Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,020 T→A 100% E137V (GAA→GTA)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,0200TA97.7% 696.2 / 4.3 216E137V (GAA→GTA) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base A (108/103);  total (109/107)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT  >  CP009273/494875‑495164
                                                                                                                                                 |                                                                                                                                                
gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTAcc                                                                                                                                                <  1:457442/148‑1 (MQ=255)
 cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACct                                                                                                                                               <  1:635664/148‑1 (MQ=255)
 cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACct                                                                                                                                               <  1:3142283/148‑1 (MQ=255)
  aaTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctc                                                                                                                                              >  2:448012/1‑148 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                            >  1:920134/1‑148 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                            <  1:202951/148‑1 (MQ=255)
     cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTAt                                                                                                                                           <  2:2512493/148‑1 (MQ=255)
       aTCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCg                                                                                                                                         >  1:3345165/1‑148 (MQ=255)
        tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGc                                                                                                                                        <  2:1616036/148‑1 (MQ=255)
        tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGc                                                                                                                                        >  1:2530289/1‑148 (MQ=255)
         cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                       >  2:30025/1‑148 (MQ=255)
         cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                       >  2:1850915/1‑148 (MQ=255)
           gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTg                                                                                                                                     <  2:2188403/148‑1 (MQ=255)
           gTTGATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTg                                                                                                                                     <  2:1016925/148‑1 (MQ=255)
            tttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGc                                                                                                                                    >  1:1250162/1‑148 (MQ=255)
             ttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCg                                                                                                                                   <  1:1179870/148‑1 (MQ=255)
                tCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaa                                                                                                                                >  1:2935722/1‑148 (MQ=255)
                tCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaa                                                                                                                                >  1:2935429/1‑148 (MQ=255)
                 cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCCATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaa                                                                                                                               >  2:190272/1‑148 (MQ=255)
                 cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaa                                                                                                                               >  2:190266/1‑148 (MQ=255)
                  aCGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaaa                                                                                                                              <  1:49443/148‑1 (MQ=255)
                  aCGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaaa                                                                                                                              >  1:2901030/1‑148 (MQ=255)
                    gCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAcg                                                                                                                            <  1:3242818/148‑1 (MQ=255)
                    gCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAcg                                                                                                                            <  1:1813870/148‑1 (MQ=255)
                     cAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAcgc                                                                                                                           <  2:849969/148‑1 (MQ=255)
                      aaCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAcgcg                                                                                                                          <  1:3170890/148‑1 (MQ=255)
                       aCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACgcgc                                                                                                                         >  1:349094/1‑148 (MQ=255)
                        ccACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGACATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCt                                                                                                                        >  1:3451416/1‑148 (MQ=255)
                           caACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                     >  1:1293855/1‑148 (MQ=255)
                           caACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                     <  1:3310059/148‑1 (MQ=255)
                            aaCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAg                                                                                                                    <  1:3204066/148‑1 (MQ=255)
                             aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGAAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                   <  2:3375982/148‑1 (MQ=255)
                                  cTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGaaa                                                                                                              >  1:2531839/1‑148 (MQ=255)
                                    ggCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAgg                                                                                                            >  1:2667579/1‑148 (MQ=255)
                                     gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAggg                                                                                                           >  1:2147987/1‑148 (MQ=255)
                                       gAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgtg                                                                                                         <  1:133946/148‑1 (MQ=255)
                                        aGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgtgt                                                                                                        <  2:321330/148‑1 (MQ=255)
                                         gcgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAACACGCGCTTCAGGTGAAAGGGTGTa                                                                                                       >  2:2578442/1‑148 (MQ=255)
                                         gcgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTa                                                                                                       <  1:2921998/148‑1 (MQ=255)
                                               gCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAt                                                                                                 <  1:2454520/148‑1 (MQ=255)
                                                    gCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCaa                                                                                            >  1:1579802/1‑148 (MQ=255)
                                                       ccTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCa                                                                                         <  2:1645095/148‑1 (MQ=255)
                                                         tGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCAcc                                                                                       <  2:1966984/148‑1 (MQ=255)
                                                          gCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCg                                                                                      <  2:2946028/148‑1 (MQ=255)
                                                             gAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAc                                                                                   <  2:2890020/148‑1 (MQ=255)
                                                             gAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAc                                                                                   >  1:253282/1‑148 (MQ=255)
                                                              aaTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACa                                                                                  <  1:3089814/148‑1 (MQ=255)
                                                                      gCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGt                                                                          <  2:282878/148‑1 (MQ=255)
                                                                          aGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCt                                                                      >  1:1900119/1‑148 (MQ=255)
                                                                          aGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCt                                                                      >  2:557944/1‑148 (MQ=255)
                                                                          aGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCt                                                                      >  2:358971/1‑148 (MQ=255)
                                                                             cAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGc                                                                   <  2:2608918/148‑1 (MQ=255)
                                                                             cAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGc                                                                   >  1:1978834/1‑148 (MQ=255)
                                                                                    ggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAgg                                                            >  1:1407064/1‑148 (MQ=255)
                                                                                    ggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAgg                                                            >  1:2481323/1‑148 (MQ=255)
                                                                                      aCATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGgcg                                                          >  1:3550022/1‑148 (MQ=255)
                                                                                         tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                       >  1:737817/1‑148 (MQ=255)
                                                                                         tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                       >  2:3179438/1‑148 (MQ=255)
                                                                                          atTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                      <  2:3317811/148‑1 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                     <  2:1821898/148‑1 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                     <  2:2095966/148‑1 (MQ=255)
                                                                                               tttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCg                                                 >  2:1418520/1‑148 (MQ=255)
                                                                                                ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                >  1:382504/1‑148 (MQ=255)
                                                                                                ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                >  2:741697/1‑148 (MQ=255)
                                                                                                 tgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGAt                                               >  1:95979/1‑148 (MQ=255)
                                                                                                  gatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATc                                              <  2:1352408/148‑1 (MQ=255)
                                                                                                      aTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTgg                                          <  2:2066124/148‑1 (MQ=255)
                                                                                                      aTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTgg                                          <  1:2979629/148‑1 (MQ=255)
                                                                                                       tcctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTggg                                         <  1:1488567/148‑1 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                        >  2:1169999/1‑148 (MQ=255)
                                                                                                         ctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTa                                       <  2:3083879/148‑1 (MQ=255)
                                                                                                          tccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAt                                      <  2:1497060/148‑1 (MQ=255)
                                                                                                           ccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATc                                     >  1:2550355/1‑148 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                  >  1:233682/1‑148 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                  >  1:1652192/1‑148 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                  >  1:1717389/1‑148 (MQ=255)
                                                                                                                  ctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTg                              <  1:2942222/148‑1 (MQ=255)
                                                                                                                     gtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                           >  1:1877858/1‑148 (MQ=255)
                                                                                                                     gtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                           <  1:311415/148‑1 (MQ=255)
                                                                                                                        gAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgaga                        >  2:2809301/1‑148 (MQ=255)
                                                                                                                         aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                       <  2:3541925/148‑1 (MQ=255)
                                                                                                                         aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                       <  2:3494917/148‑1 (MQ=255)
                                                                                                                           aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                     >  2:1181951/1‑148 (MQ=255)
                                                                                                                           aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                     >  2:2336390/1‑148 (MQ=255)
                                                                                                                            tAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaa                    >  2:544993/1‑148 (MQ=255)
                                                                                                                             aacaacAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                   >  1:2039219/1‑148 (MQ=255)
                                                                                                                                 acaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCg               <  1:448013/148‑1 (MQ=255)
                                                                                                                                 acaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCg               >  1:896521/1‑148 (MQ=255)
                                                                                                                                  caaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGc              >  1:2982246/1‑148 (MQ=255)
                                                                                                                                   aaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGcc             <  2:831772/148‑1 (MQ=255)
                                                                                                                                     gcagcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTc           <  2:2716751/148‑1 (MQ=255)
                                                                                                                                      cagcCACATTTACCTCTATCGCTTGCGGAAAACGCGCTTCTGGTGAAAGGGTGTAATCAATACCAATCACCGTACTTTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTcc          <  2:920134/148‑1 (MQ=255)
                                                                                                                                       agcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGACTAAAACCGCCTCCa         >  2:885902/1‑148 (MQ=255)
                                                                                                                                       agcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCa         >  2:2666842/1‑148 (MQ=255)
                                                                                                                                         ccACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATg       >  2:66679/1‑148 (MQ=255)
                                                                                                                                         ccACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATg       >  1:78032/1‑148 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa    >  2:3188214/1‑148 (MQ=255)
                                                                                                                                             aaTTACCTCTATCGCTTGCTGAAAACGCTCTTCTGGTGAAAGGGTGTAATCAATACCAATCACCGTACTTTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATGGTGGGTATCGAGTTTGCCGACAATAAAACCGCCTCCATGCaaa   <  1:613188/148‑1 (MQ=255)
                                                                                                                                              aTTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaaat  <  2:2481323/148‑1 (MQ=255)
                                                                                                                                              aTTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaaat  >  1:2727556/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT  >  CP009273/494875‑495164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAA  >  CP009273/494871‑495166
                                                                                                                                                     |                                                                                                                                                  
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTA                                                                                                                                                    >  SRR19973067.2962489/1‑150 (MQ=60)
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTA                                                                                                                                                    >  SRR19973067.602010/1‑150 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                  <  SRR19973067.627006/150‑1 (MQ=60)
   CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCT                                                                                                                                                 >  SRR19973067.607322/1‑150 (MQ=60)
    GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTC                                                                                                                                                <  SRR19973067.460609/150‑1 (MQ=60)
     CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCT                                                                                                                                               <  SRR19973067.2379190/150‑1 (MQ=60)
     CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCT                                                                                                                                               <  SRR19973067.3162230/150‑1 (MQ=60)
     CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCT                                                                                                                                               <  SRR19973067.639937/150‑1 (MQ=60)
      AATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTA                                                                                                                                              >  SRR19973067.926294/1‑150 (MQ=60)
        TCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATC                                                                                                                                            <  SRR19973067.204493/150‑1 (MQ=60)
         CGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCG                                                                                                                                           >  SRR19973067.3366361/1‑150 (MQ=60)
         CGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCG                                                                                                                                           >  SRR19973067.3366319/1‑150 (MQ=60)
          GATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGC                                                                                                                                          >  SRR19973067.2546855/1‑150 (MQ=60)
          GATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGC                                                                                                                                          >  SRR19973067.2546868/1‑150 (MQ=60)
           ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCT                                                                                                                                         >  SRR19973067.45757/1‑150 (MQ=60)
              TGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGC                                                                                                                                      >  SRR19973067.1258605/1‑150 (MQ=60)
                 TTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGA                                                                                                                                   <  SRR19973067.1187664/150‑1 (MQ=60)
                  TATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAA                                                                                                                                  >  SRR19973067.2954053/1‑150 (MQ=60)
                  TATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAA                                                                                                                                  >  SRR19973067.2954299/1‑150 (MQ=60)
                  TATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAA                                                                                                                                  >  SRR19973067.2954348/1‑150 (MQ=60)
                   ATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAA                                                                                                                                 >  SRR19973067.690848/1‑150 (MQ=60)
                    TCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAA                                                                                                                                >  SRR19973067.2919532/1‑150 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                               >  SRR19973067.2548408/1‑150 (MQ=60)
                      ACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACG                                                                                                                              <  SRR19973067.1349326/150‑1 (MQ=60)
                      ACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACG                                                                                                                              <  SRR19973067.3128513/150‑1 (MQ=60)
                      ACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACG                                                                                                                              <  SRR19973067.50096/150‑1 (MQ=60)
                        GCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCG                                                                                                                            <  SRR19973067.3262982/150‑1 (MQ=60)
                        GCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCG                                                                                                                            <  SRR19973067.1826146/150‑1 (MQ=60)
                        GCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCG                                                                                                                            <  SRR19973067.3263138/150‑1 (MQ=60)
                         CAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGC                                                                                                                           >  SRR19973067.351505/1‑150 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGACATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                          >  SRR19973067.3473371/1‑150 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                          <  SRR19973067.3190573/150‑1 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                          <  SRR19973067.3486059/150‑1 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                          <  SRR19973067.3190966/150‑1 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                          >  SRR19973067.2980084/1‑150 (MQ=60)
                             CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                       >  SRR19973067.1302685/1‑150 (MQ=60)
                             CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                       >  SRR19973067.1302870/1‑150 (MQ=60)
                               CAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGG                                                                                                                     <  SRR19973067.3330834/150‑1 (MQ=60)
                               CAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGG                                                                                                                     <  SRR19973067.3330958/150‑1 (MQ=60)
                               CAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGG                                                                                                                     <  SRR19973067.3330889/150‑1 (MQ=60)
                                ACCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                    <  SRR19973067.3224258/150‑1 (MQ=60)
                                AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                    <  SRR19973067.3224265/150‑1 (MQ=60)
                                    CACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAA                                                                                                                >  SRR19973067.2548425/1‑150 (MQ=60)
                                    CACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAA                                                                                                                >  SRR19973067.2548436/1‑150 (MQ=60)
                                    CACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAA                                                                                                                >  SRR19973067.1731404/1‑150 (MQ=60)
                                      CTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGG                                                                                                              >  SRR19973067.2684715/1‑150 (MQ=60)
                                       TGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGG                                                                                                             >  SRR19973067.2162348/1‑150 (MQ=60)
                                        GGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGT                                                                                                            <  SRR19973067.90360/150‑1 (MQ=60)
                                           GAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTA                                                                                                         <  SRR19973067.135149/150‑1 (MQ=60)
                                             GCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGTGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAAT                                                                                                       <  SRR19973067.2939205/150‑1 (MQ=60)
                                             GCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAACGGGTGTAAT                                                                                                       <  SRR19973067.2940751/150‑1 (MQ=60)
                                             GCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAAT                                                                                                       <  SRR19973067.2940575/150‑1 (MQ=60)
                                                  AGCATGGCACCTGCCGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATA                                                                                                  >  SRR19973067.915722/1‑150 (MQ=60)
                                                   GCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATAC                                                                                                 <  SRR19973067.2470656/150‑1 (MQ=60)
                                                      TGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAA                                                                                              >  SRR19973067.1590617/1‑150 (MQ=60)
                                                        GCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATC                                                                                            <  SRR19973067.3515324/150‑1 (MQ=60)
                                                           CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                         <  SRR19973067.964791/150‑1 (MQ=60)
                                                               CGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAC                                                                                     >  SRR19973067.2825653/1‑150 (MQ=60)
                                                               CGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAC                                                                                     >  SRR19973067.255103/1‑150 (MQ=60)
                                                               CGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAC                                                                                     >  SRR19973067.255020/1‑150 (MQ=60)
                                                                  AATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATT                                                                                  <  SRR19973067.3109472/150‑1 (MQ=60)
                                                                            AAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCT                                                                        <  SRR19973067.1987027/150‑1 (MQ=60)
                                                                            AAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCT                                                                        >  SRR19973067.1913078/1‑150 (MQ=60)
                                                                              AGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTG                                                                      >  SRR19973067.4670/1‑150 (MQ=60)
                                                                               GCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGC                                                                     >  SRR19973067.1992438/1‑150 (MQ=60)
                                                                               GCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGC                                                                     >  SRR19973067.1992237/1‑150 (MQ=60)
                                                                                     GCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTCTCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAG                                                               <  SRR19973067.2001193/150‑1 (MQ=60)
                                                                                     GCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAG                                                               <  SRR19973067.1693610/150‑1 (MQ=60)
                                                                                      CGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGG                                                              >  SRR19973067.1416599/1‑150 (MQ=60)
                                                                                      CGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGG                                                              >  SRR19973067.2497636/1‑150 (MQ=60)
                                                                                        GGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCG                                                            >  SRR19973067.3572412/1‑150 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                         >  SRR19973067.742980/1‑150 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                         >  SRR19973067.742938/1‑150 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                         >  SRR19973067.2909623/1‑150 (MQ=60)
                                                                                              ATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGAT                                                      <  SRR19973067.2541823/150‑1 (MQ=60)
                                                                                                  ATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGA                                                  >  SRR19973067.385168/1‑150 (MQ=60)
                                                                                                  ATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGA                                                  >  SRR19973067.385037/1‑150 (MQ=60)
                                                                                                   TTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGAT                                                 >  SRR19973067.96976/1‑150 (MQ=60)
                                                                                                   TTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGAT                                                 >  SRR19973067.1907609/1‑150 (MQ=60)
                                                                                                       ATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTG                                             >  SRR19973067.434339/1‑150 (MQ=60)
                                                                                                        TAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACGATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGG                                            >  SRR19973067.3399361/1‑150 (MQ=60)
                                                                                                         AATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGG                                           >  SRR19973067.3072775/1‑150 (MQ=60)
                                                                                                         AATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGG                                           >  SRR19973067.2734262/1‑150 (MQ=60)
                                                                                                          ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                          <  SRR19973067.2998642/150‑1 (MQ=60)
                                                                                                          ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                          <  SRR19973067.2998445/150‑1 (MQ=60)
                                                                                                           TCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTA                                         <  SRR19973067.1498423/150‑1 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAATCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGACAGCAGGCGCATGATGCGATCGTGGGTATC                                       >  SRR19973067.2497441/1‑150 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCTTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                       >  SRR19973067.2567042/1‑150 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                       >  SRR19973067.2567036/1‑150 (MQ=60)
                                                                                                              TCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCGTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCG                                      <  SRR19973067.1259664/150‑1 (MQ=60)
                                                                                                                CGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAG                                    >  SRR19973067.1729159/1‑150 (MQ=60)
                                                                                                                CGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAG                                    >  SRR19973067.235341/1‑150 (MQ=60)
                                                                                                                CGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAG                                    >  SRR19973067.1663570/1‑150 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGA                                   <  SRR19973067.2308508/150‑1 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGTAGGCGCATGATGCGATCGTGGGTATCGAGAT                                  <  SRR19973067.1560080/150‑1 (MQ=60)
                                                                                                                     GCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGC                               <  SRR19973067.1616483/150‑1 (MQ=60)
                                                                                                                      CTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCC                              <  SRR19973067.2959335/150‑1 (MQ=60)
                                                                                                                      CTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCC                              <  SRR19973067.2960869/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGTCG                             <  SRR19973067.2043420/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                             >  SRR19973067.1890670/1‑150 (MQ=60)
                                                                                                                         GTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAG                           <  SRR19973067.313466/150‑1 (MQ=60)
                                                                                                                         GTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAG                           <  SRR19973067.313470/150‑1 (MQ=60)
                                                                                                                           GGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAA                         >  SRR19973067.1451194/1‑150 (MQ=60)
                                                                                                                             AAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATA                       <  SRR19973067.30487/150‑1 (MQ=60)
                                                                                                                               ATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAA                     >  SRR19973067.2052864/1‑150 (MQ=60)
                                                                                                                                   CAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                 >  SRR19973067.902440/1‑150 (MQ=60)
                                                                                                                                    AACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGC                >  SRR19973067.3001086/1‑150 (MQ=60)
                                                                                                                                     ACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCC               <  SRR19973067.451119/150‑1 (MQ=60)
                                                                                                                                     ACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCC               <  SRR19973067.451118/150‑1 (MQ=60)
                                                                                                                                      CAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT              >  SRR19973067.2155298/1‑150 (MQ=60)
                                                                                                                                           AGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATG         >  SRR19973067.78810/1‑150 (MQ=60)
                                                                                                                                           AGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATG         >  SRR19973067.78907/1‑150 (MQ=60)
                                                                                                                                           AGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATG         >  SRR19973067.1889047/1‑150 (MQ=60)
                                                                                                                                           AGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATG         >  SRR19973067.1224956/1‑150 (MQ=60)
                                                                                                                                                CAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT    >  SRR19973067.2745156/1‑150 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGGTGGCGTAAAACGCGCTTCTGGTGAATGGGTGTAATAAATACCAATCACCGTACTTTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATGGTGGTTATCGATATTGCCGAGAATAAAACCGCCTCCATGCAAATA   <  SRR19973067.617620/150‑1 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCTGAAAACGCTCTTCTGGTGAAAGGGTGTAATCAATACCAATCACCGTACTTTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATGGTGGGTATCGAGTTTGCCGACAATAAAACCGCCTCCATGCAAATA   <  SRR19973067.617171/150‑1 (MQ=60)
                                                                                                                                                  ATTACCTCTATCGCTTGCGGAAAACGCGCTTCTGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAA  <  SRR19973067.1614929/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAA  >  CP009273/494871‑495166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑