Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,088,850 C→A 94.7% R93S (CGT→AGT)  rhaR → transcriptional activator of rhaSR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,088,8500CA94.7% 529.7 / 19.2 181R93S (CGT→AGT) rhaRtranscriptional activator of rhaSR
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base A (106/65);  total (111/70)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994
                                                                                                                                                 |                                                                                                                                                
 tGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTc                                                                                                                                               >  1:2256104/1‑148 (MQ=255)
  ggCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCt                                                                                                                                              <  1:230911/148‑1 (MQ=255)
    cgcgGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGa                                                                                                                                            >  1:647980/1‑148 (MQ=255)
      cgGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAg                                                                                                                                          >  2:2372012/1‑148 (MQ=255)
       ggTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGc                                                                                                                                         >  1:275660/1‑148 (MQ=255)
        gTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCt                                                                                                                                        <  2:1692914/148‑1 (MQ=255)
         tAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTg                                                                                                                                       >  1:1183195/1‑148 (MQ=255)
           aTGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGaa                                                                                                                                     >  1:3114181/1‑148 (MQ=255)
             ggCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATc                                                                                                                                   <  2:2295423/148‑1 (MQ=255)
                 tGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGa                                                                                                                               >  1:2451573/1‑148 (MQ=255)
                 tGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGa                                                                                                                               <  1:2381639/148‑1 (MQ=255)
                   cATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACt                                                                                                                             >  1:736227/1‑148 (MQ=255)
                       tACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCa                                                                                                                         <  2:2855541/148‑1 (MQ=255)
                        aCTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAg                                                                                                                        >  1:391305/1‑148 (MQ=255)
                        aCTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAg                                                                                                                        >  1:391244/1‑148 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  1:183686/1‑148 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  1:2410868/1‑148 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  1:2411101/1‑148 (MQ=255)
                                aTCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAt                                                                                                                >  1:2501165/1‑148 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         <  1:1381993/148‑1 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         >  1:849965/1‑148 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         <  1:2372012/148‑1 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         >  2:605827/1‑148 (MQ=255)
                                        tATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGAt                                                                                                        >  2:2197885/1‑148 (MQ=255)
                                        tATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTAGTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGAt                                                                                                        <  1:3165444/148‑1 (MQ=255)
                                          tCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGAttt                                                                                                      >  2:844618/1‑148 (MQ=255)
                                              aTTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACg                                                                                                  <  2:335158/148‑1 (MQ=255)
                                               ttACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGc                                                                                                 <  1:1986710/148‑1 (MQ=255)
                                                   ccGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAgc                                                                                             >  1:86988/1‑148 (MQ=255)
                                                     gTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAgcgc                                                                                           <  2:539910/148‑1 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATTAACACTACTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         <  1:2701998/148‑1 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         <  1:2702033/148‑1 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  1:1143525/1‑148 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  2:697881/1‑148 (MQ=255)
                                                        gCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAgg                                                                                        <  2:962699/148‑1 (MQ=255)
                                                         cGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAggg                                                                                       >  2:1201547/1‑148 (MQ=255)
                                                         cGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAggg                                                                                       >  2:2388091/1‑148 (MQ=255)
                                                           aTCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCa                                                                                     >  2:2862005/1‑148 (MQ=255)
                                                           aTCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCa                                                                                     >  1:2470795/1‑148 (MQ=255)
                                                           aTCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCa                                                                                     >  2:3124426/1‑148 (MQ=255)
                                                             ctctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAc                                                                                   >  1:1845881/1‑148 (MQ=255)
                                                             ctctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAc                                                                                   <  2:1542704/148‑1 (MQ=255)
                                                             ctctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAc                                                                                   >  2:2256268/1‑148 (MQ=255)
                                                             ctctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAc                                                                                   >  2:594107/1‑148 (MQ=255)
                                                              tctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAcc                                                                                  >  2:1401192/1‑148 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               >  1:2548692/1‑148 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               >  2:3215117/1‑148 (MQ=255)
                                                                   tACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACt                                                                             <  2:2138828/148‑1 (MQ=255)
                                                                   tACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACt                                                                             <  1:2460739/148‑1 (MQ=255)
                                                                    aCATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTg                                                                            >  2:1928586/1‑148 (MQ=255)
                                                                      aTTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgc                                                                          >  1:1694687/1‑148 (MQ=255)
                                                                       ttCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgcg                                                                         <  2:925706/148‑1 (MQ=255)
                                                                        tCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgcgc                                                                        <  1:231155/148‑1 (MQ=255)
                                                                         cATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCt                                                                       >  1:2291994/1‑148 (MQ=255)
                                                                            gCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAg                                                                    <  2:452275/148‑1 (MQ=255)
                                                                             cTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAgg                                                                   <  1:2849585/148‑1 (MQ=255)
                                                                              tGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGt                                                                  <  2:275660/148‑1 (MQ=255)
                                                                                aCGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAg                                                                >  2:1387793/1‑148 (MQ=255)
                                                                                aCGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAg                                                                <  1:1953476/148‑1 (MQ=255)
                                                                                    tAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCCGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTCGGTAGCATg                                                            >  2:2884870/1‑148 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          <  2:1028510/148‑1 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          >  1:64096/1‑148 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          <  1:2332789/148‑1 (MQ=255)
                                                                                       acacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATgggg                                                         <  2:1972782/148‑1 (MQ=255)
                                                                                         acTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAt                                                       <  1:269563/148‑1 (MQ=255)
                                                                                         acTCCGACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAt                                                       <  1:2834748/148‑1 (MQ=255)
                                                                                              tACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGgcgc                                                  >  2:2758103/1‑148 (MQ=255)
                                                                                              tACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGgcgc                                                  >  2:21993/1‑148 (MQ=255)
                                                                                                      cGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCgg                                          <  1:593563/148‑1 (MQ=255)
                                                                                                      cGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCgg                                          >  2:3198229/1‑148 (MQ=255)
                                                                                                      cGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCgg                                          >  2:1579340/1‑148 (MQ=255)
                                                                                                      cGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCgg                                          >  2:1579297/1‑148 (MQ=255)
                                                                                                        ttAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATATTGACTGGCAGCGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAAGGCGCAGGCGCGGCa                                        >  1:2065246/1‑148 (MQ=255)
                                                                                                          aaCGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAgg                                      >  1:296696/1‑148 (MQ=255)
                                                                                                           aCGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGt                                     <  2:2920026/148‑1 (MQ=255)
                                                                                                              aTCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTAt                                  <  1:1401192/148‑1 (MQ=255)
                                                                                                               tCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATc                                 >  2:1175034/1‑148 (MQ=255)
                                                                                                                  ggTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGt                              <  2:2561499/148‑1 (MQ=255)
                                                                                                                  ggTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGt                              >  1:2236469/1‑148 (MQ=255)
                                                                                                                   gTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTc                             <  2:1078629/148‑1 (MQ=255)
                                                                                                                    ttttGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCa                            >  2:1942731/1‑148 (MQ=255)
                                                                                                                      ttGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGc                          <  2:832779/148‑1 (MQ=255)
                                                                                                                       tGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTTGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCt                         <  1:3075613/148‑1 (MQ=255)
                                                                                                                       tGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCt                         <  2:849965/148‑1 (MQ=255)
                                                                                                                         cAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTg                       >  2:1363134/1‑148 (MQ=255)
                                                                                                                          aGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGa                      <  2:1869410/148‑1 (MQ=255)
                                                                                                                           gAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAg                     >  2:1243931/1‑148 (MQ=255)
                                                                                                                           gAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAg                     <  1:756796/148‑1 (MQ=255)
                                                                                                                            aaTATTATTTATTGCCCGGAGAGTCTGAAGCCGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGc                    >  2:1818772/1‑148 (MQ=255)
                                                                                                                               attattTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATg                 >  2:638126/1‑148 (MQ=255)
                                                                                                                               attattTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATg                 <  2:2548692/148‑1 (MQ=255)
                                                                                                                                 tatttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaa               >  1:2992584/1‑148 (MQ=255)
                                                                                                                                   tttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAag             <  2:3024354/148‑1 (MQ=255)
                                                                                                                                     tattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagta           >  2:2773669/1‑148 (MQ=255)
                                                                                                                                      attGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtag          >  1:662732/1‑148 (MQ=255)
                                                                                                                                       ttGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtagt         <  2:736227/148‑1 (MQ=255)
                                                                                                                                       ttGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtagt         >  1:274957/1‑148 (MQ=255)
                                                                                                                                          cccGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAg      <  2:647980/148‑1 (MQ=255)
                                                                                                                                            cGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCa    >  1:2338549/1‑148 (MQ=255)
                                                                                                                                              gagaGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCAtg  <  2:875226/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT  >  CP009273/4088704‑4088995
                                                                                                                                                  |                                                                                                                                                 
TCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTC                                                                                                                                                >  SRR19973070.2271063/1‑150 (MQ=60)
 CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCT                                                                                                                                               <  SRR19973070.2471874/150‑1 (MQ=60)
   GGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGA                                                                                                                                             >  SRR19973070.652604/1‑150 (MQ=60)
   GGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGA                                                                                                                                             <  SRR19973070.232542/150‑1 (MQ=60)
      GCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGC                                                                                                                                          >  SRR19973070.277452/1‑150 (MQ=60)
        GGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTG                                                                                                                                        >  SRR19973070.1191510/1‑150 (MQ=60)
          TAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAA                                                                                                                                      >  SRR19973070.3134279/1‑150 (MQ=60)
           AATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTTAAGCTGAAT                                                                                                                                     <  SRR19973070.2559583/150‑1 (MQ=60)
           AATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAT                                                                                                                                     >  SRR19973070.1986117/1‑150 (MQ=60)
              GGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTT                                                                                                                                  <  SRR19973070.2570099/150‑1 (MQ=60)
                CCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGA                                                                                                                                >  SRR19973070.2467611/1‑150 (MQ=60)
                  TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                              <  SRR19973070.2397302/150‑1 (MQ=60)
                  TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                              >  SRR19973070.740979/1‑150 (MQ=60)
                  TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                              <  SRR19973070.2397376/150‑1 (MQ=60)
                  TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                              >  SRR19973070.741438/1‑150 (MQ=60)
                       GTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAG                                                                                                                         >  SRR19973070.394179/1‑150 (MQ=60)
                       GTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAG                                                                                                                         >  SRR19973070.394118/1‑150 (MQ=60)
                       GTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAG                                                                                                                         >  SRR19973070.3303624/1‑150 (MQ=60)
                         ACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGG                                                                                                                       <  SRR19973070.3109954/150‑1 (MQ=60)
                             AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                   >  SRR19973070.844844/1‑150 (MQ=60)
                             AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                   >  SRR19973070.2426985/1‑150 (MQ=60)
                             AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                   >  SRR19973070.2426892/1‑150 (MQ=60)
                             AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                   >  SRR19973070.2426752/1‑150 (MQ=60)
                             AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                   >  SRR19973070.185122/1‑150 (MQ=60)
                               CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                 >  SRR19973070.2517400/1‑150 (MQ=60)
                                 ATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTC                                                                                                               >  SRR19973070.645973/1‑150 (MQ=60)
                                      CCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGA                                                                                                          >  SRR19973070.855793/1‑150 (MQ=60)
                                      CCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATACCGGGA                                                                                                          >  SRR19973070.855918/1‑150 (MQ=60)
                                        TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                        <  SRR19973070.1391811/150‑1 (MQ=60)
                                        TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                        <  SRR19973070.1391354/150‑1 (MQ=60)
                                        TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                        <  SRR19973070.2387701/150‑1 (MQ=60)
                                         TATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTT                                                                                                       >  SRR19973070.2938881/1‑150 (MQ=60)
                                         TATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTAGTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTT                                                                                                       <  SRR19973070.3185924/150‑1 (MQ=60)
                                              CATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGC                                                                                                  >  SRR19973070.1944884/1‑150 (MQ=60)
                                              CATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGC                                                                                                  >  SRR19973070.2760460/1‑150 (MQ=60)
                                                TTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCA                                                                                                <  SRR19973070.2000166/150‑1 (MQ=60)
                                                  ACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGC                                                                                              >  SRR19973070.87868/1‑150 (MQ=60)
                                                      GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                          >  SRR19973070.1151462/1‑150 (MQ=60)
                                                        GGCGATCTCTTTTACATTCATGCTGACGATTAACACTACTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGG                                                                                        <  SRR19973070.2719475/150‑1 (MQ=60)
                                                        GGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGG                                                                                        <  SRR19973070.2719510/150‑1 (MQ=60)
                                                          CGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCA                                                                                      >  SRR19973070.2486900/1‑150 (MQ=60)
                                                            ATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAC                                                                                    >  SRR19973070.1858545/1‑150 (MQ=60)
                                                                CTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACA                                                                                >  SRR19973070.2565359/1‑150 (MQ=60)
                                                                 TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                               >  SRR19973070.1786936/1‑150 (MQ=60)
                                                                    TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                            <  SRR19973070.2476807/150‑1 (MQ=60)
                                                                     ACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGC                                                                           >  SRR19973070.1706347/1‑150 (MQ=60)
                                                                     ACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGC                                                                           >  SRR19973070.2814221/1‑150 (MQ=60)
                                                                        TTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCT                                                                        >  SRR19973070.2307237/1‑150 (MQ=60)
                                                                         TCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTT                                                                       <  SRR19973070.232777/150‑1 (MQ=60)
                                                                         TCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTT                                                                       <  SRR19973070.232787/150‑1 (MQ=60)
                                                                          CATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTA                                                                      >  SRR19973070.2455379/1‑150 (MQ=60)
                                                                              CTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGTTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGNA                                                                  <  SRR19973070.2868041/150‑1 (MQ=60)
                                                                              CTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTA                                                                  <  SRR19973070.450165/150‑1 (MQ=60)
                                                                                GACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGC                                                                >  SRR19973070.1345247/1‑150 (MQ=60)
                                                                                 ACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCA                                                               <  SRR19973070.1966619/150‑1 (MQ=60)
                                                                                    ATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGG                                                            >  SRR19973070.2263613/1‑150 (MQ=60)
                                                                                     TNAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGG                                                           >  SRR19973070.64817/1‑150 (MQ=60)
                                                                                     TAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGG                                                           >  SRR19973070.63196/1‑150 (MQ=60)
                                                                                       AACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGA                                                         <  SRR19973070.2348269/150‑1 (MQ=60)
                                                                                       AACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGA                                                         <  SRR19973070.2348281/150‑1 (MQ=60)
                                                                                        ACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAT                                                        >  SRR19973070.2495958/1‑150 (MQ=60)
                                                                                        ACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAT                                                        >  SRR19973070.2321566/1‑150 (MQ=60)
                                                                                          ACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGG                                                      <  SRR19973070.271334/150‑1 (MQ=60)
                                                                                          ACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGG                                                      <  SRR19973070.2853158/150‑1 (MQ=60)
                                                                                          ACTCCGACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGG                                                      <  SRR19973070.2853091/150‑1 (MQ=60)
                                                                                            TCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCG                                                    >  SRR19973070.271850/1‑150 (MQ=60)
                                                                                            TCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCG                                                    >  SRR19973070.2002816/1‑150 (MQ=60)
                                                                                                       CGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATATTGACTGGCAGCGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAAGGCGCAGGCGCGGCA                                         >  SRR19973070.2079174/1‑150 (MQ=60)
                                                                                                       CGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCA                                         <  SRR19973070.597673/150‑1 (MQ=60)
                                                                                                         TTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGG                                       >  SRR19973070.298750/1‑150 (MQ=60)
                                                                                                               ATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCG                                 <  SRR19973070.1410651/150‑1 (MQ=60)
                                                                                                                 CTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGT                               >  SRR19973070.2251207/1‑150 (MQ=60)
                                                                                                                 CTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGT                               >  SRR19973070.2251220/1‑150 (MQ=60)
                                                                                                                        TGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTTGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTG                        <  SRR19973070.3095366/150‑1 (MQ=60)
                                                                                                                        TGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTG                        <  SRR19973070.3094555/150‑1 (MQ=60)
                                                                                                                        TGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTG                        >  SRR19973070.1427556/1‑150 (MQ=60)
                                                                                                                            GAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCA                    <  SRR19973070.762132/150‑1 (MQ=60)
                                                                                                                                ATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAA                >  SRR19973070.3011752/1‑150 (MQ=60)
                                                                                                                                   ATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGT             >  SRR19973070.2426484/1‑150 (MQ=60)
                                                                                                                                     TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG           >  SRR19973070.666899/1‑150 (MQ=60)
                                                                                                                                     TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG           >  SRR19973070.667459/1‑150 (MQ=60)
                                                                                                                                      TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT          >  SRR19973070.276747/1‑150 (MQ=60)
                                                                                                                                      TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT          >  SRR19973070.276770/1‑150 (MQ=60)
                                                                                                                                         TGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAG       >  SRR19973070.3157710/1‑150 (MQ=60)
                                                                                                                                           CCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCA     >  SRR19973070.2353823/1‑150 (MQ=60)
                                                                                                                                           CCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCA     >  SRR19973070.2354069/1‑150 (MQ=60)
                                                                                                                                              GGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT  >  SRR19973070.989354/1‑150 (MQ=60)
                                                                                                                                              GGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT  >  SRR19973070.990539/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                 
TCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT  >  CP009273/4088704‑4088995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑