Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA95.9% 593.3 / 14.6 197A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base A (114/75);  total (120/77)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG  >  CP009273/2404629‑2404915
                                                                                                                                                |                                                                                                                                               
tCGCCTAAAATAAACCTGGCGCCAATCAGGACACTTTTTGTTCCATAAACGCCGTAAATTAGTTGGGGTTGTTTAAGGGTTCCGTTTAAT‑CAAACGAAAAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaa                                                                                                                                             <  2:51163/148‑1 (MQ=255)
  gCCAATAATAAACAAGGCGGCAATCAGGAAAACTTTAGTATAACAAAAGCCGTAAAATACTTCTGGTAGTGTCAGCGTTCCGCTTAATCCAACCG‑CTACAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAAc                                                                                                                                           <  2:2075328/148‑1 (MQ=255)
    cAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                         >  1:3479799/1‑148 (MQ=255)
    cAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                         >  2:2852704/1‑148 (MQ=255)
     aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAg                                                                                                                                        >  1:1100685/1‑148 (MQ=255)
     aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAg                                                                                                                                        >  1:3177860/1‑148 (MQ=255)
      ttaataaACAAGGCCACATACAGAACAACTTTAGTATAATAAACGCCGAAAATTATTTCTGGGACTTTCTGCGTTCCGTTTATTGAAAACG‑CCAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGa                                                                                                                                       <  2:2541145/147‑1 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                      >  2:456052/1‑148 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                      >  2:1223182/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                   >  1:512236/1‑148 (MQ=255)
              cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCa                                                                                                                               >  2:2661835/1‑148 (MQ=255)
              cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCa                                                                                                                               >  2:2148298/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCAAACGCACTCTGCACGAAGCAAACAAGACTATGGCaa                                                                                                                              >  1:2466112/1‑148 (MQ=255)
                 ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                            >  1:2144332/1‑148 (MQ=255)
                  gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACgg                                                                                                                           >  1:1561987/1‑148 (MQ=255)
                    ggaaaTCAGGACACCTTTAGTAAAAAAAACGCCGTAAAAAACTTCGGGTAGTGAAAGGTTCCCGCTTAATCCAACCG‑CTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGcc                                                                                                                         <  2:3380215/145‑1 (MQ=255)
                       aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGt                                                                                                                      >  2:1531505/1‑148 (MQ=255)
                       aaTCAGGACAACGTTAGGATAATAAAAGCCGTAAAATACTTCGGGTACTGTCAGCGTTCCGCTTAATGCAACCG‑CTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGt                                                                                                                      <  2:1390681/148‑1 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:1001061/1‑148 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:2441612/1‑148 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:1469191/1‑148 (MQ=255)
                          cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                   >  2:3344078/1‑148 (MQ=255)
                          cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                   >  1:1053463/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:3530556/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:2902981/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:1074970/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:2288120/1‑148 (MQ=255)
                                       gtaTCAAAAAAGCGTTAAATTACTTGTGGGACTGTCTGCTTTCCGATTAATGCAACCG‑CGAAAATAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      <  2:833203/146‑1 (MQ=255)
                                       ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      >  2:842440/1‑148 (MQ=255)
                                       ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      >  1:1281675/1‑148 (MQ=255)
                                        taTCATAAAAGCCTAAAATTACTTGTGGCTTTGTCAGTGTTCCTTTTATTTAAACCG‑CCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                                                     <  1:2809578/147‑1 (MQ=255)
                                         aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc                                                                                                    >  2:3444359/1‑148 (MQ=255)
                                           cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  >  1:2170764/1‑148 (MQ=255)
                                           aataacaGCCGCAAAATATTTTGGGTAGTGTCGGGTTTCCTCTTAATCAAAACG‑CTAAAAAAAAAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  <  2:985417/142‑1 (MQ=255)
                                           aataaaaGCGGTAAAATACTTCTGGGACTGTCTGCGTTCCGCTTAATGAAAACG‑CCAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  <  2:2606634/145‑1 (MQ=255)
                                             gAAAAGCCGTAAAATACTTCTGGCAGTGTAAGGTTTCCTATTATTGCAACCG‑GCAAAATAACAAACACAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                <  2:72487/148‑1 (MQ=255)
                                             caaaaGCCTTAAAATACTTGTGGTAGTGTATGCGTACGGTATAATCAAAACG‑CAAAAAAAAAAAACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                <  1:1915268/147‑1 (MQ=255)
                                               aaaGCCGTAAAATACTTCTGGGACTTTCTGCGTTCCGATTAATCCAAACG‑CTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                              <  2:2305420/148‑1 (MQ=255)
                                               aaaGCCGCAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGCAAACG‑CGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                              <  2:1886563/148‑1 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                             >  2:1940323/1‑148 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                             >  1:2933925/1‑148 (MQ=255)
                                                   ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                          >  1:681816/1‑148 (MQ=255)
                                                   ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                          >  1:1885042/1‑148 (MQ=255)
                                                     ttaaaaTACTTGTGGTACTTTCTGCTTTCCTCTTAATCAAAACG‑CAAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                        <  1:1794877/146‑1 (MQ=255)
                                                       aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                                                      >  1:300828/1‑148 (MQ=255)
                                                         attattgcTGGGACTGTCAGCGTTCCTATTAATGAAAACG‑CGAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:3076195/141‑1 (MQ=255)
                                                         aaTACTTCTGGTACTTTCAGCGTTCCGCTTAATGCAACCG‑CGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:2370664/148‑1 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    >  1:1760020/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    >  1:2070408/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAACCG‑CGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:2370489/148‑1 (MQ=255)
                                                           tACTTTTGGTACTGTAAGCGTTCCTCTTAATCCAAACG‑CGAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                  <  2:3143573/148‑1 (MQ=255)
                                                           tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                  >  1:555533/1‑148 (MQ=255)
                                                             cTTCTGGGACTGTATGCGTTCCGATTAATGAAAACG‑CTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                                                                                <  2:2778470/148‑1 (MQ=255)
                                                              ttgtGGTACTGTCAGCGTTCCTCTAAATCCAACCG‑ATAAAAAAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  1:2251560/145‑1 (MQ=255)
                                                              ttgtGGGTCTTTCTGGGTTCCGCTTAATGCAACCG‑CCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  1:888630/145‑1 (MQ=255)
                                                              ttCTGGGACTGTCAGCTTTCCGATTAATGAAACCG‑CTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  2:556101/148‑1 (MQ=255)
                                                                   ggACTGTCAGGTTTCCTTTTAATTAAACCG‑GTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                          <  2:464161/148‑1 (MQ=255)
                                                                   ggACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                          >  1:2205407/1‑148 (MQ=255)
                                                                     aCTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                                                        >  2:1543310/1‑148 (MQ=255)
                                                                      gtGTCAGCGTTCCGTTTAATGCAACCG‑CTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                       <  2:3067828/147‑1 (MQ=255)
                                                                        gTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAt                                                                     >  2:1055151/1‑148 (MQ=255)
                                                                         tCAGCGTTCCGCTTAATGCACACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                                                    >  1:1940655/1‑148 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                   >  1:3136336/1‑148 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                   >  1:1932892/1‑148 (MQ=255)
                                                                           aGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                                                  >  2:1685626/1‑148 (MQ=255)
                                                                                tccttttAATCAAAAAG‑AGAAAATAAAAAAAACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             <  1:2361341/143‑1 (MQ=255)
                                                                                tCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             >  1:1901017/1‑148 (MQ=255)
                                                                                tCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             >  2:1895948/1‑148 (MQ=255)
                                                                                 ccGCTTAATGCAAACG‑CGAAAAAAACAAAAAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                                            <  2:3177860/148‑1 (MQ=255)
                                                                                    cTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                         >  1:2920986/1‑148 (MQ=255)
                                                                                    attAATGCAACCG‑CCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                         <  1:3247950/147‑1 (MQ=255)
                                                                                         tGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                    >  1:3179650/1‑148 (MQ=255)
                                                                                          gCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTAATCCTTGTTAATTGAGGGGGATGACTTGAt                                                   >  2:355218/1‑148 (MQ=255)
                                                                                          gCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                   >  2:356735/1‑148 (MQ=255)
                                                                                           cAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                  >  2:3287919/1‑148 (MQ=255)
                                                                                                cgctaaaaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                                              <  2:1846804/142‑1 (MQ=255)
                                                                                                  cgACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                                            >  1:3306082/1‑148 (MQ=255)
                                                                                                    aCAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATa                                          >  1:2188027/1‑148 (MQ=255)
                                                                                                     cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                         >  1:2517755/1‑148 (MQ=255)
                                                                                                     cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                         >  1:2570143/1‑148 (MQ=255)
                                                                                                       aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                       >  1:2512239/1‑148 (MQ=255)
                                                                                                            aacccccgCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                  <  1:2429650/141‑1 (MQ=255)
                                                                                                             aacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                 <  1:2661829/148‑1 (MQ=255)
                                                                                                                  aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                            <  2:2282021/148‑1 (MQ=255)
                                                                                                                    cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                          <  2:1799917/148‑1 (MQ=255)
                                                                                                                    cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                          <  2:53269/148‑1 (MQ=255)
                                                                                                                       ccGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                       >  1:1666631/1‑148 (MQ=255)
                                                                                                                         gCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAAAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                     <  1:3356961/148‑1 (MQ=255)
                                                                                                                             ccATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                 >  2:570058/1‑148 (MQ=255)
                                                                                                                               aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTcaa               >  2:3139798/1‑148 (MQ=255)
                                                                                                                               aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa               >  1:80539/1‑148 (MQ=255)
                                                                                                                                  cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa            <  1:258643/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCTATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCAATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:877100/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:1264184/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:876760/148‑1 (MQ=255)
                                                                                                                                     aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa         <  2:1100685/148‑1 (MQ=255)
                                                                                                                                      ctctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt        >  1:2666553/1‑148 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  2:928505/148‑1 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  2:3479799/148‑1 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAACAATTCACCGTTCACAACTTATCATTGTTAATTGTGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  1:1531505/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG  >  CP009273/2404629‑2404915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCA  >  CP009273/2404623‑2404920
                                                                                                                                                     |                                                                                                                                                    
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                      >  SRR19973067.3341418/1‑150 (MQ=60)
 aacaatcccaaataaaaaacccggcgcaaatcagtacaacttttgtaaaacaaaacccttaaaatccttgtggtactttccgcttccctattaatcaacaccccaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAG                                                                                                                                                     <  SRR19973067.2359788/41‑1 (MQ=60)
 AACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAG                                                                                                                                                     >  SRR19973067.2623570/1‑150 (MQ=60)
        GCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAA                                                                                                                                              >  SRR19973067.3501886/1‑150 (MQ=60)
         aaaaaaaaaaacctggcttaataaagaaaatgtttctaataaaaaacgcccaattttttttggtgttctttcttgtttactataatcaaaaactaaaaaaaaacaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAG                                                                                                                                             <  SRR19973067.1517220/39‑1 (MQ=60)
         CCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAG                                                                                                                                             >  SRR19973067.1108173/1‑150 (MQ=60)
         CCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAG                                                                                                                                             >  SRR19973067.3197968/1‑150 (MQ=60)
          aaataaaaaacccggcggaaatccgcacaacgttcttaacacaaacgccttaaaatacttcggggactgtctgcgttccgattaatcaaaccgccaaaaaaaaaaccaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                            <  SRR19973067.3554108/40‑1 (MQ=60)
              aaaacccctggggcaaatcctaaaaactttttaaacaaaaaaccccaaaaatatttgtgttagttccgggttccctttaaaccaaaccgcaaaaaaaaaaaccaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAT                                                                                                                                        <  SRR19973067.1834894/44‑1 (MQ=60)
              aaaacccctcgctcaaatccccaaaacttatttaaaaaaaaagcctaaaattttttgtgttagtgtcaggtttcttataaaccaaaccccaaaaaaaacaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAT                                                                                                                                        <  SRR19973067.1834911/44‑1 (MQ=60)
              AATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAT                                                                                                                                        >  SRR19973067.515723/1‑150 (MQ=60)
                 aaccccggcgcaattctgtacattttttgtatcaaaaacgcctaaaaatagttgttggagtgtgcgggtgccttttaatcaaacccaaaaAATAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                     <  SRR19973067.1050084/60‑1 (MQ=60)
                   ACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAAACGCACTCTGCACGAAGCAAACAAGACTATGGCAA                                                                                                                                   >  SRR19973067.2482332/1‑150 (MQ=60)
                    ttgtttttcctttcttaaaatttttgaaacataaaaggcttaattttattgtgtttgtttagggtttctttttatttaaaaccataaaaaaaaaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                  <  SRR19973067.1714959/50‑1 (MQ=60)
                     AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                                 >  SRR19973067.2158663/1‑150 (MQ=60)
                      gggggcaattccgtaaatgtttattataacaaatgcattaatttatgtgtgtgtgtgtctggtttccttttaatcaacacgataaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGG                                                                                                                                <  SRR19973067.394919/52‑1 (MQ=60)
                      AGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGG                                                                                                                                >  SRR19973067.1572662/1‑150 (MQ=60)
                           caattcgctaaccttttgtttaacaaaacgcgttaaatatttttgggccttctcggctttaatataatcaaaaacctaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGT                                                                                                                           <  SRR19973067.1628633/57‑1 (MQ=60)
                           caatcatttaaaattttattaaaaaaaccctttaatttttttttgggtttttgctgtttacctataaataaaacacaaaaaaaaaaaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGT                                                                                                                           <  SRR19973067.1569014/57‑1 (MQ=60)
                           acaatctgcacaatttttttaaataaaactcctaaatttagttgtggctttgtctgttttcctttaaataaacacccaaaaaaaacaacaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGT                                                                                                                           <  SRR19973067.1628624/57‑1 (MQ=60)
                              ATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAT                                                                                                                        >  SRR19973067.1060685/1‑150 (MQ=60)
                               caatcaaatttttctataaaaaatcgcctaaaattttttttgtctttttctgtttacctttaaacaaaaacacaaaaaaaacaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATG                                                                                                                       <  SRR19973067.1520594/61‑1 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                     >  SRR19973067.1944382/1‑150 (MQ=60)
                                   aaatattttataataataaaagccttaaatttcttgtggcattttcaggtttccgtttaaccaaaccgccACAATAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                   <  SRR19973067.1087514/80‑1 (MQ=60)
                                   GACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                   >  SRR19973067.3235860/1‑150 (MQ=60)
                                      tagtttttcaacaaaaacgcctcaaaatagttgggggtgtgtctgctgcgcgataaataaaaccccaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAC                                                                                                                <  SRR19973067.2540917/68‑1 (MQ=60)
                                        cttttttataaaaaacgccttaaaatatttgtggcactgtctgggtccggtttaatcaaacccccaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCC                                                                                                              <  SRR19973067.863147/70‑1 (MQ=60)
                                        CGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCC                                                                                                              >  SRR19973067.2771552/1‑150 (MQ=60)
                                         tttctcaaaataaacccgttaaattctttgggggtggtacggggtccctataaacccaaccccaaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                             <  SRR19973067.729210/71‑1 (MQ=60)
                                         ttaattaacacaaatgatataatttttttgttgttgtgtcggttttcttaattatcaaaaccaaaaaaaaaaaaaaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                             <  SRR19973067.3299926/71‑1 (MQ=60)
                                         tataataacaaaacttccaaaatattctgttgtctttgtctggtttcctataaaaaaaacccacaaaaaaacaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                             <  SRR19973067.1526011/71‑1 (MQ=60)
                                           TAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAA                                                                                                           >  SRR19973067.1290406/1‑150 (MQ=60)
                                            attaaaaaaaaaccctaaaattatttgtgttattgtatgggtcccgattaataaaaaccccaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                          <  SRR19973067.1849519/74‑1 (MQ=60)
                                              tatcataaaagcctaaaattacttgtggctttgtcagtgttccttttatttaaaccgccaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCA                                                                                                        <  SRR19973067.2827688/76‑1 (MQ=60)
                                              taaaataaacgcgttaaaatttttttggttgtgtcgggtttccgataattcaaaccgctaaaaaaacaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCA                                                                                                        <  SRR19973067.2827699/76‑1 (MQ=60)
                                               ATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAA                                                                                                       >  SRR19973067.2185282/1‑150 (MQ=60)
                                                acagaaatgcattaatattttggtgtgtgtttctgagttcggtttattgaaaacgcaaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAT                                                                                                      <  SRR19973067.1993539/91‑1 (MQ=60)
                                                aaaaaaatccttaaaattatttgtggttttttctgggttccttttaatcaaaccccaaaaaaacaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAT                                                                                                      <  SRR19973067.124592/85‑1 (MQ=60)
                                                 CAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATT                                                                                                     >  SRR19973067.2255834/1‑150 (MQ=60)
                                                  aaaaaagcgttaaaatatttgggggagtgtgtggtttgcgtttattcaaaacgataaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTC                                                                                                    <  SRR19973067.1409870/87‑1 (MQ=60)
                                                  aaaaaagcctaaaaatatttgtggtatttagtggtttcatattaatcaaaaccaaaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTC                                                                                                    <  SRR19973067.425544/87‑1 (MQ=60)
                                                   caaaagccttaaaatacttgtggtagtgtatgcgtacggtataatcaaaacgcaaaaaaaaaaaaccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                   <  SRR19973067.1928341/81‑1 (MQ=60)
                                                   aaaatgcgttaaatttgttttggttttttcaggtttctttttattccaaagtcaaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                   <  SRR19973067.1927904/88‑1 (MQ=60)
                                                   aaaatccttaaaaatttttttgttgtgttatgttttcacttactccaaaaccaaaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                   <  SRR19973067.310736/81‑1 (MQ=60)
                                                    AAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAC                                                                                                  >  SRR19973067.2497637/1‑150 (MQ=60)
                                                    AAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAC                                                                                                  >  SRR19973067.2952546/1‑150 (MQ=60)
                                                    AAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAC                                                                                                  >  SRR19973067.2952705/1‑150 (MQ=60)
                                                     AAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACCATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAAC                                                                                                 >  SRR19973067.3095329/1‑150 (MQ=60)
                                                       AGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                               >  SRR19973067.686503/1‑150 (MQ=60)
                                                       AGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                               >  SRR19973067.2374663/1‑150 (MQ=60)
                                                       AGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                               >  SRR19973067.2481665/1‑150 (MQ=60)
                                                       AGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                               >  SRR19973067.1897909/1‑150 (MQ=60)
                                                           ttaaaatacttgtggtactttctgctttcctcttaatcaaaacgcaaaAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAC                                                                                           <  SRR19973067.1806954/102‑1 (MQ=60)
                                                           GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAC                                                                                           >  SRR19973067.302751/1‑150 (MQ=60)
                                                             AAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAA                                                                                         >  SRR19973067.1772003/1‑150 (MQ=60)
                                                             AAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAA                                                                                         >  SRR19973067.2084163/1‑150 (MQ=60)
                                                              aattatttgggttactgtctgcgttccttttaatccaaacgataaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAAC                                                                                        <  SRR19973067.3497760/99‑1 (MQ=60)
                                                               attacttctgggattgactgggtgccgctaaaccaaaacccaaaaaaacaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                       <  SRR19973067.2605351/100‑1 (MQ=60)
                                                               AATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                       >  SRR19973067.559181/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                      >  SRR19973067.1774294/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                      >  SRR19973067.3163526/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                      >  SRR19973067.1919668/1‑150 (MQ=60)
                                                                  atttgtggtacttcatgggttcctattaatccaaacgctaaaaaaacaaccaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                    <  SRR19973067.3255282/96‑1 (MQ=60)
                                                                  atttgtgggagttcctggtttccgattaatcaaaacccaaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                    <  SRR19973067.203694/103‑1 (MQ=60)
                                                                  ACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                    >  SRR19973067.934781/1‑150 (MQ=60)
                                                                    ttgtttcagtgtccggtttctttttaatccaaacccgaaaaaaacaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCC                                                                                  <  SRR19973067.2177960/98‑1 (MQ=60)
                                                                    ttgtggtagtgtcagcgttcctattaatccaaccccaaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCC                                                                                  <  SRR19973067.2266431/98‑1 (MQ=60)
                                                                    ttgtggtactgtcagcgttcctctaaatccaaccgataaaaaaaaAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCC                                                                                  <  SRR19973067.2266508/105‑1 (MQ=60)
                                                                    ttgtgggtctttctggGTTCCGCTTAATGCAACCGCCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCC                                                                                  <  SRR19973067.894523/134‑1 (MQ=60)
                                                                      ggggtagtttccgcttcccgataattccaaccccaaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                                <  SRR19973067.3410827/100‑1 (MQ=60)
                                                                      ctggtggggcccgcttccataaaaatcaaaaccaaaaaaaaaaaccccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                                <  SRR19973067.909156/100‑1 (MQ=60)
                                                                      CTGGGACTGTCAGCGTTCCGATTAATGCAACCGCTAAAAAAACAAACCAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                                <  SRR19973067.2941332/150‑1 (MQ=60)
                                                                       tgtcagttcagggtgtcctattaatcaaaacccaaaaaaacaaccacacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                               <  SRR19973067.2121705/101‑1 (MQ=60)
                                                                       tgggagggtctcgttaccgataaaaaaacccaaaaaaaaaccaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                               <  SRR19973067.2121956/101‑1 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                               >  SRR19973067.2220115/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                               >  SRR19973067.2220106/1‑150 (MQ=60)
                                                                         ggagtttccgcgtcccgtttaaccaaaccccaacaaaaaaAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTT                                                                             <  SRR19973067.473045/110‑1 (MQ=60)
                                                                           ACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAA                                                                           >  SRR19973067.1697583/1‑150 (MQ=60)
                                                                            ttttctgcgtcccttaaaagcaaaacgaaaaaaaacaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAT                                                                          <  SRR19973067.1588086/113‑1 (MQ=60)
                                                                            ctttccggtttcctattaaacaaaacccaaaaaaacaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAT                                                                          <  SRR19973067.1588096/113‑1 (MQ=60)
                                                                            ctgccaggttacatataacccaaaacccaaaaaaaaaaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAT                                                                          <  SRR19973067.1610619/106‑1 (MQ=60)
                                                                             TGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATT                                                                         >  SRR19973067.1953888/1‑150 (MQ=60)
                                                                             TGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATT                                                                         >  SRR19973067.1953910/1‑150 (MQ=60)
                                                                             TGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAGT                                                                         >  SRR19973067.1953626/1‑150 (MQ=60)
                                                                              gtccgggttccgattaatcaaaccgccaaaaaaccaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTG                                                                        <  SRR19973067.1913207/108‑1 (MQ=60)
                                                                              GTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTG                                                                        >  SRR19973067.1946077/1‑150 (MQ=60)
                                                                              GTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTG                                                                        >  SRR19973067.3156255/1‑150 (MQ=60)
                                                                              GTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTG                                                                        >  SRR19973067.136504/1‑150 (MQ=60)
                                                                                caggttacccataaatccaaccccaaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAG                                                                      <  SRR19973067.2921800/117‑1 (MQ=60)
                                                                                  ggtttctgtttaacccaaccgaaaaaaaaacaacaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTTATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGG                                                                    <  SRR19973067.460944/112‑1 (MQ=60)
                                                                                  gggtcccaaaaaacaaaaacccaaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGG                                                                    <  SRR19973067.460952/112‑1 (MQ=60)
                                                                                  gcttacgtattactaaaaaccaaaaaaaaacaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGG                                                                    <  SRR19973067.659834/112‑1 (MQ=60)
                                                                                   cttaccgattaaacaaaacgacaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGG                                                                   <  SRR19973067.1113411/113‑1 (MQ=60)
                                                                                    GTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGG                                                                  >  SRR19973067.1913977/1‑150 (MQ=60)
                                                                                      tccttttaatcaaaaagagaaaataaaaaaaacAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                                <  SRR19973067.2376730/117‑1 (MQ=60)
                                                                                       CCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATG                                                               >  SRR19973067.1602588/1‑150 (MQ=60)
                                                                                        cgattaatccaaacgctaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGA                                                              <  SRR19973067.569644/125‑1 (MQ=60)
                                                                                        CGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGA                                                              >  SRR19973067.2939561/1‑150 (MQ=60)
                                                                                          ataaaccaaacccccaaaaaaccaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                            <  SRR19973067.1056805/120‑1 (MQ=60)
                                                                                          ataaaccaaacccaaaaaaaaacaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                            <  SRR19973067.2720560/120‑1 (MQ=60)
                                                                                          ataaacaaaacccacaaaaaaaaaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                            <  SRR19973067.2719163/120‑1 (MQ=60)
                                                                                          ATTAATGCAACCGCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                            <  SRR19973067.3268283/150‑1 (MQ=60)
                                                                                            aaatcaaaaccacaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTG                                                          <  SRR19973067.1067656/122‑1 (MQ=60)
                                                                                             aaacaaacacccaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGA                                                         <  SRR19973067.1256729/123‑1 (MQ=60)
                                                                                             AATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGA                                                         >  SRR19973067.3199772/1‑150 (MQ=60)
                                                                                              ctccaaaccacacaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                        <  SRR19973067.2796243/124‑1 (MQ=60)
                                                                                              atcaaccccaaaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                        <  SRR19973067.3427995/131‑1 (MQ=60)
                                                                                              accaaaaccccaaaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                        <  SRR19973067.2796286/131‑1 (MQ=60)
                                                                                                 caaaccaaacaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT                                                     <  SRR19973067.3134635/127‑1 (MQ=60)
                                                                                                 CAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAGTCT                                                     >  SRR19973067.2599828/1‑150 (MQ=60)
                                                                                                     cgacaaaaaaacaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCNA                                                 <  SRR19973067.2765578/131‑1 (MQ=60)
                                                                                                     CGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCA                                                 >  SRR19973067.3326940/1‑150 (MQ=60)
                                                                                                       CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATA                                               >  SRR19973067.2202639/1‑150 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAA                                              >  SRR19973067.3299111/1‑150 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAA                                              >  SRR19973067.2534263/1‑150 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAA                                              >  SRR19973067.1857883/1‑150 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAA                                              >  SRR19973067.2586914/1‑150 (MQ=60)
                                                                                                         ACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAA                                             >  SRR19973067.474820/1‑150 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                            >  SRR19973067.2528723/1‑150 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                            >  SRR19973067.2529341/1‑150 (MQ=60)
                                                                                                                 acacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                     <  SRR19973067.2162661/143‑1 (MQ=60)
                                                                                                                 aacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                     <  SRR19973067.2445596/143‑1 (MQ=60)
                                                                                                                 AAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                     >  SRR19973067.2122965/1‑150 (MQ=60)
                                                                                                                  AACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                    <  SRR19973067.2678950/150‑1 (MQ=60)
                                                                                                                  AACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                    <  SRR19973067.2678956/150‑1 (MQ=60)
                                                                                                                   ACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTGAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGA                                   >  SRR19973067.549710/1‑150 (MQ=60)
                                                                                                                    CAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGAC                                  >  SRR19973067.2195013/1‑150 (MQ=60)
                                                                                                                     accGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                                 <  SRR19973067.2568463/147‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACTATGGCAACGGCCAGTAATGAAAGAACCCAAAACAATACACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACCAGACG                            >  SRR19973067.3448365/1‑150 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                            >  SRR19973067.1678126/1‑150 (MQ=60)
                                                                                                                              GCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGC                        <  SRR19973067.3378238/150‑1 (MQ=60)
                                                                                                                              GCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAAAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGC                        <  SRR19973067.3378269/150‑1 (MQ=60)
                                                                                                                                ATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTA                      >  SRR19973067.765758/1‑150 (MQ=60)
                                                                                                                                 TCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAA                     >  SRR19973067.2877824/1‑150 (MQ=60)
                                                                                                                                  CCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAA                    >  SRR19973067.81432/1‑150 (MQ=60)
                                                                                                                                   CATACGCACTCTGCCCGTAGCAAACAAGACGATGCCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAT                   <  SRR19973067.85057/150‑1 (MQ=60)
                                                                                                                                    ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCATTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                  <  SRR19973067.1551394/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG               <  SRR19973067.260413/150‑1 (MQ=60)
                                                                                                                                         CACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAT             >  SRR19973067.2683682/1‑150 (MQ=60)
                                                                                                                                            TCTGCCCGTAGCAAACAAGCCGATGGCAACGGCCAGTAATGAAAGAACCCAAAACAATTCACCGTTCACAACTTATCATTGTTAATTGTGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAG          <  SRR19973067.1541853/150‑1 (MQ=60)
                                                                                                                                                CCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT      <  SRR19973067.2422691/150‑1 (MQ=60)
                                                                                                                                                 CCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTT     <  SRR19973067.1231333/150‑1 (MQ=60)
                                                                                                                                                    TAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCA  <  SRR19973067.459409/150‑1 (MQ=60)
                                                                                                                                                    TAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCA  <  SRR19973067.459207/150‑1 (MQ=60)
                                                                                                                                                    TAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCA  <  SRR19973067.3127932/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCA  >  CP009273/2404623‑2404920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑