Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,224,927 C→A 100% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,224,9270CA100.0% 628.9 / ‑6.7 203intergenic (+329/‑97)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (82/120);  total (82/121)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224783‑3225071
                                                                                                                                                |                                                                                                                                                
tCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTGATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATaaca                                                                                                                                               <  2:487257/148‑1 (MQ=255)
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              <  1:2538898/148‑1 (MQ=255)
 cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACaa                                                                                                                                              >  2:454013/1‑148 (MQ=255)
   cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAc                                                                                                                                            <  1:123386/148‑1 (MQ=255)
   cATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAc                                                                                                                                            >  2:1123617/1‑148 (MQ=255)
     tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATATCAAAccc                                                                                                                                          >  2:2274585/1‑148 (MQ=255)
     tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAccc                                                                                                                                          >  2:2740442/1‑148 (MQ=255)
      ccGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcccc                                                                                                                                         >  2:1729798/1‑148 (MQ=255)
          cATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCAc                                                                                                                                     >  2:2931332/1‑148 (MQ=255)
            tCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTc                                                                                                                                   <  1:1336805/148‑1 (MQ=255)
            tCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTc                                                                                                                                   >  2:1292690/1‑148 (MQ=255)
            tCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAAAACAAACCCCGCACTc                                                                                                                                   >  1:2087120/1‑148 (MQ=255)
               gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcccc                                                                                                                                <  1:447083/148‑1 (MQ=255)
                cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCccct                                                                                                                               >  2:1524983/1‑148 (MQ=255)
                  ccGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttt                                                                                                                             <  1:804993/148‑1 (MQ=255)
                     cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcc                                                                                                                          >  1:1341305/1‑148 (MQ=255)
                     cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcc                                                                                                                          >  1:1867971/1‑148 (MQ=255)
                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  2:1040117/148‑1 (MQ=255)
                        cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCGGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                       <  1:1702175/148‑1 (MQ=255)
                         tcTGGCGGATGCGGCGTGTACGCCTTATCCGCCCTACATGTGCAATTCCGTACCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcccttt                                                                                                                      <  2:615798/147‑1 (MQ=255)
                          cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  2:2242670/148‑1 (MQ=255)
                          cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     >  1:1075909/1‑148 (MQ=255)
                          cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  2:1013843/148‑1 (MQ=255)
                           tGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCccttttcccttttc                                                                                                                    <  1:2585444/148‑1 (MQ=255)
                             gCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                  <  1:1327546/148‑1 (MQ=255)
                                gATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCgg                                                                                                               <  1:1769259/148‑1 (MQ=255)
                                     ggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACg                                                                                                          <  2:928115/148‑1 (MQ=255)
                                      gcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACgg                                                                                                         <  1:1860176/148‑1 (MQ=255)
                                      gcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACgg                                                                                                         <  1:29938/148‑1 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  1:3497978/148‑1 (MQ=255)
                                          ggACGCCTTATCCGCCCTACATGTGCAATGCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  1:3498155/148‑1 (MQ=255)
                                            aCGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAt                                                                                                   <  1:427270/148‑1 (MQ=255)
                                                  tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAc                                                                                             <  2:2641617/148‑1 (MQ=255)
                                                     ccGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCa                                                                                          >  1:60843/1‑148 (MQ=255)
                                                      cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  1:1295542/148‑1 (MQ=255)
                                                      cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:1229843/148‑1 (MQ=255)
                                                      cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:3066798/148‑1 (MQ=255)
                                                       gCCCTACATGTGCAAGTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATc                                                                                        <  1:287146/148‑1 (MQ=255)
                                                        cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATcc                                                                                       >  1:3158559/1‑148 (MQ=255)
                                                         ccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCGCCGGCGACGGCTAAATTAGAACTCATCCg                                                                                      <  2:1902394/148‑1 (MQ=255)
                                                              aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcac                                                                                 <  1:1672334/148‑1 (MQ=255)
                                                              aTGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcac                                                                                 <  1:731152/148‑1 (MQ=255)
                                                               tgtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcaca                                                                                <  1:1576361/148‑1 (MQ=255)
                                                               tgtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcaca                                                                                >  2:1584899/1‑148 (MQ=255)
                                                                 tgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATa                                                                              <  2:1403977/148‑1 (MQ=255)
                                                                  gCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATaa                                                                             <  1:2050631/148‑1 (MQ=255)
                                                                    aaTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACa                                                                           <  2:3424291/148‑1 (MQ=255)
                                                                     aTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACaa                                                                          <  1:2957958/148‑1 (MQ=255)
                                                                      ttCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAat                                                                         >  2:2190288/1‑148 (MQ=255)
                                                                       tCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAatt                                                                        <  2:786950/148‑1 (MQ=255)
                                                                         cGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattat                                                                      <  1:3013465/148‑1 (MQ=255)
                                                                         cGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAACACATAACAattat                                                                      <  1:3460892/148‑1 (MQ=255)
                                                                          gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  2:2141489/148‑1 (MQ=255)
                                                                          gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  2:2324677/148‑1 (MQ=255)
                                                                          gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  1:2403160/148‑1 (MQ=255)
                                                                          gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  2:1514265/148‑1 (MQ=255)
                                                                          gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     >  1:1980816/1‑148 (MQ=255)
                                                                              ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                 <  1:298067/148‑1 (MQ=255)
                                                                              ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                 <  2:60843/148‑1 (MQ=255)
                                                                               cTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACACATCCGACCACATAACAATTATTTTaaa                                                                >  1:653969/1‑146 (MQ=255)
                                                                               cTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACa                                                                <  2:396231/148‑1 (MQ=255)
                                                                                tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATGACAATTATTTTACAt                                                               >  2:3457991/1‑148 (MQ=255)
                                                                                tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATGACAATTATTTTACAt                                                               >  2:2109084/1‑148 (MQ=255)
                                                                                tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAt                                                               <  1:3188045/148‑1 (MQ=255)
                                                                                tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAAATATTTTACAt                                                               >  1:2257642/1‑148 (MQ=255)
                                                                                  gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGAGTATTCTTTTTGAAACCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCGAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAc                                                             >  2:1907938/1‑148 (MQ=255)
                                                                                     aaaaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                          <  1:482173/148‑1 (MQ=255)
                                                                                       aaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCACACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAc                                                        <  1:1406996/148‑1 (MQ=255)
                                                                                        aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCTCAGTCCGCTTTTCCCTTTTCTCCGTCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAGACTGGACa                                                       <  2:3367040/148‑1 (MQ=255)
                                                                                        aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACa                                                       <  2:3279393/148‑1 (MQ=255)
                                                                                        aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTACCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACa                                                       <  1:702368/148‑1 (MQ=255)
                                                                                         cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACaa                                                      >  1:1554557/1‑148 (MQ=255)
                                                                                          gcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAt                                                     <  2:3086330/148‑1 (MQ=255)
                                                                                              cAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTTAATCCCATAACAAACCCCGCACTCCCCGTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTa                                                 >  1:669993/1‑148 (MQ=255)
                                                                                              cAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACGCATCCGACCACATAACCATTATTTTACCTACAGGCCAATTTTa                                                 >  2:2979032/1‑148 (MQ=255)
                                                                                                aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  2:202207/148‑1 (MQ=255)
                                                                                                aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  1:1172522/148‑1 (MQ=255)
                                                                                                aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCGTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               >  1:1418984/1‑148 (MQ=255)
                                                                                                       cATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTAcc                                        >  1:446580/1‑148 (MQ=255)
                                                                                                         tCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCg                                      >  2:2566452/1‑148 (MQ=255)
                                                                                                          ccGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGt                                     <  1:2740442/148‑1 (MQ=255)
                                                                                                            ggCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCg                                   <  2:1840653/148‑1 (MQ=255)
                                                                                                                   tCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCg                            >  2:3364423/1‑148 (MQ=255)
                                                                                                                     aCCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTACGGCGACGGCGAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGGTCGcc                          >  2:696388/1‑148 (MQ=255)
                                                                                                                         ggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGc                      >  2:3410694/1‑148 (MQ=255)
                                                                                                                         ggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGc                      <  1:2612380/148‑1 (MQ=255)
                                                                                                                             tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  <  1:2355638/148‑1 (MQ=255)
                                                                                                                             tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  <  2:652839/148‑1 (MQ=255)
                                                                                                                             tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  >  2:2976661/1‑148 (MQ=255)
                                                                                                                             tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  <  2:2341479/148‑1 (MQ=255)
                                                                                                                               ttCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAtt                <  2:240441/148‑1 (MQ=255)
                                                                                                                               ttCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAtt                <  2:1341305/148‑1 (MQ=255)
                                                                                                                               ttCTTTTTGAAGCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAtt                <  2:1865322/148‑1 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               >  2:2465760/1‑148 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               <  1:173744/148‑1 (MQ=255)
                                                                                                                                tCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAttt               <  1:1125089/148‑1 (MQ=255)
                                                                                                                                   ttttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAgg            >  2:2440705/1‑148 (MQ=255)
                                                                                                                                       gAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGtttt        <  1:253313/148‑1 (MQ=255)
                                                                                                                                        aaTCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTa       >  2:2043650/1‑148 (MQ=255)
                                                                                                                                          tCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAcc     >  1:1050374/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224783‑3225071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA  >  CP009273/3224778‑3225074
                                                                                                                                                            |                                                                                                                                                   
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGTATCCCATA                                                                                                                                                     >  SRR19973065.203764/1‑150 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                     >  SRR19973065.1988363/1‑150 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCCTAACAAAC                                                                                                                                               >  SRR19973065.2712270/1‑150 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAC                                                                                                                                               <  SRR19973065.2555228/150‑1 (MQ=60)
      CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAC                                                                                                                                               <  SRR19973065.2554814/150‑1 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCC                                                                                                                                             <  SRR19973065.1986304/150‑1 (MQ=60)
        CATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCC                                                                                                                                             <  SRR19973065.124459/150‑1 (MQ=60)
               CATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAAAACAAACCCCGCACTC                                                                                                                                      >  SRR19973065.2100507/1‑150 (MQ=60)
                 TCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCC                                                                                                                                    <  SRR19973065.1345659/150‑1 (MQ=60)
                 TCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCC                                                                                                                                    <  SRR19973065.1345605/150‑1 (MQ=60)
                   TGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCG                                                                                                                                  >  SRR19973065.2816992/1‑150 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                 <  SRR19973065.450174/150‑1 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                 <  SRR19973065.450183/150‑1 (MQ=60)
                       tgcacctggTGGCGGATGCGGCGTACACGCCTTATCCGCCCTACATGTGCAAT‑‑‑‑‑‑‑CCTCCTGGCCGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTC                                                                                                                              <  SRR19973065.9447/141‑1 (MQ=40)
                       CCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTC                                                                                                                              <  SRR19973065.810314/150‑1 (MQ=60)
                        CGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCC                                                                                                                             >  SRR19973065.1350183/1‑150 (MQ=60)
                        CGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCC                                                                                                                             >  SRR19973065.1880266/1‑150 (MQ=60)
                        CGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTCC                                                                                                                             >  SRR19973065.1349879/1‑150 (MQ=60)
                        CGCGCCACTGGCGGATGCGGCGGGGACGCCTTATCCGCCCTACATGTGCAATTACGTAACCT‑‑‑‑‑‑‑GGAAAAAACGCTACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATACCATAACAAACACCGCCCGCCCCTTTTCA                                                                                                                             >  SRR19973065.1877599/1‑150 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                          >  SRR19973065.3445193/1‑150 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                          <  SRR19973065.465574/150‑1 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                        <  SRR19973065.1713355/150‑1 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCGTTTCCCTTTT                                                                                                                        >  SRR19973065.1083022/1‑150 (MQ=60)
                             CACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCG‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTT                                                                                                                        <  SRR19973065.1713516/150‑1 (MQ=60)
                                TGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTC                                                                                                                     <  SRR19973065.2601548/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGTACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                   <  SRR19973065.1336231/150‑1 (MQ=60)
                                  GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCG                                                                                                                   <  SRR19973065.1336325/150‑1 (MQ=60)
                                     GATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCG                                                                                                                <  SRR19973065.1780853/150‑1 (MQ=60)
                                           GCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCT                                                                                                          <  SRR19973065.1872414/150‑1 (MQ=60)
                                           GCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCT                                                                                                          <  SRR19973065.30321/150‑1 (MQ=60)
                                           GCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCT                                                                                                          <  SRR19973065.1870044/150‑1 (MQ=60)
                                            CGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTA                                                                                                         <  SRR19973065.3509046/150‑1 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                      <  SRR19973065.3519230/150‑1 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                      <  SRR19973065.3519347/150‑1 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAAACCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGCCGGCTAAAT                                                                                                      >  SRR19973065.2465197/1‑150 (MQ=60)
                                               GGACGCCTTATCCGCCCTACATGTGCAATGCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAT                                                                                                      <  SRR19973065.3519409/150‑1 (MQ=60)
                                                 ACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTA                                                                                                    <  SRR19973065.430241/150‑1 (MQ=60)
                                                 ACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTA                                                                                                    <  SRR19973065.430352/150‑1 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTCTTCTTTTTTAATCCCAAAACAAACCCCGCACTCCCCGTTTCCCTTTTATCCGGCGACGGCTAAATTAGAAA                                                                                                >  SRR19973065.9794/1‑150 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAAC                                                                                                >  SRR19973065.2996589/1‑150 (MQ=60)
                                                      TTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACT                                                                                               <  SRR19973065.1277776/150‑1 (MQ=60)
                                                       TATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCGAAATTAGAACTC                                                                                              >  SRR19973065.1392003/1‑150 (MQ=60)
                                                        ATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATTTCCCTTTTCGCCGGCGACGGCTAAATTAGAACTCA                                                                                             >  SRR19973065.61489/1‑150 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATNC                                                                                          <  SRR19973065.1304077/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                          >  SRR19973065.790124/1‑150 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                          >  SRR19973065.3177705/1‑150 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                          >  SRR19973065.1774381/1‑150 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCG‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                          <  SRR19973065.2612068/150‑1 (MQ=60)
                                                            GCCCTACATGTGCAAGTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCG                                                                                         <  SRR19973065.289030/150‑1 (MQ=60)
                                                                   ATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAT                                                                                  <  SRR19973065.1683520/150‑1 (MQ=60)
                                                                   ATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAT                                                                                  <  SRR19973065.1683517/150‑1 (MQ=60)
                                                                   ATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAT                                                                                  <  SRR19973065.736048/150‑1 (MQ=60)
                                                                    TGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATA                                                                                 <  SRR19973065.1586862/150‑1 (MQ=60)
                                                                     GTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATGCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAC                                                                                >  SRR19973065.40114/1‑150 (MQ=60)
                                                                     GTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAA                                                                                >  SRR19973065.657140/1‑150 (MQ=60)
                                                                     GTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTCAATTAGAACTCATCCGACCACATAA                                                                                >  SRR19973065.1569888/1‑150 (MQ=60)
                                                                       GCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                              <  SRR19973065.2063731/150‑1 (MQ=60)
                                                                       GCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                              <  SRR19973065.2063727/150‑1 (MQ=60)
                                                                       GCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCGTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACA                                                                              >  SRR19973065.2109791/1‑150 (MQ=60)
                                                                          ATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATT                                                                           <  SRR19973065.2976016/150‑1 (MQ=60)
                                                                             CCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATT                                                                        >  SRR19973065.1993697/1‑150 (MQ=60)
                                                                              CGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                       <  SRR19973065.3031920/150‑1 (MQ=60)
                                                                              CGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTT                                                                       <  SRR19973065.3031919/150‑1 (MQ=60)
                                                                              CGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAACACATAACAATTATGT                                                                       <  SRR19973065.3481977/150‑1 (MQ=60)
                                                                               GTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                      <  SRR19973065.2418272/150‑1 (MQ=60)
                                                                               GTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                      <  SRR19973065.2418262/150‑1 (MQ=60)
                                                                                  ACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACACATCCGACCACATAACAATTATTTTAAA                                                                   >  SRR19973065.658280/1‑150 (MQ=60)
                                                                                   CCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAT                                                                  <  SRR19973065.300109/150‑1 (MQ=60)
                                                                                   CCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAT                                                                  <  SRR19973065.299991/150‑1 (MQ=60)
                                                                                   CCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAAATATTTTACAT                                                                  >  SRR19973065.2271929/1‑150 (MQ=60)
                                                                                     T‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                <  SRR19973065.3207283/150‑1 (MQ=60)
                                                                                     T‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAC                                                                <  SRR19973065.3204166/150‑1 (MQ=60)
                                                                                                 AAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAC                                                           <  SRR19973065.485454/150‑1 (MQ=60)
                                                                                                   AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAA                                                         >  SRR19973065.1564863/1‑150 (MQ=60)
                                                                                                   AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACGCCCCTTTTACCTTTTCTCCGGCGACGGCTAAATTAGAACTCAACCGACCACATCACAATTATTTTACATACTGGACAA                                                         >  SRR19973065.3531673/1‑150 (MQ=60)
                                                                                                   AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCACACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAA                                                         <  SRR19973065.1416209/150‑1 (MQ=60)
                                                                                                   AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCACACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAA                                                         <  SRR19973065.697962/150‑1 (MQ=60)
                                                                                                    ACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAT                                                        <  SRR19973065.707121/150‑1 (MQ=60)
                                                                                                    ACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTACCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAT                                                        <  SRR19973065.707039/150‑1 (MQ=60)
                                                                                                     CGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACATACTGGACAATT                                                       >  SRR19973065.3168177/1‑150 (MQ=60)
                                                                                                      GCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTT                                                      <  SRR19973065.2321294/150‑1 (MQ=60)
                                                                                                        GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTTAATCCCATAACAAACCCCGCACTCCCCGTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAAAAATTATTTTACATACTGGACAATTTTA                                                    >  SRR19973065.674458/1‑150 (MQ=60)
                                                                                                        GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGTATCCCATAACAAACCCCCCCCTCCCCATTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACATACTGGACAATTTTA                                                    >  SRR19973065.674426/1‑150 (MQ=60)
                                                                                                        GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATGCCATAACAAACCCCGCACTCCCCGTTTCCCGTTTCTCCGGCGACGGCGCAATTAGAACTCAACCGACCACCGAACAATTATTTTACATACTGGACAATTTTA                                                    >  SRR19973065.1203884/1‑150 (MQ=60)
                                                                                                          CAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCGTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATG                                                  >  SRR19973065.1428245/1‑150 (MQ=60)
                                                                                                            AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                                <  SRR19973065.1179992/150‑1 (MQ=60)
                                                                                                                 CGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACC                                           >  SRR19973065.449676/1‑150 (MQ=60)
                                                                                                                      CCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCG                                      <  SRR19973065.2757488/150‑1 (MQ=60)
                                                                                                                        GGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCT                                    <  SRR19973065.2949119/150‑1 (MQ=60)
                                                                                                                           GTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATGCTGGACAATTTTATGAGCTACCCGTCGCTGTT                                 >  SRR19973065.1075163/1‑150 (MQ=60)
                                                                                                                           GTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAAATAATGGACAATTTTATTAGCTACCCGTCGCTGTT                                 >  SRR19973065.111727/1‑150 (MQ=60)
                                                                                                                                 ACCGGGCGTATTCTTTTTGAATCCCATAACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCC                           <  SRR19973065.1130936/150‑1 (MQ=60)
                                                                                                                                     GGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTG                       <  SRR19973065.2628574/150‑1 (MQ=60)
                                                                                                                                        GTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAACTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAT                    >  SRR19973065.2085923/1‑150 (MQ=60)
                                                                                                                                         TATTCTTTTTGAATCCCATAACACACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATT                   <  SRR19973065.2013621/150‑1 (MQ=60)
                                                                                                                                         TATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATT                   <  SRR19973065.1741257/150‑1 (MQ=60)
                                                                                                                                         TATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATT                   <  SRR19973065.2370284/150‑1 (MQ=60)
                                                                                                                                         TATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATT                   <  SRR19973065.580875/150‑1 (MQ=60)
                                                                                                                                            TCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAG                <  SRR19973065.175130/150‑1 (MQ=60)
                                                                                                                                            TCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAG                <  SRR19973065.1132412/150‑1 (MQ=60)
                                                                                                                                                   GAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAC         <  SRR19973065.255085/150‑1 (MQ=60)
                                                                                                                                                   GAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTCC         <  SRR19973065.255663/150‑1 (MQ=60)
                                                                                                                                                    AATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACC        <  SRR19973065.144624/150‑1 (MQ=60)
                                                                                                                                                    AATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACC        >  SRR19973065.1057330/1‑150 (MQ=60)
                                                                                                                                                      TCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCAC      >  SRR19973065.1158213/1‑150 (MQ=60)
                                                                                                                                                       CCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG     >  SRR19973065.142534/1‑150 (MQ=60)
                                                                                                                                                         CATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTT   >  SRR19973065.1345441/1‑150 (MQ=60)
                                                                                                                                                          ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA  >  SRR19973065.2894405/1‑150 (MQ=60)
                                                                                                                                                          ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA  >  SRR19973065.2465489/1‑150 (MQ=60)
                                                                                                                                                            |                                                                                                                                                   
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCT‑‑‑‑‑‑‑GGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA  >  CP009273/3224778‑3225074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑