Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,880 T→A 100% N202Y (AAC→TAC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,8800TA99.2% 553.9 / ‑4.2 178N202Y (AAC→TAC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (101/75);  total (103/75)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024
                                                                                                                                                 |                                                                                                                                                
    cagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGCCGGTCGGCGTTATAGGTCTGCCCGGTACTCTTGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGaa                                                                                                                                            <  1:1100009/148‑1 (MQ=255)
     agtGCGGACAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAAc                                                                                                                                           <  1:634866/148‑1 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         >  1:2932426/1‑148 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         >  1:1891111/1‑148 (MQ=255)
          ggTCACCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:2124494/148‑1 (MQ=255)
          ggTCAACCTGTTTGGGCTTGAAGTGGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTCATGAATTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATCCGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:356332/148‑1 (MQ=255)
           gTCACCCTGTGTCGGCGTGAAGAGGCCCCGACGGTGGGGTTTATCGGTCTGCCCGTTACTCTTGACTTACAGTTTGTCCCCCTTACGCCGGGTGCCGTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAg                                                                                                                                     <  1:307499/148‑1 (MQ=255)
           gTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGCTAAGTGAAACAACGCCCCGGTAGTAGTCGCACCATGAg                                                                                                                                     >  1:641584/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                    >  1:1826828/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                    >  2:2988826/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGGAGTCGAACCAAGAGt                                                                                                                                    >  1:2692006/1‑148 (MQ=255)
             cAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTc                                                                                                                                   >  1:2996116/1‑148 (MQ=255)
              aaCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCg                                                                                                                                  >  2:2969099/1‑148 (MQ=255)
               cccTGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCTTTATCGGTCTGCCCGGTACTCATGACTTACACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATCCGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 <  1:728114/147‑1 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 <  1:2239818/148‑1 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                <  2:1674488/148‑1 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:702851/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:1870830/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:3305068/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:3154931/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:3154929/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGGTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:1564996/1‑148 (MQ=255)
                 cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAAAGCCCAGGTAGTATTCGAACCATGatgagata                                                                                                                               >  1:3418781/1‑141 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACTGATAATTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              <  1:3352144/148‑1 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              <  2:921504/148‑1 (MQ=255)
                    tttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAtt                                                                                                                            >  1:1179219/1‑148 (MQ=255)
                     ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTa                                                                                                                           >  1:592890/1‑148 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      <  1:2930942/148‑1 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      <  2:2017978/148‑1 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGcc                                                                                                                      >  1:2174638/1‑148 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:113419/148‑1 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:512450/148‑1 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTGTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:2277172/148‑1 (MQ=255)
                              aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCa                                                                                                                  <  2:1360212/148‑1 (MQ=255)
                                gATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATACGGATAATTGAAACAACCCCCAGGTAGTAGTCGAACCATGATTCGATTATTATTGGCTTCAAc                                                                                                                >  1:21091/1‑148 (MQ=255)
                                gATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAAAAACCCCCAGGTAGTAGTCGAACCCTGAGTCGATAATTAGTGCCTGCAAc                                                                                                                >  1:386358/1‑148 (MQ=255)
                                 aTGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACg                                                                                                               >  1:1741501/1‑148 (MQ=255)
                                  tGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATAGTGCCTGCAACgg                                                                                                              >  1:2746695/1‑148 (MQ=255)
                                    cccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACGTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGc                                                                                                            <  2:3235917/148‑1 (MQ=255)
                                       aGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCtt                                                                                                         >  2:2477253/1‑148 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTc                                                                                                        >  1:1713604/1‑148 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAATGAAACAACGCCCACGGAGTAGTAGAACCAAGAGTCGATAAATTTTTCCGGCAACCGGCCGTc                                                                                                        >  1:912745/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTcc                                                                                                       >  2:2425185/1‑148 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTCGATAATTATTTCCTGCAACGGGccataccga                                                                                                    >  1:2766497/1‑141 (MQ=255)
                                               ggCTTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGGGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCg                                                                                                 <  1:321817/148‑1 (MQ=255)
                                                   ttATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCtt                                                                                             <  2:2052396/148‑1 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            >  2:2663173/1‑148 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            >  2:3142080/1‑148 (MQ=255)
                                                     ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTcc                                                                                           <  1:2643627/148‑1 (MQ=255)
                                                     ataGGTCTGCCCGGTACTCATGACTTTCACTGTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTcc                                                                                           <  1:2086292/148‑1 (MQ=255)
                                                                 ggTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATg                                                                               <  1:1400834/148‑1 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              >  1:1462696/1‑148 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              <  2:333372/148‑1 (MQ=255)
                                                                  gTACTCATGACTGTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACACCGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              <  2:2788104/148‑1 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGCTAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            >  1:2276938/1‑148 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACCGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAtttac                                                                            >  1:511759/1‑144 (MQ=255)
                                                                     cTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTTATAAGGTTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTTGCTTCAACGGGCCGTGCGGATCGGCCTACGGCGGCGGGATTTcgc                                                                           >  1:617642/1‑148 (MQ=255)
                                                                       cATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAAAGTCgcgc                                                                         >  2:2759924/1‑148 (MQ=255)
                                                                         tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAc                                                                       >  1:3422652/1‑148 (MQ=255)
                                                                           aCTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCa                                                                     >  2:3059620/1‑148 (MQ=255)
                                                                             tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAAGTCGCGCACCAGa                                                                   >  1:1282502/1‑148 (MQ=255)
                                                                              ttCACTTTGTCGCCCTTACGCAGGGTGCCGTTGTTAATAAGGATAATTGAAACAAAGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACCGGCCATCCCGATCGCCTTACCGCGGCCGAATGTCCCGCAACAAAc                                                                  >  1:2060182/1‑148 (MQ=255)
                                                                                 aCTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGtt                                                               >  2:1792450/1‑148 (MQ=255)
                                                                                   tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCg                                                             <  1:328034/148‑1 (MQ=255)
                                                                                    ttGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga                                                            <  1:32266/148‑1 (MQ=255)
                                                                                      gTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgaga                                                          <  2:1317628/148‑1 (MQ=255)
                                                                                       tCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGaa                                                         >  1:2301609/1‑148 (MQ=255)
                                                                                          cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTATTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCGGATCGCCATCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt                                                      >  2:2244735/1‑148 (MQ=255)
                                                                                          cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt                                                      <  1:2317273/148‑1 (MQ=255)
                                                                                           ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc                                                     <  1:1410587/148‑1 (MQ=255)
                                                                                            cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc                                                    >  1:867681/1‑148 (MQ=255)
                                                                                              tACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTg                                                  >  2:338614/1‑148 (MQ=255)
                                                                                              tACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTAGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCACACGTTCGAGAACGTACTg                                                  >  2:338538/1‑148 (MQ=255)
                                                                                               aCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGc                                                 >  2:1090131/1‑148 (MQ=255)
                                                                                                cGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGca                                                >  2:1374499/1‑148 (MQ=255)
                                                                                                  cAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca                                              >  2:2178032/1‑148 (MQ=255)
                                                                                                  cAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca                                              >  2:410998/1‑148 (MQ=255)
                                                                                                      gTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCaa                                          <  1:1539985/148‑1 (MQ=255)
                                                                                                          cGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc                                      >  2:2262751/1‑148 (MQ=255)
                                                                                                            tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg                                    >  1:2684452/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                   >  1:2838386/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                   >  1:1679019/1‑148 (MQ=255)
                                                                                                              tttAATAGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  1:735636/148‑1 (MQ=255)
                                                                                                               ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                 <  1:3105006/148‑1 (MQ=255)
                                                                                                                     cGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTg                           <  1:1717523/148‑1 (MQ=255)
                                                                                                                       gATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa                         <  1:1775235/148‑1 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCGTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                      >  1:868987/1‑148 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                      >  2:3414281/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   >  2:3231306/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   <  2:2965623/148‑1 (MQ=255)
                                                                                                                               aaaCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc                 >  1:2884540/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg               >  2:1125471/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg               >  1:1206694/1‑148 (MQ=255)
                                                                                                                                     cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc           >  1:619034/1‑148 (MQ=255)
                                                                                                                                      gCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCg          <  2:3418781/148‑1 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  1:6158/148‑1 (MQ=255)
                                                                                                                                         cAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTg       <  2:1256402/148‑1 (MQ=255)
                                                                                                                                           ggTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc     <  1:1870830/148‑1 (MQ=255)
                                                                                                                                             tagtagTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGt   >  1:1087742/1‑148 (MQ=255)
                                                                                                                                              agtagtCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTc  >  2:804098/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT  >  CP009273/2699735‑2700027
                                                                                                                                                 |                                                                                                                                                   
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCG                                                                                                                                                 <  SRR19973065.273221/150‑1 (MQ=60)
    CAGTGGGGTCAACCTGTTTCGGCGTGAAGATGCCCAGCCGGTCGGCGTTATAGGTCTGCCCGGTACTCTTGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACC                                                                                                                                             <  SRR19973065.1107239/150‑1 (MQ=60)
     AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                            >  SRR19973065.2950216/1‑150 (MQ=60)
     AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                            >  SRR19973065.1903544/1‑150 (MQ=60)
     AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGGAGTAGTCGAACCA                                                                                                                                            >  SRR19973065.3296723/1‑150 (MQ=60)
     AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGGAGTAGTCGAACCA                                                                                                                                            >  SRR19973065.113713/1‑150 (MQ=60)
     AGTGCGGACAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                            <  SRR19973065.639009/150‑1 (MQ=60)
     AGTGCGGACAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATACGTATACGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                            <  SRR19973065.638974/150‑1 (MQ=60)
     AGGGCGGTCAACCGGTTTCGGCGTGAAGATGCCCAGCCGGTGGGCGTTATTGGTCGCCCGGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATATGTGAAACAACGCCCAGGTAGTAGTCGAACCA                                                                                                                                            <  SRR19973065.639073/150‑1 (MQ=60)
         CGGTCAACCTTTTTCGGCGTGAAGAGGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                        <  SRR19973065.2852716/150‑1 (MQ=60)
         CGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGCTAAGTGAAACAACGCCCCGGTAGTAGTCGCACCATGAG                                                                                                                                        >  SRR19973065.645795/1‑150 (MQ=60)
          GGTCACCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                       <  SRR19973065.2138167/150‑1 (MQ=60)
          GGTCAACCTGTTTGGGCTTGAAGTGGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTCATGAATTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATCCGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                       <  SRR19973065.358841/150‑1 (MQ=60)
          GGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                       >  SRR19973065.1838863/1‑150 (MQ=60)
          GGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                       >  SRR19973065.1838852/1‑150 (MQ=60)
          GGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGGAGTCGAACCAAGAGT                                                                                                                                       >  SRR19973065.2708722/1‑150 (MQ=60)
           GTCACCCTGTGTCGGCGTGAAGAGGCCCCGACGGTGGGGTTTATCGGTCTGCCCGTTACTCTTGACTTACAGTTTGTCCCCCTTACGCCGGGTGCCGTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTC                                                                                                                                      <  SRR19973065.309548/150‑1 (MQ=60)
           GTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTATTCGAAACATGAGTC                                                                                                                                      >  SRR19973065.3014387/1‑150 (MQ=60)
           GTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTC                                                                                                                                      >  SRR19973065.3014467/1‑150 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                   >  SRR19973065.707525/1‑150 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                   >  SRR19973065.3174065/1‑150 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                   <  SRR19973065.592105/150‑1 (MQ=60)
              AACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAT                                                                                                                                   >  SRR19973065.3174067/1‑150 (MQ=60)
               CCCTGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCTTTATCGGTCTGCCCGGTACTCATGACTTACACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATCCGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATA                                                                                                                                  <  SRR19973065.732991/150‑1 (MQ=60)
               ACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAAAGCCCAGGTAGTATTCGAACCATGAtgagata                                                                                                                                  >  SRR19973065.3439667/1‑143 (MQ=60)
               ACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATA                                                                                                                                  <  SRR19973065.2254056/150‑1 (MQ=60)
                  tgtttcggagttaagatgcccagagggtgggcgttattgttgtgcccgttaatcttcaatttctctgggtcgcacttccgatgggtgcgtTTTTTAATACGTATTAGTCAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                               <  SRR19973065.1257923/60‑1 (MQ=37)
                  TGTTTGGGCGTGAAGATGCCCTGACGGTCGGCGTTATAGGTCTGCCCGTTACTATTGACTTTCAGTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                               <  SRR19973065.3372628/150‑1 (MQ=60)
                  TGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACTGATAATTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                               <  SRR19973065.3372629/150‑1 (MQ=60)
                  TGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                               <  SRR19973065.1766070/150‑1 (MQ=60)
                  TGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                               >  SRR19973065.1186697/1‑150 (MQ=60)
                   GTTTCGGCGTTAAGAGGCCCAGACGTTCGGCTTTATCGGTCGGCCCGTTACTCATGACTTGCACTTGGGCGCCCTTCCGCTGGGTGCGTTTTTTAATCCGGATATGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTA                                                                                                                              <  SRR19973065.618871/150‑1 (MQ=53)
                   GTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTA                                                                                                                              >  SRR19973065.3023804/1‑150 (MQ=60)
                   GTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTA                                                                                                                              >  SRR19973065.596553/1‑150 (MQ=60)
                      TCGGCGTGAAGATGCCCAGCCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTG                                                                                                                           <  SRR19973065.2135825/150‑1 (MQ=60)
                        GGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGCC                                                                                                                         >  SRR19973065.2188589/1‑150 (MQ=60)
                          CGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTG                                                                                                                       <  SRR19973065.2948729/150‑1 (MQ=60)
                              AAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATACGGATAATTGAAACAACCCCCAGGTAGTAGTCGAACCATGATTCGATTATTATTGGCTTCAAC                                                                                                                   >  SRR19973065.21377/1‑150 (MQ=60)
                              AAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAAC                                                                                                                   <  SRR19973065.2218750/150‑1 (MQ=60)
                              AAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAAAAACCCCCAGGTAGTAGTCGAACCCTGAGTCGATAATTAGTGCCTGCAAC                                                                                                                   >  SRR19973065.389054/1‑150 (MQ=60)
                              AAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATATTGCCTGCAAC                                                                                                                   >  SRR19973065.391514/1‑150 (MQ=60)
                               AGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACG                                                                                                                  >  SRR19973065.1752997/1‑150 (MQ=60)
                                GATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATAGTGCCTGCAACGG                                                                                                                 >  SRR19973065.2763786/1‑150 (MQ=60)
                                  TGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGC                                                                                                               >  SRR19973065.229551/1‑150 (MQ=60)
                                      CAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTC                                                                                                           >  SRR19973065.1724838/1‑150 (MQ=60)
                                      CAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTC                                                                                                           >  SRR19973065.1895406/1‑150 (MQ=60)
                                      CAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAAAGGGCCTTC                                                                                                           >  SRR19973065.1724998/1‑150 (MQ=60)
                                      CAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAATGAAACAACGCCCACGGAGTAGTAGAACCAAGAGTCGATAAatttttccggcaaccggccgtc                                                                                                           >  SRR19973065.918692/1‑128 (MQ=60)
                                       AGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTAC                                                                                                          >  SRR19973065.3488768/1‑150 (MQ=60)
                                          CGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTCGATAATTATTTCCTGCAACGGGCCataccga                                                                                                       >  SRR19973065.2783692/1‑143 (MQ=60)
                                            GTCGGCGTTATCGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATC                                                                                                     <  SRR19973065.689718/150‑1 (MQ=60)
                                            GTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATC                                                                                                     <  SRR19973065.2461895/150‑1 (MQ=60)
                                               GGCTTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGGGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCC                                                                                                  <  SRR19973065.324043/150‑1 (MQ=60)
                                                     ATAGGTCTGCCCGTTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGG                                                                                            <  SRR19973065.2099769/150‑1 (MQ=60)
                                                     ATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGG                                                                                            <  SRR19973065.2659936/150‑1 (MQ=60)
                                                     ATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGG                                                                                            <  SRR19973065.2660934/150‑1 (MQ=60)
                                                     ATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGG                                                                                            <  SRR19973065.2099652/150‑1 (MQ=60)
                                                     ATAGGTCTGCCCGGTACTCATGACTTTCACTGTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGG                                                                                            <  SRR19973065.2099667/150‑1 (MQ=60)
                                                          tcggccgggtactcttgactttctgtgtgacgcgCTTACGCTGGTTGCCGTTTTTAATACGGATATGTGAAACCACGCCCAGGTAGTAGTCGACCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCG                                                                                       <  SRR19973065.1854276/116‑1 (MQ=60)
                                                                CNGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGT                                                                                 >  SRR19973065.1472094/1‑150 (MQ=60)
                                                                 GGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTC                                                                                <  SRR19973065.1410019/150‑1 (MQ=60)
                                                                  GTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGCTAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCG                                                                               >  SRR19973065.2291303/1‑150 (MQ=60)
                                                                  GTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACCGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATttac                                                                               >  SRR19973065.515149/1‑146 (MQ=60)
                                                                   TACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTTATAAGGTTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAtttgcttcaacgggccgtgcggatcggcctacggcggcgggatttcgc                                                                              >  SRR19973065.621607/1‑102 (MQ=60)
                                                                       CATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAC                                                                          >  SRR19973065.2906963/1‑150 (MQ=60)
                                                                       CATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAC                                                                          >  SRR19973065.3443552/1‑150 (MQ=60)
                                                                        ATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACC                                                                         >  SRR19973065.2320526/1‑150 (MQ=60)
                                                                          GACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAAAAACGCCCCGGTAGTAGTCGAACCATGAGGCGATAAATAGTTGCTGCAACGGGCCGTCCGGATCGCCTTCCCGCGGCGGAATGTCGCGCACCAC                                                                       >  SRR19973065.622187/1‑150 (MQ=60)
                                                                           ACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAAGTCGCGCACCAGA                                                                      >  SRR19973065.1290965/1‑150 (MQ=60)
                                                                           ACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGAAACAACGACCAGGTAGTATTCGAACCATGAGTCGATAATTATTTCCTGCAACGGGCCGTCCGGATCGCCTTCCGGCGGCGGACTGTCGggccccagg                                                                      >  SRR19973065.1290924/1‑141 (MQ=60)
                                                                            CTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTGTTAATAAGGATAATTGAAACAAAGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACCGGCCATCCCGATCGCCTTACCGCGGCCGAATGTCCCGCAACAAAC                                                                     >  SRR19973065.2073326/1‑150 (MQ=60)
                                                                              TTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGT                                                                   >  SRR19973065.3241371/1‑150 (MQ=60)
                                                                                   TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAG                                                              <  SRR19973065.1375339/150‑1 (MQ=60)
                                                                                   TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAG                                                              <  SRR19973065.330321/150‑1 (MQ=60)
                                                                                    TTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGA                                                             <  SRR19973065.32669/150‑1 (MQ=60)
                                                                                     TGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAA                                                            >  SRR19973065.2316088/1‑150 (MQ=60)
                                                                                      GTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGGAAATTATTGCCAGCAACGGGCCGTCCGGATCGCCGTCCGGCGGCGGAATGTCGCGCACCCGACGTTCGAGAAC                                                           >  SRR19973065.383097/1‑150 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCC                                                       >  SRR19973065.873245/1‑150 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCC                                                       >  SRR19973065.872745/1‑150 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCC                                                       >  SRR19973065.873287/1‑150 (MQ=60)
                                                                                          CCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCC                                                       <  SRR19973065.2331799/150‑1 (MQ=60)
                                                                                           CCTTCCGCTGGGTGCGGTTTTTAATTCGGATATGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCT                                                      <  SRR19973065.1551784/150‑1 (MQ=60)
                                                                                           CCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCT                                                      <  SRR19973065.1419812/150‑1 (MQ=60)
                                                                                            CTTACGCTGGGTGCCTTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG                                                     <  SRR19973065.1542046/150‑1 (MQ=60)
                                                                                                      GTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACG                                           <  SRR19973065.1550158/150‑1 (MQ=60)
                                                                                                          CGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGG                                       >  SRR19973065.2701100/1‑150 (MQ=60)
                                                                                                           GTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGT                                      >  SRR19973065.2855859/1‑150 (MQ=60)
                                                                                                           GTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGT                                      >  SRR19973065.1690243/1‑150 (MQ=60)
                                                                                                            TTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTT                                     >  SRR19973065.734237/1‑150 (MQ=60)
                                                                                                            TTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTT                                     >  SRR19973065.1428111/1‑150 (MQ=60)
                                                                                                              tttaatacgtataatgtaacccACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTT                                   <  SRR19973065.908803/128‑1 (MQ=60)
                                                                                                              TTTAATAGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTT                                   <  SRR19973065.740553/150‑1 (MQ=60)
                                                                                                               TTAATAGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTC                                  <  SRR19973065.3124016/150‑1 (MQ=60)
                                                                                                               TTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTC                                  <  SRR19973065.3123806/150‑1 (MQ=60)
                                                                                                               TTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTC                                  <  SRR19973065.3123951/150‑1 (MQ=60)
                                                                                                                     CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                            <  SRR19973065.1728940/150‑1 (MQ=60)
                                                                                                                     CGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAA                            <  SRR19973065.1728920/150‑1 (MQ=60)
                                                                                                                       GATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACA                          <  SRR19973065.1786845/150‑1 (MQ=60)
                                                                                                                        ATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCGTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAG                         >  SRR19973065.874557/1‑150 (MQ=60)
                                                                                                                          AAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCG                       <  SRR19973065.3180228/150‑1 (MQ=60)
                                                                                                                            GTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCA                     <  SRR19973065.2180108/150‑1 (MQ=60)
                                                                                                                             TGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAC                    >  SRR19973065.2902131/1‑150 (MQ=60)
                                                                                                                             TGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAC                    >  SRR19973065.2902183/1‑150 (MQ=60)
                                                                                                                               AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                  >  SRR19973065.1214457/1‑150 (MQ=60)
                                                                                                                               AAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCG                  >  SRR19973065.1214456/1‑150 (MQ=60)
                                                                                                                                   AACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTC              >  SRR19973065.623014/1‑150 (MQ=60)
                                                                                                                                        CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG         <  SRR19973065.6253/150‑1 (MQ=60)
                                                                                                                                        CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG         >  SRR19973065.2773857/1‑150 (MQ=60)
                                                                                                                                        CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG         <  SRR19973065.3435577/150‑1 (MQ=60)
                                                                                                                                           GGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGT      <  SRR19973065.1883147/150‑1 (MQ=60)
                                                                                                                                           GGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGT      >  SRR19973065.1094913/1‑150 (MQ=60)
                                                                                                                                             TAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCG    >  SRR19973065.1827026/1‑150 (MQ=60)
                                                                                                                                               GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT  <  SRR19973065.2836259/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT  >  CP009273/2699735‑2700027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑