Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 100% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT97.4% 597.2 / 4.6 189L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (95/89);  total (99/90)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACC  >  CP009273/1821551‑1821839
                                                                                                                                                 |                                                                                                                                                
aGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGt                                                                                                                                                >  1:1154670/1‑148 (MQ=255)
aGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGt                                                                                                                                                >  1:1156436/1‑148 (MQ=255)
    agcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACt                                                                                                                                            <  2:3186472/148‑1 (MQ=255)
      caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                          >  1:2075010/1‑148 (MQ=255)
           aCATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTg                                                                                                                                     >  2:101634/1‑148 (MQ=255)
                atGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgag                                                                                                                                >  2:2448416/1‑148 (MQ=255)
                 tGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagc                                                                                                                               >  2:3554352/1‑148 (MQ=255)
                    aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGaa                                                                                                                            <  2:3431129/148‑1 (MQ=255)
                     aCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAc                                                                                                                           >  2:898639/1‑148 (MQ=255)
                      ccATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACg                                                                                                                          >  2:415302/1‑148 (MQ=255)
                        atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGcc                                                                                                                        <  1:962963/148‑1 (MQ=255)
                        atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGcc                                                                                                                        >  2:2471016/1‑148 (MQ=255)
                        atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGcc                                                                                                                        <  1:1620751/148‑1 (MQ=255)
                         tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGACCGCCt                                                                                                                       >  1:3445182/1‑148 (MQ=255)
                         tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCt                                                                                                                       >  2:1858596/1‑148 (MQ=255)
                          catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                      >  2:3344079/1‑148 (MQ=255)
                          catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                      >  2:2347992/1‑148 (MQ=255)
                          catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                      <  2:1751378/148‑1 (MQ=255)
                           atcGCGGCTCAGCATTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACTGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                     <  1:2183230/148‑1 (MQ=255)
                            tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                    >  2:2690972/1‑148 (MQ=255)
                            tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                    >  1:1960377/1‑148 (MQ=255)
                             cgcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtg                                                                                                                   >  1:3160043/1‑148 (MQ=255)
                             cgcgGCTCAGCAGTTGGCTGTTAAACAGAGAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtg                                                                                                                   <  2:459039/148‑1 (MQ=255)
                              gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtgg                                                                                                                  >  2:1916057/1‑148 (MQ=255)
                               cgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggt                                                                                                                 >  1:1050197/1‑148 (MQ=255)
                                  cTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGc                                                                                                              >  2:2783096/1‑148 (MQ=255)
                                    cagcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaa                                                                                                            >  2:2415218/1‑148 (MQ=255)
                                          ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGc                                                                                                     <  1:2281541/148‑1 (MQ=255)
                                            ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCAc                                                                                                   <  2:359132/148‑1 (MQ=255)
                                              cTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCt                                                                                                 >  2:1204675/1‑148 (MQ=255)
                                                 ttAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGc                                                                                              >  1:1978338/1‑148 (MQ=255)
                                                  tAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCg                                                                                             >  1:503414/1‑148 (MQ=255)
                                                    aaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGt                                                                                           <  1:2285264/148‑1 (MQ=255)
                                                    aaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGt                                                                                           >  2:287706/1‑148 (MQ=255)
                                                      cAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTa                                                                                         >  2:1345643/1‑148 (MQ=255)
                                                        gATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACt                                                                                       >  2:3416479/1‑148 (MQ=255)
                                                           aGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCAc                                                                                    <  2:1155351/148‑1 (MQ=255)
                                                            ggTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACg                                                                                   >  2:1873073/1‑148 (MQ=255)
                                                               aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCa                                                                                <  2:2153570/148‑1 (MQ=255)
                                                                gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCaa                                                                               >  2:312355/1‑148 (MQ=255)
                                                                 acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAAt                                                                              <  1:1712783/148‑1 (MQ=255)
                                                                  cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATc                                                                             >  1:17454/1‑148 (MQ=255)
                                                                  cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATc                                                                             >  1:3321333/1‑148 (MQ=255)
                                                                   acCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCa                                                                            >  1:2983483/1‑148 (MQ=255)
                                                                     cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACg                                                                          <  2:1051837/148‑1 (MQ=255)
                                                                     cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACg                                                                          <  2:1747546/148‑1 (MQ=255)
                                                                        aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                       <  2:1435547/148‑1 (MQ=255)
                                                                        aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                       <  1:926570/148‑1 (MQ=255)
                                                                        aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                       <  1:2793562/148‑1 (MQ=255)
                                                                          cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGGCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                     >  1:2816370/1‑148 (MQ=255)
                                                                          cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                     >  2:2507273/1‑148 (MQ=255)
                                                                           aGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTa                                                                    <  1:2643093/148‑1 (MQ=255)
                                                                            gACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAt                                                                   >  2:553122/1‑148 (MQ=255)
                                                                             acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                  >  1:3548485/1‑148 (MQ=255)
                                                                               acGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaa                                                                >  1:2906775/1‑148 (MQ=255)
                                                                               acGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTCTGACTGGCGAGCGAAAGCCTGTTGTTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaa                                                                >  2:299384/1‑148 (MQ=255)
                                                                                cGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaa                                                               <  1:1496237/148‑1 (MQ=255)
                                                                                cGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaa                                                               >  1:3309467/1‑148 (MQ=255)
                                                                                  gAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaaa                                                             <  1:1135488/148‑1 (MQ=255)
                                                                                    aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACg                                                           <  1:828147/148‑1 (MQ=255)
                                                                                       cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGccc                                                        <  2:2091133/148‑1 (MQ=255)
                                                                                       cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGccc                                                        >  1:58360/1‑148 (MQ=255)
                                                                                         gAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCCTTATGAAAAACGCCCTg                                                      >  2:2024354/1‑148 (MQ=255)
                                                                                         gAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTg                                                      >  1:3328685/1‑148 (MQ=255)
                                                                                              gCCGTAAGTTCTATCGCCATAAAACAATTGACCCGCGCACGCTGTTTTGCCTGTAACTGTGACTGGCGAGCTAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGAAATCACGTAATTATGAAAAACGCCCTGGTCGa                                                 <  1:2118706/148‑1 (MQ=255)
                                                                                              gCCGGAACTTCTATCGCCAAAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCGGGGGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGa                                                 <  1:63077/148‑1 (MQ=255)
                                                                                              gCCGGAACTTCTATCCCCATAAAACCATGGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAGTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGa                                                 <  1:2118669/148‑1 (MQ=255)
                                                                                               ccGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAt                                                >  1:30680/1‑148 (MQ=255)
                                                                                                cGGAACTTCTATCGCCATAAAACCATTGACCCGCGCCCGCAGTTTTGCCTTTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATa                                               <  1:3362666/148‑1 (MQ=255)
                                                                                                    aCTTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGt                                           <  1:2836830/148‑1 (MQ=255)
                                                                                                      ttCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTcc                                         >  1:2217027/1‑148 (MQ=255)
                                                                                                       tCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                        <  2:65936/148‑1 (MQ=255)
                                                                                                         tATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCggg                                      <  2:3225175/148‑1 (MQ=255)
                                                                                                          aTCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGt                                     >  2:1040070/1‑148 (MQ=255)
                                                                                                               cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCCCGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGc                                <  2:2075010/148‑1 (MQ=255)
                                                                                                                aTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                               >  1:3362282/1‑148 (MQ=255)
                                                                                                                 tAAAACCTTTGACCCGCGCACGATGTTTTGCCTGTAACTTTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCCCCTGGCGGTTACTCACGGAAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                              <  2:629243/148‑1 (MQ=255)
                                                                                                                     aCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCg                          >  1:2318874/1‑148 (MQ=255)
                                                                                                                        aTTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaa                       <  1:2348037/148‑1 (MQ=255)
                                                                                                                         ttGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                      >  1:1001456/1‑148 (MQ=255)
                                                                                                                         ttGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                      >  1:1001439/1‑148 (MQ=255)
                                                                                                                         ttGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                      >  1:2865617/1‑148 (MQ=255)
                                                                                                                         tgcccccGCGCACGCAGTTTTGCCTGTACCTGTGAGGGGCGAGCGACCGCCTGTTGGTGGAAAAA‑CCGCACGGGGCGTTTACCCCCGGAAATCACTTCATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                      <  1:1548959/144‑1 (MQ=255)
                                                                                                                                gcgcACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTTTGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTg               <  1:2934272/148‑1 (MQ=255)
                                                                                                                                gcgcACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTg               <  2:2949596/148‑1 (MQ=255)
                                                                                                                                gcgcACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTg               <  2:381889/148‑1 (MQ=255)
                                                                                                                                 cgcacgctGGTTTGCCTGTACCTGTGACTGGCGAGCGACCGCCTGTGGGTGGAAAAA‑CCCCACCTGCCGGTTACCCACGGCAATCACGTCATAATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt              <  1:2506539/148‑1 (MQ=255)
                                                                                                                                 cgcacgctGGTTTGCCTGTAACGGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCGGGCGGTTACTCCCGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt              <  2:340120/148‑1 (MQ=255)
                                                                                                                                 cgcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt              <  2:3309467/148‑1 (MQ=255)
                                                                                                                                 cgcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt              <  2:692842/148‑1 (MQ=255)
                                                                                                                                 cgcacgcaGGTTTGCCTGAAACTGTGACGGGCGAGCGAACGCCTTTTGG‑GGCAAAACCAGCACCGGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt              <  1:3359588/148‑1 (MQ=255)
                                                                                                                                  gcacgctGGTTTGCCTTTAACTGTGACGGGCGAGCGACCGCCTGTTGGGGGAAAAA‑CAGCACCTGGGGTTTACTCACGGCAATCACTAAATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt             <  1:755361/148‑1 (MQ=255)
                                                                                                                                  gcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt             >  2:1660119/1‑148 (MQ=255)
                                                                                                                                  gcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt             <  1:3235654/148‑1 (MQ=255)
                                                                                                                                     cgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTggg          >  2:3172462/1‑148 (MQ=255)
                                                                                                                                      gcaGGTTTGCCTTTAAGTGTGACTGGCGAGCGAACGCCTGTGGGTGGAAAAA‑CAGCACCGGGGGGTTACTCACGGAAATCACGTCTTTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgggg         <  1:2448416/148‑1 (MQ=255)
                                                                                                                                       caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGa        >  2:3435800/1‑148 (MQ=255)
                                                                                                                                         ggTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt      >  1:1810962/1‑148 (MQ=255)
                                                                                                                                             tGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAcc  >  2:1303392/1‑148 (MQ=255)
                                                                                                                                             tGCCTGTAACTGTGACTGGCGAGCGAACGCCTGATGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAcc  >  2:1271192/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACC  >  CP009273/1821551‑1821839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATTC  >  CP009273/1821547‑1821845
                                                                                                                                                     |                                                                                                                                                      
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCT                                                                                                                                                        <  SRR19973067.1758725/150‑1 (MQ=60)
 CTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTG                                                                                                                                                       >  SRR19973067.342455/1‑150 (MQ=60)
 CTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTG                                                                                                                                                       >  SRR19973067.1653421/1‑150 (MQ=60)
  TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGT                                                                                                                                                      >  SRR19973067.1787758/1‑150 (MQ=60)
  TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGT                                                                                                                                                      >  SRR19973067.1162368/1‑150 (MQ=60)
  TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGT                                                                                                                                                      >  SRR19973067.1162381/1‑150 (MQ=60)
  TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGT                                                                                                                                                      >  SRR19973067.1164151/1‑150 (MQ=60)
    AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAA                                                                                                                                                    >  SRR19973067.1081991/1‑150 (MQ=60)
      GCAGCACCAACATCATGGAACCATCATCGCGGCTCCGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACT                                                                                                                                                  <  SRR19973067.1985417/150‑1 (MQ=60)
       CAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTG                                                                                                                                                 >  SRR19973067.201819/1‑150 (MQ=60)
        AGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGT                                                                                                                                                >  SRR19973067.2088785/1‑150 (MQ=60)
          CACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGA                                                                                                                                              >  SRR19973067.1163063/1‑150 (MQ=60)
           ACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAC                                                                                                                                             <  SRR19973067.311403/150‑1 (MQ=60)
                           CATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGACCGCCT                                                                                                                             >  SRR19973067.3467106/1‑150 (MQ=60)
                            ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                            <  SRR19973067.1631872/150‑1 (MQ=60)
                            ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                            <  SRR19973067.969560/150‑1 (MQ=60)
                            ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                            <  SRR19973067.968824/150‑1 (MQ=60)
                              CATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTT                                                                                                                          >  SRR19973067.1973702/1‑150 (MQ=60)
                               ATCGCGGCTCAGCATTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACTGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTG                                                                                                                         <  SRR19973067.2197827/150‑1 (MQ=60)
                               ATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTG                                                                                                                         >  SRR19973067.3180066/1‑150 (MQ=60)
                                 CGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGT                                                                                                                       <  SRR19973067.798152/150‑1 (MQ=60)
                                 CGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGT                                                                                                                       <  SRR19973067.2325956/150‑1 (MQ=60)
                                 CGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGT                                                                                                                       >  SRR19973067.1057402/1‑150 (MQ=60)
                                         AGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑C                                                                                                              >  SRR19973067.1557475/1‑150 (MQ=60)
                                              TTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCAC                                                                                                         <  SRR19973067.2296627/150‑1 (MQ=60)
                                               TGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACC                                                                                                        >  SRR19973067.3307430/1‑150 (MQ=60)
                                                   TGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGC                                                                                                    >  SRR19973067.1991738/1‑150 (MQ=60)
                                                    GTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCG                                                                                                   >  SRR19973067.506862/1‑150 (MQ=60)
                                                      TAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGT                                                                                                 >  SRR19973067.3247754/1‑150 (MQ=60)
                                                      TAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGT                                                                                                 >  SRR19973067.1678842/1‑150 (MQ=60)
                                                        AACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTA                                                                                               <  SRR19973067.2300368/150‑1 (MQ=60)
                                                        AACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTA                                                                                               >  SRR19973067.2104963/1‑150 (MQ=60)
                                                               AGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTCGCTAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCTGTTACTaaccg                                                                                        >  SRR19973067.2299908/1‑145 (MQ=60)
                                                                    GACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATC                                                                                   >  SRR19973067.3342342/1‑150 (MQ=60)
                                                                    GACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATC                                                                                   >  SRR19973067.17719/1‑150 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCA                                                                                  >  SRR19973067.3002336/1‑150 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCA                                                                                  <  SRR19973067.3493508/150‑1 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCA                                                                                  <  SRR19973067.1724531/150‑1 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCA                                                                                  >  SRR19973067.3002351/1‑150 (MQ=60)
                                                                           TATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCAT                                                                            >  SRR19973067.2198186/1‑150 (MQ=60)
                                                                            ATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGGCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATT                                                                           >  SRR19973067.2833910/1‑150 (MQ=60)
                                                                            ATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGGCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATT                                                                           >  SRR19973067.2834508/1‑150 (MQ=60)
                                                                            ATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATT                                                                           <  SRR19973067.2811589/150‑1 (MQ=60)
                                                                            ATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATT                                                                           <  SRR19973067.932815/150‑1 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                        >  SRR19973067.3570867/1‑150 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                        <  SRR19973067.2579203/150‑1 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                        <  SRR19973067.2660165/150‑1 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                        <  SRR19973067.2660166/150‑1 (MQ=60)
                                                                                 ACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAA                                                                      >  SRR19973067.2925298/1‑150 (MQ=60)
                                                                                  CACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAA                                                                     >  SRR19973067.3330360/1‑150 (MQ=60)
                                                                                    CGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAA                                                                   <  SRR19973067.2858623/150‑1 (MQ=60)
                                                                                    CGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAA                                                                   <  SRR19973067.1506106/150‑1 (MQ=60)
                                                                                     GGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAC                                                                  <  SRR19973067.3464355/150‑1 (MQ=60)
                                                                                      GAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACG                                                                 <  SRR19973067.1143138/150‑1 (MQ=60)
                                                                                      GAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACG                                                                 <  SRR19973067.159017/150‑1 (MQ=60)
                                                                                       AAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGC                                                                >  SRR19973067.1289213/1‑150 (MQ=60)
                                                                                        AACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCC                                                               <  SRR19973067.833796/150‑1 (MQ=60)
                                                                                         ACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCC                                                              >  SRR19973067.59082/1‑150 (MQ=60)
                                                                                           CTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTG                                                            >  SRR19973067.3349090/1‑150 (MQ=60)
                                                                                           CTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTG                                                            >  SRR19973067.3349740/1‑150 (MQ=60)
                                                                                                 TGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAT                                                      >  SRR19973067.31144/1‑150 (MQ=60)
                                                                                                  GCCGTAAGTTCTATCGCCATAAAACAATTGACCCGCGCACGCTGTTTTGCCTGTAACTGTGACTGGCGAGCTAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGAAATCACGTAATTATGAAAAACGCCCTGGTCGATA                                                     <  SRR19973067.2132834/150‑1 (MQ=60)
                                                                                                  GCCGTAAATTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGAAATAACGTCATTATGAAAAACGCCCTGGTCGATA                                                     <  SRR19973067.2132852/150‑1 (MQ=60)
                                                                                                  GCCGGAACTTCTATCGCCAAAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCGGGGGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATA                                                     <  SRR19973067.63835/150‑1 (MQ=60)
                                                                                                  GCCGGAACTTCTATCCCCATAAAACCATGGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAGTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATA                                                     <  SRR19973067.2132796/150‑1 (MQ=60)
                                                                                                  GCCGGAAATTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATA                                                     <  SRR19973067.2132798/150‑1 (MQ=60)
                                                                                                    CGTAAATTCTATCGACATAAAACCATTGACCCGCGCACGCTGGTTTGCATGTACCTGTGACTGGCGTGCGACCGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGTTTACTCACGGAAATCACTTAATTATGAAAAACGCCCTGGTCGATAAC                                                   <  SRR19973067.1005600/150‑1 (MQ=60)
                                                                                                    CGGAACTTCTATCGCCATAAAACCATTGACCCGCGCCCGCAGTTTTGCCTTTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAAC                                                   <  SRR19973067.3384011/150‑1 (MQ=60)
                                                                                                    CGGAACTTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAC                                                   <  SRR19973067.3383934/150‑1 (MQ=60)
                                                                                                        ACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCC                                               >  SRR19973067.2231794/1‑150 (MQ=60)
                                                                                                        ACTTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCC                                               <  SRR19973067.2855057/150‑1 (MQ=60)
                                                                                                               TCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTT                                        <  SRR19973067.245760/150‑1 (MQ=60)
                                                                                                               GCGCCATAAAACCTTTGCCCCGCGCACGCTGGTTTGCCTGTAAGTGTGAGGGGCGAGCGAACGCCTGTTGGGGGAAAAA‑CCGCACCGGGGGGTTACACCCGGAAATCCCTTCATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTT                                        <  SRR19973067.3364552/150‑1 (MQ=51)
                                                                                                                  CCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCT                                     >  SRR19973067.3383626/1‑150 (MQ=60)
                                                                                                                     TAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGG                                  >  SRR19973067.189359/1‑150 (MQ=60)
                                                                                                                       AAACCATTGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCG                                <  SRR19973067.297591/150‑1 (MQ=60)
                                                                                                                       AAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCG                                >  SRR19973067.2334126/1‑150 (MQ=60)
                                                                                                                       AAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCG                                >  SRR19973067.889148/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                            >  SRR19973067.1008322/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                            >  SRR19973067.1008339/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                            >  SRR19973067.2884246/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                            >  SRR19973067.2883972/1‑150 (MQ=60)
                                                                                                                            ATTGCCCCGCGCCCGCTGGTTGGCCTGTACCTGTGACGGGGGAGCGACCGCCTGTTGGTGGAAAAA‑CCGCACCTGGCGGTTACTCACGGAAATCACGTAATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAT                           <  SRR19973067.102658/150‑1 (MQ=60)
                                                                                                                            ATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAT                           <  SRR19973067.2363328/150‑1 (MQ=60)
                                                                                                                            ATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAT                           <  SRR19973067.2363373/150‑1 (MQ=60)
                                                                                                                             tgccCCCGCGCACGCAGTTTTGCCTGTACCTGTGAGGGGCGAGCGACCGCCTGTTGGTGGAAAAA‑CCGCACGGGGCGTTTACCCCCGGAAATCACTTCATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATC                          <  SRR19973067.1559497/146‑1 (MQ=60)
                                                                                                                                CCCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAA‑CCGCACCTGGGGGTTACTCACGGCAATCACGTCATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACC                       <  SRR19973067.2476542/150‑1 (MQ=60)
                                                                                                                                 CCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                      <  SRR19973067.3576757/150‑1 (MQ=60)
                                                                                                                                    GCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTTTGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTT                   <  SRR19973067.2952893/150‑1 (MQ=60)
                                                                                                                                     CGCACGCTGGTTTGCCTGTACCTGTGACTGGCGAGCGACCGCCTGTGGGTGGAAAAA‑CCCCACCTGCCGGTTACCCACGGCAATCACGTCATAATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                  <  SRR19973067.2522983/150‑1 (MQ=60)
                                                                                                                                     CGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGCCAAAA‑CCGCACCTGGCGTTTACTCACGGAAATCACGTCTTTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                  <  SRR19973067.3381608/150‑1 (MQ=60)
                                                                                                                                     CGCACGCAGGTTTGCCTGAAACTGTGACGGGCGAGCGAACGCCTTTTGG‑GGCAAAACCAGCACCGGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                  <  SRR19973067.3380918/150‑1 (MQ=60)
                                                                                                                                      GCACGCTGGTTTGCCTTTAAGTGTGAGTGGCGAGCGAACGCGTGTTGGTGGAAAAA‑CAGCACGTGGGGGTTACTCACGTAAATCACTTCTTTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGG                 <  SRR19973067.760087/150‑1 (MQ=60)
                                                                                                                                      GCACGCTGGTTTGCCTTTAACTGTGACGGGCGAGCGACCGCCTGTTGGGGGAAAAA‑CAGCACCTGGGGTTTACTCACGGCAATCACTAAATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGG                 <  SRR19973067.760613/150‑1 (MQ=60)
                                                                                                                                      GCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGG                 <  SRR19973067.3255949/150‑1 (MQ=60)
                                                                                                                                          GCAGGTTTGCCTTTAAGTGTGACTGGCGAGCGAACGCCTGTGGGTGGAAAAA‑CAGCACCGGGGGGTTACTCACGGAAATCACGTCTTTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAT             <  SRR19973067.2464502/150‑1 (MQ=60)
                                                                                                                                           CAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATT            >  SRR19973067.1823209/1‑150 (MQ=60)
                                                                                                                                             GTTTTGCCTGTAACTGTGACTGGGGTGCGAACGCCTTTTGGTGGAAAAA‑AAGCACCTGGCGGTTACTCACGGCAATCACTTCATTATGAAAAACGCCCTGGTCTATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCA          <  SRR19973067.294570/150‑1 (MQ=60)
                                                                                                                                               TTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACC        >  SRR19973067.569404/1‑150 (MQ=60)
                                                                                                                                                     TGTAACTGTGACTGGCGAGCGACCGCCTGTTGGGGGCAAAA‑CCGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATTC  <  SRR19973067.97969/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA‑CAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATTC  >  CP009273/1821547‑1821845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑