Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 495,020 T→A E137V (GAA→GTA)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,0200TA99.6% 800.1 / NA 244E137V (GAA→GTA) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (100/143);  minor base C (0/1);  total (100/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA  >  CP009273/494873‑495165
                                                                                                                                                   |                                                                                                                                                 
  gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctc                                                                                                                                               <  2:759561/150‑1 (MQ=255)
  gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctc                                                                                                                                               >  1:3476044/1‑150 (MQ=255)
   cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctct                                                                                                                                              <  2:402378/150‑1 (MQ=255)
   cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctct                                                                                                                                              >  2:3565712/1‑150 (MQ=255)
    aaTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                             >  2:1087760/1‑150 (MQ=255)
    aaTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                             >  2:343306/1‑150 (MQ=255)
      tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATc                                                                                                                                           <  1:377595/150‑1 (MQ=255)
       cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCg                                                                                                                                          <  2:1400433/150‑1 (MQ=255)
       cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCg                                                                                                                                          <  2:1954457/150‑1 (MQ=255)
        gATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGc                                                                                                                                         >  1:3415580/1‑150 (MQ=255)
          tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCtt                                                                                                                                       <  1:1598951/150‑1 (MQ=255)
          tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCtt                                                                                                                                       <  1:1880142/150‑1 (MQ=255)
           cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTg                                                                                                                                      <  2:2612532/150‑1 (MQ=255)
                   cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAc                                                                                                                              <  1:1769629/150‑1 (MQ=255)
                    aCGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGTCATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAcg                                                                                                                             >  2:535748/1‑150 (MQ=255)
                       cAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACgcgc                                                                                                                          <  1:2675416/150‑1 (MQ=255)
                           cacaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                      >  2:626185/1‑150 (MQ=255)
                           cacaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTACa                                                                                                                      >  2:2602215/1‑150 (MQ=255)
                              aaCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGt                                                                                                                   <  1:136646/150‑1 (MQ=255)
                               aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTg                                                                                                                  <  1:3024059/150‑1 (MQ=255)
                                cGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGa                                                                                                                 <  2:1580774/150‑1 (MQ=255)
                                      ggCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt                                                                                                           >  1:1150112/1‑150 (MQ=255)
                                         gAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTa                                                                                                        >  1:1960178/1‑150 (MQ=255)
                                            cgcCAGCATGTCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATc                                                                                                     <  2:3114626/150‑1 (MQ=255)
                                            cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATc                                                                                                     <  2:1694351/150‑1 (MQ=255)
                                             gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGGTAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                    <  1:152385/150‑1 (MQ=255)
                                             gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                    <  1:1377174/150‑1 (MQ=255)
                                             gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                    <  1:152366/150‑1 (MQ=255)
                                             gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                    >  1:572047/1‑150 (MQ=255)
                                             gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                    >  2:3476075/1‑150 (MQ=255)
                                              ccAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGAGAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCaa                                                                                                   <  1:1626483/150‑1 (MQ=255)
                                                  cATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACAGATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATAcc                                                                                               <  2:653991/150‑1 (MQ=255)
                                                   aTGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                              >  2:249021/1‑150 (MQ=255)
                                                   aTGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                              >  2:364927/1‑150 (MQ=255)
                                                   aTGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                              <  2:1139622/150‑1 (MQ=255)
                                                    tGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCaa                                                                                             >  1:3103658/1‑150 (MQ=255)
                                                    tGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCaa                                                                                             <  1:2143747/150‑1 (MQ=255)
                                                     ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAt                                                                                            <  1:1918607/150‑1 (MQ=255)
                                                     ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAt                                                                                            <  1:3565712/150‑1 (MQ=255)
                                                       cACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCa                                                                                          <  2:3477613/150‑1 (MQ=255)
                                                         ccTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCAcc                                                                                        <  1:3646338/150‑1 (MQ=255)
                                                         ccTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCAcc                                                                                        <  1:2800698/150‑1 (MQ=255)
                                                          cTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCg                                                                                       >  2:3722471/1‑150 (MQ=255)
                                                           tGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGt                                                                                      <  2:381789/150‑1 (MQ=255)
                                                            gCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTa                                                                                     >  1:707395/1‑150 (MQ=255)
                                                             cGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAc                                                                                    <  2:560289/150‑1 (MQ=255)
                                                              ggAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACa                                                                                   >  2:2203912/1‑150 (MQ=255)
                                                              ggAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACa                                                                                   >  2:1855786/1‑150 (MQ=255)
                                                                  tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTgg                                                                               <  1:1722409/150‑1 (MQ=255)
                                                                    aCCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCt                                                                             <  1:450594/150‑1 (MQ=255)
                                                                     ccGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTg                                                                            <  2:2454563/150‑1 (MQ=255)
                                                                       ggCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTa                                                                          <  1:3682688/150‑1 (MQ=255)
                                                                           aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCtt                                                                      <  1:1046679/150‑1 (MQ=255)
                                                                           aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCtt                                                                      <  1:2948852/150‑1 (MQ=255)
                                                                            aGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTg                                                                     >  2:3363302/1‑150 (MQ=255)
                                                                             gCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGc                                                                    >  2:1624752/1‑150 (MQ=255)
                                                                              ccAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGcc                                                                   >  2:2677202/1‑150 (MQ=255)
                                                                               cAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCca                                                                  >  2:2875805/1‑150 (MQ=255)
                                                                                  tGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCACTCACCGTACATTGGCTGTAGCTTGCcagca                                                               <  2:721235/150‑1 (MQ=255)
                                                                                   gCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcagcag                                                              <  2:3006960/150‑1 (MQ=255)
                                                                                     gggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGgc                                                            >  2:1916362/1‑150 (MQ=255)
                                                                                         cATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                        <  1:3624261/150‑1 (MQ=255)
                                                                                          atatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                       <  2:2374392/150‑1 (MQ=255)
                                                                                           tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                      >  2:2325342/1‑150 (MQ=255)
                                                                                           tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                      >  2:2742866/1‑150 (MQ=255)
                                                                                             ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatgatg                                                    >  1:909455/1‑150 (MQ=255)
                                                                                                atttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                 >  2:3268502/1‑150 (MQ=255)
                                                                                                   tgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCg                                              >  1:3229356/1‑150 (MQ=255)
                                                                                                     atAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                            <  2:3666393/150‑1 (MQ=255)
                                                                                                     atAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                            <  2:3295049/150‑1 (MQ=255)
                                                                                                     atAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                            <  1:1159811/150‑1 (MQ=255)
                                                                                                          cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAt                                       >  1:3172771/1‑150 (MQ=255)
                                                                                                          cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAt                                       <  1:2397907/150‑1 (MQ=255)
                                                                                                           ctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATc                                      <  1:2016230/150‑1 (MQ=255)
                                                                                                               gCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                                  >  2:2214166/1‑150 (MQ=255)
                                                                                                               gCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                                  >  1:3282144/1‑150 (MQ=255)
                                                                                                                ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                                 >  2:873805/1‑150 (MQ=255)
                                                                                                                ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                                 >  2:3199266/1‑150 (MQ=255)
                                                                                                                ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                                 <  2:1535231/150‑1 (MQ=255)
                                                                                                                  tgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTg                               <  2:806744/150‑1 (MQ=255)
                                                                                                                    ctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGcc                             <  2:282926/150‑1 (MQ=255)
                                                                                                                    ctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGcc                             >  2:3164324/1‑150 (MQ=255)
                                                                                                                     tggtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                            <  1:3639515/150‑1 (MQ=255)
                                                                                                                        tggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgaga                         >  1:773794/1‑150 (MQ=255)
                                                                                                                         ggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                        <  1:525845/150‑1 (MQ=255)
                                                                                                                          gAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAAt                       <  1:249021/150‑1 (MQ=255)
                                                                                                                           aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                      >  1:2103028/1‑150 (MQ=255)
                                                                                                                            aaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaa                     <  1:499221/150‑1 (MQ=255)
                                                                                                                             aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                    >  1:1502508/1‑150 (MQ=255)
                                                                                                                             aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                    >  1:392881/1‑150 (MQ=255)
                                                                                                                               aacaacAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                  <  1:3332587/150‑1 (MQ=255)
                                                                                                                                acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAcc                 <  1:1936119/150‑1 (MQ=255)
                                                                                                                                acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAcc                 <  2:2447638/150‑1 (MQ=255)
                                                                                                                                acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAcc                 <  1:110190/150‑1 (MQ=255)
                                                                                                                                  aacaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGc               >  1:2585743/1‑150 (MQ=255)
                                                                                                                                      agcagcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTcc           >  1:2830119/1‑150 (MQ=255)
                                                                                                                                       gcagcCAAAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCa          <  1:2820995/150‑1 (MQ=255)
                                                                                                                                            cacaATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa     >  1:102669/1‑150 (MQ=255)
                                                                                                                                             acaATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTACCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaaa    <  2:3770241/150‑1 (MQ=255)
                                                                                                                                               aaTTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaaata  <  1:876467/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA  >  CP009273/494873‑495165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494871‑495169
                                                                                                                                                     |                                                                                                                                                     
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTA                                                                                                                                                       <  SRR19973061.3773996/150‑1 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                     <  SRR19973061.2058108/150‑1 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                     <  SRR19973061.2058105/150‑1 (MQ=60)
   CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCT                                                                                                                                                    <  SRR19973061.2520158/150‑1 (MQ=60)
   CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCT                                                                                                                                                    <  SRR19973061.2520106/150‑1 (MQ=60)
    GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTC                                                                                                                                                   >  SRR19973061.3484851/1‑150 (MQ=60)
    GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTC                                                                                                                                                   >  SRR19973061.3484939/1‑150 (MQ=60)
     CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCT                                                                                                                                                  <  SRR19973061.2146459/150‑1 (MQ=60)
     CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCT                                                                                                                                                  <  SRR19973061.2700419/150‑1 (MQ=60)
       ATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTAT                                                                                                                                                <  SRR19973061.282141/150‑1 (MQ=60)
        TCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATC                                                                                                                                               <  SRR19973061.378625/150‑1 (MQ=60)
          GATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGC                                                                                                                                             >  SRR19973061.3424347/1‑150 (MQ=60)
            TCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTT                                                                                                                                           <  SRR19973061.1884818/150‑1 (MQ=60)
            TCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTT                                                                                                                                           <  SRR19973061.593247/150‑1 (MQ=60)
            TCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTT                                                                                                                                           <  SRR19973061.1602924/150‑1 (MQ=60)
            TCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTT                                                                                                                                           <  SRR19973061.1071910/150‑1 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          >  SRR19973061.3490668/1‑150 (MQ=60)
               GTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCG                                                                                                                                        <  SRR19973061.1560017/150‑1 (MQ=60)
               GTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCG                                                                                                                                        <  SRR19973061.2170425/150‑1 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                                  <  SRR19973061.1222746/150‑1 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                                  <  SRR19973061.1774062/150‑1 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                                  <  SRR19973061.1773998/150‑1 (MQ=60)
                         CAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGC                                                                                                                              <  SRR19973061.2682299/150‑1 (MQ=60)
                          AACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCT                                                                                                                             >  SRR19973061.808855/1‑150 (MQ=60)
                           ACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTT                                                                                                                            <  SRR19973061.2431630/150‑1 (MQ=60)
                             CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                          <  SRR19973061.3411131/150‑1 (MQ=60)
                             CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                          >  SRR19973061.3266753/1‑150 (MQ=60)
                              ACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAG                                                                                                                         >  SRR19973061.2379843/1‑150 (MQ=60)
                              ACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAG                                                                                                                         <  SRR19973061.3597574/150‑1 (MQ=60)
                                AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                       <  SRR19973061.137083/150‑1 (MQ=60)
                                AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                       <  SRR19973061.3547609/150‑1 (MQ=60)
                                AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                       <  SRR19973061.2951999/150‑1 (MQ=60)
                                 ACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTG                                                                                                                      <  SRR19973061.3031867/150‑1 (MQ=60)
                                   GCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAA                                                                                                                    <  SRR19973061.1292696/150‑1 (MQ=60)
                                        GGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGT                                                                                                               >  SRR19973061.1153035/1‑150 (MQ=60)
                                           GAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTA                                                                                                            >  SRR19973061.1701646/1‑150 (MQ=60)
                                           GAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTA                                                                                                            <  SRR19973061.1463488/150‑1 (MQ=60)
                                           GAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTA                                                                                                            >  SRR19973061.1965080/1‑150 (MQ=60)
                                            AGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAA                                                                                                           <  SRR19973061.1148304/150‑1 (MQ=60)
                                              CGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATC                                                                                                         <  SRR19973061.511122/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGGTAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973061.152863/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973061.152859/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        >  SRR19973061.573539/1‑150 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973061.1378717/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973061.1380653/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973061.152844/150‑1 (MQ=60)
                                                CCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGAGAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAA                                                                                                       <  SRR19973061.1630550/150‑1 (MQ=60)
                                                 CAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAT                                                                                                      >  SRR19973061.774679/1‑150 (MQ=60)
                                                  AGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATA                                                                                                     <  SRR19973061.3206413/150‑1 (MQ=60)
                                                      TGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAA                                                                                                 >  SRR19973061.2592932/1‑150 (MQ=60)
                                                      TGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAA                                                                                                 <  SRR19973061.2149128/150‑1 (MQ=60)
                                                      TGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAA                                                                                                 >  SRR19973061.3111654/1‑150 (MQ=60)
                                                       GGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAT                                                                                                <  SRR19973061.1923363/150‑1 (MQ=60)
                                                       GGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAT                                                                                                <  SRR19973061.3574847/150‑1 (MQ=60)
                                                           CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                            <  SRR19973061.2807897/150‑1 (MQ=60)
                                                           CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                            <  SRR19973061.3653894/150‑1 (MQ=60)
                                                           CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                            <  SRR19973061.3655654/150‑1 (MQ=60)
                                                           CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                            <  SRR19973061.1997496/150‑1 (MQ=60)
                                                              GCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTA                                                                                         <  SRR19973061.310544/150‑1 (MQ=60)
                                                              GCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTA                                                                                         >  SRR19973061.709240/1‑150 (MQ=60)
                                                                  AATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATT                                                                                     <  SRR19973061.391265/150‑1 (MQ=60)
                                                                   ATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTG                                                                                    <  SRR19973061.1158984/150‑1 (MQ=60)
                                                                    TCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGG                                                                                   <  SRR19973061.1726726/150‑1 (MQ=60)
                                                                      ACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCT                                                                                 <  SRR19973061.451813/150‑1 (MQ=60)
                                                                        CGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGT                                                                               <  SRR19973061.1935208/150‑1 (MQ=60)
                                                                        CGGCAAAGACAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGT                                                                               <  SRR19973061.19290/150‑1 (MQ=60)
                                                                         GGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTA                                                                              <  SRR19973061.195455/150‑1 (MQ=60)
                                                                         GGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTA                                                                              <  SRR19973061.3692098/150‑1 (MQ=60)
                                                                             AAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTT                                                                          <  SRR19973061.1049357/150‑1 (MQ=60)
                                                                             AAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTT                                                                          <  SRR19973061.2956453/150‑1 (MQ=60)
                                                                              AGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTG                                                                         >  SRR19973061.3195850/1‑150 (MQ=60)
                                                                              AGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTG                                                                         <  SRR19973061.2851196/150‑1 (MQ=60)
                                                                                       GGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGC                                                                >  SRR19973061.1618149/1‑150 (MQ=60)
                                                                                          ACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCA                                                             >  SRR19973061.373938/1‑150 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            <  SRR19973061.3180/150‑1 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            <  SRR19973061.3633530/150‑1 (MQ=60)
                                                                                               TTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATG                                                        >  SRR19973061.911790/1‑150 (MQ=60)
                                                                                                     TGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCG                                                  >  SRR19973061.806032/1‑150 (MQ=60)
                                                                                                     TGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCG                                                  >  SRR19973061.3237661/1‑150 (MQ=60)
                                                                                                       ATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTG                                                <  SRR19973061.1162749/150‑1 (MQ=60)
                                                                                                          ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             <  SRR19973061.2785927/150‑1 (MQ=60)
                                                                                                          ATCCTACGCCTGCTGGTGGAAATAACAACAAGCAGCCTCAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             <  SRR19973061.1511925/150‑1 (MQ=60)
                                                                                                           TCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTA                                            <  SRR19973061.3310778/150‑1 (MQ=60)
                                                                                                            CCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAT                                           <  SRR19973061.2404021/150‑1 (MQ=60)
                                                                                                            CCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAT                                           >  SRR19973061.3180912/1‑150 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                          <  SRR19973061.2021296/150‑1 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGA                                      >  SRR19973061.3290600/1‑150 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     >  SRR19973061.2833675/1‑150 (MQ=60)
                                                                                                                   CTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATT                                    <  SRR19973061.1531078/150‑1 (MQ=60)
                                                                                                                    TGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTG                                   <  SRR19973061.1447830/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                                <  SRR19973061.3648764/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                                <  SRR19973061.3648787/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                                <  SRR19973061.3648816/150‑1 (MQ=60)
                                                                                                                        GGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGA                               <  SRR19973061.2998617/150‑1 (MQ=60)
                                                                                                                          TGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGA                             >  SRR19973061.775831/1‑150 (MQ=60)
                                                                                                                          TGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGA                             >  SRR19973061.583276/1‑150 (MQ=60)
                                                                                                                           GGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAA                            <  SRR19973061.527235/150‑1 (MQ=60)
                                                                                                                           GGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAA                            >  SRR19973061.477218/1‑150 (MQ=60)
                                                                                                                            GAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAAT                           <  SRR19973061.1032182/150‑1 (MQ=60)
                                                                                                                            GAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAAT                           <  SRR19973061.249723/150‑1 (MQ=60)
                                                                                                                             AAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATA                          <  SRR19973061.1878345/150‑1 (MQ=60)
                                                                                                                             AAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATA                          >  SRR19973061.2108309/1‑150 (MQ=60)
                                                                                                                              AATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAA                         <  SRR19973061.500953/150‑1 (MQ=60)
                                                                                                                              AATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAA                         <  SRR19973061.500537/150‑1 (MQ=60)
                                                                                                                               ATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAtcgga                        >  SRR19973061.1106052/1‑145 (MQ=60)
                                                                                                                               ATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAA                        >  SRR19973061.1506271/1‑150 (MQ=60)
                                                                                                                               ATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAA                        >  SRR19973061.393953/1‑150 (MQ=60)
                                                                                                                                TAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAA                       <  SRR19973061.3064455/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973061.3341173/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973061.3341253/150‑1 (MQ=60)
                                                                                                                                  ACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACC                     <  SRR19973061.110557/150‑1 (MQ=60)
                                                                                                                                  ACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACC                     >  SRR19973061.1655446/1‑150 (MQ=60)
                                                                                                                                  ACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACC                     <  SRR19973061.1940934/150‑1 (MQ=60)
                                                                                                                                    AACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGC                   >  SRR19973061.2592369/1‑150 (MQ=60)
                                                                                                                                       AAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTC                <  SRR19973061.180900/150‑1 (MQ=60)
                                                                                                                                        AGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCC               >  SRR19973061.2837366/1‑150 (MQ=60)
                                                                                                                                         GCAGCCAAAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCA              <  SRR19973061.2828227/150‑1 (MQ=60)
                                                                                                                                          CAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCTGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCAT             <  SRR19973061.365915/150‑1 (MQ=60)
                                                                                                                                          CAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTTGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCAT             <  SRR19973061.366034/150‑1 (MQ=60)
                                                                                                                                          CAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCAT             >  SRR19973061.428621/1‑150 (MQ=60)
                                                                                                                                              CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         >  SRR19973061.433050/1‑150 (MQ=60)
                                                                                                                                              CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         >  SRR19973061.103010/1‑150 (MQ=60)
                                                                                                                                                CAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAATGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT       <  SRR19973061.1921106/150‑1 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      <  SRR19973061.878741/150‑1 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      <  SRR19973061.879602/150‑1 (MQ=60)
                                                                                                                                                  ATTCCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAC     <  SRR19973061.537166/150‑1 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  SRR19973061.3047811/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494871‑495169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑