Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,459,976 G→T 93.3% intergenic (+114/‑48) argW → / → intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,459,9760GT93.3% 511.9 / 24.8 180intergenic (+114/‑48)argW/intStRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand):  ref base G (4/8);  new base T (78/90);  total (82/98)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 180 total aligned reads displayed.

AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCA  >  CP009273/2459831‑2460119
                                                                                                                                                 |                                                                                                                                               
aaTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAc                                                                                                                                               <  2:2017778/148‑1 (MQ=255)
aaTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAc                                                                                                                                               <  1:1731846/148‑1 (MQ=255)
         ttCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaaa                                                                                                                                      <  1:470927/148‑1 (MQ=255)
         ttCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaaa                                                                                                                                      <  1:3384988/148‑1 (MQ=255)
         ttCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaaa                                                                                                                                      <  2:303270/148‑1 (MQ=255)
          tCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAg                                                                                                                                     <  1:2957022/148‑1 (MQ=255)
             aTTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTgg                                                                                                                                  <  1:904412/148‑1 (MQ=255)
             aTTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTgg                                                                                                                                  >  1:93269/1‑148 (MQ=255)
             aTTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTgg                                                                                                                                  >  1:93271/1‑148 (MQ=255)
               tCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGAt                                                                                                                                <  1:3476107/148‑1 (MQ=255)
                 cTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaa                                                                                                                              <  2:612160/148‑1 (MQ=255)
                 cTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaa                                                                                                                              <  1:343779/148‑1 (MQ=255)
                  tGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaa                                                                                                                             <  2:2881908/148‑1 (MQ=255)
                  tGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaa                                                                                                                             <  2:2784312/148‑1 (MQ=255)
                   gCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaaa                                                                                                                            <  1:2076826/148‑1 (MQ=255)
                   gCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaaa                                                                                                                            <  1:280836/148‑1 (MQ=255)
                   gCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaaa                                                                                                                            <  1:2468467/148‑1 (MQ=255)
                      ggggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAaataa                                                                                                                         <  2:571952/148‑1 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                        >  1:1730923/1‑148 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                        >  1:2054928/1‑148 (MQ=255)
                        ggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAtt                                                                                                                       <  2:658858/148‑1 (MQ=255)
                         gACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAttt                                                                                                                      <  1:2481157/148‑1 (MQ=255)
                         gACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAttt                                                                                                                      <  1:3145520/148‑1 (MQ=255)
                             ccATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAcc                                                                                                                  >  1:2202494/1‑148 (MQ=255)
                             ccATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAcc                                                                                                                  >  1:3062263/1‑148 (MQ=255)
                              cATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAccc                                                                                                                 >  1:2636832/1‑148 (MQ=255)
                                tttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAc                                                                                                               <  1:2565240/148‑1 (MQ=255)
                                 ttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAcc                                                                                                              <  2:641859/148‑1 (MQ=255)
                                 ttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCAcc                                                                                                              >  2:126368/1‑148 (MQ=255)
                                     cAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAATTAATTTTACCCACCGGAt                                                                                                          <  2:3266721/148‑1 (MQ=255)
                                        ttCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAtttt                                                                                                       >  2:1801778/1‑148 (MQ=255)
                                          cGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTa                                                                                                     >  1:2432643/1‑148 (MQ=255)
                                             tCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTCGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTGTACCCACCGGATTTTTAccc                                                                                                  <  2:3306065/148‑1 (MQ=255)
                                                cATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCCTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATg                                                                                               <  1:2276728/148‑1 (MQ=255)
                                                 aTCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGc                                                                                              <  2:3502851/148‑1 (MQ=255)
                                                 aTCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGc                                                                                              >  2:448655/1‑148 (MQ=255)
                                                      tACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAcc                                                                                         >  2:2383014/1‑148 (MQ=255)
                                                      tACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAcc                                                                                         >  1:1095795/1‑148 (MQ=255)
                                                         cAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGtt                                                                                      >  1:3074197/1‑148 (MQ=255)
                                                            tCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAg                                                                                   >  1:1501291/1‑148 (MQ=255)
                                                             ccGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGc                                                                                  <  2:2373120/148‑1 (MQ=255)
                                                             ccGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGc                                                                                  <  2:2373040/148‑1 (MQ=255)
                                                                cAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATCAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGGTACGCAGa                                                                               >  2:2067723/1‑148 (MQ=255)
                                                                  aaaTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCCCCGTTAAGCAGAtt                                                                             >  2:2978401/1‑148 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTGAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                           >  1:1836265/1‑148 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                           >  2:3071831/1‑148 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                           <  2:2803547/148‑1 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                           <  2:1141507/148‑1 (MQ=255)
                                                                     tCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGaa                                                                          <  2:389152/148‑1 (MQ=255)
                                                                       cccTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCGTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagc                                                                        >  2:177741/1‑148 (MQ=255)
                                                                            aaTATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCaa                                                                   >  2:2554735/1‑148 (MQ=255)
                                                                             atatCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCaaa                                                                  >  2:866783/1‑148 (MQ=255)
                                                                             atatCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCaaa                                                                  >  2:2099216/1‑148 (MQ=255)
                                                                              tatCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAg                                                                 >  1:1570059/1‑148 (MQ=255)
                                                                              tatCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAg                                                                 >  2:768547/1‑148 (MQ=255)
                                                                              tatCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAg                                                                 >  2:2552095/1‑148 (MQ=255)
                                                                               atCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGc                                                                >  2:2967042/1‑148 (MQ=255)
                                                                                  aaGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCga                                                             >  2:1486669/1‑148 (MQ=255)
                                                                                   aGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                            <  1:521668/148‑1 (MQ=255)
                                                                                   aGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                            <  1:2150205/148‑1 (MQ=255)
                                                                                   aGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                            <  2:53203/148‑1 (MQ=255)
                                                                                    gCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaa                                                           >  2:1352000/1‑148 (MQ=255)
                                                                                     cATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaag                                                          >  1:974462/1‑148 (MQ=255)
                                                                                       ttCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                        >  2:3137536/1‑148 (MQ=255)
                                                                                       ttCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                        >  2:3137510/1‑148 (MQ=255)
                                                                                       gtCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                        <  2:204183/147‑1 (MQ=255)
                                                                                       gtCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                        <  2:1999554/147‑1 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                       >  1:430785/1‑148 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                       >  1:2818700/1‑148 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                       >  1:2365768/1‑148 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                       >  1:1041428/1‑148 (MQ=255)
                                                                                            tAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAcc                                                   <  1:379284/148‑1 (MQ=255)
                                                                                               aTTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATa                                                >  2:2180212/1‑148 (MQ=255)
                                                                                                 ttACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAcc                                              >  1:2418438/1‑148 (MQ=255)
                                                                                                  tACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCg                                             >  1:540793/1‑148 (MQ=255)
                                                                                                     aGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGTGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCt                                          <  2:718054/148‑1 (MQ=255)
                                                                                                     aGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCt                                          <  2:2680312/148‑1 (MQ=255)
                                                                                                      gTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCtt                                         <  1:953887/148‑1 (MQ=255)
                                                                                                      gTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCtt                                         <  1:776537/148‑1 (MQ=255)
                                                                                                      gTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCtt                                         <  1:1634400/148‑1 (MQ=255)
                                                                                                       ttCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTtc                                        >  1:1188214/1‑148 (MQ=255)
                                                                                                           tCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGa                                    <  1:1971832/148‑1 (MQ=255)
                                                                                                             aTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATg                                  <  1:1445423/148‑1 (MQ=255)
                                                                                                             aTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATg                                  <  1:586194/148‑1 (MQ=255)
                                                                                                              tGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATgg                                 <  2:1454938/148‑1 (MQ=255)
                                                                                                                 ttCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTaa                              <  1:1435670/148‑1 (MQ=255)
                                                                                                                 ttCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTaa                              <  2:455792/148‑1 (MQ=255)
                                                                                                                   cGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTGACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATg                            <  1:2684299/148‑1 (MQ=255)
                                                                                                                    gCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATgg                           <  1:1278895/148‑1 (MQ=255)
                                                                                                                           aTCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAc                    >  2:3519781/1‑148 (MQ=255)
                                                                                                                              gTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCtt                 <  2:2567237/148‑1 (MQ=255)
                                                                                                                               ttGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCttt                >  2:416095/1‑148 (MQ=255)
                                                                                                                                tGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTa               <  1:1169856/148‑1 (MQ=255)
                                                                                                                                tGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTa               >  2:1606472/1‑148 (MQ=255)
                                                                                                                                   cAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGt            >  2:3478563/1‑148 (MQ=255)
                                                                                                                                   cAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGt            >  2:2209029/1‑148 (MQ=255)
                                                                                                                                     gCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTcc          <  1:3348331/148‑1 (MQ=255)
                                                                                                                                          aCTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgtg     >  2:317310/1‑148 (MQ=255)
                                                                                                                                          aCTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgtg     <  2:362326/148‑1 (MQ=255)
                                                                                                                                             ccATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCa  >  2:1775302/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCA  >  CP009273/2459831‑2460119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  CP009273/2459827‑2460125
                                                                                                                                                     |                                                                                                                                                     
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATT                                                                                                                                                       <  SRR19973067.3141157/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     <  SRR19973067.2474375/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACAATTTATCTGTTCTCTCCCATCCGTACCAGTCCGCAAAATCCCCTGTATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     <  SRR19973067.2520724/150‑1 (MQ=60)
    AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGG                                                                                                                                                   <  SRR19973067.1743710/150‑1 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                          <  SRR19973067.3406497/150‑1 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                          <  SRR19973067.474185/150‑1 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                          <  SRR19973067.474228/150‑1 (MQ=60)
              TCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTG                                                                                                                                         <  SRR19973067.2975714/150‑1 (MQ=60)
               CGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGG                                                                                                                                        >  SRR19973067.94252/1‑150 (MQ=60)
               CGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGG                                                                                                                                        >  SRR19973067.94254/1‑150 (MQ=60)
                GATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGA                                                                                                                                       <  SRR19973067.3490077/150‑1 (MQ=60)
                GATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGA                                                                                                                                       <  SRR19973067.355830/150‑1 (MQ=60)
                 ATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGAT                                                                                                                                      <  SRR19973067.910384/150‑1 (MQ=60)
                   TCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAA                                                                                                                                    <  SRR19973067.932502/150‑1 (MQ=60)
                   TCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAA                                                                                                                                    <  SRR19973067.3498180/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                                  <  SRR19973067.1363764/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                                  <  SRR19973067.346151/150‑1 (MQ=60)
                       GCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATA                                                                                                                                <  SRR19973067.2484696/150‑1 (MQ=60)
                       GCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATA                                                                                                                                <  SRR19973067.2484245/150‑1 (MQ=60)
                       GCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATA                                                                                                                                <  SRR19973067.282685/150‑1 (MQ=60)
                       GCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATA                                                                                                                                <  SRR19973067.2090604/150‑1 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAT                                                                                                                              >  SRR19973067.1742777/1‑150 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAT                                                                                                                              >  SRR19973067.2068632/1‑150 (MQ=60)
                          GGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATT                                                                                                                             <  SRR19973067.1700666/150‑1 (MQ=60)
                           GGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTT                                                                                                                            <  SRR19973067.12146/150‑1 (MQ=60)
                             GACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTA                                                                                                                          <  SRR19973067.2497467/150‑1 (MQ=60)
                             GACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTA                                                                                                                          <  SRR19973067.3165480/150‑1 (MQ=60)
                               CACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACC                                                                                                                        >  SRR19973067.3081754/1‑150 (MQ=60)
                               CACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACC                                                                                                                        >  SRR19973067.2216518/1‑150 (MQ=60)
                               CACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACC                                                                                                                        >  SRR19973067.2217186/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                       >  SRR19973067.862226/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                       >  SRR19973067.2653890/1‑150 (MQ=60)
                                    TTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCG                                                                                                                   <  SRR19973067.2581993/150‑1 (MQ=60)
                                    TTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCG                                                                                                                   <  SRR19973067.1639258/150‑1 (MQ=60)
                                           GTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTT                                                                                                            >  SRR19973067.23698/1‑150 (MQ=60)
                                            TTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTA                                                                                                           >  SRR19973067.2448612/1‑150 (MQ=60)
                                             TCTCTCCGATCCGTACCAGTCCGCTTAATGCGCTGAATATAAAGCATTACTTAGATTTTCTGTTCGTAATGGTTCTCTTAAGATCGTTGTCTGCGGCACTCGATTACGGGTTAAAATTGTATAAAATAATTTTACCCACCGGATTTTTAC                                                                                                          <  SRR19973067.2629037/150‑1 (MQ=27)
                                              CGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACC                                                                                                         >  SRR19973067.2551410/1‑150 (MQ=60)
                                                  CCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGATAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATG                                                                                                     >  SRR19973067.3166687/1‑150 (MQ=60)
                                                    CATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCT                                                                                                   <  SRR19973067.2833817/150‑1 (MQ=60)
                                                    CATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCCTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCT                                                                                                   <  SRR19973067.2291791/150‑1 (MQ=60)
                                                        CGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACC                                                                                               >  SRR19973067.1103382/1‑150 (MQ=60)
                                                        CGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACC                                                                                               >  SRR19973067.1103265/1‑150 (MQ=60)
                                                           ACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTT                                                                                            >  SRR19973067.3093767/1‑150 (MQ=60)
                                                            CCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTA                                                                                           <  SRR19973067.3403606/150‑1 (MQ=60)
                                                              AGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAG                                                                                         >  SRR19973067.1511176/1‑150 (MQ=60)
                                                              AGTCCGAAAAATCCCCTTAATATCAAGCATTTCGTAGATTTTCTGTTAGTCATGGTTCTCTTCTGATCGTTGACAGACGCACTCCATTACGGTTAAAATTTGGATAAAATAATTTTACCCACGGGATTTTTACCCATGCTCACCGTTATG                                                                                         <  SRR19973067.2486568/150‑1 (MQ=60)
                                                                     AAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTG                                                                                  >  SRR19973067.314767/1‑150 (MQ=60)
                                                                      AAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTGAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGA                                                                                 >  SRR19973067.1848797/1‑150 (MQ=60)
                                                                      AAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTGAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGA                                                                                 >  SRR19973067.1848780/1‑150 (MQ=60)
                                                                       AATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAA                                                                                <  SRR19973067.980031/150‑1 (MQ=60)
                                                                        ATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCNATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAG                                                                               <  SRR19973067.1813896/150‑1 (MQ=60)
                                                                        ATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAG                                                                               >  SRR19973067.387349/1‑150 (MQ=60)
                                                                                AATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAG                                                                       >  SRR19973067.1580803/1‑150 (MQ=60)
                                                                                AATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCAACCGGATTTTTAAACATGCTCACCGTTACGCAGATTGTATCAGCAAAG                                                                       >  SRR19973067.1580782/1‑150 (MQ=60)
                                                                                     CAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACAGGATTTTTACCCATGCTCACCGTTAATCAGATTGAAGCAGCAAAGCCGAA                                                                  >  SRR19973067.2267798/1‑150 (MQ=60)
                                                                                       AGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAG                                                                <  SRR19973067.525192/150‑1 (MQ=60)
                                                                                       AGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAG                                                                >  SRR19973067.981141/1‑150 (MQ=60)
                                                                                       AGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAG                                                                <  SRR19973067.2164576/150‑1 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                             >  SRR19973067.1048588/1‑150 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                             >  SRR19973067.433781/1‑150 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                             >  SRR19973067.2836847/1‑150 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                             >  SRR19973067.2381174/1‑150 (MQ=60)
                                                                                                TAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCAT                                                       <  SRR19973067.381922/150‑1 (MQ=60)
                                                                                                   ATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACC                                                    >  SRR19973067.2434282/1‑150 (MQ=60)
                                                                                                    TTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCG                                                   >  SRR19973067.544389/1‑150 (MQ=60)
                                                                                                    TTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCG                                                   >  SRR19973067.544787/1‑150 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               >  SRR19973067.2073431/1‑150 (MQ=60)
                                                                                                         AGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTC                                              >  SRR19973067.1196035/1‑150 (MQ=60)
                                                                                                         AGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTC                                              >  SRR19973067.2978375/1‑150 (MQ=60)
                                                                                                          GTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCT                                             <  SRR19973067.960402/150‑1 (MQ=60)
                                                                                                          GTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCT                                             <  SRR19973067.781919/150‑1 (MQ=60)
                                                                                                          GTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCT                                             <  SRR19973067.960420/150‑1 (MQ=60)
                                                                                                          GTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCT                                             <  SRR19973067.1645642/150‑1 (MQ=60)
                                                                                                               TCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATG                                        <  SRR19973067.1985202/150‑1 (MQ=60)
                                                                                                                CATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGG                                       <  SRR19973067.1194460/150‑1 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                      <  SRR19973067.589939/150‑1 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                      <  SRR19973067.1455126/150‑1 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                      <  SRR19973067.1455159/150‑1 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                      <  SRR19973067.590593/150‑1 (MQ=60)
                                                                                                                    GTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAAT                                   <  SRR19973067.2395458/150‑1 (MQ=60)
                                                                                                                     TTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATG                                  <  SRR19973067.1445337/150‑1 (MQ=60)
                                                                                                                       CGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTGACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGC                                <  SRR19973067.2701509/150‑1 (MQ=60)
                                                                                                                        GCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCC                               <  SRR19973067.1287607/150‑1 (MQ=60)
                                                                                                                        GCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCC                               <  SRR19973067.2869178/150‑1 (MQ=60)
                                                                                                                             AGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAC                          <  SRR19973067.2782938/150‑1 (MQ=60)
                                                                                                                                    TGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATG                   <  SRR19973067.1177615/150‑1 (MQ=60)
                                                                                                                                         GCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCT              <  SRR19973067.3369558/150‑1 (MQ=60)
                                                                                                                                              ACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTC         >  SRR19973067.2067285/1‑150 (MQ=60)
                                                                                                                                               CTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCA        <  SRR19973067.451766/150‑1 (MQ=60)
                                                                                                                                                 CCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGG      >  SRR19973067.2456439/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973067.347969/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973067.2922421/1‑150 (MQ=60)
                                                                                                                                                     TACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  SRR19973067.1078168/1‑150 (MQ=60)
                                                                                                                                                     GACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  SRR19973067.2472541/1‑150 (MQ=60)
                                                                                                                                                     GACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  SRR19973067.1005387/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  CP009273/2459827‑2460125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑