Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,232,997 T→A 56.8% intergenic (+93/‑306) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,232,9970TA56.8% 67.3 / 170.6 127intergenic (+93/‑306)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (41/13);  new base A (34/38);  total (76/51)
Fisher's exact test for biased strand distribution p-value = 1.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 134 total aligned reads displayed.

TTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAA  >  CP009273/3232851‑3233144
                                                                                                                                                    |                                                                                                                                                   
ttttttATCAACGCGTGGTTTAATTATCGGCATGATAAGCAGGGGGGTGTATATTTTTTAATCTGCCTAAGCGGTGTACCCTGAAATTAAATTGCGCACCTTTT‑T‑GTCCCTCTCCCTCCCAGGTTGAGGGTCGGGTTGTGGGTAAttt                                                                                                                                                    <  2:849652/148‑1 (MQ=255)
tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttt                                                                                                                                                    >  1:2571804/1‑148 (MQ=255)
  tattatCACCGCCTGGGTGAATTATCGGCATTATAAGCTGGGGGGTGTATAATTTTTAATCTGCCTAAGCCGTTTACCCTGTAATTAACATGAGCACCTTTT‑T‑CTCCCTCACCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAAtattt                                                                                                                                                  <  2:1058507/148‑1 (MQ=255)
  tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtattt                                                                                                                                                  >  2:1667789/1‑148 (MQ=255)
  tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtattt                                                                                                                                                  >  2:1671517/1‑148 (MQ=255)
     tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGCGGGTCCTTTGTCGc                                                                                                                                               >  2:2206664/1‑148 (MQ=255)
     tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATGTTTCGc                                                                                                                                               >  1:3409562/1‑148 (MQ=255)
        cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                                                                                                                            >  1:2216371/1‑148 (MQ=255)
           cGCCTGGGTGATTTATCGGCATGATAAGCAGGGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGTGGGTGGGGGTGAGGGTAATATTTCGCACCGAt                                                                                                                                         <  2:1083048/148‑1 (MQ=255)
           cGCCTGGGTGAATTATCGGCATGATACGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACTTGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGAt                                                                                                                                         <  2:1083005/148‑1 (MQ=255)
           cGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGAt                                                                                                                                         >  2:2083321/1‑148 (MQ=255)
            tccTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATg                                                                                                                                        >  1:46400/2‑148 (MQ=255)
                gggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCATCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTgg                                                                                                                                    <  1:781616/148‑1 (MQ=255)
                  ttGAATTAGCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTACCATGAGCACCGTTT‑T‑CTCCCACTCCCTTCCAGGGAGTGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGcc                                                                                                                                  <  2:263421/147‑1 (MQ=255)
                  gggAATTATCGGCAAGATAAGCACCGGGGTGGATAATATTTAAACTCCCTAACCCGTGAACCCTCACATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGAGAGGGTAATTATTCGCACCGAAGCTGGcc                                                                                                                                  >  2:1782803/3‑148 (MQ=255)
                  gTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                                                                                                                  >  1:258910/1‑148 (MQ=255)
                   tGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                                                 >  1:2900872/1‑148 (MQ=255)
                    gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                                                >  1:1069/1‑148 (MQ=255)
                    gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTg                                                                                                                                >  1:2519791/1‑148 (MQ=255)
                    gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTg                                                                                                                                >  1:736206/1‑148 (MQ=255)
                         aTCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTacc                                                                                                                           >  1:71852/1‑148 (MQ=255)
                         aTCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTccc                                                                                                                           >  1:1330073/1‑148 (MQ=255)
                               aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CACCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                                     <  2:2243806/148‑1 (MQ=255)
                                      gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                                              >  2:2898440/1‑148 (MQ=255)
                                       ctgggggtTGAATATTTTTAAAACTCCCTACGCGGTGAACCCTGTAATAAAAATCCCCACCTTTA‑C‑CACCCACCCCCTCCCCGGGTGTGGGTGGGGGTGCGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCct                                                                                                             <  1:3066097/144‑1 (MQ=255)
                                             ggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCACTaccc                                                                                                       >  2:2430501/1‑148 (MQ=255)
                                                  aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                  >  2:2406163/1‑148 (MQ=255)
                                                    aaTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                                                >  2:268669/1‑148 (MQ=255)
                                                      tttttAATCTGCCTAAGCCGTGTCCCCTGTCATTAACATGACCACCGTTT‑T‑CTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                                              <  2:3473101/148‑1 (MQ=255)
                                                        tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                                            <  2:3120642/148‑1 (MQ=255)
                                                         ttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                                           >  2:508738/1‑148 (MQ=255)
                                                             tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                                                       >  1:3540311/1‑148 (MQ=255)
                                                             tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                                                       >  1:3540308/1‑148 (MQ=255)
                                                              cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGGGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTACCCGCCCCCTAACCCTCTCCCCAAACCGGGCGAGGGGAc                                                                                      >  1:783602/1‑148 (MQ=255)
                                                                 ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCGGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                                                   >  2:1517511/1‑148 (MQ=255)
                                                                   tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCACTCCCCCAACGGGGCGCGGGGCCTGAcc                                                                                 >  1:2417267/1‑148 (MQ=255)
                                                                   tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGAcc                                                                                 >  1:3379277/1‑148 (MQ=255)
                                                                     cgCCGTGTACCCTGAAATTAACATGCGCCCCGTTT‑G‑CGCCCTCTCCCTGCCCGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                               <  2:183015/147‑1 (MQ=21)
                                                                     aGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                               >  2:1257156/1‑148 (MQ=255)
                                                                     aGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGACGGACCGa                                                                               >  1:179423/1‑148 (MQ=255)
                                                                        cGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTc                                                                            <  2:1064085/148‑1 (MQ=255)
                                                                         gtgtACCCTTTCATAAACATGCGC‑CCGTTT‑TCCCCCCCCCCCCTCCCGGGGTGCGGGGGGGGGTTTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTcc                                                                           <  1:897685/148‑1 (MQ=16)
                                                                            tACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                                                        >  2:2892499/1‑148 (MQ=255)
                                                                              cccTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtgttt                                                                      >  1:1908433/1‑148 (MQ=255)
                                                                              cccTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                                                      >  2:3140170/1‑148 (MQ=255)
                                                                                   tCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                 >  2:3403810/1‑148 (MQ=255)
                                                                                      ttAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                                              >  2:1314688/1‑148 (MQ=255)
                                                                                       tAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGAtgttgt                                                             >  1:2354391/1‑148 (MQ=255)
                                                                                       tAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                                             >  1:571032/1‑148 (MQ=255)
                                                                                         aCATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAAACCACACCCCAAACGGGGCGAGGGGCCTGACCGAGTGCTTTTTTTTtgttttgt                                                           >  1:1027166/1‑144 (MQ=255)
                                                                                          cATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                                          <  2:1019051/148‑1 (MQ=255)
                                                                                          cATGACCACCGTTT‑A‑CGCCCCCGCCCTGCCAGGGAGTGGGTCGGGGTGCGGGCAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                                          <  1:203546/148‑1 (MQ=25)
                                                                                            tGAGCACAGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGTGTGAGGGAACTATTTCGCACCGATGCTGGCCTGTTAACCTCACCCTAACCCTCACCCCAAAAGGGGCGCGGGGACTGACCGAGTCCTGTTTTGTTGTTGtcataa                                                        >  2:2774212/1‑146 (MQ=255)
                                                                                               gCACCGTTT‑T‑CGCCCTCGCCCGGCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                                                     <  1:2229122/148‑1 (MQ=25)
                                                                                                cACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTGTCATCAGTcg                                                    >  1:1184653/1‑147 (MQ=255)
                                                                                                  ccGTTTGT‑CACCGTCCCCCTGCCTGGGTGTGGGTGGGGGTGCGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                                                   <  2:2248311/148‑1 (MQ=25)
                                                                                                  ccGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTGCCCACACCCAAAACCACTCCCCCAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGATGTTTTGATCAGTCTgg                                                  >  1:2790123/1‑148 (MQ=255)
                                                                                                     ttt‑t‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGGTGCCCGCACCCAAAACCACTACCCAAAACGGGCGTGGGGTCGGGCCGAGTCCTGTTTTGTTTGTGTCATCAATGGGGAAg                                               >  1:2692059/1‑148 (MQ=255)
                                                                                                      tt‑t‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTGTGGGAGc                                              >  2:938160/1‑148 (MQ=25)
                                                                                                            tccctcgccctGCCGGGGTGTGGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa                                          <  2:1520873/148‑1 (MQ=25)
                                                                                                               ctctccctTCCCGGGAGAGGGTCGGGGTGAGGGTAATATGTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                                       <  2:503459/148‑1 (MQ=255)
                                                                                                               ctcaccctGCCCGGGAGAGGGTGGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                                       <  2:2684676/148‑1 (MQ=37)
                                                                                                                   ccctTCCAGGGTGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc                                   <  1:2675103/148‑1 (MQ=255)
                                                                                                                    cctTCCTGGGAGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt                                  <  2:585450/148‑1 (MQ=255)
                                                                                                                    cctTCCCGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt                                  <  1:403392/148‑1 (MQ=255)
                                                                                                                    cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt                                  >  1:2628982/1‑148 (MQ=255)
                                                                                                                    cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt                                  <  1:591699/148‑1 (MQ=255)
                                                                                                                    ccgtCCAGGGAGAGGGTCGGGGTGAGGGTAATATGTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt                                  <  2:2600726/145‑1 (MQ=255)
                                                                                                                     ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGCAGCCGCACGTTGGCtt                                 >  2:18664/1‑148 (MQ=255)
                                                                                                                      ttCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt                                <  2:404933/148‑1 (MQ=255)
                                                                                                                          aGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACGGACCGCGTCCTTTTTTGATGTTGGGATCAGTCTGGAAGCCGCACGGTGGCTTTAttt                            >  1:2909902/1‑148 (MQ=37)
                                                                                                                           gggAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtttt                           >  2:3447617/1‑148 (MQ=255)
                                                                                                                           gggAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCGCCCCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGCGTCCGTTTTTTTTGTTGTGATCATTCTGGAAGCCGCACGGTGGCtttttttt                           >  1:441336/1‑148 (MQ=255)
                                                                                                                            ggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttttt                          >  2:2835991/1‑148 (MQ=255)
                                                                                                                             gtttgggTGGGGGGGCTGGTAATATTTCGCACCGATGCGGGCCTGTTCCCCCCCCCCTAACCCTCCCCCCAAACGGGGCGAGGGGTCTGCCCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         <  1:2083321/144‑1 (MQ=255)
                                                                                                                             gagaGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         >  2:3110457/1‑148 (MQ=255)
                                                                                                                             gagaGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         >  2:3348127/1‑148 (MQ=255)
                                                                                                                             gagaGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         <  2:213166/148‑1 (MQ=255)
                                                                                                                             gagaGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         <  2:637847/148‑1 (MQ=255)
                                                                                                                             gagaGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                         <  2:212443/148‑1 (MQ=255)
                                                                                                                              agagGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTCTGGAAGCCGCCCGGTGGGGTTATTTTTAt                        >  1:1900087/1‑148 (MQ=37)
                                                                                                                               gagGGTGGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGACGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATg                       <  1:1548782/148‑1 (MQ=255)
                                                                                                                                agGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATTTTTTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTTTGt                      >  1:2280727/1‑148 (MQ=37)
                                                                                                                                   gTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCaa                   <  2:1530153/148‑1 (MQ=255)
                                                                                                                                   gTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCaa                   <  2:1330073/148‑1 (MQ=255)
                                                                                                                                       gggTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaa               <  2:3309440/148‑1 (MQ=255)
                                                                                                                                        ggTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaaa              <  1:3403810/148‑1 (MQ=255)
                                                                                                                                         gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTTTTGTGATCAGTCTGGAAGCCGCACGGTGGGTTTATTTTTATTTGaaataaat             >  1:2605086/1‑148 (MQ=37)
                                                                                                                                           gaggcaaaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGt           <  1:1648148/142‑1 (MQ=255)
                                                                                                                                           gAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATTTCAAATAAAAGt           >  2:3042955/1‑148 (MQ=255)
                                                                                                                                           gAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACACCCCCAACCGGGCGAGGGGACTGACCGAGTCCGTTTTTTATTTTTTAATAACTCTTGTAGCCCCACCGTGGCGTTATTTTTTTTTAAAATaaaatt           >  1:1391830/1‑145 (MQ=255)
                                                                                                                                           gAGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGt           <  1:1648199/148‑1 (MQ=255)
                                                                                                                                             ggttAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  2:736206/145‑1 (MQ=255)
                                                                                                                                             gggTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  1:3012310/148‑1 (MQ=255)
                                                                                                                                             gggTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  2:2519791/148‑1 (MQ=255)
                                                                                                                                             gggAAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  1:2106871/148‑1 (MQ=255)
                                                                                                                                              gcacaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAc        <  1:3025076/144‑1 (MQ=255)
                                                                                                                                                tAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGATGTTTTCATCAGTCTGGAAGCCGCACGGTTGCGTTATTTTTATGTGAAAGAAATTTAACCa      >  2:1519278/1‑148 (MQ=38)
                                                                                                                                                tAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAccc      >  2:3485255/1‑147 (MQ=255)
                                                                                                                                                    ttttCGCACCGATGCTGGCCTGTTACCCTCACCCAAACCCACTCCCCAAACGGGGCGAGGGGACAGACCGAGTCCTTTTTTTATTTTTTCATAAGTCTTGAAGCCGCACGTTGGCTTTATTTTTTTGTCAAATAAAATTAAACAATaa  >  1:1441895/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTT‑T‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAA  >  CP009273/3232851‑3233144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A