Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA98.6% 669.0 / 0.5 208A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (120/85);  total (122/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404629‑2404916
                                                                                                                                               |                                                                                                                                                
tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaa                                                                                                                                              >  2:2075155/1‑148 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                       >  2:1955506/1‑148 (MQ=255)
         ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                     >  1:1239426/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    >  1:2056343/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    >  2:815587/1‑148 (MQ=255)
          aaaactcggcgccAATCAGGAAAACTTTCGTATAAGAAACGCCTTAAAATACTTCTGGGTCTGTCCGCGTTCCGATTAATCAAAACGCTACAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    <  2:1585267/141‑1 (MQ=255)
             aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGc                                                                                                                                 >  1:2618076/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCac                                                                                                                               >  2:3485827/1‑147 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCaa                                                                                                                               >  1:2604278/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGAAGCAAACAAGACGATGGCaa                                                                                                                               >  2:2839499/1‑148 (MQ=255)
                aGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                              >  2:2528352/1‑148 (MQ=255)
                aGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                              >  1:1514114/1‑148 (MQ=255)
                 ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                             >  1:1289061/1‑148 (MQ=255)
                     gcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCa                                                                                                                         >  2:341549/1‑148 (MQ=255)
                           aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                                                   >  1:3493390/1‑148 (MQ=255)
                           aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                                                   >  1:3493379/1‑148 (MQ=255)
                             gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                 >  1:153315/1‑148 (MQ=255)
                             gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                 >  1:1928024/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAACAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:2539774/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:3314292/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:3234500/1‑148 (MQ=255)
                                   tttAGTATCACAAAAGCCGTAAAATACTTCTGGGACTGTCCGCGTTCCGATAAATCCAAACGCGACAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                                                                                                           <  2:3151876/147‑1 (MQ=255)
                                     tAGTATCATAAACGCCTTAAAATAGTTGGGGTACTGTCAGCGTTCCGCTTAATCAAAACGCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                         <  2:160959/148‑1 (MQ=255)
                                          tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                    >  2:3008327/1‑148 (MQ=255)
                                             gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAACGAACCCAAATCAAtt                                                                                                 >  2:2008019/1‑148 (MQ=255)
                                              aaaaGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGAAAACGCTAAATTAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                <  2:1755747/148‑1 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:2622113/1‑148 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:600762/1‑148 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:2974908/1‑148 (MQ=255)
                                               aacgCCGTAAATTAGTTCTGGTACTTTCAGGTTTCCGATTAATCAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                               <  2:723543/145‑1 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCATCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATACAAACGCACTCTGCCCGTAGCAAACAAGACGATGGAAACGGCCAGTAATGAAAGAACCCAAATCAAATCAc                                                                                              >  1:507521/1‑148 (MQ=255)
                                                 aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                             >  1:2536217/1‑148 (MQ=255)
                                                 aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                             >  2:1244749/1‑148 (MQ=255)
                                                  gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                            >  2:698221/1‑148 (MQ=255)
                                                  gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                            >  1:414913/1‑148 (MQ=255)
                                                     ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                         >  2:1939215/1‑148 (MQ=255)
                                                       aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                                                       >  2:2271/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                     >  1:1774775/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTAAGGGTTCCGCTTAATGAAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                     <  2:2618076/148‑1 (MQ=255)
                                                          aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAAc                                                                                    >  2:1003412/1‑148 (MQ=255)
                                                                 tGGGAGTGTCCGGTTTCCGTTTAATGCAACCGCCAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCt                                                                             <  1:2909904/148‑1 (MQ=255)
                                                                          cAGGGTTCCGATTAATCAAAACGCCACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    <  2:2339669/148‑1 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    >  2:1015530/1‑148 (MQ=255)
                                                                          aaGCGTTCCGCTTATTGAAAACCCTAAAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAACGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    <  2:2640528/147‑1 (MQ=255)
                                                                               ttCCGCTTAATCAAACCGCCAAAAAAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg                                                               <  2:669540/148‑1 (MQ=255)
                                                                                tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              >  2:1725738/1‑148 (MQ=255)
                                                                                tCCCCTTAATCAAAACCCGAAAAAAACAAACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              <  2:2470853/148‑1 (MQ=255)
                                                                                 ccGATTAATCAAAACGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                                             <  2:1466650/148‑1 (MQ=255)
                                                                                    cTTACTCCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                          <  2:692127/148‑1 (MQ=255)
                                                                                     ttAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                                                         >  2:1575757/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:824359/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:834411/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:924193/1‑148 (MQ=255)
                                                                                         tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                     >  1:683953/1‑148 (MQ=255)
                                                                                         tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                     >  2:1395293/1‑148 (MQ=255)
                                                                                          gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                    >  2:2455294/1‑148 (MQ=255)
                                                                                           cAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                   >  1:2693848/1‑148 (MQ=255)
                                                                                                  taaaaTAACAAACACAGCGACCGCATCCATACGCACTCGGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCAt                                            <  2:3013649/145‑1 (MQ=255)
                                                                                                      aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                        <  2:1289236/148‑1 (MQ=255)
                                                                                                       tAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       >  2:2170238/1‑148 (MQ=255)
                                                                                                       tAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       >  2:2040360/1‑148 (MQ=255)
                                                                                                       aaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       <  1:2344211/144‑1 (MQ=255)
                                                                                                         acaaacaaAGCGACCGCATCCATACGAAATCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAAACAAATAAATTCACCTTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                     >  1:2278705/1‑148 (MQ=255)
                                                                                                         aaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                     <  1:295924/146‑1 (MQ=255)
                                                                                                           aaacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   <  1:2990855/148‑1 (MQ=255)
                                                                                                            aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                  >  2:1371084/1‑148 (MQ=255)
                                                                                                            aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                  >  1:729480/1‑148 (MQ=255)
                                                                                                               aacgCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcga                               <  2:1334707/145‑1 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             <  2:405773/148‑1 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  1:1372249/1‑148 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATACACCGTTCACAACTTATCATTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  1:593627/1‑148 (MQ=255)
                                                                                                                   cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                           >  1:2802713/1‑148 (MQ=255)
                                                                                                                    gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                          <  1:977572/148‑1 (MQ=255)
                                                                                                                     aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCTACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                         <  1:1384479/148‑1 (MQ=255)
                                                                                                                     aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                         >  1:107526/1‑148 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCATTGAGGGGGATGACTTGATTCTGCCATACAAAAGCCCCGACGAGACgg                       >  1:3238305/1‑148 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                       <  1:1003389/148‑1 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                       <  1:1004222/148‑1 (MQ=255)
                                                                                                                        gCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                      <  1:2176758/148‑1 (MQ=255)
                                                                                                                         cATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                     >  1:1408014/1‑148 (MQ=255)
                                                                                                                          aTCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGc                    >  1:2372292/1‑148 (MQ=255)
                                                                                                                            ccATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                  >  2:2676797/1‑148 (MQ=255)
                                                                                                                              agaCGAACTATGCCCGTATCAAACAAGACTATGGCAACGGCCATTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGTGATGACTTTATTCTGCCATAAAAAAGCCCCGACGAGACGTGGCTaaa                <  2:1285468/146‑1 (MQ=255)
                                                                                                                              aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                <  1:3091913/148‑1 (MQ=255)
                                                                                                                              aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                <  1:533208/148‑1 (MQ=255)
                                                                                                                                aCGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc              >  2:91691/1‑148 (MQ=255)
                                                                                                                                 cGCACTCTGCCCGTATCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa             <  2:1900952/148‑1 (MQ=255)
                                                                                                                                   cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           <  2:3493385/148‑1 (MQ=255)
                                                                                                                                   cACTCTGCCCGTAGAAAACAAGACGAGGGCAACGGCAAGTAATTAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           <  1:3271029/148‑1 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          <  2:1239426/148‑1 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          >  2:2857586/1‑148 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          <  1:1682405/148‑1 (MQ=255)
                                                                                                                                     ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt         <  1:2848974/148‑1 (MQ=255)
                                                                                                                                     ctctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATAAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt         <  2:2218447/148‑1 (MQ=255)
                                                                                                                                      tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc        >  1:2148556/1‑148 (MQ=255)
                                                                                                                                       ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa       <  2:460285/148‑1 (MQ=255)
                                                                                                                                       ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa       <  1:207649/148‑1 (MQ=255)
                                                                                                                                        tGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAg      <  2:1483859/148‑1 (MQ=255)
                                                                                                                                            cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  2:1514116/148‑1 (MQ=255)
                                                                                                                                            cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  1:2253620/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404629‑2404916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404624‑2404918
                                                                                                                                                        |                                                                                                                                                  
AACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAG                                                                                                                                                   >  SRR19973065.2277033/1‑150 (MQ=60)
AACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAG                                                                                                                                                   >  SRR19973065.2486413/1‑150 (MQ=60)
      cgcaaaaactacacagggcggcaatctggaaaaggttagtaacacaaatgcggtaaaatacttgtgggactgaccgcgttccgataaacccaaccgctaaaaaAACAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACA                                                                                                                                             <  SRR19973065.589468/47‑1 (MQ=60)
            TAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGA                                                                                                                                       >  SRR19973065.1247565/1‑150 (MQ=60)
             AATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAT                                                                                                                                      >  SRR19973065.2069472/1‑150 (MQ=60)
                AAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                   >  SRR19973065.2634291/1‑150 (MQ=60)
                 AACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCA                                                                                                                                  >  SRR19973065.2309708/1‑150 (MQ=60)
                  ACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAA                                                                                                                                 >  SRR19973065.2620446/1‑150 (MQ=60)
                   ccaggcggaaatcagcacaactttagtaaaacaaaagccttAAAATACTTCTGGGACTGTCAGGGTTCCGATAAATCCAAACGCTAAAAAAACAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973065.3213816/109‑1 (MQ=60)
                   ccaggcgcaaatctgtaaaagttttttaaaataaaagcattaaattagttgtgggagtgtccgggttccttttaatcaaaaccccaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973065.443458/57‑1 (MQ=60)
                   CAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                >  SRR19973065.1523999/1‑150 (MQ=60)
                   CAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                >  SRR19973065.1524001/1‑150 (MQ=60)
                   CAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                >  SRR19973065.588067/1‑150 (MQ=60)
                    agggggcaaaccctcaaaattttagtataaaaacacccgtaaatttgtggtgggtgtgtctgggttccgcttatccaaaaccaaacaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               <  SRR19973065.2588887/58‑1 (MQ=60)
                    AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               >  SRR19973065.1297560/1‑150 (MQ=60)
                    AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               >  SRR19973065.1297736/1‑150 (MQ=60)
                       cccggcccaatcacgaaaaagtttcttaccacaaccgcccaaaaatatttcgggaagggacccgttcccgatttagcaaaaccaaaaaaacaaaaaccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973065.443430/50‑1 (MQ=60)
                       cacggcgaaaagcataacagttttagtacaaaaaaccccttaaaatattggtggtactctcgggttccctttaaaacaaaacccaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973065.443451/50‑1 (MQ=60)
                         gcaattctgtaatatttttgtataacaaatgcgttaaattttttgtgggactttctggttgcctattaatcaaacctccaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAG                                                                                                                          <  SRR19973065.291212/63‑1 (MQ=60)
                         GGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAG                                                                                                                          >  SRR19973065.2995477/1‑150 (MQ=60)
                            ANTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                       >  SRR19973065.2656829/1‑150 (MQ=60)
                            AATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                       >  SRR19973065.2290265/1‑150 (MQ=60)
                              TCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATG                                                                                                                     >  SRR19973065.3514616/1‑150 (MQ=60)
                              TCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATG                                                                                                                     >  SRR19973065.3514627/1‑150 (MQ=60)
                              TCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATG                                                                                                                     >  SRR19973065.3514622/1‑150 (MQ=60)
                                AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973065.565105/1‑150 (MQ=60)
                                AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973065.154599/1‑150 (MQ=60)
                                AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973065.1940657/1‑150 (MQ=60)
                                 atattcacaatgttattaaaaaaaacccctaaaaaaattggtgtgcggggcgggttactgtaaaaaaaaacccaaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAT                                                                                                                      <  SRR19973065.2096685/60‑1 (MQ=60)
                                  acatcaaacctttttaaaaaaaaaccccttaaaatatttgggggactgtctggtttccgattaatcaaaacccaaaaaaaaaaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATG                                                                                                                     <  SRR19973065.1079942/61‑1 (MQ=60)
                                  aaaaaatttagtataataaatgccttaatatatttttgggagtgtcagcgtaccttttaatccacccgccaaAATAACAAAAAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                 <  SRR19973065.2641987/78‑1 (MQ=60)
                                   ACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGA                                                                                                                >  SRR19973065.2384110/1‑150 (MQ=60)
                                           AGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                        >  SRR19973065.3399628/1‑150 (MQ=60)
                                            tttataaaaataaaaccttaaaattttttgggtgtgtttctggtttcttaaaaataaaaaccaaaaaaaaacaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCA                                                                                                           <  SRR19973065.1221374/71‑1 (MQ=60)
                                             taacataaatgccttaaattacttgtgggtgtgtccgcttccctattaatcaaaacgctaaAATAACAAACACC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCA                                                                                                      <  SRR19973065.1760896/89‑1 (MQ=60)
                                                aaaaaatgccatataattgttgtggtagtttcaggtttcctataattaaaacctctaaAATAACAAAAAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGAAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATT                                                                                                   <  SRR19973065.2519769/92‑1 (MQ=60)
                                                   AAAAGCCGGAAAATACTTCTGGGACTGTCATCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAA‑‑‑‑GCGACCGCATACAAACGCACTCTGCCCGTAGCAAACAAGACGATGGAAACGGCCAGTAATGAAAGAACCCAAATCAAATCAC                                                                                                >  SRR19973065.510900/1‑150 (MQ=60)
                                                    aatgctgtaaatttgttctggtactttcgggtttcggtttattcaaaactctaaattaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACC                                                                                               <  SRR19973065.1408025/90‑1 (MQ=60)
                                                    AAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACC                                                                                               >  SRR19973065.2552125/1‑150 (MQ=60)
                                                    AAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACC                                                                                               >  SRR19973065.2252353/1‑150 (MQ=60)
                                                     ataaactccttaaattatttgtgttactgtttggtttgctataaatcaaaccccaaaaaaaaaaaccaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTC                                                                                                  <  SRR19973065.60706/80‑1 (MQ=60)
                                                     AAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                              >  SRR19973065.417821/1‑150 (MQ=60)
                                                      accttaaaaatttttgggtgagtttcgggtttccttttaaacaaaacgaaaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                             <  SRR19973065.1809065/92‑1 (MQ=60)
                                                       gctttaaattatttttgggagtgtcggctttcctattattaaacacgctaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTT                                                                                            <  SRR19973065.2153005/93‑1 (MQ=60)
                                                       cctttaaattagttgtgggtgttcctgttttcctataaatcaaaccccaaaaaaaaaaaaaacA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTT                                                                                            <  SRR19973065.1123685/87‑1 (MQ=60)
                                                          agcttaaaattatttttggtactttccgttttcgtattaacaaaaaccaaaaaaaacaaaaccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGT                                                                                             <  SRR19973065.18327/85‑1 (MQ=60)
                                                           gcctaaaaatatttggttgactgactggtttcttattataaaaacctacaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTT                                                                                            <  SRR19973065.1006160/86‑1 (MQ=60)
                                                            AAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAA                                                                                       >  SRR19973065.1786384/1‑150 (MQ=60)
                                                                aaattcgtttgggtagtgtccgcttaccgataaagcaaaccgcaaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAA                                                                                       <  SRR19973065.1293759/91‑1 (MQ=60)
                                                                 ACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                  >  SRR19973065.983259/1‑150 (MQ=60)
                                                                 ACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                  >  SRR19973065.1296220/1‑150 (MQ=60)
                                                                    tgtgggtctgtctggtttcctattaatcaaaactcaaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCT                                                                               <  SRR19973065.2418191/106‑1 (MQ=60)
                                                                     attgggggttcgtgctggggcccgtttaatcaaaccgaaaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAACGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                  <  SRR19973065.3296145/96‑1 (MQ=60)
                                                                      tgggagtgtccggtttccgtttaatgcaaccgccaaaaaAACAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                             <  SRR19973065.2927621/111‑1 (MQ=60)
                                                                        tcgggtattgtgtgtttcccttttactcaaaacgccaaaaaaaaaaaaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCT                                                                               <  SRR19973065.2420596/99‑1 (MQ=60)
                                                                        ggactgtcagcttaccgattaatcaaaaccctaaAATAAAAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTT                                                                           <  SRR19973065.1171561/116‑1 (MQ=60)
                                                                                ggtctgggtcccttataaccaaaccccaaaaaaaaaacacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATT                                                                       <  SRR19973065.1545979/107‑1 (MQ=60)
                                                                                AGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGG                                                                   >  SRR19973065.2187055/1‑150 (MQ=60)
                                                                                AGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGG                                                                   >  SRR19973065.886020/1‑150 (MQ=60)
                                                                                   GTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGG                                                                >  SRR19973065.3074179/1‑150 (MQ=60)
                                                                                    tgggtccctattaatccaaacccaaaaaaacaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGG                                                                   <  SRR19973065.1968284/111‑1 (MQ=60)
                                                                                       gtccctattacacaaaacgccaaaaaaacaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGG                                                                <  SRR19973065.176206/114‑1 (MQ=60)
                                                                                        tcccgtttaatccaaaccctacaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGA                                                               <  SRR19973065.1142739/115‑1 (MQ=60)
                                                                                         CTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          >  SRR19973065.839848/1‑150 (MQ=60)
                                                                                         CTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          >  SRR19973065.839855/1‑150 (MQ=60)
                                                                                         CTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          >  SRR19973065.829768/1‑150 (MQ=60)
                                                                                         CTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          >  SRR19973065.930255/1‑150 (MQ=60)
                                                                                         CTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          >  SRR19973065.429084/1‑150 (MQ=60)
                                                                                            cgttaaaaaaaaccaaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973065.1208934/119‑1 (MQ=60)
                                                                                            AATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGA                                                       >  SRR19973065.688502/1‑150 (MQ=60)
                                                                                            AATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGA                                                       >  SRR19973065.688501/1‑150 (MQ=60)
                                                                                             cttaatcaaaccgcaacaaaaaaaacaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          <  SRR19973065.2886491/120‑1 (MQ=60)
                                                                                              ccaaacccacaaaaaaaaAAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATT                                                     <  SRR19973065.1865737/132‑1 (MQ=60)
                                                                                              TGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATT                                                     >  SRR19973065.2710575/1‑150 (MQ=60)
                                                                                               aaaccaaaccccaaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTG                                                        <  SRR19973065.3311283/122‑1 (MQ=60)
                                                                                                 AAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTG                                                  >  SRR19973065.2928678/1‑150 (MQ=60)
                                                                                                  tcaaacccctaaaaaaacaaaaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATT                                                     <  SRR19973065.1072222/125‑1 (MQ=60)
                                                                                                  cccaaccccaacaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATT                                                     <  SRR19973065.1071611/125‑1 (MQ=60)
                                                                                                     aaccccaaaaaaaaaaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTG                                                  <  SRR19973065.373983/128‑1 (MQ=60)
                                                                                                            TAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       >  SRR19973065.519104/1‑150 (MQ=60)
                                                                                                            TAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       >  SRR19973065.1617603/1‑150 (MQ=60)
                                                                                                            TAACAAACAAA‑‑‑‑GCGACCGCATCCATACGAAATCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAAACAAATAAATTCACCTTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       >  SRR19973065.2293078/1‑150 (MQ=60)
                                                                                                               aaaaaaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAA                                        <  SRR19973065.1207217/138‑1 (MQ=60)
                                                                                                               CAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCC                                    >  SRR19973065.1732436/1‑150 (MQ=60)
                                                                                                               CAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCC                                    >  SRR19973065.734364/1‑150 (MQ=60)
                                                                                                                aaacaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973065.1225369/139‑1 (MQ=60)
                                                                                                                aaaaacacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCATCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973065.232546/139‑1 (MQ=60)
                                                                                                                aaaaaacccccGCGACCGCATCCATACGCACTCTGCACGTAGCAAACAAGACGATGTCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACTACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973065.3286795/139‑1 (MQ=60)
                                                                                                                aaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973065.2358834/139‑1 (MQ=60)
                                                                                                                AAACAAC‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                   <  SRR19973065.3009164/150‑1 (MQ=60)
                                                                                                                AAACAAAAACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973065.3286810/150‑1 (MQ=60)
                                                                                                                  aaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCC                                     <  SRR19973065.297825/141‑1 (MQ=60)
                                                                                                                    AAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                               >  SRR19973065.1037599/1‑150 (MQ=60)
                                                                                                                    AAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                               >  SRR19973065.1381305/1‑150 (MQ=60)
                                                                                                                    AAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                               >  SRR19973065.1381325/1‑150 (MQ=60)
                                                                                                                    AAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATACACCGTTCACAACTTATCATTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACG                               >  SRR19973065.597293/1‑150 (MQ=60)
                                                                                                                      A‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                             >  SRR19973065.2819995/1‑150 (MQ=60)
                                                                                                                      A‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                             >  SRR19973065.2819741/1‑150 (MQ=60)
                                                                                                                      A‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                             >  SRR19973065.2820067/1‑150 (MQ=60)
                                                                                                                       caccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGAC                                <  SRR19973065.1441050/146‑1 (MQ=60)
                                                                                                                            CGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAC                           >  SRR19973065.108506/1‑150 (MQ=60)
                                                                                                                             GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973065.984062/150‑1 (MQ=60)
                                                                                                                              ACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCTACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGG                         <  SRR19973065.1393586/150‑1 (MQ=60)
                                                                                                                              ACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCATTGAGGGGGATGACTTGATTCTGCCATACAAAAGCCCCGACGAGACGG                         >  SRR19973065.3257806/1‑150 (MQ=60)
                                                                                                                              ACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGG                         <  SRR19973065.1393822/150‑1 (MQ=60)
                                                                                                                                CGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGG                       <  SRR19973065.1010053/150‑1 (MQ=60)
                                                                                                                                CGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGG                       >  SRR19973065.1417229/1‑150 (MQ=60)
                                                                                                                                CGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGG                       <  SRR19973065.1010076/150‑1 (MQ=60)
                                                                                                                                CGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGG                       <  SRR19973065.1010890/150‑1 (MQ=60)
                                                                                                                                CGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGG                       <  SRR19973065.1951902/150‑1 (MQ=60)
                                                                                                                                 GCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGC                      >  SRR19973065.2387100/1‑150 (MQ=60)
                                                                                                                                 GCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGC                      <  SRR19973065.2190718/150‑1 (MQ=60)
                                                                                                                                  CATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCT                     <  SRR19973065.2626582/150‑1 (MQ=60)
                                                                                                                                       ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                <  SRR19973065.820957/150‑1 (MQ=60)
                                                                                                                                       ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                <  SRR19973065.536689/150‑1 (MQ=60)
                                                                                                                                       ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                <  SRR19973065.3110793/150‑1 (MQ=60)
                                                                                                                                       ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATC                <  SRR19973065.1944767/150‑1 (MQ=60)
                                                                                                                                       ATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTAACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGACATAAAAAAGCCCCGACGAGACGGGGCTAAATA                >  SRR19973065.2830760/1‑150 (MQ=60)
                                                                                                                                            CACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAT           <  SRR19973065.702867/150‑1 (MQ=60)
                                                                                                                                            CACTCTGCCCGTAGAAAACAAGACGAGGGCAACGGCAAGTAATTAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAT           <  SRR19973065.3290877/150‑1 (MQ=60)
                                                                                                                                             ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          >  SRR19973065.385224/1‑150 (MQ=60)
                                                                                                                                             ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          >  SRR19973065.2162370/1‑150 (MQ=60)
                                                                                                                                             ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          <  SRR19973065.1693659/150‑1 (MQ=60)
                                                                                                                                              CTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCA         <  SRR19973065.2866499/150‑1 (MQ=60)
                                                                                                                                              CTCGGCCCGTAGCAAACAAGCCGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCA         <  SRR19973065.1818991/150‑1 (MQ=60)
                                                                                                                                                CTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGG       <  SRR19973065.209219/150‑1 (MQ=60)
                                                                                                                                                CTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGG       <  SRR19973065.209224/150‑1 (MQ=60)
                                                                                                                                                   CCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAAAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT    <  SRR19973065.2567997/150‑1 (MQ=60)
                                                                                                                                                     CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  SRR19973065.3018736/1‑150 (MQ=60)
                                                                                                                                                     CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  <  SRR19973065.2267898/150‑1 (MQ=60)
                                                                                                                                                     CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  SRR19973065.3017694/1‑150 (MQ=60)
                                                                                                                                                        |                                                                                                                                                  
AACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAA‑‑‑‑GCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404624‑2404918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑