Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 100% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT99.5% 616.6 / ‑5.2 190L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (95/94);  total (95/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG  >  CP009273/1821552‑1821841
                                                                                                                                                |                                                                                                                                                 
ggCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTa                                                                                                                                                <  1:2466901/148‑1 (MQ=255)
     caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                           <  1:1131401/148‑1 (MQ=255)
     caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                           >  2:1350698/1‑148 (MQ=255)
        cAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAc                                                                                                                                        <  1:2616266/148‑1 (MQ=255)
        cAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAc                                                                                                                                        >  2:1233293/1‑148 (MQ=255)
           catcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTgg                                                                                                                                     >  2:2983103/1‑148 (MQ=255)
           catcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTgg                                                                                                                                     >  2:1858200/1‑148 (MQ=255)
                 ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagcg                                                                                                                               >  2:1851963/1‑148 (MQ=255)
                         catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                       >  1:3454244/1‑148 (MQ=255)
                         catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                       >  1:3455171/1‑148 (MQ=255)
                          atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                      >  1:1449161/1‑148 (MQ=255)
                          atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                      >  2:853464/1‑148 (MQ=255)
                          atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                      >  2:2612074/1‑148 (MQ=255)
                           tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                     >  2:3066415/1‑148 (MQ=255)
                           tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                     >  1:1094227/1‑148 (MQ=255)
                           tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                     <  2:794308/148‑1 (MQ=255)
                             gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtgg                                                                                                                   <  1:414130/148‑1 (MQ=255)
                              cgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggt                                                                                                                  <  2:1242473/148‑1 (MQ=255)
                                gCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggtgg                                                                                                                >  1:1662282/1‑148 (MQ=255)
                                    agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaaa                                                                                                            <  1:1327909/148‑1 (MQ=255)
                                     gcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaaaa                                                                                                           <  2:2769870/148‑1 (MQ=255)
                                      cagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAc                                                                                                          >  1:1055059/1‑148 (MQ=255)
                                      cagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAc                                                                                                          <  1:1289543/148‑1 (MQ=255)
                                         ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGc                                                                                                       <  2:1019819/148‑1 (MQ=255)
                                            gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                    <  1:1173618/148‑1 (MQ=255)
                                            gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                    >  1:2028689/1‑148 (MQ=255)
                                            gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                    <  2:1439927/148‑1 (MQ=255)
                                             cTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                   <  1:1021802/148‑1 (MQ=255)
                                              tGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTg                                                                                                  <  2:2162319/148‑1 (MQ=255)
                                               gTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTgg                                                                                                 >  1:1791571/1‑148 (MQ=255)
                                                ttAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAATACAGCACCTGGc                                                                                                >  1:595783/1‑148 (MQ=255)
                                                ttAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGc                                                                                                >  1:624524/1‑148 (MQ=255)
                                                   aaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGt                                                                                             >  1:722161/1‑148 (MQ=255)
                                                        aTAGGTAGACACCGTATCAGACACTGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTc                                                                                        >  1:2682907/1‑148 (MQ=255)
                                                        aTAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTc                                                                                        >  1:2303631/1‑148 (MQ=255)
                                                         tAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCa                                                                                       >  2:2890467/1‑148 (MQ=255)
                                                             tAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGc                                                                                   >  2:1951124/1‑148 (MQ=255)
                                                              aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCa                                                                                  <  1:1572763/148‑1 (MQ=255)
                                                              aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCa                                                                                  <  1:1396842/148‑1 (MQ=255)
                                                              aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCa                                                                                  <  2:2998983/148‑1 (MQ=255)
                                                               gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCaa                                                                                 >  1:1751134/1‑148 (MQ=255)
                                                               gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCaa                                                                                 >  2:2252169/1‑148 (MQ=255)
                                                                acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                                >  1:2218515/1‑148 (MQ=255)
                                                                acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                                <  2:414961/148‑1 (MQ=255)
                                                                 cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                               <  2:830108/148‑1 (MQ=255)
                                                                  acCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATGGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCa                                                                              <  2:40670/148‑1 (MQ=255)
                                                                   ccGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGACCGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCAc                                                                             >  2:1652514/1‑148 (MQ=255)
                                                                        tCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAt                                                                        >  1:2907296/1‑148 (MQ=255)
                                                                         cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                       <  1:392811/148‑1 (MQ=255)
                                                                           gACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAt                                                                     >  1:2866758/1‑148 (MQ=255)
                                                                            acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                    <  2:497993/148‑1 (MQ=255)
                                                                            acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                    >  2:2564369/1‑148 (MQ=255)
                                                                            acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                    >  2:2830897/1‑148 (MQ=255)
                                                                                ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaa                                                                >  1:249996/1‑148 (MQ=255)
                                                                                 gAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaaa                                                               <  1:658444/148‑1 (MQ=255)
                                                                                     ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                           <  2:3366952/148‑1 (MQ=255)
                                                                                     ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                           >  1:2109297/1‑148 (MQ=255)
                                                                                     ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACCGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                           >  1:803439/1‑148 (MQ=255)
                                                                                      cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGccc                                                          <  2:3135944/148‑1 (MQ=255)
                                                                                       tGAGTTGCCGGAACGTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCt                                                         <  2:2380026/148‑1 (MQ=255)
                                                                                        gAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTg                                                        <  1:882789/148‑1 (MQ=255)
                                                                                         aGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTgg                                                       >  1:1367063/1‑148 (MQ=255)
                                                                                          gTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGt                                                      <  1:275047/148‑1 (MQ=255)
                                                                                               cGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATa                                                 <  2:146327/148‑1 (MQ=255)
                                                                                                ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                <  2:3454272/148‑1 (MQ=255)
                                                                                                ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                <  2:3455171/148‑1 (MQ=255)
                                                                                                ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                <  1:556339/148‑1 (MQ=255)
                                                                                                 gAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAc                                               >  1:2914383/1‑148 (MQ=255)
                                                                                                 gAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAc                                               >  1:465886/1‑148 (MQ=255)
                                                                                                   aCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGt                                             >  2:1540734/1‑148 (MQ=255)
                                                                                                        tATCGCAATAAAACCATTGACCCGCGCACGCAGTTTTGCCTTTAACTGTGACGGGGGAGCGAACGCGTTTTGGTGGAAAAACAGCACCTGGCGTTTACACACGGCAATCACGTAATTATTAAAAACGCCCTGGTCGATAACGTCCggg                                        <  1:3420517/148‑1 (MQ=255)
                                                                                                         ctCGCCAAAAAACCCTTGACCCTCGCCCGAAGGTTGGCCTGTAAGGGTTACTGGAGTGCGAACGGATGTTGGTGGCAAAATAGCTCGTGGGGTTTTGTGACGGAATTCAGGTCTTTATTAAAAACGCCCTGGTCGATAACGTCCgtgt                                       <  2:2667230/147‑1 (MQ=255)
                                                                                                         aTCGCCAAAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGt                                       <  1:1182719/148‑1 (MQ=255)
                                                                                                          tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtt                                      >  1:358798/1‑148 (MQ=255)
                                                                                                           cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                     >  2:3324439/1‑148 (MQ=255)
                                                                                                           cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                     >  2:3326805/1‑148 (MQ=255)
                                                                                                            gCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGGGACGGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                    <  2:110062/148‑1 (MQ=255)
                                                                                                               aTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                 >  2:2150746/1‑148 (MQ=255)
                                                                                                               aTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                 >  2:1221880/1‑148 (MQ=255)
                                                                                                               aTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                 >  1:1941744/1‑148 (MQ=255)
                                                                                                                tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                >  2:2291174/1‑148 (MQ=255)
                                                                                                                 aaaaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCGTGTTGGTGGCACATCAGCACCTGGCGGTTACTCACGGCACTCACGTCATTATGACAATCGCCCTGGTCGATAACGTCCGGGTTTTGCTgg                               >  2:914761/1‑148 (MQ=255)
                                                                                                                 aaaaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTgg                               >  1:1490920/1‑148 (MQ=255)
                                                                                                                  aaaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTggg                              >  2:2084650/1‑148 (MQ=255)
                                                                                                                    aCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGCCTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCg                            <  1:562990/148‑1 (MQ=255)
                                                                                                                     ccATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGa                           >  2:1997056/1‑148 (MQ=255)
                                                                                                                         tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGTTGGCAAAACAGAACCTGGCGGTTACTCACGGCAATCACGTAATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                       <  1:3066415/148‑1 (MQ=255)
                                                                                                                         tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                       <  2:2200786/148‑1 (MQ=255)
                                                                                                                           aCCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCa                     >  1:1374098/1‑148 (MQ=255)
                                                                                                                            cccGCGCCCGCTGGTTTGCCTTTACCTGTGACTGGCGAGCGAACGCCTGTGGGGGCCAAAACAGCACCTGGGTGTTACTCCCGCCAATCCCGACATTTTCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAc                    <  1:3411059/148‑1 (MQ=255)
                                                                                                                                cgcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt                <  1:2890079/148‑1 (MQ=255)
                                                                                                                                   acgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgg             <  2:2192337/148‑1 (MQ=255)
                                                                                                                                     gcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgggg           <  1:877647/148‑1 (MQ=255)
                                                                                                                                       tggTGTGCCTTTAACTGGGAGGGGCGCGCGACCGCTTGTGGGTGCAAAAACCGCACCTGGCGTTTACCCCCGCAAATCACGTAATTTTCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAt         <  1:2084650/147‑1 (MQ=255)
                                                                                                                                         gTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTc       <  2:116913/148‑1 (MQ=255)
                                                                                                                                         gTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTc       >  1:2733656/1‑148 (MQ=255)
                                                                                                                                         gTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTc       <  2:1662282/148‑1 (MQ=255)
                                                                                                                                          tttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCa      >  2:1511208/1‑148 (MQ=255)
                                                                                                                                           ttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAc     <  2:531255/148‑1 (MQ=255)
                                                                                                                                              ccAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCGGGCGGTTACACACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTg  <  1:1918172/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG  >  CP009273/1821552‑1821841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  CP009273/1821551‑1821843
                                                                                                                                                 |                                                                                                                                                   
AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAA                                                                                                                                                 >  SRR19973065.1526331/1‑150 (MQ=60)
 GGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC                                                                                                                                                <  SRR19973065.2482450/150‑1 (MQ=60)
      CACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGA                                                                                                                                           <  SRR19973065.2065496/150‑1 (MQ=60)
      CACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGA                                                                                                                                           <  SRR19973065.1138748/150‑1 (MQ=60)
         CAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTG                                                                                                                                        <  SRR19973065.2632475/150‑1 (MQ=60)
             ATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGA                                                                                                                                    >  SRR19973065.640609/1‑150 (MQ=60)
                   GAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAC                                                                                                                              <  SRR19973065.1765340/150‑1 (MQ=60)
                    AACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACG                                                                                                                             >  SRR19973065.1107721/1‑150 (MQ=60)
                    AACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACG                                                                                                                             >  SRR19973065.838131/1‑150 (MQ=60)
                     ACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGC                                                                                                                            >  SRR19973065.1193016/1‑150 (MQ=60)
                        ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                         >  SRR19973065.3476230/1‑150 (MQ=60)
                        ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                         >  SRR19973065.3475327/1‑150 (MQ=60)
                        ATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTG                                                                                                                         >  SRR19973065.3475299/1‑150 (MQ=60)
                         TCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGT                                                                                                                        >  SRR19973065.1458520/1‑150 (MQ=60)
                          CATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTT                                                                                                                       >  SRR19973065.1101431/1‑150 (MQ=60)
                          CATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTT                                                                                                                       >  SRR19973065.1712738/1‑150 (MQ=60)
                              GCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTG                                                                                                                   <  SRR19973065.417036/150‑1 (MQ=60)
                               CGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGG                                                                                                                  >  SRR19973065.1673415/1‑150 (MQ=60)
                                     AGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAC                                                                                                            >  SRR19973065.1062058/1‑150 (MQ=60)
                                     AGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAC                                                                                                            <  SRR19973065.1336690/150‑1 (MQ=60)
                                       CAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAG                                                                                                          <  SRR19973065.1298044/150‑1 (MQ=60)
                                           TGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACC                                                                                                      >  SRR19973065.2041718/1‑150 (MQ=60)
                                             GCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTG                                                                                                    <  SRR19973065.1181130/150‑1 (MQ=60)
                                             GCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTG                                                                                                    <  SRR19973065.1181089/150‑1 (MQ=60)
                                              CTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGG                                                                                                   <  SRR19973065.1028575/150‑1 (MQ=60)
                                              CTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGG                                                                                                   >  SRR19973065.1803320/1‑150 (MQ=60)
                                               TGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAATACAGCACCTGGC                                                                                                  >  SRR19973065.599477/1‑150 (MQ=60)
                                               TGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGC                                                                                                  >  SRR19973065.2375061/1‑150 (MQ=60)
                                               TGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGC                                                                                                  >  SRR19973065.628569/1‑150 (MQ=60)
                                                  TAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGT                                                                                               >  SRR19973065.726994/1‑150 (MQ=60)
                                                  TAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGT                                                                                               >  SRR19973065.727018/1‑150 (MQ=60)
                                                    AACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTA                                                                                             >  SRR19973065.2488122/1‑150 (MQ=60)
                                                    AACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTA                                                                                             >  SRR19973065.717934/1‑150 (MQ=60)
                                                     ACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTAC                                                                                            <  SRR19973065.3018338/150‑1 (MQ=60)
                                                       AGATAGGTAGACACCGTATCAGACACTGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTC                                                                                          >  SRR19973065.2699545/1‑150 (MQ=60)
                                                       AGATAGGTAGACACCGTATCAGACACTGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTC                                                                                          >  SRR19973065.2699522/1‑150 (MQ=60)
                                                       AGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTC                                                                                          >  SRR19973065.2318118/1‑150 (MQ=60)
                                                              TAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAA                                                                                   >  SRR19973065.1762664/1‑150 (MQ=60)
                                                               AGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAT                                                                                  <  SRR19973065.1583232/150‑1 (MQ=60)
                                                               AGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAT                                                                                  >  SRR19973065.455687/1‑150 (MQ=60)
                                                               AGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAT                                                                                  <  SRR19973065.1406012/150‑1 (MQ=60)
                                                               AGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAT                                                                                  <  SRR19973065.1405988/150‑1 (MQ=60)
                                                               AGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAT                                                                                  >  SRR19973065.2232682/1‑150 (MQ=60)
                                                                       TATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAT                                                                          >  SRR19973065.2925007/1‑150 (MQ=60)
                                                                       TATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAT                                                                          <  SRR19973065.1955561/150‑1 (MQ=60)
                                                                          CAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAT                                                                       <  SRR19973065.395553/150‑1 (MQ=60)
                                                                          CAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAT                                                                       >  SRR19973065.2884348/1‑150 (MQ=60)
                                                                           AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                      <  SRR19973065.625203/150‑1 (MQ=60)
                                                                             ACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAA                                                                    <  SRR19973065.1359633/150‑1 (MQ=60)
                                                                              CACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAA                                                                   <  SRR19973065.3482214/150‑1 (MQ=60)
                                                                               ACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAA                                                                  <  SRR19973065.960195/150‑1 (MQ=60)
                                                                               ACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAA                                                                  >  SRR19973065.251751/1‑150 (MQ=60)
                                                                               ACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAA                                                                  <  SRR19973065.1085451/150‑1 (MQ=60)
                                                                                  GAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACG                                                               <  SRR19973065.662795/150‑1 (MQ=60)
                                                                                    AACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCC                                                             >  SRR19973065.2122863/1‑150 (MQ=60)
                                                                                    AACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACCGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCC                                                             >  SRR19973065.808749/1‑150 (MQ=60)
                                                                                        TGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGG                                                         >  SRR19973065.3135636/1‑150 (MQ=60)
                                                                                        TGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGG                                                         >  SRR19973065.1376098/1‑150 (MQ=60)
                                                                                         GAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGT                                                        <  SRR19973065.888408/150‑1 (MQ=60)
                                                                                           GTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCG                                                      <  SRR19973065.276892/150‑1 (MQ=60)
                                                                                                CGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAC                                                 >  SRR19973065.469093/1‑150 (MQ=60)
                                                                                                CGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAC                                                 >  SRR19973065.2932057/1‑150 (MQ=60)
                                                                                                CGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAC                                                 >  SRR19973065.2932111/1‑150 (MQ=60)
                                                                                                 GGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACG                                                <  SRR19973065.559892/150‑1 (MQ=60)
                                                                                                 GGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACG                                                <  SRR19973065.559870/150‑1 (MQ=60)
                                                                                                         TATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTT                                        >  SRR19973065.364388/1‑150 (MQ=60)
                                                                                                         TATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTT                                        >  SRR19973065.361322/1‑150 (MQ=60)
                                                                                                         TATCGCCATAAAACCATTGACCCGCGCACGATGGTTTGCCTGTAACTGTGAGTGGCGAGCTAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGAAATCACTTAATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTT                                        <  SRR19973065.3440833/150‑1 (MQ=60)
                                                                                                         TATCGCAATAAAACCATTGACCCGCGCACGCAGTTTTGCCTTTAACTGTGACGGGGGAGCGAACGCGTTTTGGTGGAAAAACAGCACCTGGCGTTTACACACGGCAATCACGTAATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTT                                        <  SRR19973065.3441412/150‑1 (MQ=60)
                                                                                                          ATCGCCAAAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTT                                       <  SRR19973065.1190209/150‑1 (MQ=60)
                                                                                                           TCGCCATAAACCCTTTGCCCCGCGCACGCTGGTTTGCCTGTAACTGTGAGTGGCGAGCGAACGCGTGTTGGTGGAAAAACAGCACCGGGGGTTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTT                                      <  SRR19973065.1036917/150‑1 (MQ=60)
                                                                                                              CCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCT                                   >  SRR19973065.1954233/1‑150 (MQ=60)
                                                                                                              CCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCT                                   >  SRR19973065.1954442/1‑150 (MQ=60)
                                                                                                               CATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTG                                  >  SRR19973065.581678/1‑150 (MQ=60)
                                                                                                               CATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTG                                  >  SRR19973065.1180481/1‑150 (MQ=60)
                                                                                                                ATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCCCGTCCTTATGAAAAACGCCCTGGTCGATAACGTCCGGGGTTTGCTGG                                 >  SRR19973065.1500340/1‑150 (MQ=60)
                                                                                                                ATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGG                                 >  SRR19973065.1500480/1‑150 (MQ=60)
                                                                                                                   AAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCG                              >  SRR19973065.2556396/1‑150 (MQ=60)
                                                                                                                     CCCATTGACCCGCGCCCGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCGGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAA                            <  SRR19973065.566524/150‑1 (MQ=60)
                                                                                                                     ACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGCCTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAA                            <  SRR19973065.566571/150‑1 (MQ=60)
                                                                                                                         TTGACCCGCGCACGCTGGTTTGCCTTTAACTGTGACTGGCGAGCGAACGCCTGTGGGTGGAAAAACCGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATC                        <  SRR19973065.1379462/150‑1 (MQ=60)
                                                                                                                          TGCCCCGCGCACGCTGGTTTGCCTGTAACGGGGACTGGCGCGCTAACGCCTGTTGGTGGAAAAACAGCACGGGGCGGTTACTCACGGCAATCACGTAATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCA                       <  SRR19973065.1309545/150‑1 (MQ=60)
                                                                                                                          TGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCTTGTTGTTGGCAAAACAGAACCTGGCGGTTACTCACGGCAATCACGTAATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCA                       <  SRR19973065.3085159/150‑1 (MQ=60)
                                                                                                                          TGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCA                       >  SRR19973065.2196892/1‑150 (MQ=60)
                                                                                                                          TGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCA                       >  SRR19973065.1383165/1‑150 (MQ=60)
                                                                                                                             CCCGCGCCCGCTGGTTTGCCTTTACCTGTGACTGGCGAGCGAACGCCTGTGGGGGCCAAAACAGCACCTGGGTGTTACTCCCGCCAATCCCGACATTTTCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                    <  SRR19973065.3431898/150‑1 (MQ=60)
                                                                                                                             CCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGCAAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                    <  SRR19973065.285644/150‑1 (MQ=60)
                                                                                                                              CCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTG                   >  SRR19973065.1630642/1‑150 (MQ=60)
                                                                                                                                 CGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                <  SRR19973065.2908130/150‑1 (MQ=60)
                                                                                                                                 CGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                <  SRR19973065.2312888/150‑1 (MQ=60)
                                                                                                                                 CGCACGCAGGTTTGCCTGTAACGGTGACGGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTG                <  SRR19973065.2907742/150‑1 (MQ=60)
                                                                                                                                   accgaagtTTTGCCTGTAACTGTGAGTGGCGAGCGAACGCGTGTTGGTGGCAAAACCGCACGTGGCGTTTACTCACGGAAATCACGTATTTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGG              <  SRR19973065.1821826/142‑1 (MQ=60)
                                                                                                                                      GCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAT           <  SRR19973065.883246/150‑1 (MQ=60)
                                                                                                                                        AGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTC         >  SRR19973065.2750661/1‑150 (MQ=60)
                                                                                                                                        AGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTC         >  SRR19973065.2750660/1‑150 (MQ=60)
                                                                                                                                        AGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTC         >  SRR19973065.2750124/1‑150 (MQ=60)
                                                                                                                                           TTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACC      <  SRR19973065.3456077/150‑1 (MQ=60)
                                                                                                                                             TGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG    >  SRR19973065.1508202/1‑150 (MQ=60)
                                                                                                                                             TGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG    >  SRR19973065.1508219/1‑150 (MQ=60)
                                                                                                                                             TGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG    <  SRR19973065.1864162/150‑1 (MQ=60)
                                                                                                                                               CCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCGGGCGGTTACACACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  <  SRR19973065.1930754/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
AGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  CP009273/1821551‑1821843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑