Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,880 T→A 100% N202Y (AAC→TAC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,8800TA97.0% 509.1 / 4.9 164N202Y (AAC→TAC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base A (82/77);  total (85/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 170 total aligned reads displayed.

AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024
                                                                                                                                                 |                                                                                                                                                
agttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTagtagt                                                                                                                                                >  1:1419613/1‑148 (MQ=255)
 gttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTc                                                                                                                                               <  2:2620915/148‑1 (MQ=255)
 gttcagtGCGGTCAACCTGTTTCGGCGTCAATATGCCCAGACGGTCGGCGTTATAGGTCTGACCCGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGATTAATAAGCATAAGTGAAACAAAGCCCAGGTACTAGTc                                                                                                                                               >  1:1036841/1‑148 (MQ=255)
  ttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCg                                                                                                                                              >  2:3015074/1‑148 (MQ=255)
   tcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGa                                                                                                                                             <  2:2532477/148‑1 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGATGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGTGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         <  2:1203931/148‑1 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTGATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGAGAAGTGAAACAACACCCACGTATTAGTCGAACCa                                                                                                                                         >  1:2417923/1‑148 (MQ=255)
         cGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATg                                                                                                                                       <  1:2558130/148‑1 (MQ=255)
          ggTCAACCTGTTTGGGCGTGAAGAGGCCCAGACGGGCGGGTTTATAGGTCGGCCCGGTACTCATGACTTCCACTTTGGCGCCCTTACGCAGGGTGCGGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:446628/148‑1 (MQ=255)
          ggTCAACCTGTTTCGGCGTTAAGATGCCCAGACGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATATGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:446668/148‑1 (MQ=255)
           gTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGGGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAg                                                                                                                                     <  1:1993779/148‑1 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAACCCCCCGGTAGTAGTCGAAACAAGAGTCgc                                                                                                                                 >  1:614343/1‑147 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 >  2:3484240/1‑148 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 >  2:1242408/1‑148 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 >  2:2935043/1‑148 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCTGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              <  2:3192795/148‑1 (MQ=255)
                    tttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAtt                                                                                                                            >  1:2583460/1‑148 (MQ=255)
                      tCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAg                                                                                                                          <  2:559726/148‑1 (MQ=255)
                      tCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAg                                                                                                                          <  2:559728/148‑1 (MQ=255)
                             gAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGc                                                                                                                   <  1:1790818/148‑1 (MQ=255)
                             gAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGTAACAACGCCCAGGTAGTAGTCGAACCCTGAGTCGCTAATTAGTGCCAGc                                                                                                                   >  1:2119602/1‑148 (MQ=255)
                               aGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCaa                                                                                                                 >  2:3479109/1‑148 (MQ=255)
                               aGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAAACATGAGTCGATAATTAGTGCCTGCaa                                                                                                                 >  2:306993/1‑148 (MQ=255)
                                 ttGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTAATGATTTTCTCTTTGTCGCCCTTCCGCAGGGTGCCTTTTTTAATACGTATATGTTAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACg                                                                                                               <  1:81018/147‑1 (MQ=255)
                                     ccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                                                                           >  1:2067355/1‑148 (MQ=255)
                                      cAGAGGGGCGGCTTTATCGGTCGGCCCGGTACTCATGACTTTCACTTTGGCGCCCTTCCGCGGGGTGCCGTTTTTAATCCGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCt                                                                                                          <  1:920127/148‑1 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTcc                                                                                                       >  2:1077378/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTAGAACCATTAGTAGATAATTATTTGCAGCAACCGGCCGTcc                                                                                                       >  1:676365/1‑148 (MQ=255)
                                          cGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCg                                                                                                      <  1:2965189/148‑1 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                                                                    <  1:274668/148‑1 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                                                                    <  1:275160/148‑1 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                                                                    <  2:111984/148‑1 (MQ=255)
                                                gCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGTTAAGTGAAACAACGCCAAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGTCTGCAACGGGCCTTCAGGATCGc                                                                                                <  2:259237/148‑1 (MQ=255)
                                                gCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGc                                                                                                <  2:23737/148‑1 (MQ=255)
                                                 cGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGcc                                                                                               <  2:1419613/148‑1 (MQ=255)
                                                 cGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGcc                                                                                               >  2:1939169/1‑148 (MQ=255)
                                                 cGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGcc                                                                                               >  2:1939232/1‑148 (MQ=255)
                                                 cGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGcc                                                                                               <  1:1834461/148‑1 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCGTCCGGATCGCCTTc                                                                                            >  2:230683/1‑148 (MQ=255)
                                                       aGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCcgg                                                                                         <  1:2850833/148‑1 (MQ=255)
                                                              cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGa                                                                                  <  2:2443977/148‑1 (MQ=255)
                                                              cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGa                                                                                  >  2:2901891/1‑148 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              >  2:2227187/1‑148 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              >  2:2916956/1‑148 (MQ=255)
                                                                  gTACTCATGACTTACACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              <  2:3011568/148‑1 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            >  2:2914584/1‑148 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACCGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            >  1:1652669/1‑148 (MQ=255)
                                                                       cATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCgcgc                                                                         >  2:1021543/1‑148 (MQ=255)
                                                                         tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAc                                                                       >  2:2190593/1‑148 (MQ=255)
                                                                           aCTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCCGCGGCGGAAAGTCGCGCACCa                                                                     >  1:2571163/1‑148 (MQ=255)
                                                                            cTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAg                                                                    >  1:1776269/1‑148 (MQ=255)
                                                                            cTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAg                                                                    >  2:69029/1‑148 (MQ=255)
                                                                                cACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCATCCGGATCGCCTTCCGGCGGCGGAATGTCGCCCACCAGACga                                                                >  2:3318879/1‑147 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCGTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                              >  1:1965800/1‑148 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                              <  1:2541037/148‑1 (MQ=255)
                                                                                  cTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTc                                                              <  1:2542405/148‑1 (MQ=255)
                                                                                  cTTTGTCGACCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCCGACGTTc                                                              >  2:2127336/1‑148 (MQ=255)
                                                                                   tttGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCg                                                             <  1:732068/148‑1 (MQ=255)
                                                                                    ttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga                                                            <  2:1178954/148‑1 (MQ=255)
                                                                                     tGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgag                                                           <  1:2032324/148‑1 (MQ=255)
                                                                                     tGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgag                                                           <  1:3401051/148‑1 (MQ=255)
                                                                                      gTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgaga                                                          <  1:1129191/148‑1 (MQ=255)
                                                                                           ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCAGACGTTCGAGAACGTc                                                     >  2:1320343/1‑148 (MQ=255)
                                                                                                   aGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCacac                                             <  1:2839540/148‑1 (MQ=255)
                                                                                                   aGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCacac                                             <  1:2840060/148‑1 (MQ=255)
                                                                                                   aGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCacac                                             <  1:3242769/148‑1 (MQ=255)
                                                                                                     ggTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCa                                           <  2:3565001/148‑1 (MQ=255)
                                                                                                     ggTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCa                                           <  2:1501902/148‑1 (MQ=255)
                                                                                                              tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  >  1:1968921/1‑148 (MQ=255)
                                                                                                              tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  >  1:3560803/1‑148 (MQ=255)
                                                                                                              tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  1:3033353/148‑1 (MQ=255)
                                                                                                              tttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  1:3033396/148‑1 (MQ=255)
                                                                                                                   tACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGc                             >  1:3384650/1‑148 (MQ=255)
                                                                                                                    aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                            <  1:1636716/148‑1 (MQ=255)
                                                                                                                    aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                            >  2:1384996/1‑148 (MQ=255)
                                                                                                                    aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                            >  1:2426902/1‑148 (MQ=255)
                                                                                                                    aCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt                            >  2:123585/1‑148 (MQ=255)
                                                                                                                     cGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTg                           >  1:321915/1‑148 (MQ=255)
                                                                                                                        aTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAc                        >  1:2016742/1‑148 (MQ=255)
                                                                                                                        aTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAc                        >  1:2617708/1‑148 (MQ=255)
                                                                                                                        aTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAc                        >  1:895391/1‑148 (MQ=255)
                                                                                                                        aTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAc                        >  1:1188448/1‑148 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                      >  2:449097/1‑148 (MQ=255)
                                                                                                                            gTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcg                    >  2:1997147/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   >  2:749265/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   <  1:2021765/148‑1 (MQ=255)
                                                                                                                              gAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa                  <  1:705147/148‑1 (MQ=255)
                                                                                                                              gAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa                  <  1:3484240/148‑1 (MQ=255)
                                                                                                                              gAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa                  >  1:2565364/1‑148 (MQ=255)
                                                                                                                               aaaCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGAGGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc                 <  2:1361995/148‑1 (MQ=255)
                                                                                                                                  caacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGAACAGCGCACcgc              >  1:1523791/1‑148 (MQ=255)
                                                                                                                                     cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc           >  1:1247578/1‑148 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  2:1676361/148‑1 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  1:420837/148‑1 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  1:2214914/148‑1 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  1:1021543/148‑1 (MQ=255)
                                                                                                                                          aGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTgg      >  2:1364180/1‑148 (MQ=255)
                                                                                                                                           ggTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc     >  2:2787048/1‑148 (MQ=255)
                                                                                                                                           ggTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc     >  1:3162320/1‑148 (MQ=255)
                                                                                                                                            gtagtaGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCg    <  2:140468/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGC  >  CP009273/2699731‑2700029
                                                                                                                                                     |                                                                                                                                                     
TTTCATTTCAGTGCGTTCAAACTGTTTCGGCGTGATGATGCCAAGTCGGTGGGCTTTATAGGTCTGCCCGGTACTCATGAATTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATCCGGATAAGTGAAACAACGCCCAGGTAGTA                                                                                                                                                       <  SRR19973066.2614945/150‑1 (MQ=60)
  TCAGTTCTGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTAACTTTGTCGCCCTTCCGCTGGGTGCGGTTTTTAATACGGATATGTGAAACAACGCCCAGGTAGTAGT                                                                                                                                                     <  SRR19973066.125388/150‑1 (MQ=60)
  TCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGT                                                                                                                                                     >  SRR19973066.2810660/1‑150 (MQ=60)
  TCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGT                                                                                                                                                     >  SRR19973066.1429777/1‑150 (MQ=60)
   CAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTC                                                                                                                                                    <  SRR19973066.240347/150‑1 (MQ=60)
   CAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTC                                                                                                                                                    <  SRR19973066.240300/150‑1 (MQ=60)
   CAGTTCAGTGCGGTCAACCTGTTTCGGCGTCAATATGCCCAGACGGTCGGCGTTATAGGTCTGACCCGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGATTAATAAGCATAAGTGAAACAAAGCCCAGGTACTAGTC                                                                                                                                                    >  SRR19973066.1044468/1‑150 (MQ=60)
         AGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTGATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGAGAAGTGAAACAACACCCACGTATTAGTCGAACCA                                                                                                                                              >  SRR19973066.2434319/1‑150 (MQ=60)
          GGGCGGTCAACCTGTTTCGGGGTGAAGATGCCCAGACGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCAT                                                                                                                                             <  SRR19973066.1680067/150‑1 (MQ=60)
            GCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATCCGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGA                                                                                                                                           <  SRR19973066.1794758/150‑1 (MQ=60)
             CGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                          <  SRR19973066.2575497/150‑1 (MQ=60)
             CGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAG                                                                                                                                          <  SRR19973066.2575513/150‑1 (MQ=60)
              GGTCAACCTGTTTGGGCGTGAAGAGGCCCAGACGGGCGGGTTTATAGGTCGGCCCGGTACTCATGACTTCCACTTTGGCGCCCTTACGCAGGGTGCGGTTTTTAATACGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                         <  SRR19973066.450068/150‑1 (MQ=60)
              GGTCAACCTGTTTCGGCGTTAAGATGCCCAGACGGTCGGCGTTATTGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATATGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGT                                                                                                                                         <  SRR19973066.450108/150‑1 (MQ=60)
               GTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGGGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTC                                                                                                                                        <  SRR19973066.2007808/150‑1 (MQ=60)
               GTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCGGCCCGGTACTAATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTC                                                                                                                                        <  SRR19973066.2564346/150‑1 (MQ=60)
               GTCAACCTGTTTCGGCGTGAAGAGGCCCAGCCGGTCGGCGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTC                                                                                                                                        <  SRR19973066.1981554/150‑1 (MQ=60)
                 CAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAACCCCCCGGTAGTAGTCGAAACAAGAGTCGC                                                                                                                                      >  SRR19973066.618646/1‑150 (MQ=60)
                 CAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAAAGCCCAGGTAGTAGTCGAAACATGAGTCGA                                                                                                                                      >  SRR19973066.2441790/1‑150 (MQ=60)
                      TGTTTCGGCGTTAAGATGTCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                                 <  SRR19973066.2218409/150‑1 (MQ=60)
                      TGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                                 >  SRR19973066.2600948/1‑150 (MQ=60)
                      TGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATT                                                                                                                                 >  SRR19973066.2600970/1‑150 (MQ=60)
                              CTTGAAGATGCCCAGACGGTGGGGTTTTTTGGTCTGCCCGGTACACATGACTTTCACTTTGTCGCCCTTCCGCAGGGTGCCTTTTTTAATACGGATAAGTGAACCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTG                                                                                                                         <  SRR19973066.37459/150‑1 (MQ=60)
                               GTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATAAGGATAAGTGAAACAACCCCCAGGTAGTAGTCGAACCATGAGTCGATAAATATTGCCTGC                                                                                                                        >  SRR19973066.2134125/1‑150 (MQ=60)
                               GTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGCTAACTGAAACAACCCCCAGGGAGTAGTCGAACCATGAGTCGAgaagttttggctgc                                                                                                                        >  SRR19973066.2134346/1‑136 (MQ=60)
                               GTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGGAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGC                                                                                                                        >  SRR19973066.3224544/1‑150 (MQ=60)
                               GTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATAAGGATAAGTGTAACAACGCCCAGGTAGTAGTCGAACCCTGAGTCGCTAATTAGTGCCAGC                                                                                                                        >  SRR19973066.2134254/1‑150 (MQ=60)
                                 GAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAA                                                                                                                      <  SRR19973066.1803573/150‑1 (MQ=60)
                                     TTGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTAATGATTTTCTCTTTGTCGCCCTTCCGCAGGGTGCCTTTTTTAATACGTATATGTTAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGG                                                                                                                  <  SRR19973066.81940/150‑1 (MQ=60)
                                     ATGCCCAGCCGGGCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGG                                                                                                                  <  SRR19973066.82113/150‑1 (MQ=60)
                                       GCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCC                                                                                                                >  SRR19973066.2081668/1‑150 (MQ=60)
                                       GCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCC                                                                                                                >  SRR19973066.2081639/1‑150 (MQ=60)
                                          CAGAGGGGCGGCTTTATCGGTCGGCCCGGTACTCATGACTTTCACTTTGGCGCCCTTCCGCGGGGTGCCGTTTTTAATCCGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTC                                                                                                             <  SRR19973066.926746/150‑1 (MQ=60)
                                           AGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATAAGGATAAGTTAAAAAAACCCCAGGTAGTAGTCGAAACAAGAGTCGATAATTAGTTCCTGCAACGCGCCTTCC                                                                                                            >  SRR19973066.681341/1‑150 (MQ=60)
                                           AGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTAGAACCATTAGTAGATAATTAtttgcagcaaccggccgtcc                                                                                                            >  SRR19973066.681315/1‑130 (MQ=60)
                                              CGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGA                                                                                                         <  SRR19973066.2984811/150‑1 (MQ=60)
                                                GTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATC                                                                                                       <  SRR19973066.277179/150‑1 (MQ=60)
                                                GTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATC                                                                                                       <  SRR19973066.276685/150‑1 (MQ=60)
                                                     CGTTATAGGTCGGCCCGGTACTCATGACTTTCACTTGGTCGCCCTTACGCTGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTT                                                                                                  <  SRR19973066.1847562/150‑1 (MQ=60)
                                                      GTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCCGGAGTCGATAATTATTGCCTGCAACCGGCCTTCCGGATCGCCTTC                                                                                                 >  SRR19973066.415565/1‑150 (MQ=60)
                                                       ttttcGGTCTGCCCGGTACTCATGACTTACACTTGGTCGCCCTTACGCTGGGTGCGGTTTTTAATACGTATAAGTCAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCC                                                                                                <  SRR19973066.622797/145‑1 (MQ=60)
                                                        TATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCG                                                                                               <  SRR19973066.2465683/150‑1 (MQ=60)
                                                           AGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCG                                                                                            <  SRR19973066.2869990/150‑1 (MQ=60)
                                                             GTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATAAGGATAAGTGAAAAAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTttttgctgcaaacggccgtacggatcgccgtccggcggc                                                                                          >  SRR19973066.316550/1‑111 (MQ=60)
                                                                      GTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACCGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCG                                                                                 >  SRR19973066.1664701/1‑150 (MQ=60)
                                                                             TGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCCGCGGCGGAAAGTCGCGCACCA                                                                          >  SRR19973066.2588610/1‑150 (MQ=60)
                                                                              GACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAG                                                                         >  SRR19973066.1788973/1‑150 (MQ=60)
                                                                                    CACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCGTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTC                                                                   >  SRR19973066.1979689/1‑150 (MQ=60)
                                                                                    CACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTC                                                                   <  SRR19973066.937586/150‑1 (MQ=60)
                                                                                     ACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCG                                                                  <  SRR19973066.2697422/150‑1 (MQ=60)
                                                                                      CTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGA                                                                 <  SRR19973066.2558304/150‑1 (MQ=60)
                                                                                      CTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGA                                                                 <  SRR19973066.2559680/150‑1 (MQ=60)
                                                                                       TTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAG                                                                <  SRR19973066.737500/150‑1 (MQ=60)
                                                                                       TTGGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAG                                                                <  SRR19973066.737511/150‑1 (MQ=60)
                                                                                         TGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAA                                                              <  SRR19973066.2046510/150‑1 (MQ=60)
                                                                                         TGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAA                                                              <  SRR19973066.3423759/150‑1 (MQ=60)
                                                                                          GTCGCCCTTACGCAGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAAC                                                             <  SRR19973066.1137290/150‑1 (MQ=60)
                                                                                          GTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAAC                                                             <  SRR19973066.1137299/150‑1 (MQ=60)
                                                                                                CTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG                                                       <  SRR19973066.32650/150‑1 (MQ=60)
                                                                                                       AGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCA                                                <  SRR19973066.3264159/150‑1 (MQ=60)
                                                                                                       AGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCA                                                <  SRR19973066.2859152/150‑1 (MQ=60)
                                                                                                       AGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCA                                                <  SRR19973066.2858978/150‑1 (MQ=60)
                                                                                                       AGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCA                                                <  SRR19973066.2858629/150‑1 (MQ=60)
                                                                                                               GTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGT                                        >  SRR19973066.1178351/1‑150 (MQ=60)
                                                                                                                TTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTT                                       >  SRR19973066.3584417/1‑150 (MQ=60)
                                                                                                                TTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTT                                       >  SRR19973066.1982829/1‑150 (MQ=60)
                                                                                                                  TTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTT                                     <  SRR19973066.3053665/150‑1 (MQ=60)
                                                                                                                  TTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTT                                     <  SRR19973066.3053619/150‑1 (MQ=60)
                                                                                                                    TAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCG                                   <  SRR19973066.3020183/150‑1 (MQ=60)
                                                                                                                     AATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGC                                  >  SRR19973066.3407365/1‑150 (MQ=60)
                                                                                                                     AATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGC                                  >  SRR19973066.3407234/1‑150 (MQ=60)
                                                                                                                      ATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCT                                 >  SRR19973066.2443392/1‑150 (MQ=60)
                                                                                                                       TACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTG                                >  SRR19973066.324339/1‑150 (MQ=60)
                                                                                                                        ACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGA                               <  SRR19973066.1648612/150‑1 (MQ=60)
                                                                                                                          GGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAC                             >  SRR19973066.901724/1‑150 (MQ=60)
                                                                                                                          GGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAC                             >  SRR19973066.2030863/1‑150 (MQ=60)
                                                                                                                          GGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAC                             >  SRR19973066.2635384/1‑150 (MQ=60)
                                                                                                                          GGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAAC                             >  SRR19973066.1196784/1‑150 (MQ=60)
                                                                                                                                GTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCA                       >  SRR19973066.261225/1‑150 (MQ=60)
                                                                                                                                GTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCA                       >  SRR19973066.2582774/1‑150 (MQ=60)
                                                                                                                                 TGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAC                      <  SRR19973066.2035907/150‑1 (MQ=60)
                                                                                                                                 TGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAC                      <  SRR19973066.2035905/150‑1 (MQ=60)
                                                                                                                                 TGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAC                      <  SRR19973066.1905798/150‑1 (MQ=60)
                                                                                                                                  TAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACC                     <  SRR19973066.710357/150‑1 (MQ=60)
                                                                                                                                  GAAACAACTCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACC                     <  SRR19973066.2921277/150‑1 (MQ=60)
                                                                                                                                  GAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACC                     <  SRR19973066.3507485/150‑1 (MQ=60)
                                                                                                                                    AACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGC                   >  SRR19973066.1534261/1‑150 (MQ=60)
                                                                                                                                    AACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGAACAGCGCACCGC                   >  SRR19973066.1534560/1‑150 (MQ=60)
                                                                                                                                       AACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTC                >  SRR19973066.1256534/1‑150 (MQ=60)
                                                                                                                                           CCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTG            <  SRR19973066.2011193/150‑1 (MQ=60)
                                                                                                                                            CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCCACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG           >  SRR19973066.1302852/1‑150 (MQ=60)
                                                                                                                                            CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG           <  SRR19973066.1029060/150‑1 (MQ=60)
                                                                                                                                            CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG           <  SRR19973066.424111/150‑1 (MQ=60)
                                                                                                                                            CCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGG           <  SRR19973066.2230251/150‑1 (MQ=60)
                                                                                                                                             CAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGC          >  SRR19973066.442819/1‑150 (MQ=60)
                                                                                                                                             CAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGC          >  SRR19973066.1654588/1‑150 (MQ=60)
                                                                                                                                             CAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGC          >  SRR19973066.3183384/1‑150 (MQ=60)
                                                                                                                                               GGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGT        >  SRR19973066.493073/1‑150 (MQ=60)
                                                                                                                                                GTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC       >  SRR19973066.3424954/1‑150 (MQ=60)
                                                                                                                                                 TAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCCACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCG      >  SRR19973066.3401141/1‑150 (MQ=60)
                                                                                                                                                   GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT    <  SRR19973066.1395003/150‑1 (MQ=60)
                                                                                                                                                   GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT    <  SRR19973066.93163/150‑1 (MQ=60)
                                                                                                                                                   GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT    <  SRR19973066.1394061/150‑1 (MQ=60)
                                                                                                                                                   GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT    <  SRR19973066.125675/150‑1 (MQ=60)
                                                                                                                                                   GTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAT    <  SRR19973066.124824/150‑1 (MQ=60)
                                                                                                                                                    TAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG   <  SRR19973066.1555625/150‑1 (MQ=60)
                                                                                                                                                    TAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG   >  SRR19973066.1755990/1‑150 (MQ=60)
                                                                                                                                                    TAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATG   >  SRR19973066.1756471/1‑150 (MQ=60)
                                                                                                                                                     AGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGC  >  SRR19973066.1535137/1‑150 (MQ=60)
                                                                                                                                                     AGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGC  >  SRR19973066.1535847/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGC  >  CP009273/2699731‑2700029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑