Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,020 T→A 100% E137V (GAA→GTA)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,0200TA99.1% 702.7 / ‑2.7 222E137V (GAA→GTA) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (99/120);  total (99/123)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT  >  CP009273/494876‑495164
                                                                                                                                                 |                                                                                                                                                
cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACct                                                                                                                                               >  2:1725592/1‑148 (MQ=255)
cAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACct                                                                                                                                               <  2:2066176/148‑1 (MQ=255)
  aTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACctct                                                                                                                                             >  1:429523/1‑148 (MQ=255)
  aTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACctct                                                                                                                                             <  2:2373490/148‑1 (MQ=255)
    cGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTAt                                                                                                                                           <  1:2674474/148‑1 (MQ=255)
      aTCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCg                                                                                                                                         >  2:3046018/1‑148 (MQ=255)
      aTCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCg                                                                                                                                         >  2:67985/1‑148 (MQ=255)
       tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGc                                                                                                                                        <  2:2256447/148‑1 (MQ=255)
        cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCt                                                                                                                                       >  1:1389417/1‑148 (MQ=255)
        cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCt                                                                                                                                       >  1:890956/1‑148 (MQ=255)
           tttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGc                                                                                                                                    >  2:1647715/1‑148 (MQ=255)
                cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGaaa                                                                                                                               <  2:1119191/148‑1 (MQ=255)
                 aCGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGaaaa                                                                                                                              >  1:2198285/1‑148 (MQ=255)
                    cAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAAcgc                                                                                                                           >  2:3316424/1‑148 (MQ=255)
                      aCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACgcgc                                                                                                                         <  2:2744966/148‑1 (MQ=255)
                      aCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACgcgc                                                                                                                         <  2:2018608/148‑1 (MQ=255)
                       ccACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCt                                                                                                                        <  1:2815075/148‑1 (MQ=255)
                         acaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTc                                                                                                                      <  2:2940436/148‑1 (MQ=255)
                          caACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                     <  1:257856/148‑1 (MQ=255)
                          caACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                     >  2:893487/1‑148 (MQ=255)
                          caACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCa                                                                                                                     >  1:1787274/1‑148 (MQ=255)
                           aaCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAg                                                                                                                    >  2:2913921/1‑148 (MQ=255)
                            aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                   <  2:2619384/148‑1 (MQ=255)
                            aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAgg                                                                                                                   <  2:976812/148‑1 (MQ=255)
                             cGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGt                                                                                                                  <  2:1000505/148‑1 (MQ=255)
                                    gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAggg                                                                                                           >  1:2790998/1‑148 (MQ=255)
                                      gAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgtg                                                                                                         <  1:3467580/148‑1 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      <  2:2444927/148‑1 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      >  2:1970785/1‑148 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      <  2:1234437/148‑1 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      <  2:3212117/148‑1 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      <  2:3362417/148‑1 (MQ=255)
                                         cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                      <  2:3508882/148‑1 (MQ=255)
                                            cAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                   >  1:2650246/1‑148 (MQ=255)
                                              gCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAt                                                                                                 <  2:2828414/148‑1 (MQ=255)
                                                    cACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAAt                                                                                           <  1:2429695/148‑1 (MQ=255)
                                                     aCCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAG‑CACACCGTACCTCTATCTATTTCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATc                                                                                          <  1:3447689/148‑1 (MQ=255)
                                                           ggAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTa                                                                                    <  2:455672/148‑1 (MQ=255)
                                                             aaTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACa                                                                                  <  1:2968357/148‑1 (MQ=255)
                                                              aTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAt                                                                                 >  1:655507/1‑148 (MQ=255)
                                                               tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                                >  2:406780/1‑148 (MQ=255)
                                                               tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                                <  1:448396/148‑1 (MQ=255)
                                                               tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCAACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                                >  2:2600377/1‑148 (MQ=255)
                                                                 aCCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTgg                                                                              >  1:1069920/1‑148 (MQ=255)
                                                                     gCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGt                                                                          <  2:710190/148‑1 (MQ=255)
                                                                      cAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTa                                                                         >  2:1780629/1‑148 (MQ=255)
                                                                        aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGc                                                                       <  2:2786897/148‑1 (MQ=255)
                                                                        aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGc                                                                       <  1:1006089/148‑1 (MQ=255)
                                                                            cAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGc                                                                   <  2:2068145/148‑1 (MQ=255)
                                                                            cAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGc                                                                   <  2:1230419/148‑1 (MQ=255)
                                                                               tGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcag                                                                >  1:1189999/1‑148 (MQ=255)
                                                                                gCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcagc                                                               >  1:1538896/1‑148 (MQ=255)
                                                                                 cGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcagca                                                              <  1:3218212/148‑1 (MQ=255)
                                                                                 cGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcagca                                                              <  1:3221427/148‑1 (MQ=255)
                                                                                   ggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAgg                                                            >  1:1233380/1‑148 (MQ=255)
                                                                                      cATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGgcgc                                                         <  1:339330/148‑1 (MQ=255)
                                                                                       atatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACCAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCa                                                        <  1:1588537/148‑1 (MQ=255)
                                                                                       atatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCa                                                        >  2:2760244/1‑148 (MQ=255)
                                                                                        tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                       >  1:1025546/1‑148 (MQ=255)
                                                                                        tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                       >  1:1461802/1‑148 (MQ=255)
                                                                                         atggatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                      <  2:1952080/145‑1 (MQ=255)
                                                                                            gatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatgatg                                                   >  2:2855412/1‑148 (MQ=255)
                                                                                               ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                >  1:1743763/1‑148 (MQ=255)
                                                                                               ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                >  1:485284/1‑148 (MQ=255)
                                                                                               ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                <  1:2610747/148‑1 (MQ=255)
                                                                                               ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                >  1:933554/1‑148 (MQ=255)
                                                                                                 gatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATc                                              <  2:1389417/148‑1 (MQ=255)
                                                                                                    aaTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                           >  2:3337543/1‑148 (MQ=255)
                                                                                                    aaTCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                           <  1:2465046/148‑1 (MQ=255)
                                                                                                    aaTCCTCCGCCTGCTGGTGGAAATAACAACAAGAAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTg                                           >  2:2551631/1‑148 (MQ=255)
                                                                                                      tcctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTggg                                         <  2:2666733/148‑1 (MQ=255)
                                                                                                          ccGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCGTGGGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGtctc                                     >  2:1035580/1‑148 (MQ=255)
                                                                                                           cGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCg                                    <  1:1311636/148‑1 (MQ=255)
                                                                                                              ctgctgGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                                 <  1:1345977/148‑1 (MQ=255)
                                                                                                              ctgctgGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                                 <  2:887833/148‑1 (MQ=255)
                                                                                                               tgctgGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                                >  1:2726042/1‑148 (MQ=255)
                                                                                                                 ctgGTGGAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTg                              >  1:1997364/1‑148 (MQ=255)
                                                                                                                   ggtggAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGcc                            >  1:3064485/1‑148 (MQ=255)
                                                                                                                    gtggAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCg                           <  1:69135/148‑1 (MQ=255)
                                                                                                                     tggAAATAACAACAAGCAGCCACA‑ATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCga                          <  1:1286479/148‑1 (MQ=255)
                                                                                                                     tggAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCga                          >  2:925070/1‑148 (MQ=255)
                                                                                                                      ggAAATAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCgag                         <  2:2777317/148‑1 (MQ=255)
                                                                                                                          aTAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                     <  1:1970785/148‑1 (MQ=255)
                                                                                                                           tAACAACAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaa                    >  1:608278/1‑148 (MQ=255)
                                                                                                                            aacaacAAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                   <  2:2160534/148‑1 (MQ=255)
                                                                                                                             acaacaAGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaaa                  <  2:2341056/148‑1 (MQ=255)
                                                                                                                              caacaaGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                 <  1:392787/148‑1 (MQ=255)
                                                                                                                              caacaaGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                 <  1:392951/148‑1 (MQ=255)
                                                                                                                                 caaGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGc              <  1:3316424/148‑1 (MQ=255)
                                                                                                                                 caaGCAGCCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGc              >  1:791086/1‑148 (MQ=255)
                                                                                                                                   agcagcCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAATGGGTGTAATAAATACCAATCCCCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGTTATTGCCGAGAATAAAACCGCCt            <  2:1217867/148‑1 (MQ=255)
                                                                                                                                   agcagcCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCt            >  1:779579/1‑148 (MQ=255)
                                                                                                                                     cagcCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTcc          >  2:133480/1‑148 (MQ=255)
                                                                                                                                       gcCACA‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCAt        >  2:1578839/1‑148 (MQ=255)
                                                                                                                                         caca‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCTGGCGCTTGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGc      <  1:441659/148‑1 (MQ=255)
                                                                                                                                         caca‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGc      >  1:3146885/1‑148 (MQ=255)
                                                                                                                                           ca‑ATTCCCTCTCTGGCTTGCGGAAAACGCGCTTCTGTTGAAAGGTTGTAATCAATACCATTCACGGTACTTGGGCTGTAGGTTGCCAGCCGGCGCATTATGCGTTCGTGGGTATCTAGTTTGCCGAGAATAAAACCGCCTCCATGCaa    <  1:2138176/148‑1 (MQ=255)
                                                                                                                                           ca‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa    <  1:3046018/148‑1 (MQ=255)
                                                                                                                                           ca‑ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa    <  1:2905232/148‑1 (MQ=255)
                                                                                                                                              aTTACCTCTATCTGTTGCTGAAAACGCGCTTCTGGTGAATGGGTGTAATCAATACAAATCACCGTAAATTGGCTTTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGATAATAAAACCGCCTCCATGCaaat  <  1:1932238/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACA‑ATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT  >  CP009273/494876‑495164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494874‑495169
                                                                                                                                                   |                                                                                                                                                     
CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCT                                                                                                                                                    >  SRR19973065.339146/1‑150 (MQ=60)
CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCT                                                                                                                                                    >  SRR19973065.372454/1‑150 (MQ=60)
  CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCT                                                                                                                                                  >  SRR19973065.3200198/1‑150 (MQ=60)
  CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCT                                                                                                                                                  >  SRR19973065.432504/1‑150 (MQ=60)
  CAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCT                                                                                                                                                  >  SRR19973065.432516/1‑150 (MQ=60)
      CGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCG                                                                                                                                              <  SRR19973065.2691049/150‑1 (MQ=60)
      AGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCG                                                                                                                                              <  SRR19973065.1800021/150‑1 (MQ=60)
        ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCT                                                                                                                                            >  SRR19973065.1398543/1‑150 (MQ=60)
        ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCT                                                                                                                                            >  SRR19973065.896606/1‑150 (MQ=60)
        ATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCT                                                                                                                                            <  SRR19973065.2614799/150‑1 (MQ=60)
          CTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTG                                                                                                                                          <  SRR19973065.1592715/150‑1 (MQ=60)
           TGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGC                                                                                                                                         <  SRR19973065.1184549/150‑1 (MQ=60)
                ATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAA                                                                                                                                    >  SRR19973065.933748/1‑150 (MQ=60)
                 TCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAA                                                                                                                                   >  SRR19973065.2212367/1‑150 (MQ=60)
                 TCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAA                                                                                                                                   >  SRR19973065.2212363/1‑150 (MQ=60)
                   ACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACG                                                                                                                                 <  SRR19973065.2716662/150‑1 (MQ=60)
                      CAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGC                                                                                                                              >  SRR19973065.1225774/1‑150 (MQ=60)
                         CCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTC                                                                                                                           <  SRR19973065.324085/150‑1 (MQ=60)
                         CCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTC                                                                                                                           <  SRR19973065.2832473/150‑1 (MQ=60)
                         CCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTC                                                                                                                           <  SRR19973065.2832799/150‑1 (MQ=60)
                          CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                          >  SRR19973065.1798975/1‑150 (MQ=60)
                          CACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCA                                                                                                                          <  SRR19973065.3424170/150‑1 (MQ=60)
                            CAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGG                                                                                                                        <  SRR19973065.259644/150‑1 (MQ=60)
                             AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                       <  SRR19973065.1474057/150‑1 (MQ=60)
                             AACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGT                                                                                                                       <  SRR19973065.1026905/150‑1 (MQ=60)
                                    TGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGG                                                                                                                >  SRR19973065.2808298/1‑150 (MQ=60)
                                        GAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTA                                                                                                            <  SRR19973065.3488692/150‑1 (MQ=60)
                                           CGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATC                                                                                                         <  SRR19973065.1060674/150‑1 (MQ=60)
                                            GCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCA                                                                                                        >  SRR19973065.2666589/1‑150 (MQ=60)
                                              CAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAAT                                                                                                      >  SRR19973065.2651684/1‑150 (MQ=60)
                                               AGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATA                                                                                                     <  SRR19973065.2837458/150‑1 (MQ=60)
                                                      CACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACACGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCA                                                                                              <  SRR19973065.3304976/150‑1 (MQ=60)
                                                      CACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCA                                                                                              <  SRR19973065.2445028/150‑1 (MQ=60)
                                                       ACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCACAC‑CGTACCTCTATCTATTTCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCAC                                                                                             <  SRR19973065.3468708/150‑1 (MQ=60)
                                                        CCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACC                                                                                            <  SRR19973065.2064052/150‑1 (MQ=60)
                                                            CGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCTCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAC                                                                                        >  SRR19973065.1247398/1‑150 (MQ=60)
                                                            CGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTAC                                                                                        >  SRR19973065.1247442/1‑150 (MQ=60)
                                                              GAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAT                                                                                      >  SRR19973065.981983/1‑150 (MQ=60)
                                                              GAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAT                                                                                      >  SRR19973065.2746554/1‑150 (MQ=60)
                                                              GAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAT                                                                                      >  SRR19973065.659827/1‑150 (MQ=60)
                                                               AATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATT                                                                                     <  SRR19973065.2986506/150‑1 (MQ=60)
                                                                 TCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGG                                                                                   >  SRR19973065.1077001/1‑150 (MQ=60)
                                                                 TCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGG                                                                                   <  SRR19973065.451508/150‑1 (MQ=60)
                                                                    CCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTG                                                                                <  SRR19973065.3086466/150‑1 (MQ=60)
                                                                       GCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAG                                                                             <  SRR19973065.1295153/150‑1 (MQ=60)
                                                                        CAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGC                                                                            >  SRR19973065.2193899/1‑150 (MQ=60)
                                                                          AAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTT                                                                          <  SRR19973065.1012770/150‑1 (MQ=60)
                                                                               AATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAG                                                                     >  SRR19973065.1197534/1‑150 (MQ=60)
                                                                                ATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGC                                                                    >  SRR19973065.1549056/1‑150 (MQ=60)
                                                                                   CGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGG                                                                 <  SRR19973065.3237563/150‑1 (MQ=60)
                                                                                   CGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGG                                                                 >  SRR19973065.1241457/1‑150 (MQ=60)
                                                                                   CGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGG                                                                 <  SRR19973065.3240784/150‑1 (MQ=60)
                                                                                       ACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCA                                                             >  SRR19973065.447048/1‑150 (MQ=60)
                                                                                       ACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGG‑AAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            >  SRR19973065.748912/1‑150 (MQ=60)
                                                                                        CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            <  SRR19973065.341698/150‑1 (MQ=60)
                                                                                        CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            >  SRR19973065.1471196/1‑150 (MQ=60)
                                                                                        CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            >  SRR19973065.1032332/1‑150 (MQ=60)
                                                                                        CATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCAT                                                            >  SRR19973065.1469851/1‑150 (MQ=60)
                                                                                         ATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACCAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATG                                                           <  SRR19973065.1599141/150‑1 (MQ=60)
                                                                                         ATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATG                                                           <  SRR19973065.1599146/150‑1 (MQ=60)
                                                                                               ATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGA                                                     >  SRR19973065.1755266/1‑150 (MQ=60)
                                                                                               ATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGA                                                     >  SRR19973065.488578/1‑150 (MQ=60)
                                                                                               ATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGA                                                     >  SRR19973065.939717/1‑150 (MQ=60)
                                                                                                 TTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATC                                                   <  SRR19973065.2626934/150‑1 (MQ=60)
                                                                                                 TTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATC                                                   <  SRR19973065.2627554/150‑1 (MQ=60)
                                                                                                  TGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCG                                                  >  SRR19973065.3066131/1‑150 (MQ=60)
                                                                                                    ATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTG                                                >  SRR19973065.2000904/1‑150 (MQ=60)
                                                                                                      AATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGG                                              <  SRR19973065.2480581/150‑1 (MQ=60)
                                                                                                       ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             <  SRR19973065.68704/150‑1 (MQ=60)
                                                                                                       ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             >  SRR19973065.2831221/1‑150 (MQ=60)
                                                                                                       ATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGT                                             <  SRR19973065.348279/150‑1 (MQ=60)
                                                                                                        TCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTA                                            >  SRR19973065.397929/1‑150 (MQ=60)
                                                                                                        TCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTA                                            >  SRR19973065.397928/1‑150 (MQ=60)
                                                                                                        TCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTA                                            <  SRR19973065.1495392/150‑1 (MQ=60)
                                                                                                          CTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATC                                          >  SRR19973065.1369768/1‑150 (MQ=60)
                                                                                                           TCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCG                                         >  SRR19973065.535374/1‑150 (MQ=60)
                                                                                                           TCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTCGCTGTAGCTTGCCAGCAGGCGCATGAAGCGATCGTGGGTATCG                                         >  SRR19973065.1782008/1‑150 (MQ=60)
                                                                                                             CGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAG                                       <  SRR19973065.1320299/150‑1 (MQ=60)
                                                                                                             CGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAG                                       <  SRR19973065.1320298/150‑1 (MQ=60)
                                                                                                               CCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAT                                     >  SRR19973065.2742997/1‑150 (MQ=60)
                                                                                                                CTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATT                                    <  SRR19973065.1354876/150‑1 (MQ=60)
                                                                                                                 TGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTG                                   >  SRR19973065.2010301/1‑150 (MQ=60)
                                                                                                                 TGCTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTG                                   <  SRR19973065.470780/150‑1 (MQ=60)
                                                                                                                   CTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCC                                 <  SRR19973065.2514884/150‑1 (MQ=60)
                                                                                                                   CTGGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCC                                 >  SRR19973065.3083222/1‑150 (MQ=60)
                                                                                                                     GGTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGA                               <  SRR19973065.3428547/150‑1 (MQ=60)
                                                                                                                      GTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAG                              <  SRR19973065.69868/150‑1 (MQ=60)
                                                                                                                      GTGGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAG                              <  SRR19973065.619958/150‑1 (MQ=60)
                                                                                                                       TGGAAATAACAACAAGCAGC‑CACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGA                             <  SRR19973065.1294959/150‑1 (MQ=60)
                                                                                                                        GGAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAA                            >  SRR19973065.2455451/1‑150 (MQ=60)
                                                                                                                         GAAATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAAT                           <  SRR19973065.2979623/150‑1 (MQ=60)
                                                                                                                           AATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAA                         >  SRR19973065.612144/1‑150 (MQ=60)
                                                                                                                            ATAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAA                        <  SRR19973065.1983633/150‑1 (MQ=60)
                                                                                                                             TAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAA                       >  SRR19973065.1956128/1‑150 (MQ=60)
                                                                                                                             TAACAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAA                       >  SRR19973065.625460/1‑150 (MQ=60)
                                                                                                                               ACAACAAGCAGA‑CACATTTACCTCTATCGCTTGCGTAAAACGCGCTTCAGGTGAAATGTTGTAATCAATTCAAATCACCGTACTTTGGCTGTAGCTTGCCAGCAGGTGCATGATGCGATCGTGGGTATCTAGATTGCCGAGATTAAAACC                     <  SRR19973065.3116395/150‑1 (MQ=60)
                                                                                                                                CAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                    <  SRR19973065.395694/150‑1 (MQ=60)
                                                                                                                                CAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                    <  SRR19973065.395693/150‑1 (MQ=60)
                                                                                                                                CAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                    <  SRR19973065.395529/150‑1 (MQ=60)
                                                                                                                                CAACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCG                    >  SRR19973065.2677680/1‑150 (MQ=60)
                                                                                                                                 AACAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGC                   >  SRR19973065.796327/1‑150 (MQ=60)
                                                                                                                                   CAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 >  SRR19973065.1923484/1‑150 (MQ=60)
                                                                                                                                   CAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 >  SRR19973065.784757/1‑150 (MQ=60)
                                                                                                                                   CAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 >  SRR19973065.784882/1‑150 (MQ=60)
                                                                                                                                   CAAGCAGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCT                 <  SRR19973065.3336648/150‑1 (MQ=60)
                                                                                                                                        AGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGTCGAGAATAAAACCGCCTCCATG            >  SRR19973065.3042318/1‑150 (MQ=60)
                                                                                                                                        AGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGTCGAGAATAAAACCGCCTCCATG            >  SRR19973065.813206/1‑150 (MQ=60)
                                                                                                                                        AGC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGTCGAGAATAAAACCGCCTCCATG            >  SRR19973065.813214/1‑150 (MQ=60)
                                                                                                                                         GC‑CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGC           >  SRR19973065.3165997/1‑150 (MQ=60)
                                                                                                                                            CACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCTGGCGCTTGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAA         <  SRR19973065.444717/150‑1 (MQ=60)
                                                                                                                                              CAATTCCCTCTCTGGCTTGCGGAAAACGCGCTTCTGTTGAAAGGTTGTAATCAATACCATTCACGGTACTTGGGCTGTAGGTTGCCAGCCGGCGCATTATGCGTTCGTGGGTATCTAGTTTGCCGAGAATAAAACCGCCTCCATGCAAAT       <  SRR19973065.2151933/150‑1 (MQ=60)
                                                                                                                                              CAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT       <  SRR19973065.3064656/150‑1 (MQ=60)
                                                                                                                                              CAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAAT       <  SRR19973065.2922941/150‑1 (MQ=60)
                                                                                                                                               AATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATA      >  SRR19973065.1613384/1‑150 (MQ=60)
                                                                                                                                                ATTACCTCTATCTGTTGCTGAAAACGCGCTTCTGGTGAATGGGTGTAATCAATACAAATCACCGTAAATTGGCTTTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGATAATAAAACCGCCTCCATGCAAATAA     <  SRR19973065.1944889/150‑1 (MQ=60)
                                                                                                                                                ATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAA     <  SRR19973065.2332206/150‑1 (MQ=60)
                                                                                                                                                   ACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATGGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  <  SRR19973065.2497618/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGC‑CACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAAAT  >  CP009273/494874‑495169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑