Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,217,289 A→T 100% H433L (CAC→CTC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,217,2890AT99.6% 729.8 / ‑5.3 221H433L (CAC→CTC) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (105/115);  total (105/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA  >  CP009273/3217144‑3217433
                                                                                                                                                 |                                                                                                                                                
gAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcg                                                                                                                                                <  1:2782901/148‑1 (MQ=255)
    tgtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCa                                                                                                                                            >  1:1578295/1‑148 (MQ=255)
     gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAg                                                                                                                                           >  2:1722880/1‑148 (MQ=255)
       gtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                         <  1:3029139/148‑1 (MQ=255)
          atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaaaaa                                                                                                                                      >  2:1174212/1‑148 (MQ=255)
          atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaaaaa                                                                                                                                      >  2:3412216/1‑148 (MQ=255)
            atCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAcc                                                                                                                                    <  1:2669271/148‑1 (MQ=255)
               tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATc                                                                                                                                 >  1:3567546/1‑148 (MQ=255)
                aCGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATcc                                                                                                                                >  1:1285602/1‑148 (MQ=255)
                aCGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATcc                                                                                                                                >  1:1105920/1‑148 (MQ=255)
                aCGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATcc                                                                                                                                >  2:1322539/1‑148 (MQ=255)
                  ggCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGt                                                                                                                              >  1:2110669/1‑148 (MQ=255)
                   gCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGTATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTc                                                                                                                             >  1:2465862/1‑148 (MQ=255)
                            tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcat                                                                                                                    <  2:2757299/148‑1 (MQ=255)
                             gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                   <  2:882228/148‑1 (MQ=255)
                             gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                   <  1:3777922/148‑1 (MQ=255)
                               tGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTg                                                                                                                 <  2:1401016/148‑1 (MQ=255)
                                ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTgg                                                                                                                <  2:1563115/148‑1 (MQ=255)
                                 gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGt                                                                                                               >  1:174704/1‑148 (MQ=255)
                                   ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCg                                                                                                             <  2:750311/148‑1 (MQ=255)
                                     aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt                                                                                                           >  1:1759421/1‑148 (MQ=255)
                                       aCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCCGTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                         >  2:803979/1‑148 (MQ=255)
                                       aCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                         >  1:1465609/1‑148 (MQ=255)
                                           aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa                                                                                                     >  1:2604910/1‑148 (MQ=255)
                                           aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa                                                                                                     >  2:2545653/1‑148 (MQ=255)
                                               cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                 <  2:1998088/148‑1 (MQ=255)
                                               cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                 <  2:1994664/148‑1 (MQ=255)
                                                gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                <  1:571027/148‑1 (MQ=255)
                                                       aCGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTa                                                                                         <  1:1143457/148‑1 (MQ=255)
                                                        cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                        >  1:1820105/1‑148 (MQ=255)
                                                        cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                        >  1:1568964/1‑148 (MQ=255)
                                                           cTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGc                                                                                     >  1:35510/1‑148 (MQ=255)
                                                               gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTa                                                                                 <  1:2597143/148‑1 (MQ=255)
                                                                   aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                             <  2:487088/148‑1 (MQ=255)
                                                                     gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                                                                           >  2:1626494/1‑148 (MQ=255)
                                                                          cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcgcg                                                                      <  2:2659309/148‑1 (MQ=255)
                                                                             tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                   >  2:1943998/1‑148 (MQ=255)
                                                                              atTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGt                                                                  >  1:2022780/1‑148 (MQ=255)
                                                                               ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                 >  2:3555229/1‑148 (MQ=255)
                                                                               ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                 >  1:1706891/1‑148 (MQ=255)
                                                                               ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                 >  1:106768/1‑148 (MQ=255)
                                                                                tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAc                                                                >  2:3264880/1‑148 (MQ=255)
                                                                                tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAc                                                                >  1:430103/1‑148 (MQ=255)
                                                                                 aGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAcc                                                               >  1:313053/1‑148 (MQ=255)
                                                                                  gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                              >  1:1099487/1‑148 (MQ=255)
                                                                                  gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                              <  2:1374574/148‑1 (MQ=255)
                                                                                  gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                              >  1:413805/1‑148 (MQ=255)
                                                                                  gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                              <  1:2778676/148‑1 (MQ=255)
                                                                                    cGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATg                                                            <  2:2879660/148‑1 (MQ=255)
                                                                                    cGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATg                                                            <  2:35510/148‑1 (MQ=255)
                                                                                       aTTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcgg                                                         <  2:1050496/148‑1 (MQ=255)
                                                                                        ttACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggc                                                        >  2:1788702/1‑148 (MQ=255)
                                                                                         tACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggcg                                                       >  2:429680/1‑148 (MQ=255)
                                                                                         tACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggcg                                                       >  1:477700/1‑148 (MQ=255)
                                                                                            cgacgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaaa                                                    >  1:416749/1‑148 (MQ=255)
                                                                                            cgacgaGACGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaaa                                                    <  2:3674235/148‑1 (MQ=255)
                                                                                                gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcgc                                                <  2:3567546/148‑1 (MQ=255)
                                                                                                gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcgc                                                <  2:3688675/148‑1 (MQ=255)
                                                                                                gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcgc                                                <  2:701272/148‑1 (MQ=255)
                                                                                                 aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAATCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                               <  2:3706188/148‑1 (MQ=255)
                                                                                                 aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                               <  1:1997258/148‑1 (MQ=255)
                                                                                                   ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                             >  2:3015738/1‑148 (MQ=255)
                                                                                                    cGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGa                                            <  1:3455725/148‑1 (MQ=255)
                                                                                                    cGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGa                                            <  2:3313830/148‑1 (MQ=255)
                                                                                                     gCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGAt                                           <  2:1578065/148‑1 (MQ=255)
                                                                                                     gCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGAt                                           <  1:2539370/148‑1 (MQ=255)
                                                                                                      cAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATg                                          <  1:2391968/148‑1 (MQ=255)
                                                                                                       aGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGATGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGa                                         <  1:1332234/148‑1 (MQ=255)
                                                                                                         tGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGaca                                       >  1:283296/1‑148 (MQ=255)
                                                                                                          gggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGacac                                      >  1:2978085/1‑148 (MQ=255)
                                                                                                               aaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGAc                                 >  2:2775772/1‑148 (MQ=255)
                                                                                                                   tCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGt                             >  2:2580508/1‑148 (MQ=255)
                                                                                                                    cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                            >  1:750279/1‑148 (MQ=255)
                                                                                                                    cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                            >  1:750108/1‑148 (MQ=255)
                                                                                                                    cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                            <  1:674466/148‑1 (MQ=255)
                                                                                                                     tACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGc                           >  1:973731/1‑148 (MQ=255)
                                                                                                                      aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                          <  2:2733948/148‑1 (MQ=255)
                                                                                                                        gTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAtt                        <  1:504526/148‑1 (MQ=255)
                                                                                                                         tCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTa                       >  2:1538459/1‑148 (MQ=255)
                                                                                                                            aGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga                    >  2:2225342/1‑148 (MQ=255)
                                                                                                                               gcATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaaga                 <  2:1265686/148‑1 (MQ=255)
                                                                                                                               gcATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaaga                 <  2:3459589/148‑1 (MQ=255)
                                                                                                                               gcATTGTTCGCCAGATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaaga                 <  2:2142864/148‑1 (MQ=255)
                                                                                                                                cATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGACTGGTGCATTACgaagaa                >  2:244696/1‑148 (MQ=255)
                                                                                                                                 aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGaagaag               <  2:3111110/148‑1 (MQ=255)
                                                                                                                                  ttGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga              >  1:1877410/1‑148 (MQ=255)
                                                                                                                                  ttGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga              <  2:3277422/148‑1 (MQ=255)
                                                                                                                                   tGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt             <  1:2146878/148‑1 (MQ=255)
                                                                                                                                   tGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt             <  1:2146687/148‑1 (MQ=255)
                                                                                                                                   tGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt             <  2:1215967/148‑1 (MQ=255)
                                                                                                                                   tGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt             >  1:2509895/1‑148 (MQ=255)
                                                                                                                                     ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg           <  2:2823210/148‑1 (MQ=255)
                                                                                                                                       cGCCATATCCTCGCGCAGAAAATCCATCCGTCGATCATCTTCTGGTCGCTGGGATATGAATCAGGCTATGGCTTTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGTATGACACGCGTCTGGTGCATTACTAAGAAGATcgcg         <  2:1833250/148‑1 (MQ=255)
                                                                                                                                       cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg         <  2:2110669/148‑1 (MQ=255)
                                                                                                                                        gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGa        <  1:3276608/148‑1 (MQ=255)
                                                                                                                                          cATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg      <  1:2819762/148‑1 (MQ=255)
                                                                                                                                           atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc     >  1:290920/1‑148 (MQ=255)
                                                                                                                                           atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAAATCCGCGCGATGTACCATGCGGCGAAACCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATAGCGAttc     >  1:1454610/1‑146 (MQ=255)
                                                                                                                                            tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt    >  1:529267/1‑148 (MQ=255)
                                                                                                                                             atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTg   >  2:1905251/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA  >  CP009273/3217144‑3217433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAG  >  CP009273/3217140‑3217435
                                                                                                                                                     |                                                                                                                                                  
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCT                                                                                                                                                    <  SRR19973069.2102857/150‑1 (MQ=60)
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCT                                                                                                                                                    <  SRR19973069.117234/150‑1 (MQ=60)
 TACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTC                                                                                                                                                   <  SRR19973069.3311279/150‑1 (MQ=60)
 TACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTC                                                                                                                                                   <  SRR19973069.3462313/150‑1 (MQ=60)
  ACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCG                                                                                                                                                  <  SRR19973069.714942/150‑1 (MQ=60)
    GAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCG                                                                                                                                                <  SRR19973069.2801427/150‑1 (MQ=60)
      ACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCA                                                                                                                                              >  SRR19973069.1588783/1‑150 (MQ=60)
      ACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCA                                                                                                                                              >  SRR19973069.1589013/1‑150 (MQ=60)
           GTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAA                                                                                                                                         <  SRR19973069.3049230/150‑1 (MQ=60)
              ATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACC                                                                                                                                      >  SRR19973069.504683/1‑150 (MQ=60)
                ATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAT                                                                                                                                    <  SRR19973069.2687171/150‑1 (MQ=60)
                ATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAT                                                                                                                                    <  SRR19973069.1403688/150‑1 (MQ=60)
                 TCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATC                                                                                                                                   >  SRR19973069.3590762/1‑150 (MQ=60)
                 TCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATC                                                                                                                                   >  SRR19973069.3590781/1‑150 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                  >  SRR19973069.1294246/1‑150 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                  >  SRR19973069.1294198/1‑150 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                  >  SRR19973069.1113640/1‑150 (MQ=60)
                  CTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCC                                                                                                                                  >  SRR19973069.1113582/1‑150 (MQ=60)
                    ACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGT                                                                                                                                >  SRR19973069.2125225/1‑150 (MQ=60)
                     CGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGTATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTC                                                                                                                               >  SRR19973069.2482415/1‑150 (MQ=60)
                     CGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGTATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTC                                                                                                                               >  SRR19973069.550486/1‑150 (MQ=60)
                          TGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCA                                                                                                                          <  SRR19973069.2920038/150‑1 (MQ=60)
                          TGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCA                                                                                                                          <  SRR19973069.996461/150‑1 (MQ=60)
                               GTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATC                                                                                                                     >  SRR19973069.1230212/1‑150 (MQ=60)
                                 GATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTG                                                                                                                   <  SRR19973069.3802457/150‑1 (MQ=60)
                                   TGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGT                                                                                                                 <  SRR19973069.2972710/150‑1 (MQ=60)
                                   TGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGT                                                                                                                 >  SRR19973069.176179/1‑150 (MQ=60)
                                       GGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCT                                                                                                             <  SRR19973069.1713274/150‑1 (MQ=60)
                                       GGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCT                                                                                                             >  SRR19973069.1771803/1‑150 (MQ=60)
                                       GGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCT                                                                                                             >  SRR19973069.1771825/1‑150 (MQ=60)
                                         AAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGG                                                                                                           >  SRR19973069.1475836/1‑150 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                          >  SRR19973069.281370/1‑150 (MQ=60)
                                             CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAA                                                                                                       >  SRR19973069.2622308/1‑150 (MQ=60)
                                                  TCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAT                                                                                                  >  SRR19973069.664951/1‑150 (MQ=60)
                                                    GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCC                                                                                                <  SRR19973069.574979/150‑1 (MQ=60)
                                                    GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCC                                                                                                <  SRR19973069.2508015/150‑1 (MQ=60)
                                                          CACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAT                                                                                          >  SRR19973069.1832883/1‑150 (MQ=60)
                                                          CACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAT                                                                                          >  SRR19973069.1579645/1‑150 (MQ=60)
                                                           ACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATG                                                                                         <  SRR19973069.1151441/150‑1 (MQ=60)
                                                             GGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGC                                                                                       >  SRR19973069.35919/1‑150 (MQ=60)
                                                             GGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGC                                                                                       >  SRR19973069.36000/1‑150 (MQ=60)
                                                               CTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTG                                                                                     >  SRR19973069.2446667/1‑150 (MQ=60)
                                                                   GCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAC                                                                                 <  SRR19973069.2614478/150‑1 (MQ=60)
                                                                     TAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAT                                                                               <  SRR19973069.3838652/150‑1 (MQ=60)
                                                                               GATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATG                                                                     >  SRR19973069.2015567/1‑150 (MQ=60)
                                                                               GATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATG                                                                     >  SRR19973069.2209890/1‑150 (MQ=60)
                                                                                ATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGT                                                                    >  SRR19973069.2036834/1‑150 (MQ=60)
                                                                                 TATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTA                                                                   >  SRR19973069.107823/1‑150 (MQ=60)
                                                                                 TATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTA                                                                   >  SRR19973069.1718839/1‑150 (MQ=60)
                                                                                  ATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAC                                                                  >  SRR19973069.433268/1‑150 (MQ=60)
                                                                                   TTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACC                                                                 >  SRR19973069.315135/1‑150 (MQ=60)
                                                                                   TTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACC                                                                 <  SRR19973069.3610018/150‑1 (MQ=60)
                                                                                    TAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCA                                                                >  SRR19973069.416838/1‑150 (MQ=60)
                                                                                    TAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCA                                                                >  SRR19973069.1107171/1‑150 (MQ=60)
                                                                                     AGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAT                                                               >  SRR19973069.114616/1‑150 (MQ=60)
                                                                                      GCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATG                                                              <  SRR19973069.3699476/150‑1 (MQ=60)
                                                                                      GCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATG                                                              <  SRR19973069.2797183/150‑1 (MQ=60)
                                                                                      GCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATG                                                              <  SRR19973069.2797206/150‑1 (MQ=60)
                                                                                        CGTATTACCGACGATCCTCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCG                                                            <  SRR19973069.3221391/150‑1 (MQ=60)
                                                                                        CGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCG                                                            <  SRR19973069.2703795/150‑1 (MQ=60)
                                                                                           ATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCACTGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCG                                                         >  SRR19973069.480920/1‑150 (MQ=60)
                                                                                           ATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCG                                                         >  SRR19973069.1691455/1‑150 (MQ=60)
                                                                                           ATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCG                                                         >  SRR19973069.481139/1‑150 (MQ=60)
                                                                                           ATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCG                                                         >  SRR19973069.481166/1‑150 (MQ=60)
                                                                                              ACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAA                                                      >  SRR19973069.419805/1‑150 (MQ=60)
                                                                                              ACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAA                                                      >  SRR19973069.420657/1‑150 (MQ=60)
                                                                                              ACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAA                                                      >  SRR19973069.419659/1‑150 (MQ=60)
                                                                                                CGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGC                                                    <  SRR19973069.2308184/150‑1 (MQ=60)
                                                                                                   CGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCT                                                 <  SRR19973069.2010914/150‑1 (MQ=60)
                                                                                                     ATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGG                                               <  SRR19973069.2011173/150‑1 (MQ=60)
                                                                                                        CGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATG                                            <  SRR19973069.3478115/150‑1 (MQ=60)
                                                                                                         GCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGA                                           <  SRR19973069.2556174/150‑1 (MQ=60)
                                                                                                         GCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGA                                           <  SRR19973069.2556181/150‑1 (MQ=60)
                                                                                                          CAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGAC                                          <  SRR19973069.2408176/150‑1 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                         >  SRR19973069.285230/1‑150 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                         >  SRR19973069.285261/1‑150 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGATGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                         <  SRR19973069.1341278/150‑1 (MQ=60)
                                                                                                           AGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGATGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACA                                         <  SRR19973069.1341412/150‑1 (MQ=60)
                                                                                                            GTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAC                                        >  SRR19973069.2997859/1‑150 (MQ=60)
                                                                                                            GTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAC                                        <  SRR19973069.2562483/150‑1 (MQ=60)
                                                                                                                  AAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACT                                  >  SRR19973069.3123121/1‑150 (MQ=60)
                                                                                                                  AAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACT                                  >  SRR19973069.919887/1‑150 (MQ=60)
                                                                                                                  AAAAGTCTACGTCGAGCGCATTGTTCGCCATATCATCGAGCAGAAAAACCATCCGTCGATCATAATCAGGTCTCTGGGCAATGAATCCGGCTAAGCCTGTAACATCCTCGCGATGTAACATGCGGCGAAAGCGCTGGATGACACGCGACT                                  >  SRR19973069.3123139/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                              >  SRR19973069.755589/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                              >  SRR19973069.755418/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                              >  SRR19973069.257139/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                              >  SRR19973069.755511/1‑150 (MQ=60)
                                                                                                                       TCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGC                             >  SRR19973069.980260/1‑150 (MQ=60)
                                                                                                                        CTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCA                            <  SRR19973069.679115/150‑1 (MQ=60)
                                                                                                                            GTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAC                        <  SRR19973069.508137/150‑1 (MQ=60)
                                                                                                                            GTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAC                        <  SRR19973069.508216/150‑1 (MQ=60)
                                                                                                                            GTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAC                        <  SRR19973069.181314/150‑1 (MQ=60)
                                                                                                                             TCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACG                       <  SRR19973069.1856146/150‑1 (MQ=60)
                                                                                                                                AGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAG                    <  SRR19973069.3369845/150‑1 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                  <  SRR19973069.688256/150‑1 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                  >  SRR19973069.1079278/1‑150 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                  <  SRR19973069.2618665/150‑1 (MQ=60)
                                                                                                                                    CATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGA                >  SRR19973069.1890467/1‑150 (MQ=60)
                                                                                                                                    CATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGA                >  SRR19973069.1890671/1‑150 (MQ=60)
                                                                                                                                     ATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAT               >  SRR19973069.2059317/1‑150 (MQ=60)
                                                                                                                                     ATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAT               >  SRR19973069.2526621/1‑150 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG             >  SRR19973069.3011064/1‑150 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG             <  SRR19973069.2161381/150‑1 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG             <  SRR19973069.2161408/150‑1 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG             <  SRR19973069.2161600/150‑1 (MQ=60)
                                                                                                                                       TGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCG             <  SRR19973069.2161547/150‑1 (MQ=60)
                                                                                                                                           CGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAT         <  SRR19973069.1502947/150‑1 (MQ=60)
                                                                                                                                            GCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATG        <  SRR19973069.3298062/150‑1 (MQ=60)
                                                                                                                                             CCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGC       >  SRR19973069.292923/1‑150 (MQ=60)
                                                                                                                                             CCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGC       >  SRR19973069.1464784/1‑150 (MQ=60)
                                                                                                                                             CCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAAATCCGCGCGATGTACCATGCGGCGAAACCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATAGCGATTC       >  SRR19973069.1464763/1‑150 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT      >  SRR19973069.533000/1‑150 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT      <  SRR19973069.2643244/150‑1 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT      <  SRR19973069.2838416/150‑1 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT      <  SRR19973069.2838423/150‑1 (MQ=60)
                                                                                                                                              CATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT      <  SRR19973069.748683/150‑1 (MQ=60)
                                                                                                                                                 ATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAA   >  SRR19973069.3566558/1‑150 (MQ=60)
                                                                                                                                                 ATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAA   >  SRR19973069.1353384/1‑150 (MQ=60)
                                                                                                                                                  TCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAG  <  SRR19973069.1727109/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAG  >  CP009273/3217140‑3217435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑