Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,509,102 G→T 100% S57R (AGC→AGA)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,509,1020GT98.6% 886.6 / ‑0.4 282S57R (AGC→AGA) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (136/142);  total (138/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 286 total aligned reads displayed.

ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248
                                                                                                                                                   |                                                                                                                                                  
 cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                                 >  2:797926/1‑150 (MQ=255)
 cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                                 >  1:201738/1‑150 (MQ=255)
  ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                                >  2:3750609/1‑150 (MQ=255)
  ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                                >  2:3823579/1‑150 (MQ=255)
      cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGat                                                                                                                                            <  1:3104091/150‑1 (MQ=255)
        gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGatat                                                                                                                                          <  2:3247129/150‑1 (MQ=255)
          tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCg                                                                                                                                        <  2:4096435/150‑1 (MQ=255)
           ccTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCgg                                                                                                                                       <  2:4984467/150‑1 (MQ=255)
              ggCTTTTGTTGAACTCTTCGCTGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCaa                                                                                                                                    <  1:1044323/150‑1 (MQ=255)
               gCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAg                                                                                                                                   >  2:4134908/1‑150 (MQ=255)
                cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAgg                                                                                                                                  <  2:2576184/150‑1 (MQ=255)
                  tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGat                                                                                                                                <  1:889753/150‑1 (MQ=255)
                   ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGata                                                                                                                               <  1:386535/150‑1 (MQ=255)
                    tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGatat                                                                                                                              <  2:2246279/150‑1 (MQ=255)
                    tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGatat                                                                                                                              <  2:1090234/150‑1 (MQ=255)
                    tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGatat                                                                                                                              <  2:4899432/150‑1 (MQ=255)
                     gttgAACTCGTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGAtata                                                                                                                             <  1:2451213/150‑1 (MQ=255)
                      ttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATaa                                                                                                                            <  2:1707101/150‑1 (MQ=255)
                      ttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATaa                                                                                                                            <  2:4385660/150‑1 (MQ=255)
                       tgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAc                                                                                                                           >  2:4590188/1‑150 (MQ=255)
                       tgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAc                                                                                                                           <  2:4752415/150‑1 (MQ=255)
                         aaCTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACga                                                                                                                         >  2:514328/1‑150 (MQ=255)
                          aCTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGCTATAACgag                                                                                                                        >  1:1513266/1‑150 (MQ=255)
                          aCTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACgag                                                                                                                        >  2:4409701/1‑150 (MQ=255)
                             ctTCGCGGAACTGATAGTAAACCTCGGCTCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGTCCTGTAAGTACTCATCAGTACGAATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAATGATATAACGAGaaa                                                                                                                     <  2:3058501/150‑1 (MQ=255)
                                  cgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCg                                                                                                                >  1:3044333/1‑150 (MQ=255)
                                      aCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                            >  1:417947/1‑150 (MQ=255)
                                       cTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                                                           <  2:2863622/150‑1 (MQ=255)
                                          aTAGTATACCTCGTCGCATTTTGTTTCGTGAACAAACAGCTAGCGTGCGGGCTGTTCTTACTCATCTGTACGAATCTTCACAATGTTATAGAGACTGTTCAGGTCTCTATTGATATCGGCAAGGTTATAACGAGATAAGTCGGTGTTgag                                                                                                        <  1:384289/150‑1 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        >  2:2474449/1‑150 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        <  2:261544/150‑1 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        <  1:5151014/150‑1 (MQ=255)
                                            aGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                      >  2:2087568/1‑150 (MQ=255)
                                             gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGCCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGgaa                                                                                                     >  1:4578445/1‑150 (MQ=255)
                                             gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                     <  1:54108/150‑1 (MQ=255)
                                                  ccTCGGCTCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTTCTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCg                                                                                                <  1:2603939/150‑1 (MQ=255)
                                                    tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAc                                                                                              <  1:2082714/150‑1 (MQ=255)
                                                      ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                            <  2:1379518/150‑1 (MQ=255)
                                                       gcgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCg                                                                                           <  2:1792540/150‑1 (MQ=255)
                                                         gcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGc                                                                                         <  2:3776125/150‑1 (MQ=255)
                                                           aaTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                       >  1:132303/1‑150 (MQ=255)
                                                           aaTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                       >  2:491841/1‑150 (MQ=255)
                                                                     gggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                                             >  1:1405639/1‑150 (MQ=255)
                                                                           aaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                       >  2:3168767/1‑150 (MQ=255)
                                                                            aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                      >  1:4045630/1‑150 (MQ=255)
                                                                                  tCGCGTGCGGCCTGTACTTACTAATCATTACGCATATTCACAATGTTATAGAGACTGATCTGGTCTCTATTTATATCGGAAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAt                                                                <  1:4838913/150‑1 (MQ=255)
                                                                                         cGGCATGTACGTACTAATAAGTACGAATATTCAAAATTTTATATAGAATGATATGTTATCTATTGATATCGTAAAGTATATAACTATAAAAGTGGGTGTTTAGAAACACCGACCCGGCACCTACAAAATGCTCAACCAGACATTCGCCCt                                                         <  2:3599871/150‑1 (MQ=255)
                                                                                          ggCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCtt                                                        >  1:2394540/1‑150 (MQ=255)
                                                                                            cgtttacttactAATCTTTACTAATATTAACATTTTTATTTAGACTTATCTGGTATATTTTTATATCGGAACGTATATAACTATAAATTTGGTTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                                                      <  1:4219019/146‑1 (MQ=255)
                                                                                            ccTGTACGTACTCAACAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                                                      <  2:588645/150‑1 (MQ=255)
                                                                                             ctttacgtaaTCATCAGTACGAATCTTCACAATGTTATAGAGACTGATCAGGTATCTATTGATATCGCCAAGGATATAACGATAAATGTGGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                     <  1:5236793/147‑1 (MQ=255)
                                                                                             cTGTACTTACTCATCTTTACGAATCTTCACAATTTTATAGAGACTGATCAGGTCTCTTTTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                     <  2:5073718/150‑1 (MQ=255)
                                                                                              tGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAACAGGCTCAACCAGACATTCGCCCTTGGGc                                                    >  2:4299267/1‑150 (MQ=255)
                                                                                               gtacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCa                                                   >  1:59105/1‑150 (MQ=255)
                                                                                                  cgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAt                                                >  2:4672722/1‑150 (MQ=255)
                                                                                                   ttacTCATATTTACGAAACTTCAAATTGTTATCGGTACTGATCTGGTCTCTTTTTATAGCGCCAAGTATATAACGTGAAATGTCGGTGTTGACAAACACCCACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                                               <  2:2323647/149‑1 (MQ=255)
                                                                                                       tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                           >  1:1037610/1‑150 (MQ=255)
                                                                                                        catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                          >  2:2895751/1‑150 (MQ=255)
                                                                                                         atcaGTCCGCATCTACACAATGTGATAGAGACTGATCCGGTCTCTATTGATATCGGCAAGGATATACCGCGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                         <  2:1159769/150‑1 (MQ=255)
                                                                                                         atcaGTACGCATCTTCACAATGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGcc                                             <  2:1615755/146‑1 (MQ=255)
                                                                                                         atcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGcc                                             >  1:1615755/1‑146 (MQ=255)
                                                                                                         atcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                         >  2:1488144/1‑150 (MQ=255)
                                                                                                            aGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTCTTGATATCGGCAAGGATATCACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                      >  1:2347826/1‑150 (MQ=255)
                                                                                                               aCGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatc                                   >  2:4815001/1‑150 (MQ=255)
                                                                                                                cGCATCTCCACAATTTTATCGAGACTGAACTGGTCTCTATTGATATCGGCAAGCATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca                                  <  1:2948039/150‑1 (MQ=255)
                                                                                                                cGCAACTTCACATTGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca                                  <  1:2179228/150‑1 (MQ=255)
                                                                                                                 gCATCTTCACCTGGTTATAGTGACTGATCCGGTCTATTTTGATATGGGCAAGGTTTTACCGAGAAATGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcat                                 <  1:3229042/150‑1 (MQ=255)
                                                                                                                   aTCTTCACAATGTTATTGAGACTGATCTGGTCTCTTTTGATATCGGCAAGTATATACCGAGAAAAGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca                               <  2:1930377/150‑1 (MQ=255)
                                                                                                                     cTTCACAATGTTATAGAGACTTATCAGGTCTCTATTGATATATGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGTCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                             <  2:701953/150‑1 (MQ=255)
                                                                                                                     cTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAATTCGGTGTTGAGAAACACCGACACGGCACCTACAAAAGGCTCAACCATACATTCGCCCTTGGGCAAATGCAGTTTATTATCATCAAg                             >  1:749614/1‑150 (MQ=255)
                                                                                                                     cTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                             >  1:2959828/1‑150 (MQ=255)
                                                                                                                      ttaaaattGTTATAGAGACTGAACTGGTATATTTTTATTTCGGAAAGTATATAACTATAAATGTCGGTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                            <  1:3750609/143‑1 (MQ=255)
                                                                                                                      ttCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                            >  2:111233/1‑150 (MQ=255)
                                                                                                                      ttCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                            >  2:248010/1‑150 (MQ=255)
                                                                                                                       tCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCACATGCCGTTTAATATCATCAAGCa                           >  2:2779227/1‑150 (MQ=255)
                                                                                                                       tCACAATGTTATAGAGACTGAACAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCa                           <  1:2689517/150‑1 (MQ=255)
                                                                                                                        cacaATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAg                          >  2:2368470/1‑150 (MQ=255)
                                                                                                                         acaATGTTATAGAGACTGAACCGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                         <  1:4887239/150‑1 (MQ=255)
                                                                                                                          caATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                        >  1:1951389/1‑150 (MQ=255)
                                                                                                                           aaTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCACATGCCGTTTAATATCAGCAAGCAgggg                       >  1:2321065/1‑150 (MQ=255)
                                                                                                                             ttttATCGAGACTTATCTGTTCTATTTTTATATCGGCATTTATATAACTATAAATGTGGGTTTTTACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAt                     <  1:510876/148‑1 (MQ=255)
                                                                                                                              tttATAGAGACTGATCAGTTCTATTTTTATATCGGAATGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                    <  1:5193028/149‑1 (MQ=255)
                                                                                                                              gTTTTAGAGACTGATCTGGTCTCTATTGTTATCGGCATGGATATAACGAGAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                    <  1:3511925/150‑1 (MQ=255)
                                                                                                                              gTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGATAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                    <  1:3440704/150‑1 (MQ=255)
                                                                                                                              gTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                    >  2:2938664/1‑150 (MQ=255)
                                                                                                                               ttATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATAc                   <  1:362563/150‑1 (MQ=255)
                                                                                                                                 atatacaCTGATCAGGTATCTATTTATATCGCCATGTATATAACGATAAAAGTCGTTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACtt                 <  1:663235/144‑1 (MQ=255)
                                                                                                                                 atatAGACTTATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACtt                 <  1:2126637/150‑1 (MQ=255)
                                                                                                                                 ataGATACTGATCAGGTCTATTTTTATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGCCCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACtt                 <  1:1145365/150‑1 (MQ=255)
                                                                                                                                  taGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTg                >  1:467993/1‑150 (MQ=255)
                                                                                                                                   agagACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGc               >  1:1803042/1‑150 (MQ=255)
                                                                                                                                     agaCTGATCAGGTCTCTATTGATATCGGCAAGGATCTAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCCAATGCCGTGTAATATCATCAAGCAGGGGCTACTTGccc             >  2:867310/1‑150 (MQ=255)
                                                                                                                                     agaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGccc             >  1:4466742/1‑150 (MQ=255)
                                                                                                                                     agaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCACAACCACACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTAGccc             >  1:2497273/1‑150 (MQ=255)
                                                                                                                                        cTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCt          <  2:1248894/150‑1 (MQ=255)
                                                                                                                                          gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc        >  2:2012226/1‑150 (MQ=255)
                                                                                                                                          gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc        >  2:3435574/1‑150 (MQ=255)
                                                                                                                                          gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTGTAATATCATCAAGCAGGGGATACTTGCCCCCTGc        >  1:1920392/1‑150 (MQ=255)
                                                                                                                                             cAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCa     >  2:4923704/1‑150 (MQ=255)
                                                                                                                                              aGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAc    >  2:3634444/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCA  >  CP009273/3508953‑3509250
                                                                                                                                                     |                                                                                                                                                    
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                      <  SRR19973062.3742275/150‑1 (MQ=60)
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                      >  SRR19973062.5217382/1‑150 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                    <  SRR19973062.4133217/150‑1 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                    <  SRR19973062.1456040/150‑1 (MQ=60)
   CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTA                                                                                                                                                   >  SRR19973062.202822/1‑150 (MQ=60)
   CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTA                                                                                                                                                   <  SRR19973062.1052488/150‑1 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGTGACTGATCAGGTCTCTAT                                                                                                                                                  >  SRR19973062.2335931/1‑150 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                  >  SRR19973062.1099296/1‑150 (MQ=60)
    GGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAT                                                                                                                                                  >  SRR19973062.3008253/1‑150 (MQ=60)
     GAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATT                                                                                                                                                 <  SRR19973062.1253375/150‑1 (MQ=60)
     GAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATT                                                                                                                                                 <  SRR19973062.657959/150‑1 (MQ=60)
      AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                                <  SRR19973062.356623/150‑1 (MQ=60)
        CGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGAT                                                                                                                                              <  SRR19973062.3120082/150‑1 (MQ=60)
        CGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGAT                                                                                                                                              <  SRR19973062.3108187/150‑1 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                             <  SRR19973062.153113/150‑1 (MQ=60)
          gttgCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTTCGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATAT                                                                                                                                            <  SRR19973062.74432/146‑1 (MQ=60)
           ATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATC                                                                                                                                           <  SRR19973062.1473634/150‑1 (MQ=60)
           ATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATC                                                                                                                                           <  SRR19973062.1866953/150‑1 (MQ=60)
            TCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCG                                                                                                                                          <  SRR19973062.4817737/150‑1 (MQ=60)
                GGCTTTTGTTGAACTCTTCGCTGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAA                                                                                                                                      <  SRR19973062.1049227/150‑1 (MQ=60)
                  CTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGG                                                                                                                                    >  SRR19973062.5187770/1‑150 (MQ=60)
                   TTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGA                                                                                                                                   <  SRR19973062.1354654/150‑1 (MQ=60)
                   TTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGA                                                                                                                                   <  SRR19973062.2461665/150‑1 (MQ=60)
                    TTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGAT                                                                                                                                  <  SRR19973062.3578801/150‑1 (MQ=60)
                    TTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGAT                                                                                                                                  <  SRR19973062.893560/150‑1 (MQ=60)
                     TTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATA                                                                                                                                 <  SRR19973062.388187/150‑1 (MQ=60)
                     TTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATA                                                                                                                                 <  SRR19973062.388254/150‑1 (MQ=60)
                     TTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATA                                                                                                                                 <  SRR19973062.2210542/150‑1 (MQ=60)
                      TGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATAT                                                                                                                                <  SRR19973062.3807768/150‑1 (MQ=60)
                      TGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATAT                                                                                                                                >  SRR19973062.262531/1‑150 (MQ=60)
                       GTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCTTCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATA                                                                                                                               <  SRR19973062.2464188/150‑1 (MQ=60)
                       GTTGAACTCGTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATA                                                                                                                               <  SRR19973062.2464369/150‑1 (MQ=60)
                        TTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAA                                                                                                                              <  SRR19973062.5131558/150‑1 (MQ=60)
                         TGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAC                                                                                                                             <  SRR19973062.5160000/150‑1 (MQ=60)
                           AACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACACATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGA                                                                                                                           <  SRR19973062.3145733/150‑1 (MQ=60)
                            ACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGCTATAACGAG                                                                                                                          >  SRR19973062.1520721/1‑150 (MQ=60)
                               CTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAA                                                                                                                       >  SRR19973062.583496/1‑150 (MQ=60)
                                TTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAA                                                                                                                      <  SRR19973062.2024409/150‑1 (MQ=60)
                                   GCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTC                                                                                                                   <  SRR19973062.4663056/150‑1 (MQ=60)
                                   GCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTC                                                                                                                   <  SRR19973062.930152/150‑1 (MQ=60)
                                   GCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTC                                                                                                                   <  SRR19973062.1262788/150‑1 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                  >  SRR19973062.464676/1‑150 (MQ=60)
                                    CGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCG                                                                                                                  >  SRR19973062.3060162/1‑150 (MQ=60)
                                     GGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGG                                                                                                                 >  SRR19973062.382633/1‑150 (MQ=60)
                                        ACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGT                                                                                                              >  SRR19973062.419749/1‑150 (MQ=60)
                                        ACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGT                                                                                                              >  SRR19973062.2098079/1‑150 (MQ=60)
                                          TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                            <  SRR19973062.2255909/150‑1 (MQ=60)
                                          TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                            >  SRR19973062.2159118/1‑150 (MQ=60)
                                           GATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGA                                                                                                           >  SRR19973062.3872228/1‑150 (MQ=60)
                                            ATAGTATACCTCGTCGCATTTTGTTTCGTGAACAAACAGCTAGCGTGCGGGCTGTTCTTACTCATCTGTACGAATCTTCACAATGTTATAGAGACTGTTCAGGTCTCTATTGATATCGGCAAGGTTATAACGAGATAAGTCGGTGTTGAG                                                                                                          <  SRR19973062.386004/150‑1 (MQ=60)
                                            ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                          <  SRR19973062.5178238/150‑1 (MQ=60)
                                              AGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGaaagcagaat                                                                                                        >  SRR19973062.2239713/1‑140 (MQ=60)
                                               GTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGCCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGGAA                                                                                                       >  SRR19973062.4602381/1‑150 (MQ=60)
                                               GTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAA                                                                                                       <  SRR19973062.54492/150‑1 (MQ=60)
                                                TAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAAC                                                                                                      <  SRR19973062.4958006/150‑1 (MQ=60)
                                                  AACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAC                                                                                                    >  SRR19973062.5250833/1‑150 (MQ=60)
                                                   ACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACC                                                                                                   >  SRR19973062.1622782/1‑150 (MQ=60)
                                                    CCTCGGCTCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTTCTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCG                                                                                                  <  SRR19973062.2617712/150‑1 (MQ=60)
                                                      TCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAC                                                                                                <  SRR19973062.2093482/150‑1 (MQ=60)
                                                      TCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAC                                                                                                <  SRR19973062.2093370/150‑1 (MQ=60)
                                                           GCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGC                                                                                           <  SRR19973062.5348574/150‑1 (MQ=60)
                                                            CAATTTGTTTCGGGAACAAACAGCTCTCGTGCGGCCTGTACGTACTCATCAGTACGAATCTTCACAATGTTATAGATACTGATCAGGTCTCTATTTATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCA                                                                                          >  SRR19973062.3465167/1‑150 (MQ=60)
                                                            CAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCA                                                                                          >  SRR19973062.1940276/1‑150 (MQ=60)
                                                             AATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAC                                                                                         >  SRR19973062.133092/1‑150 (MQ=60)
                                                             AATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAC                                                                                         >  SRR19973062.133128/1‑150 (MQ=60)
                                                                  GTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACA                                                                                    >  SRR19973062.3741769/1‑150 (MQ=60)
                                                                    TTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAA                                                                                  >  SRR19973062.2919764/1‑150 (MQ=60)
                                                                      CGGGAAAAAACAGCTCGCGTGCGGCCTGTACGTACTAATCAGTACGAATCTACACAATGTTATAGTGACTTATCTGGTCTCTATTGATATCGGCAAGGATATAACGATAAATGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAG                                                                                <  SRR19973062.828883/150‑1 (MQ=60)
                                                                       GGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGG                                                                               >  SRR19973062.1412356/1‑150 (MQ=60)
                                                                        GGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGC                                                                              >  SRR19973062.2004696/1‑150 (MQ=60)
                                                                          AACAAACCGCTCGCGTGCGGCCTGTACGTAATCATCTGTACGAATCTTCACAATGTTATAGTGACTGATCTGGTCTCTTTTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTC                                                                            <  SRR19973062.3954943/150‑1 (MQ=60)
                                                                          AACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGTCTC                                                                            >  SRR19973062.296891/1‑150 (MQ=60)
                                                                             AAACAGCTCGCGGGCGCCCTGTACGTACCCAACAGTACCCATCTTCACATGTTTATCTAGACTGATCAGGTCTCTTTTGATATCCGCACGGATATACCTACAAAAGCGGGTGTTGAGAACCCCCGACCCGGCACCTACAAAAGGCTCACC                                                                         <  SRR19973062.1074198/150‑1 (MQ=28)
                                                                              AACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACC                                                                        >  SRR19973062.4066291/1‑150 (MQ=60)
                                                                                CAGCTCGCGTGCGGCCTGTACGTACTCATCATTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCATGGATATAACGAGAAATGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAG                                                                      <  SRR19973062.1768630/150‑1 (MQ=60)
                                                                                 CGCTCGCGGGCGGCCTGTACGTACTCAACAGTACGCATATACACTTTGTTATATAGACTGCTCAGGTCTCTTTTGATATCGGCATGTATATAACGATAAATGTCGGTTTTGACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGA                                                                     <  SRR19973062.3733955/150‑1 (MQ=60)
                                                                                    TCGCGTGCGGCCTGTACTTACTAATCATTACGCATATTCACAATGTTATAGAGACTGATCTGGTCTCTATTTATATCGGAAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAT                                                                  <  SRR19973062.4863919/150‑1 (MQ=60)
                                                                                        GTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGC                                                              >  SRR19973062.4895898/1‑150 (MQ=60)
                                                                                            GGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTT                                                          >  SRR19973062.2407386/1‑150 (MQ=60)
                                                                                            GGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCGT                                                          >  SRR19973062.3089460/1‑150 (MQ=60)
                                                                                               CTTTACGTAATCATCAGTACGAATCTTCACAATGTTATAGAGACTGATCAGGTATCTATTGATATCGCCAAGGATATAACGATAAATGTGGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGG                                                       <  SRR19973062.5264381/150‑1 (MQ=60)
                                                                                               CTGTACGTACTCATATGTACGCATCTTCACAATTTTATAGAGACTGATCTGGTCTATTTTTATATCGGAAAGTATATAACGATAAATGTGGGTTTTGATAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGG                                                       <  SRR19973062.5264407/150‑1 (MQ=60)
                                                                                                 GTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCA                                                     >  SRR19973062.59428/1‑150 (MQ=60)
                                                                                                 GTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCA                                                     >  SRR19973062.59520/1‑150 (MQ=60)
                                                                                                    CGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAT                                                  >  SRR19973062.455429/1‑150 (MQ=60)
                                                                                                     gatctaATCAGTACGCATCTTCACATTGTTATAGAGACTGATCAGTTATATTTTTATATCGCCATGTATATAACGAGAAACGTGGGTGTTGACAAACCCCGACCCGGCACCTACAAAAGGCTCAACCGGACATTCGCCCTTGGGCAAATG                                                 <  SRR19973062.5142448/144‑1 (MQ=60)
                                                                                                     GTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATG                                                 >  SRR19973062.65414/1‑150 (MQ=60)
                                                                                                      TACTCATCTGTACGAATCTACCCAATGTTATCGAGACTGATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAATGGCGGTGTTTACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGC                                                <  SRR19973062.4203995/150‑1 (MQ=60)
                                                                                                         TCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGT                                             >  SRR19973062.1042485/1‑150 (MQ=60)
                                                                                                         TCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGT                                             >  SRR19973062.1042466/1‑150 (MQ=60)
                                                                                                           ATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCagat                                           >  SRR19973062.1623751/1‑146 (MQ=60)
                                                                                                              AGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTCTTGATATCGGCAAGGATATCACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAT                                        >  SRR19973062.2360323/1‑150 (MQ=60)
                                                                                                              AGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACACAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAT                                        >  SRR19973062.2360370/1‑150 (MQ=60)
                                                                                                                TACGCATCTTCACAATTTTATAGATAATTATCTGGTCTCTATTGATATCGGCTAGTATATAACTATAAAAGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAT                                      <  SRR19973062.2533021/150‑1 (MQ=60)
                                                                                                                  CGCATCTCCACAATTTTATCGAGACTGAACTGGTCTCTATTGATATCGGCAAGCATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCA                                    <  SRR19973062.2963536/150‑1 (MQ=60)
                                                                                                                  CGCAACTTCACATTGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCA                                    <  SRR19973062.2190207/150‑1 (MQ=60)
                                                                                                                   GCATCTTCACCTGGTTATAGTGACTGATCCGGTCTATTTTGATATGGGCAAGGTTTTACCGAGAAATGTGGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCAT                                   <  SRR19973062.3245801/150‑1 (MQ=60)
                                                                                                                    CATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAATGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAATGCTCAACCAGACATTCGCCCTTGGGCAAATTCCGTTAAATATCATA                                  >  SRR19973062.3952672/1‑150 (MQ=60)
                                                                                                                       ctacaaatttTTATAGAGACTGATCAGTACTATATTGATATCGCCAAGTATATAACTATAAAAGTGGGTTTTGACAACCCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG                               <  SRR19973062.1024557/140‑1 (MQ=60)
                                                                                                                       CTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAATTCGGTGTTGAGAAACACCGACACGGCACCTACAAAAGGCTCAACCATACATTCGCCCTTGGGCAAATGCAGTTTATTATCATCAAG                               >  SRR19973062.753011/1‑150 (MQ=60)
                                                                                                                       CTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG                               >  SRR19973062.4421913/1‑150 (MQ=60)
                                                                                                                       CTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG                               >  SRR19973062.1698956/1‑150 (MQ=60)
                                                                                                                       CTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG                               >  SRR19973062.2975359/1‑150 (MQ=60)
                                                                                                                       CTACACAATGTTATCGAGACTGATCAGGTCTCTATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG                               <  SRR19973062.2949579/150‑1 (MQ=60)
                                                                                                                        TTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTTATATCATCAAGC                              >  SRR19973062.3007196/1‑150 (MQ=60)
                                                                                                                        TTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGC                              >  SRR19973062.3202201/1‑150 (MQ=60)
                                                                                                                         TCACAATGTTATAGAGACTGAACAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCA                             <  SRR19973062.2703590/150‑1 (MQ=60)
                                                                                                                           ACAATGTTATAGAGACTGAACCGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGG                           <  SRR19973062.4912397/150‑1 (MQ=60)
                                                                                                                            CAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGG                          >  SRR19973062.1961411/1‑150 (MQ=60)
                                                                                                                            CAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGG                          >  SRR19973062.1961412/1‑150 (MQ=60)
                                                                                                                             AATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCACATGCCGTTTAATATCAGCAAGCAGGGG                         >  SRR19973062.2333320/1‑150 (MQ=60)
                                                                                                                             AATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGG                         >  SRR19973062.817057/1‑150 (MQ=60)
                                                                                                                              ATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGA                        >  SRR19973062.2612850/1‑150 (MQ=60)
                                                                                                                               TGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAT                       <  SRR19973062.2688085/150‑1 (MQ=60)
                                                                                                                                GTTTTAGAGACTGATCTGGTCTCTATTGTTATCGGCATGGATATAACGAGAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                      <  SRR19973062.3529725/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                      <  SRR19973062.3529570/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGATAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                      <  SRR19973062.3458282/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                      >  SRR19973062.4123081/1‑150 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCAGGCACCTACAAAAGGCTCAACCAGACATTCTCCATTGGGAAAATGCCGTTTAATATCATCAAGCAGTGGATA                      >  SRR19973062.3286630/1‑150 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGCGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                      <  SRR19973062.5220351/150‑1 (MQ=60)
                                                                                                                                 TTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATAC                     <  SRR19973062.364194/150‑1 (MQ=60)
                                                                                                                                   ATATAGACTTATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                   <  SRR19973062.2137450/150‑1 (MQ=60)
                                                                                                                                   ATATACACTGATCAGGTATCTATTTATATCGCCATGTATATAACGATAAAAGTCGTTTTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                   <  SRR19973062.666318/150‑1 (MQ=60)
                                                                                                                                   ATAGATACTGATCAGGTCTATTTTTATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGCCCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                   <  SRR19973062.1150732/150‑1 (MQ=60)
                                                                                                                                   ATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                   <  SRR19973062.2137864/150‑1 (MQ=60)
                                                                                                                                   ATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTT                   >  SRR19973062.4909340/1‑150 (MQ=60)
                                                                                                                                    TAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTG                  >  SRR19973062.343950/1‑150 (MQ=60)
                                                                                                                                    TAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTG                  >  SRR19973062.470081/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                 >  SRR19973062.2036521/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                 >  SRR19973062.1811767/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                 >  SRR19973062.71311/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCAACAAGCAGGGGATACTTGC                 >  SRR19973062.2036138/1‑150 (MQ=60)
                                                                                                                                       AGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCC               >  SRR19973062.4489571/1‑150 (MQ=60)
                                                                                                                                       AGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCC               >  SRR19973062.2397970/1‑150 (MQ=60)
                                                                                                                                       AGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCACAACCACACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTAGCCC               >  SRR19973062.2510623/1‑150 (MQ=60)
                                                                                                                                           TGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTG           >  SRR19973062.4822511/1‑150 (MQ=60)
                                                                                                                                            GATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTGTAATATCATCAAGCAGGGGATACTTGCCCCCTGC          >  SRR19973062.1930228/1‑150 (MQ=60)
                                                                                                                                                    CTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCA  >  SRR19973062.2380388/1‑150 (MQ=60)
                                                                                                                                                    CTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCA  >  SRR19973062.3479665/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCA  >  CP009273/3508953‑3509250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑