Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,224,927 C→A 100% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,224,9270CA100.0% 514.7 / NA 158intergenic (+329/‑97)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (58/100);  total (58/100)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 158 total aligned reads displayed.

CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224784‑3225071
                                                                                                                                               |                                                                                                                                                
cGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATGCCGTAACCTGGATAAAACGCGACACGCGTCGCATCCGGCGTTCTCACCGGGCGTCTTCTTTTTGAATCCCAGAACaa                                                                                                                                              >  2:3156215/1‑148 (MQ=21)
   aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                           <  1:549408/148‑1 (MQ=255)
   aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                           <  1:2425906/148‑1 (MQ=255)
   aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcc                                                                                                                                           <  1:2425845/148‑1 (MQ=255)
     ccGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAAcccc                                                                                                                                         >  2:1364216/1‑148 (MQ=255)
      cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCg                                                                                                                                        >  2:2899990/1‑148 (MQ=255)
        gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCa                                                                                                                                      <  1:571332/148‑1 (MQ=255)
        gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCa                                                                                                                                      <  2:1403509/148‑1 (MQ=255)
        gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCa                                                                                                                                      <  1:2234632/148‑1 (MQ=255)
            cTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcc                                                                                                                                  <  1:1011739/148‑1 (MQ=255)
            cTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcc                                                                                                                                  <  1:1011692/148‑1 (MQ=255)
            cTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATACCATAACAAACCCCGCACTcc                                                                                                                                  >  1:1691094/1‑148 (MQ=255)
              gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcccc                                                                                                                                >  1:1937456/1‑148 (MQ=255)
              gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTcccc                                                                                                                                <  2:767145/148‑1 (MQ=255)
              gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGGTATCACCGGGCGTATTCTTTTTGTATCCCATAACAAAACCCGCCCTcccc                                                                                                                                >  1:658154/1‑148 (MQ=25)
                 ccGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttt                                                                                                                             <  2:2642051/148‑1 (MQ=255)
                  cgcgCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                            <  2:1270421/148‑1 (MQ=255)
                  cgcgCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                            <  2:3050598/148‑1 (MQ=255)
                  cgcgCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                            >  2:759901/1‑148 (MQ=255)
                  cgcgCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCccctttt                                                                                                                            <  2:1660765/148‑1 (MQ=255)
                    cgcgACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcc                                                                                                                          <  2:2046059/148‑1 (MQ=255)
                    cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttcc                                                                                                                          <  1:485803/148‑1 (MQ=255)
                    cgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccatttac                                                                                                                          >  1:61273/1‑146 (MQ=255)
                     gcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccc                                                                                                                         <  1:499049/148‑1 (MQ=255)
                      ccACTGGCGGATGCGGCGTGGACGCCTTATCTGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  2:699300/148‑1 (MQ=255)
                      ccACTGGCGGATGCGGCGTGGACGCCTTATCTGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccct                                                                                                                        <  2:1463051/148‑1 (MQ=255)
                       cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                       >  1:1395211/1‑148 (MQ=255)
                       cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctt                                                                                                                       <  2:1593082/148‑1 (MQ=255)
                         cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  1:3062041/148‑1 (MQ=255)
                         cTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCcccttttccctttt                                                                                                                     <  1:2754366/148‑1 (MQ=255)
                               gATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCgg                                                                                                               <  2:2762276/148‑1 (MQ=255)
                                aTGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc                                                                                                              <  1:1288153/148‑1 (MQ=255)
                                  gcggcgTGGACGCCTTATCCGCCCTCCATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGa                                                                                                            <  1:515963/148‑1 (MQ=255)
                                   cggcgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCgcc                                                                                                           >  2:1028942/1‑146 (MQ=255)
                                      cgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                        <  2:3007408/148‑1 (MQ=255)
                                      cgTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                        <  2:1290459/148‑1 (MQ=255)
                                        tGGACGCCTTATCCGCCCTACATGTGCAAGTCCGTACCCTGGATAAAACGCGACACGCGTCGCAACCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTa                                                                                                      <  2:272026/148‑1 (MQ=38)
                                         ggACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTaa                                                                                                     <  2:348395/148‑1 (MQ=255)
                                                ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                              >  1:1671267/1‑148 (MQ=255)
                                                ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGaa                                                                                              >  1:3311740/1‑148 (MQ=255)
                                                 tATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGCCGGCTAAATTAGAAc                                                                                             >  1:2069956/1‑148 (MQ=255)
                                                   tCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                           <  2:2572753/148‑1 (MQ=255)
                                                   tCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTc                                                                                           <  1:219655/148‑1 (MQ=255)
                                                     cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:3201765/148‑1 (MQ=255)
                                                     cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:3201762/148‑1 (MQ=255)
                                                     cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:3149407/148‑1 (MQ=255)
                                                     cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:1019279/148‑1 (MQ=255)
                                                     cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                         <  2:1004221/148‑1 (MQ=255)
                                                          tACATGTGCAATTCCGAAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTCTTCTTTTTGAAACCCATAACAAACCCCGCACTCCCCTTTTCCCATTTCTCCGGCGACGGCTAAATTAGAACTCAACCGAc                                                                                    >  2:1120500/1‑148 (MQ=255)
                                                             aTGTGCAAGTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcac                                                                                 <  2:2920725/148‑1 (MQ=255)
                                                                 gCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATaa                                                                             >  2:466653/1‑148 (MQ=255)
                                                                    aTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACaa                                                                          <  1:2705471/148‑1 (MQ=255)
                                                                     ttCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCACCCGACCCCAGAACAat                                                                         >  2:3232515/1‑148 (MQ=255)
                                                                       ccGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTGTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAatta                                                                       >  2:2623132/1‑148 (MQ=255)
                                                                        cGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattat                                                                      <  2:2188306/148‑1 (MQ=255)
                                                                         gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  2:453065/148‑1 (MQ=255)
                                                                         gTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAattatt                                                                     <  1:1578477/148‑1 (MQ=255)
                                                                          tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAttt                                                                    <  2:1828441/148‑1 (MQ=255)
                                                                          tAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAttt                                                                    <  2:672269/148‑1 (MQ=255)
                                                                           aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAtttt                                                                   <  2:1671267/148‑1 (MQ=255)
                                                                             ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                 >  1:1339106/1‑148 (MQ=255)
                                                                             ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAc                                                                 <  1:2028973/148‑1 (MQ=255)
                                                                               tGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAt                                                               >  1:1670382/1‑148 (MQ=255)
                                                                                 gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAc                                                             <  1:1364216/148‑1 (MQ=255)
                                                                                  aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACt                                                            <  1:2708233/148‑1 (MQ=255)
                                                                                  aTAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACt                                                            >  2:889689/1‑148 (MQ=255)
                                                                                   tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATAccg                                                           <  1:2870899/148‑3 (MQ=255)
                                                                                   tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTg                                                           <  1:2870895/148‑1 (MQ=255)
                                                                                   tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAGAACAATTATTTTACATACTg                                                           >  2:1407068/1‑148 (MQ=255)
                                                                                    aataCGCGACAAGCGTCGCATCCGGCTTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGAAGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                          <  2:2040575/145‑1 (MQ=255)
                                                                                    aataCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                          <  1:3101321/145‑1 (MQ=255)
                                                                                    aaaaCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTgg                                                          <  1:3101131/148‑1 (MQ=255)
                                                                                       aCGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACa                                                       >  2:1000304/1‑148 (MQ=255)
                                                                                        cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACaa                                                      <  1:950258/148‑1 (MQ=255)
                                                                                        cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAAAACAATTATTTTACATACTGGACaa                                                      >  1:926640/1‑148 (MQ=255)
                                                                                          cgACCAGCGTCGCATCCGTCGTTATCACCGGGCGTATTCTTTTTGAATCCCACAACAAACCCCGCACTACCATTGTCCCTTTTCGCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACATTTATTTTACATACTGGACAAtt                                                    <  2:3275852/148‑1 (MQ=255)
                                                                                           gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAAttt                                                   <  1:1270664/148‑1 (MQ=255)
                                                                                              aaGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCCCTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTAt                                                >  2:338334/1‑148 (MQ=255)
                                                                                               aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  1:2807712/148‑1 (MQ=255)
                                                                                               aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  1:3144536/148‑1 (MQ=255)
                                                                                               aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  1:3144495/148‑1 (MQ=255)
                                                                                               aGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                               <  1:2807713/148‑1 (MQ=255)
                                                                                                    cGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCCCATAACACTTATTTTACATACTGGACAATTTTATGAGCTa                                          <  1:200259/148‑1 (MQ=255)
                                                                                                          cGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTc                                    >  2:515065/1‑148 (MQ=255)
                                                                                                           ggCGTTATCACCGGGCGTATTCTTTTTGCATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCg                                   <  2:1660076/148‑1 (MQ=255)
                                                                                                                tATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGtt                              <  1:759901/148‑1 (MQ=255)
                                                                                                                    aCCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGcc                          <  2:1462139/148‑1 (MQ=255)
                                                                                                                       gggCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCg                       <  1:1504713/148‑1 (MQ=255)
                                                                                                                            tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  >  1:819547/1‑148 (MQ=255)
                                                                                                                            tATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTGACATACTGGACAAATTTATGAGCTACCCGTCGCTTGTAGCCCCGcagta                  >  1:2477742/1‑144 (MQ=255)
                                                                                                                            tATTCATTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGa                  <  2:1770351/148‑1 (MQ=255)
                                                                                                                             aTTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                 >  1:1563437/1‑148 (MQ=255)
                                                                                                                             aTTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                 >  1:1564029/1‑148 (MQ=255)
                                                                                                                             aTTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAt                 >  2:1615709/1‑148 (MQ=255)
                                                                                                                                    ttGAATCCCATACCAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGtt          <  1:2628261/148‑1 (MQ=255)
                                                                                                                                    ttGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGTCGACGGCTAAATTAGAACTCAACCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGtt          <  1:2628292/148‑1 (MQ=255)
                                                                                                                                       aaTCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTAGCCCCGCTGGATTTAGGTTTTa       >  2:2227687/1‑148 (MQ=255)
                                                                                                                                        aTCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAc      >  2:2088958/1‑148 (MQ=255)
                                                                                                                                           ccATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCAc   >  2:3272201/1‑148 (MQ=255)
                                                                                                                                            cATAACACACCCCGCACTCCCCAGTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATACCAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACg  <  2:3042565/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACG  >  CP009273/3224784‑3225071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  CP009273/3224778‑3225076
                                                                                                                                                     |                                                                                                                                                     
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGTATCCCATA                                                                                                                                                       >  SRR19973070.1299456/1‑150 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       >  SRR19973070.2958151/1‑150 (MQ=60)
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATA                                                                                                                                                       <  SRR19973070.2045223/150‑1 (MQ=60)
  GCGTAGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGCACGCCTTATCCGCCCTACATGTGCTTTTCCGTACCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAAC                                                                                                                                                     <  SRR19973070.2486563/150‑1 (MQ=60)
         ATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCC                                                                                                                                              <  SRR19973070.2441849/150‑1 (MQ=60)
         ATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCC                                                                                                                                              <  SRR19973070.2441848/150‑1 (MQ=60)
         ATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCC                                                                                                                                              <  SRR19973070.2441788/150‑1 (MQ=60)
         ATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCC                                                                                                                                              <  SRR19973070.553061/150‑1 (MQ=60)
              GCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACT                                                                                                                                         <  SRR19973070.2249366/150‑1 (MQ=60)
              GCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACT                                                                                                                                         <  SRR19973070.2249364/150‑1 (MQ=60)
              GCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACT                                                                                                                                         <  SRR19973070.575169/150‑1 (MQ=60)
              GCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACT                                                                                                                                         <  SRR19973070.575217/150‑1 (MQ=60)
                ATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATACCATAACAAACCCCGCACTCC                                                                                                                                       >  SRR19973070.1702717/1‑150 (MQ=60)
                 TCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCC                                                                                                                                      <  SRR19973070.100241/150‑1 (MQ=60)
                  CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCC                                                                                                                                     <  SRR19973070.1018884/150‑1 (MQ=60)
                  CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCC                                                                                                                                     >  SRR19973070.1950481/1‑150 (MQ=60)
                  CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCC                                                                                                                                     <  SRR19973070.1018931/150‑1 (MQ=60)
                  CTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGGTATCACCGGGCGTATTCTTTTTGTATCCCATAACAAAACCCGCCCTCCCC                                                                                                                                     >  SRR19973070.662843/1‑150 (MQ=60)
                    GCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTT                                                                                                                                   <  SRR19973070.696588/150‑1 (MQ=60)
                     CACCGCGCCACTGGCGGATGCGGCGTGGACGCCGTATACGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGGATTATTTTTGTATACCATAACAAACCCCccccaccccatt                                                                                                                                  >  SRR19973070.284718/1‑138 (MQ=60)
                      ACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTT                                                                                                                                 <  SRR19973070.3050908/150‑1 (MQ=60)
                        CGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCATTTAC                                                                                                                               >  SRR19973070.61968/1‑150 (MQ=60)
                          CGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCT                                                                                                                             <  SRR19973070.489223/150‑1 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            >  SRR19973070.1404613/1‑150 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            <  SRR19973070.502522/150‑1 (MQ=60)
                           GCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTT                                                                                                                            >  SRR19973070.1404616/1‑150 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                        <  SRR19973070.2772051/150‑1 (MQ=60)
                               CTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCT                                                                                                                        <  SRR19973070.3081647/150‑1 (MQ=60)
                                 GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCCTAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCC                                                                                                                      >  SRR19973070.3102202/1‑150 (MQ=60)
                                    GGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC                                                                                                                   <  SRR19973070.1408153/150‑1 (MQ=60)
                                    GGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC                                                                                                                   <  SRR19973070.1950220/150‑1 (MQ=60)
                                      ATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGA                                                                                                                 <  SRR19973070.1297136/150‑1 (MQ=60)
                                        GCGGCGTGGACGCCTTATCCGCCCTCCATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACG                                                                                                               <  SRR19973070.519495/150‑1 (MQ=60)
                                        GCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACG                                                                                                               <  SRR19973070.519406/150‑1 (MQ=60)
                                                  CGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG                                                                                                     >  SRR19973070.211650/1‑150 (MQ=60)
                                                    CCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAA                                                                                                   >  SRR19973070.3332920/1‑150 (MQ=60)
                                                    CCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAA                                                                                                   >  SRR19973070.1682730/1‑150 (MQ=60)
                                                     CTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGCCGGCTAAATTAGAAC                                                                                                  >  SRR19973070.2083900/1‑150 (MQ=60)
                                                         TCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAT                                                                                              <  SRR19973070.221247/150‑1 (MQ=60)
                                                           CGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCC                                                                                            <  SRR19973070.659959/150‑1 (MQ=60)
                                                              CCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                         <  SRR19973070.13178/150‑1 (MQ=60)
                                                              CCTACATGTGCAAGTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGAC                                                                                         <  SRR19973070.1701300/150‑1 (MQ=60)
                                                                          ATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATT                                                                             <  SRR19973070.2722964/150‑1 (MQ=60)
                                                                            TCCGTATCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTAT                                                                           <  SRR19973070.2644434/150‑1 (MQ=60)
                                                                               GTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTT                                                                        <  SRR19973070.1589548/150‑1 (MQ=60)
                                                                                 AACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTAC                                                                      >  SRR19973070.1348314/1‑150 (MQ=60)
                                                                                   CCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAT                                                                    <  SRR19973070.2042691/150‑1 (MQ=60)
                                                                                   CCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAT                                                                    >  SRR19973070.1681839/1‑150 (MQ=60)
                                                                                   CCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACAT                                                                    >  SRR19973070.1681844/1‑150 (MQ=60)
                                                                                       GATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTG                                                                <  SRR19973070.1373514/150‑1 (MQ=60)
                                                                                        ATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGG                                                               <  SRR19973070.2725740/150‑1 (MQ=60)
                                                                                         TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTGTTTTTTTATCCCATAACAAACCCCGCACGCCCCTTTTCCCTTTTATCCGGCGACGGCGAAATTAGAACTCATACGACCACATAACAATTATTTTACATACTGGT                                                              >  SRR19973070.344005/1‑150 (MQ=60)
                                                                                         TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGA                                                              <  SRR19973070.2889492/150‑1 (MQ=60)
                                                                                         TAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACCGGA                                                              <  SRR19973070.2889496/150‑1 (MQ=60)
                                                                                          AATACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAC                                                             <  SRR19973070.3121316/150‑1 (MQ=60)
                                                                                          AAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGAC                                                             <  SRR19973070.3121126/150‑1 (MQ=60)
                                                                                            AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAA                                                           >  SRR19973070.933239/1‑150 (MQ=60)
                                                                                            AACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAAAACAATTATTTTACATACTGGACAA                                                           >  SRR19973070.933249/1‑150 (MQ=60)
                                                                                              CGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATT                                                         <  SRR19973070.957030/150‑1 (MQ=60)
                                                                                                 GACAATCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACATACCCCGGACTCCACTTTTCCCTTTTCTCAGGCGAAGGCTAAATTAGAACTCATCCGACCACATATCAATTATTTTACTTACTGGACAATTTTA                                                      <  SRR19973070.1278779/150‑1 (MQ=60)
                                                                                                 GACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTA                                                      <  SRR19973070.1279558/150‑1 (MQ=60)
                                                                                                     AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                                  <  SRR19973070.2825861/150‑1 (MQ=60)
                                                                                                     AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                                  <  SRR19973070.2825862/150‑1 (MQ=60)
                                                                                                     AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                                  <  SRR19973070.3164852/150‑1 (MQ=60)
                                                                                                     AGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAG                                                  <  SRR19973070.3164811/150‑1 (MQ=60)
                                                                                                          CGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCCCATAACACTTATTTTACATACTGGACAATTTTATGAGCTACC                                             <  SRR19973070.201779/150‑1 (MQ=60)
                                                                                                                    GTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTATAACTCATACGACCACATAACAATTATTTTAAATACTGGACAATTTTATGAGCTACCCGTCGCgggt                                   >  SRR19973070.3134214/1‑146 (MQ=60)
                                                                                                                    GTTATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCCCCCTGCCCGTTTCCCTTTTTTCCCGCGCCGGCGAAATTAGAACTCATCCCACCACCTAACCAATATTTTAACAACTGGACAAATTTATGAGCTACCCGTCGCGGTT                                   >  SRR19973070.3134219/1‑150 (MQ=55)
                                                                                                                      TATCACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCG                                 <  SRR19973070.765243/150‑1 (MQ=60)
                                                                                                                          ACCGGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCGGTTCGCCCC                             >  SRR19973070.2723635/1‑150 (MQ=60)
                                                                                                                             GGGCGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCT                          <  SRR19973070.1515243/150‑1 (MQ=60)
                                                                                                                                CGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGA                       >  SRR19973070.2808408/1‑150 (MQ=60)
                                                                                                                                CGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGA                       >  SRR19973070.825142/1‑150 (MQ=60)
                                                                                                                                CGTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTGACATACTGGACAAATTTATGAGCTACCCGTCGCTtgtagccccgcagta                       >  SRR19973070.2493880/1‑135 (MQ=60)
                                                                                                                                 GTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAT                      >  SRR19973070.1574439/1‑150 (MQ=60)
                                                                                                                                 GTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAT                      >  SRR19973070.1575037/1‑150 (MQ=60)
                                                                                                                                 GTATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGAT                      >  SRR19973070.1574441/1‑150 (MQ=60)
                                                                                                                                   ATTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTT                    >  SRR19973070.2646082/1‑150 (MQ=60)
                                                                                                                                    TTCTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTA                   >  SRR19973070.2034916/1‑150 (MQ=60)
                                                                                                                                      CTTTTTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGG                 >  SRR19973070.3298920/1‑150 (MQ=60)
                                                                                                                                          TTGAATCCCATACCAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTT             <  SRR19973070.2645407/150‑1 (MQ=60)
                                                                                                                                          TTGAATCCCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGTCGACGGCTAAATTAGAACTCAACCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTT             <  SRR19973070.2645438/150‑1 (MQ=60)
                                                                                                                                                 CCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTATGTTTTACCAAGT      >  SRR19973070.1145126/1‑150 (MQ=60)
                                                                                                                                                 CCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT      >  SRR19973070.2558941/1‑150 (MQ=60)
                                                                                                                                                 CCATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGT      >  SRR19973070.1145127/1‑150 (MQ=60)
                                                                                                                                                   ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTATGTTTTAACACGTTA    >  SRR19973070.1131013/1‑150 (MQ=60)
                                                                                                                                                   ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA    >  SRR19973070.2175344/1‑150 (MQ=60)
                                                                                                                                                   ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTA    <  SRR19973070.85037/150‑1 (MQ=60)
                                                                                                                                                   ATAACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCCCTGGATTTAGGTTTTAACACCTTA    >  SRR19973070.1130839/1‑150 (MQ=60)
                                                                                                                                                     AACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  SRR19973070.2991447/1‑150 (MQ=60)
                                                                                                                                                     AACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTAGCCCCGCGGGATTTAGGTTTTAACACGTTAAA  >  SRR19973070.1411390/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAA  >  CP009273/3224778‑3225076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑