Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,088,850 C→A 100% R93S (CGT→AGT)  rhaR → transcriptional activator of rhaSR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,088,8500CA98.1% 722.0 / 3.4 226R93S (CGT→AGT) rhaRtranscriptional activator of rhaSR
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (132/89);  total (135/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994
                                                                                                                                                 |                                                                                                                                                
  ggCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCt                                                                                                                                              <  2:2562824/148‑1 (MQ=255)
  ggCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCt                                                                                                                                              >  1:1651712/1‑148 (MQ=255)
   gcgcGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTg                                                                                                                                             >  1:1584037/1‑148 (MQ=255)
          aaTGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGa                                                                                                                                      <  2:252424/148‑1 (MQ=255)
          aaTGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGa                                                                                                                                      <  1:2988846/148‑1 (MQ=255)
           aTGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGaa                                                                                                                                     <  2:1915841/148‑1 (MQ=255)
            tGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAt                                                                                                                                    >  2:1499455/1‑148 (MQ=255)
            tGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAt                                                                                                                                    <  1:1715395/148‑1 (MQ=255)
            tGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACACGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAt                                                                                                                                    <  1:3089421/148‑1 (MQ=255)
               ccTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCtt                                                                                                                                 >  2:424913/1‑148 (MQ=255)
                cTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTg                                                                                                                                >  1:3375693/1‑148 (MQ=255)
                 tGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGa                                                                                                                               >  1:1113753/1‑148 (MQ=255)
                  gCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGAc                                                                                                                              >  2:2211549/1‑148 (MQ=255)
                   cATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACt                                                                                                                             >  1:1596973/1‑148 (MQ=255)
                      gTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCAATTACAATCAAGCTGACGATAAACACTCCTACGCTTCCGTTAACCATCTGGTTTTGCAGAATATTATTTATTGCACGTAGAGTCTGAAGCTGAATATTGACTTGc                                                                                                                          >  1:328561/1‑148 (MQ=255)
                         cTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgg                                                                                                                       <  2:2793531/148‑1 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     <  1:540459/148‑1 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     >  1:121517/1‑148 (MQ=255)
                           cAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     >  2:404238/1‑148 (MQ=255)
                           cAACGATCGCCCTTATCGCATGACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAgggg                                                                                                                     <  2:2397838/148‑1 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  1:3272652/1‑148 (MQ=255)
                              cGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCg                                                                                                                  >  1:2464689/1‑148 (MQ=255)
                                aTCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAt                                                                                                                >  1:3102629/1‑148 (MQ=255)
                                 tCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAtt                                                                                                               >  1:2584129/1‑148 (MQ=255)
                                 tCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAtt                                                                                                               >  1:2584656/1‑148 (MQ=255)
                                 tCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAtt                                                                                                               >  1:1973947/1‑148 (MQ=255)
                                    cccTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCg                                                                                                            >  1:347468/1‑148 (MQ=255)
                                     ccTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCgg                                                                                                           >  2:1679667/1‑148 (MQ=255)
                                      cTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCggg                                                                                                          >  1:584724/1‑148 (MQ=255)
                                       ttATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGa                                                                                                         <  1:2781993/148‑1 (MQ=255)
                                        tATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGAt                                                                                                        <  2:2756292/148‑1 (MQ=255)
                                             cATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAc                                                                                                   >  1:1305426/1‑148 (MQ=255)
                                                 aCCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCa                                                                                               <  2:2473296/148‑1 (MQ=255)
                                                  cccGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAg                                                                                              >  1:394089/1‑148 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  1:2897519/1‑148 (MQ=255)
                                                       ggCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAg                                                                                         >  1:75600/1‑148 (MQ=255)
                                                        gCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAgg                                                                                        >  1:587296/1‑148 (MQ=255)
                                                         cGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAggg                                                                                       >  2:2675588/1‑148 (MQ=255)
                                                            tctcTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCaa                                                                                    <  1:1852788/148‑1 (MQ=255)
                                                            tctcTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCaa                                                                                    >  1:1371634/1‑148 (MQ=255)
                                                            tctcTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCaa                                                                                    >  1:660257/1‑148 (MQ=255)
                                                             ctctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAc                                                                                   >  2:1224083/1‑148 (MQ=255)
                                                              tctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAcc                                                                                  <  2:3122462/148‑1 (MQ=255)
                                                              tctTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAAcc                                                                                  <  2:2104825/148‑1 (MQ=255)
                                                                ttttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATATTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcac                                                                                <  1:906368/148‑1 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               <  2:2247112/148‑1 (MQ=255)
                                                                 tttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACcaca                                                                               <  2:394035/148‑1 (MQ=255)
                                                                  ttACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCacac                                                                              >  1:3365557/1‑148 (MQ=255)
                                                                   tACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACt                                                                             >  1:927216/1‑148 (MQ=255)
                                                                   tACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACt                                                                             <  1:2445094/148‑1 (MQ=255)
                                                                    aCATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTg                                                                            <  2:3268356/148‑1 (MQ=255)
                                                                     cATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTgg                                                                           >  1:1197433/1‑148 (MQ=255)
                                                                      aTTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgc                                                                          >  2:315226/1‑148 (MQ=255)
                                                                         cATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGgcgcg                                                                       >  1:3462289/1‑147 (MQ=255)
                                                                         cATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCt                                                                       <  2:540276/148‑1 (MQ=255)
                                                                                    tAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATg                                                            >  1:2201562/1‑148 (MQ=255)
                                                                                        cacTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGa                                                        <  2:2442394/148‑1 (MQ=255)
                                                                                              tACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGgcgc                                                  >  1:464378/1‑148 (MQ=255)
                                                                                               aCGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCa                                                 >  1:1989380/1‑148 (MQ=255)
                                                                                               aCGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCa                                                 <  2:2584136/148‑1 (MQ=255)
                                                                                               aCGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCa                                                 >  2:713707/1‑148 (MQ=255)
                                                                                                cGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAg                                                >  2:1144578/1‑148 (MQ=255)
                                                                                                 gcgtCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAgg                                               <  2:3421826/145‑1 (MQ=255)
                                                                                                 gCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAgg                                               <  1:2279123/148‑1 (MQ=255)
                                                                                                 gCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAgg                                               <  1:1005784/148‑1 (MQ=255)
                                                                                                    tCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGgcgc                                            >  1:2750073/1‑148 (MQ=255)
                                                                                                    tCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGgcgc                                            >  2:1118888/1‑148 (MQ=255)
                                                                                                       gTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGc                                         >  1:1538580/1‑148 (MQ=255)
                                                                                                       gTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGc                                         >  1:181949/1‑148 (MQ=255)
                                                                                                       gTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGc                                         >  1:599947/1‑148 (MQ=255)
                                                                                                         tAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAg                                       <  1:3197647/148‑1 (MQ=255)
                                                                                                          aaCGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAgg                                      >  1:798580/1‑148 (MQ=255)
                                                                                                           aCGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGt                                     <  2:182198/148‑1 (MQ=255)
                                                                                                              aTCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCTCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTAt                                  >  2:2876501/1‑148 (MQ=255)
                                                                                                              aTCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTAt                                  >  2:30443/1‑148 (MQ=255)
                                                                                                               tCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACAGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATc                                 >  2:2630629/1‑148 (MQ=255)
                                                                                                                 tGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCgg                               <  2:328561/148‑1 (MQ=255)
                                                                                                                      ttGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGc                          <  2:790979/148‑1 (MQ=255)
                                                                                                                      ttGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGc                          <  2:1113761/148‑1 (MQ=255)
                                                                                                                        gCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCtt                        <  2:1446539/148‑1 (MQ=255)
                                                                                                                        gCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCtt                        <  2:2448300/148‑1 (MQ=255)
                                                                                                                           gAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAg                     >  2:152369/1‑148 (MQ=255)
                                                                                                                             atatTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTAGACTGGCACGGGGCGATTCCGGGATTAAACGCCAGCGCAGGGCAACCACACAGGCGCTTAGGTAGCATGGGGAAGGCGCAGGCGCGGCAGGTTAGCGGTCAGCGTGAGCa                   >  2:1275505/1‑148 (MQ=255)
                                                                                                                              tattatTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAt                  >  2:2794804/1‑148 (MQ=255)
                                                                                                                              tattatTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAt                  <  2:3202837/148‑1 (MQ=255)
                                                                                                                              tattatTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAt                  >  1:3353580/1‑148 (MQ=255)
                                                                                                                               attattTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGTGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATg                 <  2:2201202/148‑1 (MQ=255)
                                                                                                                                 tatttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaa               <  2:3365725/148‑1 (MQ=255)
                                                                                                                                 tatttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGaa               <  2:3365556/148‑1 (MQ=255)
                                                                                                                                    ttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagt            <  1:1158031/148‑1 (MQ=255)
                                                                                                                                    ttattGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagt            <  1:140575/148‑1 (MQ=255)
                                                                                                                                      attGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtag          <  2:174734/148‑1 (MQ=255)
                                                                                                                                       ttGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAagtagt         <  1:2944202/148‑1 (MQ=255)
                                                                                                                                        tGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTc        >  2:2330277/1‑148 (MQ=255)
                                                                                                                                        tGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTc        >  1:2226596/1‑148 (MQ=255)
                                                                                                                                          cccGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAg      >  1:1813327/1‑148 (MQ=255)
                                                                                                                                           ccGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGc     >  2:627698/1‑148 (MQ=255)
                                                                                                                                            cGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCa    <  1:30428/148‑1 (MQ=255)
                                                                                                                                             ggAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCGTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCAt   >  2:3253338/1‑148 (MQ=255)
                                                                                                                                              gagaGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCAtg  >  2:1560525/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG  >  CP009273/4088705‑4088994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  >  CP009273/4088702‑4088999
                                                                                                                                                    |                                                                                                                                                     
TGTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAG                                                                                                                                                      <  SRR19973065.2512279/150‑1 (MQ=60)
   CTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCT                                                                                                                                                   >  SRR19973065.1662789/1‑150 (MQ=60)
    TGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTG                                                                                                                                                  >  SRR19973065.3343635/1‑150 (MQ=60)
    TGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTG                                                                                                                                                  >  SRR19973065.1594600/1‑150 (MQ=60)
           GTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGA                                                                                                                                           <  SRR19973065.3012976/150‑1 (MQ=60)
             AATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAAT                                                                                                                                         <  SRR19973065.3007140/150‑1 (MQ=60)
               TGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCT                                                                                                                                       <  SRR19973065.3108336/150‑1 (MQ=60)
               TGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCT                                                                                                                                       <  SRR19973065.1726798/150‑1 (MQ=60)
               TGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACACGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCT                                                                                                                                       <  SRR19973065.3108284/150‑1 (MQ=60)
                 GCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTG                                                                                                                                     >  SRR19973065.3396337/1‑150 (MQ=60)
                  CCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGA                                                                                                                                    >  SRR19973065.1121030/1‑150 (MQ=60)
                    TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACTATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                                  >  SRR19973065.2811715/1‑150 (MQ=60)
                    TGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACT                                                                                                                                  >  SRR19973065.1607639/1‑150 (MQ=60)
                       ATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGC                                                                                                                               >  SRR19973065.330764/1‑150 (MQ=60)
                       ATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCAATTACAATCAAGCTGACGATAAACACTCCTACGCTTCCGTTAACCATCTGGTTTTGCAGAATATTATTTATTGCACGTAGAGTCTGAAGCTGAATATTGACTTGC                                                                                                                               >  SRR19973065.330854/1‑150 (MQ=60)
                          TACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGG                                                                                                                            <  SRR19973065.3227565/150‑1 (MQ=60)
                           ACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGG                                                                                                                           >  SRR19973065.3526271/1‑150 (MQ=60)
                            CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          >  SRR19973065.122579/1‑150 (MQ=60)
                            CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          >  SRR19973065.2622314/1‑150 (MQ=60)
                            CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          <  SRR19973065.2532904/150‑1 (MQ=60)
                            CTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGG                                                                                                                          >  SRR19973065.122553/1‑150 (MQ=60)
                              CAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGNC                                                                                                                        <  SRR19973065.543966/150‑1 (MQ=60)
                               AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                       >  SRR19973065.2480224/1‑150 (MQ=60)
                               AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                       >  SRR19973065.3292511/1‑150 (MQ=60)
                               AACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCG                                                                                                                       >  SRR19973065.425024/1‑150 (MQ=60)
                                 CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     >  SRR19973065.1607591/1‑150 (MQ=60)
                                 CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     >  SRR19973065.3124514/1‑150 (MQ=60)
                                 CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     >  SRR19973065.3121586/1‑150 (MQ=60)
                                 CGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGAT                                                                                                                     >  SRR19973065.3121560/1‑150 (MQ=60)
                                  GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    >  SRR19973065.2600229/1‑150 (MQ=60)
                                  GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    >  SRR19973065.1986604/1‑150 (MQ=60)
                                  GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    >  SRR19973065.2600236/1‑150 (MQ=60)
                                  GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    >  SRR19973065.1986800/1‑150 (MQ=60)
                                  GATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATT                                                                                                                    >  SRR19973065.2600757/1‑150 (MQ=60)
                                     CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 >  SRR19973065.349898/1‑150 (MQ=60)
                                     CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 >  SRR19973065.349262/1‑150 (MQ=60)
                                     CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 >  SRR19973065.1609433/1‑150 (MQ=60)
                                     CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 >  SRR19973065.611797/1‑150 (MQ=60)
                                     CGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCG                                                                                                                 >  SRR19973065.611799/1‑150 (MQ=60)
                                       CCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGG                                                                                                               >  SRR19973065.588365/1‑150 (MQ=60)
                                       CCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGG                                                                                                               >  SRR19973065.588385/1‑150 (MQ=60)
                                          TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                            <  SRR19973065.2799251/150‑1 (MQ=60)
                                          TTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATT                                                                                                            >  SRR19973065.1327092/1‑150 (MQ=60)
                                            ATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTA                                                                                                          >  SRR19973065.176126/1‑150 (MQ=60)
                                             TCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAA                                                                                                         <  SRR19973065.2919009/150‑1 (MQ=60)
                                             TCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAA                                                                                                         <  SRR19973065.1158644/150‑1 (MQ=60)
                                              CGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAAC                                                                                                        >  SRR19973065.1314028/1‑150 (MQ=60)
                                                  TTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCA                                                                                                    >  SRR19973065.571236/1‑150 (MQ=60)
                                                   TACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAG                                                                                                   >  SRR19973065.396842/1‑150 (MQ=60)
                                                    ACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGC                                                                                                  >  SRR19973065.3346603/1‑150 (MQ=60)
                                                    ACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGC                                                                                                  >  SRR19973065.801793/1‑150 (MQ=60)
                                                    ACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGC                                                                                                  >  SRR19973065.479223/1‑150 (MQ=60)
                                                        GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                              >  SRR19973065.2915205/1‑150 (MQ=60)
                                                        GTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAG                                                                                              >  SRR19973065.76368/1‑150 (MQ=60)
                                                         TGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGG                                                                                             >  SRR19973065.590948/1‑150 (MQ=60)
                                                             GATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAA                                                                                         >  SRR19973065.664627/1‑150 (MQ=60)
                                                             GATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAA                                                                                         >  SRR19973065.1380689/1‑150 (MQ=60)
                                                               TCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACC                                                                                       >  SRR19973065.2333103/1‑150 (MQ=60)
                                                               TCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACC                                                                                       <  SRR19973065.1864991/150‑1 (MQ=60)
                                                               TCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACC                                                                                       <  SRR19973065.1864973/150‑1 (MQ=60)
                                                                   TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCTTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                                   >  SRR19973065.1519528/1‑150 (MQ=60)
                                                                   TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                                   >  SRR19973065.3386305/1‑150 (MQ=60)
                                                                   TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                                   >  SRR19973065.3386136/1‑150 (MQ=60)
                                                                   TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                                   >  SRR19973065.3386135/1‑150 (MQ=60)
                                                                   TTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATATTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACAC                                                                                   <  SRR19973065.912230/150‑1 (MQ=60)
                                                                    TTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACT                                                                                  >  SRR19973065.933310/1‑150 (MQ=60)
                                                                     TTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTG                                                                                 >  SRR19973065.1196544/1‑150 (MQ=60)
                                                                      TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCACCCACACTGG                                                                                >  SRR19973065.2709634/1‑150 (MQ=60)
                                                                      TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                                <  SRR19973065.2461182/150‑1 (MQ=60)
                                                                      TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                                <  SRR19973065.2460503/150‑1 (MQ=60)
                                                                      TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                                >  SRR19973065.1205068/1‑150 (MQ=60)
                                                                      TACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGG                                                                                <  SRR19973065.1690958/150‑1 (MQ=60)
                                                                       ACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGC                                                                               >  SRR19973065.2987493/1‑150 (MQ=60)
                                                                       ACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGAAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCAGGGATTTATCGCCAGCGCAGGGCAACCACACTGGC                                                                               >  SRR19973065.2986613/1‑150 (MQ=60)
                                                                          TTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCT                                                                            >  SRR19973065.3483434/1‑150 (MQ=60)
                                                                          TTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCG                                                                            >  SRR19973065.3483379/1‑150 (MQ=60)
                                                                                  GACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGC                                                                    >  SRR19973065.1630609/1‑150 (MQ=60)
                                                                                     GATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATG                                                                 >  SRR19973065.2215662/1‑150 (MQ=60)
                                                                                     GATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATG                                                                 >  SRR19973065.2215659/1‑150 (MQ=60)
                                                                                               CCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCCGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGC                                                       >  SRR19973065.467582/1‑150 (MQ=60)
                                                                                               CCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGC                                                       >  SRR19973065.2245582/1‑150 (MQ=60)
                                                                                                CTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCA                                                      >  SRR19973065.2002364/1‑150 (MQ=60)
                                                                                                CTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCA                                                      >  SRR19973065.2002287/1‑150 (MQ=60)
                                                                                                 TACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTTAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAG                                                     >  SRR19973065.924138/1‑150 (MQ=60)
                                                                                                    GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCG                                                  <  SRR19973065.2293499/150‑1 (MQ=60)
                                                                                                    GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCG                                                  <  SRR19973065.1012461/150‑1 (MQ=60)
                                                                                                     CTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGC                                                 >  SRR19973065.2767187/1‑150 (MQ=60)
                                                                                                     CTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGC                                                 >  SRR19973065.2767323/1‑150 (MQ=60)
                                                                                                        CCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGC                                              >  SRR19973065.603697/1‑150 (MQ=60)
                                                                                                        CCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGC                                              >  SRR19973065.603305/1‑150 (MQ=60)
                                                                                                        CCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGC                                              >  SRR19973065.1548736/1‑150 (MQ=60)
                                                                                                        CCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGC                                              >  SRR19973065.183388/1‑150 (MQ=60)
                                                                                                        CCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGC                                              >  SRR19973065.183638/1‑150 (MQ=60)
                                                                                                          tttaccgaacgGGTTTGGCAGAATTTTATTTTTGGCCCGGCGCGTCGGACGCTGAACCTTGACGGGCGGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAG                                            <  SRR19973065.1046223/139‑1 (MQ=60)
                                                                                                           TTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGG                                           >  SRR19973065.809114/1‑150 (MQ=60)
                                                                                                           TTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGG                                           >  SRR19973065.803857/1‑150 (MQ=60)
                                                                                                           TTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGG                                           <  SRR19973065.2053339/150‑1 (MQ=60)
                                                                                                            TAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGT                                          <  SRR19973065.3216912/150‑1 (MQ=60)
                                                                                                                GATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATC                                      >  SRR19973065.832429/1‑150 (MQ=60)
                                                                                                                 ATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCG                                     <  SRR19973065.509432/150‑1 (MQ=60)
                                                                                                                               AATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCAT                       >  SRR19973065.3374074/1‑150 (MQ=60)
                                                                                                                                ATATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGAAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATG                      <  SRR19973065.1215247/150‑1 (MQ=60)
                                                                                                                                 TATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGA                     >  SRR19973065.155942/1‑150 (MQ=60)
                                                                                                                                  ATTATTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAA                    >  SRR19973065.1296768/1‑150 (MQ=60)
                                                                                                                                      TTTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTA                <  SRR19973065.2474105/150‑1 (MQ=60)
                                                                                                                                       TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973065.1546449/150‑1 (MQ=60)
                                                                                                                                       TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973065.1165458/150‑1 (MQ=60)
                                                                                                                                       TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973065.141544/150‑1 (MQ=60)
                                                                                                                                       TTATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAG               <  SRR19973065.141777/150‑1 (MQ=60)
                                                                                                                                         ATTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTC             >  SRR19973065.2240781/1‑150 (MQ=60)
                                                                                                                                          TTGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCA            <  SRR19973065.2962092/150‑1 (MQ=60)
                                                                                                                                           TGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAG           >  SRR19973065.1825266/1‑150 (MQ=60)
                                                                                                                                           TGCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAG           >  SRR19973065.1826047/1‑150 (MQ=60)
                                                                                                                                            GCCCGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGC          >  SRR19973065.1996149/1‑150 (MQ=60)
                                                                                                                                               CGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG       <  SRR19973065.3456755/150‑1 (MQ=60)
                                                                                                                                               CGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG       <  SRR19973065.30817/150‑1 (MQ=60)
                                                                                                                                               CGGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATG       <  SRR19973065.30832/150‑1 (MQ=60)
                                                                                                                                                GGAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGT      >  SRR19973065.120542/1‑150 (MQ=60)
                                                                                                                                                 GAGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTG     <  SRR19973065.694913/150‑1 (MQ=60)
                                                                                                                                                  AGAGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGC    <  SRR19973065.2349047/150‑1 (MQ=60)
                                                                                                                                                    AGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  >  SRR19973065.2466116/1‑150 (MQ=60)
                                                                                                                                                    AGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  <  SRR19973065.827884/150‑1 (MQ=60)
                                                                                                                                                    AGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  <  SRR19973065.427872/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGTCTGGCGCGGTAATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACACTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCG  >  CP009273/4088702‑4088999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑