Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,509,102 G→T 100% S57R (AGC→AGA)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,509,1020GT96.8% 576.1 / 7.5 190S57R (AGC→AGA) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (102/81);  total (107/83)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508957‑3509246
                                                                                                                                                 |                                                                                                                                                
ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                >  1:2712202/1‑148 (MQ=255)
ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                <  2:1419341/148‑1 (MQ=255)
ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                >  1:2494201/1‑148 (MQ=255)
 gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                               >  2:522306/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                              >  1:2659377/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                              >  1:3538652/1‑148 (MQ=255)
   acggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAtt                                                                                                                                             >  2:1800316/1‑148 (MQ=255)
    cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTg                                                                                                                                            <  1:2765198/148‑1 (MQ=255)
     ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGa                                                                                                                                           <  1:2916049/148‑1 (MQ=255)
     ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGa                                                                                                                                           <  1:552280/148‑1 (MQ=255)
      gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGat                                                                                                                                          >  2:191068/1‑148 (MQ=255)
       aTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                         >  2:79444/1‑148 (MQ=255)
       aTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                         >  2:3445559/1‑148 (MQ=255)
           tGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCgg                                                                                                                                     >  2:2545645/1‑148 (MQ=255)
            ggCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGc                                                                                                                                    >  2:2278281/1‑148 (MQ=255)
              cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCaa                                                                                                                                  <  1:576458/148‑1 (MQ=255)
              cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCaa                                                                                                                                  <  1:629685/148‑1 (MQ=255)
                tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAgg                                                                                                                                >  2:1035056/1‑148 (MQ=255)
                 ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGa                                                                                                                               <  2:2769676/148‑1 (MQ=255)
                 ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGa                                                                                                                               <  2:2769393/148‑1 (MQ=255)
                      gAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATaa                                                                                                                          <  1:1942039/148‑1 (MQ=255)
                         ctctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACga                                                                                                                       >  2:3345598/1‑148 (MQ=255)
                         ctctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACga                                                                                                                       >  2:1337921/1‑148 (MQ=255)
                         ctctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACga                                                                                                                       >  2:1866464/1‑148 (MQ=255)
                           ctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACgaga                                                                                                                     >  2:2690004/1‑148 (MQ=255)
                               gcgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAg                                                                                                                 <  1:3207277/148‑1 (MQ=255)
                               gcgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAg                                                                                                                 <  2:3206418/148‑1 (MQ=255)
                                cgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                                                <  1:2012186/148‑1 (MQ=255)
                                 ggAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTc                                                                                                               <  2:2388287/148‑1 (MQ=255)
                                 ggAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTc                                                                                                               >  2:438447/1‑148 (MQ=255)
                                  gAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCg                                                                                                              >  2:452300/1‑148 (MQ=255)
                                     cTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                                           <  1:2733979/148‑1 (MQ=255)
                                     cTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                                           <  1:1176851/148‑1 (MQ=255)
                                      tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                          <  1:793245/148‑1 (MQ=255)
                                      tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                          <  1:793093/148‑1 (MQ=255)
                                       gATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                                                         >  2:150964/1‑148 (MQ=255)
                                        aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        <  1:916011/148‑1 (MQ=255)
                                        aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        <  1:915961/148‑1 (MQ=255)
                                        aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        <  1:2147758/148‑1 (MQ=255)
                                             aaaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                   <  1:677461/148‑1 (MQ=255)
                                               aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAaca                                                                                                 <  1:1539642/148‑1 (MQ=255)
                                               aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAaca                                                                                                 <  2:2483816/148‑1 (MQ=255)
                                                ccTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAacac                                                                                                >  1:2210637/1‑148 (MQ=255)
                                                ccTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAacac                                                                                                >  1:2540129/1‑148 (MQ=255)
                                                  tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCg                                                                                              >  1:1117728/1‑148 (MQ=255)
                                                      cgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                          >  2:2094167/1‑148 (MQ=255)
                                                          aTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                      >  1:329130/1‑148 (MQ=255)
                                                           tttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGAGTTGAGAAACACCGACCCGGCAc                                                                                     <  2:2192982/148‑1 (MQ=255)
                                                            ttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAcc                                                                                    >  2:1322306/1‑148 (MQ=255)
                                                             tGTTTCGGGAAAAAACAGCTCGCGTGCGGCTTGTCGGTACTAATCTTTACGAATCTTCAAATTTTTATAGATACTGATATGTTATATTTTTATATCGGCATGTATATAACTAGAAATGTCGGTGTTGATAAACACCGACCCGGCACCt                                                                                   <  2:3015694/148‑1 (MQ=255)
                                                                ttCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACa                                                                                >  2:1357020/1‑148 (MQ=255)
                                                                       acaaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                                         >  1:718956/1‑148 (MQ=255)
                                                                         aaactGCTCGCGTGCGGCCTTTACGTACTAATCATTACGCATCTTCACAATTTTATAGAGAGTTATCTGGTCTATTTTTATATCGGCATGTTTTTAACTATAAATGTGGTTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCa                                                                       <  1:1800316/148‑1 (MQ=255)
                                                                          aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCaa                                                                      >  1:1653462/1‑148 (MQ=255)
                                                                           acaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                     >  2:3475997/1‑148 (MQ=255)
                                                                            caGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTAGAGAGACTGATCAGGTATCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                    <  2:1487531/148‑1 (MQ=255)
                                                                              gCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAg                                                                  >  2:2572607/1‑148 (MQ=255)
                                                                               cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGa                                                                 >  1:1208705/1‑148 (MQ=255)
                                                                               cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGa                                                                 >  1:2881098/1‑148 (MQ=255)
                                                                                 cgcgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACa                                                               >  1:1305856/1‑148 (MQ=255)
                                                                                  gcgTGCGGCCTGTACGTACTCATCAGTACGCATCTGCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGAcct                                                              >  2:2533228/1‑146 (MQ=255)
                                                                                     tGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCg                                                           >  1:3013174/1‑148 (MQ=255)
                                                                                        ggCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGccc                                                        <  2:1782412/148‑1 (MQ=255)
                                                                                             gtacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCATACATTCGCCCTTggg                                                   >  1:1057004/1‑148 (MQ=255)
                                                                                              tacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                                                  >  2:2505579/1‑148 (MQ=255)
                                                                                                cgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCTGGTCTCTTTTGTTATCGGCATGGATATAACGAGAAAAGTCGGTGTTGAGAACCACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                                                <  1:2344869/148‑1 (MQ=255)
                                                                                                    cTCATCAGTACGCATGTTCACAATGTTATAGAGAGTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                                            <  2:1018205/148‑1 (MQ=255)
                                                                                                    cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                                            >  1:2573472/1‑148 (MQ=255)
                                                                                                      catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          >  1:1105668/1‑148 (MQ=255)
                                                                                                      catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          >  2:1887735/1‑148 (MQ=255)
                                                                                                      catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          >  2:1884384/1‑148 (MQ=255)
                                                                                                      catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          >  2:2606313/1‑148 (MQ=255)
                                                                                                      catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          >  2:1050124/1‑148 (MQ=255)
                                                                                                        tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                        <  2:1915382/148‑1 (MQ=255)
                                                                                                         caGTACGCATCTTCACAATGTTATAGAGCCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       >  2:2904204/1‑148 (MQ=255)
                                                                                                         caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       <  2:2310621/148‑1 (MQ=255)
                                                                                                         caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       >  2:2904231/1‑148 (MQ=255)
                                                                                                            tACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTTATATCGGCAAGGATATACCGAGAAAAGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                    <  2:2712202/148‑1 (MQ=255)
                                                                                                            tACGCATCTTCACAATGTTATAGAGACTGAACAGGTCAATATTGATATCGGCAAGGATATAACGATAAAAGGGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                    <  2:630497/148‑1 (MQ=255)
                                                                                                             aCGCATCTTCACAAGTTTATAGAGAGTGATCAGGTCTCTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAata                                   <  2:3386415/148‑1 (MQ=255)
                                                                                                              cgaaTCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatat                                  <  2:1945573/145‑1 (MQ=255)
                                                                                                              agattCTTCACAATGTTTTAGATTCTGATCAGTTCTATATTGATATCGCCAAGGATATAACGAGAAAAGTCGGTGTTGATATACACCGCCCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatat                                  <  2:144239/144‑1 (MQ=255)
                                                                                                                  tCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcatc                              >  2:964364/1‑148 (MQ=255)
                                                                                                                   cTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca                             >  1:2252841/1‑148 (MQ=255)
                                                                                                                     tCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                           >  2:2596404/1‑148 (MQ=255)
                                                                                                                      cacaATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                          >  1:431233/1‑148 (MQ=255)
                                                                                                                      cacaATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                          >  1:1308936/1‑148 (MQ=255)
                                                                                                                            gTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGa                    >  2:2403084/1‑148 (MQ=255)
                                                                                                                              tataGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                  >  2:2300340/1‑148 (MQ=255)
                                                                                                                                 agagACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACtt               >  1:3431856/1‑148 (MQ=255)
                                                                                                                                   agaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGc             >  1:1078423/1‑148 (MQ=255)
                                                                                                                                      cTGATCAGTTATCTATTTATATCGGCAAGTATATAACGAGAAAAGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc          <  1:1739319/148‑1 (MQ=255)
                                                                                                                                      cTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc          <  2:1854425/148‑1 (MQ=255)
                                                                                                                                       ttaTCAGGTCTCTATTGTTATAGGCATGTATATAACGAGAAAAGTCGGTGTTGCGAACCACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGccccc         <  2:3192289/146‑1 (MQ=255)
                                                                                                                                        gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCt        >  1:2525363/1‑148 (MQ=255)
                                                                                                                                         aTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg       >  1:150323/1‑148 (MQ=255)
                                                                                                                                         aTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg       >  2:762761/1‑148 (MQ=255)
                                                                                                                                           cAGGTCTATATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc     <  1:2639954/148‑1 (MQ=255)
                                                                                                                                             ggTCTCTATTGATCTCGGCAAGGATATAACGAGAAAAGTCTGTGTTGAGAAACACCGACCCGGCACCTACACAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCa   <  2:3254519/148‑1 (MQ=255)
                                                                                                                                              gTCTCTATTGATATCGCCACGTATATAACGACAAAAGTGGGTTTTCACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAc  <  1:1035056/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508957‑3509246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248
                                                                                                                                                   |                                                                                                                                                  
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                  >  SRR19973066.2730417/1‑150 (MQ=60)
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                  >  SRR19973066.2730181/1‑150 (MQ=60)
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                  >  SRR19973066.2511204/1‑150 (MQ=60)
  GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                >  SRR19973066.1789239/1‑150 (MQ=60)
  GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                >  SRR19973066.2677203/1‑150 (MQ=60)
  GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                >  SRR19973066.3562171/1‑150 (MQ=60)
   GACCGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATT                                                                                                                                               >  SRR19973066.2265281/1‑150 (MQ=60)
    AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                              <  SRR19973066.32639/150‑1 (MQ=60)
      CGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGAT                                                                                                                                            <  SRR19973066.2783820/150‑1 (MQ=60)
       GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                           <  SRR19973066.2935505/150‑1 (MQ=60)
       GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                           <  SRR19973066.556247/150‑1 (MQ=60)
       GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATCGATA                                                                                                                                           <  SRR19973066.2933661/150‑1 (MQ=60)
          TCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCG                                                                                                                                        <  SRR19973066.1980178/150‑1 (MQ=60)
              GGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAA                                                                                                                                    >  SRR19973066.2962876/1‑150 (MQ=60)
               GCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAG                                                                                                                                   <  SRR19973066.2732911/150‑1 (MQ=60)
                CTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGG                                                                                                                                  <  SRR19973066.580515/150‑1 (MQ=60)
                CTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGG                                                                                                                                  <  SRR19973066.580561/150‑1 (MQ=60)
                CTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGG                                                                                                                                  <  SRR19973066.634204/150‑1 (MQ=60)
                      TTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAA                                                                                                                            <  SRR19973066.1628341/150‑1 (MQ=60)
                        GAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACG                                                                                                                          <  SRR19973066.1955719/150‑1 (MQ=60)
                        GAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACG                                                                                                                          <  SRR19973066.1955808/150‑1 (MQ=60)
                               TCGCGGAACTGATAGTAATCCTCGGCGAAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCGTGTACGTACTCATCAGTACGCATCTTCACTATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAG                                                                                                                   <  SRR19973066.3109635/150‑1 (MQ=60)
                                 GCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTC                                                                                                                 <  SRR19973066.3228549/150‑1 (MQ=60)
                                  CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                <  SRR19973066.2026285/150‑1 (MQ=60)
                                       CTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTT                                                                                                           <  SRR19973066.1185140/150‑1 (MQ=60)
                                       CTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTT                                                                                                           <  SRR19973066.2752366/150‑1 (MQ=60)
                                       CTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTT                                                                                                           <  SRR19973066.2752795/150‑1 (MQ=60)
                                        TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                          <  SRR19973066.799073/150‑1 (MQ=60)
                                        TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                          <  SRR19973066.799010/150‑1 (MQ=60)
                                        TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                          <  SRR19973066.798921/150‑1 (MQ=60)
                                          ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                        <  SRR19973066.922512/150‑1 (MQ=60)
                                          ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                        <  SRR19973066.2162663/150‑1 (MQ=60)
                                          ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                        <  SRR19973066.922539/150‑1 (MQ=60)
                                          ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                        <  SRR19973066.922565/150‑1 (MQ=60)
                                            AGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAA                                                                                                      >  SRR19973066.1211422/1‑150 (MQ=60)
                                               AAACCTCGGCGCAATTTGTTTCGGGAACACACAGCTCGCTTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACA                                                                                                   <  SRR19973066.670032/150‑1 (MQ=60)
                                               AAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACA                                                                                                   <  SRR19973066.172332/150‑1 (MQ=60)
                                               AAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACA                                                                                                   <  SRR19973066.682419/150‑1 (MQ=60)
                                                AACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAC                                                                                                  >  SRR19973066.2225952/1‑150 (MQ=60)
                                                AACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAC                                                                                                  >  SRR19973066.2557391/1‑150 (MQ=60)
                                                AACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAC                                                                                                  >  SRR19973066.2557349/1‑150 (MQ=60)
                                                 ACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACC                                                                                                 <  SRR19973066.1550638/150‑1 (MQ=60)
                                                  CCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCG                                                                                                >  SRR19973066.1125788/1‑150 (MQ=60)
                                                          CAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCA                                                                                        >  SRR19973066.331639/1‑150 (MQ=60)
                                                                      GGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGC                                                                            >  SRR19973066.1584648/1‑150 (MQ=60)
                                                                       GAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCT                                                                           >  SRR19973066.728100/1‑150 (MQ=60)
                                                                       GAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCT                                                                           >  SRR19973066.724275/1‑150 (MQ=60)
                                                                        AACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTC                                                                          >  SRR19973066.1290051/1‑150 (MQ=60)
                                                                          CAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAA                                                                        >  SRR19973066.1665502/1‑150 (MQ=60)
                                                                           AAACTGCTCGCGTGCGGCCTTTACGTACTAATCATTACGCATCTTCACAATTTTATAGAGAGTTATCTGGTCTATTTTTATATCGGCATGTTTTTAACTATAAATGTGGTTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAC                                                                       <  SRR19973066.1813110/150‑1 (MQ=31)
                                                                            AACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACC                                                                      >  SRR19973066.2502821/1‑150 (MQ=60)
                                                                               AGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGA                                                                   >  SRR19973066.2900392/1‑150 (MQ=60)
                                                                               AGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGA                                                                   >  SRR19973066.1108076/1‑150 (MQ=60)
                                                                               AGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGA                                                                   >  SRR19973066.1217153/1‑150 (MQ=60)
                                                                                 CTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACA                                                                 >  SRR19973066.1315325/1‑150 (MQ=60)
                                                                                     CGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCG                                                             >  SRR19973066.3033251/1‑150 (MQ=60)
                                                                                           ttCTGTACGTACTCATCATTCGGAATCTTCACATTTTTATAGAGACTGATCCGGTCTCTTTTGATAGCGGCAAGCATATAACGACAAAAGTGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTG                                                       <  SRR19973066.1445521/148‑1 (MQ=60)
                                                                                             CTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCATACATTCGCCCTTGGG                                                     >  SRR19973066.1064773/1‑150 (MQ=60)
                                                                                                  CGTACTCATCAGTACGAATCTTCACAATGTTATCGAGACTGATCTGGTCTCTTTTGTTATCGGCATGGATATAACGAGAAAAGTCGGTGTTGAGAACCACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAT                                                <  SRR19973066.2360824/150‑1 (MQ=60)
                                                                                                  CGAACTCATCAGTACGCATCTCCACAATGTTATAGAGACTGATCAGGTATCTTTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAT                                                <  SRR19973066.557221/150‑1 (MQ=60)
                                                                                                    TACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGC                                              >  SRR19973066.2590283/1‑150 (MQ=60)
                                                                                                    TACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGC                                              >  SRR19973066.2590928/1‑150 (MQ=60)
                                                                                                     ACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCC                                             >  SRR19973066.1416260/1‑150 (MQ=60)
                                                                                                      CTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCG                                            >  SRR19973066.1113680/1‑150 (MQ=60)
                                                                                                         ATCAGAACGCATCTACAAAATTTTATCGAGACGGATCTGGTCTATATTGATACCGCCAAGTTTATAACGACAAATGTCGGTGTTGACACACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTT                                         <  SRR19973066.2613185/150‑1 (MQ=60)
                                                                                                                   ATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCA                               >  SRR19973066.2268373/1‑150 (MQ=60)
                                                                                                                   ATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCA                               >  SRR19973066.845582/1‑150 (MQ=60)
                                                                                                                      TTCACAATGTTATAGAGCCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGC                            >  SRR19973066.1317744/1‑150 (MQ=60)
                                                                                                                      TTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGC                            >  SRR19973066.434563/1‑150 (MQ=60)
                                                                                                                      TTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGC                            >  SRR19973066.1318434/1‑150 (MQ=60)
                                                                                                                      TTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGC                            >  SRR19973066.434571/1‑150 (MQ=60)
                                                                                                                          CAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGG                        >  SRR19973066.1120257/1‑150 (MQ=60)
                                                                                                                          CAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGG                        >  SRR19973066.1548397/1‑150 (MQ=60)
                                                                                                                            ATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGA                      >  SRR19973066.2085572/1‑150 (MQ=60)
                                                                                                                             TTTTATAGAGACTGATCCGGTCTCTATTTATATCGGAAAGTATATAACGACAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAT                     <  SRR19973066.1177216/150‑1 (MQ=60)
                                                                                                                             TGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAT                     >  SRR19973066.3496715/1‑150 (MQ=60)
                                                                                                                                 ATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                 >  SRR19973066.3454815/1‑150 (MQ=60)
                                                                                                                                   AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC               >  SRR19973066.1086307/1‑150 (MQ=60)
                                                                                                                                   AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC               >  SRR19973066.1086271/1‑150 (MQ=60)
                                                                                                                                   AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC               >  SRR19973066.2877692/1‑150 (MQ=60)
                                                                                                                                       CCTGATCTGGTCTCTATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCC           <  SRR19973066.2660936/150‑1 (MQ=60)
                                                                                                                                        CTGATCAGTTATCTATTTATATCGGCAAGTATATAACGAGAAAAGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCT          <  SRR19973066.1751868/150‑1 (MQ=60)
                                                                                                                                        CTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCT          >  SRR19973066.2541631/1‑150 (MQ=60)
                                                                                                                                        CTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCT          >  SRR19973066.2542535/1‑150 (MQ=60)
                                                                                                                                         TGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTG         >  SRR19973066.151735/1‑150 (MQ=60)
                                                                                                                                           ATCCGGTCTCTATTGATATCGCCAAGGATATAACGACAACTGTGGGTGTTGACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC       <  SRR19973066.455777/150‑1 (MQ=60)
                                                                                                                                             CAGGTCTATATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCA     <  SRR19973066.2657716/150‑1 (MQ=60)
                                                                                                                                              AGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC    >  SRR19973066.953332/1‑150 (MQ=60)
                                                                                                                                               GGTCTCTATTTATAGCGGCAAGTATATAACGACAAATGGCGGTGTTGACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACT   <  SRR19973066.712146/150‑1 (MQ=60)
                                                                                                                                                GTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  SRR19973066.1605760/1‑150 (MQ=60)
                                                                                                                                                GTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  SRR19973066.2109802/1‑150 (MQ=60)
                                                                                                                                                GTCTCTATTGATATCGCCACGTATATAACGACAAAAGTGGGTTTTCACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  <  SRR19973066.1042674/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑