Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,232,997 T→A 27.6% intergenic (+93/‑306) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,232,9970TA27.6% 218.6 / 110.6 138intergenic (+93/‑306)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (77/23);  new base A (23/15);  total (100/38)
Fisher's exact test for biased strand distribution p-value = 5.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 143 total aligned reads displayed.

TTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTA  >  CP009273/3232852‑3233143
                                                                                                                                                 |                                                                                                                                                  
ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                                  >  2:2304958/1‑148 (MQ=255)
       cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                                                                                                                           >  1:2748386/1‑148 (MQ=255)
       cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCAcc                                                                                                                                           >  2:129784/1‑148 (MQ=255)
         aCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                                                                                                                         >  1:1836377/1‑148 (MQ=255)
              tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTg                                                                                                                                    >  2:2769407/1‑148 (MQ=255)
                   gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAGCGATGCTGGCCTg                                                                                                                               >  1:320659/1‑148 (MQ=255)
                   gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                                               >  1:921914/1‑148 (MQ=255)
                   gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                                               >  1:320962/1‑148 (MQ=255)
                      ttATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                                            >  1:3064497/1‑148 (MQ=255)
                       tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTcc                                                                                                                           >  1:2557261/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                         >  1:1155564/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                         >  1:1156840/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                         >  1:1155516/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                         >  2:2897460/1‑148 (MQ=255)
                                 aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcccca                                                                                                                 >  1:2599785/1‑146 (MQ=255)
                                  tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                                                >  2:2139572/1‑148 (MQ=255)
                                    cgccggggggTGTATATTTTTTAATCGGCCTACGCCGTGTCCCGTGTATTAAACTTCACAACGGTTTACACCCTCTCCCTCCGTGGGTGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                                              <  2:787590/142‑1 (MQ=255)
                                    agcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGCACCGATGCTGGCCTGTTCACCTCACCCaaaac                                                                                                              >  1:2998818/1‑146 (MQ=255)
                                     gcgggggggTGTATAATTTTTAAGCGGCCTAGGCGTTTTACCCTTTAATTAACTTGCGCACGTTTTCCCCCCGCTCCCGCCCGGGGTGTGGGGGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                                             <  2:3075126/143‑1 (MQ=16)
                                         cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                         >  2:3351995/1‑148 (MQ=255)
                                              tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCaa                                                                                                    >  1:2820525/1‑148 (MQ=255)
                                                gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTATCCCCaaaa                                                                                                  >  2:1906215/1‑147 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                 >  2:2969892/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                 >  1:2370422/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                 >  2:1765905/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                 >  2:1765708/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGGGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                 >  1:2096926/1‑148 (MQ=255)
                                                    aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCACTCCCCAAAAgggg                                                                                              >  1:57114/1‑148 (MQ=255)
                                                    aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTACCCTCAACCAAACCCTAACCCCAAAAgggg                                                                                              >  2:2718200/1‑148 (MQ=255)
                                                     tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                                             >  2:2818344/1‑148 (MQ=255)
                                                     tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                                             >  2:3001049/1‑148 (MQ=255)
                                                      ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCg                                                                                            >  1:2969446/1‑148 (MQ=255)
                                                         taagcgcccTATGCGGTGTACCCTGTCTTTAACAGGAGCACGGTTTTCGCCCTCCCCCTACCCGGGAGTGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                                         <  1:2889648/141‑1 (MQ=21)
                                                          aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCGAggg                                                                                        >  1:544348/1‑148 (MQ=255)
                                                          aaTCGGCCTAAGCGGTGTCCCCTTAATTTACCTTGCGCCCGTTTTTCTCCCTCGCCCGCCCGGGGTGTGGGGGGGGGTTGGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                                                        <  2:816649/148‑1 (MQ=12)
                                                             cTGCCTAAGCCGTGTACCCTGTCCTTAACATGAGCACCGTTTTCTCCCTCTCCCTGCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                                                     >  2:268845/1‑148 (MQ=255)
                                                             cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                                                     <  2:2326907/148‑1 (MQ=255)
                                                             cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                                                     <  2:532449/148‑1 (MQ=255)
                                                               gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGGTGCCCCCACCCCAACCCACACCCCCAACCGGGCGCGGGGACTg                                                                                   >  1:609732/1‑148 (MQ=255)
                                                                ccTACGCCGTGTACCCTGACATTAACTGGCGCCCCGTTTTCTCCCTCTCCCTGCCCGGGAGTGGGTGGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                                                  <  2:1224428/148‑1 (MQ=21)
                                                                   aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTACCCTCACCCAAAACCCCTCCCCAAAAAGCGCGAGGGGAcataacc                                                                               >  1:2780838/1‑142 (MQ=255)
                                                                    aGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                              >  2:613392/1‑148 (MQ=255)
                                                                     gCCGTGTACCCTGTCATTAACCTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                                             <  2:1050306/148‑1 (MQ=255)
                                                                     gCCGTGTACCCTGTCATTAACATGAGCACCGTTTGCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                                             <  2:1050762/148‑1 (MQ=255)
                                                                       cGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGCCTGACCGCGTc                                                                           >  1:3104033/1‑148 (MQ=255)
                                                                          gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtt                                                                        <  2:2852768/148‑1 (MQ=255)
                                                                           tACCGTGTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                                                       <  2:598480/148‑1 (MQ=25)
                                                                             cccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCTAAACCTCACCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                                                     >  1:1304382/1‑148 (MQ=255)
                                                                              ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAAGGGGCGAGGGGACTGACCGAGTCCtttttt                                                                    >  2:2859076/1‑148 (MQ=255)
                                                                               cagTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTg                                                                   >  1:261975/3‑148 (MQ=255)
                                                                                 gtaaTTAAAATGCGCACCGTTTTCCCCCTCCCCCTCCCTGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                                                 <  2:577284/145‑1 (MQ=21)
                                                                                 gTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                                                 <  2:2367683/148‑1 (MQ=255)
                                                                                 gTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAAAGGGGCGAGGGGACTGACCGAGTCCTTTTTTgtt                                                                 >  1:3375528/1‑146 (MQ=255)
                                                                                  tCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTttgttg                                                                >  2:2900182/1‑148 (MQ=255)
                                                                                   cATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTtgttgt                                                               >  1:1674299/1‑148 (MQ=255)
                                                                                    aTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                                              >  1:3184617/1‑148 (MQ=255)
                                                                                            gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                                                      >  2:491530/1‑148 (MQ=255)
                                                                                            gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCAAACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATAAg                                                      >  2:495681/1‑148 (MQ=255)
                                                                                              gCACGGTTTTCTCCCGCGCCCTCCCCGGTAGAGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                                                    <  2:1836377/148‑1 (MQ=25)
                                                                                              gCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTAAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                                                    <  1:2932861/148‑1 (MQ=255)
                                                                                                 ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAACGGGGCGAGGGGACTGTCCGAGTCCTTTTTTGATGTTGTCATAAGTCTgg                                                 >  1:3212284/1‑148 (MQ=255)
                                                                                                    ttttCCCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                                              <  1:135905/148‑1 (MQ=255)
                                                                                                     tttCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACACCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTGATTTTGTCATCAGTCTGGAAGc                                             >  1:2522951/1‑148 (MQ=255)
                                                                                                     tttCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCGCACCCAAACCCACTCCCCAAACGGGGGGAGGGGACTGACCGAGTCCTGTTTTTATGTTTTGATAAGTCTGGTAGc                                             >  1:2522920/1‑148 (MQ=255)
                                                                                                       tgtccctatccctTCCTGGGAGTGGGTGGGGGTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                                           <  2:2665708/146‑1 (MQ=37)
                                                                                                       tctccctctcccTTCCAGGTTGTGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                                           <  2:3212309/148‑1 (MQ=37)
                                                                                                       tctccctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATTTTTTCATCAGTCTGGAAGCCg                                           >  1:2029624/1‑148 (MQ=255)
                                                                                                        ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACCGGGCGAGGGGACTGACCGAGTCCTGTTTTGTTGTTGTCATCAGTCGGGAAGCCGc                                          >  1:2852005/1‑148 (MQ=25)
                                                                                                         tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa                                         >  2:1955994/1‑148 (MQ=255)
                                                                                                           cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg                                       >  2:3451652/1‑148 (MQ=255)
                                                                                                            ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGGAGCCGCACGt                                      >  2:2918221/1‑148 (MQ=255)
                                                                                                               tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGATGTTGGCATCAGTCTGGACGCCGCACGTTgg                                   >  2:1934826/1‑148 (MQ=255)
                                                                                                               cccctCCCGGGGAGTGGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg                                   <  2:3499754/147‑1 (MQ=37)
                                                                                                               cccctCCCCGGGAGTGGGGGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg                                   <  2:2145876/147‑1 (MQ=37)
                                                                                                                     cctgggAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                             <  2:184367/145‑1 (MQ=255)
                                                                                                                     ccAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                             <  2:3149182/148‑1 (MQ=255)
                                                                                                                       aGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                           >  2:2177716/1‑148 (MQ=255)
                                                                                                                         ggAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAACCCACAACCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGTTTTTTTTTTTCATAAGTCGGGAAACCCCCCCTTGGCTTTAttttt                         >  1:784902/1‑148 (MQ=255)
                                                                                                                           agagGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTAt                       >  2:1848827/1‑148 (MQ=255)
                                                                                                                           agagGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTAt                       >  2:2012948/1‑148 (MQ=255)
                                                                                                                            gcgggTCGGGGTGAGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATg                      <  2:3341212/146‑1 (MQ=255)
                                                                                                                             agGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAAACCACACCCCAAAAGGGGCGGGGGGACTGTCCGAGTCCGTTTTTTTTGTTTTGAAAAGTCTGGGAGCCCCCCCGTGGGTtttttttttttt                     >  1:1661019/1‑144 (MQ=255)
                                                                                                                             agGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCAAAACCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTCTGGAAGCCGCCCGATGGCGTTAttttttttt                     >  1:1968555/1‑144 (MQ=255)
                                                                                                                              gggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTTTCATCAGTCTGGAAGCCGCACGTTGGCGTTATTTTTTTGTc                    >  1:2364948/1‑148 (MQ=37)
                                                                                                                                gTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAACGGGGCGCGGGGACTGACCGCGTCCTTTTTTTATGTTTTCATCAGTCTGGTATCCCCACGGTGGGTTTTTTTTTTTGTGaa                  >  1:1919980/1‑148 (MQ=25)
                                                                                                                                gTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATAAGTCTGGGAGCCGCACGTTGGGTTTATTTTTAtttgaa                  >  1:449784/1‑143 (MQ=255)
                                                                                                                                    ggtttaGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGTGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaa              <  1:49699/143‑1 (MQ=255)
                                                                                                                                      gggggggAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAAt            <  2:1156840/144‑1 (MQ=255)
                                                                                                                                      gTGAGGCAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAAt            <  2:2748386/148‑1 (MQ=255)
                                                                                                                                       tgtgggTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATg           <  1:601309/145‑1 (MQ=255)
                                                                                                                                       tGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATg           <  2:1872946/148‑1 (MQ=255)
                                                                                                                                       tGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATg           <  2:719978/148‑1 (MQ=255)
                                                                                                                                        gAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGt          <  2:3184067/148‑1 (MQ=255)
                                                                                                                                          gggAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa        <  2:2561687/148‑1 (MQ=255)
                                                                                                                                             tAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAATAAATTTAAACa     >  2:2019014/1‑148 (MQ=255)
                                                                                                                                             tAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCa     >  2:217341/1‑148 (MQ=255)
                                                                                                                                             tAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCa     >  2:2021209/1‑148 (MQ=255)
                                                                                                                                              caTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCAt    <  2:2491572/147‑1 (MQ=255)
                                                                                                                                                tttttCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGTATTCCGCACTTTGGCTTTATTTTTATGTCAACGAAATGTAAACATTa  <  2:2969454/148‑1 (MQ=38)
                                                                                                                                                tttttCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTa  <  2:2737923/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTA  >  CP009273/3232852‑3233143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A