Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,509,102 G→T 100% S57R (AGC→AGA)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,509,1020GT99.2% 466.9 / ‑4.1 147S57R (AGC→AGA) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (84/61);  total (85/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 148 total aligned reads displayed.

GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCG‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508957‑3509246
                                                                                                                                                 |                                                                                                                                                 
ggaactgaTCCTGGCTTTTTTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTct‑ct                                                                                                                                                <  2:632789/148‑1 (MQ=255)
 gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTa                                                                                                                                               >  2:2497465/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCTGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              >  1:2593292/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              >  1:172570/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              >  2:3576884/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              >  1:2144417/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              >  1:2056149/1‑148 (MQ=255)
  aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTAt                                                                                                                                              <  1:1500237/148‑1 (MQ=255)
      gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGat                                                                                                                                          <  1:2698519/148‑1 (MQ=255)
       aTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCCGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGata                                                                                                                                         >  1:2087094/1‑148 (MQ=255)
        tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGatat                                                                                                                                        <  2:2274245/148‑1 (MQ=255)
        tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGatat                                                                                                                                        >  2:3257306/1‑148 (MQ=255)
        tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGatat                                                                                                                                        <  2:2274041/148‑1 (MQ=255)
              cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCaa                                                                                                                                  >  2:3182790/1‑148 (MQ=255)
                tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAgg                                                                                                                                <  2:894545/148‑1 (MQ=255)
                 ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGa                                                                                                                               >  2:2188008/1‑148 (MQ=255)
                  tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGat                                                                                                                              <  2:899599/148‑1 (MQ=255)
                  tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGat                                                                                                                              <  2:2630271/148‑1 (MQ=255)
                      gAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATaa                                                                                                                          <  1:2134521/148‑1 (MQ=255)
                        aCTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACg                                                                                                                        >  1:650725/1‑148 (MQ=255)
                          tctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACgag                                                                                                                      <  2:307237/148‑1 (MQ=255)
                          tctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACgag                                                                                                                      >  1:453955/1‑148 (MQ=255)
                             tCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGaaa                                                                                                                   <  2:1602841/148‑1 (MQ=255)
                               gcgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAg                                                                                                                 <  1:3747795/148‑1 (MQ=255)
                                cgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                                                <  1:3059899/148‑1 (MQ=255)
                                cgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                                                <  1:2905834/148‑1 (MQ=255)
                                  gAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCg                                                                                                              >  2:3307655/1‑148 (MQ=255)
                                  gAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCg                                                                                                              >  1:3568240/1‑148 (MQ=255)
                                   aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             >  2:1962429/1‑148 (MQ=255)
                                   aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             >  2:1223257/1‑148 (MQ=255)
                                   aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             <  2:2680608/148‑1 (MQ=255)
                                   aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             <  2:759113/148‑1 (MQ=255)
                                   aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGGTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             >  1:1490211/1‑148 (MQ=255)
                                       gATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                                                         >  1:2762372/1‑148 (MQ=255)
                                        atatTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCTGACTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        <  1:269136/148‑1 (MQ=255)
                                              aaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAac                                                                                                  <  2:848524/148‑1 (MQ=255)
                                              aaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAac                                                                                                  <  2:1356555/148‑1 (MQ=255)
                                                      cgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                          >  1:527132/1‑148 (MQ=255)
                                                      cgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                          >  1:1129912/1‑148 (MQ=255)
                                                          aTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                      >  1:1307528/1‑148 (MQ=255)
                                                                ttCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGCCTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACa                                                                                >  1:356711/1‑148 (MQ=255)
                                                                    ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                            >  1:1893650/1‑148 (MQ=255)
                                                                    ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                            >  2:1501101/1‑148 (MQ=255)
                                                                     gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                                           >  2:2321523/1‑148 (MQ=255)
                                                                        caaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTc                                                                        >  1:111516/1‑148 (MQ=255)
                                                                        caaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTc                                                                        >  2:454434/1‑148 (MQ=255)
                                                                        caaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTc                                                                        >  2:1649289/1‑148 (MQ=255)
                                                                          aacaGCTCGCGTGCGGCCTGTACGTACACATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCaa                                                                      <  2:3739619/148‑1 (MQ=255)
                                                                           acaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                     >  2:1117441/1‑148 (MQ=255)
                                                                                 cgcgTGCGGCATTTACGTACTCATCAGTACGCATATACACAATGTTATAGAGACTGATCAGGTAT‑CTTTTTATATCGCCAAGGAAATAACGAGAAAAGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACa                                                               <  1:1595580/148‑1 (MQ=255)
                                                                                  gcgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAt                                                              <  2:3447051/148‑1 (MQ=255)
                                                                                       gggCCTTTACTTACTAATCATTACTAATGTTAAAATTTTTATAGACACTGATCTGGTAT‑ATATTTATATCGGAACGGATATAACTACAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGcc                                                         <  2:3520168/147‑1 (MQ=255)
                                                                                       cGGCCTGTACGTACTCATCAGTACGCATCTCCACAATGTTATAGAGACTGATCAGGGCT‑CTATTGATATCGGCAAGGATATAACGAGAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAACAGGCTCAACCAGACATTCGcc                                                         <  2:2656404/148‑1 (MQ=255)
                                                                                        ggCCTTTACGTACTCATCAGTACGCATCTACACAATGTTATAGAGACTTATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGccc                                                        <  2:1810154/148‑1 (MQ=255)
                                                                                        ggCCTGTACGTACTCAACAGTACGCATCTTCACAATGTTATAGAGACTGATCCGGTCT‑CTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGccc                                                        <  2:3700511/148‑1 (MQ=255)
                                                                                         gCCTGTACGTAATATTCAGTACGCATCTTCACAATGTTATAGAGACTTATCTGGTCT‑CTTTTGATATCGGCATGTATATAACGATAAAAGTCGGTTTTGAGAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                                                       <  2:650725/148‑1 (MQ=255)
                                                                                           cTGTACGTACTCATCTGTACGAATCTTCAAAATGTTTTAGATCCTGATCAGGTCT‑ATTTTTATATCGGCAAGGATATACCGAGAAAAGTGGGTGTTGAGAAAAACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTg                                                     <  2:974611/148‑1 (MQ=255)
                                                                                           cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTg                                                     <  2:1864043/148‑1 (MQ=255)
                                                                                           cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTg                                                     <  2:1864083/148‑1 (MQ=255)
                                                                                            tGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                                                    >  1:1604376/1‑148 (MQ=255)
                                                                                             gtacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                   >  1:3519722/1‑148 (MQ=255)
                                                                                                cgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                                                <  2:1543003/148‑1 (MQ=255)
                                                                                                  tacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAt                                              >  1:1016182/1‑148 (MQ=255)
                                                                                                    cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                                            >  2:224583/1‑148 (MQ=255)
                                                                                                     tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTTTTGATATCGGCAAGGATATAACGACAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGcc                                           <  2:1085742/148‑1 (MQ=255)
                                                                                                       atcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                         >  2:1327892/1‑148 (MQ=255)
                                                                                                         caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       >  1:1356122/1‑148 (MQ=255)
                                                                                                          aGTACGAATCTACACATTGTTATAGAGACTGATCAGGTCT‑CTTTTTATATCGCCAAGTATATAACGACAAAAGTGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTa                                      <  1:2769645/148‑1 (MQ=255)
                                                                                                           gaccGCATCTTCACAATGTTATAGACACTGATCCGGTCT‑CTTTTGATATCGCCCAGTATATAACGATAAATGTCGGTGTTCACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa                                     <  1:3290567/145‑1 (MQ=255)
                                                                                                            tACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                    >  1:936889/1‑148 (MQ=255)
                                                                                                            tACGAATCTTCACATTTTTATAGAGACTGTTCCGGTAT‑CTTTTTTTTTCGGAATGTATATAACTATAATAGTGGGTTTTGAGAACCCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                    <  1:2287375/148‑1 (MQ=255)
                                                                                                                 aTCTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcat                               <  2:2450763/148‑1 (MQ=255)
                                                                                                                   cTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACTAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca                             >  2:1820937/1‑148 (MQ=255)
                                                                                                                   cTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca                             >  2:3711429/1‑148 (MQ=255)
                                                                                                                   cTTCACAATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca                             >  1:2989286/1‑148 (MQ=255)
                                                                                                                      cacaATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                          <  2:2056149/148‑1 (MQ=255)
                                                                                                                       acaATTTTATAGATACTGATCAGGTCT‑TTATTGATATCGGCATGTATATAACGATAAAAGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCa                         <  1:2850109/148‑1 (MQ=255)
                                                                                                                       acaATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCa                         >  2:1712847/1‑148 (MQ=255)
                                                                                                                        caATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAg                        >  2:742650/1‑148 (MQ=255)
                                                                                                                        caATGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAg                        >  1:2369764/1‑148 (MQ=255)
                                                                                                                         ccggTTCTAGAGACTGAAC‑CGTCGTCGATTGATATGGGAAAGGATTTAAGGAGAAAACACCGTGTTGAGAAAGACCGACCCGGCACCTACCAATCGCTCAACGATAGATTCGCCATTGGGCAAATGCCTTTTAATATCATCAAGCAgg                       <  2:2030564/145‑1 (MQ=255)
                                                                                                                         attGTTATTGTGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                       <  2:1515707/146‑1 (MQ=255)
                                                                                                                          aTGTTATAGCGACTGATCAGGTCT‑ATATTGATATCGGCATGTATATAACGACAAACGTCGGTTTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      <  1:2235622/148‑1 (MQ=255)
                                                                                                                          aTGTTATAGAGACTGATCTGGTCT‑CTTTTTATATCGGCAAGCATATAACGAGAAAAGTCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      <  1:2235863/148‑1 (MQ=255)
                                                                                                                          aTGTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      >  1:396379/1‑148 (MQ=255)
                                                                                                                            gTTATAGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGa                    <  2:399947/148‑1 (MQ=255)
                                                                                                                              tataGAGACTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                  >  1:2562887/1‑148 (MQ=255)
                                                                                                                                  gagaCGTATCTGGTCT‑CTATGGATAGGGCCAAGGAAAAAACTACAATAGTGGGTGTTGAGAAAACCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTg              <  1:2752224/148‑1 (MQ=255)
                                                                                                                                   agaCTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGc             >  2:1964730/1‑148 (MQ=255)
                                                                                                                                   agaCTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTGTAATATCATCAAGCAGGGGATCCTTGc             >  2:2918660/1‑148 (MQ=255)
                                                                                                                                     aCTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGccc           >  1:2442799/1‑148 (MQ=255)
                                                                                                                                     aCTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGccc           >  2:1571461/1‑148 (MQ=255)
                                                                                                                                      cTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc          <  2:3686003/148‑1 (MQ=255)
                                                                                                                                      cTGATCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTGGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc          >  2:3102568/1‑148 (MQ=255)
                                                                                                                                         aTCAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg       >  2:252080/1‑148 (MQ=255)
                                                                                                                                           cAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc     >  1:3786391/1‑148 (MQ=255)
                                                                                                                                           cAGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc     >  2:343149/1‑148 (MQ=255)
                                                                                                                                            aGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGGCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGccc    >  2:1231477/1‑148 (MQ=255)
                                                                                                                                            aGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGccc    >  1:2500753/1‑148 (MQ=255)
                                                                                                                                            aGGTCT‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGccc    >  2:1229187/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCG‑CTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508957‑3509246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  >  CP009273/3508953‑3509251
                                                                                                                                                     |                                                                                                                                                     
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGNACTGATAGTAAACCTCGGCGCAATTTGTTNCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTANGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       >  SRR19973069.1293703/1‑150 (MQ=60)
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       <  SRR19973069.814200/150‑1 (MQ=60)
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       <  SRR19973069.1067522/150‑1 (MQ=60)
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       >  SRR19973069.1293509/1‑150 (MQ=60)
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       >  SRR19973069.3333292/1‑150 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                     <  SRR19973069.369442/150‑1 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                     <  SRR19973069.1477981/150‑1 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                     <  SRR19973069.2800871/150‑1 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCTGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                   >  SRR19973069.2610586/1‑150 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                   >  SRR19973069.2159136/1‑150 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                   >  SRR19973069.2070410/1‑150 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                   >  SRR19973069.174035/1‑150 (MQ=60)
      AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                                 <  SRR19973069.1510633/150‑1 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCCGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                              >  SRR19973069.2101534/1‑150 (MQ=60)
          GATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATAT                                                                                                                                             <  SRR19973069.2716600/150‑1 (MQ=60)
                  CTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGG                                                                                                                                     >  SRR19973069.197446/1‑150 (MQ=60)
                         TGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAAC                                                                                                                              >  SRR19973069.2168753/1‑150 (MQ=60)
                          GAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACG                                                                                                                             >  SRR19973069.655048/1‑150 (MQ=60)
                          GAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACG                                                                                                                             <  SRR19973069.2149194/150‑1 (MQ=60)
                            ACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAG                                                                                                                           >  SRR19973069.457287/1‑150 (MQ=60)
                            ACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAG                                                                                                                           >  SRR19973069.457279/1‑150 (MQ=60)
                               CTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAA                                                                                                                        <  SRR19973069.2123341/150‑1 (MQ=60)
                                   GCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTC                                                                                                                    <  SRR19973069.3772203/150‑1 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                   <  SRR19973069.2925045/150‑1 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                   <  SRR19973069.3080138/150‑1 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                   <  SRR19973069.2691971/150‑1 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                   >  SRR19973069.3591481/1‑150 (MQ=60)
                                     GGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGGTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGG                                                                                                                  >  SRR19973069.1500561/1‑150 (MQ=60)
                                        ACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGT                                                                                                               <  SRR19973069.834092/150‑1 (MQ=60)
                                         CTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTT                                                                                                              >  SRR19973069.2780808/1‑150 (MQ=60)
                                            ATATTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCTGACTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                           <  SRR19973069.271046/150‑1 (MQ=60)
                                                  AACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAC                                                                                                     >  SRR19973069.362194/1‑150 (MQ=60)
                                                        GGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCC                                                                                               >  SRR19973069.530855/1‑150 (MQ=60)
                                                        GGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCC                                                                                               >  SRR19973069.1137792/1‑150 (MQ=60)
                                                            CAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCA                                                                                           >  SRR19973069.1316456/1‑150 (MQ=60)
                                                                  GTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGCCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACA                                                                                     >  SRR19973069.359266/1‑150 (MQ=60)
                                                                  GTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACA                                                                                     >  SRR19973069.2944976/1‑150 (MQ=60)
                                                                      CGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAG                                                                                 >  SRR19973069.1559377/1‑150 (MQ=60)
                                                                      CGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAG                                                                                 >  SRR19973069.1906770/1‑150 (MQ=60)
                                                                         GAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCT                                                                              >  SRR19973069.3823835/1‑150 (MQ=60)
                                                                          AACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTC                                                                             >  SRR19973069.112610/1‑150 (MQ=60)
                                                                                     CGCGTGCGGCATTTACGTACTCATCAGTACGCATATACACAATGTTATAGAGACTGATCAGGTATCTTTTTATATCGCCAAGGAAATAACGAGAAAAGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATT                                                                  <  SRR19973069.1606351/150‑1 (MQ=60)
                                                                                      gcgtggggcgtttcgttactaatctgaaggaatatacacatttTTATAGAGACTTATCCGGTCTCTATTTATAGCGCCAAGTATATACCGACAAATGTGGGTGTTGAGAACCACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTC                                                                 <  SRR19973069.3105422/107‑1 (MQ=43)
                                                                                              CCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGG                                                         >  SRR19973069.1615176/1‑150 (MQ=60)
                                                                                               CTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGG                                                        >  SRR19973069.3542457/1‑150 (MQ=60)
                                                                                                    CGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAT                                                   >  SRR19973069.1023192/1‑150 (MQ=60)
                                                                                                           ATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTT                                            >  SRR19973069.1286934/1‑150 (MQ=60)
                                                                                                           ATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTT                                            >  SRR19973069.2298581/1‑150 (MQ=60)
                                                                                                           ATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTT                                            >  SRR19973069.1365540/1‑150 (MQ=60)
                                                                                                              AGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAT                                         >  SRR19973069.943232/1‑150 (MQ=60)
                                                                                                              AGTACGAATCTACACATTGTTATAGAGACTGATCAGGTCTCTTTTTATATCGCCAAGTATATAACGACAAAAGTGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAT                                         <  SRR19973069.2788102/150‑1 (MQ=60)
                                                                                                               gacCGCATCTTCACAATGTTATAGACACTGATCCGGTCTCTTTTGATATCGCCCAGTATATAACGATAAATGTCGGTGTTCACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATA                                        <  SRR19973069.3312111/147‑1 (MQ=60)
                                                                                                                  CGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCA                                     >  SRR19973069.1718752/1‑150 (MQ=60)
                                                                                                                     ATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCA                                  >  SRR19973069.3009135/1‑150 (MQ=60)
                                                                                                                          CACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAG                             >  SRR19973069.2385847/1‑150 (MQ=60)
                                                                                                                          CACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAG                             >  SRR19973069.2386130/1‑150 (MQ=60)
                                                                                                                           ACAATTTTATAGATACTGATCAGGTCTTTATTGATATCGGCATGTATATAACGATAAAAGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGG                            <  SRR19973069.2868879/150‑1 (MQ=60)
                                                                                                                            CAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGG                           >  SRR19973069.399284/1‑150 (MQ=60)
                                                                                                                              ATGTTATAGCGACTGATCAGGTCTATATTGATATCGGCATGTATATAACGACAAACGTCGGTTTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGA                         <  SRR19973069.2250704/150‑1 (MQ=60)
                                                                                                                              ATGTTATAGAGACTGATCTGGTCTCTTTTTATATCGGCAAGCATATAACGAGAAAAGTCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGA                         <  SRR19973069.2250945/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                       >  SRR19973069.2579810/1‑150 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                       >  SRR19973069.2579686/1‑150 (MQ=60)
                                                                                                                                       AGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCC                >  SRR19973069.2459244/1‑150 (MQ=60)
                                                                                                                                           TGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTG            >  SRR19973069.3239818/1‑150 (MQ=60)
                                                                                                                                             ATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC          >  SRR19973069.3810955/1‑150 (MQ=60)
                                                                                                                                              TCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCC         >  SRR19973069.2517454/1‑150 (MQ=60)
                                                                                                                                               CAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCA        >  SRR19973069.2755816/1‑150 (MQ=60)
                                                                                                                                                AGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC       >  SRR19973069.3571794/1‑150 (MQ=60)
                                                                                                                                                AGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCGACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC       >  SRR19973069.1442543/1‑150 (MQ=60)
                                                                                                                                                  GTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT     >  SRR19973069.381440/1‑150 (MQ=60)
                                                                                                                                                   TCTCTTTTGATATCGGCAAGTATATAACGAGAAATGTCGGTGTTGAGAAAAACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTC    <  SRR19973069.2514156/150‑1 (MQ=60)
                                                                                                                                                   TCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTC    >  SRR19973069.2587659/1‑150 (MQ=60)
                                                                                                                                                    CTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGTGATACTTGCCCCCTGCCCACTTCA   >  SRR19973069.2965844/1‑150 (MQ=60)
                                                                                                                                                    CTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGTGATACTTGCCCCCTGCCCACTTCA   >  SRR19973069.1045867/1‑150 (MQ=60)
                                                                                                                                                    CTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGTGATACTTGCCCCCTGCCCACTTCA   >  SRR19973069.1045611/1‑150 (MQ=60)
                                                                                                                                                     TCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  >  SRR19973069.572765/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  >  CP009273/3508953‑3509251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑