Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,088,850 C→A 100% R93S (CGT→AGT)  rhaR → transcriptional activator of rhaSR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,088,8500CA98.5% 632.6 / 0.6 196R93S (CGT→AGT) rhaRtranscriptional activator of rhaSR
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (89/104);  total (90/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994
                                                                                                                                                 |                                                                                                                                                
cTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGt                                                                                                                                                <  1:1049806/148‑1 (MQ=255)
cTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGt                                                                                                                                                >  1:1540873/1‑148 (MQ=255)
      cgGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAg                                                                                                                                          <  1:1045369/148‑1 (MQ=255)
       ggTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGc                                                                                                                                         <  1:1014385/148‑1 (MQ=255)
         tAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTg                                                                                                                                       >  1:2475144/1‑148 (MQ=255)
          aaTGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGa                                                                                                                                      >  2:1665819/1‑148 (MQ=255)
            tGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAt                                                                                                                                    >  2:850074/1‑148 (MQ=255)
             ggCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATc                                                                                                                                   <  1:3485759/148‑1 (MQ=255)
               ccTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCtt                                                                                                                                 >  2:1948318/1‑148 (MQ=255)
                 tGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGa                                                                                                                               >  2:803082/1‑148 (MQ=255)
                 tGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGa                                                                                                                               <  1:3338416/148‑1 (MQ=255)
                   cATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACt                                                                                                                             <  2:533550/148‑1 (MQ=255)
                     tGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTgg                                                                                                                           <  2:290716/148‑1 (MQ=255)
                     tGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTgg                                                                                                                           >  2:1590805/1‑148 (MQ=255)
                     tGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTgg                                                                                                                           >  1:2593404/1‑148 (MQ=255)
                      gTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGc                                                                                                                          <  2:2547838/148‑1 (MQ=255)
                        aCTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAg                                                                                                                        <  2:1357350/148‑1 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     <  1:756360/148‑1 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     >  1:2991484/1‑148 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     >  1:2374442/1‑148 (MQ=255)
                            aaCGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATGGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAggggg                                                                                                                    <  2:460211/148‑1 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  <  1:2221741/148‑1 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  2:226037/1‑148 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  <  1:639537/148‑1 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  <  1:2755780/148‑1 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  <  1:2755210/148‑1 (MQ=255)
                               gATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGa                                                                                                                 <  2:2638079/148‑1 (MQ=255)
                               gATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGa                                                                                                                 <  1:3502219/148‑1 (MQ=255)
                                 tCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAtt                                                                                                               <  1:2407457/148‑1 (MQ=255)
                                  cGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTc                                                                                                              <  1:2394589/148‑1 (MQ=255)
                                   gCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTcc                                                                                                             <  1:544379/148‑1 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         >  1:961054/1‑148 (MQ=255)
                                          tCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGAttt                                                                                                      >  2:1893182/1‑148 (MQ=255)
                                           cGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTa                                                                                                     >  2:3549106/1‑148 (MQ=255)
                                             cATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAc                                                                                                   >  1:2826166/1‑148 (MQ=255)
                                               ttACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGc                                                                                                 <  1:2186782/148‑1 (MQ=255)
                                               ttACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGc                                                                                                 <  2:1396115/148‑1 (MQ=255)
                                                tACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGcc                                                                                                >  1:2609293/1‑148 (MQ=255)
                                                tACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGcc                                                                                                >  1:1225074/1‑148 (MQ=255)
                                                    cGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAgcg                                                                                            >  2:1165773/1‑148 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         <  1:3006158/148‑1 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         <  1:435498/148‑1 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  1:3129037/1‑148 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  1:2496611/1‑148 (MQ=255)
                                                         cGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAggg                                                                                       <  2:1470327/148‑1 (MQ=255)
                                                         cGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAggg                                                                                       >  2:1718011/1‑148 (MQ=255)
                                                            tctcTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCaa                                                                                    >  1:1585625/1‑148 (MQ=255)
                                                              tctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAcc                                                                                  <  1:2398186/148‑1 (MQ=255)
                                                               ctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACca                                                                                 <  2:1247389/148‑1 (MQ=255)
                                                               ctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACca                                                                                 <  2:33573/148‑1 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               >  1:755160/1‑148 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               >  2:3202366/1‑148 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               <  2:2129001/148‑1 (MQ=255)
                                                                  ttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCacac                                                                              <  2:2846173/148‑1 (MQ=255)
                                                                        tCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGACCGGAGAGTCTGAAGCTGAATCTTGACTGTCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgcgc                                                                        >  1:991078/1‑148 (MQ=255)
                                                                         cATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCt                                                                       >  2:329230/1‑148 (MQ=255)
                                                                             cTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAgg                                                                   <  1:374323/148‑1 (MQ=255)
                                                                                aCGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAg                                                                >  2:386332/1‑148 (MQ=255)
                                                                                  gATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCa                                                              >  2:2435911/1‑148 (MQ=255)
                                                                                    tAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATg                                                            >  1:1505303/1‑148 (MQ=255)
                                                                                    tAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATg                                                            <  2:1824497/148‑1 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          <  2:83469/148‑1 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          <  2:83799/148‑1 (MQ=255)
                                                                                      aaCACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATggg                                                          >  2:2366003/1‑148 (MQ=255)
                                                                                       acacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATgggg                                                         <  2:3121858/148‑1 (MQ=255)
                                                                                       acacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATgggg                                                         <  2:2733089/148‑1 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        <  1:2928915/148‑1 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        <  2:246654/148‑1 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        >  1:1408456/1‑148 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        <  2:509049/148‑1 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        <  2:2302327/148‑1 (MQ=255)
                                                                                           tCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATgg                                                     >  1:2543312/1‑148 (MQ=255)
                                                                                              tACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATGGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGgcgc                                                  <  1:1527056/148‑1 (MQ=255)
                                                                                               aCGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCa                                                 <  1:270443/148‑1 (MQ=255)
                                                                                                 gCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAgg                                               <  1:2217538/148‑1 (MQ=255)
                                                                                                         tAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAg                                       >  2:1521550/1‑148 (MQ=255)
                                                                                                         tAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAg                                       >  1:203278/1‑148 (MQ=255)
                                                                                                          aaCGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAgg                                      <  1:1718011/148‑1 (MQ=255)
                                                                                                            cGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGtt                                    >  2:2805380/1‑148 (MQ=255)
                                                                                                             gATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTa                                   <  1:2366003/148‑1 (MQ=255)
                                                                                                              aTCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTAt                                  <  2:516799/148‑1 (MQ=255)
                                                                                                              aTCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTAt                                  <  1:1590805/148‑1 (MQ=255)
                                                                                                                 tGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCgg                               <  1:987584/148‑1 (MQ=255)
                                                                                                                  ggTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGt                              <  2:2180416/148‑1 (MQ=255)
                                                                                                                    ttttGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCa                            <  2:2475144/148‑1 (MQ=255)
                                                                                                                         cAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTg                       >  2:665346/1‑148 (MQ=255)
                                                                                                                         cAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTg                       >  2:716619/1‑148 (MQ=255)
                                                                                                                              tattatTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAt                  >  1:1601197/1‑148 (MQ=255)
                                                                                                                               attattTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATg                 >  1:2135585/1‑148 (MQ=255)
                                                                                                                               attattTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATg                 <  1:2758913/148‑1 (MQ=255)
                                                                                                                                 tatttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaa               <  1:788607/148‑1 (MQ=255)
                                                                                                                                  atttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaaa              <  2:1918176/148‑1 (MQ=255)
                                                                                                                                  atttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaaa              >  1:2208947/1‑148 (MQ=255)
                                                                                                                                    ttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagt            <  1:3073731/148‑1 (MQ=255)
                                                                                                                                    ttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCGTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagt            >  2:2759850/1‑148 (MQ=255)
                                                                                                                                       ttGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtagt         >  1:3475774/1‑148 (MQ=255)
                                                                                                                                          cccGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAg      <  1:1959770/148‑1 (MQ=255)
                                                                                                                                           ccGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGc     <  1:2435911/148‑1 (MQ=255)
                                                                                                                                            cGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCa    <  1:1425905/148‑1 (MQ=255)
                                                                                                                                            cGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCa    <  2:961054/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  >  CP009273/4088703‑4088999
                                                                                                                                                   |                                                                                                                                                     
GTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGT                                                                                                                                                     >  SRR19973066.1551886/1‑150 (MQ=60)
  CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCT                                                                                                                                                   <  SRR19973066.1057175/150‑1 (MQ=60)
  CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCT                                                                                                                                                   <  SRR19973066.1057525/150‑1 (MQ=60)
   TGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTG                                                                                                                                                  <  SRR19973066.3081054/150‑1 (MQ=60)
        CGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCT                                                                                                                                             <  SRR19973066.1053051/150‑1 (MQ=60)
         GGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTG                                                                                                                                            <  SRR19973066.1021854/150‑1 (MQ=60)
         GGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTG                                                                                                                                            >  SRR19973066.2492026/1‑150 (MQ=60)
         GGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTG                                                                                                                                            >  SRR19973066.2491973/1‑150 (MQ=60)
             ATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATC                                                                                                                                        <  SRR19973066.1979560/150‑1 (MQ=60)
             ATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATC                                                                                                                                        >  SRR19973066.1565507/1‑150 (MQ=60)
               GGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTT                                                                                                                                      <  SRR19973066.3509012/150‑1 (MQ=60)
                  CTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGAC                                                                                                                                   <  SRR19973066.2426042/150‑1 (MQ=60)
                   TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                                  <  SRR19973066.3360638/150‑1 (MQ=60)
                     CATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGG                                                                                                                                >  SRR19973066.2610970/1‑150 (MQ=60)
                     CATGTACTCAACGATCGCCCTTAGCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGG                                                                                                                                <  SRR19973066.981479/150‑1 (MQ=60)
                           CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          >  SRR19973066.3011293/1‑150 (MQ=60)
                           CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          >  SRR19973066.2390481/1‑150 (MQ=60)
                             CAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGC                                                                                                                        <  SRR19973066.761901/150‑1 (MQ=60)
                             CAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGC                                                                                                                        <  SRR19973066.761963/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.2774323/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.2774342/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.2773762/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.2236381/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.2237106/150‑1 (MQ=60)
                                CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     <  SRR19973066.644154/150‑1 (MQ=60)
                                 GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    <  SRR19973066.3525570/150‑1 (MQ=60)
                                   TCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCC                                                                                                                  <  SRR19973066.2423747/150‑1 (MQ=60)
                                   TCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCC                                                                                                                  <  SRR19973066.2423701/150‑1 (MQ=60)
                                    CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 <  SRR19973066.2410713/150‑1 (MQ=60)
                                     GCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGG                                                                                                                <  SRR19973066.548311/150‑1 (MQ=60)
                                     GCCCTTATCGAATTACCCGTGGAGATCTCTTTTACATTCATGCTGACGATTAACAATACTTCGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAATCTGATTCTTGACTGGCAGGGGGCGATTCCGG                                                                                                                <  SRR19973066.2351873/150‑1 (MQ=60)
                                       CCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGA                                                                                                              >  SRR19973066.968104/1‑150 (MQ=60)
                                         TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                            <  SRR19973066.372654/150‑1 (MQ=60)
                                             CGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAC                                                                                                        >  SRR19973066.2843968/1‑150 (MQ=60)
                                             CGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAC                                                                                                        >  SRR19973066.2195550/1‑150 (MQ=60)
                                             CGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAC                                                                                                        >  SRR19973066.2845152/1‑150 (MQ=60)
                                             CGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAC                                                                                                        <  SRR19973066.420203/150‑1 (MQ=60)
                                               CATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGC                                                                                                      >  SRR19973066.3515146/1‑150 (MQ=60)
                                                ATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCC                                                                                                     >  SRR19973066.2626499/1‑150 (MQ=60)
                                                ATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCC                                                                                                     >  SRR19973066.2626934/1‑150 (MQ=60)
                                                ATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCC                                                                                                     >  SRR19973066.1233746/1‑150 (MQ=60)
                                                 TTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCA                                                                                                    <  SRR19973066.2201955/150‑1 (MQ=60)
                                                 TTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCA                                                                                                    <  SRR19973066.2202136/150‑1 (MQ=60)
                                                       GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                              >  SRR19973066.2513627/1‑150 (MQ=60)
                                                       GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                              >  SRR19973066.3149925/1‑150 (MQ=60)
                                                       GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                              >  SRR19973066.2513273/1‑150 (MQ=60)
                                                         GGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGG                                                                                            >  SRR19973066.2994737/1‑150 (MQ=60)
                                                         GGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGG                                                                                            <  SRR19973066.438872/150‑1 (MQ=60)
                                                         GGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGG                                                                                            <  SRR19973066.3026152/150‑1 (MQ=60)
                                                           CGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCA                                                                                          >  SRR19973066.1736543/1‑150 (MQ=60)
                                                            GATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAA                                                                                         >  SRR19973066.2387774/1‑150 (MQ=60)
                                                            GATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAA                                                                                         >  SRR19973066.1597094/1‑150 (MQ=60)
                                                            GATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAA                                                                                         >  SRR19973066.1597205/1‑150 (MQ=60)
                                                             ATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAC                                                                                        >  SRR19973066.781690/1‑150 (MQ=60)
                                                                TCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCAC                                                                                     <  SRR19973066.2414363/150‑1 (MQ=60)
                                                                 CTTTTACATTCTTGCTGACGATAAACACTCCTACGCTTCCTTTAACGTTCTTGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGTAGCTGAATCTTGACTGGCAGGGGGCGATTCGGGTATTTAACGCCAGCGCAGGGCAACCACA                                                                                    <  SRR19973066.1551865/150‑1 (MQ=60)
                                                                 CTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACA                                                                                    >  SRR19973066.760757/1‑150 (MQ=60)
                                                                   TTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACAGGCACCGGGCCCTTCCGGGATTTAACGCCAGCGCAGGGCAACCACACT                                                                                  <  SRR19973066.3420822/150‑1 (MQ=60)
                                                                     TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                                >  SRR19973066.100060/1‑150 (MQ=60)
                                                                        ATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGACCGGAGAGTCTGAAGCTGAATCTTGACTGTCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGC                                                                             >  SRR19973066.998386/1‑150 (MQ=60)
                                                                         TTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCT                                                                            <  SRR19973066.2700967/150‑1 (MQ=60)
                                                                          TCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTT                                                                           >  SRR19973066.677435/1‑150 (MQ=60)
                                                                               CTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTA                                                                      <  SRR19973066.377266/150‑1 (MQ=60)
                                                                                 GACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGC                                                                    >  SRR19973066.2411589/1‑150 (MQ=60)
                                                                                    GATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATG                                                                 >  SRR19973066.1515788/1‑150 (MQ=60)
                                                                                     ATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGG                                                                >  SRR19973066.2751768/1‑150 (MQ=60)
                                                                                        AACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGA                                                             >  SRR19973066.1418578/1‑150 (MQ=60)
                                                                                         ACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGAT                                                            <  SRR19973066.1677953/150‑1 (MQ=60)
                                                                                          CACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATG                                                           <  SRR19973066.2948420/150‑1 (MQ=60)
                                                                                           ACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGG                                                          >  SRR19973066.2560591/1‑150 (MQ=60)
                                                                                           ACTACTACGCTTCCGTTAACGTTCTGGTTTTGCATAATATTATTTATTGCCCGGAGAGTCTGAAGCTGTATCTTGACTGGCAGGTGGCTATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGG                                                          <  SRR19973066.3304051/150‑1 (MQ=60)
                                                                                               CTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCA                                                      >  SRR19973066.2518321/1‑150 (MQ=60)
                                                                                                TACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAG                                                     <  SRR19973066.1537864/150‑1 (MQ=60)
                                                                                                TACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATGGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAG                                                     <  SRR19973066.1537880/150‑1 (MQ=60)
                                                                                                 ACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGG                                                    <  SRR19973066.272448/150‑1 (MQ=60)
                                                                                                   GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCG                                                  <  SRR19973066.2232823/150‑1 (MQ=60)
                                                                                                   GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCG                                                  <  SRR19973066.2232885/150‑1 (MQ=60)
                                                                                                         GTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAG                                            >  SRR19973066.204995/1‑150 (MQ=60)
                                                                                                            AACGATCTGGTTTTGCAGAATATTATTTAGTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTT                                         <  SRR19973066.1730460/150‑1 (MQ=60)
                                                                                                               GATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATC                                      <  SRR19973066.2382022/150‑1 (MQ=60)
                                                                                                               GATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATC                                      >  SRR19973066.2855618/1‑150 (MQ=60)
                                                                                                                ATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCG                                     >  SRR19973066.3018180/1‑150 (MQ=60)
                                                                                                                ATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCG                                     <  SRR19973066.1602321/150‑1 (MQ=60)
                                                                                                                ATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCG                                     >  SRR19973066.1228492/1‑150 (MQ=60)
                                                                                                                  CTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTANCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGT                                   >  SRR19973066.1235975/1‑150 (MQ=60)
                                                                                                                   TGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTC                                  <  SRR19973066.994868/150‑1 (MQ=60)
                                                                                                                    GGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCA                                 <  SRR19973066.576732/150‑1 (MQ=60)
                                                                                                                       TTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCT                              <  SRR19973066.1431225/150‑1 (MQ=60)
                                                                                                                            AGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGC                         >  SRR19973066.829104/1‑150 (MQ=60)
                                                                                                                              AATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAT                       >  SRR19973066.1608803/1‑150 (MQ=60)
                                                                                                                              AATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAT                       >  SRR19973066.1612816/1‑150 (MQ=60)
                                                                                                                              AATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAT                       >  SRR19973066.50500/1‑150 (MQ=60)
                                                                                                                               ATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATG                      >  SRR19973066.2150400/1‑150 (MQ=60)
                                                                                                                               ATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATG                      >  SRR19973066.2150351/1‑150 (MQ=60)
                                                                                                                                 ATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAA                    <  SRR19973066.2777486/150‑1 (MQ=60)
                                                                                                                                  TTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAA                   >  SRR19973066.2598167/1‑150 (MQ=60)
                                                                                                                                  TTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAA                   >  SRR19973066.2224247/1‑150 (MQ=60)
                                                                                                                                   TATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAG                  <  SRR19973066.794418/150‑1 (MQ=60)
                                                                                                                                      TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973066.3094273/150‑1 (MQ=60)
                                                                                                                                      TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973066.3094433/150‑1 (MQ=60)
                                                                                                                                       TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT              >  SRR19973066.1286263/1‑150 (MQ=60)
                                                                                                                                       TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT              >  SRR19973066.1492212/1‑150 (MQ=60)
                                                                                                                                       TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT              >  SRR19973066.3498979/1‑150 (MQ=60)
                                                                                                                                       TATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGT              >  SRR19973066.3499019/1‑150 (MQ=60)
                                                                                                                                            CCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCA         <  SRR19973066.1973627/150‑1 (MQ=60)
                                                                                                                                             CCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCAT        <  SRR19973066.2452497/150‑1 (MQ=60)
                                                                                                                                              CGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG       <  SRR19973066.1436093/150‑1 (MQ=60)
                                                                                                                                               GGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT      >  SRR19973066.983742/1‑150 (MQ=60)
                                                                                                                                               GGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT      >  SRR19973066.983837/1‑150 (MQ=60)
                                                                                                                                                GAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTG     <  SRR19973066.240025/150‑1 (MQ=60)
                                                                                                                                                  GAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCC   <  SRR19973066.181428/150‑1 (MQ=60)
                                                                                                                                                   AGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  <  SRR19973066.2658289/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  >  CP009273/4088703‑4088999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑