Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,224,927 C→A 100% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,224,9270CA96.5% 914.1 / 21.9 288intergenic (+329/‑97)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base A (131/147);  total (135/153)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 296 total aligned reads displayed.

GCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT  >  CP009273/3224780‑3225073
                                                                                                                                                   |                                                                                                                                                  
gCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATaac                                                                                                                                                  <  2:974807/150‑1 (MQ=255)
 cGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATaaca                                                                                                                                                 >  2:842147/1‑150 (MQ=255)
  gTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                                >  2:2023419/1‑150 (MQ=255)
  gTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                                >  2:3419203/1‑150 (MQ=255)
  gTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                                >  2:600355/1‑150 (MQ=255)
     gCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                             >  1:2483384/1‑150 (MQ=255)
       aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcccc                                                                                                                                           <  1:5977/150‑1 (MQ=255)
                 tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccct                                                                                                                                 >  1:272609/1‑150 (MQ=255)
                  gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctt                                                                                                                                <  1:2848644/150‑1 (MQ=255)
                  gCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctt                                                                                                                                >  2:3090224/1‑150 (MQ=14)
                        cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                          <  1:1858484/150‑1 (MQ=255)
                         gcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                         >  1:1807574/1‑150 (MQ=255)
                         gcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                         >  1:3516086/1‑150 (MQ=255)
                          ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcccttt                                                                                                                        <  1:2453563/150‑1 (MQ=255)
                           cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                       >  1:3767791/1‑150 (MQ=255)
                           cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                       >  1:64721/1‑150 (MQ=255)
                             cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtct                                                                                                                     <  2:2366114/150‑1 (MQ=255)
                             cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtct                                                                                                                     <  1:4085753/150‑1 (MQ=255)
                              tGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                    <  2:2749336/150‑1 (MQ=255)
                              tGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                    >  1:262017/1‑150 (MQ=255)
                              tGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                    <  1:3928347/150‑1 (MQ=255)
                               ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTcc                                                                                                                   <  2:3848071/150‑1 (MQ=255)
                               ggCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTcc                                                                                                                   <  2:3996640/150‑1 (MQ=255)
                                gCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCg                                                                                                                  <  2:4070207/150‑1 (MQ=255)
                                gCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCg                                                                                                                  <  2:366713/150‑1 (MQ=255)
                                  ggATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc                                                                                                                >  2:4247439/1‑150 (MQ=255)
                                  ggATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc                                                                                                                >  1:375025/1‑150 (MQ=255)
                                  ggATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc                                                                                                                >  1:4374333/1‑150 (MQ=255)
                                        ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                          >  2:2716049/1‑150 (MQ=255)
                                        ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                          >  2:3531102/1‑150 (MQ=255)
                                        ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                          >  1:988305/1‑150 (MQ=255)
                                        ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                          >  1:444340/1‑150 (MQ=255)
                                           gTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                       <  1:1456481/150‑1 (MQ=255)
                                           gTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGAGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                       <  2:360056/150‑1 (MQ=255)
                                               aCGCCTTATCCGCCCTACATGTGCAATTCCGTACCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTa                                                                                                   <  2:3865260/150‑1 (MQ=255)
                                                cGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGCTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg                                                                                                  >  1:1968643/1‑150 (MQ=255)
                                                  ccTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                                >  2:4012034/1‑150 (MQ=255)
                                                    ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACt                                                                                              <  2:2951003/150‑1 (MQ=255)
                                                     tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                             >  1:4033220/1‑150 (MQ=255)
                                                     tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                             >  1:3454668/1‑150 (MQ=255)
                                                      aTCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCa                                                                                            <  2:2144768/150‑1 (MQ=255)
                                                       tCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                           <  2:2034916/150‑1 (MQ=255)
                                                         cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATcc                                                                                         <  1:534571/150‑1 (MQ=255)
                                                           cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                       <  2:719451/150‑1 (MQ=255)
                                                           cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                       >  2:4460412/1‑150 (MQ=255)
                                                           cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                       <  2:4371251/150‑1 (MQ=255)
                                                            ccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAc                                                                                      <  2:2585023/150‑1 (MQ=255)
                                                                cATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcaca                                                                                  <  2:3762792/150‑1 (MQ=255)
                                                                 aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAt                                                                                 <  1:3210163/150‑1 (MQ=255)
                                                                     gCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACa                                                                             <  2:2137090/150‑1 (MQ=255)
                                                                     gCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACa                                                                             >  1:3395544/1‑150 (MQ=255)
                                                                         ttCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAatta                                                                         >  1:1227558/1‑150 (MQ=255)
                                                                              tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCCTAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAAAACAATTATTTTa                                                                    >  2:2611188/1‑150 (MQ=255)
                                                                              tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTa                                                                    <  2:3752310/150‑1 (MQ=255)
                                                                               taCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                   <  2:4213696/149‑1 (MQ=255)
                                                                                aCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                  <  1:3418571/150‑1 (MQ=255)
                                                                                   tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAc                                                               >  1:4486564/1‑150 (MQ=255)
                                                                                    ggATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACt                                                              <  2:1520707/150‑1 (MQ=255)
                                                                                    ggATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACt                                                              >  2:26451/1‑150 (MQ=255)
                                                                                      aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                            <  1:1249257/150‑1 (MQ=255)
                                                                                      aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                            <  1:617672/150‑1 (MQ=255)
                                                                                      aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                            <  1:3334164/150‑1 (MQ=255)
                                                                                      aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                            >  2:177319/1‑150 (MQ=255)
                                                                                       tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGa                                                           >  2:3815380/1‑150 (MQ=255)
                                                                                       tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGa                                                           <  2:1081956/150‑1 (MQ=255)
                                                                                            cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAtt                                                      <  2:3007322/150‑1 (MQ=255)
                                                                                            cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAtt                                                      >  2:317134/1‑150 (MQ=255)
                                                                                               gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTa                                                   >  1:1604070/1‑150 (MQ=255)
                                                                                               gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTa                                                   >  2:493691/1‑150 (MQ=255)
                                                                                               gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTa                                                   >  2:1899926/1‑150 (MQ=255)
                                                                                                 cAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                                 <  2:2836446/150‑1 (MQ=255)
                                                                                                  aaGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTTCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGa                                                >  1:749324/1‑150 (MQ=255)
                                                                                                  aaGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGa                                                <  2:3985923/150‑1 (MQ=255)
                                                                                                  aaGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGa                                                >  2:692577/1‑150 (MQ=255)
                                                                                                     cGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCt                                             <  1:2190965/150‑1 (MQ=255)
                                                                                                     cGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCt                                             <  2:444338/150‑1 (MQ=255)
                                                                                                     cGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCt                                             <  1:3319360/150‑1 (MQ=255)
                                                                                                              cGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGc                                    >  2:4507951/1‑150 (MQ=255)
                                                                                                              cGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGc                                    >  1:3068343/1‑150 (MQ=255)
                                                                                                               ggCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCTGCGACGGCTAAATTAGAACTCATACGACCACATAACAATTTTTTTACATACTGTACAATTTTATGAGCTACCCGTCGCt                                   <  2:2226053/150‑1 (MQ=255)
                                                                                                               ggCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTGTATGAGCTACCCGTCGCt                                   >  2:1701967/1‑150 (MQ=255)
                                                                                                                 cGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAAACTGGACAATTTTATGAGCTACCCGTCGCTGt                                 >  1:688615/1‑150 (MQ=255)
                                                                                                                  gTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                                <  2:1322679/150‑1 (MQ=255)
                                                                                                                    tATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCg                              >  2:141544/1‑150 (MQ=255)
                                                                                                                      tCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGcc                            <  2:4130285/150‑1 (MQ=255)
                                                                                                                      tCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGcc                            >  2:3302823/1‑150 (MQ=255)
                                                                                                                        aCCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGcccc                          <  1:2361682/150‑1 (MQ=255)
                                                                                                                          cGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGc                        >  2:1564605/1‑150 (MQ=255)
                                                                                                                          cGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGc                        >  2:2776282/1‑150 (MQ=255)
                                                                                                                           gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCt                       <  1:4247439/150‑1 (MQ=255)
                                                                                                                           gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCt                       <  1:382135/150‑1 (MQ=255)
                                                                                                                           gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCt                       <  2:33611/150‑1 (MQ=255)
                                                                                                                              cGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                    >  2:85574/1‑150 (MQ=255)
                                                                                                                                     tttttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGt             >  2:2479228/1‑150 (MQ=255)
                                                                                                                                      ttttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGtt            <  1:1910717/150‑1 (MQ=255)
                                                                                                                                           aaTCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAcc       >  1:1330521/1‑150 (MQ=255)
                                                                                                                                            aTCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCa      <  2:124378/150‑1 (MQ=255)
                                                                                                                                             tCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCAc     <  1:3283010/150‑1 (MQ=255)
                                                                                                                                              cccATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACg    >  2:3921451/1‑150 (MQ=255)
                                                                                                                                                cATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGtt  >  2:3710194/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT  >  CP009273/3224780‑3225073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  CP009273/3224778‑3225076
                                                                                                                                                     |                                                                                                                                                     
AAGCGTCGCTTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.4453963/150‑1 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.2020593/150‑1 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.2020565/150‑1 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       >  SRR19973072.934749/1‑150 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.4188848/150‑1 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       >  SRR19973072.73700/1‑150 (MQ=60)
AAGCGTAGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.2020594/150‑1 (MQ=60)
AAGCGTAGCATCCGGCATCTGCACCGCGCCACGGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973072.4453951/150‑1 (MQ=60)
 AGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAA                                                                                                                                                      <  SRR19973072.2293706/150‑1 (MQ=60)
 AGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAACTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAA                                                                                                                                                      <  SRR19973072.2293732/150‑1 (MQ=60)
  GCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAAC                                                                                                                                                     <  SRR19973072.1652951/150‑1 (MQ=60)
   CGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACA                                                                                                                                                    <  SRR19973072.699359/150‑1 (MQ=60)
     TCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAA                                                                                                                                                  <  SRR19973072.2631721/150‑1 (MQ=60)
       GCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACC                                                                                                                                                >  SRR19973072.2488774/1‑150 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCC                                                                                                                                               <  SRR19973072.3779890/150‑1 (MQ=60)
         ATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCC                                                                                                                                              <  SRR19973072.5997/150‑1 (MQ=60)
          TCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCG                                                                                                                                             <  SRR19973072.190926/150‑1 (MQ=60)
          TCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCG                                                                                                                                             <  SRR19973072.3501272/150‑1 (MQ=60)
          TCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCC‑TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGC                                                                                                                                            >  SRR19973072.1239134/1‑150 (MQ=60)
            CGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCA                                                                                                                                           >  SRR19973072.2566104/1‑150 (MQ=60)
            CGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCA                                                                                                                                           <  SRR19973072.606655/150‑1 (MQ=60)
             GGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCAC                                                                                                                                          >  SRR19973072.1713773/1‑150 (MQ=60)
               CATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACTCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTC                                                                                                                                        >  SRR19973072.4383807/1‑150 (MQ=60)
                   TGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCT                                                                                                                                    >  SRR19973072.273248/1‑150 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                   <  SRR19973072.2854713/150‑1 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                   <  SRR19973072.2854733/150‑1 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                   <  SRR19973072.4457113/150‑1 (MQ=60)
                         GCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCC                                                                                                                              <  SRR19973072.1306220/150‑1 (MQ=60)
                          CGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCT                                                                                                                             <  SRR19973072.1862503/150‑1 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            <  SRR19973072.2348779/150‑1 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            >  SRR19973072.1811743/1‑150 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            >  SRR19973072.1811516/1‑150 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            >  SRR19973072.3523461/1‑150 (MQ=60)
                            CCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTT                                                                                                                           <  SRR19973072.2458895/150‑1 (MQ=60)
                            CAACTGGCGGATGCGGCGTGGACGCCTTATCCGCACTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAACAGTCGCATCCGCCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTCCCTTT                                                                                                                           >  SRR19973072.1045022/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                          >  SRR19973072.3775697/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                          >  SRR19973072.64860/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                          >  SRR19973072.64879/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                          >  SRR19973072.64894/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                          >  SRR19973072.3006672/1‑150 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                        <  SRR19973072.4094135/150‑1 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                        <  SRR19973072.4094266/150‑1 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                        <  SRR19973072.4094327/150‑1 (MQ=60)
                                TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                       <  SRR19973072.3933744/150‑1 (MQ=60)
                                TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                       <  SRR19973072.3936546/150‑1 (MQ=60)
                                TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                       >  SRR19973072.262626/1‑150 (MQ=60)
                                TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                       >  SRR19973072.262643/1‑150 (MQ=60)
                                 GGCGTATGCGGCGTGTACGCCTTATCCGCCCTAGATGTGCATTTCCGTATCCTGTATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                      <  SRR19973072.3630952/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                     <  SRR19973072.3121427/150‑1 (MQ=60)
                                   CGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGG                                                                                                                    >  SRR19973072.33706/1‑150 (MQ=60)
                                   CGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGG                                                                                                                    <  SRR19973072.4273511/150‑1 (MQ=60)
                                    GGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC                                                                                                                   >  SRR19973072.375837/1‑150 (MQ=60)
                                    GGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC                                                                                                                   >  SRR19973072.375856/1‑150 (MQ=60)
                                    GGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC                                                                                                                   >  SRR19973072.4383524/1‑150 (MQ=60)
                                          GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                             >  SRR19973072.445370/1‑150 (MQ=60)
                                          GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                             >  SRR19973072.990465/1‑150 (MQ=60)
                                          GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                             >  SRR19973072.445368/1‑150 (MQ=60)
                                          GGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGC                                                                                                             >  SRR19973072.4354038/1‑150 (MQ=60)
                                            CGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTA                                                                                                           >  SRR19973072.1555841/1‑150 (MQ=60)
                                             GTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAA                                                                                                          <  SRR19973072.1459609/150‑1 (MQ=60)
                                              TGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAA                                                                                                         >  SRR19973072.1440322/1‑150 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                     >  SRR19973072.1814685/1‑150 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGCTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                     >  SRR19973072.1972397/1‑150 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGCTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                     >  SRR19973072.1972858/1‑150 (MQ=60)
                                                       TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTC                                                                                                >  SRR19973072.3461849/1‑150 (MQ=60)
                                                       TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTC                                                                                                >  SRR19973072.3461910/1‑150 (MQ=60)
                                                       TATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTC                                                                                                >  SRR19973072.4041693/1‑150 (MQ=60)
                                                        ATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCA                                                                                               >  SRR19973072.3050112/1‑150 (MQ=60)
                                                         TCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAT                                                                                              >  SRR19973072.936831/1‑150 (MQ=60)
                                                          CCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATC                                                                                             <  SRR19973072.2213140/150‑1 (MQ=60)
                                                          CCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATC                                                                                             <  SRR19973072.4551252/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                            <  SRR19973072.535840/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                            <  SRR19973072.536373/150‑1 (MQ=60)
                                                            GCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCG                                                                                           <  SRR19973072.136348/150‑1 (MQ=60)
                                                                   ATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAT                                                                                    <  SRR19973072.3216949/150‑1 (MQ=60)
                                                                    TGTGCAATTCCGTAACCTGGATAAAACGTGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATA                                                                                   <  SRR19973072.3419808/150‑1 (MQ=60)
                                                                    TGTGCAATTCCGTAACCTGGATAAAACGTGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATA                                                                                   <  SRR19973072.1963585/150‑1 (MQ=60)
                                                                      TGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAAC                                                                                 <  SRR19973072.582538/150‑1 (MQ=60)
                                                                       GCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                                >  SRR19973072.3402715/1‑150 (MQ=60)
                                                                       GCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                                >  SRR19973072.3403301/1‑150 (MQ=60)
                                                                         AATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAAT                                                                              >  SRR19973072.2475739/1‑150 (MQ=60)
                                                                           TTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTA                                                                            >  SRR19973072.1230178/1‑150 (MQ=60)
                                                                              CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                         <  SRR19973072.3089919/150‑1 (MQ=60)
                                                                              CGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                         <  SRR19973072.4199258/150‑1 (MQ=60)
                                                                                TAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTA                                                                       >  SRR19973072.3328217/1‑150 (MQ=60)
                                                                                 AACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAC                                                                      <  SRR19973072.2675571/150‑1 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     <  SRR19973072.3426406/150‑1 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     <  SRR19973072.3425773/150‑1 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     <  SRR19973072.3819306/150‑1 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     >  SRR19973072.4534280/1‑150 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     <  SRR19973072.601740/150‑1 (MQ=60)
                                                                                  ACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACA                                                                     >  SRR19973072.217705/1‑150 (MQ=60)
                                                                                     TGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                  >  SRR19973072.4496020/1‑150 (MQ=60)
                                                                                     TGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCCCATAACAATTATTTTACATAC                                                                  >  SRR19973072.4496032/1‑150 (MQ=60)
                                                                                     TGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                  >  SRR19973072.2409545/1‑150 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               <  SRR19973072.1251937/150‑1 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               >  SRR19973072.3278780/1‑150 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               <  SRR19973072.619092/150‑1 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               <  SRR19973072.3341238/150‑1 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               <  SRR19973072.1251984/150‑1 (MQ=60)
                                                                                         TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGA                                                              <  SRR19973072.3217152/150‑1 (MQ=60)
                                                                                                 GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTA                                                      >  SRR19973072.1607525/1‑150 (MQ=60)
                                                                                                 GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTA                                                      >  SRR19973072.1514941/1‑150 (MQ=60)
                                                                                                    AAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTTCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGA                                                   >  SRR19973072.750921/1‑150 (MQ=60)
                                                                                                       CGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACACTTATTTTACATACTGGACAATTTTATGAGCT                                                <  SRR19973072.348356/150‑1 (MQ=60)
                                                                                                       CGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCT                                                <  SRR19973072.3325957/150‑1 (MQ=60)
                                                                                                       CGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCT                                                <  SRR19973072.3326406/150‑1 (MQ=60)
                                                                                                       CGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCT                                                <  SRR19973072.2195731/150‑1 (MQ=60)
                                                                                                              TCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACACACCCCTCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTC                                         <  SRR19973072.3993859/150‑1 (MQ=60)
                                                                                                                CGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGC                                       >  SRR19973072.3074822/1‑150 (MQ=60)
                                                                                                                 GGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCT                                      <  SRR19973072.4144443/150‑1 (MQ=60)
                                                                                                                  GCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTG                                     <  SRR19973072.278784/150‑1 (MQ=60)
                                                                                                                  GCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTG                                     <  SRR19973072.3835390/150‑1 (MQ=60)
                                                                                                                   CGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGT                                    >  SRR19973072.726854/1‑150 (MQ=60)
                                                                                                                   CGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAAACTGGACAATTTTATGAGCTACCCGTCGCTGT                                    >  SRR19973072.690137/1‑150 (MQ=60)
                                                                                                                     TTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTC                                  <  SRR19973072.3327324/150‑1 (MQ=60)
                                                                                                                          ACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCC                             <  SRR19973072.2366766/150‑1 (MQ=60)
                                                                                                                           CCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCG                            >  SRR19973072.3621456/1‑150 (MQ=60)
                                                                                                                            CGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGC                           >  SRR19973072.1897115/1‑150 (MQ=60)
                                                                                                                             GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCT                          <  SRR19973072.382963/150‑1 (MQ=60)
                                                                                                                             GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCT                          <  SRR19973072.4256275/150‑1 (MQ=60)
                                                                                                                                CGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGA                       >  SRR19973072.203165/1‑150 (MQ=60)
                                                                                                                                CGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGA                       >  SRR19973072.3368661/1‑150 (MQ=60)
                                                                                                                                        TTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTT               <  SRR19973072.1914789/150‑1 (MQ=60)
                                                                                                                                           TGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTA            <  SRR19973072.402777/150‑1 (MQ=60)
                                                                                                                                           TGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTA            >  SRR19973072.669913/1‑150 (MQ=60)
                                                                                                                                           TGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTA            >  SRR19973072.669934/1‑150 (MQ=60)
                                                                                                                                            GAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAC           <  SRR19973072.2438724/150‑1 (MQ=60)
                                                                                                                                            GAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAC           <  SRR19973072.768780/150‑1 (MQ=60)
                                                                                                                                             AATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACC          >  SRR19973072.1333430/1‑150 (MQ=60)
                                                                                                                                               TCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCAC        <  SRR19973072.3290286/150‑1 (MQ=60)
                                                                                                                                               TCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCAC        <  SRR19973072.3289983/150‑1 (MQ=60)
                                                                                                                                                 CCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT      >  SRR19973072.2471264/1‑150 (MQ=60)
                                                                                                                                                    TAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAA   >  SRR19973072.233544/1‑150 (MQ=60)
                                                                                                                                                    TAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAA   >  SRR19973072.3398175/1‑150 (MQ=60)
                                                                                                                                                     AACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGATCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  SRR19973072.1563163/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  CP009273/3224778‑3225076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑