Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,217,289 A→T 100% H433L (CAC→CTC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,217,2890AT98.0% 647.2 / 2.1 202H433L (CAC→CTC) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base T (101/97);  total (101/101)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA  >  CP009273/3217144‑3217433
                                                                                                                                                 |                                                                                                                                                
gAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcg                                                                                                                                                >  2:119470/1‑148 (MQ=255)
gAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcg                                                                                                                                                <  1:244845/148‑1 (MQ=255)
gAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcg                                                                                                                                                >  2:3082855/1‑148 (MQ=255)
   cTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCgcgc                                                                                                                                             >  2:3050668/1‑148 (MQ=255)
     gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAg                                                                                                                                           >  2:2516477/1‑148 (MQ=255)
           tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAc                                                                                                                                     >  1:2633060/1‑148 (MQ=255)
           tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAc                                                                                                                                     >  1:1985553/1‑148 (MQ=255)
            atCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAcc                                                                                                                                    <  2:462032/148‑1 (MQ=255)
             tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCa                                                                                                                                   <  2:1781243/148‑1 (MQ=255)
              cTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAt                                                                                                                                  <  1:1890177/148‑1 (MQ=255)
               tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATc                                                                                                                                 <  1:2105178/148‑1 (MQ=255)
                   gCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTc                                                                                                                             >  2:2561679/1‑148 (MQ=255)
                    ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCg                                                                                                                            >  1:3421299/1‑148 (MQ=255)
                    ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCg                                                                                                                            <  2:572635/148‑1 (MQ=255)
                     cTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGa                                                                                                                           >  2:3410273/1‑148 (MQ=255)
                      tGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGat                                                                                                                          <  2:1236659/148‑1 (MQ=255)
                       gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatc                                                                                                                         <  2:223809/148‑1 (MQ=255)
                         ttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcat                                                                                                                       <  2:2325874/148‑1 (MQ=255)
                            tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcat                                                                                                                    <  2:3451628/148‑1 (MQ=255)
                             gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                   <  2:2967435/148‑1 (MQ=255)
                             gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                   >  2:1910595/1‑148 (MQ=255)
                             gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                   >  2:1710273/1‑148 (MQ=255)
                                ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTgg                                                                                                                <  2:1996341/148‑1 (MQ=255)
                                 gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGt                                                                                                               <  2:3557296/148‑1 (MQ=255)
                                 gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGt                                                                                                               <  2:1512402/148‑1 (MQ=255)
                                 gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGt                                                                                                               <  2:2477345/148‑1 (MQ=255)
                                     aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt                                                                                                           <  2:2147849/148‑1 (MQ=255)
                                      aaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTg                                                                                                          >  2:1923493/1‑148 (MQ=255)
                                      aaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTg                                                                                                          <  1:83841/148‑1 (MQ=255)
                                        ccGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTggg                                                                                                        >  1:3202077/1‑148 (MQ=255)
                                         cGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGc                                                                                                       >  2:1616954/1‑148 (MQ=255)
                                          gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCa                                                                                                      <  2:605308/148‑1 (MQ=255)
                                           aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa                                                                                                     >  1:1791509/1‑148 (MQ=255)
                                           aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa                                                                                                     <  1:2368363/148‑1 (MQ=255)
                                            cGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                    <  2:703438/148‑1 (MQ=255)
                                            cGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                    <  1:3261293/148‑1 (MQ=255)
                                              tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGa                                                                                                  >  1:2748045/1‑148 (MQ=255)
                                               cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                 <  1:2347186/148‑1 (MQ=255)
                                               cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                 >  2:3493873/1‑148 (MQ=255)
                                                 aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATc                                                                                               <  1:60351/148‑1 (MQ=255)
                                                 aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATc                                                                                               >  1:1117237/1‑148 (MQ=255)
                                                   tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                             >  2:2913945/1‑148 (MQ=255)
                                                     gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                           <  1:2932893/148‑1 (MQ=255)
                                                        cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGTAAAACCATCCGTCGATCATCCTCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                        >  1:3317384/1‑148 (MQ=255)
                                                        cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                        >  2:2617689/1‑148 (MQ=255)
                                                        cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                        >  1:1549999/1‑148 (MQ=255)
                                                          gCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATgg                                                                                      <  1:3529966/148‑1 (MQ=255)
                                                              tGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                  <  1:2758374/148‑1 (MQ=255)
                                                               gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATAATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTa                                                                                 <  2:3516619/148‑1 (MQ=255)
                                                                 tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                               <  1:1220003/148‑1 (MQ=255)
                                                                 tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                               <  2:3239308/148‑1 (MQ=255)
                                                                 tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                               <  1:1085562/148‑1 (MQ=255)
                                                                  aaTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACa                                                                              <  2:2456659/148‑1 (MQ=255)
                                                                   aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                             <  2:1239927/148‑1 (MQ=255)
                                                                      tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcg                                                                          <  1:257122/148‑1 (MQ=255)
                                                                          cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcgcg                                                                      <  2:901426/148‑1 (MQ=255)
                                                                            atatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAt                                                                    >  1:173279/1‑148 (MQ=255)
                                                                                 aGCCGTATTACCGACTATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCTGCTATGGCTGTAACATCCGCGCGATGTAcc                                                               <  1:902208/148‑1 (MQ=255)
                                                                                 aGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAcc                                                               <  1:1005722/148‑1 (MQ=255)
                                                                                  gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                              >  2:1889447/1‑148 (MQ=255)
                                                                                     gTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                           >  1:688110/1‑148 (MQ=255)
                                                                                      tATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcg                                                          >  2:2636988/1‑148 (MQ=255)
                                                                                       aTTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcgg                                                         >  1:2605738/1‑148 (MQ=255)
                                                                                        ttACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggc                                                        >  1:644718/1‑148 (MQ=255)
                                                                                           ccGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                     <  1:2554435/148‑1 (MQ=255)
                                                                                             gacgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAg                                                   <  1:3050668/148‑1 (MQ=255)
                                                                                               cgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCAGATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGTCTATGGATGTAACATCCGCGCGATGTACCATGCGGCGAAAgcg                                                 <  1:622643/148‑1 (MQ=255)
                                                                                                gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcgc                                                <  2:1550406/148‑1 (MQ=255)
                                                                                                gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAACGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcgc                                                <  1:2315744/148‑1 (MQ=255)
                                                                                                 aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                               <  2:1027079/148‑1 (MQ=255)
                                                                                                  tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTg                                              >  1:722163/1‑148 (MQ=255)
                                                                                                   ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                             <  1:2137700/148‑1 (MQ=255)
                                                                                                      cAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATg                                          >  2:504841/1‑148 (MQ=255)
                                                                                                      cAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATg                                          >  2:1486531/1‑148 (MQ=255)
                                                                                                       aGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGa                                         <  1:2192934/148‑1 (MQ=255)
                                                                                                         tGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGaca                                       >  1:772399/1‑148 (MQ=255)
                                                                                                           ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcg                                     >  2:2483502/1‑148 (MQ=255)
                                                                                                             aaaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                   <  2:1117237/148‑1 (MQ=255)
                                                                                                             aaaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                   >  2:2370558/1‑148 (MQ=255)
                                                                                                                   tCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTCTGGCTGTAACATCCGCGCGAGGTACCATGCGGCGTAAGCGCTGGATGACACGCGACTGGt                             >  2:2389358/1‑148 (MQ=255)
                                                                                                                    cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                            >  1:558837/1‑148 (MQ=255)
                                                                                                                      aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                          <  1:1181155/148‑1 (MQ=255)
                                                                                                                      aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                          <  2:2266239/148‑1 (MQ=255)
                                                                                                                                  ttGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga              <  1:2516477/148‑1 (MQ=255)
                                                                                                                                  ttGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga              <  1:1616954/148‑1 (MQ=255)
                                                                                                                                     ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg           >  2:519800/1‑148 (MQ=255)
                                                                                                                                     ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg           >  1:3491390/1‑148 (MQ=255)
                                                                                                                                     ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg           <  1:481558/148‑1 (MQ=255)
                                                                                                                                     ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg           >  1:1707266/1‑148 (MQ=255)
                                                                                                                                       cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg         >  1:945153/1‑148 (MQ=255)
                                                                                                                                        gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGa        >  1:2024980/1‑148 (MQ=255)
                                                                                                                                        gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGa        <  2:157145/148‑1 (MQ=255)
                                                                                                                                        gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGa        <  2:167687/148‑1 (MQ=255)
                                                                                                                                         ccATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAt       >  1:3002148/1‑148 (MQ=255)
                                                                                                                                          cATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGATCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg      <  1:1710273/148‑1 (MQ=255)
                                                                                                                                           atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc     <  1:3119170/148‑1 (MQ=255)
                                                                                                                                           atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc     <  1:15008/148‑1 (MQ=255)
                                                                                                                                           atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc     <  1:1277303/148‑1 (MQ=255)
                                                                                                                                           atatCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc     <  2:1927378/148‑1 (MQ=255)
                                                                                                                                            tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt    >  1:2869895/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA  >  CP009273/3217144‑3217433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437
                                                                                                                                                     |                                                                                                                                                    
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCT                                                                                                                                                      >  SRR19973066.1149661/1‑150 (MQ=60)
  ACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAACGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCG                                                                                                                                                    >  SRR19973066.3011831/1‑150 (MQ=60)
    GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCG                                                                                                                                                  <  SRR19973066.246750/150‑1 (MQ=60)
         GTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAA                                                                                                                                             >  SRR19973066.2844836/1‑150 (MQ=60)
             GATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAC                                                                                                                                         >  SRR19973066.1999552/1‑150 (MQ=60)
             GATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAC                                                                                                                                         >  SRR19973066.2650793/1‑150 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                    <  SRR19973066.1903654/150‑1 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                    <  SRR19973066.1903642/150‑1 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                    >  SRR19973066.1488141/1‑150 (MQ=60)
                   TACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCG                                                                                                                                   <  SRR19973066.2119686/150‑1 (MQ=60)
                      GGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCG                                                                                                                                >  SRR19973066.3444180/1‑150 (MQ=60)
                       GCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGA                                                                                                                               >  SRR19973066.892791/1‑150 (MQ=60)
                        CCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGAT                                                                                                                              <  SRR19973066.3257616/150‑1 (MQ=60)
                                       GGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATAATCATCTGGTCGCT                                                                                                               >  SRR19973066.3540043/1‑150 (MQ=60)
                                        GAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTG                                                                                                              >  SRR19973066.158591/1‑150 (MQ=60)
                                         AAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGG                                                                                                             >  SRR19973066.99775/1‑150 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                            >  SRR19973066.3223316/1‑150 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                            >  SRR19973066.3223329/1‑150 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                            <  SRR19973066.84778/150‑1 (MQ=60)
                                             CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAA                                                                                                         >  SRR19973066.1804247/1‑150 (MQ=60)
                                             CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAA                                                                                                         >  SRR19973066.1804264/1‑150 (MQ=60)
                                              GACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAT                                                                                                        >  SRR19973066.622771/1‑150 (MQ=60)
                                               ACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG                                                                                                       <  SRR19973066.582332/150‑1 (MQ=60)
                                               ACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG                                                                                                       <  SRR19973066.2384388/150‑1 (MQ=60)
                                               ACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG                                                                                                       <  SRR19973066.896094/150‑1 (MQ=60)
                                                CGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGA                                                                                                      >  SRR19973066.2766535/1‑150 (MQ=60)
                                                CGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGA                                                                                                      <  SRR19973066.3282741/150‑1 (MQ=60)
                                                 GTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAA                                                                                                     >  SRR19973066.2014454/1‑150 (MQ=60)
                                                  TCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAT                                                                                                    <  SRR19973066.2630921/150‑1 (MQ=60)
                                                   CGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATC                                                                                                   <  SRR19973066.2363161/150‑1 (MQ=60)
                                                   CGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATC                                                                                                   <  SRR19973066.2363149/150‑1 (MQ=60)
                                                   CGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATC                                                                                                   >  SRR19973066.1125297/1‑150 (MQ=60)
                                                    GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAGACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCC                                                                                                  >  SRR19973066.2767317/1‑150 (MQ=60)
                                                     AATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCG                                                                                                 <  SRR19973066.61098/150‑1 (MQ=60)
                                                        CGCAGGGCTTTGCTAATGTCGGCGATATTAGCAGTATTACCGACGATCCGCTGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATGTGGTCGCTGGGCAATGAATCCGGCT                                                                                              <  SRR19973066.704696/150‑1 (MQ=60)
                                                         GCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTA                                                                                             <  SRR19973066.2952412/150‑1 (MQ=60)
                                                          CACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGTAAAACCATCCGTCGATCATCCTCTGGTCGCTGGGCAATGAATCCGGCTAT                                                                                            >  SRR19973066.3339408/1‑150 (MQ=60)
                                                          CACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAT                                                                                            >  SRR19973066.1561108/1‑150 (MQ=60)
                                                             GGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGC                                                                                         >  SRR19973066.2176613/1‑150 (MQ=60)
                                                              GCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCT                                                                                        <  SRR19973066.3553446/150‑1 (MQ=60)
                                                                  TGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAA                                                                                    >  SRR19973066.3500572/1‑150 (MQ=60)
                                                                  TGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAA                                                                                    <  SRR19973066.2776943/150‑1 (MQ=60)
                                                                  TGCTAATGTCGGCGAGATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAA                                                                                    <  SRR19973066.2149803/150‑1 (MQ=60)
                                                                   GCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAC                                                                                   <  SRR19973066.2460020/150‑1 (MQ=60)
                                                                     TAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAT                                                                                 <  SRR19973066.1228619/150‑1 (MQ=60)
                                                                     TAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAT                                                                                 <  SRR19973066.1093484/150‑1 (MQ=60)
                                                                      AATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATC                                                                                >  SRR19973066.124193/1‑150 (MQ=60)
                                                                          TCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCG                                                                            <  SRR19973066.259069/150‑1 (MQ=60)
                                                                           CGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGC                                                                           >  SRR19973066.1985408/1‑150 (MQ=60)
                                                                              CGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAT                                                                        >  SRR19973066.174821/1‑150 (MQ=60)
                                                                               GATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATG                                                                       <  SRR19973066.1157603/150‑1 (MQ=60)
                                                                                     AGCCGTATTACCGACTATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCTGCTATGGCTGTAACATCCGCGCGATGTACCAT                                                                 <  SRR19973066.908566/150‑1 (MQ=60)
                                                                                     AGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAT                                                                 <  SRR19973066.1013133/150‑1 (MQ=60)
                                                                                       CCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGC                                                               >  SRR19973066.693168/1‑150 (MQ=60)
                                                                                       CCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGC                                                               >  SRR19973066.692342/1‑150 (MQ=60)
                                                                                       CCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGC                                                               >  SRR19973066.731804/1‑150 (MQ=60)
                                                                                         GTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGG                                                             <  SRR19973066.2518373/150‑1 (MQ=60)
                                                                                         GTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGG                                                             >  SRR19973066.2623361/1‑150 (MQ=60)
                                                                                          TATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGC                                                            >  SRR19973066.3049317/1‑150 (MQ=60)
                                                                                          TATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGC                                                            >  SRR19973066.649390/1‑150 (MQ=60)
                                                                                          TATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAACCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGC                                                            >  SRR19973066.649367/1‑150 (MQ=60)
                                                                                           ATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCG                                                           >  SRR19973066.154341/1‑150 (MQ=60)
                                                                                               CCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAG                                                       <  SRR19973066.2571782/150‑1 (MQ=60)
                                                                                               CCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAG                                                       <  SRR19973066.2571783/150‑1 (MQ=60)
                                                                                                CGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGC                                                      <  SRR19973066.430275/150‑1 (MQ=60)
                                                                                                 GACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCG                                                     <  SRR19973066.912985/150‑1 (MQ=60)
                                                                                                 GACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCG                                                     <  SRR19973066.3071071/150‑1 (MQ=60)
                                                                                                   CGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCAGATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGTCTATGGATGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCT                                                   <  SRR19973066.627074/150‑1 (MQ=60)
                                                                                                    GATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTG                                                  >  SRR19973066.727512/1‑150 (MQ=60)
                                                                                                    GATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAACGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTG                                                  <  SRR19973066.2331586/150‑1 (MQ=60)
                                                                                                       CCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGAT                                               <  SRR19973066.2152528/150‑1 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                           >  SRR19973066.778117/1‑150 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                           <  SRR19973066.2208145/150‑1 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGACGCTGGGCAATGAATCCGGCTAAGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGAGCTGGATGACA                                           >  SRR19973066.2595065/1‑150 (MQ=60)
                                                                                                             TGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACG                                         >  SRR19973066.779460/1‑150 (MQ=60)
                                                                                                                    AAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGG                                  >  SRR19973066.2755559/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                                >  SRR19973066.562834/1‑150 (MQ=60)
                                                                                                                        CTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGACTGGTGCA                              >  SRR19973066.1710317/1‑150 (MQ=60)
                                                                                                                          ACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATT                            <  SRR19973066.1189457/150‑1 (MQ=60)
                                                                                                                             TCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACG                         >  SRR19973066.2892669/1‑150 (MQ=60)
                                                                                                                              CGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCTTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGA                        >  SRR19973066.540227/1‑150 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                    >  SRR19973066.1033954/1‑150 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                    <  SRR19973066.2102500/150‑1 (MQ=60)
                                                                                                                                   GCATTGTTCGCCATATCCTCGCGCAGAAAAAACATCCGTCGATCATAATATGTTCGCTTGGCAATGAATCCGGCTATGGCTTTAACATAAGCGCGATTTACCAAGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAG                   >  SRR19973066.3336044/1‑150 (MQ=60)
                                                                                                                                      TTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATC                <  SRR19973066.2360673/150‑1 (MQ=60)
                                                                                                                                      TTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATC                <  SRR19973066.2533596/150‑1 (MQ=60)
                                                                                                                                      TTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATC                <  SRR19973066.1628686/150‑1 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG               >  SRR19973066.3514677/1‑150 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG               >  SRR19973066.1719664/1‑150 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG               >  SRR19973066.1719656/1‑150 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG               <  SRR19973066.3084368/150‑1 (MQ=60)
                                                                                                                                         TTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCG             <  SRR19973066.485179/150‑1 (MQ=60)
                                                                                                                                         TTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCG             >  SRR19973066.952048/1‑150 (MQ=60)
                                                                                                                                          TCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGA            >  SRR19973066.2039135/1‑150 (MQ=60)
                                                                                                                                           CGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAT           >  SRR19973066.3022098/1‑150 (MQ=60)
                                                                                                                                           CGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAT           <  SRR19973066.2286052/150‑1 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT        >  SRR19973066.2889142/1‑150 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT        >  SRR19973066.3367324/1‑150 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGATCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT        <  SRR19973066.1722683/150‑1 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGATCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT        <  SRR19973066.1722717/150‑1 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       <  SRR19973066.15240/150‑1 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       <  SRR19973066.1286525/150‑1 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       >  SRR19973066.254486/1‑150 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       <  SRR19973066.3139998/150‑1 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       >  SRR19973066.1112415/1‑150 (MQ=60)
                                                                                                                                               ATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG       >  SRR19973066.122566/1‑150 (MQ=60)
                                                                                                                                                   CCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGT   <  SRR19973066.414167/150‑1 (MQ=60)
                                                                                                                                                   CCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGT   <  SRR19973066.414160/150‑1 (MQ=60)
                                                                                                                                                    CTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACTTGCGTCTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  <  SRR19973066.2637186/150‑1 (MQ=60)
                                                                                                                                                    CTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  <  SRR19973066.1865763/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑