Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 42.2% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT42.2% 201.2 / inf 344L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (113/85);  new base T (68/77);  total (181/163)
Fisher's exact test for biased strand distribution p-value = 6.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

Only 100 of 345 total aligned reads displayed.

CTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATTC  >  CP009273/1821548‑1821845
                                                                                                                                                    |                                                                                                                                                      
 tGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGt                                                                                                                                                      >  2:3275510/1‑150 (MQ=255)
  gAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTa                                                                                                                                                     <  1:481730/150‑1 (MQ=255)
  gAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTa                                                                                                                                                     >  2:1082484/1‑150 (MQ=255)
  gAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTa                                                                                                                                                     >  1:2897717/1‑150 (MQ=255)
  gAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTa                                                                                                                                                     <  2:476553/150‑1 (MQ=255)
   aGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTaa                                                                                                                                                    <  1:2083687/150‑1 (MQ=255)
           ccAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACt                                                                                                                                           <  2:1942772/150‑1 (MQ=255)
              aCATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGgc                                                                                                                                        <  1:4166912/150‑1 (MQ=255)
                atcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGgcga                                                                                                                                      >  2:3537246/1‑150 (MQ=255)
                atcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGgcga                                                                                                                                      >  2:1268901/1‑150 (MQ=255)
                  catGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGgcgagc                                                                                                                                    >  2:260660/1‑150 (MQ=255)
                    tGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGgcgagcga                                                                                                                                  <  2:726641/150‑1 (MQ=255)
                     ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGaa                                                                                                                                 >  1:696381/1‑150 (MQ=255)
                     ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGaa                                                                                                                                 >  1:3875719/1‑150 (MQ=255)
                     ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGaa                                                                                                                                 >  2:1465659/1‑150 (MQ=255)
                      gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAAc                                                                                                                                <  1:1897787/150‑1 (MQ=255)
                      gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAAc                                                                                                                                <  2:3024995/150‑1 (MQ=255)
                       aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACg                                                                                                                               <  1:3152370/150‑1 (MQ=255)
                       aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACg                                                                                                                               >  1:1543487/1‑150 (MQ=255)
                          catcatCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCt                                                                                                                            <  2:2018385/150‑1 (MQ=255)
                          catcatCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCt                                                                                                                            <  1:4248596/150‑1 (MQ=255)
                             catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGtt                                                                                                                         >  1:3017897/1‑150 (MQ=255)
                              atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTtg                                                                                                                        <  2:2181387/150‑1 (MQ=255)
                                cgcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTtggt                                                                                                                      >  1:3795647/1‑150 (MQ=255)
                                 gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTtggtg                                                                                                                     <  2:2226604/150‑1 (MQ=255)
                                  cgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTtggtgg                                                                                                                    >  2:3929807/1‑150 (MQ=255)
                                  cgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCAGTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTtggtgg                                                                                                                    >  2:3618897/1‑150 (MQ=255)
                                   ggCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGc                                                                                                                   >  1:23112/1‑150 (MQ=255)
                                        agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAc                                                                                                              <  1:1391811/150‑1 (MQ=255)
                                              tGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                        >  1:4731948/1‑150 (MQ=255)
                                                gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTg                                                                                                      <  1:4316322/150‑1 (MQ=255)
                                                gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTg                                                                                                      >  1:2782484/1‑150 (MQ=255)
                                                  tGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGc                                                                                                    <  2:3346681/150‑1 (MQ=255)
                                                   gTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCg                                                                                                   >  2:4184432/1‑150 (MQ=255)
                                                       aaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTa                                                                                               >  1:3335592/1‑150 (MQ=255)
                                                       aaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTa                                                                                               <  1:1300833/150‑1 (MQ=255)
                                                        aCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTAc                                                                                              >  2:2317381/1‑150 (MQ=255)
                                                           taTAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTTTTGGTGGCAAAACAGCACCTGGCGGTTACTCa                                                                                           <  2:2132159/149‑1 (MQ=255)
                                                           gATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCa                                                                                           <  2:2429072/150‑1 (MQ=255)
                                                             tAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACg                                                                                         <  1:246548/150‑1 (MQ=255)
                                                              aGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACgg                                                                                        >  1:470574/1‑150 (MQ=255)
                                                              aGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACgg                                                                                        >  1:1077825/1‑150 (MQ=255)
                                                                  aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                                    <  1:4004344/150‑1 (MQ=255)
                                                                   gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                                   <  2:3818207/150‑1 (MQ=255)
                                                                     cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCAc                                                                                 <  1:2429242/150‑1 (MQ=255)
                                                                     cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCAc                                                                                 <  1:3274592/150‑1 (MQ=255)
                                                                      acCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACg                                                                                >  2:4104697/1‑150 (MQ=255)
                                                                      acCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACg                                                                                >  1:537952/1‑150 (MQ=255)
                                                                       ccGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGt                                                                               >  2:4091900/1‑150 (MQ=255)
                                                                        cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTc                                                                              >  1:2841449/1‑150 (MQ=255)
                                                                        cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTc                                                                              >  1:4398355/1‑150 (MQ=255)
                                                                         gTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                             <  1:2200176/150‑1 (MQ=255)
                                                                         gTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                             >  1:1804707/1‑150 (MQ=255)
                                                                          tATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAt                                                                            >  1:3700095/1‑150 (MQ=255)
                                                                          tATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAt                                                                            >  2:768297/1‑150 (MQ=255)
                                                                           aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                           <  1:3122589/150‑1 (MQ=255)
                                                                            tCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTa                                                                          <  1:2323529/150‑1 (MQ=255)
                                                                             cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAt                                                                         <  2:4520486/150‑1 (MQ=255)
                                                                             cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAt                                                                         <  2:4522142/150‑1 (MQ=255)
                                                                                acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaa                                                                      >  1:1910390/1‑150 (MQ=255)
                                                                                acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaa                                                                      <  2:2372199/150‑1 (MQ=255)
                                                                                  acGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaa                                                                    >  1:120065/1‑150 (MQ=255)
                                                                                   cGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaaa                                                                   <  2:3125635/150‑1 (MQ=255)
                                                                                     gAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACg                                                                 <  1:3062516/150‑1 (MQ=255)
                                                                                       aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                               <  2:873164/150‑1 (MQ=255)
                                                                                       aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                               >  1:1489685/1‑150 (MQ=255)
                                                                                          cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTg                                                            >  2:2239216/1‑150 (MQ=255)
                                                                                                   cGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAc                                                   <  2:2675960/150‑1 (MQ=255)
                                                                                                    ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                                  <  2:4242997/150‑1 (MQ=255)
                                                                                                    ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                                  >  1:3366441/1‑150 (MQ=255)
                                                                                                       atttatATCGCAATAAAACAATTGCCCCGCGCACGCTGTTTTGCCTTTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACACACGGAAATCACTTATTTATCAAAAACGCCCTGGTCGATAACGTcc                                               <  1:3222402/145‑1 (MQ=255)
                                                                                                       aCTTCTATCGCAATAAAACCATTGACCCGCGCACGCTGGTTTGCCTTTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGGGTTTACTCACGGAAATCACTTCATTATTAAAAACGCCCTGGTCGATAACGTcc                                               <  1:967946/150‑1 (MQ=255)
                                                                                                        cTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                              <  2:2929571/150‑1 (MQ=255)
                                                                                                        cTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                              >  1:4582015/1‑150 (MQ=255)
                                                                                                        attatATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTTTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                              <  1:3921923/146‑1 (MQ=255)
                                                                                                           cTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGt                                           >  2:4013262/1‑150 (MQ=255)
                                                                                                              tCGCCATAACACCATTGACCCGCGCCCGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                        <  2:114156/150‑1 (MQ=255)
                                                                                                              tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                        <  2:524182/150‑1 (MQ=255)
                                                                                                               cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTg                                       >  1:349966/1‑150 (MQ=255)
                                                                                                                  cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                    >  2:3503042/1‑150 (MQ=255)
                                                                                                                  cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                    >  2:1729740/1‑150 (MQ=255)
                                                                                                                   aTAAAACCTTTGACCCGCGCACGCTGTTTTGCC‑TTAACGTGTGACTGGCGAGCTAACGCGTGTTGGTGCAAAAACAGCACCTGGGGTTTACTCACGGAAATCACTTATTTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTgg                                   <  1:1648193/150‑1 (MQ=255)
                                                                                                                    tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTggg                                  >  2:3193490/1‑150 (MQ=255)
                                                                                                                      aaaCCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCg                                >  2:4748844/1‑150 (MQ=255)
                                                                                                                      aaaCCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCg                                >  1:4073265/1‑150 (MQ=255)
                                                                                                                       aaCCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGa                               >  2:2298890/1‑150 (MQ=255)
                                                                                                                           atggACCCGCGCACGCCGGTTTGCCATTACC‑TGGGACGGGCGAGCTACCGCCTGTTGGTGGCAAAACCGCACCGGGCGGTTACTCACGCCAACCACGACATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                           <  1:4535410/147‑1 (MQ=255)
                                                                                                                            ttGACCCGCGCACGCAGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATc                          <  2:4639363/150‑1 (MQ=255)
                                                                                                                            ttGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGTGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATc                          <  2:3170324/150‑1 (MQ=255)
                                                                                                                                   gcgcCCGCTGGTTTGCCAGTAAC‑TGTGACTGGCGTGCTAACGCCTGTTGGTGGAAAAACAGCACCGGGCGGTTACTCCCGGCAATCACTTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt                   <  1:105344/150‑1 (MQ=255)
                                                                                                                                   gcgcACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt                   >  1:2642544/1‑150 (MQ=255)
                                                                                                                                    cgcacgcaGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTg                  >  2:1410628/1‑150 (MQ=255)
                                                                                                                                     gcacgcaGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgg                 >  1:1626636/1‑150 (MQ=255)
                                                                                                                                       ccgctGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgggg               <  1:2581960/149‑1 (MQ=255)
                                                                                                                                        cgcaGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGa              <  2:1579290/150‑1 (MQ=255)
                                                                                                                                           aGGTTTGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTc           >  1:4307417/1‑150 (MQ=255)
                                                                                                                                            ggTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCa          >  2:2784786/1‑150 (MQ=255)
                                                                                                                                                tGCCTGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTg      >  2:3411740/1‑150 (MQ=255)
                                                                                                                                                 gCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGa     >  2:576547/1‑150 (MQ=255)
                                                                                                                                                  ccAGTAAC‑AGTGACTGGCGAGCGAACGCCTGTAGGTGGCAAAACACCACCTGGCGGTTACTCACGGCACTCACGTCAATATGAAAAACGCCCAGGTCGATAAACTCCCGGTTTTGCTGGGCGAAAATCACATGATGGGGATTCACCTGAt    >  2:656379/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAAC‑TGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATTC  >  CP009273/1821548‑1821845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A