Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,224,927 C→A 100% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,224,9270CA100.0% 635.6 / ‑6.7 198intergenic (+329/‑97)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (77/120);  total (77/121)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

TCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224783‑3225071
                                                                                                                                                |                                                                                                                                                
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGTCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              <  2:2236106/148‑1 (MQ=255)
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              <  1:2290342/148‑1 (MQ=255)
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              <  2:802358/148‑1 (MQ=255)
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              >  2:3160749/1‑148 (MQ=255)
   cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAc                                                                                                                                            <  1:267973/148‑1 (MQ=255)
   cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAc                                                                                                                                            >  1:2695341/1‑148 (MQ=255)
   cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAc                                                                                                                                            <  2:231764/148‑1 (MQ=255)
    aTCCGGCATGTGCTCCGCGCCTCTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                           <  2:2635055/148‑1 (MQ=255)
    aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                           <  2:292468/148‑1 (MQ=255)
     tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAATCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcac                                                                                                                                          >  1:1372651/1‑146 (MQ=255)
        ggCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGc                                                                                                                                       >  1:1852163/1‑148 (MQ=255)
           aTCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACt                                                                                                                                    <  2:187894/148‑1 (MQ=255)
            tCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTc                                                                                                                                   <  2:2520746/148‑1 (MQ=255)
             cTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcc                                                                                                                                  >  2:2410287/1‑148 (MQ=255)
                cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTGTGAATCCCATAACAAACCCCGCACTCccct                                                                                                                               <  2:3438019/148‑1 (MQ=255)
                  ccGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttt                                                                                                                             <  1:3313632/148‑1 (MQ=255)
                  ccGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttt                                                                                                                             <  1:702568/148‑1 (MQ=255)
                     cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcc                                                                                                                          <  2:225774/148‑1 (MQ=255)
                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  1:1055737/148‑1 (MQ=255)
                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  1:2450135/148‑1 (MQ=255)
                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  1:2606531/148‑1 (MQ=255)
                        cagtGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAATCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                       <  2:2553197/145‑1 (MQ=255)
                          cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  2:2740177/148‑1 (MQ=255)
                          cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  1:1963442/148‑1 (MQ=255)
                            ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtct                                                                                                                   <  1:657815/148‑1 (MQ=255)
                            ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtct                                                                                                                   <  1:2035000/148‑1 (MQ=255)
                            ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtct                                                                                                                   <  1:2581560/148‑1 (MQ=255)
                             gCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                  <  1:3341433/148‑1 (MQ=255)
                               ggATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCg                                                                                                                >  1:2755561/1‑148 (MQ=255)
                                    cggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGAc                                                                                                           <  2:1852163/148‑1 (MQ=255)
                                         tGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTa                                                                                                      >  1:1522226/1‑148 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     >  1:1703332/1‑148 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  2:2776319/148‑1 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  1:509733/148‑1 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  1:3492985/148‑1 (MQ=255)
                                           gACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaaa                                                                                                    >  2:694534/1‑148 (MQ=255)
                                              gCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTa                                                                                                 <  1:2112074/148‑1 (MQ=255)
                                               ccTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg                                                                                                >  1:443369/1‑148 (MQ=255)
                                                cTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa                                                                                               <  1:3256834/148‑1 (MQ=255)
                                                cTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGa                                                                                               <  1:798947/148‑1 (MQ=255)
                                                 ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                              <  1:2165872/148‑1 (MQ=255)
                                                 ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                              <  1:3614403/148‑1 (MQ=255)
                                                   aTCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACt                                                                                            >  2:1262572/1‑148 (MQ=255)
                                                    tCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                           <  2:1318917/148‑1 (MQ=255)
                                                      cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:585889/148‑1 (MQ=255)
                                                         ccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCg                                                                                      <  1:1313760/148‑1 (MQ=255)
                                                           tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAc                                                                                    <  2:2276379/148‑1 (MQ=255)
                                                           tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAc                                                                                    >  2:2851876/1‑148 (MQ=255)
                                                            aCATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAcc                                                                                   >  2:140550/1‑148 (MQ=255)
                                                                  gCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATaa                                                                             >  1:2327547/1‑148 (MQ=255)
                                                                     aTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACaa                                                                          >  2:2632021/1‑148 (MQ=255)
                                                                         cGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattat                                                                      <  1:735723/148‑1 (MQ=255)
                                                                           tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAttt                                                                    >  2:1471296/1‑148 (MQ=255)
                                                                            aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAtttt                                                                   <  1:1892980/148‑1 (MQ=255)
                                                                            aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAtttt                                                                   <  1:1749607/148‑1 (MQ=255)
                                                                            aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAattt                                                                   <  2:3244199/148‑1 (MQ=255)
                                                                             aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTa                                                                  <  2:3661603/148‑1 (MQ=255)
                                                                                tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCAAGCCCCTTTTCCCTTTTCTCCGGCGACCGCTCAAATAGAACTCATCCGACCACAAAACAATTATTTTACaa                                                               >  1:2979636/1‑147 (MQ=255)
                                                                                 ggATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATa                                                              <  1:2880707/148‑1 (MQ=255)
                                                                                 ggATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATa                                                              >  1:612108/1‑148 (MQ=255)
                                                                                  gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAc                                                             >  1:3051426/1‑148 (MQ=255)
                                                                                  gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAc                                                             <  1:1483089/148‑1 (MQ=255)
                                                                                    tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTg                                                           >  1:1899165/1‑148 (MQ=255)
                                                                                    tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTg                                                           <  1:3460501/148‑1 (MQ=255)
                                                                                     aaaaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                          <  1:3292508/148‑1 (MQ=255)
                                                                                        aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACa                                                       >  2:3352036/1‑148 (MQ=255)
                                                                                         cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACaa                                                      <  2:746127/148‑1 (MQ=255)
                                                                                            gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAttt                                                   <  2:772069/148‑1 (MQ=255)
                                                                                             aCAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAtttt                                                  >  1:930571/1‑148 (MQ=255)
                                                                                              cAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTa                                                 <  1:340304/148‑1 (MQ=255)
                                                                                                aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  2:1775918/148‑1 (MQ=255)
                                                                                                 gCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGa                                              >  1:2759350/1‑148 (MQ=255)
                                                                                                  cGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAg                                             <  2:1017907/148‑1 (MQ=255)
                                                                                                   gTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGc                                            <  1:1319640/148‑1 (MQ=255)
                                                                                                     cGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTa                                          >  1:2199926/1‑148 (MQ=255)
                                                                                                          ccGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGt                                     >  1:2413540/1‑148 (MQ=255)
                                                                                                            ggCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCg                                   >  2:1902248/1‑148 (MQ=255)
                                                                                                             gCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGc                                  <  2:410134/148‑1 (MQ=255)
                                                                                                                 tATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                              <  2:2971882/148‑1 (MQ=255)
                                                                                                                 tATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                              >  2:2484956/1‑148 (MQ=255)
                                                                                                                  aTCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTc                             >  2:2473496/1‑148 (MQ=255)
                                                                                                                  aTCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTc                             >  2:3691003/1‑148 (MQ=255)
                                                                                                                   tCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCg                            >  1:1722503/1‑148 (MQ=255)
                                                                                                                    cACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGc                           >  2:2042994/1‑148 (MQ=255)
                                                                                                                    cACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGc                           >  2:19680/1‑148 (MQ=255)
                                                                                                                    cACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGc                           <  2:2863722/148‑1 (MQ=255)
                                                                                                                    cACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTGTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGc                           <  2:2865146/148‑1 (MQ=255)
                                                                                                                     aCCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGcc                          <  2:1533226/148‑1 (MQ=255)
                                                                                                                            gTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTgg                   >  1:3167317/1‑148 (MQ=255)
                                                                                                                             tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  <  2:2211346/148‑1 (MQ=255)
                                                                                                                              aTTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                 <  2:2617627/148‑1 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               >  1:2792577/1‑148 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               <  1:2678910/148‑1 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               <  2:930571/148‑1 (MQ=255)
                                                                                                                                 cTTTTTGAATCCCATAACAAACCCCGCACTCCCCTGTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTa              <  2:2333148/148‑1 (MQ=255)
                                                                                                                                   ttttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAgg            >  1:1037620/1‑148 (MQ=255)
                                                                                                                                    tttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGt           >  2:1235689/1‑148 (MQ=255)
                                                                                                                                    tttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGt           >  2:2386260/1‑148 (MQ=255)
                                                                                                                                             cATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACg  <  2:3509787/148‑1 (MQ=255)
                                                                                                                                             cATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACg  <  2:3046883/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224783‑3225071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAA  >  CP009273/3224778‑3225075
                                                                                                                                                     |                                                                                                                                                    
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                      >  SRR19973069.2506510/1‑150 (MQ=60)
 AGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAA                                                                                                                                                     <  SRR19973069.967663/150‑1 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGTCGTATGCGTCGTGTAAGGATTATCCGCGCTACATGTGCATTTCCGTACCCCGGATAAAACGCGCCAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAC                                                                                                                                                <  SRR19973069.2306977/150‑1 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAC                                                                                                                                                >  SRR19973069.2713400/1‑150 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAC                                                                                                                                                <  SRR19973069.2305904/150‑1 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCC                                                                                                                                              <  SRR19973069.269842/150‑1 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCC                                                                                                                                              <  SRR19973069.269880/150‑1 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAATCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACAC                                                                                                                                              >  SRR19973069.1382210/1‑150 (MQ=60)
           CCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGC                                                                                                                                           >  SRR19973069.1865095/1‑150 (MQ=60)
                ATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCccgcc                                                                                                                                      >  SRR19973069.1747193/1‑145 (MQ=60)
                   TGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCATACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCT                                                                                                                                   <  SRR19973069.3299016/150‑1 (MQ=60)
                      ACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTT                                                                                                                                <  SRR19973069.2185570/150‑1 (MQ=60)
                       CCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTC                                                                                                                               <  SRR19973069.707511/150‑1 (MQ=60)
                       CCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTC                                                                                                                               <  SRR19973069.3335351/150‑1 (MQ=60)
                       CCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTC                                                                                                                               <  SRR19973069.3335335/150‑1 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                          <  SRR19973069.2166861/150‑1 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                          <  SRR19973069.2466618/150‑1 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                          <  SRR19973069.2623938/150‑1 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                          <  SRR19973069.2623946/150‑1 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                          <  SRR19973069.1063075/150‑1 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                       <  SRR19973069.1977062/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.661709/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.2598721/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.662243/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.2049116/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.2049130/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                     <  SRR19973069.2463458/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                    >  SRR19973069.2773956/1‑150 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                    <  SRR19973069.3363308/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                    <  SRR19973069.3366585/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                    >  SRR19973069.2773950/1‑150 (MQ=60)
                                            CGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTA                                                                                                          >  SRR19973069.605824/1‑150 (MQ=60)
                                            CGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTA                                                                                                          >  SRR19973069.1532717/1‑150 (MQ=60)
                                             GTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAA                                                                                                         >  SRR19973069.1715249/1‑150 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                       <  SRR19973069.51540/150‑1 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                       <  SRR19973069.513366/150‑1 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                       <  SRR19973069.3515500/150‑1 (MQ=60)
                                                GACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATT                                                                                                      >  SRR19973069.1378865/1‑150 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                    >  SRR19973069.446611/1‑150 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                    >  SRR19973069.446052/1‑150 (MQ=60)
                                                   GCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGA                                                                                                   <  SRR19973069.2126636/150‑1 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                                 <  SRR19973069.804612/150‑1 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                                 <  SRR19973069.3278165/150‑1 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                                 <  SRR19973069.1405233/150‑1 (MQ=60)
                                                      TTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACT                                                                                                <  SRR19973069.2180678/150‑1 (MQ=60)
                                                      TTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACT                                                                                                <  SRR19973069.3638007/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                           >  SRR19973069.270301/1‑150 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                           <  SRR19973069.3352708/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                           <  SRR19973069.2426584/150‑1 (MQ=60)
                                                             CCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGA                                                                                         >  SRR19973069.2903322/1‑150 (MQ=60)
                                                              CCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                        <  SRR19973069.1322757/150‑1 (MQ=60)
                                                              CCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                        <  SRR19973069.1323238/150‑1 (MQ=60)
                                                                     GTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAA                                                                                 >  SRR19973069.2343375/1‑150 (MQ=60)
                                                                     GTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCGAAATTAGAACTCATCCGACCACATAA                                                                                 >  SRR19973069.1543758/1‑150 (MQ=60)
                                                                       GCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                               <  SRR19973069.2835880/150‑1 (MQ=60)
                                                                          ATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATT                                                                            <  SRR19973069.1812263/150‑1 (MQ=60)
                                                                            TCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAT                                                                          <  SRR19973069.3001488/150‑1 (MQ=60)
                                                                              CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                        <  SRR19973069.740914/150‑1 (MQ=60)
                                                                              CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                        <  SRR19973069.740937/150‑1 (MQ=60)
                                                                              CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                        <  SRR19973069.741839/150‑1 (MQ=60)
                                                                               GTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                       <  SRR19973069.1224510/150‑1 (MQ=60)
                                                                                 AACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAC                                                                     <  SRR19973069.1906099/150‑1 (MQ=60)
                                                                                 AACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAC                                                                     <  SRR19973069.1761919/150‑1 (MQ=60)
                                                                                   CCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCAAGCCCCTTTTCCCTTTTCTCCGGCGACCGCTCAAATAGAACTCATCCGACCACAAAACAATTATTTTACAA                                                                   >  SRR19973069.2999416/1‑150 (MQ=60)
                                                                                    CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACCATTATTTTACATA                                                                  >  SRR19973069.2055921/1‑150 (MQ=60)
                                                                                    CTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATA                                                                  >  SRR19973069.616199/1‑150 (MQ=60)
                                                                                     TGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                 >  SRR19973069.1913244/1‑150 (MQ=60)
                                                                                     TGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                 >  SRR19973069.3071619/1‑150 (MQ=60)
                                                                                      GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACT                                                                <  SRR19973069.2899625/150‑1 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTTTTCTTTTTTTATCCCCTAAAAAACCCCGCACGCCCCATTTCCCTTTTGTCCGGCGACGGCGCAATTATAACTCAACCGACCCCATAACAATTATTTTAAATACTG                                                               >  SRR19973069.324890/1‑150 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTG                                                               >  SRR19973069.1912244/1‑150 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTG                                                               <  SRR19973069.1493402/150‑1 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAAATACTG                                                               >  SRR19973069.1912305/1‑150 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTG                                                               >  SRR19973069.1879332/1‑150 (MQ=60)
                                                                                         TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGA                                                             <  SRR19973069.3482905/150‑1 (MQ=60)
                                                                                          AAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAC                                                            <  SRR19973069.3314068/150‑1 (MQ=60)
                                                                                                CGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTT                                                      >  SRR19973069.936886/1‑150 (MQ=60)
                                                                                                  ACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCGTTTCCCTTTTCTCCGGCGACGGCGAAATTAGAAATCATCCGACCCCATAACAATTATTTTAAATACTTGACAAATTTAT                                                    >  SRR19973069.3618205/1‑150 (MQ=60)
                                                                                                   CAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATG                                                   <  SRR19973069.342693/150‑1 (MQ=60)
                                                                                                   CAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATG                                                   <  SRR19973069.342823/150‑1 (MQ=60)
                                                                                                    AAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGA                                                  >  SRR19973069.2777786/1‑150 (MQ=60)
                                                                                                    AAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGA                                                  >  SRR19973069.2744677/1‑150 (MQ=60)
                                                                                                    AAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGA                                                  >  SRR19973069.2777766/1‑150 (MQ=60)
                                                                                                      GCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGC                                                <  SRR19973069.2507614/150‑1 (MQ=60)
                                                                                                       CGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCT                                               <  SRR19973069.699409/150‑1 (MQ=60)
                                                                                                        GTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTA                                              >  SRR19973069.2214860/1‑150 (MQ=60)
                                                                                                        GTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTA                                              <  SRR19973069.1328697/150‑1 (MQ=60)
                                                                                                             ATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGT                                         >  SRR19973069.2429857/1‑150 (MQ=60)
                                                                                                                    GTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTT                                  <  SRR19973069.350042/150‑1 (MQ=60)
                                                                                                                      TATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCG                                >  SRR19973069.1734574/1‑150 (MQ=60)
                                                                                                                      TATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCG                                >  SRR19973069.1733540/1‑150 (MQ=60)
                                                                                                                          ACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCC                            <  SRR19973069.2257506/150‑1 (MQ=60)
                                                                                                                               GCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGG                       >  SRR19973069.3187955/1‑150 (MQ=60)
                                                                                                                               GCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGG                       >  SRR19973069.3187958/1‑150 (MQ=60)
                                                                                                                                  TATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATT                    >  SRR19973069.1498620/1‑150 (MQ=60)
                                                                                                                                   ATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTT                   >  SRR19973069.2811147/1‑150 (MQ=60)
                                                                                                                                     TCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAG                 <  SRR19973069.2696871/150‑1 (MQ=60)
                                                                                                                                      CTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGG                >  SRR19973069.1044824/1‑150 (MQ=60)
                                                                                                                                                CCCATAACACACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG      <  SRR19973069.86423/150‑1 (MQ=60)
                                                                                                                                                  CATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT    >  SRR19973069.638347/1‑150 (MQ=60)
                                                                                                                                                  CATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT    >  SRR19973069.638501/1‑150 (MQ=60)
                                                                                                                                                  CATAACAAACCCCGCACTCCCCATGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT    <  SRR19973069.2657912/150‑1 (MQ=60)
                                                                                                                                                   ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA   >  SRR19973069.3665775/1‑150 (MQ=60)
                                                                                                                                                    TAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTTCCACGTTAA  >  SRR19973069.3547726/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAA  >  CP009273/3224778‑3225075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑