Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,217,289 A→T 55.5% H433L (CAC→CTC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,217,2890AT55.5% 128.8 / inf 283H433L (CAC→CTC) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (78/48);  new base T (78/79);  total (156/127)
Fisher's exact test for biased strand distribution p-value = 4.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437
                                                                                                                                                     |                                                                                                                                                    
ttACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCa                                                                                                                                                      >  1:1920645/1‑150 (MQ=255)
 tACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAc                                                                                                                                                     <  2:2822933/150‑1 (MQ=255)
  aCGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcg                                                                                                                                                    <  2:3142042/150‑1 (MQ=255)
   cGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcgc                                                                                                                                                   >  2:286262/1‑150 (MQ=255)
         gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                             >  2:3339264/1‑150 (MQ=255)
         gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                             >  1:1573096/1‑150 (MQ=255)
          tgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaa                                                                                                                                            >  2:3583603/1‑150 (MQ=255)
           gtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaaaa                                                                                                                                           <  2:2409162/150‑1 (MQ=255)
               tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCa                                                                                                                                       >  1:1370654/1‑150 (MQ=255)
                 tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATc                                                                                                                                     >  2:2618737/1‑150 (MQ=255)
                 tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATc                                                                                                                                     <  1:2454968/150‑1 (MQ=255)
                 tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATc                                                                                                                                     <  2:440708/150‑1 (MQ=255)
                   tACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCg                                                                                                                                   >  1:3008271/1‑150 (MQ=255)
                        ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGat                                                                                                                              <  2:624085/150‑1 (MQ=255)
                         cTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatc                                                                                                                             <  1:2026765/150‑1 (MQ=255)
                          tGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatca                                                                                                                            >  2:3794948/1‑150 (MQ=255)
                           ttttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcat                                                                                                                           >  2:3103916/2‑150 (MQ=255)
                            tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatc                                                                                                                          <  1:4143749/150‑1 (MQ=255)
                                tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCt                                                                                                                      <  1:612065/150‑1 (MQ=255)
                                 gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTg                                                                                                                     <  2:1763381/150‑1 (MQ=255)
                                    ggCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTc                                                                                                                  <  1:2613054/150‑1 (MQ=255)
                                        gAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTg                                                                                                              >  2:1191288/1‑150 (MQ=255)
                                         aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCAGATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                             <  1:3956828/150‑1 (MQ=255)
                                               aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATg                                                                                                       <  2:2549708/150‑1 (MQ=255)
                                                 gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                     >  1:2845463/1‑150 (MQ=255)
                                                    gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATcc                                                                                                  >  1:1276827/1‑150 (MQ=255)
                                                     aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAATAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                                 >  2:1514432/1‑150 (MQ=255)
                                                       tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTGTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                               >  1:4118475/1‑150 (MQ=255)
                                                       tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                               >  1:679568/1‑150 (MQ=255)
                                                       tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                               >  2:4346588/1‑150 (MQ=255)
                                                        cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                              <  2:3624832/150‑1 (MQ=255)
                                                        cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                              <  2:1462765/150‑1 (MQ=255)
                                                           aCGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATg                                                                                           <  1:1120029/150‑1 (MQ=255)
                                                             ggCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGc                                                                                         <  1:1190227/150‑1 (MQ=255)
                                                             ggCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGc                                                                                         <  2:3917466/150‑1 (MQ=255)
                                                               cTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTg                                                                                       >  2:404560/1‑150 (MQ=255)
                                                               cTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTg                                                                                       >  2:4289157/1‑150 (MQ=255)
                                                                tttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                      <  1:1162998/150‑1 (MQ=255)
                                                                tttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                      >  2:3883085/1‑150 (MQ=255)
                                                                 ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTa                                                                                     <  1:3062912/150‑1 (MQ=255)
                                                                   gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                                   >  1:539747/1‑150 (MQ=255)
                                                                     tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                                 >  2:1455983/1‑150 (MQ=255)
                                                                      aaTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGACTCCGGCTATGGCTGTAACATc                                                                                >  2:2280653/1‑150 (MQ=255)
                                                                        tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcg                                                                              >  2:578819/1‑150 (MQ=255)
                                                                        tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcg                                                                              >  2:1849820/1‑150 (MQ=255)
                                                                         gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgc                                                                             >  1:1043554/1‑150 (MQ=255)
                                                                          tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcg                                                                            <  2:1111609/150‑1 (MQ=255)
                                                                           cGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcgc                                                                           >  2:3737931/1‑150 (MQ=255)
                                                                            ggCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcgcg                                                                          <  2:2225776/150‑1 (MQ=255)
                                                                              cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAt                                                                        <  2:3067409/150‑1 (MQ=255)
                                                                              cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAt                                                                        >  2:1065310/1‑150 (MQ=255)
                                                                               gATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                       >  2:1249671/1‑150 (MQ=255)
                                                                                atatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAtat                                                                      >  2:3193626/1‑148 (MQ=255)
                                                                                 tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                     >  1:4230579/1‑150 (MQ=255)
                                                                                   ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAcc                                                                   >  1:548277/1‑150 (MQ=255)
                                                                                    tAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                                  <  2:1433126/150‑1 (MQ=255)
                                                                                     aGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAt                                                                 >  1:2705502/1‑150 (MQ=255)
                                                                                     aGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAt                                                                 >  2:802885/1‑150 (MQ=255)
                                                                                       ccGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                               >  1:3274612/1‑150 (MQ=255)
                                                                                       ccGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                               >  1:571010/1‑150 (MQ=255)
                                                                                        cGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcg                                                              >  2:849731/1‑150 (MQ=255)
                                                                                        cGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcg                                                              <  2:4114425/150‑1 (MQ=255)
                                                                                         gTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcgg                                                             >  2:2711185/1‑150 (MQ=255)
                                                                                          tATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggc                                                            <  2:292788/150‑1 (MQ=255)
                                                                                             tACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                         <  2:4346364/150‑1 (MQ=255)
                                                                                              aCCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaaa                                                        >  1:1981950/1‑150 (MQ=255)
                                                                                                cgacgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgc                                                      <  1:2696590/150‑1 (MQ=255)
                                                                                                cgacgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgc                                                      <  1:1702386/150‑1 (MQ=255)
                                                                                                   cgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                                   <  1:3351850/150‑1 (MQ=255)
                                                                                                    gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTg                                                  <  1:1001051/150‑1 (MQ=255)
                                                                                                      tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGa                                                >  2:3983944/1‑150 (MQ=255)
                                                                                                       ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGAt                                               <  2:3594931/150‑1 (MQ=255)
                                                                                                         gCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGa                                             <  1:3374983/150‑1 (MQ=255)
                                                                                                            gtgtGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGacac                                          <  1:389408/150‑1 (MQ=255)
                                                                                                               ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                       <  1:1614538/150‑1 (MQ=255)
                                                                                                                gAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGa                                      >  1:3314410/1‑150 (MQ=255)
                                                                                                                  aaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACt                                    <  2:3256353/150‑1 (MQ=255)
                                                                                                                  aaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACt                                    <  2:133560/150‑1 (MQ=255)
                                                                                                                    aaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTgg                                  >  2:3702158/1‑150 (MQ=255)
                                                                                                                      gTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                                <  1:953959/150‑1 (MQ=255)
                                                                                                                        cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                              <  2:2216828/150‑1 (MQ=255)
                                                                                                                        cTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                              >  1:2075920/1‑150 (MQ=255)
                                                                                                                         tACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAt                             >  1:238123/1‑150 (MQ=255)
                                                                                                                           cGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTa                           >  1:3395357/1‑150 (MQ=255)
                                                                                                                            gTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAc                          <  1:2259631/150‑1 (MQ=255)
                                                                                                                                aGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaag                      >  1:3464872/1‑150 (MQ=255)
                                                                                                                                 gcgcATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaaga                     <  1:802885/150‑1 (MQ=255)
                                                                                                                                  cgcATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaagac                    >  2:3746813/1‑149 (MQ=255)
                                                                                                                                  cgcATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaagaa                    <  1:3627354/150‑1 (MQ=255)
                                                                                                                                   gcATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGaagaag                   >  2:2999023/1‑150 (MQ=255)
                                                                                                                                    cATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga                  >  2:1893521/1‑150 (MQ=255)
                                                                                                                                     aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt                 <  1:3234842/150‑1 (MQ=255)
                                                                                                                                     aTTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATTCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt                 >  2:294533/1‑150 (MQ=255)
                                                                                                                                        gTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgc              >  1:3868923/1‑150 (MQ=255)
                                                                                                                                        gTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgc              <  2:2010849/150‑1 (MQ=255)
                                                                                                                                         ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg             <  1:2874174/150‑1 (MQ=255)
                                                                                                                                            gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg          <  1:4322333/150‑1 (MQ=255)
                                                                                                                                                tatCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGa      <  2:1370654/150‑1 (MQ=255)
                                                                                                                                                 atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa     >  2:1639516/1‑150 (MQ=255)
                                                                                                                                                 atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa     >  2:3440339/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437
                                                                                                                                                     |                                                                                                                                                    
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCA                                                                                                                                                      >  SRR19973079.1930900/1‑150 (MQ=60)
 TACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAC                                                                                                                                                     >  SRR19973079.1290310/1‑150 (MQ=60)
   CGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGC                                                                                                                                                   >  SRR19973079.1632704/1‑150 (MQ=60)
      ACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCA                                                                                                                                                <  SRR19973079.969077/150‑1 (MQ=60)
       CTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAG                                                                                                                                               >  SRR19973079.4339014/1‑150 (MQ=60)
       CTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAG                                                                                                                                               >  SRR19973079.3896057/1‑150 (MQ=60)
         GTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAA                                                                                                                                             >  SRR19973079.1581778/1‑150 (MQ=60)
          TGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAA                                                                                                                                            <  SRR19973079.2311718/150‑1 (MQ=60)
          TGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAA                                                                                                                                            >  SRR19973079.833322/1‑150 (MQ=60)
            TGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAA                                                                                                                                          >  SRR19973079.3272799/1‑150 (MQ=60)
              ATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACC                                                                                                                                        >  SRR19973079.1019103/1‑150 (MQ=60)
               TATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCA                                                                                                                                       >  SRR19973079.243505/1‑150 (MQ=60)
               TATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCA                                                                                                                                       >  SRR19973079.1378254/1‑150 (MQ=60)
                 TCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATC                                                                                                                                     <  SRR19973079.2467553/150‑1 (MQ=60)
                   TACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCG                                                                                                                                   >  SRR19973079.3023810/1‑150 (MQ=60)
                        CCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGAT                                                                                                                              >  SRR19973079.1777282/1‑150 (MQ=60)
                         CTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATC                                                                                                                             <  SRR19973079.2037432/150‑1 (MQ=60)
                         CTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATC                                                                                                                             <  SRR19973079.2037389/150‑1 (MQ=60)
                          TGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCA                                                                                                                            >  SRR19973079.794737/1‑150 (MQ=60)
                          TGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCA                                                                                                                            <  SRR19973079.789076/150‑1 (MQ=60)
                            TTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATC                                                                                                                          <  SRR19973079.4164813/150‑1 (MQ=60)
                            TTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATC                                                                                                                          <  SRR19973079.4164775/150‑1 (MQ=60)
                             TTGTGATGGCGGAAACCGACGTCGAATCGCTCGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCA                                                                                                                         <  SRR19973079.2630157/150‑1 (MQ=60)
                                TGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCT                                                                                                                      <  SRR19973079.615457/150‑1 (MQ=60)
                                TGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCT                                                                                                                      <  SRR19973079.615605/150‑1 (MQ=60)
                                TGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCT                                                                                                                      <  SRR19973079.1960646/150‑1 (MQ=60)
                                  ATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGG                                                                                                                    >  SRR19973079.340642/1‑150 (MQ=60)
                                  ATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGAGATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGG                                                                                                                    >  SRR19973079.2527047/1‑150 (MQ=60)
                                    GGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTC                                                                                                                  <  SRR19973079.2626519/150‑1 (MQ=60)
                                         AAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCAGATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGG                                                                                                             <  SRR19973079.3976756/150‑1 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                            >  SRR19973079.3154604/1‑150 (MQ=60)
                                          AACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGG                                                                                                            >  SRR19973079.3816306/1‑150 (MQ=60)
                                            CCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCA                                                                                                          <  SRR19973079.2342917/150‑1 (MQ=60)
                                             CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAATTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAA                                                                                                         <  SRR19973079.3467733/150‑1 (MQ=60)
                                              GACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAT                                                                                                        >  SRR19973079.3037055/1‑150 (MQ=60)
                                                CGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGA                                                                                                      >  SRR19973079.3949114/1‑150 (MQ=60)
                                                 GTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAA                                                                                                     >  SRR19973079.674237/1‑150 (MQ=60)
                                                 GTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAA                                                                                                     >  SRR19973079.2859955/1‑150 (MQ=60)
                                                    GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCC                                                                                                  <  SRR19973079.1113474/150‑1 (MQ=60)
                                                    GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCC                                                                                                  >  SRR19973079.1284122/1‑150 (MQ=60)
                                                       TCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTGTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGC                                                                                               >  SRR19973079.4139376/1‑150 (MQ=60)
                                                       TCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGC                                                                                               >  SRR19973079.683311/1‑150 (MQ=60)
                                                        CGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCT                                                                                              >  SRR19973079.2106035/1‑150 (MQ=60)
                                                        CGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCT                                                                                              >  SRR19973079.3054704/1‑150 (MQ=60)
                                                           ACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATG                                                                                           <  SRR19973079.1126379/150‑1 (MQ=60)
                                                           ACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATG                                                                                           <  SRR19973079.1771787/150‑1 (MQ=60)
                                                             GGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGC                                                                                         <  SRR19973079.1197084/150‑1 (MQ=60)
                                                             GGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGC                                                                                         >  SRR19973079.2021468/1‑150 (MQ=60)
                                                              GCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCT                                                                                        >  SRR19973079.2514485/1‑150 (MQ=60)
                                                                TTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGT                                                                                      <  SRR19973079.936744/150‑1 (MQ=60)
                                                                TTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGT                                                                                      <  SRR19973079.1169696/150‑1 (MQ=60)
                                                                 TTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTA                                                                                     <  SRR19973079.1226670/150‑1 (MQ=60)
                                                                 TTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTA                                                                                     <  SRR19973079.3896995/150‑1 (MQ=60)
                                                                 TTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTA                                                                                     >  SRR19973079.1612722/1‑150 (MQ=60)
                                                                 TTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTA                                                                                     <  SRR19973079.4212490/150‑1 (MQ=60)
                                                                 TTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTA                                                                                     <  SRR19973079.3078770/150‑1 (MQ=60)
                                                                  TGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAA                                                                                    <  SRR19973079.2068550/150‑1 (MQ=60)
                                                                   GCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAC                                                                                   >  SRR19973079.1676894/1‑150 (MQ=60)
                                                                   GCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAC                                                                                   >  SRR19973079.543057/1‑150 (MQ=60)
                                                                   GCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAC                                                                                   >  SRR19973079.542985/1‑150 (MQ=60)
                                                                         GTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGC                                                                             >  SRR19973079.1049323/1‑150 (MQ=60)
                                                                              CGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAT                                                                        >  SRR19973079.1963547/1‑150 (MQ=60)
                                                                              CGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAT                                                                        >  SRR19973079.4162864/1‑150 (MQ=60)
                                                                                ATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGT                                                                      >  SRR19973079.1434991/1‑150 (MQ=60)
                                                                                ATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGT                                                                      >  SRR19973079.3556631/1‑150 (MQ=60)
                                                                                 TATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTA                                                                     >  SRR19973079.3439682/1‑150 (MQ=60)
                                                                                 TATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTA                                                                     >  SRR19973079.4251982/1‑150 (MQ=60)
                                                                                   TTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACC                                                                   >  SRR19973079.550918/1‑150 (MQ=60)
                                                                                   TTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACC                                                                   >  SRR19973079.551553/1‑150 (MQ=60)
                                                                                    TAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCA                                                                  >  SRR19973079.2771978/1‑150 (MQ=60)
                                                                                     AGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAT                                                                 >  SRR19973079.2719505/1‑150 (MQ=60)
                                                                                     AGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAT                                                                 >  SRR19973079.2719498/1‑150 (MQ=60)
                                                                                       CCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGC                                                               >  SRR19973079.3291176/1‑150 (MQ=60)
                                                                                       CCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGC                                                               >  SRR19973079.574389/1‑150 (MQ=60)
                                                                                              ACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAA                                                        >  SRR19973079.1992466/1‑150 (MQ=60)
                                                                                                CGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGC                                                      <  SRR19973079.2710550/150‑1 (MQ=60)
                                                                                                CGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGC                                                      <  SRR19973079.1711504/150‑1 (MQ=60)
                                                                                                 GACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCG                                                     <  SRR19973079.4160020/150‑1 (MQ=60)
                                                                                                   CGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCT                                                   <  SRR19973079.3368612/150‑1 (MQ=60)
                                                                                                   CGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCT                                                   <  SRR19973079.3369061/150‑1 (MQ=60)
                                                                                                    GATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTG                                                  <  SRR19973079.1006699/150‑1 (MQ=60)
                                                                                                     ATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGG                                                 >  SRR19973079.294542/1‑150 (MQ=60)
                                                                                                     ATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGG                                                 >  SRR19973079.3553226/1‑150 (MQ=60)
                                                                                                      TCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGA                                                <  SRR19973079.3760378/150‑1 (MQ=60)
                                                                                                         GCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGA                                             <  SRR19973079.3392377/150‑1 (MQ=60)
                                                                                                         GCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGA                                             <  SRR19973079.3392294/150‑1 (MQ=60)
                                                                                                         GCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGA                                             <  SRR19973079.3392613/150‑1 (MQ=60)
                                                                                                            GTGTGAAAAAGTCTACGTCGAGCGCATTGTTCGACATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAC                                          <  SRR19973079.391632/150‑1 (MQ=60)
                                                                                                            GTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAC                                          <  SRR19973079.511913/150‑1 (MQ=60)
                                                                                                             TGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACG                                         >  SRR19973079.1179624/1‑150 (MQ=60)
                                                                                                             TGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACG                                         >  SRR19973079.1179617/1‑150 (MQ=60)
                                                                                                               GGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCG                                       <  SRR19973079.1623307/150‑1 (MQ=60)
                                                                                                               GGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCG                                       <  SRR19973079.1623373/150‑1 (MQ=60)
                                                                                                                GAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGA                                      >  SRR19973079.3331335/1‑150 (MQ=60)
                                                                                                                   AAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTG                                   >  SRR19973079.3271482/1‑150 (MQ=60)
                                                                                                                    AAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGG                                  <  SRR19973079.2632247/150‑1 (MQ=60)
                                                                                                                    AAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGG                                  <  SRR19973079.2955669/150‑1 (MQ=60)
                                                                                                                     AGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGACTGGT                                 >  SRR19973079.449981/1‑150 (MQ=60)
                                                                                                                      GTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTG                                <  SRR19973079.959420/150‑1 (MQ=60)
                                                                                                                        CTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCA                              >  SRR19973079.2086773/1‑150 (MQ=60)
                                                                                                                        CTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCA                              >  SRR19973079.2086725/1‑150 (MQ=60)
                                                                                                                         TACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAT                             >  SRR19973079.239714/1‑150 (MQ=60)
                                                                                                                         TACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAT                             >  SRR19973079.239830/1‑150 (MQ=60)
                                                                                                                           CGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTA                           <  SRR19973079.4069248/150‑1 (MQ=60)
                                                                                                                           CGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTA                           >  SRR19973079.598426/1‑150 (MQ=60)
                                                                                                                           CGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTA                           >  SRR19973079.3412869/1‑150 (MQ=60)
                                                                                                                           CGTCGAGCGCATTGTTCGCAATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTA                           <  SRR19973079.1198147/150‑1 (MQ=60)
                                                                                                                            GTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTTGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAC                          >  SRR19973079.2765418/1‑150 (MQ=60)
                                                                                                                            GTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAC                          <  SRR19973079.2271346/150‑1 (MQ=60)
                                                                                                                                AGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAG                      >  SRR19973079.3482738/1‑150 (MQ=60)
                                                                                                                                AGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAG                      <  SRR19973079.4317625/150‑1 (MQ=60)
                                                                                                                                 GCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGA                     <  SRR19973079.807475/150‑1 (MQ=60)
                                                                                                                                  CGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAA                    <  SRR19973079.3645693/150‑1 (MQ=60)
                                                                                                                                    CATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGA                  >  SRR19973079.632500/1‑150 (MQ=60)
                                                                                                                                     ATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAT                 <  SRR19973079.3251241/150‑1 (MQ=60)
                                                                                                                                     ATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAT                 <  SRR19973079.3250660/150‑1 (MQ=60)
                                                                                                                                     ATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAAGAAGAAGAT                 >  SRR19973079.1103675/1‑150 (MQ=60)
                                                                                                                                        GTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGC              >  SRR19973079.3983779/1‑150 (MQ=60)
                                                                                                                                        GTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGC              >  SRR19973079.3888611/1‑150 (MQ=60)
                                                                                                                                         TTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCG             <  SRR19973079.1168042/150‑1 (MQ=60)
                                                                                                                                         TTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCG             <  SRR19973079.2888737/150‑1 (MQ=60)
                                                                                                                                            GCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATG          <  SRR19973079.3322802/150‑1 (MQ=60)
                                                                                                                                            GCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATG          <  SRR19973079.3322822/150‑1 (MQ=60)
                                                                                                                                            GCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATG          <  SRR19973079.4344072/150‑1 (MQ=60)
                                                                                                                                             CCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGC         <  SRR19973079.1169322/150‑1 (MQ=60)
                                                                                                                                             CCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGC         <  SRR19973079.3376534/150‑1 (MQ=60)
                                                                                                                                                TATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA      >  SRR19973079.1296399/1‑150 (MQ=60)
                                                                                                                                                TATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGA      <  SRR19973079.571759/150‑1 (MQ=60)
                                                                                                                                                 ATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAA     <  SRR19973079.2869584/150‑1 (MQ=60)
                                                                                                                                                    CACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  SRR19973079.2717690/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTG  >  CP009273/3217140‑3217437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑