Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,020 T→A 100% E137V (GAA→GTA)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,0200TA97.2% 796.5 / 8.6 249E137V (GAA→GTA) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base A (115/127);  total (115/134)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA  >  CP009273/494875‑495162
                                                                                                                                                 |                                                                                                                                              
gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTAcc                                                                                                                                              >  1:2467947/1‑148 (MQ=255)
gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTAcc                                                                                                                                              >  1:3267411/1‑148 (MQ=255)
  aaTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACACATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctc                                                                                                                                            <  2:881215/148‑1 (MQ=255)
   aTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctct                                                                                                                                           <  2:3492298/148‑1 (MQ=255)
   aTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctct                                                                                                                                           <  2:2354521/148‑1 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                          <  2:1013926/148‑1 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                          >  2:339900/1‑148 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                          <  1:2922247/148‑1 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                          <  2:2713001/148‑1 (MQ=255)
    tCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTa                                                                                                                                          >  1:373974/1‑148 (MQ=255)
     cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTAt                                                                                                                                         <  1:1402002/148‑1 (MQ=255)
     cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTAt                                                                                                                                         <  2:1227323/148‑1 (MQ=255)
         cTGTTTATCATGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                     >  2:1586841/1‑148 (MQ=255)
         cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                     <  1:1490206/148‑1 (MQ=255)
         cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                     >  1:895407/1‑148 (MQ=255)
          tGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCtt                                                                                                                                    >  2:615330/1‑148 (MQ=255)
           gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTg                                                                                                                                   >  1:1856288/1‑148 (MQ=255)
                 cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaa                                                                                                                             >  1:3333542/1‑148 (MQ=255)
                             aCGCACTGGCGAGCGCCAGCATGGCACCTGCGTAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                 <  1:358195/148‑1 (MQ=255)
                             aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                 <  1:1098959/148‑1 (MQ=255)
                             aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                 <  1:126464/148‑1 (MQ=255)
                             aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                 <  2:892753/148‑1 (MQ=255)
                              cGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGt                                                                                                                <  1:1852385/148‑1 (MQ=255)
                              cGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGt                                                                                                                >  2:1989581/1‑148 (MQ=255)
                                cACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGa                                                                                                              >  2:300495/1‑148 (MQ=255)
                                cACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGa                                                                                                              >  2:3239401/1‑148 (MQ=255)
                                    ggCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAgg                                                                                                          <  2:2274312/148‑1 (MQ=255)
                                     gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAggg                                                                                                         <  1:494782/148‑1 (MQ=255)
                                        aGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgtgt                                                                                                      <  1:643311/148‑1 (MQ=255)
                                         gcgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTa                                                                                                     <  2:277190/148‑1 (MQ=255)
                                           gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAAt                                                                                                   <  2:2416081/148‑1 (MQ=255)
                                           gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAAt                                                                                                   <  1:2874891/148‑1 (MQ=255)
                                           gcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAAt                                                                                                   <  2:3112829/148‑1 (MQ=255)
                                               gCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAt                                                                                               <  1:771851/148‑1 (MQ=255)
                                               gCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAt                                                                                               <  1:2559685/148‑1 (MQ=255)
                                               gCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAt                                                                                               >  1:1162168/1‑148 (MQ=255)
                                                  tGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATAcc                                                                                            >  1:1480523/1‑148 (MQ=255)
                                                   ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                           <  2:814429/148‑1 (MQ=255)
                                                   ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                           >  2:728417/1‑148 (MQ=255)
                                                    gCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCaa                                                                                          >  2:2807009/1‑148 (MQ=255)
                                                           cGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGt                                                                                   >  2:2548659/1‑148 (MQ=255)
                                                              aaTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACa                                                                                >  1:493276/1‑148 (MQ=255)
                                                              aaTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATA‑CAATCACCGTACAt                                                                               <  2:3327097/148‑1 (MQ=255)
                                                               aTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAt                                                                               <  1:3433315/148‑1 (MQ=255)
                                                                tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                              >  2:3322676/1‑148 (MQ=255)
                                                                tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                              >  1:2711661/1‑148 (MQ=255)
                                                                 cACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTg                                                                             >  1:2423730/1‑148 (MQ=255)
                                                                 cACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTg                                                                             >  2:1968390/1‑148 (MQ=255)
                                                                 cACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTg                                                                             >  2:1479231/1‑148 (MQ=255)
                                                                  aCCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTgg                                                                            <  1:77401/148‑1 (MQ=255)
                                                                   ccGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGc                                                                           <  1:340959/148‑1 (MQ=255)
                                                                     ggCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTg                                                                         <  2:1898255/148‑1 (MQ=255)
                                                                     ggCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTg                                                                         <  2:1704987/148‑1 (MQ=255)
                                                                         aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGc                                                                     <  1:615330/148‑1 (MQ=255)
                                                                          aGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCt                                                                    >  1:2232907/1‑148 (MQ=255)
                                                                               aTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCca                                                               >  2:2073213/1‑148 (MQ=255)
                                                                               aTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCca                                                               >  1:1367434/1‑148 (MQ=255)
                                                                                    ggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTTCATTGGCTGTAGCTTGCCAGCAgg                                                          >  2:3093263/1‑148 (MQ=255)
                                                                                     gACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGgc                                                         >  1:877769/1‑148 (MQ=255)
                                                                                         tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                     >  2:1169030/1‑148 (MQ=255)
                                                                                          atTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                    <  1:2929655/148‑1 (MQ=255)
                                                                                          atTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                    <  2:2144573/148‑1 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                   <  1:1114501/148‑1 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                   >  1:1631440/1‑148 (MQ=255)
                                                                                             gatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatgatg                                                 <  1:3191868/148‑1 (MQ=255)
                                                                                                ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                              <  1:2356730/148‑1 (MQ=255)
                                                                                                    tAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGt                                          <  1:399354/148‑1 (MQ=255)
                                                                                                    tAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGt                                          <  2:3267411/148‑1 (MQ=255)
                                                                                                    tAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGt                                          >  1:2516929/1‑148 (MQ=255)
                                                                                                     aaTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                         >  2:2341876/1‑148 (MQ=255)
                                                                                                     aaTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                         >  2:1433461/1‑148 (MQ=255)
                                                                                                      aTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTgg                                        >  2:3556385/1‑148 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                      >  2:95857/1‑148 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                      >  2:1743505/1‑148 (MQ=255)
                                                                                                         ctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTa                                     <  1:2978287/148‑1 (MQ=255)
                                                                                                          tccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATAACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAt                                    <  1:2183186/148‑1 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                <  2:947261/148‑1 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                <  1:2211261/148‑1 (MQ=255)
                                                                                                               ctgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                               >  1:1662725/1‑148 (MQ=255)
                                                                                                               ctgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                               >  1:1918444/1‑148 (MQ=255)
                                                                                                               ctgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                               <  1:2762359/148‑1 (MQ=255)
                                                                                                               ctgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACAGAAAATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCTTGGGTATCgaga                               >  1:1918300/1‑148 (MQ=255)
                                                                                                                tgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                              >  1:1995042/1‑148 (MQ=255)
                                                                                                                 gctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAtt                             >  2:1832807/1‑148 (MQ=255)
                                                                                                                    ggtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGcc                          >  2:1958919/1‑148 (MQ=255)
                                                                                                                     gtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                         >  1:3508465/1‑148 (MQ=255)
                                                                                                                     gtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                         >  2:3527559/1‑148 (MQ=255)
                                                                                                                      tggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCga                        <  1:1284059/148‑1 (MQ=255)
                                                                                                                        gAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgaga                      >  2:25617/1‑148 (MQ=255)
                                                                                                                        gAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgaga                      <  2:167614/148‑1 (MQ=255)
                                                                                                                        gAAAGAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgaga                      <  2:2450794/148‑1 (MQ=255)
                                                                                                                           aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                   <  2:3140708/148‑1 (MQ=255)
                                                                                                                           aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                   >  2:1077576/1‑148 (MQ=255)
                                                                                                                             aacaacAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                 <  1:785952/148‑1 (MQ=255)
                                                                                                                             aacaacAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                 <  1:787712/148‑1 (MQ=255)
                                                                                                                                   aaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGcc           <  2:830594/148‑1 (MQ=255)
                                                                                                                                       agcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCa       <  2:2932111/148‑1 (MQ=255)
                                                                                                                                         ccACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATg     <  1:2076392/148‑1 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa  >  1:272569/1‑148 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa  >  2:1253021/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA  >  CP009273/494875‑495162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494871‑495169
                                                                                                                                                     |                                                                                                                                                     
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAATGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTA                                                                                                                                                       <  SRR19973066.1304529/150‑1 (MQ=60)
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTA                                                                                                                                                       >  SRR19973066.854701/1‑150 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                     >  SRR19973066.2484775/1‑150 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                     <  SRR19973066.2572796/150‑1 (MQ=60)
  CCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACC                                                                                                                                                     >  SRR19973066.3288875/1‑150 (MQ=60)
      AATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTA                                                                                                                                                 >  SRR19973066.376913/1‑150 (MQ=60)
        TCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATC                                                                                                                                               <  SRR19973066.2944497/150‑1 (MQ=60)
        TCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATC                                                                                                                                               <  SRR19973066.2941726/150‑1 (MQ=60)
         CGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCG                                                                                                                                              <  SRR19973066.1412098/150‑1 (MQ=60)
           ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCT                                                                                                                                            >  SRR19973066.901740/1‑150 (MQ=60)
           ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCT                                                                                                                                            >  SRR19973066.870441/1‑150 (MQ=60)
            TCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTT                                                                                                                                           <  SRR19973066.1173382/150‑1 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          <  SRR19973066.1500289/150‑1 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          >  SRR19973066.1869567/1‑150 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          >  SRR19973066.169126/1‑150 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          <  SRR19973066.1500638/150‑1 (MQ=60)
             CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTG                                                                                                                                          <  SRR19973066.2905553/150‑1 (MQ=60)
              TGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGCACATATTGATTTGATAATCCTCCGCCTGCTGGTGTAAATAACAACAAGAAGCCACAATTACCTCTATCGCTTGC                                                                                                                                         >  SRR19973066.1649892/1‑150 (MQ=60)
               GTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCG                                                                                                                                        >  SRR19973066.1232786/1‑150 (MQ=60)
               GTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCG                                                                                                                                        >  SRR19973066.1636755/1‑150 (MQ=60)
                   ATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAA                                                                                                                                    >  SRR19973066.3355711/1‑150 (MQ=60)
                   ATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAA                                                                                                                                    >  SRR19973066.3070267/1‑150 (MQ=60)
                   ATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAA                                                                                                                                    >  SRR19973066.1283122/1‑150 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACCTATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                                  >  SRR19973066.900875/1‑150 (MQ=60)
                     CACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAAC                                                                                                                                  >  SRR19973066.1372576/1‑150 (MQ=60)
                            CCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTC                                                                                                                           >  SRR19973066.238269/1‑150 (MQ=60)
                               CAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGG                                                                                                                        <  SRR19973066.259837/150‑1 (MQ=60)
                                 ACGCACTGGCGAGCGCCAGCATGGCACCTGCGTAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTG                                                                                                                      <  SRR19973066.360989/150‑1 (MQ=60)
                                 ACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTG                                                                                                                      <  SRR19973066.1106947/150‑1 (MQ=60)
                                 ACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTG                                                                                                                      <  SRR19973066.1942380/150‑1 (MQ=60)
                                 ACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTG                                                                                                                      <  SRR19973066.127719/150‑1 (MQ=60)
                                  CGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGA                                                                                                                     <  SRR19973066.1865642/150‑1 (MQ=60)
                                     ACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAG                                                                                                                  <  SRR19973066.3332445/150‑1 (MQ=60)
                                         GCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTG                                                                                                              <  SRR19973066.498468/150‑1 (MQ=60)
                                         GCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTG                                                                                                              <  SRR19973066.498473/150‑1 (MQ=60)
                                            AGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAA                                                                                                           <  SRR19973066.288344/150‑1 (MQ=60)
                                            AGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAA                                                                                                           <  SRR19973066.647968/150‑1 (MQ=60)
                                              CGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATC                                                                                                         <  SRR19973066.1344761/150‑1 (MQ=60)
                                              CGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATC                                                                                                         <  SRR19973066.3574623/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973066.2381920/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973066.2894509/150‑1 (MQ=60)
                                               GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        <  SRR19973066.2894158/150‑1 (MQ=60)
                                                 CAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAT                                                                                                      >  SRR19973066.1170412/1‑150 (MQ=60)
                                                 CAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAT                                                                                                      <  SRR19973066.2245130/150‑1 (MQ=60)
                                                  AGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATA                                                                                                     >  SRR19973066.648147/1‑150 (MQ=60)
                                                   GCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATAC                                                                                                    <  SRR19973066.2577059/150‑1 (MQ=60)
                                                   GCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATAC                                                                                                    <  SRR19973066.777565/150‑1 (MQ=60)
                                                    CATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACC                                                                                                   >  SRR19973066.1490378/1‑150 (MQ=60)
                                                    CATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACC                                                                                                   >  SRR19973066.1490405/1‑150 (MQ=60)
                                                    CATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACC                                                                                                   >  SRR19973066.1490928/1‑150 (MQ=60)
                                                     ATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCA                                                                                                  <  SRR19973066.2096616/150‑1 (MQ=60)
                                                     ATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCA                                                                                                  >  SRR19973066.1885070/1‑150 (MQ=60)
                                                       GGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAT                                                                                                <  SRR19973066.1717394/150‑1 (MQ=60)
                                                       GGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAT                                                                                                <  SRR19973066.3490471/150‑1 (MQ=60)
                                                                GGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACA                                                                                       >  SRR19973066.1933253/1‑150 (MQ=60)
                                                                GGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACA                                                                                       >  SRR19973066.117342/1‑150 (MQ=60)
                                                                GGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACA                                                                                       >  SRR19973066.496963/1‑150 (MQ=60)
                                                                  AATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATT                                                                                     >  SRR19973066.2729872/1‑150 (MQ=60)
                                                                  AATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATT                                                                                     >  SRR19973066.2729870/1‑150 (MQ=60)
                                                                   ATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTG                                                                                    >  SRR19973066.2440188/1‑150 (MQ=60)
                                                                   ATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTG                                                                                    <  SRR19973066.3456287/150‑1 (MQ=60)
                                                                   ATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTG                                                                                    >  SRR19973066.793785/1‑150 (MQ=60)
                                                                   ATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATNG                                                                                    <  SRR19973066.2169192/150‑1 (MQ=60)
                                                                      ACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCT                                                                                 <  SRR19973066.78307/150‑1 (MQ=60)
                                                                      ACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCT                                                                                 <  SRR19973066.78105/150‑1 (MQ=60)
                                                                       CCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTG                                                                                <  SRR19973066.343588/150‑1 (MQ=60)
                                                                       CCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTG                                                                                <  SRR19973066.343613/150‑1 (MQ=60)
                                                                          GCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAG                                                                             <  SRR19973066.3368139/150‑1 (MQ=60)
                                                                           CAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGC                                                                            >  SRR19973066.2247404/1‑150 (MQ=60)
                                                                            AAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCT                                                                           >  SRR19973066.2248317/1‑150 (MQ=60)
                                                                             AAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTT                                                                          <  SRR19973066.619712/150‑1 (MQ=60)
                                                                             AAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTT                                                                          <  SRR19973066.619650/150‑1 (MQ=60)
                                                                                 CAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCA                                                                      >  SRR19973066.1377332/1‑150 (MQ=60)
                                                                                 CAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCA                                                                      >  SRR19973066.1377325/1‑150 (MQ=60)
                                                                                       GGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGC                                                                >  SRR19973066.884028/1‑150 (MQ=60)
                                                                                           CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            >  SRR19973066.2773746/1‑150 (MQ=60)
                                                                                            ATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATG                                                           >  SRR19973066.3258115/1‑150 (MQ=60)
                                                                                             TATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGA                                                          >  SRR19973066.1643295/1‑150 (MQ=60)
                                                                                              ATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGAT                                                         <  SRR19973066.2949161/150‑1 (MQ=60)
                                                                                              ATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGAT                                                         >  SRR19973066.418364/1‑150 (MQ=60)
                                                                                              ATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGAT                                                         >  SRR19973066.418402/1‑150 (MQ=60)
                                                                                               TTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATG                                                        <  SRR19973066.1122536/150‑1 (MQ=60)
                                                                                               TTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATG                                                        <  SRR19973066.1122547/150‑1 (MQ=60)
                                                                                                 GATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCG                                                      <  SRR19973066.3213077/150‑1 (MQ=60)
                                                                                                    TTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATC                                                   <  SRR19973066.2372721/150‑1 (MQ=60)
                                                                                                     TGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCG                                                  >  SRR19973066.3268988/1‑150 (MQ=60)
                                                                                                      GATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGT                                                 >  SRR19973066.2534195/1‑150 (MQ=60)
                                                                                                      GATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGT                                                 >  SRR19973066.2534050/1‑150 (MQ=60)
                                                                                                        TAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGG                                               <  SRR19973066.402496/150‑1 (MQ=60)
                                                                                                        TAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGG                                               <  SRR19973066.402491/150‑1 (MQ=60)
                                                                                                          ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             >  SRR19973066.336727/1‑150 (MQ=60)
                                                                                                            CCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTAT                                           <  SRR19973066.270712/150‑1 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                          <  SRR19973066.2997892/150‑1 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                          <  SRR19973066.2997965/150‑1 (MQ=60)
                                                                                                             CTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                          >  SRR19973066.561293/1‑150 (MQ=60)
                                                                                                              TCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCG                                         <  SRR19973066.550651/150‑1 (MQ=60)
                                                                                                              TCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATAACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCG                                         <  SRR19973066.2198339/150‑1 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGA                                      >  SRR19973066.1932072/1‑150 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGA                                      >  SRR19973066.1932095/1‑150 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGA                                      >  SRR19973066.1674837/1‑150 (MQ=60)
                                                                                                                 GCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACAGAAAATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCTTGGGTATCGAGA                                      >  SRR19973066.1931949/1‑150 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     >  SRR19973066.2512964/1‑150 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     >  SRR19973066.2009075/1‑150 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     <  SRR19973066.2226578/150‑1 (MQ=60)
                                                                                                                  CCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     <  SRR19973066.2226522/150‑1 (MQ=60)
                                                                                                                   CTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATT                                    <  SRR19973066.684049/150‑1 (MQ=60)
                                                                                                                   CTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATT                                    <  SRR19973066.2780956/150‑1 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                                >  SRR19973066.3531843/1‑150 (MQ=60)
                                                                                                                       TGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCG                                >  SRR19973066.2272246/1‑150 (MQ=60)
                                                                                                                          TGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGA                             <  SRR19973066.1293332/150‑1 (MQ=60)
                                                                                                                           GGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAA                            >  SRR19973066.1887822/1‑150 (MQ=60)
                                                                                                                               ATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAA                        >  SRR19973066.2972232/1‑150 (MQ=60)
                                                                                                                                TAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAA                       <  SRR19973066.975516/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.793517/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.792421/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.3477304/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      >  SRR19973066.2484243/1‑150 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.791747/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.791719/150‑1 (MQ=60)
                                                                                                                                 AACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAC                      <  SRR19973066.791701/150‑1 (MQ=60)
                                                                                                                                   CAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                    <  SRR19973066.1503388/150‑1 (MQ=60)
                                                                                                                                      CAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 <  SRR19973066.376185/150‑1 (MQ=60)
                                                                                                                                      CAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 <  SRR19973066.373979/150‑1 (MQ=60)
                                                                                                                                           AGCCACAATTTCCTCTATCGCTTGCGTAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATTTTGCGATCGTGGGTATCGAGTTTGCCGAGAATAAAACCGCCTCCATG            <  SRR19973066.1867343/150‑1 (MQ=60)
                                                                                                                                           AGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATG            >  SRR19973066.2517525/1‑150 (MQ=60)
                                                                                                                                             CCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCA          <  SRR19973066.2090743/150‑1 (MQ=60)
                                                                                                                                              CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         >  SRR19973066.274581/1‑150 (MQ=60)
                                                                                                                                              CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         >  SRR19973066.2090301/1‑150 (MQ=60)
                                                                                                                                              CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         >  SRR19973066.275356/1‑150 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      >  SRR19973066.490101/1‑150 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      >  SRR19973066.2018964/1‑150 (MQ=60)
                                                                                                                                                 AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      >  SRR19973066.2791168/1‑150 (MQ=60)
                                                                                                                                                    TACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAA   >  SRR19973066.1725686/1‑150 (MQ=60)
                                                                                                                                                    TACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAA   >  SRR19973066.2327095/1‑150 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGTAAAACGCGCTTCAGGTGAAAGGGTTTAATCAATACCAATCCCCTTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCTATCGTGGTTATCGAGTTTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  <  SRR19973066.1541575/150‑1 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTTGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  SRR19973066.3425914/1‑150 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTTGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  SRR19973066.774427/1‑150 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTTGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  SRR19973066.3425924/1‑150 (MQ=60)
                                                                                                                                                     ACCTCTATCGCTTGCGCAAAACGCGCTTCAGGTGAACGGGTGTAATAAATACCAATCCCCGTACATTGGCTTTAGGTTGCCAGCAGGCGCTTGATGCGATCGTGGTTATCGAGTTGGCCTACAATAAAACCGCCTCCATGCAAATAAAAT  <  SRR19973066.1541571/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494871‑495169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑