Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,404,772 C→A A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA99.6% 829.9 / NA 249A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (128/120);  total (129/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404625‑2404918
                                                                                                                                                   |                                                                                                                                                  
  cATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaa                                                                                                                                                >  1:1877271/1‑150 (MQ=255)
   aTCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaaa                                                                                                                                               >  2:3128110/1‑150 (MQ=255)
   aTCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaaa                                                                                                                                               >  1:2416201/1‑150 (MQ=255)
    tctcaaataatAAACATGGTGGAAATCTGTACATTGTTTGTATATTAAATGCCGTAAAATATTTTTGGTATTGTCAGTGTTCCGTTTAATGAAAACGCTAAAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAAc                                                                                                                                              <  1:1159486/145‑1 (MQ=255)
     cGCCAATAATAAACAAGGCGGAAATCAGGAAAACTTTAGTATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACa                                                                                                                                             <  2:471747/150‑1 (MQ=255)
      gcaaataatAAACATGGCGGCAATCTGGACAACTTTAGTATAAGAAAAGCCGTAAAATACTTCTGGGACTGTCTGCGTTCCGATTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                            <  2:1038576/147‑1 (MQ=255)
      gCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                                                                                                            >  1:100748/1‑150 (MQ=255)
      gCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                            <  2:2852834/150‑1 (MQ=255)
         aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                         <  2:3357981/150‑1 (MQ=255)
         aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                         >  1:1582295/1‑150 (MQ=255)
          agaagcaaCCAGCCGGCAATCAGGACAACGTTAGGATCAGAAATGCCGTAAAATCCTCCTGGGACTGTCTGCGTTCCGCTTAATGCAAACGCGACAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACTAGACg                                                                                                                                        <  2:2509179/144‑1 (MQ=255)
           taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                       >  2:115037/1‑150 (MQ=255)
           taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                       >  1:5160/1‑150 (MQ=255)
            aataaACAAGGCGGCAATCAGGACAACGTTTGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                      <  2:3077905/150‑1 (MQ=255)
             ataaACATGGCGTCAATCTGGAAAATTTTTTTATCATAAAAGCCTTAAAATTTTTTTGGGATTGTCTGTGTTCCGTTTATTTAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATg                                                                                                                                     <  1:1361581/150‑1 (MQ=255)
                  cAAGGCGGCAATCAGTACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                                <  2:624276/150‑1 (MQ=255)
                  aaaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                                <  2:1576581/149‑1 (MQ=255)
                   aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAca                                                                                                                               <  2:2936978/150‑2 (MQ=255)
                        ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAg                                                                                                                          >  2:487641/1‑150 (MQ=255)
                        ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAg                                                                                                                          >  2:2971215/1‑150 (MQ=255)
                            ataagtaCAATGTTAGTATCATAAAAGCTTTAAAATATTTTTGGTATTGTCTGTGTTCCGTTTAATTAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                      <  1:3595442/144‑1 (MQ=255)
                            aTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                      >  2:25595/1‑150 (MQ=255)
                             tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                                                     >  2:2078065/1‑150 (MQ=255)
                               aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                   >  1:192392/1‑150 (MQ=255)
                                 gACAACGTTAGTATCATAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                                 <  2:833317/150‑1 (MQ=255)
                                    aaCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAc                                                                                                              >  2:1917893/1‑150 (MQ=255)
                                     aCGTTAGTATCATAAAAGCTGTAAAATACTTCTGGGATTGTCTGCGTTCCGATTAATGAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                                                                                                             <  1:2483108/150‑1 (MQ=255)
                                       gTTAGTATCATAAAAGCCGTAAAATATTTCTGGTATTGTCTGGGTTCCGATTAATTAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                           <  1:880693/150‑1 (MQ=255)
                                       gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                           >  1:257434/1‑150 (MQ=255)
                                        ttAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                                                                          >  2:1952105/1‑150 (MQ=255)
                                           ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc                                                                                                       <  2:3334835/150‑1 (MQ=255)
                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                      >  1:3076478/1‑150 (MQ=255)
                                             aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                     <  2:76196/150‑1 (MQ=255)
                                              tCAGAAAAGCTGTAAAATACTTCTGGGATTGTCTGCGTTCCGATTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                                                    <  1:3105100/150‑1 (MQ=255)
                                               cAGAAAAGCCGGACAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                   >  2:24574/1‑150 (MQ=255)
                                                aGAAAAGCCGTAAATTACTTCTGGTACTGTCAGCGTTCCGCTTAATGAAAACGCGACAATAACAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                  <  2:3495104/150‑1 (MQ=255)
                                                aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                  >  2:1372376/1‑150 (MQ=255)
                                                 taaaaGCTGTAAAATATTTTTGGGACTGTCAGCGTTCCGTTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                                 <  1:2159965/149‑1 (MQ=255)
                                                 gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                                 >  1:3743839/1‑150 (MQ=255)
                                                 gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                                 <  2:1472880/150‑1 (MQ=255)
                                                 gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                                 >  1:1374889/1‑150 (MQ=255)
                                                  aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                                <  2:876810/150‑1 (MQ=255)
                                                   aaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACACGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                               >  1:3455027/1‑150 (MQ=255)
                                                    aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                              >  2:1818363/1‑150 (MQ=255)
                                                      gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATCAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                                            <  2:59487/150‑1 (MQ=255)
                                                        cGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                                                          >  2:846924/1‑150 (MQ=255)
                                                        cGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                                                          >  1:1782196/1‑150 (MQ=255)
                                                          gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                                                        >  2:1740538/1‑150 (MQ=255)
                                                           aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                       >  2:778416/1‑150 (MQ=255)
                                                             aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                     >  1:2666402/1‑150 (MQ=255)
                                                             aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                     >  2:2843294/1‑150 (MQ=255)
                                                             aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                     >  1:2864620/1‑150 (MQ=255)
                                                              aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                    >  1:1997136/1‑150 (MQ=255)
                                                                aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                                  >  2:2441313/1‑150 (MQ=255)
                                                                 cTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                                                                 >  1:593086/1‑150 (MQ=255)
                                                                   tCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCt                                                                               <  2:3317486/150‑1 (MQ=255)
                                                                     tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                             <  2:3587847/150‑1 (MQ=255)
                                                                     tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                             >  1:3134663/1‑150 (MQ=255)
                                                                      gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                                                                            >  2:3643481/1‑150 (MQ=255)
                                                                      gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                                                                            >  1:775958/1‑150 (MQ=255)
                                                                        gACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                          >  1:3647014/1‑150 (MQ=255)
                                                                        gACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                          <  2:1202110/150‑1 (MQ=255)
                                                                           tGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                                                       <  2:3235051/150‑1 (MQ=255)
                                                                              cAGTGTTCCGATTAATTCAAACGCTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                                                                    <  1:1047987/150‑1 (MQ=255)
                                                                                    tCCGCTTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt                                                              <  1:2443584/150‑1 (MQ=255)
                                                                                    tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt                                                              >  2:441609/1‑150 (MQ=255)
                                                                                     ccGCTTAATGCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                             <  2:817630/150‑1 (MQ=255)
                                                                                     ccGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                             <  2:36896/150‑1 (MQ=255)
                                                                                       gCTTAATGCAAACGCGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                                                           >  2:2729773/1‑150 (MQ=255)
                                                                                       gCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                                                           >  2:3248043/1‑150 (MQ=255)
                                                                                          tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTg                                                        >  1:3290504/1‑150 (MQ=255)
                                                                                           aaTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCATTGAGGGGGATGACTTGa                                                       >  2:2627841/1‑150 (MQ=255)
                                                                                               cAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCt                                                   <  2:3450000/150‑1 (MQ=255)
                                                                                                aaaCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCt                                                   >  1:572615/1‑149 (MQ=255)
                                                                                                     cgACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATa                                             >  1:1393147/1‑150 (MQ=255)
                                                                                                       aCAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaa                                           >  1:2963529/1‑150 (MQ=255)
                                                                                                          aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaaa                                        >  2:2987943/1‑150 (MQ=255)
                                                                                                             acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                     <  1:2121327/150‑1 (MQ=255)
                                                                                                               aaacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcccc                                   >  1:2557249/1‑150 (MQ=255)
                                                                                                                aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg                                  <  1:1395958/150‑1 (MQ=255)
                                                                                                                aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg                                  <  2:2416201/150‑1 (MQ=255)
                                                                                                                     aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                             <  2:3108687/150‑1 (MQ=255)
                                                                                                                     aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                             <  1:1076581/150‑1 (MQ=255)
                                                                                                                      gCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                            <  1:2304204/150‑1 (MQ=255)
                                                                                                                       cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                           >  2:1045018/1‑150 (MQ=255)
                                                                                                                         aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                         >  2:1514158/1‑150 (MQ=255)
                                                                                                                         aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTGGTTAATTGAGGGGGATGACTTGATTCTGCCATCAAAAAGCCCCGACGAGACgg                         >  2:2529218/1‑150 (MQ=255)
                                                                                                                             cATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCt                     >  2:3158955/1‑150 (MQ=255)
                                                                                                                               tCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaa                   >  1:3019271/1‑150 (MQ=255)
                                                                                                                                 cATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAt                 >  1:793708/1‑150 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                <  1:429587/150‑1 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                <  2:2648741/150‑1 (MQ=255)
                                                                                                                                   tACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa               <  2:3630096/150‑1 (MQ=255)
                                                                                                                                    aCGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAt              <  1:3144284/150‑1 (MQ=255)
                                                                                                                                       cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt           <  2:247549/150‑1 (MQ=255)
                                                                                                                                          tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAg        <  2:2877400/150‑1 (MQ=255)
                                                                                                                                           ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg       <  1:3536543/150‑1 (MQ=255)
                                                                                                                                               ccGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt   >  2:1875691/1‑150 (MQ=255)
                                                                                                                                               ccGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt   <  2:2125777/150‑1 (MQ=255)
                                                                                                                                                cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGttt  <  1:487641/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404625‑2404918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTC  >  CP009273/2404623‑2404919
                                                                                                                                                     |                                                                                                                                                   
aaaccatctaaaataataaacatggtggcaatctggaaaatgtttgtataataaatgccgtaaaatattttTGGGACTGTCAGCGTTCCGTTTAATGCAAACGCTACAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                     <  SRR19973061.1433529/79‑1 (MQ=60)
aaaccatcgcaaataataaaaatggggtaaatctgtaaaatttttgtattataaatgccgtaaaatatttttggtattgtctgtGTTCCGTTTAATGAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                     <  SRR19973061.1230225/66‑1 (MQ=47)
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                     >  SRR19973061.3597045/1‑150 (MQ=60)
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                     >  SRR19973061.846392/1‑150 (MQ=60)
  ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGC                                                                                                                                                   >  SRR19973061.1387216/1‑150 (MQ=60)
    CATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAA                                                                                                                                                 >  SRR19973061.1881942/1‑150 (MQ=60)
     ATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAA                                                                                                                                                >  SRR19973061.512173/1‑150 (MQ=60)
     ATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAA                                                                                                                                                >  SRR19973061.2422369/1‑150 (MQ=60)
      tctcaaataataaacatggtggaaatctgtacattgtttgtatattaaatgccgtaaaatatttttggtatTGTCAGTGTTCCGTTTAATGAAAACGCTAAAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAAC                                                                                                                                               <  SRR19973061.1162423/79‑1 (MQ=37)
        GCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAA                                                                                                                                             >  SRR19973061.101080/1‑150 (MQ=60)
         caaataataaacatggcggaaatctgtacaatgtttgtataataaatgccgtaaaatattttTGGGATTGTCTGCGTTCCGTTTATTGCAAACGCTACAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAG                                                                                                                                            <  SRR19973061.1090433/88‑1 (MQ=54)
          CAATAATAAACATGGCGGCAATAAGTACAACGTTTGTATCATAAAAGCCGTAAAATATTTTTGGTATTGTCTGGGTTCCGATTAATGAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                           <  SRR19973061.1270417/150‑1 (MQ=60)
          CAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                           >  SRR19973061.1373362/1‑150 (MQ=60)
          CAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                           >  SRR19973061.1373447/1‑150 (MQ=60)
          CAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                           >  SRR19973061.2312140/1‑150 (MQ=60)
          CAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGA                                                                                                                                           >  SRR19973061.402335/1‑150 (MQ=60)
           aataataaacatggcgtcaatctgtacaatttttgtataataaatgctgtaaaatatttttgggtttttctgtGTTCCGATTAATTCAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAC                                                                                                                                          <  SRR19973061.534799/77‑1 (MQ=58)
           AATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAC                                                                                                                                          >  SRR19973061.1586224/1‑150 (MQ=60)
             TAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGA                                                                                                                                        >  SRR19973061.5177/1‑150 (MQ=60)
               ataaacatggcgtcaatctggaaaatttttttatcataaaagccttaaaatttttttgggattgtctgtgttccgtttatttaaaacgctaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATG                                                                                                                                      <  SRR19973061.1365016/58‑1 (MQ=60)
               ataaaaatggtgtaaatatttaaaatttttttataataaaagctttaaaatttttttggtattttctgtgttccgtttatttaaaacgttaaaaaaaaaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATG                                                                                                                                      <  SRR19973061.758924/45‑1 (MQ=60)
               aaaaacatggggtaaatatgtaaaatgtttgtataataaatgctgtaaattatttgtggttttttctgtgttccgtttatttaaaactctaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATG                                                                                                                                      <  SRR19973061.1942675/58‑1 (MQ=60)
                 aaaaatggtgtaaatatttaaaatttttttataataaatgctgtaaaatatttttggtattgtttggtttccttttaattaaatcgctaaAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                    <  SRR19973061.3521352/60‑1 (MQ=60)
                       GGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGC                                                                                                                              >  SRR19973061.793587/1‑150 (MQ=60)
                        GCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCC                                                                                                                             >  SRR19973061.2851020/1‑150 (MQ=60)
                             AATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                        >  SRR19973061.3620466/1‑150 (MQ=60)
                              ataattaaaatttttttatattaaaatcttaaaaaatttttgttttttgtttgttttctgttaatttaaaactttaaaaaaaaAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAT                                                                                                                       <  SRR19973061.398178/67‑1 (MQ=60)
                              ataagtacaatgttagtatcataaaagctttaaaatatttttggtattgtctgtgttccgtttaattaaaacgctaaAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAT                                                                                                                       <  SRR19973061.3604654/73‑1 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                    >  SRR19973061.192971/1‑150 (MQ=60)
                                      aatgtttttataataaattctttaaaatatttgtggtattgtctgtgttccttttaatccaaactctaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAC                                                                                                               <  SRR19973061.3223144/81‑1 (MQ=60)
                                      AATGTTAGTATCATAAAAGCCGGAAAATACTTTTGGGACTGTCAGCGTTCCGATTATTGAAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAC                                                                                                               <  SRR19973061.3453895/150‑1 (MQ=60)
                                       ACGTTAGTATCATAAAAGCTGTAAAATACTTCTGGGATTGTCTGCGTTCCGATTAATGAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACC                                                                                                              <  SRR19973061.2489428/150‑1 (MQ=60)
                                         gtttgtataatAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGTTTAATGCAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                            <  SRR19973061.1850874/139‑1 (MQ=60)
                                         GTTAGTATCATAAAAGCTGTAAAATACTTTTGGGATTGTCTGCGTTCCGTTTATTTAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                            <  SRR19973061.882911/150‑1 (MQ=60)
                                         GTTAGTATCATAAAAGCCGTAAAATATTTCTGGTATTGTCTGGGTTCCGATTAATTAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                            <  SRR19973061.882979/150‑1 (MQ=60)
                                         GTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGATTGTCTGCGTTCCGATTAATTCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                            <  SRR19973061.3750818/150‑1 (MQ=60)
                                         GTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                            >  SRR19973061.258141/1‑150 (MQ=60)
                                             GTATCAGAAAAGCTGTAAAATATTTTTGGGATTGTCAGCGTTCCGATTAATGCAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATC                                                                                                        <  SRR19973061.1560128/150‑1 (MQ=60)
                                              GATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCA                                                                                                       >  SRR19973061.3084414/1‑150 (MQ=60)
                                                taatAAAAGCCGTAAAATAATTCTGGGATTGTCTGTGTTCCGTTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAT                                                                                                     <  SRR19973061.3089474/146‑1 (MQ=60)
                                                TCAGAAATGCCGGAAAATACTTCTGGGACTGTCTGCGTTCCGATTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAT                                                                                                     <  SRR19973061.3113114/150‑1 (MQ=60)
                                                TCAGAAAAGCTGTAAAATACTTCTGGGATTGTCTGCGTTCCGATTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAT                                                                                                     <  SRR19973061.3113101/150‑1 (MQ=60)
                                                   taaaagctgtaaaatattttTGGGACTGTCAGCGTTCCGTTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                  <  SRR19973061.2165389/130‑1 (MQ=60)
                                                   GAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                  >  SRR19973061.1378363/1‑150 (MQ=60)
                                                   GAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCA                                                                                                  >  SRR19973061.3753420/1‑150 (MQ=60)
                                                     AAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACC                                                                                                >  SRR19973061.3639378/1‑150 (MQ=60)
                                                     AAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACACGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACC                                                                                                >  SRR19973061.3463891/1‑150 (MQ=60)
                                                      AAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                               >  SRR19973061.1827272/1‑150 (MQ=60)
                                                         CCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTC                                                                                            >  SRR19973061.2131114/1‑150 (MQ=60)
                                                          tttaaaatatttttggttttgtatgtgttctgtttaattaaaactctaaaataaaaaaaAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA                                                                                           <  SRR19973061.2335385/91‑1 (MQ=60)
                                                          cgtaaattatttttggttttgtttgtgttctgtttatttaaaacgctaaAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA                                                                                           <  SRR19973061.104643/101‑1 (MQ=60)
                                                          CGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA                                                                                           >  SRR19973061.1786661/1‑150 (MQ=60)
                                                          CGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA                                                                                           >  SRR19973061.3577294/1‑150 (MQ=60)
                                                               AATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                      >  SRR19973061.2925186/1‑150 (MQ=60)
                                                               AATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                      >  SRR19973061.2871959/1‑150 (MQ=60)
                                                               AATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                      >  SRR19973061.2673259/1‑150 (MQ=60)
                                                               AATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACT                                                                                      >  SRR19973061.2049879/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                     >  SRR19973061.42724/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                     >  SRR19973061.2002138/1‑150 (MQ=60)
                                                                  ACTTTTGGGACTGTCAGTGTTCCGTTTAATGCAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                   <  SRR19973061.3055235/150‑1 (MQ=60)
                                                                   CTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATC                                                                                  >  SRR19973061.594633/1‑150 (MQ=60)
                                                                    ttttgggattgtctgtgttcctaTTAATGAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCC                                                                                 <  SRR19973061.3513303/127‑1 (MQ=60)
                                                                      ttgggattgtctgtgttcagattaatgaaaacgttaaAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                               <  SRR19973061.2877182/113‑1 (MQ=60)
                                                                      ctggtattgtctgttttctgattatttaaaacgctaaAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                               <  SRR19973061.1657009/113‑1 (MQ=60)
                                                                      CTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                               >  SRR19973061.1091062/1‑150 (MQ=60)
                                                                      CTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTT                                                                               >  SRR19973061.3539898/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                              >  SRR19973061.3142910/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                              >  SRR19973061.3142735/1‑150 (MQ=60)
                                                                        GGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGT                                                                             >  SRR19973061.777998/1‑150 (MQ=60)
                                                                          gattgtctgtgttccttTTAATGAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTA                                                                           <  SRR19973061.506465/133‑1 (MQ=60)
                                                                          GACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTA                                                                           >  SRR19973061.3656331/1‑150 (MQ=60)
                                                                           ACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAA                                                                          >  SRR19973061.2465394/1‑150 (MQ=60)
                                                                             TGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATT                                                                        >  SRR19973061.2495668/1‑150 (MQ=60)
                                                                                CAGTGTTCCGATTAATTCAAACGCTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAG                                                                     <  SRR19973061.1050672/150‑1 (MQ=60)
                                                                                 AGCGTTCCGCTTAATTAAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGG                                                                    <  SRR19973061.631163/150‑1 (MQ=60)
                                                                                     TTCCGATTAATTCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGA                                                                <  SRR19973061.264505/150‑1 (MQ=60)
                                                                                      TCCGCTTAATTCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                               <  SRR19973061.2449810/150‑1 (MQ=60)
                                                                                      TCCGCTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                               <  SRR19973061.2449815/150‑1 (MQ=60)
                                                                                      TCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                               >  SRR19973061.3134637/1‑150 (MQ=60)
                                                                                      TCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                               >  SRR19973061.3180327/1‑150 (MQ=60)
                                                                                         tataaattaaaactctaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                            <  SRR19973061.369396/132‑1 (MQ=60)
                                                                                         GCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                            >  SRR19973061.266817/1‑150 (MQ=60)
                                                                                            TAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTG                                                         >  SRR19973061.3298978/1‑150 (MQ=60)
                                                                                              attaaaacgctaaAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                       <  SRR19973061.1929285/137‑1 (MQ=60)
                                                                                               TGCAAACGCGACAATACCAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATT                                                      <  SRR19973061.3075927/150‑1 (MQ=60)
                                                                                                  AAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTA                                                   >  SRR19973061.574107/1‑150 (MQ=60)
                                                                                                       CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATA                                              >  SRR19973061.1396671/1‑150 (MQ=60)
                                                                                                       CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATA                                              <  SRR19973061.1042911/150‑1 (MQ=60)
                                                                                                         ACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAA                                            >  SRR19973061.2971178/1‑150 (MQ=60)
                                                                                                               ACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCC                                      <  SRR19973061.1887864/150‑1 (MQ=60)
                                                                                                               ACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCC                                      <  SRR19973061.2126652/150‑1 (MQ=60)
                                                                                                               ACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCC                                      <  SRR19973061.2553481/150‑1 (MQ=60)
                                                                                                                 AAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                    >  SRR19973061.2563786/1‑150 (MQ=60)
                                                                                                                 AAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                    >  SRR19973061.2563775/1‑150 (MQ=60)
                                                                                                                  AACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                   <  SRR19973061.1399485/150‑1 (MQ=60)
                                                                                                                  AACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                   <  SRR19973061.1399431/150‑1 (MQ=60)
                                                                                                                  AAAAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                   <  SRR19973061.1398867/150‑1 (MQ=60)
                                                                                                                   ACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGA                                  <  SRR19973061.2440733/150‑1 (MQ=60)
                                                                                                                     AAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                                >  SRR19973061.587906/1‑150 (MQ=60)
                                                                                                                     AAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                                >  SRR19973061.587231/1‑150 (MQ=60)
                                                                                                                       AGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                              <  SRR19973061.1079344/150‑1 (MQ=60)
                                                                                                                        GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGA                             <  SRR19973061.2310047/150‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                           >  SRR19973061.10678/1‑150 (MQ=60)
                                                                                                                            CCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGG                         <  SRR19973061.2205675/150‑1 (MQ=60)
                                                                                                                                 TCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAA                    >  SRR19973061.3027065/1‑150 (MQ=60)
                                                                                                                                   CATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAT                  >  SRR19973061.795792/1‑150 (MQ=60)
                                                                                                                                    ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                 <  SRR19973061.430754/150‑1 (MQ=60)
                                                                                                                                    ATACGCACTCTGCACGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                 <  SRR19973061.1893650/150‑1 (MQ=60)
                                                                                                                                      ACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAT               <  SRR19973061.48612/150‑1 (MQ=60)
                                                                                                                                      ACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAT               <  SRR19973061.3152374/150‑1 (MQ=60)
                                                                                                                                      ACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAT               <  SRR19973061.298424/150‑1 (MQ=60)
                                                                                                                                       CTCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCAATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG              <  SRR19973061.2084/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG              <  SRR19973061.2241504/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG              <  SRR19973061.1780783/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG              <  SRR19973061.328048/150‑1 (MQ=60)
                                                                                                                                             CTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGG        <  SRR19973061.780464/150‑1 (MQ=60)
                                                                                                                                             CTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGG        <  SRR19973061.3545601/150‑1 (MQ=60)
                                                                                                                                               GCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG      >  SRR19973061.1683919/1‑150 (MQ=60)
                                                                                                                                                  CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT   <  SRR19973061.488943/150‑1 (MQ=60)
                                                                                                                                                   GTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTC  <  SRR19973061.25691/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTC  >  CP009273/2404623‑2404919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑