Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA98.7% 732.3 / ‑0.9 226A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (138/85);  total (141/85)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

CATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404627‑2404916
                                                                                                                                                 |                                                                                                                                                
cATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGc                                                                                                                                                >  1:2612985/1‑148 (MQ=255)
     ccAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACa                                                                                                                                           >  2:3263359/1‑148 (MQ=255)
      cAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                          >  2:2277526/1‑148 (MQ=255)
        ataataAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGa                                                                                                                                        >  2:2482569/1‑148 (MQ=255)
          aataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACg                                                                                                                                      >  1:1107226/1‑148 (MQ=255)
           ataaACCAGGCGCAAATCACGACAACTTTAGGATCACAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTACCGATTAATCAAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                     <  2:1969684/148‑1 (MQ=255)
           ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                     >  1:1762018/1‑148 (MQ=255)
           ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                     >  1:1761885/1‑148 (MQ=255)
             aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATg                                                                                                                                   >  2:3066997/1‑148 (MQ=255)
              aaaacggcggaAATCTGCACAACTTTAGTAAAATAAAAGCCTAAAATTACTTCTGGGAGTGTAAGGTTTCGGATAAATCAAAACGCTAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATgg                                                                                                                                  <  2:368610/143‑1 (MQ=255)
               aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGc                                                                                                                                 >  1:149943/1‑148 (MQ=255)
                   ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                             >  1:3657491/1‑148 (MQ=255)
                   ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                             >  2:1382776/1‑148 (MQ=255)
                   ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                             >  2:1727044/1‑148 (MQ=255)
                        cAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAg                                                                                                                        >  1:2748767/1‑148 (MQ=255)
                          agcAGCACAACGTTAGGATCATAAAAGCCGCAAAATACTTCTGGGCCTGTCAGCTTTCCGATTAAGCCAACCGCTACAATAACAACCAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTa                                                                                                                      <  2:1219277/146‑1 (MQ=255)
                           tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                     >  1:902827/1‑148 (MQ=255)
                            ccggACTACGTTCGGACAATAAAAGCCGTAAAATACTTGTGGGACTGACCGGGTACCGCTTACTCAAAACCCCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                    <  2:1770751/146‑1 (MQ=255)
                            aggtaCAACTTTCGGAACATAAACGCCTTAAAATACTTCTGGGACTTTCAGCGTTCCGCTTAATCAAACCGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                    <  2:2128985/144‑1 (MQ=255)
                               gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                 >  1:1234980/1‑148 (MQ=255)
                                 cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:2461263/1‑148 (MQ=255)
                                   aCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                                                                             >  1:1017442/1‑148 (MQ=255)
                                   aCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                                                                             >  1:1365777/1‑148 (MQ=255)
                                       tAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                         >  2:1329104/1‑148 (MQ=255)
                                       tAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                         >  2:397172/1‑148 (MQ=255)
                                          gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                                                      >  1:2403828/1‑148 (MQ=255)
                                          gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                                                      >  1:2403885/1‑148 (MQ=255)
                                            taaaaaaaGCCTTAAAATACTTGTGTGACTGTCCGGGTTCCGATTAATCCAAAGGCTAAAATAACAAAAACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                    <  2:1121575/144‑1 (MQ=255)
                                            tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                    >  1:582835/1‑148 (MQ=255)
                                              ataaaaGCCGTAAAATAGTTGTGGGACTGTCCGGGTTCCGATTAATCCAAACGCTAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                                                  <  2:819895/146‑1 (MQ=255)
                                               taaaaGCCGTAAAATATTTAGGTGACTTTCAGCTTTCCGATTATTGAAAACGCGAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                 <  2:3793611/147‑1 (MQ=255)
                                                aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:1669718/1‑148 (MQ=255)
                                                  aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                              >  2:2872968/1‑148 (MQ=255)
                                                   aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                             >  2:1691728/1‑148 (MQ=255)
                                                    gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                            >  1:1082433/1‑148 (MQ=255)
                                                          aaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                                                      >  2:2985584/1‑148 (MQ=255)
                                                          aaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                                                      >  2:2296363/1‑148 (MQ=255)
                                                          aaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                                                      >  2:3502692/1‑148 (MQ=255)
                                                          aaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                                                      >  2:3010669/1‑148 (MQ=255)
                                                           aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                     >  1:1718825/1‑148 (MQ=255)
                                                              aCTTCTGGTACGTACAGGGTTCCGATTAATCAAAACGCCACAACAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                  <  2:902827/148‑1 (MQ=255)
                                                              aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                  >  1:3743903/1‑148 (MQ=255)
                                                              aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                  >  2:502948/1‑148 (MQ=255)
                                                                     ggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                           >  1:545160/1‑148 (MQ=255)
                                                                     ggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                           >  1:2800585/1‑148 (MQ=255)
                                                                      gagtGTCTGCTTTCCTATTAATCAAAACGCCAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                                                                          <  1:3095832/145‑1 (MQ=255)
                                                                        gtGTAAGCTTTCCGTTTAATGAAAACGCTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                        <  2:3809603/147‑1 (MQ=255)
                                                                        cTGTCAGGTTACCGATAAATCAAAACCCCAAAAAAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                        <  1:2342486/148‑1 (MQ=255)
                                                                           tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                                                     >  1:1518012/1‑148 (MQ=255)
                                                                            cAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    <  2:2204185/148‑1 (MQ=255)
                                                                              gCGTTCCGCTAAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCAAACGCAAACTGCACCACCAACACAAGACGAAGGAAACGGCAACTAATCAAACAACCCAAATCAATTCACCGTACACAACTTAACCTTGGTAATTCAg                                                                  >  2:2048494/1‑148 (MQ=255)
                                                                                  tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              >  1:1317214/1‑148 (MQ=255)
                                                                                  tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              >  2:1341972/1‑148 (MQ=255)
                                                                                     gattAATCCAACCGCCAAAAAAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                           <  1:3125289/146‑1 (MQ=255)
                                                                                     gCTTACTCCAAACGCGACAACACCAACCCCCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAGCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                           <  1:2504424/148‑1 (MQ=255)
                                                                                      attAATCAAACCGCCAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                          <  1:2214482/147‑1 (MQ=255)
                                                                                        aaacccAAACGCCAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        <  1:3387228/143‑1 (MQ=255)
                                                                                           tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                     >  2:668240/1‑148 (MQ=255)
                                                                                            gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                    >  1:1984569/1‑148 (MQ=255)
                                                                                            gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                    >  1:382022/1‑148 (MQ=255)
                                                                                             cAACCGCGACAATAACAAACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                   <  2:1695345/148‑1 (MQ=255)
                                                                                                aCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                                                >  2:526047/1‑148 (MQ=255)
                                                                                                aCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                                                >  1:322099/1‑148 (MQ=255)
                                                                                                aCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                                                >  2:526034/1‑148 (MQ=255)
                                                                                                      cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                          >  1:1651234/1‑148 (MQ=255)
                                                                                                      cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCAGaa                                          >  2:3450015/1‑148 (MQ=255)
                                                                                                      cAATAACAAACAAAGCGACCGAATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATAGAGGGGGATGACTTGATTCTGCCATaa                                          >  1:3546607/1‑148 (MQ=255)
                                                                                                        aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                        >  1:969339/1‑148 (MQ=255)
                                                                                                        aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                        >  1:2880044/1‑148 (MQ=255)
                                                                                                         aaaaaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       <  1:3048750/144‑1 (MQ=255)
                                                                                                          aacaaacaAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaaa                                      >  1:1930116/1‑148 (MQ=255)
                                                                                                           acaaacaaCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                     <  2:3547380/148‑1 (MQ=255)
                                                                                                             aaacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   <  2:200978/148‑1 (MQ=255)
                                                                                                             aaacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   <  1:1282948/148‑1 (MQ=255)
                                                                                                             aaacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   <  1:197707/148‑1 (MQ=255)
                                                                                                             aaacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   >  2:1888796/1‑148 (MQ=255)
                                                                                                                caaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg                                >  2:103464/1‑148 (MQ=255)
                                                                                                                  aaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac                              >  1:1561500/1‑148 (MQ=255)
                                                                                                                  aaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac                              >  1:1561124/1‑148 (MQ=255)
                                                                                                                   aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  2:3224191/1‑148 (MQ=255)
                                                                                                                   aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  2:3151206/1‑148 (MQ=255)
                                                                                                                    gCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacga                            >  2:546573/1‑148 (MQ=255)
                                                                                                                    gCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacga                            >  1:1293114/1‑148 (MQ=255)
                                                                                                                    gCGACAGAATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATAAATTCAAAGTTAACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTATGCCATAAAAAAGCCCcgacga                            >  2:2450452/1‑148 (MQ=255)
                                                                                                                     cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                           <  2:1761886/148‑1 (MQ=255)
                                                                                                                      gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                          <  1:2998680/148‑1 (MQ=255)
                                                                                                                      gACCGCATCAATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                          <  1:1040957/148‑1 (MQ=255)
                                                                                                                             tCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCt                   >  1:1202420/1‑148 (MQ=255)
                                                                                                                                aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                >  1:1404857/1‑148 (MQ=255)
                                                                                                                                  aCGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc              <  1:2424824/148‑1 (MQ=255)
                                                                                                                                   cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa             <  1:2057739/148‑1 (MQ=255)
                                                                                                                                   cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa             <  1:2727658/148‑1 (MQ=255)
                                                                                                                                   cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa             <  1:479253/148‑1 (MQ=255)
                                                                                                                                    gCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAt            <  2:2822651/148‑1 (MQ=255)
                                                                                                                                     cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCCCCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           <  1:219214/148‑1 (MQ=255)
                                                                                                                                     cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           >  2:3811716/1‑148 (MQ=255)
                                                                                                                                      aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          <  1:126280/148‑1 (MQ=255)
                                                                                                                                           gCCCGTAGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg     <  1:3263359/148‑1 (MQ=255)
                                                                                                                                              cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  2:239298/148‑1 (MQ=255)
                                                                                                                                              cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  2:238492/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404627‑2404916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404623‑2404918
                                                                                                                                                     |                                                                                                                                                  
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                    >  SRR19973069.673273/1‑150 (MQ=60)
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTA                                                                                                                                                    >  SRR19973069.2604238/1‑150 (MQ=60)
  ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGC                                                                                                                                                  >  SRR19973069.2630451/1‑150 (MQ=60)
         caaataaacaacccggggcaaatctgcacttgtttcgtataacaaacgccttatttttttggtgggttgttgggggtgcctattaattaaaccgctaaaaaaaaaaaccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAG                                                                                                                                           <  SRR19973069.3641174/39‑1 (MQ=60)
            aaaaaaaccccggctcaaataacaacaatttttcaaaaaaaaaccccttaaaatcttggtggtagtgtccggtttcgtataaatcaaaacccaaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        <  SRR19973069.2641464/42‑1 (MQ=60)
            aaaaaaaactcggcggaatactctacatgtttagaaaaaaaaaagccttaaaataattctgtgacggtctgggttctcattaatcaacaccataaaaaaaaaaaccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        <  SRR19973069.2641477/42‑1 (MQ=60)
            ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        >  SRR19973069.1114989/1‑150 (MQ=60)
            ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        >  SRR19973069.240285/1‑150 (MQ=60)
            ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        >  SRR19973069.241095/1‑150 (MQ=60)
            ATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACG                                                                                                                                        >  SRR19973069.1114952/1‑150 (MQ=60)
             TAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGA                                                                                                                                       >  SRR19973069.1774309/1‑150 (MQ=60)
             TAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGA                                                                                                                                       >  SRR19973069.1774308/1‑150 (MQ=60)
             TAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGA                                                                                                                                       >  SRR19973069.1774442/1‑150 (MQ=60)
               ATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATG                                                                                                                                     >  SRR19973069.2116496/1‑150 (MQ=60)
                 AAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCCAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                   >  SRR19973069.129544/1‑150 (MQ=60)
                 AAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                   >  SRR19973069.151269/1‑150 (MQ=60)
                 AAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                   >  SRR19973069.129358/1‑150 (MQ=60)
                 AAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGC                                                                                                                                   >  SRR19973069.3435940/1‑150 (MQ=60)
                    ctcggcgtaaatctgcacattttttgtatcacaaacgcttcaaaattttggtgggagtgtctggtttccttttaatcaaaccgctaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973069.3392274/50‑1 (MQ=60)
                    caaggcgaaaatcataacaattttagtataacaaaagccttaaaatacttgtgggagtgtcggtgttccttttattcaaaaccaaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAC                                                                                                                                <  SRR19973069.3296647/50‑1 (MQ=60)
                     ctggagtaaataataaaaattttataaaaaaaaacgcataaaaatcgtgtgggtagtgtcctggtacctctaaaaccaaaccaaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               <  SRR19973069.2914212/51‑1 (MQ=60)
                     AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               >  SRR19973069.2655967/1‑150 (MQ=60)
                     AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               >  SRR19973069.3681440/1‑150 (MQ=60)
                     AAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACG                                                                                                                               >  SRR19973069.1777163/1‑150 (MQ=60)
                         CGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCA                                                                                                                           >  SRR19973069.2866394/1‑150 (MQ=60)
                          GGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAG                                                                                                                          >  SRR19973069.2767127/1‑150 (MQ=60)
                             attccttacaccttttgtaaaacaaacgcctcaaaatacttgggggactttccgcgttccgtttaatccaaccgctaaaaaaaaaacccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                       <  SRR19973069.593055/59‑1 (MQ=60)
                             aatcagcacatctttatgataataaacgccttaaaatacttgtggtattgtcgggtttggttttaagcaaaccgcaaaaaaaacaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                       <  SRR19973069.593538/59‑1 (MQ=60)
                             AATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAA                                                                                                                       >  SRR19973069.909025/1‑150 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973069.2592204/1‑150 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973069.1243391/1‑150 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973069.1318644/1‑150 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973069.1648240/1‑150 (MQ=60)
                                 AGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAA                                                                                                                   >  SRR19973069.1648234/1‑150 (MQ=60)
                                   taaattttttgtaataaaattgcattaatattttttggtttttgtatgttttgttattattcaaacgtaaaaaaaaaaaaaccacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                 <  SRR19973069.2988345/65‑1 (MQ=60)
                                   cacatttttctaataataaacgccttaaattagttggggtagtgtcagggttccttttaatcaaacccctaaAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                 <  SRR19973069.2094918/78‑1 (MQ=60)
                                   cacaccgtctgaaacaaaaaccccgaaaactccgggggggactgccggggtgccgctaaatccaaccccaaaaaacacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAG                                                                                                                 <  SRR19973069.2094889/65‑1 (MQ=60)
                                     aaatttttttaacataaaacccttaaaatagttgtgggactgtctgcgttcctattaatcaaaaccataaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAA                                                                                                               <  SRR19973069.2088908/67‑1 (MQ=60)
                                     aaattttattataaaaaacccttaaaatttttggggtctgggtccgggtccctattaatcaaacccacaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAA                                                                                                               <  SRR19973069.1467092/67‑1 (MQ=60)
                                     CAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAA                                                                                                               >  SRR19973069.1024466/1‑150 (MQ=60)
                                     CAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAA                                                                                                               >  SRR19973069.1375277/1‑150 (MQ=60)
                                            AGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAGACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                        >  SRR19973069.2420123/1‑150 (MQ=60)
                                            AGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                        >  SRR19973069.2420092/1‑150 (MQ=60)
                                            AGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                        >  SRR19973069.2420149/1‑150 (MQ=60)
                                            AGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAT                                                                                                        >  SRR19973069.2420154/1‑150 (MQ=60)
                                              GATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCA                                                                                                      >  SRR19973069.586829/1‑150 (MQ=60)
                                                 CAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATT                                                                                                   >  SRR19973069.2448606/1‑150 (MQ=60)
                                                      tcgcgttaaaatagttgtggtcatctctgtttggctcttatccaacacccaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                              <  SRR19973069.1368801/84‑1 (MQ=60)
                                                      atcgggaaaatttgttgtggtagttgcggggttccttttattaaaacccctaaaataaaaaccaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                              <  SRR19973069.550145/84‑1 (MQ=60)
                                                      AAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                              >  SRR19973069.1089993/1‑150 (MQ=60)
                                                      AAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCG                                                                                              >  SRR19973069.1089991/1‑150 (MQ=60)
                                                           GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAC                                                                                         >  SRR19973069.2284244/1‑150 (MQ=60)
                                                            GAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACA                                                                                        >  SRR19973069.3082744/1‑150 (MQ=60)
                                                             AAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAA                                                                                       >  SRR19973069.1730875/1‑150 (MQ=60)
                                                                ATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTT                                                                                    >  SRR19973069.3768297/1‑150 (MQ=60)
                                                                  atttctggtagtgactggtttccttttaatcaaaacccaaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAT                                                                                  <  SRR19973069.740969/106‑1 (MQ=60)
                                                                   CTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATC                                                                                 >  SRR19973069.2216945/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                             >  SRR19973069.1383876/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                             >  SRR19973069.2819181/1‑150 (MQ=60)
                                                                       TGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTG                                                                             >  SRR19973069.548985/1‑150 (MQ=60)
                                                                        GGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGT                                                                            >  SRR19973069.3790714/1‑150 (MQ=60)
                                                                          gagtgtctgctttcctattaatcaaaacgccaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTA                                                                          <  SRR19973069.3116233/114‑1 (MQ=60)
                                                                            gtgtcgggtgtcctttaactccaaccccaaaaaaaaaaacaaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAT                                                                        <  SRR19973069.444054/106‑1 (MQ=60)
                                                                            ctgtcaggttaccgataaatcaaaaccccaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAT                                                                        <  SRR19973069.2358413/106‑1 (MQ=60)
                                                                             TGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATT                                                                       >  SRR19973069.1528487/1‑150 (MQ=60)
                                                                              GTCAGCGTTCCGCTTAATGCACACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTG                                                                      >  SRR19973069.2177273/1‑150 (MQ=60)
                                                                              GTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAACGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAAGGCAAATGCCAGTAATGAAAGAACCCAAATCAATTAACCGTTCACAACTTATACTTGTTAATTG                                                                      >  SRR19973069.440246/1‑150 (MQ=60)
                                                                                ccgcgttccgattaatcaaaacccaaaAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAG                                                                    <  SRR19973069.3426069/123‑1 (MQ=60)
                                                                                CAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAG                                                                    >  SRR19973069.1299430/1‑150 (MQ=60)
                                                                                 tgggttcctttaactcaaacccccaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGG                                                                   <  SRR19973069.2140876/111‑1 (MQ=60)
                                                                                   gtttcgtattaaccaaaacgctaaaaaaacaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGG                                                                 <  SRR19973069.2805095/113‑1 (MQ=60)
                                                                                   CGTTCCCTTTAATCCAAACGCAACAAAAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGG                                                                 <  SRR19973069.2804994/150‑1 (MQ=60)
                                                                                    GTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGG                                                                >  SRR19973069.1326433/1‑150 (MQ=60)
                                                                                    GTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGG                                                                >  SRR19973069.1326253/1‑150 (MQ=60)
                                                                                      tcgttttatccaaacccagaaAATAAAAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAT                                                              <  SRR19973069.1551676/129‑1 (MQ=60)
                                                                                       cccataaaacaaacccaaaaaaaaaaacacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATG                                                             <  SRR19973069.668310/117‑1 (MQ=60)
                                                                                         tattaattcaaccgccaaaaaaaaaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973069.1229954/119‑1 (MQ=60)
                                                                                         tattaatcaaaacgcaaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973069.3145743/129‑1 (MQ=60)
                                                                                         gcttactccaaacgcgacaacaccaacccccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAGCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973069.2521136/119‑1 (MQ=60)
                                                                                         gattaatccaaccgccaaaaaAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973069.3145803/129‑1 (MQ=60)
                                                                                         cattaatcaaaacccaaaaaaacaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAC                                                           <  SRR19973069.1229926/126‑1 (MQ=60)
                                                                                          attaatcaaaccgccaaAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT                                                          <  SRR19973069.2229480/133‑1 (MQ=60)
                                                                                           TTAATGCAAACCCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTT                                                         <  SRR19973069.943401/150‑1 (MQ=60)
                                                                                            taatccaaccgccaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTG                                                        <  SRR19973069.3409348/132‑1 (MQ=60)
                                                                                            aaacccaaacgccaaaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTG                                                        <  SRR19973069.3409353/132‑1 (MQ=60)
                                                                                              ATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                      >  SRR19973069.384815/1‑150 (MQ=60)
                                                                                              ATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                      >  SRR19973069.1998164/1‑150 (MQ=60)
                                                                                              ATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAT                                                      >  SRR19973069.1998383/1‑150 (MQ=60)
                                                                                                gaaaaccccacaaaAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTC                                                    <  SRR19973069.1907991/136‑1 (MQ=60)
                                                                                                 aaaccgcaaaaaaaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT                                                   <  SRR19973069.3311732/127‑1 (MQ=60)
                                                                                                  AAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTG                                                  >  SRR19973069.324231/1‑150 (MQ=60)
                                                                                                       ctaaaaaaacaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATA                                             <  SRR19973069.509680/133‑1 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAA                                            >  SRR19973069.1662642/1‑150 (MQ=60)
                                                                                                        GACAATAACAAACAAAGCGACCGAATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATAGAGGGGGATGACTTGATTCTGCCATAA                                            >  SRR19973069.3569641/1‑150 (MQ=60)
                                                                                                          aaaaaaaAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                          <  SRR19973069.2810017/143‑1 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                          >  SRR19973069.975801/1‑150 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                          >  SRR19973069.1691769/1‑150 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                          >  SRR19973069.2898953/1‑150 (MQ=60)
                                                                                                          CAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAA                                          >  SRR19973069.1500106/1‑150 (MQ=60)
                                                                                                            ATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAA                                        >  SRR19973069.1943365/1‑150 (MQ=60)
                                                                                                             aaaaaaaaaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAG                                       <  SRR19973069.3068939/139‑1 (MQ=60)
                                                                                                                aaaccaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCC                                    <  SRR19973069.1062246/142‑1 (MQ=60)
                                                                                                                 AAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                   <  SRR19973069.1291535/150‑1 (MQ=60)
                                                                                                                 AAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCC                                   <  SRR19973069.199298/150‑1 (MQ=60)
                                                                                                                  accaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                  <  SRR19973069.3642495/144‑1 (MQ=60)
                                                                                                                  AACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCG                                  >  SRR19973069.3219637/1‑150 (MQ=60)
                                                                                                                    CAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGAC                                >  SRR19973069.1571779/1‑150 (MQ=60)
                                                                                                                    CAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGAC                                >  SRR19973069.1572152/1‑150 (MQ=60)
                                                                                                                    CAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGAC                                >  SRR19973069.1572155/1‑150 (MQ=60)
                                                                                                                      AAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA                              >  SRR19973069.1301795/1‑150 (MQ=60)
                                                                                                                      AAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA                              >  SRR19973069.2019687/1‑150 (MQ=60)
                                                                                                                       AGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                             >  SRR19973069.254496/1‑150 (MQ=60)
                                                                                                                       AGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAG                             >  SRR19973069.1536648/1‑150 (MQ=60)
                                                                                                                        GCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGA                            <  SRR19973069.285949/150‑1 (MQ=60)
                                                                                                                         CGACCGCATCCATACGCACTCTGCCCGTAGCAAACACGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAC                           <  SRR19973069.602058/150‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973069.1128958/150‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973069.3018578/150‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAATTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973069.3048505/150‑1 (MQ=60)
                                                                                                                          GACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAGTCACCGTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973069.1049091/150‑1 (MQ=60)
                                                                                                                          GACCGCATCAATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACG                          <  SRR19973069.1048193/150‑1 (MQ=60)
                                                                                                                               CATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAGTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCT                     >  SRR19973069.3132561/1‑150 (MQ=60)
                                                                                                                               CATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCT                     >  SRR19973069.1210689/1‑150 (MQ=60)
                                                                                                                                  CCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAA                  >  SRR19973069.290228/1‑150 (MQ=60)
                                                                                                                                  CCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAA                  >  SRR19973069.1414657/1‑150 (MQ=60)
                                                                                                                                   CATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAT                 >  SRR19973069.1964481/1‑150 (MQ=60)
                                                                                                                                      ACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAT              <  SRR19973069.2441190/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG             <  SRR19973069.482696/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG             <  SRR19973069.482729/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG             <  SRR19973069.2072012/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG             <  SRR19973069.2745926/150‑1 (MQ=60)
                                                                                                                                       CGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATG             <  SRR19973069.2748633/150‑1 (MQ=60)
                                                                                                                                         CACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCCCCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAT           <  SRR19973069.220920/150‑1 (MQ=60)
                                                                                                                                          ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCATTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          <  SRR19973069.127416/150‑1 (MQ=60)
                                                                                                                                          ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          <  SRR19973069.127466/150‑1 (MQ=60)
                                                                                                                                          ACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATC          >  SRR19973069.1468057/1‑150 (MQ=60)
                                                                                                                                           CTCTGCCCGTAGAAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCA         <  SRR19973069.1738075/150‑1 (MQ=60)
                                                                                                                                              TGCCCTTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGT      <  SRR19973069.3715429/150‑1 (MQ=60)
                                                                                                                                               GCCCGTAGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG     <  SRR19973069.3284737/150‑1 (MQ=60)
                                                                                                                                               GCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCACATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG     <  SRR19973069.3284695/150‑1 (MQ=60)
                                                                                                                                                CCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT    >  SRR19973069.1046960/1‑150 (MQ=60)
                                                                                                                                                  CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  <  SRR19973069.553920/150‑1 (MQ=60)
                                                                                                                                                  CGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  <  SRR19973069.1526840/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
AAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404623‑2404918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑