Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,232,997 T→A 18.8% intergenic (+93/‑306) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,232,9970TA18.8% 276.9 / 59.4 117intergenic (+93/‑306)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (73/22);  new base A (11/11);  total (84/33)
Fisher's exact test for biased strand distribution p-value = 1.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 121 total aligned reads displayed.

TTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAA  >  CP009273/3232852‑3233144
                                                                                                                                                   |                                                                                                                                                   
ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtatt                                                                                                                                                   >  1:3253564/1‑148 (MQ=255)
 tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                                                  >  2:2728805/1‑148 (MQ=255)
  attatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTCATTTTTc                                                                                                                                                 >  2:1358384/1‑148 (MQ=255)
   ttatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                                                >  1:1078421/1‑148 (MQ=255)
     atCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCa                                                                                                                                              >  2:1475247/1‑148 (MQ=255)
     atCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCAGTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCa                                                                                                                                              >  2:1475233/1‑148 (MQ=255)
        aaCGCGTGGGTTAATTATCGGCATGATAAGCAGCGGGGTGGATATTTTTTAATCTGCATAAGCCGTGTACCCTGTATTTAA‑CATGAGCACCGTTTT‑CCCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATATTTCGCACCg                                                                                                                                           <  2:3285100/148‑1 (MQ=255)
         aCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                                                                                                                          >  1:1785210/1‑148 (MQ=255)
          cGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                                                         >  2:3114898/1‑148 (MQ=255)
          cGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                                                         >  1:964294/1‑148 (MQ=255)
            ccTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGc                                                                                                                                       >  2:3451766/1‑148 (MQ=255)
              tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTg                                                                                                                                     >  1:730544/1‑148 (MQ=255)
              tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTg                                                                                                                                     >  1:1543748/1‑148 (MQ=255)
              tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTg                                                                                                                                     >  2:205080/1‑148 (MQ=255)
                gttGATTTATGGGCAGGATACGCAGGGGGGTGGATATTTTTTATTCTGCCTCCGCCGTGTACCCTGTAATTAA‑CATGCGCACCGTTTT‑CGCCCTCTCCCTCCCCGGGAGCGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGc                                                                                                                                   <  2:3492358/146‑1 (MQ=255)
                 gTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcg                                                                                                                                  >  2:330135/1‑147 (MQ=255)
                   gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                                                >  1:270040/1‑148 (MQ=255)
                       tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                                                                                                            >  1:3103797/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                          >  2:2680028/1‑148 (MQ=255)
                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                          >  2:2680091/1‑148 (MQ=255)
                          cGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                                                         >  2:2169257/1‑148 (MQ=255)
                          cGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                                                         >  2:3479223/1‑148 (MQ=255)
                           ggctttaTACGCCGCGGGTTGTATTTTTTTTATTCTGCCTACGCGGTGTCCCCTTAATTTAA‑CTTGCGCCCGTTTTT‑CTCCCTCTCCCTTCCTGGTTGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTc                                                                                                                        <  2:954007/142‑1 (MQ=255)
                             cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                                                      >  1:3527831/1‑148 (MQ=255)
                             cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCAc                                                                                                                      >  1:3529497/1‑148 (MQ=255)
                             cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                                                      >  1:183330/1‑148 (MQ=255)
                                   aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTACCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTACCCACACCCTAAc                                                                                                                >  2:736953/1‑148 (MQ=255)
                                      cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCct                                                                                                             >  2:1651925/1‑148 (MQ=255)
                                               ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                    >  2:2434379/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                                  >  2:880179/1‑148 (MQ=255)
                                                 aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCGCTCCCCCAAcc                                                                                                  >  1:1093533/1‑147 (MQ=255)
                                                  tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                                                 >  2:859108/1‑148 (MQ=255)
                                                  tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                                                 >  1:525070/1‑148 (MQ=255)
                                                  tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGAACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCACACgg                                                                                                 >  2:858901/1‑148 (MQ=255)
                                                   attttttAATCTGACTACGCGTTGTCCCCTGTAATTAA‑CATGAGCACGTTTTT‑CTCCCTCCCCCTTCCTGGTTGTGGGTGGGGGTGTGGGAAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                                                <  1:1454004/146‑1 (MQ=21)
                                                   aaTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑ATCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                                                >  2:2370713/1‑148 (MQ=255)
                                                      ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCg                                                                                             >  2:3156390/1‑148 (MQ=255)
                                                      ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTACCCAAACGGGGCg                                                                                             >  1:2857492/1‑148 (MQ=255)
                                                        ttAATCTGCCTACGCGGTGTACCCGGACATTAA‑CATGAGCACCGTTTT‑CCCCCTCTCCCTGCCGGGGTGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                                           <  2:2515508/148‑1 (MQ=25)
                                                        ttAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                                           >  2:613620/1‑148 (MQ=255)
                                                         tattCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCATTCCTGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                                          <  2:3284843/145‑1 (MQ=255)
                                                         tAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                                          <  2:3284807/148‑1 (MQ=255)
                                                           aTCTGCCTACGCCGTGCACCCTGT‑ATTAACCTTTCGCACGTTTTT‑CCCCCTCCCCCTTCCCGGTTGTGGGTGGGGGTGAGGGAAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgggg                                                                                        <  1:145110/148‑1 (MQ=12)
                                                           aTCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACACCCCAAACGGGGCGAgggg                                                                                        >  1:3039299/1‑148 (MQ=255)
                                                            tCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                                                       >  1:966087/1‑148 (MQ=255)
                                                                ccTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTGCCCTCACCCTAACCCACAACCCAAACGGGGCGAGGGGACTGa                                                                                   >  1:2464265/1‑148 (MQ=255)
                                                                  tAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                                                 >  2:1287548/1‑148 (MQ=255)
                                                                  tAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCACCGCCACCAAACCCCCACCCCAAACCCGGCGGGGGGCCTGAcc                                                                                 >  1:9818/1‑148 (MQ=255)
                                                                   aaGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                                                >  2:1539344/1‑148 (MQ=255)
                                                                       cGTGTACCCTGACATTAA‑CTGGAGCACGGTTTT‑CCCCCTCCCCCTCCCGGGGTGTGGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTc                                                                            <  2:3141915/148‑1 (MQ=21)
                                                                           tACCCTTTCATTAA‑CTTGACCACGTTTTT‑CCCCCTCGCCCTTCCTGGGAGTGGGTCGGGGTGCGGGTAATATTTCGCACCGATGCTGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                                                        <  1:2315298/148‑1 (MQ=18)
                                                                            aCCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                                                       <  2:183330/148‑1 (MQ=255)
                                                                             cccTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtggtt                                                                      >  1:2819487/1‑144 (MQ=255)
                                                                             cccTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGCGTCCttttt                                                                      >  1:3021199/1‑148 (MQ=255)
                                                                               cTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCGAACCCACTCCCCACACCGGGCGCGGGGCCTGACCGAGTCCttttttt                                                                    >  1:211124/1‑147 (MQ=255)
                                                                               cTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                                                    >  2:2366535/1‑148 (MQ=255)
                                                                                  tCATTAA‑CATTAGCACCTTTTG‑CTCCCTCGCCCTTCCTGGGTGTGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                 <  2:2471394/148‑1 (MQ=25)
                                                                                  tCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                 >  2:2946633/1‑148 (MQ=255)
                                                                                  acATTAA‑CTGGAGC‑CCGTTTTGCTCCCGCTCCGTCCCGGGGTGTGGGGGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                 <  2:2472961/147‑1 (MQ=21)
                                                                                   cATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACAGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAACGAGTCCTTTTTTGAtgt                                                                >  2:1240339/1‑148 (MQ=255)
                                                                                       aa‑CTTGCGCCCCGTTTT‑CCCCCGCCCCCTACCCGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                                            <  2:2076852/148‑1 (MQ=21)
                                                                                          cATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCGCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTAtgttgtgtt                                                          >  1:3302279/1‑145 (MQ=255)
                                                                                          cATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGtcat                                                          >  1:3302313/1‑148 (MQ=255)
                                                                                             gAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                                                       >  2:2708924/1‑148 (MQ=255)
                                                                                                   cGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCTAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                                                 >  1:901366/1‑148 (MQ=255)
                                                                                                     tttt‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCACCCCAAACCGGGCGAGGGGCCTGACCGAGTCCTTTTTTTATGTTGTCATCATTCTGGAAg                                               >  1:2955615/1‑148 (MQ=255)
                                                                                                      tttccgccctccccctccCCGGGAGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                                               <  2:1547450/142‑1 (MQ=25)
                                                                                                      ttt‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                                              >  2:563214/1‑148 (MQ=255)
                                                                                                      ttt‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACTCCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTTTTTTCTTCAGTCTTGGAGc                                              >  1:1057923/1‑148 (MQ=255)
                                                                                                             cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATTTTTTCATCAGTCTGGAAGCCGCACg                                        >  2:1768636/1‑148 (MQ=255)
                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                                       >  1:2501893/1‑148 (MQ=255)
                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCACACCCCCAACGGGGCGAGGGGCCTGACCGAGGCCTTTTTTTATGTTGTGATCAGGGGGGTAGCCGccccg                                       >  1:437026/1‑144 (MQ=255)
                                                                                                                ctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTGCCCGCCCCCGAACCCACACCCCAAACGGGGGGGGGGGGCGGGCCGCGGGCTTTTTTGTTGTTTTGATCAGTATGGGAGCCGccccgtg                                     >  1:655642/1‑142 (MQ=255)
                                                                                                                 tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCGCACCCAAACCCTCTCCCCAAACCGGGCGAGGGGGCTGACCGAGTCCTTTTTTGTTGTTGTGATCAGTCTGGAAGCCGCACGGTgg                                    >  1:1069127/1‑148 (MQ=255)
                                                                                                                    ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTAATCAGTCTGGAAGCCGCACGTTGGCtt                                 >  2:75187/1‑148 (MQ=255)
                                                                                                                      tCCAGTTAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAACGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa                               <  1:1768636/148‑1 (MQ=255)
                                                                                                                      tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTTTTGTGATCAGTCTGGAAGCCGCACGGTGGCTTTa                               >  1:2430373/1‑148 (MQ=255)
                                                                                                                      tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa                               >  2:191880/1‑148 (MQ=255)
                                                                                                                      tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAAACCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATTTTGTGATCAGTCTGGAAGCCGCACGTTGGCTTTa                               >  1:2430316/1‑148 (MQ=255)
                                                                                                                      tCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTACCCGCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTTTTGTGATCCCTATGGAACCCCCACGGTGGGtttt                               >  1:3714367/1‑147 (MQ=21)
                                                                                                                         cgggAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                            <  2:560782/147‑1 (MQ=255)
                                                                                                                         aGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                            >  2:1221326/1‑148 (MQ=255)
                                                                                                                          gggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtttt                           >  2:2852138/1‑148 (MQ=255)
                                                                                                                           ggAGAGGTTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttttt                          <  2:1829736/148‑1 (MQ=255)
                                                                                                                           ggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTGCCCGCACCCAAAACCACACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTTTGTTTTGATCAGTCTGGAAGCCGCACGGTGGCTTTAttttt                          >  1:2682714/1‑148 (MQ=255)
                                                                                                                           ggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACTCCCCAAACGGGGCGAGGGGCCTGGCCGAGTCCTTTTTTTGTGTTGTGATGAGTCTGGGAGCCGCCCGGTGGGGTTAttttt                          >  1:2682697/1‑148 (MQ=255)
                                                                                                                           ggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAACCCACAACCCCAACCGGGCGAGGGGCCGGACCGCGGCCTTTTTTTTTGTTTTGATGAAGCTGGGAGCCCCCCCGTGGGTtttttttt                          >  1:765665/1‑148 (MQ=255)
                                                                                                                              gtgggTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATg                       <  1:2510296/146‑1 (MQ=255)
                                                                                                                              gagGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTACCCAAACGGGGCGAGGGGACAGTCCGAGTCCTGTTTTGTTGGTGTGCTCAGTCTGGGAGCCGCACGTTGGGGTTTTTTTTATg                       >  1:2391822/1‑148 (MQ=255)
                                                                                                                               agGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGt                      >  2:112915/1‑148 (MQ=255)
                                                                                                                                 ggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCa                    >  2:1225242/1‑148 (MQ=255)
                                                                                                                                      gggTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTCTGGAAGCCGCACGTTGGGTTTTTTTTTATTTCAAAGaa               >  1:2755120/1‑148 (MQ=255)
                                                                                                                                       ggTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaaa              <  1:3135741/148‑1 (MQ=255)
                                                                                                                                        gtgtGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAAt             <  2:2612177/148‑1 (MQ=255)
                                                                                                                                         tGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCGTTATTTTTATGTCAAAGAAATg            >  2:3552628/1‑148 (MQ=255)
                                                                                                                                            gggTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  2:506613/148‑1 (MQ=255)
                                                                                                                                            gggTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTaa         <  2:2427195/148‑1 (MQ=255)
                                                                                                                                              cccaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAcc       <  1:3313091/145‑1 (MQ=255)
                                                                                                                                               aaaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCa      <  1:1190423/147‑1 (MQ=255)
                                                                                                                                                   ttttCGCACCGATGCTGGCCTGTTCCCCACACCCAAAACCACAACCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATAAGTCTGGAATCCCCCCCTTGGCTTTATTTTTATTTAAAATAAAAGTAAACATTaa  >  1:2398111/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAA‑CATGAGCACCGTTTT‑CTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAA  >  CP009273/3232852‑3233144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A