Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,459,976 G→T 100% intergenic (+114/‑48) argW → / → intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,459,9760GT95.8% 715.2 / 18.8 237intergenic (+114/‑48)argW/intStRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (114/112);  total (120/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAG  >  CP009273/2459831‑2460120
                                                                                                                                                 |                                                                                                                                                
aaTTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAc                                                                                                                                                >  2:2548913/1‑148 (MQ=255)
   tGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACggg                                                                                                                                             <  1:767563/148‑1 (MQ=255)
    gCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGt                                                                                                                                            <  1:633228/148‑1 (MQ=255)
         ttCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTaaaaa                                                                                                                                       <  2:1397104/148‑1 (MQ=255)
           cGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGt                                                                                                                                     >  1:2978104/1‑148 (MQ=255)
           cGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGt                                                                                                                                     >  1:2147736/1‑148 (MQ=255)
             aTTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTgg                                                                                                                                   >  2:2137792/1‑148 (MQ=255)
              ttCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGa                                                                                                                                  >  2:2654714/1‑148 (MQ=255)
              ttCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGa                                                                                                                                  <  2:677957/148‑1 (MQ=255)
                 cTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATaa                                                                                                                               <  1:3258865/148‑1 (MQ=255)
                      ggggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataa                                                                                                                          <  2:3159780/148‑1 (MQ=255)
                      ggggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAaataa                                                                                                                          >  1:3102828/1‑148 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                         >  2:291484/1‑148 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                         >  1:2353543/1‑148 (MQ=255)
                       gggACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAaataat                                                                                                                         >  2:409067/1‑148 (MQ=255)
                           cacCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATACCGTAGATTTACAGTTCGTCATGGTTCGATTAAGATCGTTGACACCCGCACTCCATTACGGGTAAAAAGTTGATAAAATAATATTa                                                                                                                     >  2:2381871/1‑148 (MQ=255)
                            acCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAc                                                                                                                    >  2:1334641/1‑148 (MQ=255)
                              cATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAccc                                                                                                                  >  1:1267217/1‑148 (MQ=255)
                              cATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAccc                                                                                                                  >  2:1772909/1‑148 (MQ=255)
                                tttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAc                                                                                                                >  2:2682957/1‑148 (MQ=255)
                                tttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAc                                                                                                                <  2:3137126/148‑1 (MQ=255)
                                 ttATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAcc                                                                                                               >  2:3316518/1‑148 (MQ=255)
                                     cAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAt                                                                                                           >  2:3448866/1‑148 (MQ=255)
                                     cAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAt                                                                                                           >  2:2397301/1‑148 (MQ=255)
                                      aGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAtt                                                                                                          <  1:994333/148‑1 (MQ=255)
                                        ttCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAtttt                                                                                                        <  2:469755/148‑1 (MQ=255)
                                         tCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAttttt                                                                                                       <  2:1691871/148‑1 (MQ=255)
                                          cGCTCCCCTCCGTACCCGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTa                                                                                                      <  2:3433581/148‑1 (MQ=255)
                                            cTCCCATCCGTACAAGTCCGCAAAATCCCCTGAATATCAAGCATTCCTTAGATTTACAGTTCGTCATGGTACGCTTCAGATCGTTGACAGCCGCACTCCATAACGGGTAAAAAGTGGATAAAATAATTTAACCAACCGGATTTTTAcc                                                                                                    >  1:1600873/1‑148 (MQ=255)
                                             tCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTAccc                                                                                                   <  1:3209392/148‑1 (MQ=255)
                                              cccATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCa                                                                                                  <  2:1369539/148‑1 (MQ=255)
                                                 aTCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGc                                                                                               >  1:3290364/1‑148 (MQ=255)
                                                  tCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCt                                                                                              >  2:512074/1‑148 (MQ=255)
                                                   ccGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTc                                                                                             >  2:1121130/1‑148 (MQ=255)
                                                     gTACCAGTCCGCAAAATCCCCTGAATATCAAGCATCCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAc                                                                                           >  2:1209800/1‑148 (MQ=255)
                                                      tACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCAcc                                                                                          >  2:1082007/1‑148 (MQ=255)
                                                         cAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGtt                                                                                       >  2:1387822/1‑148 (MQ=255)
                                                          aGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTa                                                                                      <  1:126466/148‑1 (MQ=255)
                                                           gTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTaa                                                                                     >  1:1467683/1‑148 (MQ=255)
                                                             ccGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGc                                                                                   >  2:3207481/1‑148 (MQ=255)
                                                              cGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCa                                                                                  >  1:781354/1‑148 (MQ=255)
                                                               gCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAg                                                                                 <  1:2358132/148‑1 (MQ=255)
                                                                cAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGa                                                                                >  1:1041243/1‑148 (MQ=255)
                                                                cAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGa                                                                                <  2:1432612/148‑1 (MQ=255)
                                                                cAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGa                                                                                <  2:3243508/148‑1 (MQ=255)
                                                                 aaaaTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAt                                                                               <  1:2654642/148‑1 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTCAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                            >  2:177919/1‑148 (MQ=255)
                                                                    aTCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGa                                                                            >  1:520313/1‑148 (MQ=255)
                                                                       cccTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagc                                                                         >  2:3520918/1‑148 (MQ=255)
                                                                         cTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcag                                                                       >  1:2974937/1‑148 (MQ=255)
                                                                          tGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAagcagc                                                                      >  1:1634614/1‑148 (MQ=255)
                                                                           gAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAgcagca                                                                     <  1:1545629/148‑1 (MQ=255)
                                                                            aaTATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCaa                                                                    >  1:2852062/1‑148 (MQ=255)
                                                                            aaTATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCaa                                                                    <  1:1180035/148‑1 (MQ=255)
                                                                               atCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGc                                                                 >  1:3062798/1‑148 (MQ=255)
                                                                               atCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGAAAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGc                                                                 <  1:3468368/148‑1 (MQ=255)
                                                                                tCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGcc                                                                >  2:2808491/1‑148 (MQ=255)
                                                                                   aGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                             <  2:1113709/148‑1 (MQ=255)
                                                                                   aGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaa                                                             <  1:1308660/148‑1 (MQ=255)
                                                                                     cATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaag                                                           >  2:2410323/1‑148 (MQ=255)
                                                                                       ttCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                         <  1:1658296/148‑1 (MQ=255)
                                                                                       ttCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                         <  1:415384/148‑1 (MQ=255)
                                                                                       ttCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaa                                                         >  2:3093833/1‑148 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                        >  2:521922/1‑148 (MQ=255)
                                                                                        tCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCgaaagaaa                                                        >  1:662087/1‑148 (MQ=255)
                                                                                          cGTAGATGTACAGTTCGTCATGGTTCGCTACAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGCTTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGaaaaa                                                      <  1:2967502/148‑1 (MQ=255)
                                                                                                tttACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAc                                                >  2:1914992/1‑148 (MQ=255)
                                                                                                tttACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAc                                                >  1:1168515/1‑148 (MQ=255)
                                                                                                 ttACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAcc                                               >  2:3043616/1‑148 (MQ=255)
                                                                                                     aGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCt                                           >  1:1135381/1‑148 (MQ=255)
                                                                                                       ttCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTtc                                         >  2:2334645/1‑148 (MQ=255)
                                                                                                        tCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTtct                                        <  1:293425/148‑1 (MQ=255)
                                                                                                        tCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTtct                                        <  1:1711585/148‑1 (MQ=255)
                                                                                                            cATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGAt                                    >  1:3410754/1‑148 (MQ=255)
                                                                                                             aTGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATg                                   <  1:1087086/148‑1 (MQ=255)
                                                                                                              tGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATgg                                  <  1:3417681/148‑1 (MQ=255)
                                                                                                               ggTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGt                                 <  1:2011804/148‑1 (MQ=255)
                                                                                                                 ttCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTaa                               >  2:861499/1‑148 (MQ=255)
                                                                                                                   cGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATg                             >  1:857508/1‑148 (MQ=255)
                                                                                                                   cGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATg                             <  1:2389740/148‑1 (MQ=255)
                                                                                                                   cGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATg                             <  1:2592883/148‑1 (MQ=255)
                                                                                                                     cTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGc                           <  2:2725750/148‑1 (MQ=255)
                                                                                                                       tCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCt                         >  1:1923946/1‑148 (MQ=255)
                                                                                                                           aTCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAc                     <  2:2506019/148‑1 (MQ=255)
                                                                                                                            tCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAcc                    >  1:114592/1‑148 (MQ=255)
                                                                                                                            tCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTAcc                    <  2:781354/148‑1 (MQ=255)
                                                                                                                             cGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCt                   <  1:2685258/148‑1 (MQ=255)
                                                                                                                               ttGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCttt                 >  1:1342535/1‑148 (MQ=255)
                                                                                                                               ttGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCttt                 <  2:1664796/148‑1 (MQ=255)
                                                                                                                                tGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTa                <  2:379794/148‑1 (MQ=255)
                                                                                                                                  aCAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGNTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATg              >  1:1808529/1‑148 (MQ=255)
                                                                                                                                     gCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTcc           <  2:1636574/148‑1 (MQ=255)
                                                                                                                                      ccGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTccc          >  2:864307/1‑148 (MQ=255)
                                                                                                                                         cACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgt       >  1:3450542/1‑148 (MQ=255)
                                                                                                                                         cACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgt       >  1:935419/1‑148 (MQ=255)
                                                                                                                                         cACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgt       >  1:1966268/1‑148 (MQ=255)
                                                                                                                                         cACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTGAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCtgt       <  2:3464021/148‑1 (MQ=255)
                                                                                                                                           cTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTgtgt     <  2:3323126/148‑1 (MQ=255)
                                                                                                                                           cTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTgtgt     <  1:1108569/148‑1 (MQ=255)
                                                                                                                                             ccATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCa   >  2:89935/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAG  >  CP009273/2459831‑2460120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG  >  CP009273/2459827‑2460122
                                                                                                                                                     |                                                                                                                                                  
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATT                                                                                                                                                    <  SRR19973066.3114559/150‑1 (MQ=60)
  CTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTAC                                                                                                                                                  <  SRR19973066.73283/150‑1 (MQ=60)
       TGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTA                                                                                                                                             <  SRR19973066.3333868/150‑1 (MQ=60)
       TGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTA                                                                                                                                             <  SRR19973066.773250/150‑1 (MQ=60)
        GCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAA                                                                                                                                            <  SRR19973066.637786/150‑1 (MQ=60)
        GCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAA                                                                                                                                            <  SRR19973066.637790/150‑1 (MQ=60)
         CAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAA                                                                                                                                           <  SRR19973066.2844893/150‑1 (MQ=60)
         CAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCAGTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAA                                                                                                                                           <  SRR19973066.1052309/150‑1 (MQ=60)
          AGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAA                                                                                                                                          >  SRR19973066.275790/1‑150 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                       >  SRR19973066.2162641/1‑150 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                       >  SRR19973066.2997782/1‑150 (MQ=60)
             TTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGT                                                                                                                                       >  SRR19973066.2997650/1‑150 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                               <  SRR19973066.3280432/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                               <  SRR19973066.3279944/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                               <  SRR19973066.3280079/150‑1 (MQ=60)
                     CTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAA                                                                                                                               <  SRR19973066.3280303/150‑1 (MQ=60)
                       GCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATA                                                                                                                             <  SRR19973066.1564639/150‑1 (MQ=60)
                        CAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAA                                                                                                                            >  SRR19973066.3123553/1‑150 (MQ=60)
                        CAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAA                                                                                                                            >  SRR19973066.3123511/1‑150 (MQ=60)
                         AGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAAT                                                                                                                           >  SRR19973066.2369527/1‑150 (MQ=60)
                            GGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTT                                                                                                                        >  SRR19973066.1376185/1‑150 (MQ=60)
                              ACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAC                                                                                                                      <  SRR19973066.1734558/150‑1 (MQ=60)
                              ACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTAC                                                                                                                      <  SRR19973066.1734897/150‑1 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                    >  SRR19973066.1276368/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                    >  SRR19973066.1276224/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                    >  SRR19973066.183328/1‑150 (MQ=60)
                                ACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCAATTACGGGTAAAAAGTGGATAAAATAATTTTACCC                                                                                                                    >  SRR19973066.2472869/1‑150 (MQ=60)
                                  CATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCAC                                                                                                                  >  SRR19973066.1313145/1‑150 (MQ=60)
                                       ATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAAAATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGAT                                                                                                             >  SRR19973066.1437464/1‑150 (MQ=60)
                                         CAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTT                                                                                                           <  SRR19973066.988736/150‑1 (MQ=60)
                                          AGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTT                                                                                                          <  SRR19973066.2357195/150‑1 (MQ=60)
                                          AGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTT                                                                                                          <  SRR19973066.1001661/150‑1 (MQ=60)
                                           GTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTT                                                                                                         <  SRR19973066.1941666/150‑1 (MQ=60)
                                              CGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACC                                                                                                      >  SRR19973066.1613818/1‑150 (MQ=60)
                                              CGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACC                                                                                                      >  SRR19973066.2647388/1‑150 (MQ=60)
                                              CGCTCCCATCCGTACAAGTCCGCAAAATCCCCTGAATATCAAGCATTCCTTAGATTTACAGTTCGTCATGGTACGCTTCAGATCGTTGACAGCCGCACTCCATAACGGGTAAAAAGTGGATAAAATAATTTAACCAACCGGATTTTTACC                                                                                                      >  SRR19973066.1612488/1‑150 (MQ=60)
                                               GCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCC                                                                                                     <  SRR19973066.1719758/150‑1 (MQ=60)
                                                 TCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCAT                                                                                                   <  SRR19973066.3230670/150‑1 (MQ=60)
                                                 TCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCAT                                                                                                   <  SRR19973066.2437748/150‑1 (MQ=60)
                                                   CCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGC                                                                                                 >  SRR19973066.3312064/1‑150 (MQ=60)
                                                     ATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTC                                                                                               >  SRR19973066.3478597/1‑150 (MQ=60)
                                                        CGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACC                                                                                            >  SRR19973066.886013/1‑150 (MQ=60)
                                                         GTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCG                                                                                           >  SRR19973066.2006433/1‑150 (MQ=60)
                                                           ACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTT                                                                                         <  SRR19973066.2319305/150‑1 (MQ=60)
                                                           ACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTT                                                                                         <  SRR19973066.129721/150‑1 (MQ=60)
                                                           ACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTT                                                                                         <  SRR19973066.1943662/150‑1 (MQ=60)
                                                            CCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTA                                                                                        <  SRR19973066.1121022/150‑1 (MQ=60)
                                                            CCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTA                                                                                        <  SRR19973066.1121024/150‑1 (MQ=60)
                                                             CAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAA                                                                                       >  SRR19973066.1478040/1‑150 (MQ=60)
                                                              AGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAG                                                                                      <  SRR19973066.127721/150‑1 (MQ=60)
                                                                TCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCA                                                                                    >  SRR19973066.787119/1‑150 (MQ=60)
                                                                TCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCA                                                                                    >  SRR19973066.786354/1‑150 (MQ=60)
                                                                  CGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGA                                                                                  >  SRR19973066.1048905/1‑150 (MQ=60)
                                                                  CGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGA                                                                                  >  SRR19973066.1338642/1‑150 (MQ=60)
                                                                   GCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAT                                                                                 <  SRR19973066.2374082/150‑1 (MQ=60)
                                                                   GCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAT                                                                                 <  SRR19973066.2374125/150‑1 (MQ=60)
                                                                   GCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGAT                                                                                 <  SRR19973066.570220/150‑1 (MQ=60)
                                                                     AAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTG                                                                               <  SRR19973066.2672449/150‑1 (MQ=60)
                                                                     AAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTG                                                                               >  SRR19973066.2925482/1‑150 (MQ=60)
                                                                      AAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGA                                                                              >  SRR19973066.524144/1‑150 (MQ=60)
                                                                           CCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAG                                                                         >  SRR19973066.2994597/1‑150 (MQ=60)
                                                                           CCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAG                                                                         >  SRR19973066.843855/1‑150 (MQ=60)
                                                                            CCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGC                                                                        >  SRR19973066.1646498/1‑150 (MQ=60)
                                                                            CCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGC                                                                        >  SRR19973066.1648469/1‑150 (MQ=60)
                                                                              TGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAA                                                                      >  SRR19973066.2871227/1‑150 (MQ=60)
                                                                               GAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAA                                                                     <  SRR19973066.1556687/150‑1 (MQ=60)
                                                                                AATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAG                                                                    <  SRR19973066.1188290/150‑1 (MQ=60)
                                                                                AATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAG                                                                    <  SRR19973066.1188334/150‑1 (MQ=60)
                                                                                 ATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGC                                                                   >  SRR19973066.3083286/1‑150 (MQ=60)
                                                                                   ATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGAAAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCG                                                                 <  SRR19973066.3491146/150‑1 (MQ=60)
                                                                                   ATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGAAAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCG                                                                 <  SRR19973066.3491543/150‑1 (MQ=60)
                                                                                      AAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAA                                                              >  SRR19973066.2968890/1‑150 (MQ=60)
                                                                                       AGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAG                                                             <  SRR19973066.1318156/150‑1 (MQ=60)
                                                                                          ATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAA                                                          >  SRR19973066.666921/1‑150 (MQ=60)
                                                                                           TTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAA                                                         <  SRR19973066.1670377/150‑1 (MQ=60)
                                                                                           TTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAA                                                         <  SRR19973066.418631/150‑1 (MQ=60)
                                                                                             CCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAAC                                                       <  SRR19973066.2081092/150‑1 (MQ=60)
                                                                                              CGTAGATGTACAGTTCGTCATGGTTCGCTACAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGCTTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACC                                                      <  SRR19973066.2987128/150‑1 (MQ=60)
                                                                                                  GATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAC                                                  <  SRR19973066.3036448/150‑1 (MQ=60)
                                                                                                  GATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAC                                                  >  SRR19973066.1176777/1‑150 (MQ=60)
                                                                                                  GATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATAC                                                  <  SRR19973066.1499914/150‑1 (MQ=60)
                                                                                                      TACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCC                                              >  SRR19973066.342311/1‑150 (MQ=60)
                                                                                                       ACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCT                                             >  SRR19973066.1143517/1‑150 (MQ=60)
                                                                                                          GTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCT                                          <  SRR19973066.305183/150‑1 (MQ=60)
                                                                                                            TCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCG                                        <  SRR19973066.1724001/150‑1 (MQ=60)
                                                                                                            TCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCG                                        <  SRR19973066.295520/150‑1 (MQ=60)
                                                                                                             CGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGA                                       >  SRR19973066.937027/1‑150 (MQ=60)
                                                                                                              GTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGAT                                      >  SRR19973066.3433547/1‑150 (MQ=60)
                                                                                                                CATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGG                                    <  SRR19973066.515859/150‑1 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                   >  SRR19973066.2992767/1‑150 (MQ=60)
                                                                                                                 ATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGT                                   <  SRR19973066.1095017/150‑1 (MQ=60)
                                                                                                                  TGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTA                                  <  SRR19973066.3440395/150‑1 (MQ=60)
                                                                                                                  TGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTA                                  <  SRR19973066.3440538/150‑1 (MQ=60)
                                                                                                                   GGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAA                                 <  SRR19973066.313142/150‑1 (MQ=60)
                                                                                                                   GGTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAA                                 <  SRR19973066.2025900/150‑1 (MQ=60)
                                                                                                                    GTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAAT                                >  SRR19973066.1099857/1‑150 (MQ=60)
                                                                                                                    GTTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAAT                                >  SRR19973066.2403784/1‑150 (MQ=60)
                                                                                                                     TTCGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATG                               >  SRR19973066.863690/1‑150 (MQ=60)
                                                                                                                       CGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGC                             <  SRR19973066.2610446/150‑1 (MQ=60)
                                                                                                                       CGCTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGC                             <  SRR19973066.2405827/150‑1 (MQ=60)
                                                                                                                         CTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCT                           >  SRR19973066.1937627/1‑150 (MQ=60)
                                                                                                                         CTTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCT                           >  SRR19973066.569239/1‑150 (MQ=60)
                                                                                                                          TTCAGATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTG                          <  SRR19973066.1157998/150‑1 (MQ=60)
                                                                                                                              GATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACC                      >  SRR19973066.115741/1‑150 (MQ=60)
                                                                                                                              GATCGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACC                      >  SRR19973066.115762/1‑150 (MQ=60)
                                                                                                                                 CGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTT                   <  SRR19973066.2672538/150‑1 (MQ=60)
                                                                                                                                 CGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTT                   >  SRR19973066.1352277/1‑150 (MQ=60)
                                                                                                                                 CGTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTT                   <  SRR19973066.2703169/150‑1 (MQ=60)
                                                                                                                                  GTTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTA                  >  SRR19973066.669161/1‑150 (MQ=60)
                                                                                                                                  GTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTA                  >  SRR19973066.1243770/1‑150 (MQ=60)
                                                                                                                                   TTGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTAT                 <  SRR19973066.2958587/150‑1 (MQ=60)
                                                                                                                                    TGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATG                <  SRR19973066.412279/150‑1 (MQ=60)
                                                                                                                                    TGACAGCCGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGNTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATG                >  SRR19973066.1821375/1‑150 (MQ=60)
                                                                                                                                           CGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGT         >  SRR19973066.1980160/1‑150 (MQ=60)
                                                                                                                                           CGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGT         >  SRR19973066.3473613/1‑150 (MQ=60)
                                                                                                                                           CGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGT         >  SRR19973066.3472966/1‑150 (MQ=60)
                                                                                                                                           CGCACTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGT         >  SRR19973066.942217/1‑150 (MQ=60)
                                                                                                                                               CTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCA     <  SRR19973066.1116592/150‑1 (MQ=60)
                                                                                                                                               CTCCATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCA     >  SRR19973066.1631622/1‑150 (MQ=60)
                                                                                                                                                 CCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGG   <  SRR19973066.1185620/150‑1 (MQ=60)
                                                                                                                                                  CATTACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG  <  SRR19973066.737972/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GGCTAATTGCAGGTTCGATTCCTGCAGGGGACACCATTTATCAGTTCGCTCCCATCCGTACCAGTCCGCAAAATCCCCTGAATATCAAGCATTCCGTAGATTTACAGTTCGTCATGGTTCGCTTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTTTACCCACCGGATTTTTACCCATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTTCTCGATGGTAATGGCCTGTACCTTTATGTCCCTGTGTCAGGG  >  CP009273/2459827‑2460122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑