                                                                                                                 TTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTT                                     >  SRR19973067.2643716/1‑150 (MQ=60)
                                                                                                                 TTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTT                                     >  SRR19973067.2643491/1‑150 (MQ=60)
                                                                                                                    TAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCG                                  >  SRR19973067.333668/1‑150 (MQ=60)
                                                                                                                    TAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCG                                  >  SRR19973067.2135981/1‑150 (MQ=60)
                                                                                                                      ATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCT                                >  SRR19973067.1108890/1‑150 (MQ=60)
                                                                                                                         CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                             >  SRR19973067.1946364/1‑150 (MQ=60)
                                                                                                                         CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                             >  SRR19973067.1203405/1‑150 (MQ=60)
                                                                                                                         CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                             >  SRR19973067.1203126/1‑150 (MQ=60)
                                                                                                                         CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                             >  SRR19973067.1193591/1‑150 (MQ=60)
                                                                                                                            ATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAG                          >  SRR19973067.607473/1‑150 (MQ=60)
                                                                                                                              AAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCG                        >  SRR19973067.2224764/1‑150 (MQ=60)
                                                                                                                              AAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCG                        >  SRR19973067.2224757/1‑150 (MQ=60)
                                                                                                                                GTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCA                      >  SRR19973067.2219295/1‑150 (MQ=60)
                                                                                                                                   AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                   >  SRR19973067.740843/1‑150 (MQ=60)
                                                                                                                                       AACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTC               >  SRR19973067.1453300/1‑150 (MQ=60)
                                                                                                                                         CGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGG             >  SRR19973067.2554363/1‑150 (MQ=60)
                                                                                                                                           CCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTG           >  SRR19973067.2230063/1‑150 (MQ=60)
                                                                                                                                              AGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCG        <  SRR19973067.1728270/150‑1 (MQ=60)
                                                                                                                                                    TAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  <  SRR19973067.1871206/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG  >  CP009273/2699731‑2700028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑