Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,459,976 G→T 100% intergenic (+114/‑48) argW → / → intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,459,9760GT96.3% 647.7 / 14.8 214intergenic (+114/‑48)argW/intStRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base T (98/108);  total (101/113)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAG  >  CP009273/2459831‑2460120
                                                                                                                                                 |                                                                                                                                                
aaTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAc                                                                                                                                                >  1:2406032/1‑148 (MQ=255)
 aTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACg                                                                                                                                               <  1:1143255/148‑1 (MQ=255)
 aTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACg                                                                                                                                               <  2:1583778/148‑1 (MQ=255)
  ttGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACgg                                                                                                                                              <  1:1336652/148‑1 (MQ=255)
  ttGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACgg                                                                                                                                              <  2:2381531/148‑1 (MQ=255)
       ggTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaa                                                                                                                                         >  1:3144042/1‑148 (MQ=255)
        gTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaa                                                                                                                                        <  1:2216174/148‑1 (MQ=255)
        gTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaa                                                                                                                                        <  2:1781878/148‑1 (MQ=255)
         ttCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaaa                                                                                                                                       >  2:1983744/1‑148 (MQ=255)
            gATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTg                                                                                                                                    <  1:2050746/148‑1 (MQ=255)
              ttCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGa                                                                                                                                  >  1:555995/1‑148 (MQ=255)
                 cTGCAGGGGACACAATATATCAGTTCTCTCCCATCCGTACCACTCCGCAAAATCCCCTGAATATCAAGCATTCAGTAGATGTACAGTTCTTCCTGGTTCTCTTCAGATCTTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaa                                                                                                                               <  2:3097581/148‑1 (MQ=255)
                  tGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaa                                                                                                                              <  2:2091134/148‑1 (MQ=255)
                   gCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaaaa                                                                                                                             <  2:1699516/148‑1 (MQ=255)
                     aGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaata                                                                                                                           <  1:3303842/148‑1 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGGTAAaataat                                                                                                                         >  1:733980/1‑148 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                         >  2:3119032/1‑148 (MQ=255)
                           cacCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTa                                                                                                                     <  2:2658830/148‑1 (MQ=255)
                              cATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAccc                                                                                                                  >  1:1656008/1‑148 (MQ=255)
                                   aTCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTTCCCACCgg                                                                                                             >  2:3259639/1‑148 (MQ=255)
                                   aTCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCgg                                                                                                             >  2:3137100/1‑148 (MQ=255)
                                   aTCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCgg                                                                                                             <  2:3024169/148‑1 (MQ=255)
                                    tCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGa                                                                                                            <  2:3316565/148‑1 (MQ=255)
                                    tCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGa                                                                                                            >  1:288977/1‑148 (MQ=255)
                                     cAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAt                                                                                                           <  2:533002/148‑1 (MQ=255)
                                      aGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTATATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAtt                                                                                                          >  1:3222654/1‑148 (MQ=255)
                                           gCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCNCTTCAGAGCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTAc                                                                                                     >  1:1786005/1‑148 (MQ=255)
                                           gCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTAc                                                                                                     <  2:3250336/148‑1 (MQ=255)
                                             tCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTAccc                                                                                                   <  2:1776821/148‑1 (MQ=255)
                                                cATCCGTACCAGTCCGCAAACTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATg                                                                                                <  1:1874437/148‑1 (MQ=255)
                                                  tCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCACACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCt                                                                                              >  2:865859/1‑148 (MQ=255)
                                                   ccGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTc                                                                                             >  1:432052/1‑148 (MQ=255)
                                                   ccGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTc                                                                                             >  1:1296340/1‑148 (MQ=255)
                                                   ccGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTc                                                                                             <  2:1226904/148‑1 (MQ=255)
                                                    cGTACCAGTCCTCAAAATCCCCTGAATATCCAGCATTCCGTAGATTTACAGTTCGTCATTGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCa                                                                                            <  1:3379756/148‑1 (MQ=255)
                                                     gTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAc                                                                                           <  2:3409305/148‑1 (MQ=255)
                                                       aCCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCg                                                                                         >  2:213/1‑148 (MQ=255)
                                                       aCCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCg                                                                                         >  2:242/1‑148 (MQ=255)
                                                       aCCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCg                                                                                         >  2:3066381/1‑148 (MQ=255)
                                                       aCCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCg                                                                                         <  1:2088919/148‑1 (MQ=255)
                                                           gTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTaa                                                                                     >  1:2817337/1‑148 (MQ=255)
                                                            tCCGCAAAATCCCCTGAATATCCCGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAg                                                                                    <  1:786039/148‑1 (MQ=255)
                                                            tCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAg                                                                                    >  2:2501253/1‑148 (MQ=255)
                                                             ccGCAAAATCCCCTGAATCTCAAGCATTCCGTAGATTTACAGTTCGTCTTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGc                                                                                   <  2:509844/148‑1 (MQ=255)
                                                              cGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCa                                                                                  <  1:1077104/148‑1 (MQ=255)
                                                               gCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAg                                                                                 >  2:1302411/1‑148 (MQ=255)
                                                                  aaaTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAtt                                                                              >  1:8583/1‑148 (MQ=255)
                                                                  aaaTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTAGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAtt                                                                              <  1:1838469/148‑1 (MQ=255)
                                                                   aaTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTg                                                                             <  1:2172571/148‑1 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                            <  2:2504074/148‑1 (MQ=255)
                                                                     tCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGaa                                                                           >  2:1881405/1‑148 (MQ=255)
                                                                      ccccTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGTTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAag                                                                          <  2:2796254/148‑1 (MQ=255)
                                                                      ccccTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAag                                                                          <  2:2883825/148‑1 (MQ=255)
                                                                       cccTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagc                                                                         <  1:1003454/148‑1 (MQ=255)
                                                                         cTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcag                                                                       >  2:1557062/1‑148 (MQ=255)
                                                                          tGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcagc                                                                      >  1:888023/1‑148 (MQ=255)
                                                                          tGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcagc                                                                      >  2:750571/1‑148 (MQ=255)
                                                                          tGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcagc                                                                      >  2:174063/1‑148 (MQ=255)
                                                                           gAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAgaagca                                                                     >  2:3479566/1‑148 (MQ=255)
                                                                                   aGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                             <  1:1587561/148‑1 (MQ=255)
                                                                                     cATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaag                                                           <  2:1380828/148‑1 (MQ=255)
                                                                                     cATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaag                                                           >  2:1948976/1‑148 (MQ=255)
                                                                                      aTTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaaga                                                          >  1:540625/1‑148 (MQ=255)
                                                                                      aTTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaaga                                                          <  1:3098109/148‑1 (MQ=255)
                                                                                         ccGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGaaaa                                                       >  1:2160569/1‑148 (MQ=255)
                                                                                            tAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGGTAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAcc                                                    <  2:733980/148‑1 (MQ=255)
                                                                                            tAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAcc                                                    >  1:513276/1‑148 (MQ=255)
                                                                                            tAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAcc                                                    <  2:268674/148‑1 (MQ=255)
                                                                                            tAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAcc                                                    >  2:3179330/1‑148 (MQ=255)
                                                                                             aGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCa                                                   >  1:2977907/1‑148 (MQ=255)
                                                                                             aGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCa                                                   >  1:97933/1‑148 (MQ=255)
                                                                                                 ttACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAcc                                               >  1:2480163/1‑148 (MQ=255)
                                                                                                    cAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGcc                                            <  2:1376249/148‑1 (MQ=255)
                                                                                                    cAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGcc                                            <  1:210741/148‑1 (MQ=255)
                                                                                                      gTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCtt                                          >  1:1190636/1‑148 (MQ=255)
                                                                                                      gTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCtt                                          >  1:1808893/1‑148 (MQ=255)
                                                                                                           tCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGa                                     <  1:1732484/148‑1 (MQ=255)
                                                                                                           tCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGa                                     >  2:2816593/1‑148 (MQ=255)
                                                                                                             aTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATg                                   >  2:2349330/1‑148 (MQ=255)
                                                                                                              tGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATgg                                  <  2:1639341/148‑1 (MQ=255)
                                                                                                               ggTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGt                                 <  1:372662/148‑1 (MQ=255)
                                                                                                               ggTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGt                                 >  2:1173071/1‑148 (MQ=255)
                                                                                                                gTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTa                                <  2:3013752/148‑1 (MQ=255)
                                                                                                                gTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTa                                <  2:2294624/148‑1 (MQ=255)
                                                                                                                 ttCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTaa                               >  1:3171177/1‑148 (MQ=255)
                                                                                                                   cGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATg                             <  2:615582/148‑1 (MQ=255)
                                                                                                                          gATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTa                      >  1:533864/1‑148 (MQ=255)
                                                                                                                           aTCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAc                     >  2:2840735/1‑148 (MQ=255)
                                                                                                                           aTCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGAGGGTAATGGCCTGTAc                     >  2:2500944/1‑148 (MQ=255)
                                                                                                                                  aCAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATg              <  1:255029/148‑1 (MQ=255)
                                                                                                                                  aCAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATg              <  1:2501276/148‑1 (MQ=255)
                                                                                                                                   cAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGt             <  2:560239/148‑1 (MQ=255)
                                                                                                                                     gCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTcc           <  1:1948976/148‑1 (MQ=255)
                                                                                                                                      ccGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTccc          <  1:2424450/148‑1 (MQ=255)
                                                                                                                                      ccGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTccc          <  1:661718/148‑1 (MQ=255)
                                                                                                                                      ccGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTccc          >  2:1674539/1‑148 (MQ=255)
                                                                                                                                      ccGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTccc          <  1:1985078/148‑1 (MQ=255)
                                                                                                                                           cTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTgtgt     <  2:3421689/148‑1 (MQ=255)
                                                                                                                                              cATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAg  <  1:1202341/148‑1 (MQ=255)
                                                                                                                                              cATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAg  <  2:3171153/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAG  >  CP009273/2459831‑2460120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  CP009273/2459827‑2460125
                                                                                                                                                     |                                                                                                                                                     
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATT                                                                                                                                                       >  SRR19973065.563806/1‑150 (MQ=60)
 GCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTA                                                                                                                                                      >  SRR19973065.1897661/1‑150 (MQ=60)
 GCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTA                                                                                                                                                      <  SRR19973065.1993487/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     <  SRR19973065.3470495/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     >  SRR19973065.2421169/1‑150 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     <  SRR19973065.3470401/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                     <  SRR19973065.3156372/150‑1 (MQ=60)
    AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCCAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGG                                                                                                                                                   <  SRR19973065.419696/150‑1 (MQ=60)
     ATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGG                                                                                                                                                  <  SRR19973065.1150637/150‑1 (MQ=60)
      TTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGT                                                                                                                                                 <  SRR19973065.1345506/150‑1 (MQ=60)
         CAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAA                                                                                                                                              >  SRR19973065.3412146/1‑150 (MQ=60)
         CAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAA                                                                                                                                              >  SRR19973065.3163142/1‑150 (MQ=60)
            GTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAG                                                                                                                                           <  SRR19973065.2230328/150‑1 (MQ=60)
            GTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAG                                                                                                                                           <  SRR19973065.2182347/150‑1 (MQ=60)
            GTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAG                                                                                                                                           <  SRR19973065.2942711/150‑1 (MQ=60)
            GTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAG                                                                                                                                           >  SRR19973065.374577/1‑150 (MQ=60)
                GATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGA                                                                                                                                       >  SRR19973065.559547/1‑150 (MQ=60)
                GATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGA                                                                                                                                       <  SRR19973065.2063846/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCAACCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGAAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                                  >  SRR19973065.3024600/1‑150 (MQ=60)
                      TGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAAT                                                                                                                                 <  SRR19973065.3157030/150‑1 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGGTAAAATAAT                                                                                                                              >  SRR19973065.738891/1‑150 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAT                                                                                                                              <  SRR19973065.3236688/150‑1 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAT                                                                                                                              <  SRR19973065.3323966/150‑1 (MQ=60)
                          GGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATT                                                                                                                             >  SRR19973065.2416605/1‑150 (MQ=60)
                           GGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTT                                                                                                                            <  SRR19973065.3288598/150‑1 (MQ=60)
                           GGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTT                                                                                                                            >  SRR19973065.2519813/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                       <  SRR19973065.1329169/150‑1 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                       <  SRR19973065.51663/150‑1 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                       >  SRR19973065.1667109/1‑150 (MQ=60)
                                 CCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCCAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCA                                                                                                                      <  SRR19973065.1140638/150‑1 (MQ=60)
                                 CCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCA                                                                                                                      >  SRR19973065.2309080/1‑150 (MQ=60)
                                  CATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAC                                                                                                                     >  SRR19973065.2116463/1‑150 (MQ=60)
                                      TATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGA                                                                                                                 >  SRR19973065.290864/1‑150 (MQ=60)
                                        TCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTATATTTACAGTTCGTCATGGTTCGCTTCAGATAGTTGACAGCCGCACTCCAATACGGGTAAAAAGTGGATAAAATAATGTTACCCACCGGATT                                                                                                               >  SRR19973065.3242014/1‑150 (MQ=60)
                                         CAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTT                                                                                                              <  SRR19973065.1651801/150‑1 (MQ=60)
                                          AGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTTCCCACCGGATTTT                                                                                                             <  SRR19973065.635910/150‑1 (MQ=60)
                                          AGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTT                                                                                                             <  SRR19973065.2432162/150‑1 (MQ=60)
                                            TTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGGAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTA                                                                                                           >  SRR19973065.2483000/1‑150 (MQ=60)
                                             TCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCNCTTCAGAGCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTAC                                                                                                          >  SRR19973065.1797692/1‑150 (MQ=60)
                                                CTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCA                                                                                                       >  SRR19973065.525298/1‑150 (MQ=60)
                                                   CCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGC                                                                                                    >  SRR19973065.1186544/1‑150 (MQ=60)
                                                    CATCCGTACCAGTCCGCAAACTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCT                                                                                                   <  SRR19973065.1886779/150‑1 (MQ=60)
                                                    CATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCT                                                                                                   <  SRR19973065.1886778/150‑1 (MQ=60)
                                                     ATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTC                                                                                                  >  SRR19973065.435056/1‑150 (MQ=60)
                                                     ATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTC                                                                                                  >  SRR19973065.1304880/1‑150 (MQ=60)
                                                       CCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAC                                                                                                >  SRR19973065.778367/1‑150 (MQ=60)
                                                        CGTACCAGTCCTCAAAATCCCCTGAATATCCAGCATTCCGTAGATTTACAGTTCGTCATTGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACC                                                                                               <  SRR19973065.3400420/150‑1 (MQ=60)
                                                        CGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACC                                                                                               >  SRR19973065.1492501/1‑150 (MQ=60)
                                                           ACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTT                                                                                            <  SRR19973065.2102324/150‑1 (MQ=60)
                                                             CAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAA                                                                                          >  SRR19973065.2834745/1‑150 (MQ=60)
                                                                TCCGCAAAATCCCCTGAATATCCCGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCA                                                                                       <  SRR19973065.791250/150‑1 (MQ=60)
                                                                  CGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGA                                                                                     >  SRR19973065.1728773/1‑150 (MQ=60)
                                                                  CGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGA                                                                                     >  SRR19973065.2897161/1‑150 (MQ=60)
                                                                  CGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGA                                                                                     <  SRR19973065.1084221/150‑1 (MQ=60)
                                                                    CAAAATCCCCTGAATATCCAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATT                                                                                   <  SRR19973065.733898/150‑1 (MQ=60)
                                                                    CAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATT                                                                                   >  SRR19973065.8719/1‑150 (MQ=60)
                                                                    CAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATT                                                                                   >  SRR19973065.8211/1‑150 (MQ=60)
                                                                    CAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTACTCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAATTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGAAGATT                                                                                   >  SRR19973065.1799806/1‑150 (MQ=60)
                                                                      AAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTAGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGA                                                                                 <  SRR19973065.1850590/150‑1 (MQ=60)
                                                                       AATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAA                                                                                <  SRR19973065.2186513/150‑1 (MQ=60)
                                                                           CCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAG                                                                            <  SRR19973065.1010118/150‑1 (MQ=60)
                                                                            CCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGC                                                                           >  SRR19973065.893649/1‑150 (MQ=60)
                                                                            CCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGC                                                                           >  SRR19973065.893661/1‑150 (MQ=60)
                                                                              TGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAA                                                                         <  SRR19973065.1779872/150‑1 (MQ=60)
                                                                                AATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAG                                                                       <  SRR19973065.2139273/150‑1 (MQ=60)
                                                                                       AGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAG                                                                <  SRR19973065.1598157/150‑1 (MQ=60)
                                                                                        GCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGA                                                               >  SRR19973065.543758/1‑150 (MQ=60)
                                                                                        GCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGA                                                               >  SRR19973065.544134/1‑150 (MQ=60)
                                                                                        GCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGA                                                               >  SRR19973065.544162/1‑150 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                             <  SRR19973065.3117026/150‑1 (MQ=60)
                                                                                           TTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAA                                                            >  SRR19973065.2174462/1‑150 (MQ=60)
                                                                                             CCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAC                                                          <  SRR19973065.105887/150‑1 (MQ=60)
                                                                                              CGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACC                                                         >  SRR19973065.516673/1‑150 (MQ=60)
                                                                                               GTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCA                                                        >  SRR19973065.2996128/1‑150 (MQ=60)
                                                                                               GTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCA                                                        >  SRR19973065.98835/1‑150 (MQ=60)
                                                                                                   ATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACC                                                    >  SRR19973065.2068250/1‑150 (MQ=60)
                                                                                                   ATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACC                                                    >  SRR19973065.2495777/1‑150 (MQ=60)
                                                                                                   ATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACC                                                    <  SRR19973065.3369923/150‑1 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               <  SRR19973065.212339/150‑1 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               <  SRR19973065.1996627/150‑1 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               >  SRR19973065.1198178/1‑150 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               >  SRR19973065.1820796/1‑150 (MQ=60)
                                                                                                        CAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTT                                               >  SRR19973065.1820804/1‑150 (MQ=60)
                                                                                                            TCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCG                                           <  SRR19973065.2986875/150‑1 (MQ=60)
                                                                                                               TCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATG                                        <  SRR19973065.1743949/150‑1 (MQ=60)
                                                                                                               TCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATG                                        <  SRR19973065.69580/150‑1 (MQ=60)
                                                                                                                CATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGG                                       <  SRR19973065.950555/150‑1 (MQ=60)
                                                                                                                   GGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAA                                    >  SRR19973065.3190364/1‑150 (MQ=60)
                                                                                                                   GGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAA                                    >  SRR19973065.3190340/1‑150 (MQ=60)
                                                                                                                   GGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAA                                    <  SRR19973065.375269/150‑1 (MQ=60)
                                                                                                                            CAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTA                           >  SRR19973065.537346/1‑150 (MQ=60)
                                                                                                                                TCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTT                       <  SRR19973065.1291917/150‑1 (MQ=60)
                                                                                                                                TCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTT                       <  SRR19973065.1660739/150‑1 (MQ=60)
                                                                                                                                 CGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTT                      <  SRR19973065.201965/150‑1 (MQ=60)
                                                                                                                                   TTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTAT                    <  SRR19973065.2211068/150‑1 (MQ=60)
                                                                                                                                      ACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTC                 <  SRR19973065.2516978/150‑1 (MQ=60)
                                                                                                                                      ACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTC                 <  SRR19973065.2517001/150‑1 (MQ=60)
                                                                                                                                      ACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTC                 <  SRR19973065.256809/150‑1 (MQ=60)
                                                                                                                                         GCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCT              <  SRR19973065.1961858/150‑1 (MQ=60)
                                                                                                                                         GCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCT              <  SRR19973065.1961714/150‑1 (MQ=60)
                                                                                                                                          CCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTG             <  SRR19973065.2439744/150‑1 (MQ=60)
                                                                                                                                          CCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTG             <  SRR19973065.260779/150‑1 (MQ=60)
                                                                                                                                          CCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTG             <  SRR19973065.666099/150‑1 (MQ=60)
                                                                                                                                          CCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTG             <  SRR19973065.1997964/150‑1 (MQ=60)
                                                                                                                                              ACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTC         >  SRR19973065.1908779/1‑150 (MQ=60)
                                                                                                                                                 CCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGG      >  SRR19973065.1187987/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973065.9931/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     <  SRR19973065.1209990/150‑1 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973065.9898/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     <  SRR19973065.1210049/150‑1 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973065.1230243/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973065.318300/1‑150 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGAGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG     >  SRR19973065.318236/1‑150 (MQ=60)
                                                                                                                                                   ATTACGGGTAAAAAGTGGATACAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGA    >  SRR19973065.3107694/1‑150 (MQ=60)
                                                                                                                                                   ATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGA    >  SRR19973065.1103388/1‑150 (MQ=60)
                                                                                                                                                   ATTACGGGTAAAAAGTGGATAAAATAATTTTACACACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCACTGTGTCAGGGA    >  SRR19973065.914190/1‑150 (MQ=60)
                                                                                                                                                    TTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAA   >  SRR19973065.3148819/1‑150 (MQ=60)
                                                                                                                                                     TACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  <  SRR19973065.2684891/150‑1 (MQ=60)
                                                                                                                                                     TACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  <  SRR19973065.2684960/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGGAAA  >  CP009273/2459827‑2460125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑