Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,224,927 C→A 66.1% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,224,9270CA66.1% 262.5 / inf 284intergenic (+329/‑97)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (35/61);  new base A (89/99);  total (124/160)
Fisher's exact test for biased strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-01

Only 100 of 285 total aligned reads displayed.

AGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT  >  CP009273/3224779‑3225072
                                                                                                                                                    |                                                                                                                                                 
   gTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                               >  1:2912049/1‑150 (MQ=255)
       cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGCTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAccc                                                                                                                                           >  2:5068745/1‑150 (MQ=255)
        aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAAcccc                                                                                                                                          <  2:4051707/150‑1 (MQ=255)
           cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCa                                                                                                                                       <  2:3050812/150‑1 (MQ=255)
           cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCa                                                                                                                                       >  2:2356836/1‑150 (MQ=255)
            ggCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAc                                                                                                                                      <  1:3735490/150‑1 (MQ=255)
            ggCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAc                                                                                                                                      <  1:3735420/150‑1 (MQ=255)
                 cTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTcccc                                                                                                                                 >  1:3767193/1‑150 (MQ=255)
                  tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCccct                                                                                                                                >  1:1243159/1‑150 (MQ=255)
                   gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCccctt                                                                                                                               >  2:2152895/1‑150 (MQ=255)
                    cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttt                                                                                                                              >  1:3003212/1‑150 (MQ=255)
                     aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                             >  2:2939674/1‑150 (MQ=255)
                     aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                             <  2:861223/150‑1 (MQ=255)
                      ccGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttc                                                                                                                            >  2:2018622/1‑150 (MQ=255)
                          gcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccctt                                                                                                                        <  1:2253248/150‑1 (MQ=255)
                          gcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                        >  2:491249/1‑150 (MQ=255)
                                ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACCAACCCCGCACTCCCCTTTTCCCTTTTCTcc                                                                                                                  >  1:259495/1‑150 (MQ=255)
                                  cGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCgg                                                                                                                <  1:1801258/150‑1 (MQ=255)
                                     aTGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGa                                                                                                             >  2:4532472/1‑150 (MQ=255)
                                     aTGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAACCCGCACTCCCCTTTTCCCTTTTCTCCGGCGa                                                                                                             >  1:2685205/1‑150 (MQ=255)
                                         ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                         >  2:3119793/1‑150 (MQ=255)
                                          gcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCt                                                                                                        <  1:4216855/150‑1 (MQ=255)
                                           cgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTa                                                                                                       <  1:587722/150‑1 (MQ=255)
                                            gTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                      >  2:3565301/1‑150 (MQ=255)
                                            gTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                      <  1:559336/150‑1 (MQ=255)
                                               gACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAtt                                                                                                   >  1:4045507/1‑150 (MQ=255)
                                                   ccTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                               >  2:1689307/1‑150 (MQ=255)
                                                    cTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAc                                                                                              <  1:2538773/150‑1 (MQ=255)
                                                    cTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAc                                                                                              >  2:2819236/1‑150 (MQ=255)
                                                    cTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAc                                                                                              <  1:1561911/150‑1 (MQ=255)
                                                      tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                            <  1:3451147/150‑1 (MQ=255)
                                                      tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                            >  2:563097/1‑150 (MQ=255)
                                                         ccGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATc                                                                                         >  2:2223326/1‑150 (MQ=255)
                                                         ccGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATc                                                                                         >  2:2505184/1‑150 (MQ=255)
                                                          cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATcc                                                                                        <  1:2205639/150‑1 (MQ=255)
                                                           gCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCg                                                                                       <  2:1823768/150‑1 (MQ=255)
                                                           gCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCg                                                                                       <  2:2370108/150‑1 (MQ=255)
                                                            cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                      <  1:623020/150‑1 (MQ=255)
                                                            cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                      >  2:15298/1‑150 (MQ=255)
                                                             ccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAc                                                                                     <  1:873868/150‑1 (MQ=255)
                                                             ccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAc                                                                                     <  2:4261437/150‑1 (MQ=255)
                                                              cTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAcc                                                                                    <  2:276511/150‑1 (MQ=255)
                                                              cTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAcc                                                                                    >  1:610378/1‑150 (MQ=255)
                                                              cTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAcc                                                                                    <  2:2889936/150‑1 (MQ=255)
                                                                  aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAt                                                                                <  2:26572/150‑1 (MQ=255)
                                                                  aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAt                                                                                >  2:2282638/1‑150 (MQ=255)
                                                                  aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAt                                                                                <  2:1206777/150‑1 (MQ=255)
                                                                        aaTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAat                                                                          <  2:1983404/150‑1 (MQ=255)
                                                                          ttCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAatta                                                                        >  2:1736010/1‑150 (MQ=255)
                                                                          ttCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTGTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAatta                                                                        <  1:5168179/150‑1 (MQ=255)
                                                                             cGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAttt                                                                     <  1:3565301/150‑1 (MQ=255)
                                                                              gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAtttt                                                                    >  1:3469001/1‑150 (MQ=255)
                                                                               tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATACCAATTATTTTa                                                                   >  2:5103685/1‑150 (MQ=255)
                                                                               tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTa                                                                   <  2:3422670/150‑1 (MQ=255)
                                                                                aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                  <  1:1395014/150‑1 (MQ=255)
                                                                                aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                  <  2:1379356/150‑1 (MQ=255)
                                                                                aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                  <  2:3975713/150‑1 (MQ=255)
                                                                                 aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                 <  2:1443184/150‑1 (MQ=255)
                                                                                 aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                 <  1:3276745/150‑1 (MQ=255)
                                                                                 aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                 >  1:2655265/1‑150 (MQ=255)
                                                                                 aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                 >  1:3098692/1‑150 (MQ=255)
                                                                                  ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTCTTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACAt                                                                >  2:4246812/1‑150 (MQ=255)
                                                                                  ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCGCCTTTTCCCTTTTCTCCGGCGACGGCTCCATTAGAACGCATCCGACCACAGAACACTTATGTTAcgt                                                                >  1:4770758/1‑148 (MQ=255)
                                                                                   cTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATa                                                               <  1:3606134/150‑1 (MQ=255)
                                                                                       aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                           <  2:3917529/150‑1 (MQ=255)
                                                                                         aaaaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAc                                                         <  1:4631978/150‑1 (MQ=255)
                                                                                          aaaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACa                                                        <  2:3839091/150‑1 (MQ=255)
                                                                                           aaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACaa                                                       >  2:1100571/1‑150 (MQ=255)
                                                                                            aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTGTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAt                                                      <  2:4722661/150‑1 (MQ=255)
                                                                                             cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAtt                                                     >  1:2386506/1‑150 (MQ=255)
                                                                                              gcgACACGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAttt                                                    <  1:2335210/150‑1 (MQ=255)
                                                                                              gcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAACCCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACATACTGGACAAttt                                                    <  1:2335173/150‑1 (MQ=255)
                                                                                               cgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAtttt                                                   <  1:1078746/150‑1 (MQ=255)
                                                                                                 aCAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTAt                                                 <  2:353233/150‑1 (MQ=255)
                                                                                                  cAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                                <  2:5114479/150‑1 (MQ=255)
                                                                                                    aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAg                                              >  1:837413/1‑150 (MQ=255)
                                                                                                      cGTCGCATCCGGCGTGATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTCTCCCTTTTCTCCGGAGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCt                                            <  2:3768746/150‑1 (MQ=255)
                                                                                                       gTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTa                                           >  2:592198/1‑150 (MQ=255)
                                                                                                       gTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAATGGACAATTTTATGAGCTa                                           >  1:499966/1‑150 (MQ=255)
                                                                                                        tCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAc                                          <  1:4292860/150‑1 (MQ=255)
                                                                                                         cGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAcc                                         <  2:1872107/150‑1 (MQ=255)
                                                                                                         cGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCCAAATTAGAACTCATCCGACCACATAACAATTATTTTAAATACTTGACAATTTTATGTGCTAcc                                         >  1:3950562/1‑150 (MQ=255)
                                                                                                          gCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAccc                                        >  2:3335981/1‑150 (MQ=255)
                                                                                                           cATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAATGGACAATTTTATGAGCTACCCg                                       >  2:4365908/1‑150 (MQ=255)
                                                                                                            aTCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGt                                      <  1:3065491/150‑1 (MQ=255)
                                                                                                             tCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTc                                     <  1:3776627/150‑1 (MQ=255)
                                                                                                             tCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTc                                     <  1:1289995/150‑1 (MQ=255)
                                                                                                              ccGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCg                                    <  2:775869/150‑1 (MQ=255)
                                                                                                              ccGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCg                                    <  2:775559/150‑1 (MQ=255)
                                                                                                                ggCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCt                                  <  1:2665415/150‑1 (MQ=255)
                                                                                                                  cGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGt                                <  1:4816440/150‑1 (MQ=255)
                                                                                                                   gTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                               <  2:4648862/150‑1 (MQ=255)
                                                                                                                   gTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                               >  2:4481130/1‑150 (MQ=255)
                                                                                                                      aTCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATTAGCTACCCGTCGCTGTTAGc                            >  1:681399/1‑150 (MQ=255)
                                                                                                                            gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCt                      <  1:491187/150‑1 (MQ=255)
                                                                                                                            gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTAGCCCCGCt                      >  1:4382708/1‑150 (MQ=255)
                                                                                                                                gTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                  >  2:915596/1‑150 (MQ=255)
                                                                                                                                gTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                  <  1:1855437/150‑1 (MQ=255)
                                                                                                                                  aTTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt                <  2:2549139/150‑1 (MQ=255)
                                                                                                                                               cccATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACg   >  2:2637113/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT  >  CP009273/3224779‑3225072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT  >  CP009273/3224782‑3225072
                                                                                                                                                 |                                                                                                                                                 
GTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAA                                                                                                                                               >  SRR19973083.3046410/1‑150 (MQ=60)
GTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAA                                                                                                                                               >  SRR19973083.424149/1‑150 (MQ=60)
GTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAA                                                                                                                                               >  SRR19973083.2926300/1‑150 (MQ=60)
        CGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCA                                                                                                                                       <  SRR19973083.409818/150‑1 (MQ=60)
         GGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAC                                                                                                                                      <  SRR19973083.3752443/150‑1 (MQ=60)
         GGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAC                                                                                                                                      <  SRR19973083.3752373/150‑1 (MQ=60)
         GGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAC                                                                                                                                      <  SRR19973083.360138/150‑1 (MQ=60)
         GGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAC                                                                                                                                      <  SRR19973083.3752375/150‑1 (MQ=60)
          GCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACT                                                                                                                                     <  SRR19973083.2741892/150‑1 (MQ=60)
              CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCC                                                                                                                                 >  SRR19973083.3254921/1‑150 (MQ=60)
              CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCC                                                                                                                                 >  SRR19973083.3784227/1‑150 (MQ=60)
              CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCC                                                                                                                                 >  SRR19973083.3784232/1‑150 (MQ=60)
              CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCC                                                                                                                                 >  SRR19973083.3784233/1‑150 (MQ=60)
              CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCC                                                                                                                                 >  SRR19973083.3784380/1‑150 (MQ=60)
               TGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCT                                                                                                                                >  SRR19973083.1249234/1‑150 (MQ=60)
               TGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCT                                                                                                                                >  SRR19973083.2676936/1‑150 (MQ=60)
                 CACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTT                                                                                                                              >  SRR19973083.3017720/1‑150 (MQ=60)
                 CACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTT                                                                                                                              <  SRR19973083.3106262/150‑1 (MQ=60)
                       GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                        <  SRR19973083.2871535/150‑1 (MQ=60)
                       GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                        <  SRR19973083.607501/150‑1 (MQ=60)
                       GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                        <  SRR19973083.607500/150‑1 (MQ=60)
                       GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                        <  SRR19973083.2264724/150‑1 (MQ=60)
                            TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                   <  SRR19973083.3762514/150‑1 (MQ=60)
                             GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACCAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                  >  SRR19973083.260874/1‑150 (MQ=60)
                               CGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGG                                                                                                                <  SRR19973083.1810286/150‑1 (MQ=60)
                                 GATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCG                                                                                                              <  SRR19973083.2357277/150‑1 (MQ=60)
                                  ATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGA                                                                                                             >  SRR19973083.2698664/1‑150 (MQ=60)
                                  ATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGA                                                                                                             <  SRR19973083.2348954/150‑1 (MQ=60)
                                  ATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAACCCGCACTCCCCTTTTCCCTTTTCTCCGGCGA                                                                                                             >  SRR19973083.2698667/1‑150 (MQ=60)
                                      GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                         >  SRR19973083.358485/1‑150 (MQ=60)
                                      GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                         <  SRR19973083.1843066/150‑1 (MQ=60)
                                       GCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCT                                                                                                        <  SRR19973083.4235358/150‑1 (MQ=60)
                                        CGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTA                                                                                                       <  SRR19973083.590677/150‑1 (MQ=60)
                                         GTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAA                                                                                                      <  SRR19973083.562128/150‑1 (MQ=60)
                                         GTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAA                                                                                                      >  SRR19973083.3013252/1‑150 (MQ=60)
                                          TGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAA                                                                                                     >  SRR19973083.4367427/1‑150 (MQ=60)
                                           GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                    >  SRR19973083.1270758/1‑150 (MQ=60)
                                           GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                    <  SRR19973083.567943/150‑1 (MQ=60)
                                           GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                    <  SRR19973083.3329470/150‑1 (MQ=60)
                                           GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                    <  SRR19973083.568194/150‑1 (MQ=60)
                                           GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                    >  SRR19973083.1270695/1‑150 (MQ=60)
                                            GACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATT                                                                                                   >  SRR19973083.4063483/1‑150 (MQ=60)
                                            GACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATT                                                                                                   <  SRR19973083.494/150‑1 (MQ=60)
                                            GACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATT                                                                                                   <  SRR19973083.495/150‑1 (MQ=60)
                                             ACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTA                                                                                                  >  SRR19973083.830932/1‑150 (MQ=60)
                                              CGCCTTATCCGCCCTACATGTGCAAGTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                 <  SRR19973083.5021109/150‑1 (MQ=60)
                                                 CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                              <  SRR19973083.2551650/150‑1 (MQ=60)
                                                 CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                              <  SRR19973083.1569758/150‑1 (MQ=60)
                                                   TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTC                                                                                            <  SRR19973083.3467044/150‑1 (MQ=60)
                                                   TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTC                                                                                            <  SRR19973083.3466985/150‑1 (MQ=60)
                                                       CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                        <  SRR19973083.1808131/150‑1 (MQ=60)
                                                       CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                        <  SRR19973083.2216731/150‑1 (MQ=60)
                                                        GCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCG                                                                                       >  SRR19973083.3637628/1‑150 (MQ=60)
                                                         CCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGA                                                                                      <  SRR19973083.626151/150‑1 (MQ=60)
                                                         CCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGA                                                                                      <  SRR19973083.626140/150‑1 (MQ=60)
                                                          CCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                     <  SRR19973083.877947/150‑1 (MQ=60)
                                                          CCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                     <  SRR19973083.877897/150‑1 (MQ=60)
                                                           CTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACC                                                                                    <  SRR19973083.365554/150‑1 (MQ=60)
                                                           CTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACC                                                                                    >  SRR19973083.613442/1‑150 (MQ=60)
                                                           CTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACC                                                                                    >  SRR19973083.613468/1‑150 (MQ=60)
                                                            TACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAACCGACCA                                                                                   >  SRR19973083.133816/1‑150 (MQ=60)
                                                             ACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCAC                                                                                  >  SRR19973083.115746/1‑150 (MQ=60)
                                                              CATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACA                                                                                 >  SRR19973083.2293451/1‑150 (MQ=60)
                                                              CATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACA                                                                                 <  SRR19973083.4835626/150‑1 (MQ=60)
                                                              CATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACA                                                                                 <  SRR19973083.4835960/150‑1 (MQ=60)
                                                                TGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATA                                                                               <  SRR19973083.3983690/150‑1 (MQ=60)
                                                                 GTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAA                                                                              <  SRR19973083.2607798/150‑1 (MQ=60)
                                                                 GTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGATAAATTAGAACTCATCCGAACACATAA                                                                              <  SRR19973083.3061706/150‑1 (MQ=60)
                                                                   GCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                            <  SRR19973083.1335947/150‑1 (MQ=60)
                                                                    CAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAA                                                                           >  SRR19973083.5123329/1‑150 (MQ=60)
                                                                    CAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAA                                                                           <  SRR19973083.1439035/150‑1 (MQ=60)
                                                                     AATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAAT                                                                          <  SRR19973083.2990582/150‑1 (MQ=60)
                                                                       TTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTGTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTA                                                                        <  SRR19973083.5190099/150‑1 (MQ=60)
                                                                          CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                     <  SRR19973083.3581662/150‑1 (MQ=60)
                                                                           GTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                    <  SRR19973083.3530921/150‑1 (MQ=60)
                                                                           GTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                    >  SRR19973083.3484864/1‑150 (MQ=60)
                                                                           GTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                    >  SRR19973083.3484664/1‑150 (MQ=60)
                                                                            TAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAGTTTA                                                                   >  SRR19973083.5191508/1‑150 (MQ=60)
                                                                             AACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAC                                                                  <  SRR19973083.1401974/150‑1 (MQ=60)
                                                                              ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                 <  SRR19973083.3292185/150‑1 (MQ=60)
                                                                              ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                 >  SRR19973083.2668592/1‑150 (MQ=60)
                                                                              ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                 >  SRR19973083.3113539/1‑150 (MQ=60)
                                                                               CCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCGCCTTTTCCCTTTTCTCCGGCGACGGCTCCATTAGAACGCATCCGACCACAGAACACTTATGTTACGT                                                                >  SRR19973083.4791084/1‑150 (MQ=60)
                                                                                CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATA                                                               >  SRR19973083.4113041/1‑150 (MQ=60)
                                                                                CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATA                                                               <  SRR19973083.2421676/150‑1 (MQ=60)
                                                                                CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATA                                                               <  SRR19973083.3622649/150‑1 (MQ=60)
                                                                                CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATA                                                               >  SRR19973083.354911/1‑150 (MQ=60)
                                                                                   GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTG                                                            <  SRR19973083.2680404/150‑1 (MQ=60)
                                                                                     TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGA                                                          <  SRR19973083.702158/150‑1 (MQ=60)
                                                                                      AAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAC                                                         <  SRR19973083.4651956/150‑1 (MQ=60)
                                                                                        AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCGCCTTTTCCCTTTTCTACGGCGACGGCGAAATTAGAAATCATCCGCCCACATAACAATTATTTTACATACTGGACAC                                                       >  SRR19973083.1456298/1‑150 (MQ=60)
                                                                                        AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAA                                                       <  SRR19973083.2158083/150‑1 (MQ=60)
                                                                                         ACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAT                                                      >  SRR19973083.2575543/1‑150 (MQ=60)
                                                                                          CGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATT                                                     >  SRR19973083.4777628/1‑150 (MQ=60)
                                                                                          CGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATT                                                     >  SRR19973083.2398784/1‑150 (MQ=60)
                                                                                           GCGACACGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTT                                                    <  SRR19973083.2347199/150‑1 (MQ=60)
                                                                                           GCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAACCCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACATACTGGACAATTT                                                    <  SRR19973083.2347162/150‑1 (MQ=60)
                                                                                           GCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTT                                                    <  SRR19973083.2347185/150‑1 (MQ=60)
                                                                                           GCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTT                                                    <  SRR19973083.2347184/150‑1 (MQ=60)
                                                                                           GCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTT                                                    <  SRR19973083.2347112/150‑1 (MQ=60)
                                                                                            CGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTT                                                   <  SRR19973083.1084178/150‑1 (MQ=60)
                                                                                            CGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTT                                                   <  SRR19973083.967405/150‑1 (MQ=60)
                                                                                             GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTA                                                  <  SRR19973083.5062274/150‑1 (MQ=60)
                                                                                              ACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTAT                                                 <  SRR19973083.4597968/150‑1 (MQ=60)
                                                                                                 AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                              >  SRR19973083.841373/1‑150 (MQ=60)
                                                                                                    GTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTA                                           <  SRR19973083.1749939/150‑1 (MQ=60)
                                                                                                    GTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAATGGACAATTTTATGAGCTA                                           >  SRR19973083.502380/1‑150 (MQ=60)
                                                                                                     TCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAC                                          <  SRR19973083.4311548/150‑1 (MQ=60)
                                                                                                     TCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAC                                          <  SRR19973083.4310839/150‑1 (MQ=60)
                                                                                                     TCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAC                                          >  SRR19973083.643989/1‑150 (MQ=60)
                                                                                                      CGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACC                                         >  SRR19973083.1816739/1‑150 (MQ=60)
                                                                                                      CGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCCAAATTAGAACTCATCCGACCACATAACAATTATTTTAAATACTTGACAATTTTATGTGCTACC                                         >  SRR19973083.3968138/1‑150 (MQ=60)
                                                                                                         ATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGT                                      <  SRR19973083.3080187/150‑1 (MQ=60)
                                                                                                          TCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTC                                     <  SRR19973083.3793682/150‑1 (MQ=60)
                                                                                                          TCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTC                                     <  SRR19973083.3793884/150‑1 (MQ=60)
                                                                                                          TCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTC                                     <  SRR19973083.1296224/150‑1 (MQ=60)
                                                                                                           CCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCG                                    <  SRR19973083.3901406/150‑1 (MQ=60)
                                                                                                           CCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCG                                    >  SRR19973083.3112263/1‑150 (MQ=60)
                                                                                                             GGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCT                                  <  SRR19973083.2678291/150‑1 (MQ=60)
                                                                                                             GGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCT                                  <  SRR19973083.2678793/150‑1 (MQ=60)
                                                                                                               CGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGT                                <  SRR19973083.4837087/150‑1 (MQ=60)
                                                                                                                GTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTT                               >  SRR19973083.344638/1‑150 (MQ=60)
                                                                                                                GTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTGTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTT                               <  SRR19973083.4528300/150‑1 (MQ=60)
                                                                                                                   ATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATTAGCTACCCGTCGCTGTTAGC                            >  SRR19973083.684804/1‑150 (MQ=60)
                                                                                                                    TCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCC                           <  SRR19973083.4048619/150‑1 (MQ=60)
                                                                                                                         GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCT                      <  SRR19973083.493618/150‑1 (MQ=60)
                                                                                                                         GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCT                      <  SRR19973083.493556/150‑1 (MQ=60)
                                                                                                                         GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTAGCCCCGCT                      >  SRR19973083.4401756/1‑150 (MQ=60)
                                                                                                                            CGTATTCTTTTTGAATCCCATCACAACCCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGA                   <  SRR19973083.92790/150‑1 (MQ=60)
                                                                                                                             GTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAT                  <  SRR19973083.1864812/150‑1 (MQ=60)
                                                                                                                               ATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTT                <  SRR19973083.683801/150‑1 (MQ=60)
                                                                                                                               AGTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAAAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTT                <  SRR19973083.4783006/150‑1 (MQ=60)
                                                                                                                                  CTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGG             <  SRR19973083.2234573/150‑1 (MQ=60)
                                                                                                                                  CTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGG             <  SRR19973083.2517950/150‑1 (MQ=60)
                                                                                                                                  CTCTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGG             <  SRR19973083.2234627/150‑1 (MQ=60)
                                                                                                                                             CCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT  >  SRR19973083.265292/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                 
GTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT  >  CP009273/3224782‑3225072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑