Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 100% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT99.3% 605.4 / ‑4.4 184L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (83/99);  total (84/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

GCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCT  >  CP009273/1821553‑1821840
                                                                                                                                               |                                                                                                                                                
gcagcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTaa                                                                                                                                              <  1:1423008/148‑1 (MQ=255)
 cagcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAc                                                                                                                                             <  1:1162525/148‑1 (MQ=255)
    caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                          >  2:525958/1‑148 (MQ=255)
      ccAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCGTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGa                                                                                                                                        <  2:1240341/148‑1 (MQ=255)
      ccAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGa                                                                                                                                        <  1:2616632/148‑1 (MQ=255)
        aaCATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACt                                                                                                                                      <  1:2405768/148‑1 (MQ=255)
          catcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTgg                                                                                                                                    >  1:3098833/1‑148 (MQ=255)
           atcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgc                                                                                                                                   <  2:1172172/148‑1 (MQ=255)
            tcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcg                                                                                                                                  <  2:301073/148‑1 (MQ=255)
               tGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagc                                                                                                                               >  2:2555816/1‑148 (MQ=255)
                ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagcg                                                                                                                              >  2:96301/1‑148 (MQ=255)
                 gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagcga                                                                                                                             <  1:3342364/148‑1 (MQ=255)
                  aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGaa                                                                                                                            <  2:2126123/148‑1 (MQ=255)
                     catcatCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGc                                                                                                                         <  1:1295362/148‑1 (MQ=255)
                     catcatCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGc                                                                                                                         <  1:2988470/148‑1 (MQ=255)
                      atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGcc                                                                                                                        >  1:1002435/1‑148 (MQ=255)
                       tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCt                                                                                                                       >  2:664081/1‑148 (MQ=255)
                        catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                      <  1:769186/148‑1 (MQ=255)
                          tcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                    <  2:272685/148‑1 (MQ=255)
                             cgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTtggt                                                                                                                 >  1:1241378/1‑148 (MQ=255)
                               gCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggtgg                                                                                                               <  2:292874/148‑1 (MQ=255)
                               gCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggtgg                                                                                                               <  1:871660/148‑1 (MQ=255)
                                   agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaaa                                                                                                           <  1:2290670/148‑1 (MQ=255)
                                   agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaaa                                                                                                           <  1:305736/148‑1 (MQ=255)
                                    gcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaaaa                                                                                                          >  1:2029887/1‑148 (MQ=255)
                                        ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGc                                                                                                      <  1:2462798/148‑1 (MQ=255)
                                         tGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCa                                                                                                     <  2:2770692/148‑1 (MQ=255)
                                          ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAc                                                                                                    <  1:133690/148‑1 (MQ=255)
                                          ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAc                                                                                                    <  1:511409/148‑1 (MQ=255)
                                            cTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                  <  2:2177627/148‑1 (MQ=255)
                                            cTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                  <  1:3344194/148‑1 (MQ=255)
                                              gTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTgg                                                                                                >  2:2080097/1‑148 (MQ=255)
                                               ttAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGc                                                                                               >  2:2732574/1‑148 (MQ=255)
                                                    cAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTa                                                                                          <  2:2199779/148‑1 (MQ=255)
                                                    cAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTa                                                                                          >  2:1891058/1‑148 (MQ=255)
                                                    cAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTa                                                                                          <  2:2200768/148‑1 (MQ=255)
                                                      gATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACt                                                                                        >  1:3376723/1‑148 (MQ=255)
                                                           gTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACgg                                                                                   >  2:3814895/1‑148 (MQ=255)
                                                               acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                               <  1:620150/148‑1 (MQ=255)
                                                                cacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                              <  1:908132/148‑1 (MQ=255)
                                                                     tATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTc                                                                         <  2:1603026/148‑1 (MQ=255)
                                                                      aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCa                                                                        <  1:1035714/148‑1 (MQ=255)
                                                                        cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                      >  2:1693549/1‑148 (MQ=255)
                                                                           acacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                   >  1:3698163/1‑148 (MQ=255)
                                                                             acGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaa                                                                 >  2:1578372/1‑148 (MQ=255)
                                                                                  aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACg                                                            <  1:1543378/148‑1 (MQ=255)
                                                                                   aCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGc                                                           >  1:2302605/1‑148 (MQ=255)
                                                                                    ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                          <  1:2862703/148‑1 (MQ=255)
                                                                                    ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                          <  1:288715/148‑1 (MQ=255)
                                                                                    ccTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                          >  1:1928169/1‑148 (MQ=255)
                                                                                     cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGccc                                                         >  2:566127/1‑148 (MQ=255)
                                                                                      tGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCt                                                        >  2:401855/1‑148 (MQ=255)
                                                                                          ttGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTc                                                    >  1:1369538/1‑148 (MQ=255)
                                                                                          ttGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTc                                                    >  1:3457690/1‑148 (MQ=255)
                                                                                            gCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGa                                                  <  2:3308312/148‑1 (MQ=255)
                                                                                            gCCGGAACTTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTAATTATGAAAAACGCCCTGGTCGa                                                  <  1:2184849/148‑1 (MQ=255)
                                                                                             ccGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAt                                                 >  1:1048071/1‑148 (MQ=255)
                                                                                                  aCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGt                                            <  2:1234830/148‑1 (MQ=255)
                                                                                                     tCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                         >  1:3268346/1‑148 (MQ=255)
                                                                                                       tATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCggg                                       <  2:1369538/148‑1 (MQ=255)
                                                                                                          cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGCCTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                    <  2:1483870/148‑1 (MQ=255)
                                                                                                          cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAACCAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                    <  2:1483342/148‑1 (MQ=255)
                                                                                                          cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                    >  2:628420/1‑148 (MQ=255)
                                                                                                            ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTg                                  <  2:1241425/148‑1 (MQ=255)
                                                                                                            ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACGGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTg                                  <  2:2310010/148‑1 (MQ=255)
                                                                                                             cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGc                                 <  2:2829698/148‑1 (MQ=255)
                                                                                                             cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGc                                 >  1:71897/1‑148 (MQ=255)
                                                                                                             cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCGGTTGGTGGCAAAACCGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGc                                 <  2:1083127/148‑1 (MQ=255)
                                                                                                              aTAAAACCATTGACCCGCGCACGAAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTAGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                <  2:1712865/148‑1 (MQ=255)
                                                                                                               tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                               <  2:1329019/148‑1 (MQ=255)
                                                                                                               tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                               <  2:2305723/148‑1 (MQ=255)
                                                                                                               tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTAATCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                               <  2:1329142/148‑1 (MQ=255)
                                                                                                               tAAAACCATTCACCCGCGCCCGCTGGTTTGCCAGTAAGTGTGAGTGGCGAGCGAACGCGTGTTGGTGGAAAAACAGCACCGGGGGTTAACTCACGGCAATCACGTATTTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                               <  1:1791144/148‑1 (MQ=255)
                                                                                                                aaaaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTgg                              >  1:3220032/1‑148 (MQ=255)
                                                                                                                  aaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGc                            >  2:3299906/1‑148 (MQ=255)
                                                                                                                  aaCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGc                            >  1:187076/1‑148 (MQ=255)
                                                                                                                      attcccccGCCCACGCAGTTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCCCGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaa                        <  1:96301/143‑1 (MQ=255)
                                                                                                                       ttGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                       <  1:186969/148‑1 (MQ=255)
                                                                                                                       ttGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                       >  1:40186/1‑148 (MQ=255)
                                                                                                                       ttGACCCGCGCACGCAGGTTTGCCTGTAACGGGGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                       <  2:2996594/148‑1 (MQ=255)
                                                                                                                        tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                      >  2:1763263/1‑148 (MQ=255)
                                                                                                                        tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                      >  2:1763412/1‑148 (MQ=255)
                                                                                                                        tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                      >  2:3654546/1‑148 (MQ=255)
                                                                                                                          aCCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCa                    <  2:3257592/148‑1 (MQ=255)
                                                                                                                           cccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGCCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAc                   <  1:2746642/148‑1 (MQ=255)
                                                                                                                            ccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAcc                  >  1:2144517/1‑148 (MQ=255)
                                                                                                                            ccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGAACCTGGCGGTTAATCACGGCAATCACTACATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAcc                  <  1:2554574/148‑1 (MQ=255)
                                                                                                                             cgcgCCCGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCt                 <  1:3668537/148‑1 (MQ=255)
                                                                                                                                gcacgctGGTTTGCCTTTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACGTGGCGGTTACACACGGAAATCACGTCTTTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGtt              <  1:3638992/148‑1 (MQ=255)
                                                                                                                                 cacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTg             <  2:2241965/148‑1 (MQ=255)
                                                                                                                                   cgcaGGTTT‑CCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgggg          <  2:787962/148‑1 (MQ=255)
                                                                                                                                     caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGa         >  2:516544/1‑148 (MQ=255)
                                                                                                                                     caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGa         >  1:1466224/1‑148 (MQ=255)
                                                                                                                                     caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGa         >  2:2440531/1‑148 (MQ=255)
                                                                                                                                       ggTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt       <  2:3098833/148‑1 (MQ=255)
                                                                                                                                         tttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCa     >  2:3736682/1‑148 (MQ=255)
                                                                                                                                          ttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAc    >  1:3490684/1‑148 (MQ=255)
                                                                                                                                           tGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAcc   <  2:3496108/148‑1 (MQ=255)
                                                                                                                                           tGCCTGTAACTGTGACTGGCGAGCGAACCCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAcc   <  2:2607006/148‑1 (MQ=255)
                                                                                                                                            gCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCt  >  2:3214020/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCT  >  CP009273/1821553‑1821840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  CP009273/1821547‑1821843
                                                                                                                                                     |                                                                                                                                                   
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCT                                                                                                                                                     <  SRR19973069.2336269/150‑1 (MQ=60)
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCT                                                                                                                                                     <  SRR19973069.800531/150‑1 (MQ=60)
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCT                                                                                                                                                     >  SRR19973069.1506228/1‑150 (MQ=60)
   GAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTA                                                                                                                                                  <  SRR19973069.787894/150‑1 (MQ=60)
      GCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACT                                                                                                                                               <  SRR19973069.1432952/150‑1 (MQ=60)
       CAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTG                                                                                                                                              <  SRR19973069.3089016/150‑1 (MQ=60)
       CAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTG                                                                                                                                              <  SRR19973069.1170604/150‑1 (MQ=60)
            CCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACT                                                                                                                                         <  SRR19973069.2634127/150‑1 (MQ=60)
              AACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGG                                                                                                                                       <  SRR19973069.1676615/150‑1 (MQ=60)
              AACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGG                                                                                                                                       >  SRR19973069.3119248/1‑150 (MQ=60)
              AACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGG                                                                                                                                       <  SRR19973069.2422040/150‑1 (MQ=60)
                 ATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGA                                                                                                                                    >  SRR19973069.1857004/1‑150 (MQ=60)
                       GAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAC                                                                                                                              <  SRR19973069.3556625/150‑1 (MQ=60)
                       GAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAC                                                                                                                              <  SRR19973069.3364244/150‑1 (MQ=60)
                       GAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAC                                                                                                                              <  SRR19973069.3364298/150‑1 (MQ=60)
                        AACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACG                                                                                                                             <  SRR19973069.3224770/150‑1 (MQ=60)
                          CCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCC                                                                                                                           >  SRR19973069.1009313/1‑150 (MQ=60)
                           CATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCT                                                                                                                          <  SRR19973069.3008318/150‑1 (MQ=60)
                           CATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCT                                                                                                                          <  SRR19973069.1304076/150‑1 (MQ=60)
                           CATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCT                                                                                                                          >  SRR19973069.3391857/1‑150 (MQ=60)
                              CCTCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTT                                                                                                                       <  SRR19973069.1970426/150‑1 (MQ=60)
                              CATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTT                                                                                                                       <  SRR19973069.774623/150‑1 (MQ=60)
                                 CGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGT                                                                                                                    >  SRR19973069.1249816/1‑150 (MQ=60)
                                     GCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCA                                                                                                                <  SRR19973069.877721/150‑1 (MQ=60)
                                        CAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA                                                                                                             >  SRR19973069.2043993/1‑150 (MQ=60)
                                        CAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAA                                                                                                             >  SRR19973069.2043989/1‑150 (MQ=60)
                                         AGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAC                                                                                                            <  SRR19973069.307796/150‑1 (MQ=60)
                                         AGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAC                                                                                                            <  SRR19973069.2306234/150‑1 (MQ=60)
                                             GTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCA                                                                                                        <  SRR19973069.24503/150‑1 (MQ=60)
                                              TTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAC                                                                                                       <  SRR19973069.2479331/150‑1 (MQ=60)
                                               TGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACC                                                                                                      <  SRR19973069.1144299/150‑1 (MQ=60)
                                                GGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCT                                                                                                     <  SRR19973069.514922/150‑1 (MQ=60)
                                                GGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCT                                                                                                     <  SRR19973069.515046/150‑1 (MQ=60)
                                                GGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCT                                                                                                     <  SRR19973069.134934/150‑1 (MQ=60)
                                                  CTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGG                                                                                                   <  SRR19973069.3366086/150‑1 (MQ=60)
                                                          CAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACT                                                                                           >  SRR19973069.3398796/1‑150 (MQ=60)
                                                          CAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACT                                                                                           >  SRR19973069.1422978/1‑150 (MQ=60)
                                                           AGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTC                                                                                          >  SRR19973069.1937733/1‑150 (MQ=60)
                                                               AGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGG                                                                                      >  SRR19973069.1249863/1‑150 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCA                                                                                <  SRR19973069.624274/150‑1 (MQ=60)
                                                                     ACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCA                                                                                <  SRR19973069.2319789/150‑1 (MQ=60)
                                                                      CACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCAC                                                                               <  SRR19973069.914355/150‑1 (MQ=60)
                                                                         CGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTT‑CCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCA                                                                           >  SRR19973069.793555/1‑150 (MQ=60)
                                                                            ATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATT                                                                         <  SRR19973069.1042904/150‑1 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                      >  SRR19973069.3722163/1‑150 (MQ=60)
                                                                               AGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATG                                                                      >  SRR19973069.3722333/1‑150 (MQ=60)
                                                                                      GAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACG                                                               <  SRR19973069.899649/150‑1 (MQ=60)
                                                                                       AAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGC                                                              >  SRR19973069.2318268/1‑150 (MQ=60)
                                                                                       AAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGC                                                              >  SRR19973069.380423/1‑150 (MQ=60)
                                                                                        AACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCC                                                             >  SRR19973069.1941413/1‑150 (MQ=60)
                                                                                        AACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCC                                                             <  SRR19973069.1553949/150‑1 (MQ=60)
                                                                                          CCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCT                                                           <  SRR19973069.2881522/150‑1 (MQ=60)
                                                                                          CCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCT                                                           <  SRR19973069.290708/150‑1 (MQ=60)
                                                                                           CTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTG                                                          <  SRR19973069.3510883/150‑1 (MQ=60)
                                                                                           CTGAGTTGCCGGAACTTATATCGCAATAAAACCATTGACCCGCGCACGCAGTTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTG                                                          <  SRR19973069.2283894/150‑1 (MQ=60)
                                                                                              AGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTC                                                       >  SRR19973069.1379066/1‑150 (MQ=60)
                                                                                              AGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTC                                                       >  SRR19973069.3480082/1‑150 (MQ=60)
                                                                                                 TGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAT                                                    >  SRR19973069.1052856/1‑150 (MQ=60)
                                                                                                 TGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAT                                                    >  SRR19973069.1055345/1‑150 (MQ=60)
                                                                                                  GCCGGAACTTCTATCGCAATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTAATTATGAAAAACGCCCTGGTCGATA                                                   <  SRR19973069.2199730/150‑1 (MQ=60)
                                                                                                    CGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAC                                                 <  SRR19973069.963363/150‑1 (MQ=60)
                                                                                                        ACTTCTATCGCCATAAAACCATTGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCC                                             <  SRR19973069.641280/150‑1 (MQ=60)
                                                                                                         CTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCG                                            >  SRR19973069.3289754/1‑150 (MQ=60)
                                                                                                               TCGCCATAAAACCATTGACGCGCGCAGGCAGGTTGGCGTGTAACTGTGTCGGGGGTGCTACCGCGTGTTGGTGGCAAAACTGCACCGGGGGGTTACTCCCGCCAATCACGTCATTATCAAAAACGCCGTGGTCGATAACGTCCGGGTTTT                                      <  SRR19973069.2084017/150‑1 (MQ=45)
                                                                                                               TCGCAAAAAAACCATTGACCCGCCCACGCTGGTTTGCCTTTAACTGTGACTGGCGAGCGACCGCGTGTTGGTGCAAAAACAGCACCGGGGGTTTACTCACGCAAATCACGTATTTTTCAAAAACGCCCTGGTCGATAACGTCCGGGTTTT                                      <  SRR19973069.846676/150‑1 (MQ=50)
                                                                                                                 GCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGC                                    >  SRR19973069.72710/1‑150 (MQ=60)
                                                                                                                   CATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTG                                  >  SRR19973069.3044081/1‑150 (MQ=60)
                                                                                                                    ATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGG                                 >  SRR19973069.3241062/1‑150 (MQ=60)
                                                                                                                    ATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGG                                 >  SRR19973069.3241241/1‑150 (MQ=60)
                                                                                                                     TAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGG                                >  SRR19973069.3432935/1‑150 (MQ=60)
                                                                                                                     TAAAACCATTCACCCGCGCCCGCTGGTTTGCCAGTAAGTGTGAGTGGCGAGCGAACGCGTGTTGGTGGAAAAACAGCACCGGGGGTTAACTCACGGCAATCACGTATTTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGG                                <  SRR19973069.1803763/150‑1 (MQ=60)
                                                                                                                      AAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGC                               >  SRR19973069.188617/1‑150 (MQ=60)
                                                                                                                       AAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCG                              >  SRR19973069.2571107/1‑150 (MQ=60)
                                                                                                                        AACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGA                             >  SRR19973069.1327893/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                          >  SRR19973069.1245470/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                          >  SRR19973069.2919107/1‑150 (MQ=60)
                                                                                                                           CATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAA                          >  SRR19973069.40714/1‑150 (MQ=60)
                                                                                                                            attccCCCGCCCACGCAGTTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCCCGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAT                         <  SRR19973069.97280/145‑1 (MQ=60)
                                                                                                                             TTGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATC                        <  SRR19973069.188508/150‑1 (MQ=60)
                                                                                                                              TGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGTTTACACACGGAAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCA                       <  SRR19973069.529680/150‑1 (MQ=60)
                                                                                                                                ACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACC                     >  SRR19973069.2159244/1‑150 (MQ=60)
                                                                                                                                ACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACC                     >  SRR19973069.2159236/1‑150 (MQ=60)
                                                                                                                                 CCCGCGCACGCAGGTTTGCGTTTAACTGTGACTGGCGCGCGAACGCCTTTTGGTGTAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTAATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                    <  SRR19973069.2765010/150‑1 (MQ=60)
                                                                                                                                 CCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                    <  SRR19973069.2764492/150‑1 (MQ=60)
                                                                                                                                 CCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGCCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCT                    <  SRR19973069.2764995/150‑1 (MQ=60)
                                                                                                                                  CCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGAACCTGGCGGTTAATCACGGCAATCACTACATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTG                   <  SRR19973069.2571447/150‑1 (MQ=60)
                                                                                                                                  CCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTG                   <  SRR19973069.2572694/150‑1 (MQ=60)
                                                                                                                                   CGCGCCCGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGAAATCACGTCATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGT                  <  SRR19973069.3692548/150‑1 (MQ=60)
                                                                                                                                      GCACGCTGGTTTGCCTTTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACGTGGCGGTTACACACGGAAATCACGTCTTTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGG               <  SRR19973069.3662823/150‑1 (MQ=60)
                                                                                                                                         CGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGA            >  SRR19973069.1476491/1‑150 (MQ=60)
                                                                                                                                         CGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGA            >  SRR19973069.1476456/1‑150 (MQ=60)
                                                                                                                                         CGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGA            >  SRR19973069.1476349/1‑150 (MQ=60)
                                                                                                                                              GTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAC       >  SRR19973069.3513196/1‑150 (MQ=60)
                                                                                                                                                 TGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG    >  SRR19973069.3751698/1‑150 (MQ=60)
                                                                                                                                                  GCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGA   >  SRR19973069.1666453/1‑150 (MQ=60)
                                                                                                                                                   CCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  SRR19973069.2025603/1‑150 (MQ=60)
                                                                                                                                                   CCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  SRR19973069.2295379/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAT  >  CP009273/1821547‑1821843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑